brwd3 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	brwd3
Entrez Gene ID	101476028
Full Name	bromodomain and WD repeat domain containing 3
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012925339.2	XP_012780793.1	bromodomain and WD repeat-containing protein 3
XM_023155317.2	XP_023011085.1	bromodomain and WD repeat-containing protein 3
XM_023155317.1	XP_023011085.1	bromodomain and WD repeat-containing protein 3

Pathways from BioSystems
Gene Ontology

The following brwd3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brwd3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa26199	XM_012925339.2 Latest version!	Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa311742	XM_023155317.1 Latest version!	Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa359830	XM_023155317.2 Latest version!	Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brwd3 ( XM_012925339.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012780793.1 Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.

Price & Availability↑

CloneID	OMa26199
Clone ID Related Accession (Same CDS sequence)	XM_012925339.2
Accession Version	XM_012925339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1444838400000
Organism	Maylandia zebra(Zebra mbuna)
Product	bromodomain and WD repeat-containing protein 3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447412.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_012925339.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Maylandia zebra Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAG AACAGTGTTC ACAAATCGAA GCTGAACTCT ATTACCTCAT TGCCAGATTT 
CTGCAGTCTG GACCATGTAA AAAAGCCGCA AAGATCCTGA TCGAGGAGCT GGAGGAGTAT 
GAGTTGGTCC CGCAACGATG GAGTTGGGAC GGGAAGAAAT ACAAACGGAA CTTCCAAGAT 
TGGGTGGTGT TGAACCAGCA CATTCCTGCA GACTATTTGC TCAGGATTTG CCAGCGGATA 
GGCCCACTTA TAGAAAAGGA GATCCCACCT TGTGTTCCTG GAGTCCAGAC ACTTCTGGGG 
CTGGGAAGAC AGTCCTTGCT TCGCACCAGC AAAAGTTGTA GCCACAAGGT ATGTAGTGGC 
TCAGCTGTCG CTGCACTCCA CCGAGGACGT CCACCAGAAC CTCCAGTGAG CAATGGAAGT 
TCTCCTAATG TTGTGTCCAT CATGGGTGCT CGCCAGGCCA CAGGCACAGG TCGTTTTGGC 
CAGGTTCTGC CGTCTTCCTC CTACCAACAC ATGAAGATAC ACAAACGTAT CCTTGGCCAC 
CTTTCTTCAG TCTACTGTGT GGCCTTTGAC CGTACCGGTC GTCGAATATT TACGGGTTCT 
GATGATTGCT TGGTAAAAAT CTGGGCCACA GATGATGGCC GGCTCCTGGC AACACTCCGC 
GGTCACTCAG CAGAGATCTG TGACATGGCT GTCAACCACG AGAACAGCCT CATTGCTTCT 
GCCAGCTGTG ACAAAGTCAT CAGAGTGTGG TGCTTACGCA CCTGTGCGCC CATCACTGTG 
CTGCAGGCTC ATGCTGCGTC TATCACATCC ATACAGTTTT GTCCAGCAGC TAGAGGAACA 
ACTCGGTACC TGGCATCAAC TGGTGCAGAC GGCATGGTGT GTTTCTGGAA GTGGCATTCG 
CTCACCATGA AATTTAATGA TCATCCAGTC AAGTTTATGG AGCGTTCACG GCCCGGGGTG 
CAAGTCTCCA CTTCATCTTT CAGCAGTGGT GGCATGTTCA TGGCTACTGG AGGCACTGAT 
CATATGATCA GGGTCTACTA TCTTGGTGCT GAGTCTCCTA TGAAGGTTTC TGAGATGGAT 
GCTCACACGG ATAAAGTCGT GGTCGTCCAG TTCAGCAATA ACAGTGACAG CCTAAGGTTC 
GTGAGCGGCA GTCGCGACGG TACGGTGAAG ATTTGGCATT ACCAGCAGCA GGAGTGGAAG 
AGCACCACTT TAGACATGGC TGCAAGGCTT CCAGGGAGCA CTGCCGCCAC AGGGGACGAC 
AAAGCTAAGT TGGTGGTGAC TATGGTTGCC TGGGACCGCA CTGACAGCAC CATAATCACA 
GCGGTGTCAA ACTACCTGCT CAAAGTGTGG AGCACATCCA CAGGACAGCT GCTGCACATT 
TTGTCTGGTC ATGACGACGA GGTGTTTGTG CTGGAGGCTC ATCCATTTGA CTCCCGCATC 
ATGTTATCTG CCGGCCACGA TGGCAACATT TACATCTGGG ACCTAACCAA AGGAGTCAAG 
ATCCGCAATT TCTTCAACAT GATTGAGGGC CAAGGCCACG GTGCTATTTT TGACTGCAAG 
TTCTCAGCAG ATGGGCAGCA TTTTGCCTGC ACTGACTCAC ACGGCCATTT GCTCATCTTT 
GGTTTTGGCT GCAGCAGACC ATATGAAAAG ATTCCCGACC AGATGTTCTT CCACACGGAC 
TATCGCCCTC TTATCCGTGA CTCTAATAAC TACGTCCTGG ATGAGCAGAC GCAACAGGCG 
CCACACCTCA TGCCTCCACC CTTCCTTGTG GATGTCGACG GGAATCCTCA TCCTTCTCAC 
TACCAGCGCC TGGTGCCTGG ACGGGAGAAC TGCAAGGAAG AGCAGCTAAT ACCTCAGATG 
GGATACATGG CTAACAATGA TGGAGAGTTG GTTGAGCAGG TTCTTGGCCA GCAAACGGCA 
GAAAATGGAG AAAGCCCATT GGACAATCTC ATCAGACAGC TGCAGAATGA ACAAGATGAG 
CGTCAGAATG CAGAGCGGGA GGCAGAAGGG GAAGAAGCTG GTGAAGGCGC AGAGGCGGTG 
CCCTCCTCTC CAGTTGTTGC ACCTCGCAGA AGTGGGCAGG TGGATGGGGT GTGGCAAATG 
CAGCATAACG CCCTTCCCAG TCAGGTGGCC ACCGAGAGGG ACCTGCTGGC CTGGAGCCGC 
AGAGTGGTGG TCAATGAAGT GCTGCAGGGG ATATTCAGAG TTTCAGAGGA GTGCAGACAA 
GCCAAAGGTG ACATTGAATG TTCTCTATAC TACGCTGAGA GGAAGAAGAA ACCAGTAACC 
TGTGCACCTA AGAGCGATCT CTTGTCTTCA CGCCTGCGCT CCCTGCGGCC CACGAAGAAA 
ACTCATAAGC GCCATGCCTA TCAAACCCGG TCAGCTCAAA TCCGGCGTCC TGGAACCAGG 
AGTAGAAATC CCAGCAACCG ACGTACCTCT AACAGCCAGA TGGACTCGGA GAGTGATGGA 
GAACAGGCAG AAGAACAGGT GGAGCAAACC GATGCATCTT CAGATGGTGA TGCTCGGTGG 
CAGAGTGAAA GCAGCTCCAG CGATTCCTCC AGTGAATATT CTGATTGGAC AGCGGATGCT 
GGGATCAATT TGCAGCCGCC AAAGCGACCT ACCAGGAGAC CTGTACAGCC TCCAGATTAC 
AGCAGCTCTG AGGAAGAAGG AGATAACGAA GCCAAGGAGG CAAGGACGAG GGAAAAAGAG 
AAGAGGAAGA AGAAGTCCAA GGAGACCAAA CAAAAACCCA CCTCCTCTCT GGGTGGACTT 
