Otof gene cDNA ORF clone, Mesocricetus auratus(golden hamster)

mRNA	Protein	Name
XM_013116034.1	XP_012971488.1	otoferlin isoform X3
XM_013116036.1	XP_012971490.1	otoferlin isoform X5
XM_013116033.1	XP_012971487.1	otoferlin isoform X2
XM_021229221.1	XP_021084880.1	otoferlin isoform X1
XM_005073511.2	XP_005073568.1	otoferlin isoform X6
XM_005073511.3	XP_005073568.1	otoferlin isoform X4
XM_021229223.1	XP_021084882.1	otoferlin isoform X5
XM_013116032.1	XP_012971486.1	otoferlin isoform X1
XM_013116035.1	XP_012971489.1	otoferlin isoform X2
XM_013116036.2	XP_012971490.1	otoferlin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe127221	XM_013116033.1 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe127221	XM_021229221.1 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe127222	XM_013116034.1 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe127223	XM_013116035.1 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe127224	XM_013116036.2 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe127225	XM_005073511.3 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe249715	XM_021229223.1 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe127220	XM_013116032.1 Latest version!	Mesocricetus auratus otoferlin (Otof), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OMe127225	XM_005073511.2	Mesocricetus auratus otoferlin (Otof), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMe127224	XM_013116036.1	Mesocricetus auratus otoferlin (Otof), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

Otof ( XM_013116033.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012971487.1 Mesocricetus auratus otoferlin (Otof), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMe127221
Clone ID Related Accession (Same CDS sequence)	XM_013116033.1 , XM_021229221.1
Accession Version	XM_013116033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	otoferlin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA TCGGCCCGAC 
ACGAGCTTCA TCTGGTTCTT GAACCCTCTC AAGTCTGCCC GCTACTTCCT GTGGCACACC 
TACCGCTGGC TGCTCCTCAA ACTCCTTCTG CTGCTCCTCC TGTTGCTGCT GCTCGCCCTG 
TTTCTCTACT CTCTGCCTGG CTACCTAGCC AAGAAGATCC TGGGGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971487.1
CDS	1..5991
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTVRLLRGCHVLCNGGPSSRPTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	XM_013116033.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116033.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA TCGGCCCGAC 
ACGAGCTTCA TCTGGTTCTT GAACCCTCTC AAGTCTGCCC GCTACTTCCT GTGGCACACC 
TACCGCTGGC TGCTCCTCAA ACTCCTTCTG CTGCTCCTCC TGTTGCTGCT GCTCGCCCTG 
TTTCTCTACT CTCTGCCTGG CTACCTAGCC AAGAAGATCC TGGGGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_021229221.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021084880.1 Mesocricetus auratus otoferlin (Otof), transcript variant X1, mRNA.

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CloneID	OMe127221
Clone ID Related Accession (Same CDS sequence)	XM_013116033.1 , XM_021229221.1
Accession Version	XM_021229221.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA TCGGCCCGAC 
ACGAGCTTCA TCTGGTTCTT GAACCCTCTC AAGTCTGCCC GCTACTTCCT GTGGCACACC 
TACCGCTGGC TGCTCCTCAA ACTCCTTCTG CTGCTCCTCC TGTTGCTGCT GCTCGCCCTG 
TTTCTCTACT CTCTGCCTGG CTACCTAGCC AAGAAGATCC TGGGGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021084880.1
CDS	1..5991
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTVRLLRGCHVLCNGGPSSRPTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	XM_021229221.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021229221.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA TCGGCCCGAC 
ACGAGCTTCA TCTGGTTCTT GAACCCTCTC AAGTCTGCCC GCTACTTCCT GTGGCACACC 
TACCGCTGGC TGCTCCTCAA ACTCCTTCTG CTGCTCCTCC TGTTGCTGCT GCTCGCCCTG 
TTTCTCTACT CTCTGCCTGG CTACCTAGCC AAGAAGATCC TGGGGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_013116034.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012971488.1 Mesocricetus auratus otoferlin (Otof), transcript variant X3, mRNA.

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CloneID	OMe127222
Clone ID Related Accession (Same CDS sequence)	XM_013116034.1
Accession Version	XM_013116034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	otoferlin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAATCGGC CCGACACGAG CTTCATCTGG 
TTCTTGAACC CTCTCAAGTC TGCCCGCTAC TTCCTGTGGC ACACCTACCG CTGGCTGCTC 
CTCAAACTCC TTCTGCTGCT CCTCCTGTTG CTGCTGCTCG CCCTGTTTCT CTACTCTCTG 
CCTGGCTACC TAGCCAAGAA GATCCTGGGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971488.1
CDS	1..5976
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRSKGREKTKGGRDGERKAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	XM_013116034.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116034.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAATCGGC CCGACACGAG CTTCATCTGG 
TTCTTGAACC CTCTCAAGTC TGCCCGCTAC TTCCTGTGGC ACACCTACCG CTGGCTGCTC 
CTCAAACTCC TTCTGCTGCT CCTCCTGTTG CTGCTGCTCG CCCTGTTTCT CTACTCTCTG 
CCTGGCTACC TAGCCAAGAA GATCCTGGGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_013116035.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012971489.1 Mesocricetus auratus otoferlin (Otof), transcript variant X2, mRNA.

