prrc2b cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	prrc2b
Entrez Gene ID	101464972
Full Name	proline rich coiled-coil 2B
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014413536.1	XP_014269022.1	protein PRRC2B isoform X1
XM_023155191.1	XP_023010959.1	protein PRRC2B isoform X3
XM_014413536.2	XP_014269022.1	protein PRRC2B isoform X1
XM_023155192.1	XP_023010960.1	protein PRRC2B isoform X4
XM_014413537.1	XP_014269023.1	protein PRRC2B isoform X2
XM_014413537.2	XP_014269023.1	protein PRRC2B isoform X2
XM_023155191.2	XP_023010959.1	protein PRRC2B isoform X3

Pathways from BioSystems
Gene Ontology

The following prrc2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prrc2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa25188	XM_014413536.1 Latest version!	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa25189	XM_014413537.1 Latest version!	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa311653	XM_023155191.1 Latest version!	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa311654	XM_023155192.1 Latest version!	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa374705	XM_014413536.2 Latest version!	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa374706	XM_014413537.2 Latest version!	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa374707	XM_023155191.2 Latest version!	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prrc2b ( XM_014413536.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014269022.1 Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa25188
Clone ID Related Accession (Same CDS sequence)	XM_014413536.1
Accession Version	XM_014413536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447383.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAACC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCACA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGAGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGCAG GAGTCCAGAT 
GAGTCAGGCT CTGTATACAG CATAGAGCAG CTGCCAGAAG AGAGGGAGCC CATTGAGCCC 
GCTGGGAAGA AATTAGACAA GGAGCTGAAG CCAGGACCTG TCAAAACAGA CATCACTGAA 
CCACTGTCCC CATATGAGCT CAGCAGCTAC CCAATTGAAG GAGACGCAGG GGGACCAGGT 
TCAAATGCAG ATGGATACCA GGATGCCTTG TCTAAAAAGC AAAGACGCCC ACAGGAAGAT 
GACAGGAGGA GGAAAGAACA GGGGGCAGCT GTACCAGTGA AAAACAGGAC AGTCACTTCC 
AAGATGCCAC CACGCTTTGC CAAAAAGCAG GGAAGCATGA GCATTGAACA ACCCGAGGAA 
GGAATTTCTT CTAACAATCT GGGAACTGAA ATCTGGGAGA CCAACAGCTC AGCTCTTTCA 
GTGCAGTCTT CAGGGGGAGA CTCATGGACC AAACAGGTGT CTTACACTGG GAGTGAGCCC 
AACTCTGAGG ACTCTGATGC GGGCCCAGAA CAAAACAAAG AGCAGCACAA ACCAGGGCCC 
ATCGGAAATG AACGCTCCCT GAAACACCGG AAGGGCTCAG AAGGTGTCGA TAGGCTGGAA 
GGTGGCCCTA TCACACCAGT CAATGGTGTG GACCTCCATG TGGACACTGT GCTGCCTGTC 
CCACCCATTG AGTTTGGTGT CAGTGCCAAA GATTCAGATT TCAGCCTGCC GCCGGGTTCT 
