med23 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	med23
Entrez Gene ID	101482184
Full Name	mediator complex subunit 23
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004542021.2	XP_004542078.1	mediator of RNA polymerase II transcription subunit 23 isoform X2
XM_004542021.4	XP_004542078.1	mediator of RNA polymerase II transcription subunit 23 isoform X2
XM_023155146.1	XP_023010914.1	mediator of RNA polymerase II transcription subunit 23 isoform X3
XM_004542020.3	XP_004542077.1	mediator of RNA polymerase II transcription subunit 23 isoform X1
XM_004542020.4	XP_004542077.1	mediator of RNA polymerase II transcription subunit 23 isoform X1
XM_023155146.2	XP_023010914.1	mediator of RNA polymerase II transcription subunit 23 isoform X3
XM_004542020.2	XP_004542077.1	mediator of RNA polymerase II transcription subunit 23 isoform X1
XM_004542021.3	XP_004542078.1	mediator of RNA polymerase II transcription subunit 23 isoform X2

Pathways from BioSystems
Gene Ontology

The following med23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa24768	XM_004542020.4 Latest version!	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa24769	XM_004542021.4 Latest version!	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa311625	XM_023155146.2 Latest version!	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OMa24769	XM_004542021.2	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa24768	XM_004542020.3	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa311625	XM_023155146.1	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa24768	XM_004542020.2	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa24769	XM_004542021.3	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med23 ( XM_004542020.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004542077.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa24768
Clone ID Related Accession (Same CDS sequence)	XM_004542020.3 , XM_004542020.4 , XM_004542020.2
Accession Version	XM_004542020.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447372.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004542020.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004542077.1
CDS	154..4356
Translation	MALSMETQLQSIFEDVVKTEMIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQTLNIAQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_004542020.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004542020.3

ORF Insert Sequence:

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_004542020.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004542077.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa24768
Clone ID Related Accession (Same CDS sequence)	XM_004542020.3 , XM_004542020.4 , XM_004542020.2
Accession Version	XM_004542020.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036794.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004542020.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004542077.1
CDS	149..4351
Translation	MALSMETQLQSIFEDVVKTEMIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQTLNIAQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_004542020.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004542020.4

ORF Insert Sequence:

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_004542020.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004542077.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.

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CloneID	OMa24768
Clone ID Related Accession (Same CDS sequence)	XM_004542020.3 , XM_004542020.4 , XM_004542020.2
Accession Version	XM_004542020.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447372.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004542020.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004542077.1
CDS	137..4339
Translation	MALSMETQLQSIFEDVVKTEMIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQTLNIAQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_004542020.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004542020.2

ORF Insert Sequence:

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_004542021.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004542078.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa24769
Clone ID Related Accession (Same CDS sequence)	XM_004542021.2 , XM_004542021.4 , XM_004542021.3
Accession Version	XM_004542021.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447372.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004542021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGCA TAAGCAGCGT 
TGCCCGGTGC TGGAAGAGCA GCTGGTGGAC CTGGTGGTGT ACGCCATGGA GCGCTCTGAG 
ACTGAGGAAC ACTTTGACGC CGATATCGGA GGAACTAGTC AGCTGCTGTG GCAGCACCTC 
TCCTCCCAGC TCATCTTCTT TGTGTTGTTT CAGTTTGCAA GTTTCCCTCA CATGGTGCTG 
TCGCTACACC AAAAGCTTGC AGGTAGAGGT CTCATTAAAG GGAGAGATCA TCTTATGTGG 
GTCCTGCTTC AGTTCATATC AGGAAGCATT CAGAAGAACG CCTTAGCTGA CTTCCTGCCT 
GTCATGAAGC TTTTTGACCT GCTTTACCCA GAAAAAGAGT GCATTCCAGT TCCTGACATT 
AACAAACCCC AGTCGACCCA TTCGTTCGCT ATGACCTGCA TCTGGATCCA TTTGAACCGC 
AAAGCTCAGA ACGACAACTC CAAACTGCAG ATACCCATAC CGCACTCTCT GAAGCTGCAC 
CATGAATTTC TTCAGCAGTC GCTGCGTAAC AAGACCCTGG GCATGTCCGA CTATAAGATC 
GCCCTGCTGT GTAACGCCTA TAGCACCAAC TCGGAGTGCT TCACTCTGCC AATGGGCGTC 
CTGGTTGAGA CCATCTATGG AAATGGATCC ATGAGGATCA ACCTTCCCGG CACCAACTGT 
ATGGCTTCGG GATCGGTCAC TCCGCTGCCC ATGAATCTGC TGGATTCGCT CACTGTGCAT 
GCAAAGATGA GTCTGATCCA CAGTATTGCC ACGCGTGTCA TCAAATTGGC CCATGCCAAG 
TCCAGCATTG CTCTTGCCCC TGCATTGGTA GAGACGTACA GCAGATTGTT GGTCTACATG 
GAAATTGAGT CCCTGGGCAT CAAAGGATTC ATCAGTCAGT TGTTGCCCAA CGTGTTCAAG 
TCTCACGCGT GGGGAATCCT TCACACATTA CTGGAGATGT TCAGTTACAG GATGCATCAC 
ATCCAGCCAC ACTACAGAGT CCAGCTGCTG AGTCACCTGC ACAGCTTGGC TGCTGTGCCA 
CAGACCAACC AGAACCAGCT ACATCTGTGT GTTGAGAGCA CAGCTCTGCG CCTGATCACA 
GCTTTGGGGA GCTCGGAGGT GCAGCCGCAG TTCACCCGCT TCCTCAATGA CCCAAAGACA 
GTTTTGTCAG CCGAATCCGA AGAGCTGAAT CGCGCCCTGA TTCTCACTCT GGCCAGAGCC 
ACACACGTCA CCGATTTCTT CACAGGGTCT GACTCCATCC AGGGCACTTG GTGTAAGGAC 
ATCCTGCAGA CAATCATGAG TTTTACGCCT CATAACTGGG CCTCACACAC GCTATCCTGC 
TTCCCTGCAC CACTACAGGC GTTTTTCAAG CAGAACAACG TTCCTCAGGA GTCGCGCTTC 
AATCTGAAGA AGAACGTCGA GGAGGAGTAC AGGAAGTGGA AGTCGATGGC CAATGAGAAT 
GACATCATCA CCCACTTCTC CATGCAGGGC TCACCTCCAC TGTTCCTCTG TCTGCTGTGG 
AAGATGCTGC TGGAGATGGA CCATATAAAC CAAATAGGCT TTAGGGTCTT GGAACGTATT 
GGAGCACGTG CACTGGTGGC TCACGTGCGC ACATTTGCCG ACTTCCTCGT CTATGAGTTC 
TCAACGTCAG CTGGCGGGCA GCAGCTTAAC AAGTGCATTG AAATACTCAA TGACATGGTC 
TGGAAATACA ACATAGTCAC GCTGGATAGA CTCATTCTGT GCCTGGCGAT GCGTAGCCAC 
GAGGGAAACG AGGCTCAGGT TTGCTACTTC ATAATCCAGC TGCTGCTGCT GAAGCCCAAT 
GACTTCAGAA ACCGAGTCAA CGATTTTGTC AAGGAGAATG CTCCCGAGCA CTGGCTGCAG 
AACGACTGGC ACAGCAAACA CATGAGCTAC CACAAGAAAT ACCCAGAGAA GTTGTACTTT 
GAGGGTCTCG CAGACCAAGT CAACCCTCCC ATGCAGCTCC AGCCTCAGTA CCTGCCAATC 
TACTTTGGGA ATGTGTGTCT GCGCTTTCTG CCCGTTTTTG ACATTGTCAT CCACCGCTTC 
CTGGAGCTCC TTCCTGTCTC CAAGTCTCTG GAGACGCTGC TGGATCATCT GGGAGGGCTG 
TACAAGTTCC ACGATCGCCC TGTGACCTAC CTGTACAACA CCCTTCACTA CTATGAGAGA 
CACCTGAGAG ACAGAACCAA CCTCAAGAGA AAACTGGTTC ATGCCATCAT GTCTTCACTG 
AAGGACAACC GCACTCCAGG CTGGTGTCTC AGCGAAACCT ATCTGAGGTT TGGCATGAAT 
GCCCGTGAAG AAAACGTGTG GATCCCTGAT GACACCTACT ACTGCAAGCT GATTGGACGC 
CTTGTGGACA CCATGGCGGG AAAGTCACCG GGTCCTTTTC CCAACTGTGA CTGGAGGTTC 
AACGAGTTCC CCAACCCTGC GGCCCACGCC CTGCACGTCA CCTGTGTGGA GCTGATGGCT 
TTGGCCGTGC CAGGAAAAGA TGTGGGAAAC GCTTTGCTTA ATGTTGTGTT GAAAAGTCAG 
CCTCTGGTCC CCAGAGAGAA CATCACTGCC TGGATGAATG CCATTGGACT TGTGATCACT 
GCACTTCCAG AACCATACTG GATCGTTCTC CACGACCGCA TCGTTTCTGT GATCAGCTCT 
CCGGCATTAA CATCGGAGAC AGAGTGGACA GGCTATCCCT TTGCCTTGCT TGACTTCACC 
GCCTGCCACC AGAGCTACAG TGAAATGAAC TGCAGCTACG TGCTAGCATT AGCACACGCC 
GTGTGGCATC ACTCATCCAT CGGACAGCTG TCTCTGATCC CCAAATTCCT GTCAGAGACT 
CTGAAGCCAG TCGTCAAGAC AGAGTTCCAG CTGCTGTACG TGTATCACCT GGTGGGGCCG 
TTCCTTCAGC GCTTCCAGCA AGAGAGGACG CGTTGCATGT TGGAGATTGG TGTTGCCTTC 
TATGAGATGC TGCAGGCAGT TGACAAACAC TGTCAGCATC TCAGCTACAT GGACCCGATC 
TGCGATTTCC TGTATCACAT TAAATACATG TACACTGGAG ACAGCGTGAA GGAGCAGGTG 
GAGAAAATCA TCATGACTCT GCGTCCTGCC ATGAAGCTGC GTCTGCGCTT CATCACGCAC 
AGCAGCAAGA TAGAGACGTC ATCCTCAGCT GCCAGCGCTG CCTCCGCTTC CTCCGCCTCT 
TCCTCCTCCG CCACCCCGCA GCCCCCTCCG TCCACACTCT CATCCTCCAC CGCTGCCGCC 
TCGCCCTCCC CCTCCTCCTC ACAGCACACA CACACACCTA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004542078.1
CDS	137..4321
Translation	MALSMETQLQSIFEDVVKTEMIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_004542021.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004542021.2