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CloneID	OMe127223
Clone ID Related Accession (Same CDS sequence)	XM_013116035.1
Accession Version	XM_013116035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA TCGGCCCGAC 
ACGAGCTTCA TCTGGTTCTT GAACCCTCTC AAGTCTGCCC GCTACTTCCT GTGGCACACC 
TACCGCTGGC TGCTCCTCAA ACTCCTTCTG CTGCTCCTCC TGTTGCTGCT GCTCGCCCTG 
TTTCTCTACT CTCTGCCTGG CTACCTAGCC AAGAAGATCC TGGGGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971489.1
CDS	1..5931
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	XM_013116035.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116035.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA TCGGCCCGAC 
ACGAGCTTCA TCTGGTTCTT GAACCCTCTC AAGTCTGCCC GCTACTTCCT GTGGCACACC 
TACCGCTGGC TGCTCCTCAA ACTCCTTCTG CTGCTCCTCC TGTTGCTGCT GCTCGCCCTG 
TTTCTCTACT CTCTGCCTGG CTACCTAGCC AAGAAGATCC TGGGGGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_013116036.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012971490.1 Mesocricetus auratus otoferlin (Otof), transcript variant X5, mRNA.

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CloneID	OMe127224
Clone ID Related Accession (Same CDS sequence)	XM_013116036.1 , XM_013116036.2
Accession Version	XM_013116036.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	otoferlin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA CCGGCCTGAC 
ACAGCCTTCG TCTGGTTCCT GAACCCGCTC AAGTCGATCA AGTACCTCAT CTGCACCCGA 
TACAAGTGGC TGATCATCAA GATCGTGCTG GCGCTGCTGG GACTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCAGG CTACATGGTC AAGAAGCTCC TAGGGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971490.1
CDS	106..6096
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTVRLLRGCHVLCNGGPSSRPTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_013116036.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116036.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA CCGGCCTGAC 
ACAGCCTTCG TCTGGTTCCT GAACCCGCTC AAGTCGATCA AGTACCTCAT CTGCACCCGA 
TACAAGTGGC TGATCATCAA GATCGTGCTG GCGCTGCTGG GACTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCAGG CTACATGGTC AAGAAGCTCC TAGGGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_013116036.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012971490.1 Mesocricetus auratus otoferlin (Otof), transcript variant X3, mRNA.

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CloneID	OMe127224
Clone ID Related Accession (Same CDS sequence)	XM_013116036.1 , XM_013116036.2
Accession Version	XM_013116036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	otoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_013116036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA CCGGCCTGAC 
ACAGCCTTCG TCTGGTTCCT GAACCCGCTC AAGTCGATCA AGTACCTCAT CTGCACCCGA 
TACAAGTGGC TGATCATCAA GATCGTGCTG GCGCTGCTGG GACTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCAGG CTACATGGTC AAGAAGCTCC TAGGGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971490.1
CDS	185..6175
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTVRLLRGCHVLCNGGPSSRPTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_013116036.2
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116036.2

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGTCAGGCT TCTCCGGGGC TGCCACGTGC TGTGCAATGG GGGCCCCTCT 
TCTCGCCCCA CAGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA CCGGCCTGAC 
ACAGCCTTCG TCTGGTTCCT GAACCCGCTC AAGTCGATCA AGTACCTCAT CTGCACCCGA 
TACAAGTGGC TGATCATCAA GATCGTGCTG GCGCTGCTGG GACTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCAGG CTACATGGTC AAGAAGCTCC TAGGGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_005073511.2 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005073568.1 Mesocricetus auratus otoferlin (Otof), transcript variant X6, mRNA.