ACCCCAGTAC CCGTGTCCAA TCCTGTGAAC AAGCTTCAGG ACACTGCTTT GAATCAGAGT 
ATCCCCATTT TGCGCTCCAA CCACCTGCAG CCTGGCATCA ACCTCAACCC CATCTCCTTT 
CCCAGTGCTG ATCTCACTCT CAAGATGGAG TCAGCACGCA AGGCGTGGGA GAACTCCCAG 
TCTCTTCCTG AGCAGGGCTC TCCTGGTGGC AGCGCTTCAG GTGCTCAGCC TCCCTGCAGT 
GCCGGCTCAT CCACTGGTGT CAGTTACAGC TCGTTTGGAG GCGTTTCCAT GCCACCAATG 
CCCGTTGCAT CAGTAGCACC TTCCATGTCC ATGCAAGGGA ATCATATTCC CCCGCTGTAT 
CTGGATGGTC ACGTCTTTCC AAGCCAGCCA CGCCTTGTAC CACCTACCAT GACCCAGCAG 
CAGACTTACC AACAGGCGGC TGCAGCCCAG CAGATTCCCA TTTCTTTACA CACGTCTCTT 
CAGGCTCAGG CCCAGTTGGG GCTTCGGGGA GGTCTGCCTG TTTCTCAGTC CCAAGAGATG 
TTCAGCTCCA TCCCCCCCTT CAGGTCCCAG GTTTACATGC ACCCCAACTT GTCACAGCCC 
AGCCCCATGG TACTGTCAGG TGGAGCCCCT CTCAAGGGCC CATACTCAGC TTTCCCTGGC 
ATGCAGCCAT CAGACATGGT TAAACCCCAG TCAGGCTCAC ACTACCAGCC TATGAATGGC 
AGCCAGCAGC TAGTCTATGA TAGTCAGATG AACCAAGGGC CTGGCATGGG CTCTTCACAG 
TTAATGGACT CTCAGCTCAT TCAGGTCACT ATGCCCCTGC CTGGCTCTCA GCTGCGTTAT 
GGTTCAGCTC AACAACACCT CATCCTCCCA CAGTCCATCC AGCTGCAGCA GGGACAGAAC 
TTGTCAGTGG GAGCCCCACG CCGAATGCTA CCACCAGGGT CGCAGGCAGC TGTCATGACT 
GGCAGCAGAG AGGGTCCACA GATGGAAATG AAAGGTTTTC AGTTCTCTGA GAAGCCCAAT 
CATTCCCCAG GCATGTCTGG AGGTTCTTAC AGACCTGGCT CTGCTAGCCC CGGCGGGAAG 
CCCTCTGGTC CTGGAGGACC TGTAGGCCCT CTGCCTACAC ATTTTGCTCA GCAGGTCCCT 
CCTGCTCAGG GCAGCATGGT GATGCACATG CGTCCCCCCA CCACAGGCCC CTTCCCCAAC 
CCTATTCAGA GACCTGTTAT GCAGGTCAAT AAGCCCGTCA TCATCCGTTC CCCCCCTTAC 
CCCAATCCTG GCCGTGACCC TCCCCACTCC ATCCCTCCTT CAGCTTCCGA GCCCCCAGTT 
AAAGGGCAAG AGGATGGCAT GAAGAATAAA ACAATAAGAG ATGTACGCAA GGCGGTGGGA 
GAGGGCAAAA CACCGTCCGG GGGCATGACC AGCAAACTCC AGGAGCCCCT ACCTTCCACA 
GGCCAAGCCA AACCAGCACG CACTGGAGCC ATCAAACCCC AGGCTGTCAA AGTAGAAGAG 
GGCAAGGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014269022.1
CDS	232..6903
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKNTVVPRHGLQSLGKVATARRMPPPAHLPSLKSENKGNDPNVIIVPKDGTGWANKQEQPDQKNSVASTPQLPELQPPLPLQKSVSNLQKSSPVANQENTNTSGPKQWAQLNGKAVEQDGSRASNRLQPFSHEEFPTLKAAGEQDRAGKERSGFDPSYGPGPSLRPQNVTSWREGGGRNLQPSSLTLSLPADPEGKATALAETGTPPASSHPSSATVTTSSNTVTAPSPGPDPKELSLRPAQPVRRTAVPTALQYQLHHTSTAVYHDMLPAFMCSKETREATGTDHVPTTVAAPARFDSKPTFRQSYAKPELVNGDVRRENRFVRAAPRLSSQPIRRPTDRPVRPAIINPEDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEEHSSSDKNKIWADWERERDNQRDCHSSLSSGEASYPQEGPEESYSFQHHHHEPPRKTNGKYLSTDTQVQQRNQGDPLADQDDHQRQSQAPARAKYVSPELSEAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTERQTSRTDDSQKEVEGKEVPLSPSREQSRSHHDNWHYSTKDGSECPSDISPGHSYREESGFSTYRGSEDDGPEPSSPSGDYSGRQPIKPVPPRFQKQPQHHQQQEQMFKMQHWQQSGHPNPSGSSHTQRGYYPPHVLGFDPRWMMMPPFMDPRMTQGRSPVDYYPAGMIKPMMHQDHLNSPGSDEGCHPNLPQERRAPSTEPYPVWNQDGYPLRSFTPPYQRQRESSESGHPDDRGDMASSQQDSYEERASDCLSHTQDDLPHHSYQSRGSDREQHDQGLLTTAQNLSQNYSDSEYPKQESRDKHLKDGLESHDEALDGSTDNWKRDGSQKQDGGVNSAQNQWSEASSSSSSVNQPSEASGRTLIRRTGPIKKPVLKALKVEDKENEKPKPEPEEKPVPYRLEKEVLTNVYDLKKDNQPANNRRSASPVVEKQPEERQRQSPAPSKMDRPLSTHSDDSPKENTWSSAKSQSLRDSQENREPQAPRRNNWIFIDEEQAFGAVRGTGRGRSRGFREFNSRGGTRGGRGGDNLRGVYNNNNNSSGSSTQRAGRGRAPPRDLIKVEEFQRGKPRRRNVSETLSEASEYEELPKRRRQKGSENGEGYTESGDVRKADRESWRSNKVYTDDQTAPDSREKTKVTRGFGNRVLPPRLNTTGSYSRSFGGSRDISTWRGRGPQFSGSSSGSMQENGYGPGAETAYSRRPPVERDALKYTPKFTGSFMENGTEEREGEYYFDSENPDRQILRRRRPPRQDKPPRFRRLRQEREPGSNQWTSDEFINGDFANPWPGHSKSTGDDNWPSGHYSGRPSQHGQTEEWETGSDNSDFGDWREKRSGGGGIATQGHGDIPSDSGQEPGSSEKRELSKRSFSSQRPLVERQNRKGEPSLLDASKMVRAPDNPPTSSNRSDSWQNGGTSCKSRSPDESGSVYSIEQLPEEREPIEPAGKKLDKELKPGPVKTDITEPLSPYELSSYPIEGDAGGPGSNADGYQDALSKKQRRPQEDDRRRKEQGAAVPVKNRTVTSKMPPRFAKKQGSMSIEQPEEGISSNNLGTEIWETNSSALSVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQNKEQHKPGPIGNERSLKHRKGSEGVDRLEGGPITPVNGVDLHVDTVLPVPPIEFGVSAKDSDFSLPPGSTPVPVSNPVNKLQDTALNQSIPILRSNHLQPGINLNPISFPSADLTLKMESARKAWENSQSLPEQGSPGGSASGAQPPCSAGSSTGVSYSSFGGVSMPPMPVASVAPSMSMQGNHIPPLYLDGHVFPSQPRLVPPTMTQQQTYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEMFSSIPPFRSQVYMHPNLSQPSPMVLSGGAPLKGPYSAFPGMQPSDMVKPQSGSHYQPMNGSQQLVYDSQMNQGPGMGSSQLMDSQLIQVTMPLPGSQLRYGSAQQHLILPQSIQLQQGQNLSVGAPRRMLPPGSQAAVMTGSREGPQMEMKGFQFSEKPNHSPGMSGGSYRPGSASPGGKPSGPGGPVGPLPTHFAQQVPPAQGSMVMHMRPPTTGPFPNPIQRPVMQVNKPVIIRSPPYPNPGRDPPHSIPPSASEPPVKGQEDGMKNKTIRDVRKAVGEGKTPSGGMTSKLQEPLPSTGQAKPARTGAIKPQAVKVEEGKA

Target ORF information:

RefSeq Version	XM_014413536.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014413536.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAACC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCACA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGAGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGCAG GAGTCCAGAT 
GAGTCAGGCT CTGTATACAG CATAGAGCAG CTGCCAGAAG AGAGGGAGCC CATTGAGCCC 
GCTGGGAAGA AATTAGACAA GGAGCTGAAG CCAGGACCTG TCAAAACAGA CATCACTGAA 
CCACTGTCCC CATATGAGCT CAGCAGCTAC CCAATTGAAG GAGACGCAGG GGGACCAGGT 
TCAAATGCAG ATGGATACCA GGATGCCTTG TCTAAAAAGC AAAGACGCCC ACAGGAAGAT 
GACAGGAGGA GGAAAGAACA GGGGGCAGCT GTACCAGTGA AAAACAGGAC AGTCACTTCC 
AAGATGCCAC CACGCTTTGC CAAAAAGCAG GGAAGCATGA GCATTGAACA ACCCGAGGAA 
GGAATTTCTT CTAACAATCT GGGAACTGAA ATCTGGGAGA CCAACAGCTC AGCTCTTTCA 
GTGCAGTCTT CAGGGGGAGA CTCATGGACC AAACAGGTGT CTTACACTGG GAGTGAGCCC 
AACTCTGAGG ACTCTGATGC GGGCCCAGAA CAAAACAAAG AGCAGCACAA ACCAGGGCCC 
ATCGGAAATG AACGCTCCCT GAAACACCGG AAGGGCTCAG AAGGTGTCGA TAGGCTGGAA 
GGTGGCCCTA TCACACCAGT CAATGGTGTG GACCTCCATG TGGACACTGT GCTGCCTGTC 
CCACCCATTG AGTTTGGTGT CAGTGCCAAA GATTCAGATT TCAGCCTGCC GCCGGGTTCT 
ACCCCAGTAC CCGTGTCCAA TCCTGTGAAC AAGCTTCAGG ACACTGCTTT GAATCAGAGT 
ATCCCCATTT TGCGCTCCAA CCACCTGCAG CCTGGCATCA ACCTCAACCC CATCTCCTTT 