ORF Insert Sequence:

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGCA TAAGCAGCGT 
TGCCCGGTGC TGGAAGAGCA GCTGGTGGAC CTGGTGGTGT ACGCCATGGA GCGCTCTGAG 
ACTGAGGAAC ACTTTGACGC CGATATCGGA GGAACTAGTC AGCTGCTGTG GCAGCACCTC 
TCCTCCCAGC TCATCTTCTT TGTGTTGTTT CAGTTTGCAA GTTTCCCTCA CATGGTGCTG 
TCGCTACACC AAAAGCTTGC AGGTAGAGGT CTCATTAAAG GGAGAGATCA TCTTATGTGG 
GTCCTGCTTC AGTTCATATC AGGAAGCATT CAGAAGAACG CCTTAGCTGA CTTCCTGCCT 
GTCATGAAGC TTTTTGACCT GCTTTACCCA GAAAAAGAGT GCATTCCAGT TCCTGACATT 
AACAAACCCC AGTCGACCCA TTCGTTCGCT ATGACCTGCA TCTGGATCCA TTTGAACCGC 
AAAGCTCAGA ACGACAACTC CAAACTGCAG ATACCCATAC CGCACTCTCT GAAGCTGCAC 
CATGAATTTC TTCAGCAGTC GCTGCGTAAC AAGACCCTGG GCATGTCCGA CTATAAGATC 
GCCCTGCTGT GTAACGCCTA TAGCACCAAC TCGGAGTGCT TCACTCTGCC AATGGGCGTC 
CTGGTTGAGA CCATCTATGG AAATGGATCC ATGAGGATCA ACCTTCCCGG CACCAACTGT 
ATGGCTTCGG GATCGGTCAC TCCGCTGCCC ATGAATCTGC TGGATTCGCT CACTGTGCAT 
GCAAAGATGA GTCTGATCCA CAGTATTGCC ACGCGTGTCA TCAAATTGGC CCATGCCAAG 
TCCAGCATTG CTCTTGCCCC TGCATTGGTA GAGACGTACA GCAGATTGTT GGTCTACATG 
GAAATTGAGT CCCTGGGCAT CAAAGGATTC ATCAGTCAGT TGTTGCCCAA CGTGTTCAAG 
TCTCACGCGT GGGGAATCCT TCACACATTA CTGGAGATGT TCAGTTACAG GATGCATCAC 
ATCCAGCCAC ACTACAGAGT CCAGCTGCTG AGTCACCTGC ACAGCTTGGC TGCTGTGCCA 
CAGACCAACC AGAACCAGCT ACATCTGTGT GTTGAGAGCA CAGCTCTGCG CCTGATCACA 
GCTTTGGGGA GCTCGGAGGT GCAGCCGCAG TTCACCCGCT TCCTCAATGA CCCAAAGACA 
GTTTTGTCAG CCGAATCCGA AGAGCTGAAT CGCGCCCTGA TTCTCACTCT GGCCAGAGCC 
ACACACGTCA CCGATTTCTT CACAGGGTCT GACTCCATCC AGGGCACTTG GTGTAAGGAC 
ATCCTGCAGA CAATCATGAG TTTTACGCCT CATAACTGGG CCTCACACAC GCTATCCTGC 
TTCCCTGCAC CACTACAGGC GTTTTTCAAG CAGAACAACG TTCCTCAGGA GTCGCGCTTC 
AATCTGAAGA AGAACGTCGA GGAGGAGTAC AGGAAGTGGA AGTCGATGGC CAATGAGAAT 
GACATCATCA CCCACTTCTC CATGCAGGGC TCACCTCCAC TGTTCCTCTG TCTGCTGTGG 
AAGATGCTGC TGGAGATGGA CCATATAAAC CAAATAGGCT TTAGGGTCTT GGAACGTATT 
GGAGCACGTG CACTGGTGGC TCACGTGCGC ACATTTGCCG ACTTCCTCGT CTATGAGTTC 
TCAACGTCAG CTGGCGGGCA GCAGCTTAAC AAGTGCATTG AAATACTCAA TGACATGGTC 
TGGAAATACA ACATAGTCAC GCTGGATAGA CTCATTCTGT GCCTGGCGAT GCGTAGCCAC 
GAGGGAAACG AGGCTCAGGT TTGCTACTTC ATAATCCAGC TGCTGCTGCT GAAGCCCAAT 
GACTTCAGAA ACCGAGTCAA CGATTTTGTC AAGGAGAATG CTCCCGAGCA CTGGCTGCAG 
AACGACTGGC ACAGCAAACA CATGAGCTAC CACAAGAAAT ACCCAGAGAA GTTGTACTTT 
GAGGGTCTCG CAGACCAAGT CAACCCTCCC ATGCAGCTCC AGCCTCAGTA CCTGCCAATC 
TACTTTGGGA ATGTGTGTCT GCGCTTTCTG CCCGTTTTTG ACATTGTCAT CCACCGCTTC 
CTGGAGCTCC TTCCTGTCTC CAAGTCTCTG GAGACGCTGC TGGATCATCT GGGAGGGCTG 
TACAAGTTCC ACGATCGCCC TGTGACCTAC CTGTACAACA CCCTTCACTA CTATGAGAGA 
CACCTGAGAG ACAGAACCAA CCTCAAGAGA AAACTGGTTC ATGCCATCAT GTCTTCACTG 
AAGGACAACC GCACTCCAGG CTGGTGTCTC AGCGAAACCT ATCTGAGGTT TGGCATGAAT 
GCCCGTGAAG AAAACGTGTG GATCCCTGAT GACACCTACT ACTGCAAGCT GATTGGACGC 
CTTGTGGACA CCATGGCGGG AAAGTCACCG GGTCCTTTTC CCAACTGTGA CTGGAGGTTC 
AACGAGTTCC CCAACCCTGC GGCCCACGCC CTGCACGTCA CCTGTGTGGA GCTGATGGCT 
TTGGCCGTGC CAGGAAAAGA TGTGGGAAAC GCTTTGCTTA ATGTTGTGTT GAAAAGTCAG 
CCTCTGGTCC CCAGAGAGAA CATCACTGCC TGGATGAATG CCATTGGACT TGTGATCACT 
GCACTTCCAG AACCATACTG GATCGTTCTC CACGACCGCA TCGTTTCTGT GATCAGCTCT 
CCGGCATTAA CATCGGAGAC AGAGTGGACA GGCTATCCCT TTGCCTTGCT TGACTTCACC 
GCCTGCCACC AGAGCTACAG TGAAATGAAC TGCAGCTACG TGCTAGCATT AGCACACGCC 
GTGTGGCATC ACTCATCCAT CGGACAGCTG TCTCTGATCC CCAAATTCCT GTCAGAGACT 
CTGAAGCCAG TCGTCAAGAC AGAGTTCCAG CTGCTGTACG TGTATCACCT GGTGGGGCCG 
TTCCTTCAGC GCTTCCAGCA AGAGAGGACG CGTTGCATGT TGGAGATTGG TGTTGCCTTC 
TATGAGATGC TGCAGGCAGT TGACAAACAC TGTCAGCATC TCAGCTACAT GGACCCGATC 
TGCGATTTCC TGTATCACAT TAAATACATG TACACTGGAG ACAGCGTGAA GGAGCAGGTG 
GAGAAAATCA TCATGACTCT GCGTCCTGCC ATGAAGCTGC GTCTGCGCTT CATCACGCAC 
AGCAGCAAGA TAGAGACGTC ATCCTCAGCT GCCAGCGCTG CCTCCGCTTC CTCCGCCTCT 
TCCTCCTCCG CCACCCCGCA GCCCCCTCCG TCCACACTCT CATCCTCCAC CGCTGCCGCC 
TCGCCCTCCC CCTCCTCCTC ACAGCACACA CACACACCTA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_004542021.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004542078.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.