GATGCGGAAG AGTGGCTGCC GCCATCTTGG ATTATGGAGA CTGTCCCCCG CTGCTCTCCT 
TTTGTTCCAC AAATGGGAGA TGAACTCATC TACTTCAAAC AGGGCCATCA GGCTTACGTC 
CGGGCTGTTC GCAGAGCTAA GGCTTACAGC ATCAACCCTC AGAAGCAGCC GTGGAACAGA 
CTCAACCTCA GGGATCAGGA ATCTGTGAAA GTGGTTGGAA TTAAGTATGA AGTGGGACCT 
CCTACCCTCT GCTGCTTAAA AGTGGCGTTC TTGGACCCAG TCTCAGGGAA AATGACCAAT 
GAGTCTTTCT CCTTAAAGTA TCATGACATG CCTGATGTCA TTGATTTTCT GGTACTGCAG 
CAGTTTTACA ACGAGGCTAA AGAGCACAAC TGGCAACCTG GCATGAGGTT CCGCAGCATC 
ATTGACGACG CCTGGTGGTT CGGCTCTGTG GAGGATCAAG AGCCCCTGCA GCCAGAGTAT 
CCCGACAGCC TGTTTCAGTG CTACGCAGTA AAATGGGATA ACGGTGAGCG GGAGAAAATG 
AGTCCCTGGG ATATGGAGCC TATTCCTGAG GAAGCTGCTT TGCCAGAGCA GGTTGGTGAT 
GGTGTAGAGG TGGCAGAAGA GGAGCTGAAA GCTCTACTCT ATGAGCCTCA GGAAGGTGAA 
TGGGGGGCTC ATACACGAGA TGAAGAATGT GAGCGAGTCA TTCATGGCAT CGATCAGCTT 
CTGACACTGG ATGTAGCCAA GGCGTTCGCA TTACCGGTAA ATTTGCAGGA CTACCCTCTG 
TACTGCACCG TAGTGGCTTA CCCCACTGAC CTCAGCTCCA TCCGCACACG TCTGGAGAAT 
CGCTTTTACA GGCGGATCTC TGCATTAATG TGGGAGGTGC GTTACATCGA ACACAATGCC 
CGCACTTTCA ATGAACCTCA AAGCCCCATT GTAGCAGCTG CCAAGGTGGT GACTGATGTT 
CTCCTTCATT ATATCGGGGA CCAAAGTTGC ATCGACGTCT TGGATCTGTA TCGTAAACTG 
AAGTCTGAAG TCAGCAGTGA AGCTGAGGTT GACTTGGACG TAGATTCAGA CACTCCAGGA 
ACATCTACAG GACACCGGGG AGGCACTGCA AATTCTAAGA AGCGTGGTGT GGTTCTGGAT 
CCGAAAGCCT GGCGAGGTCA ATGTCGAGAG CTGTTGCGTC GAATGACAGC TTCCACGAAT 
TCCGAACCTT TCAGGCAGCC TGTGGATCTC TTTGAATATC CGGACTATCG AGACATCATT 
GACACTCCCA TGGATCTGGG TACAGTGTCA GAGACCCTGG CAGTAGGAAA TTATGAAAAT 
CCCATGGAGT TTTCCAAAGA CATCCGCCTC ATTTTCAGCA ACTCTAAAGC ATACACTCCT 
AACAAGAAGT CACAGATCTA TACCATGACC CTCAGCTTGT CGGCCTTCTT TGAAAAACAC 
ATCATCTCCA TCATTTCTGA CTACAAGTCT GCCATTCAGA ACGAACGGCG GGCTCGTCAA 
CATCTCACTT ACAGGAACAG ATTGCACAAC GGAGGCAGCT CGCCACCTAA TAGTCCATCC 
CACAGCTCAA AAGGAAAGCA CAAGTCAGGG AAGGCATCAA AGCCAAAAAA ATCCCAAACC 
TCAACAAAGA ATCGTTCTCA GAGATCACAT AAAGCTCAGC AAAGCGGTAA TGTAGCAGAG 
GAAGAGGACG TCCAGCCTTC TTCCCCAACT TCAGGGACGA GTGTCAGGAG CAGAAATCAA 
GGACCACATC GGGTGACCCG CAGCCAAGCA ACTCCCGTGA AGAAATCAGG TCACCAGAAT 
AACTTGCACC TAAGTAACGG CTCCAGACAA CGGCACTTGC GAGAAACGTC GCAGTCCGAC 
GATGAAGAGG AGGCGTCAGG ATCCAACAGC TCCTCGTTGG AGTCGTCCTC CACCTCGTCG 
TCATCATCAT CATCATCATC ATCCTCCTCC TCCTCCTCAT CGTCGGACAG CGACAACAGC 
TCTGACTCTG AGGTGGAAAA GTACGTGGAG GGAGGAGGAG ATCACGACTA CAGCAAAGCC 
CCCTGCCTGT CAGTACGCCT CTCAGCTAAG GGTAAGGTGG CAGCCTCAGA AAAGAGGAGA 
CACAAAGGAG GAGAGGACAC AGAGGCACAG GGACCTAAAC GAGCACGACT GCAGCTTGAG 
GTAGAGGAGC AGCAGGATGA GGAGGAGGAG GAGGAAGAGG CAGAGGAGGA TGAGGAAGAA 
GAAGAAGAGG AGGCAGCAGC TCCAGCAGCA GCTAGCAGCC AGAGAGGCAG CCAGTGTAAG 
TCTCGGCGGG TCAACACACG AAACCAGGGC CGCAGGACAG TTCGATACGG AGACGACTCG 
GAGGATGATG GCCATGGGTC GAAAGACGAC CCTCTAAACC TGGGCATGTC CCGCTCAGGA 
CGGGTACGCC GTATGACTGA GAAAGCCCGT GTCAGCCACC TTATGGGCTG GAGTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012780793.1
CDS	277..5496
Translation	MAREQCSQIEAELYYLIARFLQSGPCKKAAKILIEELEEYELVPQRWSWDGKKYKRNFQDWVVLNQHIPADYLLRICQRIGPLIEKEIPPCVPGVQTLLGLGRQSLLRTSKSCSHKVCSGSAVAALHRGRPPEPPVSNGSSPNVVSIMGARQATGTGRFGQVLPSSSYQHMKIHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRGHSAEICDMAVNHENSLIASASCDKVIRVWCLRTCAPITVLQAHAASITSIQFCPAARGTTRYLASTGADGMVCFWKWHSLTMKFNDHPVKFMERSRPGVQVSTSSFSSGGMFMATGGTDHMIRVYYLGAESPMKVSEMDAHTDKVVVVQFSNNSDSLRFVSGSRDGTVKIWHYQQQEWKSTTLDMAARLPGSTAATGDDKAKLVVTMVAWDRTDSTIITAVSNYLLKVWSTSTGQLLHILSGHDDEVFVLEAHPFDSRIMLSAGHDGNIYIWDLTKGVKIRNFFNMIEGQGHGAIFDCKFSADGQHFACTDSHGHLLIFGFGCSRPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPSHYQRLVPGRENCKEEQLIPQMGYMANNDGELVEQVLGQQTAENGESPLDNLIRQLQNEQDERQNAEREAEGEEAGEGAEAVPSSPVVAPRRSGQVDGVWQMQHNALPSQVATERDLLAWSRRVVVNEVLQGIFRVSEECRQAKGDIECSLYYAERKKKPVTCAPKSDLLSSRLRSLRPTKKTHKRHAYQTRSAQIRRPGTRSRNPSNRRTSNSQMDSESDGEQAEEQVEQTDASSDGDARWQSESSSSDSSSEYSDWTADAGINLQPPKRPTRRPVQPPDYSSSEEEGDNEAKEARTREKEKRKKKSKETKQKPTSSLGGLDAEEWLPPSWIMETVPRCSPFVPQMGDELIYFKQGHQAYVRAVRRAKAYSINPQKQPWNRLNLRDQESVKVVGIKYEVGPPTLCCLKVAFLDPVSGKMTNESFSLKYHDMPDVIDFLVLQQFYNEAKEHNWQPGMRFRSIIDDAWWFGSVEDQEPLQPEYPDSLFQCYAVKWDNGEREKMSPWDMEPIPEEAALPEQVGDGVEVAEEELKALLYEPQEGEWGAHTRDEECERVIHGIDQLLTLDVAKAFALPVNLQDYPLYCTVVAYPTDLSSIRTRLENRFYRRISALMWEVRYIEHNARTFNEPQSPIVAAAKVVTDVLLHYIGDQSCIDVLDLYRKLKSEVSSEAEVDLDVDSDTPGTSTGHRGGTANSKKRGVVLDPKAWRGQCRELLRRMTASTNSEPFRQPVDLFEYPDYRDIIDTPMDLGTVSETLAVGNYENPMEFSKDIRLIFSNSKAYTPNKKSQIYTMTLSLSAFFEKHIISIISDYKSAIQNERRARQHLTYRNRLHNGGSSPPNSPSHSSKGKHKSGKASKPKKSQTSTKNRSQRSHKAQQSGNVAEEEDVQPSSPTSGTSVRSRNQGPHRVTRSQATPVKKSGHQNNLHLSNGSRQRHLRETSQSDDEEEASGSNSSSLESSSTSSSSSSSSSSSSSSSSSDSDNSSDSEVEKYVEGGGDHDYSKAPCLSVRLSAKGKVAASEKRRHKGGEDTEAQGPKRARLQLEVEEQQDEEEEEEEAEEDEEEEEEEAAAPAAASSQRGSQCKSRRVNTRNQGRRTVRYGDDSEDDGHGSKDDPLNLGMSRSGRVRRMTEKARVSHLMGWSH