CCCAGTGCTG ATCTCACTCT CAAGATGGAG TCAGCACGCA AGGCGTGGGA GAACTCCCAG 
TCTCTTCCTG AGCAGGGCTC TCCTGGTGGC AGCGCTTCAG GTGCTCAGCC TCCCTGCAGT 
GCCGGCTCAT CCACTGGTGT CAGTTACAGC TCGTTTGGAG GCGTTTCCAT GCCACCAATG 
CCCGTTGCAT CAGTAGCACC TTCCATGTCC ATGCAAGGGA ATCATATTCC CCCGCTGTAT 
CTGGATGGTC ACGTCTTTCC AAGCCAGCCA CGCCTTGTAC CACCTACCAT GACCCAGCAG 
CAGACTTACC AACAGGCGGC TGCAGCCCAG CAGATTCCCA TTTCTTTACA CACGTCTCTT 
CAGGCTCAGG CCCAGTTGGG GCTTCGGGGA GGTCTGCCTG TTTCTCAGTC CCAAGAGATG 
TTCAGCTCCA TCCCCCCCTT CAGGTCCCAG GTTTACATGC ACCCCAACTT GTCACAGCCC 
AGCCCCATGG TACTGTCAGG TGGAGCCCCT CTCAAGGGCC CATACTCAGC TTTCCCTGGC 
ATGCAGCCAT CAGACATGGT TAAACCCCAG TCAGGCTCAC ACTACCAGCC TATGAATGGC 
AGCCAGCAGC TAGTCTATGA TAGTCAGATG AACCAAGGGC CTGGCATGGG CTCTTCACAG 
TTAATGGACT CTCAGCTCAT TCAGGTCACT ATGCCCCTGC CTGGCTCTCA GCTGCGTTAT 
GGTTCAGCTC AACAACACCT CATCCTCCCA CAGTCCATCC AGCTGCAGCA GGGACAGAAC 
TTGTCAGTGG GAGCCCCACG CCGAATGCTA CCACCAGGGT CGCAGGCAGC TGTCATGACT 
GGCAGCAGAG AGGGTCCACA GATGGAAATG AAAGGTTTTC AGTTCTCTGA GAAGCCCAAT 
CATTCCCCAG GCATGTCTGG AGGTTCTTAC AGACCTGGCT CTGCTAGCCC CGGCGGGAAG 
CCCTCTGGTC CTGGAGGACC TGTAGGCCCT CTGCCTACAC ATTTTGCTCA GCAGGTCCCT 
CCTGCTCAGG GCAGCATGGT GATGCACATG CGTCCCCCCA CCACAGGCCC CTTCCCCAAC 
CCTATTCAGA GACCTGTTAT GCAGGTCAAT AAGCCCGTCA TCATCCGTTC CCCCCCTTAC 
CCCAATCCTG GCCGTGACCC TCCCCACTCC ATCCCTCCTT CAGCTTCCGA GCCCCCAGTT 
AAAGGGCAAG AGGATGGCAT GAAGAATAAA ACAATAAGAG ATGTACGCAA GGCGGTGGGA 
GAGGGCAAAA CACCGTCCGG GGGCATGACC AGCAAACTCC AGGAGCCCCT ACCTTCCACA 
GGCCAAGCCA AACCAGCACG CACTGGAGCC ATCAAACCCC AGGCTGTCAA AGTAGAAGAG 
GGCAAGGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_014413537.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014269023.1 Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

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CloneID	OMa25189
Clone ID Related Accession (Same CDS sequence)	XM_014413537.1
Accession Version	XM_014413537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447383.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTTCCG 
CGACATGGCT TGCAGAGTCT TGGTAAAGTG GCCACAGCCC GGCGTATGCC CCCACCAGCT 
CATCTGCCGA GCTTGAAGTC TGAAAACAAA GGAAACGATC CCAACGTGAT TATAGTGCCC 
AAAGACGGTA CAGGATGGGC GAACAAGCAG GAACAACCCG ATCAAAAGAA TTCTGTTGCA 
TCAACACCAC AGCTGCCGGA GTTGCAGCCG CCGCTGCCTT TGCAGAAATC TGTCTCCAAT 
CTTCAGAAGT CCTCACCGGT AGCCAACCAG GAGAACACAA ACACAAGTGG ACCAAAGCAA 
TGGGCCCAGC TAAATGGAAA GGCAGTAGAG CAAGATGGTT CAAGGGCCTC AAACCGACTT 
CAGCCCTTCT CTCACGAGGA ATTTCCCACA CTGAAGGCAG CTGGAGAACA GGACAGGGCT 
GGCAAGGAAA GAAGCGGCTT CGATCCGTCG TATGGGCCCG GACCAAGCCT CCGCCCCCAG 
AATGTGACAA GCTGGAGGGA AGGTGGTGGC AGGAACCTTC AGCCCTCATC CCTGACCCTC 
AGCCTGCCAG CAGATCCTGA GGGTAAGGCC ACTGCCCTGG CTGAGACTGG CACCCCTCCA 