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CloneID	OMe127225
Clone ID Related Accession (Same CDS sequence)	XM_005073511.2 , XM_005073511.3
Accession Version	XM_005073511.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	otoferlin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 13, 2015 this sequence version replaced XM_005073511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAACCGGC CTGACACAGC CTTCGTCTGG 
TTCCTGAACC CGCTCAAGTC GATCAAGTAC CTCATCTGCA CCCGATACAA GTGGCTGATC 
ATCAAGATCG TGCTGGCGCT GCTGGGACTG CTCATGCTGG CCCTCTTCCT CTACAGCCTC 
CCAGGCTACA TGGTCAAGAA GCTCCTAGGG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005073568.1
CDS	106..6081
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRSKGREKTKGGRDGERKAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_005073511.2
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005073511.2

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAACCGGC CTGACACAGC CTTCGTCTGG 
TTCCTGAACC CGCTCAAGTC GATCAAGTAC CTCATCTGCA CCCGATACAA GTGGCTGATC 
ATCAAGATCG TGCTGGCGCT GCTGGGACTG CTCATGCTGG CCCTCTTCCT CTACAGCCTC 
CCAGGCTACA TGGTCAAGAA GCTCCTAGGG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_005073511.3 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_005073568.1 Mesocricetus auratus otoferlin (Otof), transcript variant X4, mRNA.

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CloneID	OMe127225
Clone ID Related Accession (Same CDS sequence)	XM_005073511.2 , XM_005073511.3
Accession Version	XM_005073511.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	otoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 22, 2017 this sequence version replaced XM_005073511.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAACCGGC CTGACACAGC CTTCGTCTGG 
TTCCTGAACC CGCTCAAGTC GATCAAGTAC CTCATCTGCA CCCGATACAA GTGGCTGATC 
ATCAAGATCG TGCTGGCGCT GCTGGGACTG CTCATGCTGG CCCTCTTCCT CTACAGCCTC 
CCAGGCTACA TGGTCAAGAA GCTCCTAGGG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005073568.1
CDS	185..6160
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRSKGREKTKGGRDGERKAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_005073511.3
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005073511.3

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAACCGGC CTGACACAGC CTTCGTCTGG 
TTCCTGAACC CGCTCAAGTC GATCAAGTAC CTCATCTGCA CCCGATACAA GTGGCTGATC 
ATCAAGATCG TGCTGGCGCT GCTGGGACTG CTCATGCTGG CCCTCTTCCT CTACAGCCTC 
CCAGGCTACA TGGTCAAGAA GCTCCTAGGG GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_021229223.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_021084882.1 Mesocricetus auratus otoferlin (Otof), transcript variant X5, mRNA.