Target ORF information:

RefSeq Version	XM_012925339.2
Organism	Maylandia zebra(Zebra mbuna)
Definition	Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012925339.2

ORF Insert Sequence:

ATGGCAAGAG AACAGTGTTC ACAAATCGAA GCTGAACTCT ATTACCTCAT TGCCAGATTT 
CTGCAGTCTG GACCATGTAA AAAAGCCGCA AAGATCCTGA TCGAGGAGCT GGAGGAGTAT 
GAGTTGGTCC CGCAACGATG GAGTTGGGAC GGGAAGAAAT ACAAACGGAA CTTCCAAGAT 
TGGGTGGTGT TGAACCAGCA CATTCCTGCA GACTATTTGC TCAGGATTTG CCAGCGGATA 
GGCCCACTTA TAGAAAAGGA GATCCCACCT TGTGTTCCTG GAGTCCAGAC ACTTCTGGGG 
CTGGGAAGAC AGTCCTTGCT TCGCACCAGC AAAAGTTGTA GCCACAAGGT ATGTAGTGGC 
TCAGCTGTCG CTGCACTCCA CCGAGGACGT CCACCAGAAC CTCCAGTGAG CAATGGAAGT 
TCTCCTAATG TTGTGTCCAT CATGGGTGCT CGCCAGGCCA CAGGCACAGG TCGTTTTGGC 
CAGGTTCTGC CGTCTTCCTC CTACCAACAC ATGAAGATAC ACAAACGTAT CCTTGGCCAC 
CTTTCTTCAG TCTACTGTGT GGCCTTTGAC CGTACCGGTC GTCGAATATT TACGGGTTCT 
GATGATTGCT TGGTAAAAAT CTGGGCCACA GATGATGGCC GGCTCCTGGC AACACTCCGC 
GGTCACTCAG CAGAGATCTG TGACATGGCT GTCAACCACG AGAACAGCCT CATTGCTTCT 
GCCAGCTGTG ACAAAGTCAT CAGAGTGTGG TGCTTACGCA CCTGTGCGCC CATCACTGTG 
CTGCAGGCTC ATGCTGCGTC TATCACATCC ATACAGTTTT GTCCAGCAGC TAGAGGAACA 
ACTCGGTACC TGGCATCAAC TGGTGCAGAC GGCATGGTGT GTTTCTGGAA GTGGCATTCG 
CTCACCATGA AATTTAATGA TCATCCAGTC AAGTTTATGG AGCGTTCACG GCCCGGGGTG 
CAAGTCTCCA CTTCATCTTT CAGCAGTGGT GGCATGTTCA TGGCTACTGG AGGCACTGAT 
CATATGATCA GGGTCTACTA TCTTGGTGCT GAGTCTCCTA TGAAGGTTTC TGAGATGGAT 
GCTCACACGG ATAAAGTCGT GGTCGTCCAG TTCAGCAATA ACAGTGACAG CCTAAGGTTC 
GTGAGCGGCA GTCGCGACGG TACGGTGAAG ATTTGGCATT ACCAGCAGCA GGAGTGGAAG 
AGCACCACTT TAGACATGGC TGCAAGGCTT CCAGGGAGCA CTGCCGCCAC AGGGGACGAC 
AAAGCTAAGT TGGTGGTGAC TATGGTTGCC TGGGACCGCA CTGACAGCAC CATAATCACA 
GCGGTGTCAA ACTACCTGCT CAAAGTGTGG AGCACATCCA CAGGACAGCT GCTGCACATT 
TTGTCTGGTC ATGACGACGA GGTGTTTGTG CTGGAGGCTC ATCCATTTGA CTCCCGCATC 
ATGTTATCTG CCGGCCACGA TGGCAACATT TACATCTGGG ACCTAACCAA AGGAGTCAAG 
ATCCGCAATT TCTTCAACAT GATTGAGGGC CAAGGCCACG GTGCTATTTT TGACTGCAAG 
TTCTCAGCAG ATGGGCAGCA TTTTGCCTGC ACTGACTCAC ACGGCCATTT GCTCATCTTT 
GGTTTTGGCT GCAGCAGACC ATATGAAAAG ATTCCCGACC AGATGTTCTT CCACACGGAC 
TATCGCCCTC TTATCCGTGA CTCTAATAAC TACGTCCTGG ATGAGCAGAC GCAACAGGCG 
CCACACCTCA TGCCTCCACC CTTCCTTGTG GATGTCGACG GGAATCCTCA TCCTTCTCAC 
TACCAGCGCC TGGTGCCTGG ACGGGAGAAC TGCAAGGAAG AGCAGCTAAT ACCTCAGATG 
GGATACATGG CTAACAATGA TGGAGAGTTG GTTGAGCAGG TTCTTGGCCA GCAAACGGCA 
GAAAATGGAG AAAGCCCATT GGACAATCTC ATCAGACAGC TGCAGAATGA ACAAGATGAG 
CGTCAGAATG CAGAGCGGGA GGCAGAAGGG GAAGAAGCTG GTGAAGGCGC AGAGGCGGTG 
CCCTCCTCTC CAGTTGTTGC ACCTCGCAGA AGTGGGCAGG TGGATGGGGT GTGGCAAATG 
CAGCATAACG CCCTTCCCAG TCAGGTGGCC ACCGAGAGGG ACCTGCTGGC CTGGAGCCGC 
AGAGTGGTGG TCAATGAAGT GCTGCAGGGG ATATTCAGAG TTTCAGAGGA GTGCAGACAA 
GCCAAAGGTG ACATTGAATG TTCTCTATAC TACGCTGAGA GGAAGAAGAA ACCAGTAACC 
TGTGCACCTA AGAGCGATCT CTTGTCTTCA CGCCTGCGCT CCCTGCGGCC CACGAAGAAA 
ACTCATAAGC GCCATGCCTA TCAAACCCGG TCAGCTCAAA TCCGGCGTCC TGGAACCAGG 
AGTAGAAATC CCAGCAACCG ACGTACCTCT AACAGCCAGA TGGACTCGGA GAGTGATGGA 
GAACAGGCAG AAGAACAGGT GGAGCAAACC GATGCATCTT CAGATGGTGA TGCTCGGTGG 
CAGAGTGAAA GCAGCTCCAG CGATTCCTCC AGTGAATATT CTGATTGGAC AGCGGATGCT 
GGGATCAATT TGCAGCCGCC AAAGCGACCT ACCAGGAGAC CTGTACAGCC TCCAGATTAC 
AGCAGCTCTG AGGAAGAAGG AGATAACGAA GCCAAGGAGG CAAGGACGAG GGAAAAAGAG 
AAGAGGAAGA AGAAGTCCAA GGAGACCAAA CAAAAACCCA CCTCCTCTCT GGGTGGACTT 
GATGCGGAAG AGTGGCTGCC GCCATCTTGG ATTATGGAGA CTGTCCCCCG CTGCTCTCCT 
TTTGTTCCAC AAATGGGAGA TGAACTCATC TACTTCAAAC AGGGCCATCA GGCTTACGTC 
CGGGCTGTTC GCAGAGCTAA GGCTTACAGC ATCAACCCTC AGAAGCAGCC GTGGAACAGA 
CTCAACCTCA GGGATCAGGA ATCTGTGAAA GTGGTTGGAA TTAAGTATGA AGTGGGACCT 
CCTACCCTCT GCTGCTTAAA AGTGGCGTTC TTGGACCCAG TCTCAGGGAA AATGACCAAT 
GAGTCTTTCT CCTTAAAGTA TCATGACATG CCTGATGTCA TTGATTTTCT GGTACTGCAG 
CAGTTTTACA ACGAGGCTAA AGAGCACAAC TGGCAACCTG GCATGAGGTT CCGCAGCATC 
ATTGACGACG CCTGGTGGTT CGGCTCTGTG GAGGATCAAG AGCCCCTGCA GCCAGAGTAT 
CCCGACAGCC TGTTTCAGTG CTACGCAGTA AAATGGGATA ACGGTGAGCG GGAGAAAATG 
AGTCCCTGGG ATATGGAGCC TATTCCTGAG GAAGCTGCTT TGCCAGAGCA GGTTGGTGAT 
GGTGTAGAGG TGGCAGAAGA GGAGCTGAAA GCTCTACTCT ATGAGCCTCA GGAAGGTGAA 
TGGGGGGCTC ATACACGAGA TGAAGAATGT GAGCGAGTCA TTCATGGCAT CGATCAGCTT 
CTGACACTGG ATGTAGCCAA GGCGTTCGCA TTACCGGTAA ATTTGCAGGA CTACCCTCTG 
TACTGCACCG TAGTGGCTTA CCCCACTGAC CTCAGCTCCA TCCGCACACG TCTGGAGAAT 
CGCTTTTACA GGCGGATCTC TGCATTAATG TGGGAGGTGC GTTACATCGA ACACAATGCC 
CGCACTTTCA ATGAACCTCA AAGCCCCATT GTAGCAGCTG CCAAGGTGGT GACTGATGTT 
CTCCTTCATT ATATCGGGGA CCAAAGTTGC ATCGACGTCT TGGATCTGTA TCGTAAACTG 
AAGTCTGAAG TCAGCAGTGA AGCTGAGGTT GACTTGGACG TAGATTCAGA CACTCCAGGA 
ACATCTACAG GACACCGGGG AGGCACTGCA AATTCTAAGA AGCGTGGTGT GGTTCTGGAT 
CCGAAAGCCT GGCGAGGTCA ATGTCGAGAG CTGTTGCGTC GAATGACAGC TTCCACGAAT 
TCCGAACCTT TCAGGCAGCC TGTGGATCTC TTTGAATATC CGGACTATCG AGACATCATT 
GACACTCCCA TGGATCTGGG TACAGTGTCA GAGACCCTGG CAGTAGGAAA TTATGAAAAT 
CCCATGGAGT TTTCCAAAGA CATCCGCCTC ATTTTCAGCA ACTCTAAAGC ATACACTCCT 
AACAAGAAGT CACAGATCTA TACCATGACC CTCAGCTTGT CGGCCTTCTT TGAAAAACAC 
ATCATCTCCA TCATTTCTGA CTACAAGTCT GCCATTCAGA ACGAACGGCG GGCTCGTCAA 
CATCTCACTT ACAGGAACAG ATTGCACAAC GGAGGCAGCT CGCCACCTAA TAGTCCATCC 
CACAGCTCAA AAGGAAAGCA CAAGTCAGGG AAGGCATCAA AGCCAAAAAA ATCCCAAACC 
TCAACAAAGA ATCGTTCTCA GAGATCACAT AAAGCTCAGC AAAGCGGTAA TGTAGCAGAG 
GAAGAGGACG TCCAGCCTTC TTCCCCAACT TCAGGGACGA GTGTCAGGAG CAGAAATCAA 
GGACCACATC GGGTGACCCG CAGCCAAGCA ACTCCCGTGA AGAAATCAGG TCACCAGAAT 
AACTTGCACC TAAGTAACGG CTCCAGACAA CGGCACTTGC GAGAAACGTC GCAGTCCGAC 
GATGAAGAGG AGGCGTCAGG ATCCAACAGC TCCTCGTTGG AGTCGTCCTC CACCTCGTCG 
TCATCATCAT CATCATCATC ATCCTCCTCC TCCTCCTCAT CGTCGGACAG CGACAACAGC 
TCTGACTCTG AGGTGGAAAA GTACGTGGAG GGAGGAGGAG ATCACGACTA CAGCAAAGCC 
CCCTGCCTGT CAGTACGCCT CTCAGCTAAG GGTAAGGTGG CAGCCTCAGA AAAGAGGAGA 
CACAAAGGAG GAGAGGACAC AGAGGCACAG GGACCTAAAC GAGCACGACT GCAGCTTGAG 
GTAGAGGAGC AGCAGGATGA GGAGGAGGAG GAGGAAGAGG CAGAGGAGGA TGAGGAAGAA 
GAAGAAGAGG AGGCAGCAGC TCCAGCAGCA GCTAGCAGCC AGAGAGGCAG CCAGTGTAAG 
TCTCGGCGGG TCAACACACG AAACCAGGGC CGCAGGACAG TTCGATACGG AGACGACTCG 
GAGGATGATG GCCATGGGTC GAAAGACGAC CCTCTAAACC TGGGCATGTC CCGCTCAGGA 
CGGGTACGCC GTATGACTGA GAAAGCCCGT GTCAGCCACC TTATGGGCTG GAGTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brwd3 ( XM_023155317.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023011085.1 Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.