GCCTCGTCTC ACCCCTCCTC CGCCACAGTC ACCACCTCAT CTAATACGGT GACGGCTCCG 
TCACCAGGCC CTGACCCCAA GGAGCTTTCC CTGCGACCTG CTCAGCCTGT CCGCAGAACA 
GCCGTCCCTA CTGCATTGCA GTACCAGCTT CATCACACTT CAACTGCTGT CTACCATGAC 
ATGTTGCCTG CCTTTATGTG CTCTAAAGAG ACACGTGAAG CTACGGGTAC AGACCATGTT 
CCCACCACCG TAGCAGCCCC CGCCCGTTTT GACAGCAAAC CTACTTTTAG GCAGAGCTAT 
GCCAAACCTG AGCTTGTAAA TGGTGACGTA AGGAGAGAGA ATCGCTTTGT CCGTGCTGCG 
CCTCGACTTT CTTCACAGCC TATCCGTAGA CCTACCGACA GACCAGTTCG CCCAGCCATT 
ATTAATCCAG AGGATTTGAA GGATCTGGAT GAGCTTGACA ATGATTGTGA AGATGGATGG 
GCTGGACTTC ATGAGGAGGT TGATTATAGT GAGAAGCTCA AATTTAGTGA TGATGAAGAA 
GAACACTCTT CCAGTGACAA AAACAAGATC TGGGCTGACT GGGAGAGGGA GAGAGATAAC 
CAGCGGGACT GCCATTCCTC CCTTAGCTCG GGGGAGGCAT CTTACCCACA AGAGGGTCCA 
GAAGAGAGTT ACTCTTTCCA GCATCACCAC CACGAGCCTC CCAGGAAGAC CAACGGCAAA 
TATCTCTCCA CAGACACCCA GGTCCAACAG AGAAACCAAG GAGACCCACT TGCTGACCAG 
GATGATCACC AGCGGCAGTC TCAGGCACCA GCTAGGGCCA AGTATGTGTC GCCTGAGCTG 
TCTGAGGCTG TTGAGAGAGC CCGAAGACGG CGGGAGGAGG AAGAGAGGCG TGCTCGTGAG 
GAACGTCTGG CAGCATGTGC TGAAAAGCTC AAAAAACTGG ATGAAAAATT TGGGAAGACT 
GAAAGGCAGA CATCAAGGAC GGATGACAGC CAGAAGGAGG TAGAGGGAAA AGAAGTTCCA 
CTGTCCCCAA GTCGGGAACA GAGTCGAAGC CACCATGATA ACTGGCACTA CAGCACAAAA 
GATGGAAGTG AGTGTCCCTC GGACATTTCC CCTGGCCATA GTTACCGTGA AGAGTCTGGC 
TTCTCTACCT ACCGTGGCAG TGAAGACGAT GGCCCAGAGC CCTCCTCACC ATCAGGAGAC 
TACAGTGGGC GTCAGCCAAT CAAACCAGTG CCACCCCGCT TTCAAAAGCA ACCACAACAC 
CACCAGCAGC AGGAACAAAT GTTCAAGATG CAGCATTGGC AGCAGTCAGG TCACCCTAAT 
CCATCAGGGT CAAGCCACAC TCAGCGGGGC TACTACCCTC CACATGTCCT CGGTTTTGAT 
CCGCGCTGGA TGATGATGCC ACCTTTCATG GATCCCCGCA TGACCCAGGG ACGGTCTCCT 
GTTGACTACT ACCCTGCAGG AATGATAAAA CCTATGATGC ACCAAGATCA CTTGAACAGT 
CCCGGTTCTG ATGAGGGATG CCATCCAAAC TTGCCTCAGG AGAGACGAGC GCCTTCCACT 
GAGCCTTACC CAGTGTGGAA TCAAGATGGC TACCCCTTGC GTAGCTTTAC TCCACCTTAC 
CAGAGACAGC GTGAAAGCTC GGAAAGCGGA CATCCCGATG ACAGAGGTGA TATGGCCTCT 
TCCCAACAGG ACTCCTATGA AGAGAGGGCC AGTGATTGTT TGTCCCACAC TCAAGATGAT 
CTTCCCCATC ACAGTTATCA GAGCAGAGGT TCAGACAGAG AACAACATGA CCAAGGCTTG 
CTCACCACCG CTCAGAACCT CTCTCAGAAT TATTCAGATA GTGAATACCC AAAGCAAGAG 
TCGAGAGACA AGCATCTGAA AGATGGCCTT GAATCCCACG ACGAGGCCTT AGATGGCTCC 
ACAGACAACT GGAAAAGAGA TGGTAGCCAG AAACAAGACG GAGGGGTTAA CAGTGCCCAA 
AACCAGTGGT CTGAAGCCAG TTCCAGTTCA AGTAGTGTCA ACCAGCCATC TGAGGCTAGT 
GGACGCACCT TGATACGCAG AACTGGTCCC ATCAAGAAAC CAGTTCTTAA GGCTCTCAAA 
GTGGAAGACA AGGAGAATGA AAAGCCTAAA CCTGAGCCTG AGGAGAAGCC TGTCCCCTAC 
CGCCTGGAGA AAGAAGTCCT TACTAACGTT TATGACTTGA AGAAAGACAA CCAGCCTGCC 
AACAATAGGC GTTCTGCATC ACCTGTGGTT GAAAAACAAC CTGAAGAGAG GCAGCGCCAG 
TCTCCAGCTC CCAGTAAAAT GGACAGACCT CTGAGCACCC ACAGCGATGA CTCTCCCAAG 
GAGAACACTT GGAGCAGTGC TAAAAGCCAG TCACTTAGAG ACAGTCAGGA GAACCGAGAA 
CCCCAGGCAC CACGGCGCAA TAACTGGATC TTTATTGATG AAGAACAGGC CTTTGGTGCA 