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CloneID	OMe249715
Clone ID Related Accession (Same CDS sequence)	XM_021229223.1
Accession Version	XM_021229223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1495296000000
Organism	Mesocricetus auratus(golden hamster)
Product	otoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA CCGGCCTGAC 
ACAGCCTTCG TCTGGTTCCT GAACCCGCTC AAGTCGATCA AGTACCTCAT CTGCACCCGA 
TACAAGTGGC TGATCATCAA GATCGTGCTG GCGCTGCTGG GACTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCAGG CTACATGGTC AAGAAGCTCC TAGGGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021084882.1
CDS	185..6115
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_021229223.1
Organism	Mesocricetus auratus(golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021229223.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGA GCGGGAAGGA GTGTGTTCTC GGCCATGAAA 
CTTGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCAGTGCTG 
GAGATGGAGG ACCTGGATCA CCTGGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCCGTCAC CGCTCTCACC AGCAATGTCT CTAACAAGCG GTCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGAAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGTGT GGAGGTGGGC 
GATGACAAAA AATACACGTC TATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAT 
TTCGTCTTCG ACTTCCATGT CTCTCCTGAC GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTGATCCATT CTAAGAACCT GCTTCGAAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGGACCG TGTACTCCCA GCCCGAACAC CAGTTCCATC ACAAGTGGGC AATCCTGTCT 
GACCCCGACG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCTGTGGTA 
GGCAAGGGAG ACAACGTCAA GACACCCCAC AAGACCAACG AGACAGATGA GGACGACATC 
GAGGGGAACT TGCTGCTCCC CGAAGGCGTG CCCCCAGAAC GTCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCGGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTG 
AAGAAGGCTT TCATTGGTGA GAACAAGGAC CTCGTAGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAAAC GTCGGTGCAG AAGAGCAGTT ATGAGCCACT GTGGAACGAG 
CAGGTCGTCT TCACAGACCT GTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAAATCCGA 
GACTCCGACA AGGTCAACGA TGTCGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCCAACGACG GAGACAAAGG CTTCCTGCCC ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAC GAACACCAGG ACCTGAACGA GGGTCTGGGG 
GAGGGTGTGT CCTTCCGCGC CCGCCTCATG CTGGGACTGG CTGTGGAGAT CCTGGACACC 
TCCAACCCAG AGCTCACCAG CTCCACGGAA GTGCAGGTGG AGCAGGCCAC GCCCGTCTCG 
GAGAGCTGCA CAGGAAGAAT GGAAGAATTT TTTCTATTTG GAGCCTTCCT GGAAGCTTCC 
ATGATTGACC GGAAGAACGG GGACAAACCA ATTACCTTTG AAGTGACCAT AGGAAACTAT 
GGCAACGAAG TTGATGGCAT GTCACGACCC CAGCGGCCTC GGCCCCGGAA GGAGCCTGGG 
GATGAAGAGG AAGTAGACCT GATTCAGAAC TCCAGTGATG ATGAGGGTGA TGAAGGCGGA 
GACCTAGCCT CAGTGTCTTC CACCCCCCCT ATGCGGCCCC AGATCACAGA CAGGAACTAC 
TTTCACCTGC CCTACCTGGA GCGCAAGCCC TGCATCTACA TTAAGAGCTG GTGGCCTGAT 
CAGCGACGAC GCCTCTACAA TGCAAACATC ATGGACCACA TAGCTGATAA GCTGGAAGAA 
GGTCTGAATG ACGTGCAGGA GATGATCAAA ACAGAGAAGT CCTACCCGGA GCGCCGCCTG 
CGGGGCGTGC TAGAGGAACT TAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGCC ACGCGTCCCG CACCAAGCTG GATCGGGAGC GCCTCAAGTC CTGTATGAGG 
GAGCTGGAGA GCATGGGGCA GCAGGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTGCGGGACA AGCTGAGGCT ATGCCAGAAC TTCCTACAGA AGCTACGCTT CCTGGCAGAT 
GAGCCCCAGC ACAGCATTCC AGACGTGTTC ATTTGGATGA TGAGCAACAA CAAGCGTATC 
GCCTACGCCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TTGTGGAGGA GGAGATGGGC 
AAGGACTGCG CCAAAGTCAA GACCCTCTTC CTCAAGCTGC CAGGGAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG CCTCAGTAAG 
CAGCGAAAGG ACTTCCTGTG CGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCACTCCTT TCCACCCATC AGCCTGGTCT ACACCAAGAA GCAAGCCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTTG CTGCCGACAG CAGTGGGCTC 