Price & Availability↑

CloneID	OMa311742
Clone ID Related Accession (Same CDS sequence)	XM_023155317.1
Accession Version	XM_023155317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	bromodomain and WD repeat-containing protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447412.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAG AACAGTGTTC ACAAATCGAA GCTGAACTCT ATTACCTCAT TGCCAGATTT 
CTGCAGTCTG GACCATGTAA AAAAGCCGCA AAGATCCTGA TCGAGGAGCT GGAGGAGTAT 
GAGTTGGTCC CGCAACGATG GAGTTGGGAC GGGAAGAAAT ACAAACGGAA CTTCCAAGAT 
TGGGTGGTGT TGAACCAGCA CATTCCTGCA GACTATTTGC TCAGGATTTG CCAGCGGATA 
GGCCCACTTA TAGAAAAGGA GATCCCACCT TGTGTTCCTG GAGTCCAGAC ACTTCTGGGG 
CTGGGAAGAC AGTCCTTGCT TCGCACCAGC AAAAGTTGTA GCCACAAGGT ATGTAGTGGC 
TCAGCTGTCG CTGCACTCCA CCGAGGACGT CCACCAGAAC CTCCAGTGAG CAATGGAAGT 
TCTCCTAATG TTGTGTCCAT CATGGGTGCT CGCCAGGCCA CAGGCACAGG TCGTTTTGGC 
CAGGTTCTGC CGTCTTCCTC CTACCAACAC ATGAAGATAC ACAAACGTAT CCTTGGCCAC 
CTTTCTTCAG TCTACTGTGT GGCCTTTGAC CGTACCGGTC GTCGAATATT TACGGGTTCT 
GATGATTGCT TGGTAAAAAT CTGGGCCACA GATGATGGCC GGCTCCTGGC AACACTCCGC 
GGTCACTCAG CAGAGATCTG TGACATGGCT GTCAACCACG AGAACAGCCT CATTGCTTCT 
GCCAGCTGTG ACAAAGTCAT CAGAGTGTGG TGCTTACGCA CCTGTGCGCC CATCACTGTG 
CTGCAGGCTC ATGCTGCGTC TATCACATCC ATACAGTTTT GTCCAGCAGC TAGAGGAACA 
ACTCGGTACC TGGCATCAAC TGGTGCAGAC GGCATGGTGT GTTTCTGGAA GTGGCATTCG 
CTCACCATGA AATTTAATGA TCATCCAGTC AAGTTTATGG AGCGTTCACG GCCCGGGGTG 
CAAGTCTCCA CTTCATCTTT CAGCAGTGGT GGCATGTTCA TGGCTACTGG AGGCACTGAT 
CATATGATCA GGGTCTACTA TCTTGGTGCT GAGTCTCCTA TGAAGGTTTC TGAGATGGAT 
GCTCACACGG ATAAAGTCGT GGTCGTCCAG TTCAGCAATA ACAGTGACAG CCTAAGGTTC 
GTGAGCGGCA GTCGCGACGG TACGGTGAAG ATTTGGCATT ACCAGCAGCA GGAGTGGAAG 
AGCACCACTT TAGACATGGC TGCAAGGCTT CCAGGGAGCA CTGCCGCCAC AGGGGACGAC 
AAAGCTAAGT TGGTGGTGAC TATGGTTGCC TGGGACCGCA CTGACAGCAC CATAATCACA 
GCGGTGTCAA ACTACCTGCT CAAAGTGTGG AGCACATCCA CAGGACAGCT GCTGCACATT 
TTGTCTGGTC ATGACGACGA GGTGTTTGTG CTGGAGGCTC ATCCATTTGA CTCCCGCATC 
ATGTTATCTG CCGGCCACGA TGGCAACATT TACATCTGGG ACCTAACCAA AGGAGTCAAG 
ATCCGCAATT TCTTCAACAT GATTGAGGGC CAAGGCCACG GTGCTATTTT TGACTGCAAG 
TTCTCAGCAG ATGGGCAGCA TTTTGCCTGC ACTGACTCAC ACGGCCATTT GCTCATCTTT 
GGTTTTGGCT GCAGCAGACC ATATGAAAAG ATTCCCGACC AGATGTTCTT CCACACGGAC 
TATCGCCCTC TTATCCGTGA CTCTAATAAC TACGTCCTGG ATGAGCAGAC GCAACAGGCG 
CCACACCTCA TGCCTCCACC CTTCCTTGTG GATGTCGACG GGAATCCTCA TCCTTCTCAC 
TACCAGCGCC TGGTGCCTGG ACGGGAGAAC TGCAAGGAAG AGCAGCTAAT ACCTCAGATG 
GGATACATGG CTAACAATGA TGGAGAGTTG GTTGAGCAGG TTCTTGGCCA GCAAACGGCA 
GAAAATGGAG AAAGCCCATT GGACAATCTC ATCAGACAGC TGCAGAATGA ACAAGATGAG 
CGTCAGAATG CAGAGCGGGA GGCAGAAGGG GAAGAAGCTG GTGAAGGCGC AGAGGCGGTG 
CCCTCCTCTC CAGTTGTTGC ACCTCGCAGA AGTGGGCAGG TGGATGGGGT GTGGCAAATG 
CAGCATAACG CCCTTCCCAG TCAGGTGGCC ACCGAGAGGG ACCTGCTGGC CTGGAGCCGC 
AGAGTGGTGG TCAATGAAGT GCTGCAGGGG ATATTCAGAG TTTCAGAGGA GTGCAGACAA 
GCCAAAGGTG ACATTGAATG TTCTCTATAC TACGCTGAGA GGAAGAAGAA ACCAGTAACC 
TGTGCACCTA AGAGCGATCT CTTGTCTTCA CGCCTGCGCT CCCTGCGGCC CACGAAGAAA 
ACTCATAAGC GCCATGCCTA TCAAACCCGG TCAGCTCAAA TCCGGCGTCC TGGAACCAGG 
AGTAGAAATC CCAGCAACCG ACGTACCTCT AACAGCCAGA TGGACTCGGA GAGTGATGGA 
GAACAGGCAG AAGAACAGGT GGAGCAAACC GATGCATCTT CAGATGGTGA TGCTCGGTGG 
CAGAGTGAAA GCAGCTCCAG CGATTCCTCC AGTGAATATT CTGATTGGAC AGCGGATGCT 
GGGATCAATT TGCAGCCGCC AAAGCGACCT ACCAGGAGAC CTGTACAGCC TCCAGATTAC 
AGCAGCTCTG AGGAAGAAGG AGATAACGAA GCCAAGGAGG CAAGGACGAG GGAAAAAGAG 
AAGAGGAAGA AGAAGTCCAA GGAGACCAAA CAAAAACCCA CCTCCTCTCT GGGTGGACTT 
GATGCGGAAG AGTGGCTGCC GCCATCTTGG ATTATGGAGA CTGTCCCCCG CTGCTCTCCT 
TTTGTTCCAC AAATGGGAGA TGAACTCATC TACTTCAAAC AGGGCCATCA GGCTTACGTC 
CGGGCTGTTC GCAGAGCTAA GGCTTACAGC ATCAACCCTC AGAAGCAGCC GTGGAACAGA 
CTCAACCTCA GGGATCAGGA ATCTGTGAAA GTGGTTGGAA TTAAGTATGA AGTGGGACCT 
CCTACCCTCT GCTGCTTAAA AGTGGCGTTC TTGGACCCAG TCTCAGGGAA AATGACCAAT 
GAGTCTTTCT CCTTAAAGTA TCATGACATG CCTGATGTCA TTGATTTTCT GGTACTGCAG 
CAGTTTTACA ACGAGGCTAA AGAGCACAAC TGGCAACCTG GCATGAGGTT CCGCAGCATC 
ATTGACGACG CCTGGTGGTT CGGCTCTGTG GAGGATCAAG AGCCCCTGCA GCCAGAGTAT 
CCCGACAGCC TGTTTCAGTG CTACGCAGTA AAATGGGATA ACGGTGAGCG GGAGAAAATG 
AGTCCCTGGG ATATGGAGCC TATTCCTGAG GAAGCTGCTT TGCCAGAGCA GGTTGGTGAT 
GGTGTAGAGG TGGCAGAAGA GGAGCTGAAA GCTCTACTCT ATGAGCCTCA GGAAGGTGAA 
TGGGGGGCTC ATACACGAGA TGAAGAATGT GAGCGAGTCA TTCATGGCAT CGATCAGCTT 
CTGACACTGG ATGTAGCCAA GGCGTTCGCA TTACCGGTAA ATTTGCAGGA CTACCCTCTG 
TACTGCACCG TAGTGGCTTA CCCCACTGAC CTCAGCTCCA TCCGCACACG TCTGGAGAAT 
CGCTTTTACA GGCGGATCTC TGCATTAATG TGGGAGGTGC GTTACATCGA ACACAATGCC 
CGCACTTTCA ATGAACCTCA AAGCCCCATT GTAGCAGCTG CCAAGGTGGT GACTGATGTT 
CTCCTTCATT ATATCGGGGA CCAAAGTTGC ATCGACGTCT TGGATCTGTA TCGTAAACTG 
AAGTCTGAAG TCAGCAGTGA AGCTGAGGTT GACTTGGACG TAGATTCAGA CACTCCAGGA 
ACATCTACAG GACACCGGGG AGGCACTGCA AATTCTAAGA AGCGTGGTGT GGTTCTGGAT 
CCGAAAGCCT GGCGAGGTCA ATGTCGAGAG CTGTTGCGTC GAATGACAGC TTCCACGAAT 
TCCGAACCTT TCAGGCAGCC TGTGGATCTC TTTGAATATC CGGACTATCG AGACATCATT 
GACACTCCCA TGGATCTGGG TACAGTGTCA GAGACCCTGG CAGTAGGAAA TTATGAAAAT 
CCCATGGAGT TTTCCAAAGA CATCCGCCTC ATTTTCAGCA ACTCTAAAGC ATACACTCCT 
AACAAGAAGT CACAGATCTA TACCATGACC CTCAGCTTGT CGGCCTTCTT TGAAAAACAC 
ATCATCTCCA TCATTTCTGA CTACAAGTCT GCCATTCAGA ACGAACGGCG GGCTCGTCAA 
CATCTCACTT ACAGGAACAG ATTGCACAAC GGAGGCAGCT CGCCACCTAA TAGTCCATCC 
CACAGCTCAA AAGGAAAGCA CAAGTCAGGG AAGGCATCAA AGCCAAAAAA ATCCCAAACC 
TCAACAAAGA ATCGTTCTCA GAGATCACAT AAAGCTCAGC AAAGCGGTAA TGTAGCAGAG 
GAAGAGGACG TCCAGCCTTC TTCCCCAACT TCAGGGACGA GTGTCAGGAG CAGAAATCAA 
GGACCACATC GGGTGACCCG CAGCCAAGCA ACTCCCGTGA AGAAATCAGG TCACCAGAAT 
AACTTGCACC TAAGTAACGG CTCCAGACAA CGGCACTTGC GAGAAACGTC GCAGTCCGAC 
GATGAAGAGG AGGCGTCAGG ATCCAACAGC TCCTCGTTGG AGTCGTCCTC CACCTCGTCG 
TCATCATCAT CATCATCATC ATCCTCCTCC TCCTCCTCAT CGTCGGACAG CGACAACAGC 
TCTGACTCTG AGGTGGAAAA GTACGTGGAG GGAGGAGGAG ATCACGACTA CAGCAAAGCC 
CCCTGCCTGT CAGTACGCCT CTCAGCTAAG GGTAAGGTGG CAGCCTCAGA AAAGAGGAGA 
CACAAAGGAG GAGAGGACAC AGAGGCACAG GGACCTAAAC GAGCACGACT GCAGCTTGAG 
GTAGAGGAGC AGCAGGATGA GGAGGAGGAG GAGGAAGAGG AGGAAGAAGA AGAAGAAGAG 
GAGGAAGAAG AAGAAGAGGA GGAAGAAGAG GAAGAGGAGG AGCCAGAGGA CGAGGATGAT 
AACAACTCTG AGGTGCAGAC ACGAGCAGCA GCAGCTCCAG CAGCAGCTAG CAGCCAGAGA 
GGCAGCCAGT GTAAGTCTCG GCGGGTCAAC ACACGAAACC AGGGCCGCAG GACAGTTCGA 
TACGGAGACG ACTCGGAGGA TGATGGCCAT GGGTCGAAAG ACGACCCTCT AAACCTGGGC 
ATGTCCCGCT CAGGACGGGT ACGCCGTATG ACTGAGAAAG CCCGTGTCAG CCACCTTATG 
GGCTGGAGTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023011085.1
CDS	277..5571
Translation	MAREQCSQIEAELYYLIARFLQSGPCKKAAKILIEELEEYELVPQRWSWDGKKYKRNFQDWVVLNQHIPADYLLRICQRIGPLIEKEIPPCVPGVQTLLGLGRQSLLRTSKSCSHKVCSGSAVAALHRGRPPEPPVSNGSSPNVVSIMGARQATGTGRFGQVLPSSSYQHMKIHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRGHSAEICDMAVNHENSLIASASCDKVIRVWCLRTCAPITVLQAHAASITSIQFCPAARGTTRYLASTGADGMVCFWKWHSLTMKFNDHPVKFMERSRPGVQVSTSSFSSGGMFMATGGTDHMIRVYYLGAESPMKVSEMDAHTDKVVVVQFSNNSDSLRFVSGSRDGTVKIWHYQQQEWKSTTLDMAARLPGSTAATGDDKAKLVVTMVAWDRTDSTIITAVSNYLLKVWSTSTGQLLHILSGHDDEVFVLEAHPFDSRIMLSAGHDGNIYIWDLTKGVKIRNFFNMIEGQGHGAIFDCKFSADGQHFACTDSHGHLLIFGFGCSRPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPSHYQRLVPGRENCKEEQLIPQMGYMANNDGELVEQVLGQQTAENGESPLDNLIRQLQNEQDERQNAEREAEGEEAGEGAEAVPSSPVVAPRRSGQVDGVWQMQHNALPSQVATERDLLAWSRRVVVNEVLQGIFRVSEECRQAKGDIECSLYYAERKKKPVTCAPKSDLLSSRLRSLRPTKKTHKRHAYQTRSAQIRRPGTRSRNPSNRRTSNSQMDSESDGEQAEEQVEQTDASSDGDARWQSESSSSDSSSEYSDWTADAGINLQPPKRPTRRPVQPPDYSSSEEEGDNEAKEARTREKEKRKKKSKETKQKPTSSLGGLDAEEWLPPSWIMETVPRCSPFVPQMGDELIYFKQGHQAYVRAVRRAKAYSINPQKQPWNRLNLRDQESVKVVGIKYEVGPPTLCCLKVAFLDPVSGKMTNESFSLKYHDMPDVIDFLVLQQFYNEAKEHNWQPGMRFRSIIDDAWWFGSVEDQEPLQPEYPDSLFQCYAVKWDNGEREKMSPWDMEPIPEEAALPEQVGDGVEVAEEELKALLYEPQEGEWGAHTRDEECERVIHGIDQLLTLDVAKAFALPVNLQDYPLYCTVVAYPTDLSSIRTRLENRFYRRISALMWEVRYIEHNARTFNEPQSPIVAAAKVVTDVLLHYIGDQSCIDVLDLYRKLKSEVSSEAEVDLDVDSDTPGTSTGHRGGTANSKKRGVVLDPKAWRGQCRELLRRMTASTNSEPFRQPVDLFEYPDYRDIIDTPMDLGTVSETLAVGNYENPMEFSKDIRLIFSNSKAYTPNKKSQIYTMTLSLSAFFEKHIISIISDYKSAIQNERRARQHLTYRNRLHNGGSSPPNSPSHSSKGKHKSGKASKPKKSQTSTKNRSQRSHKAQQSGNVAEEEDVQPSSPTSGTSVRSRNQGPHRVTRSQATPVKKSGHQNNLHLSNGSRQRHLRETSQSDDEEEASGSNSSSLESSSTSSSSSSSSSSSSSSSSSDSDNSSDSEVEKYVEGGGDHDYSKAPCLSVRLSAKGKVAASEKRRHKGGEDTEAQGPKRARLQLEVEEQQDEEEEEEEEEEEEEEEEEEEEEEEEEEEEPEDEDDNNSEVQTRAAAAPAAASSQRGSQCKSRRVNTRNQGRRTVRYGDDSEDDGHGSKDDPLNLGMSRSGRVRRMTEKARVSHLMGWSH