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CloneID	OMa24769
Clone ID Related Accession (Same CDS sequence)	XM_004542021.2 , XM_004542021.4 , XM_004542021.3
Accession Version	XM_004542021.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036794.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004542021.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGCA TAAGCAGCGT 
TGCCCGGTGC TGGAAGAGCA GCTGGTGGAC CTGGTGGTGT ACGCCATGGA GCGCTCTGAG 
ACTGAGGAAC ACTTTGACGC CGATATCGGA GGAACTAGTC AGCTGCTGTG GCAGCACCTC 
TCCTCCCAGC TCATCTTCTT TGTGTTGTTT CAGTTTGCAA GTTTCCCTCA CATGGTGCTG 
TCGCTACACC AAAAGCTTGC AGGTAGAGGT CTCATTAAAG GGAGAGATCA TCTTATGTGG 
GTCCTGCTTC AGTTCATATC AGGAAGCATT CAGAAGAACG CCTTAGCTGA CTTCCTGCCT 
GTCATGAAGC TTTTTGACCT GCTTTACCCA GAAAAAGAGT GCATTCCAGT TCCTGACATT 
AACAAACCCC AGTCGACCCA TTCGTTCGCT ATGACCTGCA TCTGGATCCA TTTGAACCGC 
AAAGCTCAGA ACGACAACTC CAAACTGCAG ATACCCATAC CGCACTCTCT GAAGCTGCAC 
CATGAATTTC TTCAGCAGTC GCTGCGTAAC AAGACCCTGG GCATGTCCGA CTATAAGATC 
GCCCTGCTGT GTAACGCCTA TAGCACCAAC TCGGAGTGCT TCACTCTGCC AATGGGCGTC 
CTGGTTGAGA CCATCTATGG AAATGGATCC ATGAGGATCA ACCTTCCCGG CACCAACTGT 
ATGGCTTCGG GATCGGTCAC TCCGCTGCCC ATGAATCTGC TGGATTCGCT CACTGTGCAT 
GCAAAGATGA GTCTGATCCA CAGTATTGCC ACGCGTGTCA TCAAATTGGC CCATGCCAAG 
TCCAGCATTG CTCTTGCCCC TGCATTGGTA GAGACGTACA GCAGATTGTT GGTCTACATG 
GAAATTGAGT CCCTGGGCAT CAAAGGATTC ATCAGTCAGT TGTTGCCCAA CGTGTTCAAG 
TCTCACGCGT GGGGAATCCT TCACACATTA CTGGAGATGT TCAGTTACAG GATGCATCAC 
ATCCAGCCAC ACTACAGAGT CCAGCTGCTG AGTCACCTGC ACAGCTTGGC TGCTGTGCCA 
CAGACCAACC AGAACCAGCT ACATCTGTGT GTTGAGAGCA CAGCTCTGCG CCTGATCACA 
GCTTTGGGGA GCTCGGAGGT GCAGCCGCAG TTCACCCGCT TCCTCAATGA CCCAAAGACA 
GTTTTGTCAG CCGAATCCGA AGAGCTGAAT CGCGCCCTGA TTCTCACTCT GGCCAGAGCC 
ACACACGTCA CCGATTTCTT CACAGGGTCT GACTCCATCC AGGGCACTTG GTGTAAGGAC 
ATCCTGCAGA CAATCATGAG TTTTACGCCT CATAACTGGG CCTCACACAC GCTATCCTGC 
TTCCCTGCAC CACTACAGGC GTTTTTCAAG CAGAACAACG TTCCTCAGGA GTCGCGCTTC 
AATCTGAAGA AGAACGTCGA GGAGGAGTAC AGGAAGTGGA AGTCGATGGC CAATGAGAAT 
GACATCATCA CCCACTTCTC CATGCAGGGC TCACCTCCAC TGTTCCTCTG TCTGCTGTGG 
AAGATGCTGC TGGAGATGGA CCATATAAAC CAAATAGGCT TTAGGGTCTT GGAACGTATT 
GGAGCACGTG CACTGGTGGC TCACGTGCGC ACATTTGCCG ACTTCCTCGT CTATGAGTTC 
TCAACGTCAG CTGGCGGGCA GCAGCTTAAC AAGTGCATTG AAATACTCAA TGACATGGTC 
TGGAAATACA ACATAGTCAC GCTGGATAGA CTCATTCTGT GCCTGGCGAT GCGTAGCCAC 
GAGGGAAACG AGGCTCAGGT TTGCTACTTC ATAATCCAGC TGCTGCTGCT GAAGCCCAAT 
GACTTCAGAA ACCGAGTCAA CGATTTTGTC AAGGAGAATG CTCCCGAGCA CTGGCTGCAG 
AACGACTGGC ACAGCAAACA CATGAGCTAC CACAAGAAAT ACCCAGAGAA GTTGTACTTT 
GAGGGTCTCG CAGACCAAGT CAACCCTCCC ATGCAGCTCC AGCCTCAGTA CCTGCCAATC 
TACTTTGGGA ATGTGTGTCT GCGCTTTCTG CCCGTTTTTG ACATTGTCAT CCACCGCTTC 
CTGGAGCTCC TTCCTGTCTC CAAGTCTCTG GAGACGCTGC TGGATCATCT GGGAGGGCTG 
TACAAGTTCC ACGATCGCCC TGTGACCTAC CTGTACAACA CCCTTCACTA CTATGAGAGA 
CACCTGAGAG ACAGAACCAA CCTCAAGAGA AAACTGGTTC ATGCCATCAT GTCTTCACTG 
AAGGACAACC GCACTCCAGG CTGGTGTCTC AGCGAAACCT ATCTGAGGTT TGGCATGAAT 
GCCCGTGAAG AAAACGTGTG GATCCCTGAT GACACCTACT ACTGCAAGCT GATTGGACGC 
CTTGTGGACA CCATGGCGGG AAAGTCACCG GGTCCTTTTC CCAACTGTGA CTGGAGGTTC 
AACGAGTTCC CCAACCCTGC GGCCCACGCC CTGCACGTCA CCTGTGTGGA GCTGATGGCT 
TTGGCCGTGC CAGGAAAAGA TGTGGGAAAC GCTTTGCTTA ATGTTGTGTT GAAAAGTCAG 
CCTCTGGTCC CCAGAGAGAA CATCACTGCC TGGATGAATG CCATTGGACT TGTGATCACT 
GCACTTCCAG AACCATACTG GATCGTTCTC CACGACCGCA TCGTTTCTGT GATCAGCTCT 
CCGGCATTAA CATCGGAGAC AGAGTGGACA GGCTATCCCT TTGCCTTGCT TGACTTCACC 
GCCTGCCACC AGAGCTACAG TGAAATGAAC TGCAGCTACG TGCTAGCATT AGCACACGCC 
GTGTGGCATC ACTCATCCAT CGGACAGCTG TCTCTGATCC CCAAATTCCT GTCAGAGACT 
CTGAAGCCAG TCGTCAAGAC AGAGTTCCAG CTGCTGTACG TGTATCACCT GGTGGGGCCG 
TTCCTTCAGC GCTTCCAGCA AGAGAGGACG CGTTGCATGT TGGAGATTGG TGTTGCCTTC 
TATGAGATGC TGCAGGCAGT TGACAAACAC TGTCAGCATC TCAGCTACAT GGACCCGATC 
TGCGATTTCC TGTATCACAT TAAATACATG TACACTGGAG ACAGCGTGAA GGAGCAGGTG 
GAGAAAATCA TCATGACTCT GCGTCCTGCC ATGAAGCTGC GTCTGCGCTT CATCACGCAC 
AGCAGCAAGA TAGAGACGTC ATCCTCAGCT GCCAGCGCTG CCTCCGCTTC CTCCGCCTCT 
TCCTCCTCCG CCACCCCGCA GCCCCCTCCG TCCACACTCT CATCCTCCAC CGCTGCCGCC 
TCGCCCTCCC CCTCCTCCTC ACAGCACACA CACACACCTA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004542078.1
CDS	149..4333
Translation	MALSMETQLQSIFEDVVKTEMIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_004542021.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004542021.4