TCTGATCCCT TCGCCCGTGT CTTCTTCATC AACCAAAGCC AGTGCACAGA GGTTCTGAAT 
GAGACGCTGT GTCCCACTTG GGACCAAATG CTCGTATTTG ACAACCTGGA GCTGTATGGT 
GAAGCGCATG AATTACGAGA CGATCCCCCC ATCATTGTCA TTGAAATTTA CGACCAGGAC 
ACCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AGCCCCTGGT GAAGATGGCA 
GAGGAGGCAT ACTGCCCGCC TCGCTTCCCG CCCCAGCTCG AGTACTACCA GATCTACCGC 
GGCAATGCCA CTGCCGGGGA CCTCCTGGCT GCCTTCGAGC TGCTGCAGAT TGGGCCATCC 
GGGAAGGCCG ACCTGCCACC CATCAACGGC CCGGTGGACA TGGACAGAGG ACCCATCATG 
CCTGTGCCAG TGGGAATCCG GCCAGTGCTC AGCAAGTATC GAGTAGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTGAAGAG GGTGAACCTG GCCCAGGTGG ACCGACCACG GGTGGACATC 
GAGTGTGCGG GGAAAGGGGT GCAGTCATCC CTGATCCACA ACTATAAGAA GAGCCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGAGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCTGTCA GCTCCCTGCG ACGCTTCATC TACAGACCCC CGGACCGCAC AGCCCCCAAC 
TGGAACACCA CCGGGGAGGT GGTAGTGAGC ATGGAGCCCG AGGTGCCTGT CAAGAAGCTG 
GAGACCATGG TGAAGCTGGA TGCGACTTCT GATGCCGTGG TCAAGGTGGA CGTGGCTGAG 
GAGGAGAAGG AAAGGAAGAA GAGGAAAAAG AAAGGAGCGT CAGACGAGGC AGAGGAAGAG 
GAGCCGGATG AGAGCATGCT GGACTGGTGG TCCAAGTACT TTGCCTCCAT CGACACAATG 
AAGGAGCAAC TTCGGCAACA TGAGGCCTCT GGAATTGACT TAGAAGAGAA GGAAGAAATG 
GATACCACTG AGGGCCTGAA GGGGCCGATA AAGAACAAGG ATAAATCCAG GGCTGCCAAG 
GAGGAGAAGA AGAAGAAAAT CCCTGGTCCT GGCCAGGGAC CAGAGGCTCC TGAGAAGAAG 
AAAGCCAAGA TCGACGAGCT GAAGGTGTAC CCCAAAGAGC TGGAAACAGA GTTCGACAAC 
TTTGAGGACT GGCTGCATAC CTTCAACCTA TTGCGGGGCA AGACGGGGGA TGATGAAGAT 
GGGTCCACAG AGGAAGAACG AATCGTGGGT CGGTTCAAGG GTTCCCTCTG TGTGTACAAA 
GTGCCACTCC CAGAGGATGT ATCTCGAGAA GCTGGCTATG ACCCCACCTA TGGAATGTTC 
CAAGGCATCC CAAGCAATGA CCCTATTAAT GTGCTGGTTC GAATCTATGT GGTCCGGGCC 
ACAGACCTGC ACCCGGCTGA CATCAATGGC AAAGCTGACC CCTATATCGC CATCAAGCTA 
GGCAAGACTG ACATCCGGGA CAAGGAGAAC TACATCTCCA AGCAGCTCAA CCCCGTCTTT 
GGGAAGTCCT TTGACATCGA GGCCTCCTTC CCAATGGAGT CCATGTTGAC AGTGGCCGTG 
TATGACTGGG ATCTGGTGGG CACTGATGAC CTCATTGGAG AAACCAAGAT CGACCTGGAA 
AACCGCTTCT ACAGCAAGCA CCGGGCTACC TGCGGCATTG CGCAGACCTA CTCCATACAC 
GGCTACAATA TCTGGAGGGA CCCTATGAAG CCCAGTCAGA TCCTAACCCG TCTCTGCAAA 
GAAGGCAAAG TGGACGGCCC CCACTTTGGT CCCCATGGGA GAGTGAAGGT CGCCAACCGT 
GTCTTCACGG GGCCTTCAGA GATAGAGGAC GAGAATGGTC AGAAGAAGCC TACAGATGAG 
CACGTGGCAT TGTCTGCTCT GAGACACTGG GAGGACATCC CCCGGGTGGG CTGCCGCCTT 
GTACCAGAAC ACGTGGAGAC CAGGCCGCTG CTCAACCCTG ACAAGCCAGG CATCGAGCAG 
GGTCGCCTGG AGCTGTGGGT GGATATGTTC CCCATGGACA TGCCAGCTCC TGGGACGCCT 
CTGGATATCT CCCCCAGGAA ACCCAAGAAG TACGAGCTGC GGGTCATCGT GTGGAACACA 
GATGAGGTGG TCCTAGAAGA TGACGATTTC TTCACAGGAG AGAAGTCCAG TGACATTTTT 
GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG GACAAACAGG ACACAGACGT CCACTACCAC 
TCCCTCACTG GTGAAGGCAA CTTTAACTGG CGTTACCTGT TCCCCTTCGA CTACCTGGCT 
GCCGAAGAGA GGATTGTTAT GTCTAAGAAG GAGTCTATGT TCTCCTGGGA TGAGACGGAG 
TACAAGATCC CTGCACGGCT CACCTTGCAG ATCTGGGATG CTGACCACTT CTCTGCTGAC 
GACTTCCTGG GGGCCATTGA GCTGGACCTG AACCGGTTTC CAAGGGGCGC TAAGACAGCC 
AAGCAGTGTA CCATGGAGAT GGTCACTGGG GAGGTGGACG TGCCCCTGGT TTCCATCTTT 
AAACAGAAGC GCGTCAAAGG CTGGTGGCCC CTCCTGGCCC GCAATGAGAA TGATGAGTTT 
GAGCTCACGG GCAAGGTGGA GGCAGAGCTA CACTTATTAA CAGCAGAGGA GGCAGAGAAG 
AATCCCGTGG GCCTGGCCCG CAATGAACCT GACCCTCTAG AAAAACCCAA CCGGCCTGAC 
ACAGCCTTCG TCTGGTTCCT GAACCCGCTC AAGTCGATCA AGTACCTCAT CTGCACCCGA 
TACAAGTGGC TGATCATCAA GATCGTGCTG GCGCTGCTGG GACTGCTCAT GCTGGCCCTC 
TTCCTCTACA GCCTCCCAGG CTACATGGTC AAGAAGCTCC TAGGGGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_013116032.1 ) cDNA ORF clone, Mesocricetus auratus(golden hamster) -> XP_012971486.1 Mesocricetus auratus otoferlin (Otof), transcript variant X1, mRNA.