Target ORF information:

RefSeq Version	XM_023155317.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023155317.1

ORF Insert Sequence:

ATGGCAAGAG AACAGTGTTC ACAAATCGAA GCTGAACTCT ATTACCTCAT TGCCAGATTT 
CTGCAGTCTG GACCATGTAA AAAAGCCGCA AAGATCCTGA TCGAGGAGCT GGAGGAGTAT 
GAGTTGGTCC CGCAACGATG GAGTTGGGAC GGGAAGAAAT ACAAACGGAA CTTCCAAGAT 
TGGGTGGTGT TGAACCAGCA CATTCCTGCA GACTATTTGC TCAGGATTTG CCAGCGGATA 
GGCCCACTTA TAGAAAAGGA GATCCCACCT TGTGTTCCTG GAGTCCAGAC ACTTCTGGGG 
CTGGGAAGAC AGTCCTTGCT TCGCACCAGC AAAAGTTGTA GCCACAAGGT ATGTAGTGGC 
TCAGCTGTCG CTGCACTCCA CCGAGGACGT CCACCAGAAC CTCCAGTGAG CAATGGAAGT 
TCTCCTAATG TTGTGTCCAT CATGGGTGCT CGCCAGGCCA CAGGCACAGG TCGTTTTGGC 
CAGGTTCTGC CGTCTTCCTC CTACCAACAC ATGAAGATAC ACAAACGTAT CCTTGGCCAC 
CTTTCTTCAG TCTACTGTGT GGCCTTTGAC CGTACCGGTC GTCGAATATT TACGGGTTCT 
GATGATTGCT TGGTAAAAAT CTGGGCCACA GATGATGGCC GGCTCCTGGC AACACTCCGC 
GGTCACTCAG CAGAGATCTG TGACATGGCT GTCAACCACG AGAACAGCCT CATTGCTTCT 
GCCAGCTGTG ACAAAGTCAT CAGAGTGTGG TGCTTACGCA CCTGTGCGCC CATCACTGTG 
CTGCAGGCTC ATGCTGCGTC TATCACATCC ATACAGTTTT GTCCAGCAGC TAGAGGAACA 
ACTCGGTACC TGGCATCAAC TGGTGCAGAC GGCATGGTGT GTTTCTGGAA GTGGCATTCG 
CTCACCATGA AATTTAATGA TCATCCAGTC AAGTTTATGG AGCGTTCACG GCCCGGGGTG 
CAAGTCTCCA CTTCATCTTT CAGCAGTGGT GGCATGTTCA TGGCTACTGG AGGCACTGAT 
CATATGATCA GGGTCTACTA TCTTGGTGCT GAGTCTCCTA TGAAGGTTTC TGAGATGGAT 
GCTCACACGG ATAAAGTCGT GGTCGTCCAG TTCAGCAATA ACAGTGACAG CCTAAGGTTC 
GTGAGCGGCA GTCGCGACGG TACGGTGAAG ATTTGGCATT ACCAGCAGCA GGAGTGGAAG 
AGCACCACTT TAGACATGGC TGCAAGGCTT CCAGGGAGCA CTGCCGCCAC AGGGGACGAC 
AAAGCTAAGT TGGTGGTGAC TATGGTTGCC TGGGACCGCA CTGACAGCAC CATAATCACA 
GCGGTGTCAA ACTACCTGCT CAAAGTGTGG AGCACATCCA CAGGACAGCT GCTGCACATT 
TTGTCTGGTC ATGACGACGA GGTGTTTGTG CTGGAGGCTC ATCCATTTGA CTCCCGCATC 
ATGTTATCTG CCGGCCACGA TGGCAACATT TACATCTGGG ACCTAACCAA AGGAGTCAAG 
ATCCGCAATT TCTTCAACAT GATTGAGGGC CAAGGCCACG GTGCTATTTT TGACTGCAAG 
TTCTCAGCAG ATGGGCAGCA TTTTGCCTGC ACTGACTCAC ACGGCCATTT GCTCATCTTT 
GGTTTTGGCT GCAGCAGACC ATATGAAAAG ATTCCCGACC AGATGTTCTT CCACACGGAC 
TATCGCCCTC TTATCCGTGA CTCTAATAAC TACGTCCTGG ATGAGCAGAC GCAACAGGCG 
CCACACCTCA TGCCTCCACC CTTCCTTGTG GATGTCGACG GGAATCCTCA TCCTTCTCAC 
TACCAGCGCC TGGTGCCTGG ACGGGAGAAC TGCAAGGAAG AGCAGCTAAT ACCTCAGATG 
GGATACATGG CTAACAATGA TGGAGAGTTG GTTGAGCAGG TTCTTGGCCA GCAAACGGCA 
GAAAATGGAG AAAGCCCATT GGACAATCTC ATCAGACAGC TGCAGAATGA ACAAGATGAG 
CGTCAGAATG CAGAGCGGGA GGCAGAAGGG GAAGAAGCTG GTGAAGGCGC AGAGGCGGTG 
CCCTCCTCTC CAGTTGTTGC ACCTCGCAGA AGTGGGCAGG TGGATGGGGT GTGGCAAATG 
CAGCATAACG CCCTTCCCAG TCAGGTGGCC ACCGAGAGGG ACCTGCTGGC CTGGAGCCGC 
AGAGTGGTGG TCAATGAAGT GCTGCAGGGG ATATTCAGAG TTTCAGAGGA GTGCAGACAA 
GCCAAAGGTG ACATTGAATG TTCTCTATAC TACGCTGAGA GGAAGAAGAA ACCAGTAACC 
TGTGCACCTA AGAGCGATCT CTTGTCTTCA CGCCTGCGCT CCCTGCGGCC CACGAAGAAA 
ACTCATAAGC GCCATGCCTA TCAAACCCGG TCAGCTCAAA TCCGGCGTCC TGGAACCAGG 
AGTAGAAATC CCAGCAACCG ACGTACCTCT AACAGCCAGA TGGACTCGGA GAGTGATGGA 
GAACAGGCAG AAGAACAGGT GGAGCAAACC GATGCATCTT CAGATGGTGA TGCTCGGTGG 
CAGAGTGAAA GCAGCTCCAG CGATTCCTCC AGTGAATATT CTGATTGGAC AGCGGATGCT 
GGGATCAATT TGCAGCCGCC AAAGCGACCT ACCAGGAGAC CTGTACAGCC TCCAGATTAC 
AGCAGCTCTG AGGAAGAAGG AGATAACGAA GCCAAGGAGG CAAGGACGAG GGAAAAAGAG 
AAGAGGAAGA AGAAGTCCAA GGAGACCAAA CAAAAACCCA CCTCCTCTCT GGGTGGACTT 
GATGCGGAAG AGTGGCTGCC GCCATCTTGG ATTATGGAGA CTGTCCCCCG CTGCTCTCCT 
TTTGTTCCAC AAATGGGAGA TGAACTCATC TACTTCAAAC AGGGCCATCA GGCTTACGTC 
CGGGCTGTTC GCAGAGCTAA GGCTTACAGC ATCAACCCTC AGAAGCAGCC GTGGAACAGA 
CTCAACCTCA GGGATCAGGA ATCTGTGAAA GTGGTTGGAA TTAAGTATGA AGTGGGACCT 
CCTACCCTCT GCTGCTTAAA AGTGGCGTTC TTGGACCCAG TCTCAGGGAA AATGACCAAT 
GAGTCTTTCT CCTTAAAGTA TCATGACATG CCTGATGTCA TTGATTTTCT GGTACTGCAG 
CAGTTTTACA ACGAGGCTAA AGAGCACAAC TGGCAACCTG GCATGAGGTT CCGCAGCATC 
ATTGACGACG CCTGGTGGTT CGGCTCTGTG GAGGATCAAG AGCCCCTGCA GCCAGAGTAT 
CCCGACAGCC TGTTTCAGTG CTACGCAGTA AAATGGGATA ACGGTGAGCG GGAGAAAATG 
AGTCCCTGGG ATATGGAGCC TATTCCTGAG GAAGCTGCTT TGCCAGAGCA GGTTGGTGAT 
GGTGTAGAGG TGGCAGAAGA GGAGCTGAAA GCTCTACTCT ATGAGCCTCA GGAAGGTGAA 
TGGGGGGCTC ATACACGAGA TGAAGAATGT GAGCGAGTCA TTCATGGCAT CGATCAGCTT 
CTGACACTGG ATGTAGCCAA GGCGTTCGCA TTACCGGTAA ATTTGCAGGA CTACCCTCTG 
TACTGCACCG TAGTGGCTTA CCCCACTGAC CTCAGCTCCA TCCGCACACG TCTGGAGAAT 
CGCTTTTACA GGCGGATCTC TGCATTAATG TGGGAGGTGC GTTACATCGA ACACAATGCC 
CGCACTTTCA ATGAACCTCA AAGCCCCATT GTAGCAGCTG CCAAGGTGGT GACTGATGTT 
CTCCTTCATT ATATCGGGGA CCAAAGTTGC ATCGACGTCT TGGATCTGTA TCGTAAACTG 
AAGTCTGAAG TCAGCAGTGA AGCTGAGGTT GACTTGGACG TAGATTCAGA CACTCCAGGA 
ACATCTACAG GACACCGGGG AGGCACTGCA AATTCTAAGA AGCGTGGTGT GGTTCTGGAT 
CCGAAAGCCT GGCGAGGTCA ATGTCGAGAG CTGTTGCGTC GAATGACAGC TTCCACGAAT 
TCCGAACCTT TCAGGCAGCC TGTGGATCTC TTTGAATATC CGGACTATCG AGACATCATT 
GACACTCCCA TGGATCTGGG TACAGTGTCA GAGACCCTGG CAGTAGGAAA TTATGAAAAT 
CCCATGGAGT TTTCCAAAGA CATCCGCCTC ATTTTCAGCA ACTCTAAAGC ATACACTCCT 
AACAAGAAGT CACAGATCTA TACCATGACC CTCAGCTTGT CGGCCTTCTT TGAAAAACAC 
ATCATCTCCA TCATTTCTGA CTACAAGTCT GCCATTCAGA ACGAACGGCG GGCTCGTCAA 
CATCTCACTT ACAGGAACAG ATTGCACAAC GGAGGCAGCT CGCCACCTAA TAGTCCATCC 
CACAGCTCAA AAGGAAAGCA CAAGTCAGGG AAGGCATCAA AGCCAAAAAA ATCCCAAACC 
TCAACAAAGA ATCGTTCTCA GAGATCACAT AAAGCTCAGC AAAGCGGTAA TGTAGCAGAG 
GAAGAGGACG TCCAGCCTTC TTCCCCAACT TCAGGGACGA GTGTCAGGAG CAGAAATCAA 
GGACCACATC GGGTGACCCG CAGCCAAGCA ACTCCCGTGA AGAAATCAGG TCACCAGAAT 
AACTTGCACC TAAGTAACGG CTCCAGACAA CGGCACTTGC GAGAAACGTC GCAGTCCGAC 
GATGAAGAGG AGGCGTCAGG ATCCAACAGC TCCTCGTTGG AGTCGTCCTC CACCTCGTCG 
TCATCATCAT CATCATCATC ATCCTCCTCC TCCTCCTCAT CGTCGGACAG CGACAACAGC 
TCTGACTCTG AGGTGGAAAA GTACGTGGAG GGAGGAGGAG ATCACGACTA CAGCAAAGCC 
CCCTGCCTGT CAGTACGCCT CTCAGCTAAG GGTAAGGTGG CAGCCTCAGA AAAGAGGAGA 
CACAAAGGAG GAGAGGACAC AGAGGCACAG GGACCTAAAC GAGCACGACT GCAGCTTGAG 
GTAGAGGAGC AGCAGGATGA GGAGGAGGAG GAGGAAGAGG AGGAAGAAGA AGAAGAAGAG 
GAGGAAGAAG AAGAAGAGGA GGAAGAAGAG GAAGAGGAGG AGCCAGAGGA CGAGGATGAT 
AACAACTCTG AGGTGCAGAC ACGAGCAGCA GCAGCTCCAG CAGCAGCTAG CAGCCAGAGA 
GGCAGCCAGT GTAAGTCTCG GCGGGTCAAC ACACGAAACC AGGGCCGCAG GACAGTTCGA 
TACGGAGACG ACTCGGAGGA TGATGGCCAT GGGTCGAAAG ACGACCCTCT AAACCTGGGC 
ATGTCCCGCT CAGGACGGGT ACGCCGTATG ACTGAGAAAG CCCGTGTCAG CCACCTTATG 
GGCTGGAGTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brwd3 ( XM_023155317.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023011085.1 Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.