ORF Insert Sequence:

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGCA TAAGCAGCGT 
TGCCCGGTGC TGGAAGAGCA GCTGGTGGAC CTGGTGGTGT ACGCCATGGA GCGCTCTGAG 
ACTGAGGAAC ACTTTGACGC CGATATCGGA GGAACTAGTC AGCTGCTGTG GCAGCACCTC 
TCCTCCCAGC TCATCTTCTT TGTGTTGTTT CAGTTTGCAA GTTTCCCTCA CATGGTGCTG 
TCGCTACACC AAAAGCTTGC AGGTAGAGGT CTCATTAAAG GGAGAGATCA TCTTATGTGG 
GTCCTGCTTC AGTTCATATC AGGAAGCATT CAGAAGAACG CCTTAGCTGA CTTCCTGCCT 
GTCATGAAGC TTTTTGACCT GCTTTACCCA GAAAAAGAGT GCATTCCAGT TCCTGACATT 
AACAAACCCC AGTCGACCCA TTCGTTCGCT ATGACCTGCA TCTGGATCCA TTTGAACCGC 
AAAGCTCAGA ACGACAACTC CAAACTGCAG ATACCCATAC CGCACTCTCT GAAGCTGCAC 
CATGAATTTC TTCAGCAGTC GCTGCGTAAC AAGACCCTGG GCATGTCCGA CTATAAGATC 
GCCCTGCTGT GTAACGCCTA TAGCACCAAC TCGGAGTGCT TCACTCTGCC AATGGGCGTC 
CTGGTTGAGA CCATCTATGG AAATGGATCC ATGAGGATCA ACCTTCCCGG CACCAACTGT 
ATGGCTTCGG GATCGGTCAC TCCGCTGCCC ATGAATCTGC TGGATTCGCT CACTGTGCAT 
GCAAAGATGA GTCTGATCCA CAGTATTGCC ACGCGTGTCA TCAAATTGGC CCATGCCAAG 
TCCAGCATTG CTCTTGCCCC TGCATTGGTA GAGACGTACA GCAGATTGTT GGTCTACATG 
GAAATTGAGT CCCTGGGCAT CAAAGGATTC ATCAGTCAGT TGTTGCCCAA CGTGTTCAAG 
TCTCACGCGT GGGGAATCCT TCACACATTA CTGGAGATGT TCAGTTACAG GATGCATCAC 
ATCCAGCCAC ACTACAGAGT CCAGCTGCTG AGTCACCTGC ACAGCTTGGC TGCTGTGCCA 
CAGACCAACC AGAACCAGCT ACATCTGTGT GTTGAGAGCA CAGCTCTGCG CCTGATCACA 
GCTTTGGGGA GCTCGGAGGT GCAGCCGCAG TTCACCCGCT TCCTCAATGA CCCAAAGACA 
GTTTTGTCAG CCGAATCCGA AGAGCTGAAT CGCGCCCTGA TTCTCACTCT GGCCAGAGCC 
ACACACGTCA CCGATTTCTT CACAGGGTCT GACTCCATCC AGGGCACTTG GTGTAAGGAC 
ATCCTGCAGA CAATCATGAG TTTTACGCCT CATAACTGGG CCTCACACAC GCTATCCTGC 
TTCCCTGCAC CACTACAGGC GTTTTTCAAG CAGAACAACG TTCCTCAGGA GTCGCGCTTC 
AATCTGAAGA AGAACGTCGA GGAGGAGTAC AGGAAGTGGA AGTCGATGGC CAATGAGAAT 
GACATCATCA CCCACTTCTC CATGCAGGGC TCACCTCCAC TGTTCCTCTG TCTGCTGTGG 
AAGATGCTGC TGGAGATGGA CCATATAAAC CAAATAGGCT TTAGGGTCTT GGAACGTATT 
GGAGCACGTG CACTGGTGGC TCACGTGCGC ACATTTGCCG ACTTCCTCGT CTATGAGTTC 
TCAACGTCAG CTGGCGGGCA GCAGCTTAAC AAGTGCATTG AAATACTCAA TGACATGGTC 
TGGAAATACA ACATAGTCAC GCTGGATAGA CTCATTCTGT GCCTGGCGAT GCGTAGCCAC 
GAGGGAAACG AGGCTCAGGT TTGCTACTTC ATAATCCAGC TGCTGCTGCT GAAGCCCAAT 
GACTTCAGAA ACCGAGTCAA CGATTTTGTC AAGGAGAATG CTCCCGAGCA CTGGCTGCAG 
AACGACTGGC ACAGCAAACA CATGAGCTAC CACAAGAAAT ACCCAGAGAA GTTGTACTTT 
GAGGGTCTCG CAGACCAAGT CAACCCTCCC ATGCAGCTCC AGCCTCAGTA CCTGCCAATC 
TACTTTGGGA ATGTGTGTCT GCGCTTTCTG CCCGTTTTTG ACATTGTCAT CCACCGCTTC 
CTGGAGCTCC TTCCTGTCTC CAAGTCTCTG GAGACGCTGC TGGATCATCT GGGAGGGCTG 
TACAAGTTCC ACGATCGCCC TGTGACCTAC CTGTACAACA CCCTTCACTA CTATGAGAGA 
CACCTGAGAG ACAGAACCAA CCTCAAGAGA AAACTGGTTC ATGCCATCAT GTCTTCACTG 
AAGGACAACC GCACTCCAGG CTGGTGTCTC AGCGAAACCT ATCTGAGGTT TGGCATGAAT 
GCCCGTGAAG AAAACGTGTG GATCCCTGAT GACACCTACT ACTGCAAGCT GATTGGACGC 
CTTGTGGACA CCATGGCGGG AAAGTCACCG GGTCCTTTTC CCAACTGTGA CTGGAGGTTC 
AACGAGTTCC CCAACCCTGC GGCCCACGCC CTGCACGTCA CCTGTGTGGA GCTGATGGCT 
TTGGCCGTGC CAGGAAAAGA TGTGGGAAAC GCTTTGCTTA ATGTTGTGTT GAAAAGTCAG 
CCTCTGGTCC CCAGAGAGAA CATCACTGCC TGGATGAATG CCATTGGACT TGTGATCACT 
GCACTTCCAG AACCATACTG GATCGTTCTC CACGACCGCA TCGTTTCTGT GATCAGCTCT 
CCGGCATTAA CATCGGAGAC AGAGTGGACA GGCTATCCCT TTGCCTTGCT TGACTTCACC 
GCCTGCCACC AGAGCTACAG TGAAATGAAC TGCAGCTACG TGCTAGCATT AGCACACGCC 
GTGTGGCATC ACTCATCCAT CGGACAGCTG TCTCTGATCC CCAAATTCCT GTCAGAGACT 
CTGAAGCCAG TCGTCAAGAC AGAGTTCCAG CTGCTGTACG TGTATCACCT GGTGGGGCCG 
TTCCTTCAGC GCTTCCAGCA AGAGAGGACG CGTTGCATGT TGGAGATTGG TGTTGCCTTC 
TATGAGATGC TGCAGGCAGT TGACAAACAC TGTCAGCATC TCAGCTACAT GGACCCGATC 
TGCGATTTCC TGTATCACAT TAAATACATG TACACTGGAG ACAGCGTGAA GGAGCAGGTG 
GAGAAAATCA TCATGACTCT GCGTCCTGCC ATGAAGCTGC GTCTGCGCTT CATCACGCAC 
AGCAGCAAGA TAGAGACGTC ATCCTCAGCT GCCAGCGCTG CCTCCGCTTC CTCCGCCTCT 
TCCTCCTCCG CCACCCCGCA GCCCCCTCCG TCCACACTCT CATCCTCCAC CGCTGCCGCC 
TCGCCCTCCC CCTCCTCCTC ACAGCACACA CACACACCTA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_004542021.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004542078.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.