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CloneID	OMe127220
Clone ID Related Accession (Same CDS sequence)	XM_013116032.1
Accession Version	XM_013116032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436630400000
Organism	Mesocricetus auratus(Golden hamster)
Product	otoferlin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004801644.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesocricetus auratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGTC 
AGGCTTCTCC GGGGCTGCCA CGTGCTGTGC AATGGGGGCC CCTCTTCTCG CCCCACAGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAATCGGC CCGACACGAG CTTCATCTGG 
TTCTTGAACC CTCTCAAGTC TGCCCGCTAC TTCCTGTGGC ACACCTACCG CTGGCTGCTC 
CTCAAACTCC TTCTGCTGCT CCTCCTGTTG CTGCTGCTCG CCCTGTTTCT CTACTCTCTG 
CCTGGCTACC TAGCCAAGAA GATCCTGGGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012971486.1
CDS	106..6141
Translation	MALIVHLKTVSELRGRSDRIAKVTFRGQSFYSRVLENCENVADFDETFRWPVASNIDRNEMLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVDVTDTLIDDNNAIIKTSLSMEVRYQAADGTVGSWDDGDFLGDEALQEEEKDSQETDGLLPGSRPSTRTPGEKSFRSKGREKTKGGRDGERKAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNVKTPHKTNETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPITFEVTIGNYGNEVDGMSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEGGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHASRTKLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEEMGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDTMGKADFMGRTFAKPLVKMAEEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKSPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRTAPNWNTTVRLLRGCHVLCNGGPSSRPTGEVVVSMEPEVPVKKLETMVKLDATSDAVVKVDVAEEEKERKKRKKKGASDEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHEASGIDLEEKEEMDTTEGLKGPIKNKDKSRAAKEEKKKKIPGPGQGPEAPEKKKAKIDELKVYPKELETEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQKKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEERIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMVTGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	XM_013116032.1
Organism	Mesocricetus auratus(Golden hamster)
Definition	Mesocricetus auratus otoferlin (Otof), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013116032.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACCGTC TCGGAGCTGC GAGGCAGAAG TGACCGGATC 
GCCAAAGTAA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAA CTGTGAAAAT 
GTGGCCGACT TCGATGAGAC GTTCCGGTGG CCGGTGGCCA GCAACATCGA CAGAAATGAA 
ATGTTGGAGA TTCAAATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT CATTGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGTG TAGACGTGAC TGACACACTG 
ATCGATGACA ACAATGCCAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAGGCC 
GCAGACGGCA CAGTGGGCTC CTGGGATGAT GGAGACTTCC TGGGAGATGA AGCTCTTCAG 
GAGGAAGAGA AGGACAGCCA GGAGACTGAT GGGCTGCTCC CTGGTTCGCG GCCCAGCACC 
AGAACACCCG GCGAGAAGAG CTTCCGCAGC AAAGGCAGAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC GCAAAGCGGG AAGGAGTGTG TTCTCGGCCA TGAAACTTGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCAG TGCTGGAGAT GGAGGACCTG 
GATCACCTGG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCC 
GTCACCGCTC TCACCAGCAA TGTCTCTAAC AAGCGGTCTA AGCCAGACAT TAAGATGGAG 