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CloneID	OMa359830
Clone ID Related Accession (Same CDS sequence)	XM_023155317.2
Accession Version	XM_023155317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	bromodomain and WD repeat-containing protein 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036789.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023155317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGAG AACAGTGTTC ACAAATCGAA GCTGAACTCT ATTACCTCAT TGCCAGATTT 
CTGCAGTCTG GACCATGTAA AAAAGCCGCA AAGATCCTGA TCGAGGAGCT GGAGGAGTAT 
GAGTTGGTCC CGCAACGATG GAGTTGGGAC GGGAAGAAAT ACAAACGGAA CTTCCAAGAT 
TGGGTGGTGT TGAACCAGCA CATTCCTGCA GACTATTTGC TCAGGATTTG CCAGCGGATA 
GGCCCACTTA TAGAAAAGGA GATCCCACCT TGTGTTCCTG GAGTCCAGAC ACTTCTGGGG 
CTGGGAAGAC AGTCCTTGCT TCGCACCAGC AAAAGTTGTA GCCACAAGGT ATGTAGTGGC 
TCAGCTGTCG CTGCACTCCA CCGAGGACGT CCACCAGAAC CTCCAGTGAG CAATGGAAGT 
TCTCCTAATG TTGTGTCCAT CATGGGTGCT CGCCAGGCCA CAGGCACAGG TCGTTTTGGC 
CAGGTTCTGC CGTCTTCCTC CTACCAACAC ATGAAGATAC ACAAACGTAT CCTTGGCCAC 
CTTTCTTCAG TCTACTGTGT GGCCTTTGAC CGTACCGGTC GTCGAATATT TACGGGTTCT 
GATGATTGCT TGGTAAAAAT CTGGGCCACA GATGATGGCC GGCTCCTGGC AACACTCCGC 
GGTCACTCAG CAGAGATCTG TGACATGGCT GTCAACCACG AGAACAGCCT CATTGCTTCT 
GCCAGCTGTG ACAAAGTCAT CAGAGTGTGG TGCTTACGCA CCTGTGCGCC CATCACTGTG 
CTGCAGGCTC ATGCTGCGTC TATCACATCC ATACAGTTTT GTCCAGCAGC TAGAGGAACA 
ACTCGGTACC TGGCATCAAC TGGTGCAGAC GGCATGGTGT GTTTCTGGAA GTGGCATTCG 
CTCACCATGA AATTTAATGA TCATCCAGTC AAGTTTATGG AGCGTTCACG GCCCGGGGTG 
CAAGTCTCCA CTTCATCTTT CAGCAGTGGT GGCATGTTCA TGGCTACTGG AGGCACTGAT 
CATATGATCA GGGTCTACTA TCTTGGTGCT GAGTCTCCTA TGAAGGTTTC TGAGATGGAT 
GCTCACACGG ATAAAGTCGT GGTCGTCCAG TTCAGCAATA ACAGTGACAG CCTAAGGTTC 
GTGAGCGGCA GTCGCGACGG TACGGTGAAG ATTTGGCATT ACCAGCAGCA GGAGTGGAAG 
AGCACCACTT TAGACATGGC TGCAAGGCTT CCAGGGAGCA CTGCCGCCAC AGGGGACGAC 
AAAGCTAAGT TGGTGGTGAC TATGGTTGCC TGGGACCGCA CTGACAGCAC CATAATCACA 
GCGGTGTCAA ACTACCTGCT CAAAGTGTGG AGCACATCCA CAGGACAGCT GCTGCACATT 
TTGTCTGGTC ATGACGACGA GGTGTTTGTG CTGGAGGCTC ATCCATTTGA CTCCCGCATC 
ATGTTATCTG CCGGCCACGA TGGCAACATT TACATCTGGG ACCTAACCAA AGGAGTCAAG 
ATCCGCAATT TCTTCAACAT GATTGAGGGC CAAGGCCACG GTGCTATTTT TGACTGCAAG 
TTCTCAGCAG ATGGGCAGCA TTTTGCCTGC ACTGACTCAC ACGGCCATTT GCTCATCTTT 
GGTTTTGGCT GCAGCAGACC ATATGAAAAG ATTCCCGACC AGATGTTCTT CCACACGGAC 
TATCGCCCTC TTATCCGTGA CTCTAATAAC TATGTCCTGG ATGAGCAGAC GCAACAGGCG 
CCACACCTCA TGCCTCCACC CTTCCTTGTG GATGTCGACG GGAATCCTCA TCCTTCTCAC 
TACCAGCGCC TGGTGCCTGG ACGGGAGAAC TGCAAGGAAG AGCAGCTAAT ACCTCAGATG 
GGATACATGG CTAACAATGA TGGAGAGTTG GTTGAGCAGG TTCTTGGCCA GCAAACGGCA 
GAAAATGGAG AAAGCCCATT GGACAATCTC ATCAGACAGC TGCAGAATGA ACAAGATGAG 
CGTCAGAATG CAGAGCGGGA GGCAGAAGGG GAAGAAGCTG GTGAAGGCGC AGAGGCGGTG 
CCCTCCTCTC CAGTTGTTGC ACCTCGCAGA AGTGGGCAGG TGGATGGGGT GTGGCAAATG 
CAGCATAACG CCCTTCCCAG TCAGGTGGCC ACCGAGAGGG ACCTGCTGGC CTGGAGCCGC 
AGAGTGGTGG TCAATGAAGT GCTGCAGGGG ATATTCAGAG TTTCAGAGGA GTGCAGACAA 
GCCAAAGGTG ACATTGAATG TTCTCTATAC TACGCTGAGA GGAAGAAGAA ACCAGTAACC 
TGTGCACCTA AGAGCGATCT CTTGTCTTCA CGCCTGCGCT CCCTGCGGCC CACGAAGAAA 
ACTCATAAGC GCCATGCCTA TCAAACCCGG TCAGCTCAAA TCCGGCGTCC TGGAACCAGG 
AGTAGAAATC CCAGCAACCG ACGTACCTCT AACAGCCAGA TGGACTCGGA GAGTGATGGA 
GAACAGGCAG AAGAACAGGT GGAGCAAACC GATGCATCTT CAGATGGTGA TGCTCGGTGG 
CAGAGTGAAA GCAGCTCCAG CGATTCCTCC AGTGAATATT CTGATTGGAC AGCGGATGCT 
GGGATCAATT TGCAGCCGCC AAAGCGACCT ACCAGGAGAC CTGTACAGCC TCCAGATTAC 
AGCAGCTCTG AGGAAGAAGG AGATAACGAA GCCAAGGAGG CAAGGACGAG GGAAAAAGAG 
AAGAGGAAGA AGAAGTCCAA GGAGACCAAA CAAAAACCCA CCTCCTCTCT GGGTGGACTT 
GATGCGGAAG AGTGGCTGCC GCCATCTTGG ATTATGGAGA CTGTCCCCCG CTGCTCTCCT 
TTTGTTCCAC AAATGGGAGA TGAACTCATC TACTTCAAAC AGGGCCATCA GGCTTACGTC 
CGGGCTGTTC GCAGAGCTAA GGCTTACAGC ATCAACCCTC AGAAGCAGCC GTGGAACAGA 
CTCAACCTCA GGGATCAGGA ATCTGTGAAA GTGGTTGGAA TTAAGTATGA AGTGGGACCT 
CCTACCCTCT GCTGCTTAAA AGTGGCGTTC TTGGACCCAG TCTCAGGGAA AATGACCAAT 
GAGTCTTTCT CCTTAAAGTA TCATGACATG CCTGATGTCA TTGATTTTCT GGTACTGCAG 
CAGTTTTACA ACGAGGCTAA AGAGCACAAC TGGCAACCTG GCATGAGGTT CCGCAGCATC 
ATTGACGACG CCTGGTGGTT CGGCTCTGTG GAGGATCAAG AGCCCCTGCA GCCAGAGTAT 
CCCGACAGCC TGTTTCAGTG CTACGCAGTA AAATGGGATA ACGGTGAGCG GGAGAAAATG 