GTGAGGGGGA CGGGTAGAGG CCGCAGTCGA GGCTTCCGGG AATTTAACTC CAGAGGTGGA 
ACCCGCGGTG GCCGCGGTGG AGATAATCTC AGAGGTGTTT ATAACAACAA TAACAACAGC 
AGTGGCAGCA GCACTCAGCG AGCAGGCAGA GGTCGAGCAC CACCCAGGGA TCTAATCAAG 
GTCGAGGAGT TTCAGAGGGG CAAACCCCGT AGGCGAAATG TCAGTGAAAC ATTGAGTGAA 
GCCTCAGAGT ATGAGGAACT TCCCAAGAGG CGTCGCCAGA AAGGATCTGA AAATGGAGAA 
GGCTATACAG AGTCTGGGGA TGTCCGTAAA GCTGATAGAG AGTCTTGGAG ATCCAACAAG 
GTGTACACAG ATGATCAGAC TGCCCCAGAT TCCAGAGAAA AGACCAAGGT CACCAGAGGC 
TTTGGAAATC GGGTGCTGCC TCCACGACTG AACACCACTG GTAGTTACAG TCGAAGCTTT 
GGAGGATCTA GAGATATTTC TACATGGAGG GGCCGTGGGC CTCAATTTAG TGGCAGCAGT 
AGCGGCTCGA TGCAAGAAAA TGGTTATGGT CCTGGAGCAG AGACTGCTTA CTCCCGCAGA 
CCCCCAGTTG AGCGTGACGC TCTCAAGTAC ACTCCTAAAT TTACTGGCTC CTTCATGGAA 
AATGGAACAG AGGAGCGTGA AGGAGAATAC TACTTTGACA GCGAGAACCC TGACAGACAG 
ATTTTAAGGA GAAGGCGTCC TCCACGTCAA GACAAGCCTC CGCGCTTCCG TCGTCTACGA 
CAAGAGCGTG AACCTGGCTC AAATCAGTGG ACAAGTGATG AGTTCATAAA CGGAGACTTT 
GCAAATCCCT GGCCAGGTCA TTCTAAAAGC ACTGGTGATG ATAACTGGCC CAGTGGCCAC 
TACTCTGGAC GTCCTAGCCA GCATGGCCAG ACAGAGGAAT GGGAGACGGG TTCAGACAAC 
AGCGACTTTG GTGACTGGAG AGAGAAACGG AGTGGAGGTG GAGGCATTGC TACACAGGGC 
CATGGCGATA TTCCCTCAGA CTCTGGCCAG GAGCCAGGCT CCAGTGAGAA GAGGGAGCTT 
TCTAAGAGAA GCTTCTCTAG TCAGAGACCA TTGGTGGAAC GGCAGAACAG GAAAGGAGAA 
CCATCACTGC TGGACGCAAG CAAGATGGTG CGTGCACCTG ATAATCCCCC CACCTCATCG 
AACAGGAGTG ACAGCTGGCA GAACGGAGGC ACTTCTTGTA AAAGCAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014269023.1
CDS	232..6900
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKNTVPRHGLQSLGKVATARRMPPPAHLPSLKSENKGNDPNVIIVPKDGTGWANKQEQPDQKNSVASTPQLPELQPPLPLQKSVSNLQKSSPVANQENTNTSGPKQWAQLNGKAVEQDGSRASNRLQPFSHEEFPTLKAAGEQDRAGKERSGFDPSYGPGPSLRPQNVTSWREGGGRNLQPSSLTLSLPADPEGKATALAETGTPPASSHPSSATVTTSSNTVTAPSPGPDPKELSLRPAQPVRRTAVPTALQYQLHHTSTAVYHDMLPAFMCSKETREATGTDHVPTTVAAPARFDSKPTFRQSYAKPELVNGDVRRENRFVRAAPRLSSQPIRRPTDRPVRPAIINPEDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEEHSSSDKNKIWADWERERDNQRDCHSSLSSGEASYPQEGPEESYSFQHHHHEPPRKTNGKYLSTDTQVQQRNQGDPLADQDDHQRQSQAPARAKYVSPELSEAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTERQTSRTDDSQKEVEGKEVPLSPSREQSRSHHDNWHYSTKDGSECPSDISPGHSYREESGFSTYRGSEDDGPEPSSPSGDYSGRQPIKPVPPRFQKQPQHHQQQEQMFKMQHWQQSGHPNPSGSSHTQRGYYPPHVLGFDPRWMMMPPFMDPRMTQGRSPVDYYPAGMIKPMMHQDHLNSPGSDEGCHPNLPQERRAPSTEPYPVWNQDGYPLRSFTPPYQRQRESSESGHPDDRGDMASSQQDSYEERASDCLSHTQDDLPHHSYQSRGSDREQHDQGLLTTAQNLSQNYSDSEYPKQESRDKHLKDGLESHDEALDGSTDNWKRDGSQKQDGGVNSAQNQWSEASSSSSSVNQPSEASGRTLIRRTGPIKKPVLKALKVEDKENEKPKPEPEEKPVPYRLEKEVLTNVYDLKKDNQPANNRRSASPVVEKQPEERQRQSPAPSKMDRPLSTHSDDSPKENTWSSAKSQSLRDSQENREPQAPRRNNWIFIDEEQAFGAVRGTGRGRSRGFREFNSRGGTRGGRGGDNLRGVYNNNNNSSGSSTQRAGRGRAPPRDLIKVEEFQRGKPRRRNVSETLSEASEYEELPKRRRQKGSENGEGYTESGDVRKADRESWRSNKVYTDDQTAPDSREKTKVTRGFGNRVLPPRLNTTGSYSRSFGGSRDISTWRGRGPQFSGSSSGSMQENGYGPGAETAYSRRPPVERDALKYTPKFTGSFMENGTEEREGEYYFDSENPDRQILRRRRPPRQDKPPRFRRLRQEREPGSNQWTSDEFINGDFANPWPGHSKSTGDDNWPSGHYSGRPSQHGQTEEWETGSDNSDFGDWREKRSGGGGIATQGHGDIPSDSGQEPGSSEKRELSKRSFSSQRPLVERQNRKGEPSLLDASKMVRAPDNPPTSSNRSDSWQNGGTSCKSRSPDESGSVYSIEQLPEEREPIEPAGKKLDKELKPGPVKTDITEPLSPYELSSYPIEGDAGGPGSNADGYQDALSKKQRRPQEDDRRRKEQGAAVPVKNRTVTSKMPPRFAKKQGSMSIEQPEEGISSNNLGTEIWETNSSALSVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQNKEQHKPGPIGNERSLKHRKGSEGVDRLEGGPITPVNGVDLHVDTVLPVPPIEFGVSAKDSDFSLPPGSTPVPVSNPVNKLQDTALNQSIPILRSNHLQPGINLNPISFPSADLTLKMESARKAWENSQSLPEQGSPGGSASGAQPPCSAGSSTGVSYSSFGGVSMPPMPVASVAPSMSMQGNHIPPLYLDGHVFPSQPRLVPPTMTQQQTYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEMFSSIPPFRSQVYMHPNLSQPSPMVLSGGAPLKGPYSAFPGMQPSDMVKPQSGSHYQPMNGSQQLVYDSQMNQGPGMGSSQLMDSQLIQVTMPLPGSQLRYGSAQQHLILPQSIQLQQGQNLSVGAPRRMLPPGSQAAVMTGSREGPQMEMKGFQFSEKPNHSPGMSGGSYRPGSASPGGKPSGPGGPVGPLPTHFAQQVPPAQGSMVMHMRPPTTGPFPNPIQRPVMQVNKPVIIRSPPYPNPGRDPPHSIPPSASEPPVKGQEDGMKNKTIRDVRKAVGEGKTPSGGMTSKLQEPLPSTGQAKPARTGAIKPQAVKVEEGKA

Target ORF information:

RefSeq Version	XM_014413537.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014413537.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTTCCG 
CGACATGGCT TGCAGAGTCT TGGTAAAGTG GCCACAGCCC GGCGTATGCC CCCACCAGCT 
CATCTGCCGA GCTTGAAGTC TGAAAACAAA GGAAACGATC CCAACGTGAT TATAGTGCCC 
AAAGACGGTA CAGGATGGGC GAACAAGCAG GAACAACCCG ATCAAAAGAA TTCTGTTGCA 
TCAACACCAC AGCTGCCGGA GTTGCAGCCG CCGCTGCCTT TGCAGAAATC TGTCTCCAAT 
CTTCAGAAGT CCTCACCGGT AGCCAACCAG GAGAACACAA ACACAAGTGG ACCAAAGCAA 
TGGGCCCAGC TAAATGGAAA GGCAGTAGAG CAAGATGGTT CAAGGGCCTC AAACCGACTT 
CAGCCCTTCT CTCACGAGGA ATTTCCCACA CTGAAGGCAG CTGGAGAACA GGACAGGGCT 
GGCAAGGAAA GAAGCGGCTT CGATCCGTCG TATGGGCCCG GACCAAGCCT CCGCCCCCAG 
AATGTGACAA GCTGGAGGGA AGGTGGTGGC AGGAACCTTC AGCCCTCATC CCTGACCCTC 
AGCCTGCCAG CAGATCCTGA GGGTAAGGCC ACTGCCCTGG CTGAGACTGG CACCCCTCCA 
GCCTCGTCTC ACCCCTCCTC CGCCACAGTC ACCACCTCAT CTAATACGGT GACGGCTCCG 
TCACCAGGCC CTGACCCCAA GGAGCTTTCC CTGCGACCTG CTCAGCCTGT CCGCAGAACA 
GCCGTCCCTA CTGCATTGCA GTACCAGCTT CATCACACTT CAACTGCTGT CTACCATGAC 
ATGTTGCCTG CCTTTATGTG CTCTAAAGAG ACACGTGAAG CTACGGGTAC AGACCATGTT 
CCCACCACCG TAGCAGCCCC CGCCCGTTTT GACAGCAAAC CTACTTTTAG GCAGAGCTAT 
GCCAAACCTG AGCTTGTAAA TGGTGACGTA AGGAGAGAGA ATCGCTTTGT CCGTGCTGCG 
CCTCGACTTT CTTCACAGCC TATCCGTAGA CCTACCGACA GACCAGTTCG CCCAGCCATT 
ATTAATCCAG AGGATTTGAA GGATCTGGAT GAGCTTGACA ATGATTGTGA AGATGGATGG 
GCTGGACTTC ATGAGGAGGT TGATTATAGT GAGAAGCTCA AATTTAGTGA TGATGAAGAA 
GAACACTCTT CCAGTGACAA AAACAAGATC TGGGCTGACT GGGAGAGGGA GAGAGATAAC 
CAGCGGGACT GCCATTCCTC CCTTAGCTCG GGGGAGGCAT CTTACCCACA AGAGGGTCCA 