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CloneID	OMa24769
Clone ID Related Accession (Same CDS sequence)	XM_004542021.2 , XM_004542021.4 , XM_004542021.3
Accession Version	XM_004542021.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447372.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 30, 2017 this sequence version replaced XM_004542021.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGCA TAAGCAGCGT 
TGCCCGGTGC TGGAAGAGCA GCTGGTGGAC CTGGTGGTGT ACGCCATGGA GCGCTCTGAG 
ACTGAGGAAC ACTTTGACGC CGATATCGGA GGAACTAGTC AGCTGCTGTG GCAGCACCTC 
TCCTCCCAGC TCATCTTCTT TGTGTTGTTT CAGTTTGCAA GTTTCCCTCA CATGGTGCTG 
TCGCTACACC AAAAGCTTGC AGGTAGAGGT CTCATTAAAG GGAGAGATCA TCTTATGTGG 
GTCCTGCTTC AGTTCATATC AGGAAGCATT CAGAAGAACG CCTTAGCTGA CTTCCTGCCT 
GTCATGAAGC TTTTTGACCT GCTTTACCCA GAAAAAGAGT GCATTCCAGT TCCTGACATT 
AACAAACCCC AGTCGACCCA TTCGTTCGCT ATGACCTGCA TCTGGATCCA TTTGAACCGC 
AAAGCTCAGA ACGACAACTC CAAACTGCAG ATACCCATAC CGCACTCTCT GAAGCTGCAC 
CATGAATTTC TTCAGCAGTC GCTGCGTAAC AAGACCCTGG GCATGTCCGA CTATAAGATC 
GCCCTGCTGT GTAACGCCTA TAGCACCAAC TCGGAGTGCT TCACTCTGCC AATGGGCGTC 
CTGGTTGAGA CCATCTATGG AAATGGATCC ATGAGGATCA ACCTTCCCGG CACCAACTGT 
ATGGCTTCGG GATCGGTCAC TCCGCTGCCC ATGAATCTGC TGGATTCGCT CACTGTGCAT 
GCAAAGATGA GTCTGATCCA CAGTATTGCC ACGCGTGTCA TCAAATTGGC CCATGCCAAG 
TCCAGCATTG CTCTTGCCCC TGCATTGGTA GAGACGTACA GCAGATTGTT GGTCTACATG 
GAAATTGAGT CCCTGGGCAT CAAAGGATTC ATCAGTCAGT TGTTGCCCAA CGTGTTCAAG 
TCTCACGCGT GGGGAATCCT TCACACATTA CTGGAGATGT TCAGTTACAG GATGCATCAC 
ATCCAGCCAC ACTACAGAGT CCAGCTGCTG AGTCACCTGC ACAGCTTGGC TGCTGTGCCA 
CAGACCAACC AGAACCAGCT ACATCTGTGT GTTGAGAGCA CAGCTCTGCG CCTGATCACA 
GCTTTGGGGA GCTCGGAGGT GCAGCCGCAG TTCACCCGCT TCCTCAATGA CCCAAAGACA 
GTTTTGTCAG CCGAATCCGA AGAGCTGAAT CGCGCCCTGA TTCTCACTCT GGCCAGAGCC 
ACACACGTCA CCGATTTCTT CACAGGGTCT GACTCCATCC AGGGCACTTG GTGTAAGGAC 
ATCCTGCAGA CAATCATGAG TTTTACGCCT CATAACTGGG CCTCACACAC GCTATCCTGC 
TTCCCTGCAC CACTACAGGC GTTTTTCAAG CAGAACAACG TTCCTCAGGA GTCGCGCTTC 
AATCTGAAGA AGAACGTCGA GGAGGAGTAC AGGAAGTGGA AGTCGATGGC CAATGAGAAT 
GACATCATCA CCCACTTCTC CATGCAGGGC TCACCTCCAC TGTTCCTCTG TCTGCTGTGG 
AAGATGCTGC TGGAGATGGA CCATATAAAC CAAATAGGCT TTAGGGTCTT GGAACGTATT 
GGAGCACGTG CACTGGTGGC TCACGTGCGC ACATTTGCCG ACTTCCTCGT CTATGAGTTC 
TCAACGTCAG CTGGCGGGCA GCAGCTTAAC AAGTGCATTG AAATACTCAA TGACATGGTC 
TGGAAATACA ACATAGTCAC GCTGGATAGA CTCATTCTGT GCCTGGCGAT GCGTAGCCAC 
GAGGGAAACG AGGCTCAGGT TTGCTACTTC ATAATCCAGC TGCTGCTGCT GAAGCCCAAT 
GACTTCAGAA ACCGAGTCAA CGATTTTGTC AAGGAGAATG CTCCCGAGCA CTGGCTGCAG 
AACGACTGGC ACAGCAAACA CATGAGCTAC CACAAGAAAT ACCCAGAGAA GTTGTACTTT 
GAGGGTCTCG CAGACCAAGT CAACCCTCCC ATGCAGCTCC AGCCTCAGTA CCTGCCAATC 
TACTTTGGGA ATGTGTGTCT GCGCTTTCTG CCCGTTTTTG ACATTGTCAT CCACCGCTTC 
CTGGAGCTCC TTCCTGTCTC CAAGTCTCTG GAGACGCTGC TGGATCATCT GGGAGGGCTG 
TACAAGTTCC ACGATCGCCC TGTGACCTAC CTGTACAACA CCCTTCACTA CTATGAGAGA 
CACCTGAGAG ACAGAACCAA CCTCAAGAGA AAACTGGTTC ATGCCATCAT GTCTTCACTG 
AAGGACAACC GCACTCCAGG CTGGTGTCTC AGCGAAACCT ATCTGAGGTT TGGCATGAAT 
GCCCGTGAAG AAAACGTGTG GATCCCTGAT GACACCTACT ACTGCAAGCT GATTGGACGC 
CTTGTGGACA CCATGGCGGG AAAGTCACCG GGTCCTTTTC CCAACTGTGA CTGGAGGTTC 
AACGAGTTCC CCAACCCTGC GGCCCACGCC CTGCACGTCA CCTGTGTGGA GCTGATGGCT 
TTGGCCGTGC CAGGAAAAGA TGTGGGAAAC GCTTTGCTTA ATGTTGTGTT GAAAAGTCAG 
CCTCTGGTCC CCAGAGAGAA CATCACTGCC TGGATGAATG CCATTGGACT TGTGATCACT 
GCACTTCCAG AACCATACTG GATCGTTCTC CACGACCGCA TCGTTTCTGT GATCAGCTCT 
CCGGCATTAA CATCGGAGAC AGAGTGGACA GGCTATCCCT TTGCCTTGCT TGACTTCACC 
GCCTGCCACC AGAGCTACAG TGAAATGAAC TGCAGCTACG TGCTAGCATT AGCACACGCC 
GTGTGGCATC ACTCATCCAT CGGACAGCTG TCTCTGATCC CCAAATTCCT GTCAGAGACT 
CTGAAGCCAG TCGTCAAGAC AGAGTTCCAG CTGCTGTACG TGTATCACCT GGTGGGGCCG 
TTCCTTCAGC GCTTCCAGCA AGAGAGGACG CGTTGCATGT TGGAGATTGG TGTTGCCTTC 
TATGAGATGC TGCAGGCAGT TGACAAACAC TGTCAGCATC TCAGCTACAT GGACCCGATC 
TGCGATTTCC TGTATCACAT TAAATACATG TACACTGGAG ACAGCGTGAA GGAGCAGGTG 
GAGAAAATCA TCATGACTCT GCGTCCTGCC ATGAAGCTGC GTCTGCGCTT CATCACGCAC 
AGCAGCAAGA TAGAGACGTC ATCCTCAGCT GCCAGCGCTG CCTCCGCTTC CTCCGCCTCT 
TCCTCCTCCG CCACCCCGCA GCCCCCTCCG TCCACACTCT CATCCTCCAC CGCTGCCGCC 
TCGCCCTCCC CCTCCTCCTC ACAGCACACA CACACACCTA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004542078.1
CDS	154..4338
Translation	MALSMETQLQSIFEDVVKTEMIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_004542021.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004542021.3