AGTCCCTGGG ATATGGAGCC TATTCCTGAG GAAGCTGCTT TGCCAGAGCA GGTTGGTGAT 
GGTGTAGAGG TGGCAGAAGA GGAGCTGAAA GCTCTACTCT ATGAGCCTCA GGAAGGTGAA 
TGGGGGGCTC ATACACGAGA TGAAGAATGT GAGCGAGTCA TTCATGGCAT CGATCAGCTT 
CTGACACTGG ATGTAGCCAA GGCGTTCGCA TTACCGGTAA ATTTGCAGGA CTACCCTCTG 
TACTGCACCG TAGTGGCTTA CCCCACTGAC CTCAGCTCCA TCCGCACACG TCTGGAGAAT 
CGCTTTTACA GGCGGATCTC TGCATTAATG TGGGAGGTGC GTTACATCGA ACACAATGCC 
CGCACTTTCA ATGAACCTCA AAGCCCCATT GTAGCAGCTG CCAAGGTGGT GACTGATGTT 
CTCCTTCATT ATATCGGGGA CCAAAGTTGC ATCGATGTCT TGGATCTGTA TCGTAAACTG 
AAGTCTGAAG TCAGCAGTGA AGCTGAGGTT GACTTGGACG TAGATTCAGA CACTCCAGGA 
ACATCTACAG GACACCGGGG AGGCACTGCA AATTCTAAGA AGCGTGGTGT GGTTCTGGAT 
CCGAAAGCCT GGCGAGGTCA ATGTCGAGAG CTGTTGCGTC GAATGACAGC TTCCACGAAT 
TCCGAACCTT TCAGGCAGCC TGTGGATCTC TTTGAATATC CGGACTATCG AGACATCATT 
GACACTCCCA TGGATCTGGG TACAGTGTCA GAGACCCTGG CAGTAGGAAA TTATGAAAAT 
CCCATGGAGT TTTCCAAAGA CATCCGCCTC ATTTTCAGCA ACTCTAAAGC ATACACTCCT 
AACAAGAAGT CACAGATCTA TACCATGACC CTCAGCTTGT CGGCCTTCTT TGAAAAACAC 
ATCATCTCCA TCATTTCTGA CTACAAGTCT GCCATTCAGA ACGAACGGCG GGCTCGTCAA 
CATCTCACTT ACAGGAACAG ATTGCACAAC GGAGGCAGCT CGCCACCTAA TAGTCCATCC 
CACAGCTCAA AAGGAAAGCA CAAGTCAGGG AAGGCATCAA AGCCAAAAAA ATCCCAAACC 
TCAACAAAGA ATCGTTCTCA GAGATCACAT AAAGCTCAGC AAAGCGGTAA TGTAGCAGAG 
GAAGAGGACG TCCAGCCTTC TTCCCCAACT TCAGGGACGA GTGTCAGGAG CAGAAATCAA 
GGACCACATC GGGTGACCCG CAGCCAAGCA ACTCCCGTGA AGAAATCAGG TCACCAGAAT 
AACTTGCACC TAAGTAACGG CTCCAGACAA CGGCACTTGC GAGAAACGTC GCAGTCCGAC 
GATGAAGAGG AGGCGTCAGG ATCCAACAGC TCCTCGTTGG AGTCGTCCTC CACCTCGTCG 
TCATCATCAT CATCATCATC ATCCTCCTCC TCCTCCTCAT CGTCGGACAG CGACAACAGC 
TCTGACTCTG AGGTGGAAAA GTACGTGGAG GGAGGAGGAG ATCACGACTA CAGCAAAGCC 
CCCTGCCTGT CAGTACGCCT CTCAGCTAAG GGTAAGGTGG CAGCCTCAGA AAAGAGGAGA 
CACAAAGGAG GAGAGGACAC AGAGGCACAG GGACCTAAAC GAGCACGACT GCAGCTTGAG 
GTAGAGGAGC AGCAGGATGA GGAGGAGGAG GAGGAAGAGG AGGAAGAAGA AGAAGAAGAG 
GAGGAAGAAG AAGAAGAGGA GGAAGAAGAG GAAGAGGAGG AGCCAGAGGA CGAGGATGAT 
AACAACTCTG AGGTGCAGAC ACGAGCAGCA GCAGCTCCAG CAGCAGCTAG CAGCCAGAGA 
GGCAGCCAGT GTAAGTCTCG GCGGGTCAAC ACACGAAACC AGGGCCGCAG GACAGTTCGA 
TACGGAGACG ACTCGGAGGA TGATGGCCAT GGGTCGAAAG ACGACCCTCT AAACCTGGGC 
ATGTCCCGCT CAGGACGGGT ACGCCGTATG ACTGAGAAAG CTCGTGTCAG CCACCTTATG 
GGCTGGAGTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023011085.1
CDS	277..5571
Translation	MAREQCSQIEAELYYLIARFLQSGPCKKAAKILIEELEEYELVPQRWSWDGKKYKRNFQDWVVLNQHIPADYLLRICQRIGPLIEKEIPPCVPGVQTLLGLGRQSLLRTSKSCSHKVCSGSAVAALHRGRPPEPPVSNGSSPNVVSIMGARQATGTGRFGQVLPSSSYQHMKIHKRILGHLSSVYCVAFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRGHSAEICDMAVNHENSLIASASCDKVIRVWCLRTCAPITVLQAHAASITSIQFCPAARGTTRYLASTGADGMVCFWKWHSLTMKFNDHPVKFMERSRPGVQVSTSSFSSGGMFMATGGTDHMIRVYYLGAESPMKVSEMDAHTDKVVVVQFSNNSDSLRFVSGSRDGTVKIWHYQQQEWKSTTLDMAARLPGSTAATGDDKAKLVVTMVAWDRTDSTIITAVSNYLLKVWSTSTGQLLHILSGHDDEVFVLEAHPFDSRIMLSAGHDGNIYIWDLTKGVKIRNFFNMIEGQGHGAIFDCKFSADGQHFACTDSHGHLLIFGFGCSRPYEKIPDQMFFHTDYRPLIRDSNNYVLDEQTQQAPHLMPPPFLVDVDGNPHPSHYQRLVPGRENCKEEQLIPQMGYMANNDGELVEQVLGQQTAENGESPLDNLIRQLQNEQDERQNAEREAEGEEAGEGAEAVPSSPVVAPRRSGQVDGVWQMQHNALPSQVATERDLLAWSRRVVVNEVLQGIFRVSEECRQAKGDIECSLYYAERKKKPVTCAPKSDLLSSRLRSLRPTKKTHKRHAYQTRSAQIRRPGTRSRNPSNRRTSNSQMDSESDGEQAEEQVEQTDASSDGDARWQSESSSSDSSSEYSDWTADAGINLQPPKRPTRRPVQPPDYSSSEEEGDNEAKEARTREKEKRKKKSKETKQKPTSSLGGLDAEEWLPPSWIMETVPRCSPFVPQMGDELIYFKQGHQAYVRAVRRAKAYSINPQKQPWNRLNLRDQESVKVVGIKYEVGPPTLCCLKVAFLDPVSGKMTNESFSLKYHDMPDVIDFLVLQQFYNEAKEHNWQPGMRFRSIIDDAWWFGSVEDQEPLQPEYPDSLFQCYAVKWDNGEREKMSPWDMEPIPEEAALPEQVGDGVEVAEEELKALLYEPQEGEWGAHTRDEECERVIHGIDQLLTLDVAKAFALPVNLQDYPLYCTVVAYPTDLSSIRTRLENRFYRRISALMWEVRYIEHNARTFNEPQSPIVAAAKVVTDVLLHYIGDQSCIDVLDLYRKLKSEVSSEAEVDLDVDSDTPGTSTGHRGGTANSKKRGVVLDPKAWRGQCRELLRRMTASTNSEPFRQPVDLFEYPDYRDIIDTPMDLGTVSETLAVGNYENPMEFSKDIRLIFSNSKAYTPNKKSQIYTMTLSLSAFFEKHIISIISDYKSAIQNERRARQHLTYRNRLHNGGSSPPNSPSHSSKGKHKSGKASKPKKSQTSTKNRSQRSHKAQQSGNVAEEEDVQPSSPTSGTSVRSRNQGPHRVTRSQATPVKKSGHQNNLHLSNGSRQRHLRETSQSDDEEEASGSNSSSLESSSTSSSSSSSSSSSSSSSSSDSDNSSDSEVEKYVEGGGDHDYSKAPCLSVRLSAKGKVAASEKRRHKGGEDTEAQGPKRARLQLEVEEQQDEEEEEEEEEEEEEEEEEEEEEEEEEEEEPEDEDDNNSEVQTRAAAAPAAASSQRGSQCKSRRVNTRNQGRRTVRYGDDSEDDGHGSKDDPLNLGMSRSGRVRRMTEKARVSHLMGWSH