CCAAGTGCTG GAAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATCGA GGCTCGGCAG 
CTGGTGGGCT TGAACATGGA CCCTGTGGTG TGTGTGGAGG TGGGCGATGA CAAAAAATAC 
ACGTCTATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTATTTCGT CTTCGACTTC 
CATGTCTCTC CTGACGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTGAT CCATTCTAAG 
AACCTGCTTC GAAGTGGCAC CCTGGTGGGC TCCTTCAAAA TGGACGTGGG GACCGTGTAC 
TCCCAGCCCG AACACCAGTT CCATCACAAG TGGGCAATCC TGTCTGACCC CGACGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCTG TGGTAGGCAA GGGAGACAAC 
GTCAAGACAC CCCACAAGAC CAACGAGACA GATGAGGACG ACATCGAGGG GAACTTGCTG 
CTCCCCGAAG GCGTGCCCCC AGAACGTCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCGGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTGAAGAA GGCTTTCATT 
GGTGAGAACA AGGACCTCGT AGACCCCTAC GTGCAAGTCT TCTTTGCTGG CCAGAAGGGC 
AAAACGTCGG TGCAGAAGAG CAGTTATGAG CCACTGTGGA ACGAGCAGGT CGTCTTCACA 
GACCTGTTCC CCCCACTCTG CAAACGCATG AAGGTGCAAA TCCGAGACTC CGACAAGGTC 
AACGATGTCG CCATCGGCAC CCACTTCATT GACCTGCGCA AGATTTCCAA CGACGGAGAC 
AAAGGCTTCC TGCCCACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGTAAC 
TACACGCTGC TGGACGAACA CCAGGACCTG AACGAGGGTC TGGGGGAGGG TGTGTCCTTC 
CGCGCCCGCC TCATGCTGGG ACTGGCTGTG GAGATCCTGG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CGGAAGTGCA GGTGGAGCAG GCCACGCCCG TCTCGGAGAG CTGCACAGGA 
AGAATGGAAG AATTTTTTCT ATTTGGAGCC TTCCTGGAAG CTTCCATGAT TGACCGGAAG 
AACGGGGACA AACCAATTAC CTTTGAAGTG ACCATAGGAA ACTATGGCAA CGAAGTTGAT 
GGCATGTCAC GACCCCAGCG GCCTCGGCCC CGGAAGGAGC CTGGGGATGA AGAGGAAGTA 
GACCTGATTC AGAACTCCAG TGATGATGAG GGTGATGAAG GCGGAGACCT AGCCTCAGTG 
TCTTCCACCC CCCCTATGCG GCCCCAGATC ACAGACAGGA ACTACTTTCA CCTGCCCTAC 
CTGGAGCGCA AGCCCTGCAT CTACATTAAG AGCTGGTGGC CTGATCAGCG ACGACGCCTC 
TACAATGCAA ACATCATGGA CCACATAGCT GATAAGCTGG AAGAAGGTCT GAATGACGTG 
CAGGAGATGA TCAAAACAGA GAAGTCCTAC CCGGAGCGCC GCCTGCGGGG CGTGCTAGAG 
GAACTTAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGCCACGCG 
TCCCGCACCA AGCTGGATCG GGAGCGCCTC AAGTCCTGTA TGAGGGAGCT GGAGAGCATG 
GGGCAGCAGG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTGCG GGACAAGCTG 
AGGCTATGCC AGAACTTCCT ACAGAAGCTA CGCTTCCTGG CAGATGAGCC CCAGCACAGC 
ATTCCAGACG TGTTCATTTG GATGATGAGC AACAACAAGC GTATCGCCTA CGCCCGGGTG 
CCCTCCAAGG ACCTGCTCTT CTCCATTGTG GAGGAGGAGA TGGGCAAGGA CTGCGCCAAA 
GTCAAGACCC TCTTCCTCAA GCTGCCAGGG AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAGCT CTACCTGTGG CTGGGCCTCA GTAAGCAGCG AAAGGACTTC 
CTGTGCGGCC TGCCCTGCGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAC 
TCCTTTCCAC CCATCAGCCT GGTCTACACC AAGAAGCAAG CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTTGCTGCC GACAGCAGTG GGCTCTCTGA TCCCTTCGCC 
CGTGTCTTCT TCATCAACCA AAGCCAGTGC ACAGAGGTTC TGAATGAGAC GCTGTGTCCC 
ACTTGGGACC AAATGCTCGT ATTTGACAAC CTGGAGCTGT ATGGTGAAGC GCATGAATTA 
CGAGACGATC CCCCCATCAT TGTCATTGAA ATTTACGACC AGGACACCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT CGCCAAGCCC CTGGTGAAGA TGGCAGAGGA GGCATACTGC 
CCGCCTCGCT TCCCGCCCCA GCTCGAGTAC TACCAGATCT ACCGCGGCAA TGCCACTGCC 
GGGGACCTCC TGGCTGCCTT CGAGCTGCTG CAGATTGGGC CATCCGGGAA GGCCGACCTG 
CCACCCATCA ACGGCCCGGT GGACATGGAC AGAGGACCCA TCATGCCTGT GCCAGTGGGA 
ATCCGGCCAG TGCTCAGCAA GTATCGAGTA GAGGTGCTGT TCTGGGGCCT GAGGGACTTG 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGA CCACGGGTGG ACATCGAGTG TGCGGGGAAA 