ORF Insert Sequence:

ATGGCGCTTT CGATGGAAAC ACAGCTTCAG AGCATCTTTG AAGATGTTGT GAAAACTGAG 
ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGCA TAAGCAGCGT 
TGCCCGGTGC TGGAAGAGCA GCTGGTGGAC CTGGTGGTGT ACGCCATGGA GCGCTCTGAG 
ACTGAGGAAC ACTTTGACGC CGATATCGGA GGAACTAGTC AGCTGCTGTG GCAGCACCTC 
TCCTCCCAGC TCATCTTCTT TGTGTTGTTT CAGTTTGCAA GTTTCCCTCA CATGGTGCTG 
TCGCTACACC AAAAGCTTGC AGGTAGAGGT CTCATTAAAG GGAGAGATCA TCTTATGTGG 
GTCCTGCTTC AGTTCATATC AGGAAGCATT CAGAAGAACG CCTTAGCTGA CTTCCTGCCT 
GTCATGAAGC TTTTTGACCT GCTTTACCCA GAAAAAGAGT GCATTCCAGT TCCTGACATT 
AACAAACCCC AGTCGACCCA TTCGTTCGCT ATGACCTGCA TCTGGATCCA TTTGAACCGC 
AAAGCTCAGA ACGACAACTC CAAACTGCAG ATACCCATAC CGCACTCTCT GAAGCTGCAC 
CATGAATTTC TTCAGCAGTC GCTGCGTAAC AAGACCCTGG GCATGTCCGA CTATAAGATC 
GCCCTGCTGT GTAACGCCTA TAGCACCAAC TCGGAGTGCT TCACTCTGCC AATGGGCGTC 
CTGGTTGAGA CCATCTATGG AAATGGATCC ATGAGGATCA ACCTTCCCGG CACCAACTGT 
ATGGCTTCGG GATCGGTCAC TCCGCTGCCC ATGAATCTGC TGGATTCGCT CACTGTGCAT 
GCAAAGATGA GTCTGATCCA CAGTATTGCC ACGCGTGTCA TCAAATTGGC CCATGCCAAG 
TCCAGCATTG CTCTTGCCCC TGCATTGGTA GAGACGTACA GCAGATTGTT GGTCTACATG 
GAAATTGAGT CCCTGGGCAT CAAAGGATTC ATCAGTCAGT TGTTGCCCAA CGTGTTCAAG 
TCTCACGCGT GGGGAATCCT TCACACATTA CTGGAGATGT TCAGTTACAG GATGCATCAC 
ATCCAGCCAC ACTACAGAGT CCAGCTGCTG AGTCACCTGC ACAGCTTGGC TGCTGTGCCA 
CAGACCAACC AGAACCAGCT ACATCTGTGT GTTGAGAGCA CAGCTCTGCG CCTGATCACA 
GCTTTGGGGA GCTCGGAGGT GCAGCCGCAG TTCACCCGCT TCCTCAATGA CCCAAAGACA 
GTTTTGTCAG CCGAATCCGA AGAGCTGAAT CGCGCCCTGA TTCTCACTCT GGCCAGAGCC 
ACACACGTCA CCGATTTCTT CACAGGGTCT GACTCCATCC AGGGCACTTG GTGTAAGGAC 
ATCCTGCAGA CAATCATGAG TTTTACGCCT CATAACTGGG CCTCACACAC GCTATCCTGC 
TTCCCTGCAC CACTACAGGC GTTTTTCAAG CAGAACAACG TTCCTCAGGA GTCGCGCTTC 
AATCTGAAGA AGAACGTCGA GGAGGAGTAC AGGAAGTGGA AGTCGATGGC CAATGAGAAT 
GACATCATCA CCCACTTCTC CATGCAGGGC TCACCTCCAC TGTTCCTCTG TCTGCTGTGG 
AAGATGCTGC TGGAGATGGA CCATATAAAC CAAATAGGCT TTAGGGTCTT GGAACGTATT 
GGAGCACGTG CACTGGTGGC TCACGTGCGC ACATTTGCCG ACTTCCTCGT CTATGAGTTC 
TCAACGTCAG CTGGCGGGCA GCAGCTTAAC AAGTGCATTG AAATACTCAA TGACATGGTC 
TGGAAATACA ACATAGTCAC GCTGGATAGA CTCATTCTGT GCCTGGCGAT GCGTAGCCAC 
GAGGGAAACG AGGCTCAGGT TTGCTACTTC ATAATCCAGC TGCTGCTGCT GAAGCCCAAT 
GACTTCAGAA ACCGAGTCAA CGATTTTGTC AAGGAGAATG CTCCCGAGCA CTGGCTGCAG 
AACGACTGGC ACAGCAAACA CATGAGCTAC CACAAGAAAT ACCCAGAGAA GTTGTACTTT 
GAGGGTCTCG CAGACCAAGT CAACCCTCCC ATGCAGCTCC AGCCTCAGTA CCTGCCAATC 
TACTTTGGGA ATGTGTGTCT GCGCTTTCTG CCCGTTTTTG ACATTGTCAT CCACCGCTTC 
CTGGAGCTCC TTCCTGTCTC CAAGTCTCTG GAGACGCTGC TGGATCATCT GGGAGGGCTG 
TACAAGTTCC ACGATCGCCC TGTGACCTAC CTGTACAACA CCCTTCACTA CTATGAGAGA 
CACCTGAGAG ACAGAACCAA CCTCAAGAGA AAACTGGTTC ATGCCATCAT GTCTTCACTG 
AAGGACAACC GCACTCCAGG CTGGTGTCTC AGCGAAACCT ATCTGAGGTT TGGCATGAAT 
GCCCGTGAAG AAAACGTGTG GATCCCTGAT GACACCTACT ACTGCAAGCT GATTGGACGC 
CTTGTGGACA CCATGGCGGG AAAGTCACCG GGTCCTTTTC CCAACTGTGA CTGGAGGTTC 
AACGAGTTCC CCAACCCTGC GGCCCACGCC CTGCACGTCA CCTGTGTGGA GCTGATGGCT 
TTGGCCGTGC CAGGAAAAGA TGTGGGAAAC GCTTTGCTTA ATGTTGTGTT GAAAAGTCAG 
CCTCTGGTCC CCAGAGAGAA CATCACTGCC TGGATGAATG CCATTGGACT TGTGATCACT 
GCACTTCCAG AACCATACTG GATCGTTCTC CACGACCGCA TCGTTTCTGT GATCAGCTCT 
CCGGCATTAA CATCGGAGAC AGAGTGGACA GGCTATCCCT TTGCCTTGCT TGACTTCACC 
GCCTGCCACC AGAGCTACAG TGAAATGAAC TGCAGCTACG TGCTAGCATT AGCACACGCC 
GTGTGGCATC ACTCATCCAT CGGACAGCTG TCTCTGATCC CCAAATTCCT GTCAGAGACT 
CTGAAGCCAG TCGTCAAGAC AGAGTTCCAG CTGCTGTACG TGTATCACCT GGTGGGGCCG 
TTCCTTCAGC GCTTCCAGCA AGAGAGGACG CGTTGCATGT TGGAGATTGG TGTTGCCTTC 
TATGAGATGC TGCAGGCAGT TGACAAACAC TGTCAGCATC TCAGCTACAT GGACCCGATC 
TGCGATTTCC TGTATCACAT TAAATACATG TACACTGGAG ACAGCGTGAA GGAGCAGGTG 
GAGAAAATCA TCATGACTCT GCGTCCTGCC ATGAAGCTGC GTCTGCGCTT CATCACGCAC 
AGCAGCAAGA TAGAGACGTC ATCCTCAGCT GCCAGCGCTG CCTCCGCTTC CTCCGCCTCT 
TCCTCCTCCG CCACCCCGCA GCCCCCTCCG TCCACACTCT CATCCTCCAC CGCTGCCGCC 
TCGCCCTCCC CCTCCTCCTC ACAGCACACA CACACACCTA TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_023155146.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023010914.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X3, mRNA.