Target ORF information:

RefSeq Version	XM_023155317.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra bromodomain and WD repeat domain containing 3 (brwd3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023155317.2

ORF Insert Sequence:

ATGGCAAGAG AACAGTGTTC ACAAATCGAA GCTGAACTCT ATTACCTCAT TGCCAGATTT 
CTGCAGTCTG GACCATGTAA AAAAGCCGCA AAGATCCTGA TCGAGGAGCT GGAGGAGTAT 
GAGTTGGTCC CGCAACGATG GAGTTGGGAC GGGAAGAAAT ACAAACGGAA CTTCCAAGAT 
TGGGTGGTGT TGAACCAGCA CATTCCTGCA GACTATTTGC TCAGGATTTG CCAGCGGATA 
GGCCCACTTA TAGAAAAGGA GATCCCACCT TGTGTTCCTG GAGTCCAGAC ACTTCTGGGG 
CTGGGAAGAC AGTCCTTGCT TCGCACCAGC AAAAGTTGTA GCCACAAGGT ATGTAGTGGC 
TCAGCTGTCG CTGCACTCCA CCGAGGACGT CCACCAGAAC CTCCAGTGAG CAATGGAAGT 
TCTCCTAATG TTGTGTCCAT CATGGGTGCT CGCCAGGCCA CAGGCACAGG TCGTTTTGGC 
CAGGTTCTGC CGTCTTCCTC CTACCAACAC ATGAAGATAC ACAAACGTAT CCTTGGCCAC 
CTTTCTTCAG TCTACTGTGT GGCCTTTGAC CGTACCGGTC GTCGAATATT TACGGGTTCT 
GATGATTGCT TGGTAAAAAT CTGGGCCACA GATGATGGCC GGCTCCTGGC AACACTCCGC 
GGTCACTCAG CAGAGATCTG TGACATGGCT GTCAACCACG AGAACAGCCT CATTGCTTCT 
GCCAGCTGTG ACAAAGTCAT CAGAGTGTGG TGCTTACGCA CCTGTGCGCC CATCACTGTG 
CTGCAGGCTC ATGCTGCGTC TATCACATCC ATACAGTTTT GTCCAGCAGC TAGAGGAACA 
ACTCGGTACC TGGCATCAAC TGGTGCAGAC GGCATGGTGT GTTTCTGGAA GTGGCATTCG 
CTCACCATGA AATTTAATGA TCATCCAGTC AAGTTTATGG AGCGTTCACG GCCCGGGGTG 
CAAGTCTCCA CTTCATCTTT CAGCAGTGGT GGCATGTTCA TGGCTACTGG AGGCACTGAT 
CATATGATCA GGGTCTACTA TCTTGGTGCT GAGTCTCCTA TGAAGGTTTC TGAGATGGAT 
GCTCACACGG ATAAAGTCGT GGTCGTCCAG TTCAGCAATA ACAGTGACAG CCTAAGGTTC 
GTGAGCGGCA GTCGCGACGG TACGGTGAAG ATTTGGCATT ACCAGCAGCA GGAGTGGAAG 
AGCACCACTT TAGACATGGC TGCAAGGCTT CCAGGGAGCA CTGCCGCCAC AGGGGACGAC 
AAAGCTAAGT TGGTGGTGAC TATGGTTGCC TGGGACCGCA CTGACAGCAC CATAATCACA 
GCGGTGTCAA ACTACCTGCT CAAAGTGTGG AGCACATCCA CAGGACAGCT GCTGCACATT 
TTGTCTGGTC ATGACGACGA GGTGTTTGTG CTGGAGGCTC ATCCATTTGA CTCCCGCATC 
ATGTTATCTG CCGGCCACGA TGGCAACATT TACATCTGGG ACCTAACCAA AGGAGTCAAG 
ATCCGCAATT TCTTCAACAT GATTGAGGGC CAAGGCCACG GTGCTATTTT TGACTGCAAG 
TTCTCAGCAG ATGGGCAGCA TTTTGCCTGC ACTGACTCAC ACGGCCATTT GCTCATCTTT 
GGTTTTGGCT GCAGCAGACC ATATGAAAAG ATTCCCGACC AGATGTTCTT CCACACGGAC 
TATCGCCCTC TTATCCGTGA CTCTAATAAC TATGTCCTGG ATGAGCAGAC GCAACAGGCG 
CCACACCTCA TGCCTCCACC CTTCCTTGTG GATGTCGACG GGAATCCTCA TCCTTCTCAC 
TACCAGCGCC TGGTGCCTGG ACGGGAGAAC TGCAAGGAAG AGCAGCTAAT ACCTCAGATG 
GGATACATGG CTAACAATGA TGGAGAGTTG GTTGAGCAGG TTCTTGGCCA GCAAACGGCA 
GAAAATGGAG AAAGCCCATT GGACAATCTC ATCAGACAGC TGCAGAATGA ACAAGATGAG 
CGTCAGAATG CAGAGCGGGA GGCAGAAGGG GAAGAAGCTG GTGAAGGCGC AGAGGCGGTG 
CCCTCCTCTC CAGTTGTTGC ACCTCGCAGA AGTGGGCAGG TGGATGGGGT GTGGCAAATG 
CAGCATAACG CCCTTCCCAG TCAGGTGGCC ACCGAGAGGG ACCTGCTGGC CTGGAGCCGC 
AGAGTGGTGG TCAATGAAGT GCTGCAGGGG ATATTCAGAG TTTCAGAGGA GTGCAGACAA 
GCCAAAGGTG ACATTGAATG TTCTCTATAC TACGCTGAGA GGAAGAAGAA ACCAGTAACC 
TGTGCACCTA AGAGCGATCT CTTGTCTTCA CGCCTGCGCT CCCTGCGGCC CACGAAGAAA 
ACTCATAAGC GCCATGCCTA TCAAACCCGG TCAGCTCAAA TCCGGCGTCC TGGAACCAGG 
AGTAGAAATC CCAGCAACCG ACGTACCTCT AACAGCCAGA TGGACTCGGA GAGTGATGGA 
GAACAGGCAG AAGAACAGGT GGAGCAAACC GATGCATCTT CAGATGGTGA TGCTCGGTGG 
CAGAGTGAAA GCAGCTCCAG CGATTCCTCC AGTGAATATT CTGATTGGAC AGCGGATGCT 
GGGATCAATT TGCAGCCGCC AAAGCGACCT ACCAGGAGAC CTGTACAGCC TCCAGATTAC 
AGCAGCTCTG AGGAAGAAGG AGATAACGAA GCCAAGGAGG CAAGGACGAG GGAAAAAGAG 
AAGAGGAAGA AGAAGTCCAA GGAGACCAAA CAAAAACCCA CCTCCTCTCT GGGTGGACTT 
GATGCGGAAG AGTGGCTGCC GCCATCTTGG ATTATGGAGA CTGTCCCCCG CTGCTCTCCT 
TTTGTTCCAC AAATGGGAGA TGAACTCATC TACTTCAAAC AGGGCCATCA GGCTTACGTC 
CGGGCTGTTC GCAGAGCTAA GGCTTACAGC ATCAACCCTC AGAAGCAGCC GTGGAACAGA 
CTCAACCTCA GGGATCAGGA ATCTGTGAAA GTGGTTGGAA TTAAGTATGA AGTGGGACCT 
CCTACCCTCT GCTGCTTAAA AGTGGCGTTC TTGGACCCAG TCTCAGGGAA AATGACCAAT 
GAGTCTTTCT CCTTAAAGTA TCATGACATG CCTGATGTCA TTGATTTTCT GGTACTGCAG 
CAGTTTTACA ACGAGGCTAA AGAGCACAAC TGGCAACCTG GCATGAGGTT CCGCAGCATC 
ATTGACGACG CCTGGTGGTT CGGCTCTGTG GAGGATCAAG AGCCCCTGCA GCCAGAGTAT 
CCCGACAGCC TGTTTCAGTG CTACGCAGTA AAATGGGATA ACGGTGAGCG GGAGAAAATG 
AGTCCCTGGG ATATGGAGCC TATTCCTGAG GAAGCTGCTT TGCCAGAGCA GGTTGGTGAT 
GGTGTAGAGG TGGCAGAAGA GGAGCTGAAA GCTCTACTCT ATGAGCCTCA GGAAGGTGAA 
TGGGGGGCTC ATACACGAGA TGAAGAATGT GAGCGAGTCA TTCATGGCAT CGATCAGCTT 
CTGACACTGG ATGTAGCCAA GGCGTTCGCA TTACCGGTAA ATTTGCAGGA CTACCCTCTG 
TACTGCACCG TAGTGGCTTA CCCCACTGAC CTCAGCTCCA TCCGCACACG TCTGGAGAAT 
CGCTTTTACA GGCGGATCTC TGCATTAATG TGGGAGGTGC GTTACATCGA ACACAATGCC 
CGCACTTTCA ATGAACCTCA AAGCCCCATT GTAGCAGCTG CCAAGGTGGT GACTGATGTT 
CTCCTTCATT ATATCGGGGA CCAAAGTTGC ATCGATGTCT TGGATCTGTA TCGTAAACTG 
AAGTCTGAAG TCAGCAGTGA AGCTGAGGTT GACTTGGACG TAGATTCAGA CACTCCAGGA 
ACATCTACAG GACACCGGGG AGGCACTGCA AATTCTAAGA AGCGTGGTGT GGTTCTGGAT 
CCGAAAGCCT GGCGAGGTCA ATGTCGAGAG CTGTTGCGTC GAATGACAGC TTCCACGAAT 
TCCGAACCTT TCAGGCAGCC TGTGGATCTC TTTGAATATC CGGACTATCG AGACATCATT 
GACACTCCCA TGGATCTGGG TACAGTGTCA GAGACCCTGG CAGTAGGAAA TTATGAAAAT 
CCCATGGAGT TTTCCAAAGA CATCCGCCTC ATTTTCAGCA ACTCTAAAGC ATACACTCCT 
AACAAGAAGT CACAGATCTA TACCATGACC CTCAGCTTGT CGGCCTTCTT TGAAAAACAC 
ATCATCTCCA TCATTTCTGA CTACAAGTCT GCCATTCAGA ACGAACGGCG GGCTCGTCAA 
CATCTCACTT ACAGGAACAG ATTGCACAAC GGAGGCAGCT CGCCACCTAA TAGTCCATCC 
CACAGCTCAA AAGGAAAGCA CAAGTCAGGG AAGGCATCAA AGCCAAAAAA ATCCCAAACC 
TCAACAAAGA ATCGTTCTCA GAGATCACAT AAAGCTCAGC AAAGCGGTAA TGTAGCAGAG 
GAAGAGGACG TCCAGCCTTC TTCCCCAACT TCAGGGACGA GTGTCAGGAG CAGAAATCAA 
GGACCACATC GGGTGACCCG CAGCCAAGCA ACTCCCGTGA AGAAATCAGG TCACCAGAAT 
AACTTGCACC TAAGTAACGG CTCCAGACAA CGGCACTTGC GAGAAACGTC GCAGTCCGAC 
GATGAAGAGG AGGCGTCAGG ATCCAACAGC TCCTCGTTGG AGTCGTCCTC CACCTCGTCG 
TCATCATCAT CATCATCATC ATCCTCCTCC TCCTCCTCAT CGTCGGACAG CGACAACAGC 
TCTGACTCTG AGGTGGAAAA GTACGTGGAG GGAGGAGGAG ATCACGACTA CAGCAAAGCC 
CCCTGCCTGT CAGTACGCCT CTCAGCTAAG GGTAAGGTGG CAGCCTCAGA AAAGAGGAGA 
CACAAAGGAG GAGAGGACAC AGAGGCACAG GGACCTAAAC GAGCACGACT GCAGCTTGAG 
GTAGAGGAGC AGCAGGATGA GGAGGAGGAG GAGGAAGAGG AGGAAGAAGA AGAAGAAGAG 
GAGGAAGAAG AAGAAGAGGA GGAAGAAGAG GAAGAGGAGG AGCCAGAGGA CGAGGATGAT 
AACAACTCTG AGGTGCAGAC ACGAGCAGCA GCAGCTCCAG CAGCAGCTAG CAGCCAGAGA 
GGCAGCCAGT GTAAGTCTCG GCGGGTCAAC ACACGAAACC AGGGCCGCAG GACAGTTCGA 
TACGGAGACG ACTCGGAGGA TGATGGCCAT GGGTCGAAAG ACGACCCTCT AAACCTGGGC 
ATGTCCCGCT CAGGACGGGT ACGCCGTATG ACTGAGAAAG CTCGTGTCAG CCACCTTATG 
GGCTGGAGTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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brwd3 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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