GGGGTGCAGT CATCCCTGAT CCACAACTAT AAGAAGAGCC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGA 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC TGTCAGCTCC 
CTGCGACGCT TCATCTACAG ACCCCCGGAC CGCACAGCCC CCAACTGGAA CACCACCGTC 
AGGCTTCTCC GGGGCTGCCA CGTGCTGTGC AATGGGGGCC CCTCTTCTCG CCCCACAGGG 
GAGGTGGTAG TGAGCATGGA GCCCGAGGTG CCTGTCAAGA AGCTGGAGAC CATGGTGAAG 
CTGGATGCGA CTTCTGATGC CGTGGTCAAG GTGGACGTGG CTGAGGAGGA GAAGGAAAGG 
AAGAAGAGGA AAAAGAAAGG AGCGTCAGAC GAGGCAGAGG AAGAGGAGCC GGATGAGAGC 
ATGCTGGACT GGTGGTCCAA GTACTTTGCC TCCATCGACA CAATGAAGGA GCAACTTCGG 
CAACATGAGG CCTCTGGAAT TGACTTAGAA GAGAAGGAAG AAATGGATAC CACTGAGGGC 
CTGAAGGGGC CGATAAAGAA CAAGGATAAA TCCAGGGCTG CCAAGGAGGA GAAGAAGAAG 
AAAATCCCTG GTCCTGGCCA GGGACCAGAG GCTCCTGAGA AGAAGAAAGC CAAGATCGAC 
GAGCTGAAGG TGTACCCCAA AGAGCTGGAA ACAGAGTTCG ACAACTTTGA GGACTGGCTG 
CATACCTTCA ACCTATTGCG GGGCAAGACG GGGGATGATG AAGATGGGTC CACAGAGGAA 
GAACGAATCG TGGGTCGGTT CAAGGGTTCC CTCTGTGTGT ACAAAGTGCC ACTCCCAGAG 
GATGTATCTC GAGAAGCTGG CTATGACCCC ACCTATGGAA TGTTCCAAGG CATCCCAAGC 
AATGACCCTA TTAATGTGCT GGTTCGAATC TATGTGGTCC GGGCCACAGA CCTGCACCCG 
GCTGACATCA ATGGCAAAGC TGACCCCTAT ATCGCCATCA AGCTAGGCAA GACTGACATC 
CGGGACAAGG AGAACTACAT CTCCAAGCAG CTCAACCCCG TCTTTGGGAA GTCCTTTGAC 
ATCGAGGCCT CCTTCCCAAT GGAGTCCATG TTGACAGTGG CCGTGTATGA CTGGGATCTG 
GTGGGCACTG ATGACCTCAT TGGAGAAACC AAGATCGACC TGGAAAACCG CTTCTACAGC 
AAGCACCGGG CTACCTGCGG CATTGCGCAG ACCTACTCCA TACACGGCTA CAATATCTGG 
AGGGACCCTA TGAAGCCCAG TCAGATCCTA ACCCGTCTCT GCAAAGAAGG CAAAGTGGAC 
GGCCCCCACT TTGGTCCCCA TGGGAGAGTG AAGGTCGCCA ACCGTGTCTT CACGGGGCCT 
TCAGAGATAG AGGACGAGAA TGGTCAGAAG AAGCCTACAG ATGAGCACGT GGCATTGTCT 
GCTCTGAGAC ACTGGGAGGA CATCCCCCGG GTGGGCTGCC GCCTTGTACC AGAACACGTG 
GAGACCAGGC CGCTGCTCAA CCCTGACAAG CCAGGCATCG AGCAGGGTCG CCTGGAGCTG 
TGGGTGGATA TGTTCCCCAT GGACATGCCA GCTCCTGGGA CGCCTCTGGA TATCTCCCCC 
AGGAAACCCA AGAAGTACGA GCTGCGGGTC ATCGTGTGGA ACACAGATGA GGTGGTCCTA 
GAAGATGACG ATTTCTTCAC AGGAGAGAAG TCCAGTGACA TTTTTGTGCG GGGGTGGCTG 
AAGGGCCAGC AGGAGGACAA ACAGGACACA GACGTCCACT ACCACTCCCT CACTGGTGAA 
GGCAACTTTA ACTGGCGTTA CCTGTTCCCC TTCGACTACC TGGCTGCCGA AGAGAGGATT 
GTTATGTCTA AGAAGGAGTC TATGTTCTCC TGGGATGAGA CGGAGTACAA GATCCCTGCA 
CGGCTCACCT TGCAGATCTG GGATGCTGAC CACTTCTCTG CTGACGACTT CCTGGGGGCC 
ATTGAGCTGG ACCTGAACCG GTTTCCAAGG GGCGCTAAGA CAGCCAAGCA GTGTACCATG 
GAGATGGTCA CTGGGGAGGT GGACGTGCCC CTGGTTTCCA TCTTTAAACA GAAGCGCGTC 
AAAGGCTGGT GGCCCCTCCT GGCCCGCAAT GAGAATGATG AGTTTGAGCT CACGGGCAAG 
GTGGAGGCAG AGCTACACTT ATTAACAGCA GAGGAGGCAG AGAAGAATCC CGTGGGCCTG 
GCCCGCAATG AACCTGACCC TCTAGAAAAA CCCAATCGGC CCGACACGAG CTTCATCTGG 
TTCTTGAACC CTCTCAAGTC TGCCCGCTAC TTCCTGTGGC ACACCTACCG CTGGCTGCTC 
CTCAAACTCC TTCTGCTGCT CCTCCTGTTG CTGCTGCTCG CCCTGTTTCT CTACTCTCTG 
CCTGGCTACC TAGCCAAGAA GATCCTGGGG GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Otof
Entrez Gene ID	101837431
Full Name	otoferlin
Gene Type	protein-coding
Organism	Mesocricetus auratus(golden hamster)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Otof cDNA ORF clone, Mesocricetus auratus(golden hamster)

Gene

Clones

gRNAs

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