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CloneID	OMa311625
Clone ID Related Accession (Same CDS sequence)	XM_023155146.1 , XM_023155146.2
Accession Version	XM_023155146.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447372.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023010914.1
CDS	104..4246
Translation	MIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQTLNIAQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_023155146.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023155146.1

ORF Insert Sequence:

ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med23 ( XM_023155146.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023010914.1 Maylandia zebra mediator complex subunit 23 (med23), transcript variant X3, mRNA.

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CloneID	OMa311625
Clone ID Related Accession (Same CDS sequence)	XM_023155146.1 , XM_023155146.2
Accession Version	XM_023155146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	mediator of RNA polymerase II transcription subunit 23 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036794.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023155146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023010914.1
CDS	118..4260
Translation	MIEEAFAGMFMDTPEDERTKLISCLGAFRQYWGTLPQESHEQCVQWIVRFIHSQHSPKRISFLYDCLAMAVETSLLPPRMVCVALITSDSLEWERTQLWALTFKLIRKIIGGVDYKGVRDLLKAVLDKIQTIPTTVSSAIVQQLLAAREVVEYILDRNACLLPAYFAITEIRKLYPEGQLSHWLLGSLISDFVDSFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPSTLRFPLRGMLPFDKDLFEPQTGLLRYVLEQPYSREMVCNMLGLNKQTLNIAQHKQRCPVLEEQLVDLVVYAMERSETEEHFDADIGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHSFAMTCIWIHLNRKAQNDNSKLQIPIPHSLKLHHEFLQQSLRNKTLGMSDYKIALLCNAYSTNSECFTLPMGVLVETIYGNGSMRINLPGTNCMASGSVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHAKSSIALAPALVETYSRLLVYMEIESLGIKGFISQLLPNVFKSHAWGILHTLLEMFSYRMHHIQPHYRVQLLSHLHSLAAVPQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMSFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMANENDIITHFSMQGSPPLFLCLLWKMLLEMDHINQIGFRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVNDFVKENAPEHWLQNDWHSKHMSYHKKYPEKLYFEGLADQVNPPMQLQPQYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYERHLRDRTNLKRKLVHAIMSSLKDNRTPGWCLSETYLRFGMNAREENVWIPDDTYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLVITALPEPYWIVLHDRIVSVISSPALTSETEWTGYPFALLDFTACHQSYSEMNCSYVLALAHAVWHHSSIGQLSLIPKFLSETLKPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMLEIGVAFYEMLQAVDKHCQHLSYMDPICDFLYHIKYMYTGDSVKEQVEKIIMTLRPAMKLRLRFITHSSKIETSSSAASAASASSASSSSATPQPPPSTLSSSTAAASPSPSSSQHTHTPM

Target ORF information:

RefSeq Version	XM_023155146.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra mediator complex subunit 23 (med23), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023155146.2

ORF Insert Sequence:

ATGATAGAAG AGGCCTTTGC TGGGATGTTT ATGGACACTC CAGAGGATGA GAGGACTAAG 
CTGATCAGTT GTCTTGGAGC CTTCAGACAG TACTGGGGAA CCCTGCCACA GGAGTCTCAT 
GAACAGTGTG TGCAATGGAT TGTGCGCTTT ATACACAGTC AGCACAGCCC CAAGAGGATC 
TCCTTCCTCT ACGACTGTCT GGCAATGGCT GTGGAGACCA GTCTTTTACC ACCCAGGATG 
GTGTGCGTGG CCTTAATAAC CTCAGACAGT TTGGAGTGGG AGAGAACACA GTTGTGGGCA 
TTAACTTTCA AGCTGATCCG CAAGATCATC GGGGGAGTGG ACTACAAGGG TGTTCGGGAC 
CTGTTGAAAG CTGTGCTGGA TAAAATTCAA ACTATTCCTA CCACTGTCAG CTCAGCTATA 
GTCCAGCAGC TTTTGGCTGC CAGAGAGGTG GTAGAATATA TCCTGGACAG AAACGCCTGT 
CTCCTTCCAG CCTACTTTGC TATCACAGAA ATTAGAAAAC TTTATCCAGA GGGGCAGCTC 
TCACACTGGC TTTTGGGCAG TTTGATATCA GACTTTGTGG ATAGTTTTAG ACCCACAGCC 
CGGATCAACT CTATCTGTGG ACGATGCAGT CTGTTGCCAG TGGTGAATAA CAGCGGTGCT 
ATCTGTAATT CCTGGAAATT AGACCCCAGC ACTCTTCGCT TCCCTCTGAG AGGCATGCTG 
CCGTTTGACA AGGACCTTTT TGAGCCACAG ACAGGGTTGC TACGCTATGT ACTAGAACAG 
CCTTATTCAA GAGAGATGGT GTGCAACATG TTGGGGCTCA ACAAGCAGAC TCTGAACATT 
GCCCAGCATA AGCAGCGTTG CCCGGTGCTG GAAGAGCAGC TGGTGGACCT GGTGGTGTAC 
GCCATGGAGC GCTCTGAGAC TGAGGAACAC TTTGACGCCG ATATCGGAGG AACTAGTCAG 
CTGCTGTGGC AGCACCTCTC CTCCCAGCTC ATCTTCTTTG TGTTGTTTCA GTTTGCAAGT 
TTCCCTCACA TGGTGCTGTC GCTACACCAA AAGCTTGCAG GTAGAGGTCT CATTAAAGGG 
AGAGATCATC TTATGTGGGT CCTGCTTCAG TTCATATCAG GAAGCATTCA GAAGAACGCC 
TTAGCTGACT TCCTGCCTGT CATGAAGCTT TTTGACCTGC TTTACCCAGA AAAAGAGTGC 
ATTCCAGTTC CTGACATTAA CAAACCCCAG TCGACCCATT CGTTCGCTAT GACCTGCATC 
TGGATCCATT TGAACCGCAA AGCTCAGAAC GACAACTCCA AACTGCAGAT ACCCATACCG 
CACTCTCTGA AGCTGCACCA TGAATTTCTT CAGCAGTCGC TGCGTAACAA GACCCTGGGC 
ATGTCCGACT ATAAGATCGC CCTGCTGTGT AACGCCTATA GCACCAACTC GGAGTGCTTC 
ACTCTGCCAA TGGGCGTCCT GGTTGAGACC ATCTATGGAA ATGGATCCAT GAGGATCAAC 
CTTCCCGGCA CCAACTGTAT GGCTTCGGGA TCGGTCACTC CGCTGCCCAT GAATCTGCTG 
GATTCGCTCA CTGTGCATGC AAAGATGAGT CTGATCCACA GTATTGCCAC GCGTGTCATC 
AAATTGGCCC ATGCCAAGTC CAGCATTGCT CTTGCCCCTG CATTGGTAGA GACGTACAGC 
AGATTGTTGG TCTACATGGA AATTGAGTCC CTGGGCATCA AAGGATTCAT CAGTCAGTTG 
TTGCCCAACG TGTTCAAGTC TCACGCGTGG GGAATCCTTC ACACATTACT GGAGATGTTC 
AGTTACAGGA TGCATCACAT CCAGCCACAC TACAGAGTCC AGCTGCTGAG TCACCTGCAC 
AGCTTGGCTG CTGTGCCACA GACCAACCAG AACCAGCTAC ATCTGTGTGT TGAGAGCACA 
GCTCTGCGCC TGATCACAGC TTTGGGGAGC TCGGAGGTGC AGCCGCAGTT CACCCGCTTC 
CTCAATGACC CAAAGACAGT TTTGTCAGCC GAATCCGAAG AGCTGAATCG CGCCCTGATT 
CTCACTCTGG CCAGAGCCAC ACACGTCACC GATTTCTTCA CAGGGTCTGA CTCCATCCAG 
GGCACTTGGT GTAAGGACAT CCTGCAGACA ATCATGAGTT TTACGCCTCA TAACTGGGCC 
TCACACACGC TATCCTGCTT CCCTGCACCA CTACAGGCGT TTTTCAAGCA GAACAACGTT 
CCTCAGGAGT CGCGCTTCAA TCTGAAGAAG AACGTCGAGG AGGAGTACAG GAAGTGGAAG 
TCGATGGCCA ATGAGAATGA CATCATCACC CACTTCTCCA TGCAGGGCTC ACCTCCACTG 
TTCCTCTGTC TGCTGTGGAA GATGCTGCTG GAGATGGACC ATATAAACCA AATAGGCTTT 
AGGGTCTTGG AACGTATTGG AGCACGTGCA CTGGTGGCTC ACGTGCGCAC ATTTGCCGAC 
TTCCTCGTCT ATGAGTTCTC AACGTCAGCT GGCGGGCAGC AGCTTAACAA GTGCATTGAA 
ATACTCAATG ACATGGTCTG GAAATACAAC ATAGTCACGC TGGATAGACT CATTCTGTGC 
CTGGCGATGC GTAGCCACGA GGGAAACGAG GCTCAGGTTT GCTACTTCAT AATCCAGCTG 
CTGCTGCTGA AGCCCAATGA CTTCAGAAAC CGAGTCAACG ATTTTGTCAA GGAGAATGCT 
CCCGAGCACT GGCTGCAGAA CGACTGGCAC AGCAAACACA TGAGCTACCA CAAGAAATAC 
CCAGAGAAGT TGTACTTTGA GGGTCTCGCA GACCAAGTCA ACCCTCCCAT GCAGCTCCAG 
CCTCAGTACC TGCCAATCTA CTTTGGGAAT GTGTGTCTGC GCTTTCTGCC CGTTTTTGAC 
ATTGTCATCC ACCGCTTCCT GGAGCTCCTT CCTGTCTCCA AGTCTCTGGA GACGCTGCTG 
GATCATCTGG GAGGGCTGTA CAAGTTCCAC GATCGCCCTG TGACCTACCT GTACAACACC 
CTTCACTACT ATGAGAGACA CCTGAGAGAC AGAACCAACC TCAAGAGAAA ACTGGTTCAT 
GCCATCATGT CTTCACTGAA GGACAACCGC ACTCCAGGCT GGTGTCTCAG CGAAACCTAT 
CTGAGGTTTG GCATGAATGC CCGTGAAGAA AACGTGTGGA TCCCTGATGA CACCTACTAC 
TGCAAGCTGA TTGGACGCCT TGTGGACACC ATGGCGGGAA AGTCACCGGG TCCTTTTCCC 
AACTGTGACT GGAGGTTCAA CGAGTTCCCC AACCCTGCGG CCCACGCCCT GCACGTCACC 
TGTGTGGAGC TGATGGCTTT GGCCGTGCCA GGAAAAGATG TGGGAAACGC TTTGCTTAAT 
GTTGTGTTGA AAAGTCAGCC TCTGGTCCCC AGAGAGAACA TCACTGCCTG GATGAATGCC 
ATTGGACTTG TGATCACTGC ACTTCCAGAA CCATACTGGA TCGTTCTCCA CGACCGCATC 
GTTTCTGTGA TCAGCTCTCC GGCATTAACA TCGGAGACAG AGTGGACAGG CTATCCCTTT 
GCCTTGCTTG ACTTCACCGC CTGCCACCAG AGCTACAGTG AAATGAACTG CAGCTACGTG 
CTAGCATTAG CACACGCCGT GTGGCATCAC TCATCCATCG GACAGCTGTC TCTGATCCCC 
AAATTCCTGT CAGAGACTCT GAAGCCAGTC GTCAAGACAG AGTTCCAGCT GCTGTACGTG 
TATCACCTGG TGGGGCCGTT CCTTCAGCGC TTCCAGCAAG AGAGGACGCG TTGCATGTTG 
GAGATTGGTG TTGCCTTCTA TGAGATGCTG CAGGCAGTTG ACAAACACTG TCAGCATCTC 
AGCTACATGG ACCCGATCTG CGATTTCCTG TATCACATTA AATACATGTA CACTGGAGAC 
AGCGTGAAGG AGCAGGTGGA GAAAATCATC ATGACTCTGC GTCCTGCCAT GAAGCTGCGT 
CTGCGCTTCA TCACGCACAG CAGCAAGATA GAGACGTCAT CCTCAGCTGC CAGCGCTGCC 
TCCGCTTCCT CCGCCTCTTC CTCCTCCGCC ACCCCGCAGC CCCCTCCGTC CACACTCTCA 
TCCTCCACCG CTGCCGCCTC GCCCTCCCCC TCCTCCTCAC AGCACACACA CACACCTATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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med23 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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