GAAGAGAGTT ACTCTTTCCA GCATCACCAC CACGAGCCTC CCAGGAAGAC CAACGGCAAA 
TATCTCTCCA CAGACACCCA GGTCCAACAG AGAAACCAAG GAGACCCACT TGCTGACCAG 
GATGATCACC AGCGGCAGTC TCAGGCACCA GCTAGGGCCA AGTATGTGTC GCCTGAGCTG 
TCTGAGGCTG TTGAGAGAGC CCGAAGACGG CGGGAGGAGG AAGAGAGGCG TGCTCGTGAG 
GAACGTCTGG CAGCATGTGC TGAAAAGCTC AAAAAACTGG ATGAAAAATT TGGGAAGACT 
GAAAGGCAGA CATCAAGGAC GGATGACAGC CAGAAGGAGG TAGAGGGAAA AGAAGTTCCA 
CTGTCCCCAA GTCGGGAACA GAGTCGAAGC CACCATGATA ACTGGCACTA CAGCACAAAA 
GATGGAAGTG AGTGTCCCTC GGACATTTCC CCTGGCCATA GTTACCGTGA AGAGTCTGGC 
TTCTCTACCT ACCGTGGCAG TGAAGACGAT GGCCCAGAGC CCTCCTCACC ATCAGGAGAC 
TACAGTGGGC GTCAGCCAAT CAAACCAGTG CCACCCCGCT TTCAAAAGCA ACCACAACAC 
CACCAGCAGC AGGAACAAAT GTTCAAGATG CAGCATTGGC AGCAGTCAGG TCACCCTAAT 
CCATCAGGGT CAAGCCACAC TCAGCGGGGC TACTACCCTC CACATGTCCT CGGTTTTGAT 
CCGCGCTGGA TGATGATGCC ACCTTTCATG GATCCCCGCA TGACCCAGGG ACGGTCTCCT 
GTTGACTACT ACCCTGCAGG AATGATAAAA CCTATGATGC ACCAAGATCA CTTGAACAGT 
CCCGGTTCTG ATGAGGGATG CCATCCAAAC TTGCCTCAGG AGAGACGAGC GCCTTCCACT 
GAGCCTTACC CAGTGTGGAA TCAAGATGGC TACCCCTTGC GTAGCTTTAC TCCACCTTAC 
CAGAGACAGC GTGAAAGCTC GGAAAGCGGA CATCCCGATG ACAGAGGTGA TATGGCCTCT 
TCCCAACAGG ACTCCTATGA AGAGAGGGCC AGTGATTGTT TGTCCCACAC TCAAGATGAT 
CTTCCCCATC ACAGTTATCA GAGCAGAGGT TCAGACAGAG AACAACATGA CCAAGGCTTG 
CTCACCACCG CTCAGAACCT CTCTCAGAAT TATTCAGATA GTGAATACCC AAAGCAAGAG 
TCGAGAGACA AGCATCTGAA AGATGGCCTT GAATCCCACG ACGAGGCCTT AGATGGCTCC 
ACAGACAACT GGAAAAGAGA TGGTAGCCAG AAACAAGACG GAGGGGTTAA CAGTGCCCAA 
AACCAGTGGT CTGAAGCCAG TTCCAGTTCA AGTAGTGTCA ACCAGCCATC TGAGGCTAGT 
GGACGCACCT TGATACGCAG AACTGGTCCC ATCAAGAAAC CAGTTCTTAA GGCTCTCAAA 
GTGGAAGACA AGGAGAATGA AAAGCCTAAA CCTGAGCCTG AGGAGAAGCC TGTCCCCTAC 
CGCCTGGAGA AAGAAGTCCT TACTAACGTT TATGACTTGA AGAAAGACAA CCAGCCTGCC 
AACAATAGGC GTTCTGCATC ACCTGTGGTT GAAAAACAAC CTGAAGAGAG GCAGCGCCAG 
TCTCCAGCTC CCAGTAAAAT GGACAGACCT CTGAGCACCC ACAGCGATGA CTCTCCCAAG 
GAGAACACTT GGAGCAGTGC TAAAAGCCAG TCACTTAGAG ACAGTCAGGA GAACCGAGAA 
CCCCAGGCAC CACGGCGCAA TAACTGGATC TTTATTGATG AAGAACAGGC CTTTGGTGCA 
GTGAGGGGGA CGGGTAGAGG CCGCAGTCGA GGCTTCCGGG AATTTAACTC CAGAGGTGGA 
ACCCGCGGTG GCCGCGGTGG AGATAATCTC AGAGGTGTTT ATAACAACAA TAACAACAGC 
AGTGGCAGCA GCACTCAGCG AGCAGGCAGA GGTCGAGCAC CACCCAGGGA TCTAATCAAG 
GTCGAGGAGT TTCAGAGGGG CAAACCCCGT AGGCGAAATG TCAGTGAAAC ATTGAGTGAA 
GCCTCAGAGT ATGAGGAACT TCCCAAGAGG CGTCGCCAGA AAGGATCTGA AAATGGAGAA 
GGCTATACAG AGTCTGGGGA TGTCCGTAAA GCTGATAGAG AGTCTTGGAG ATCCAACAAG 
GTGTACACAG ATGATCAGAC TGCCCCAGAT TCCAGAGAAA AGACCAAGGT CACCAGAGGC 
TTTGGAAATC GGGTGCTGCC TCCACGACTG AACACCACTG GTAGTTACAG TCGAAGCTTT 
GGAGGATCTA GAGATATTTC TACATGGAGG GGCCGTGGGC CTCAATTTAG TGGCAGCAGT 
AGCGGCTCGA TGCAAGAAAA TGGTTATGGT CCTGGAGCAG AGACTGCTTA CTCCCGCAGA 
CCCCCAGTTG AGCGTGACGC TCTCAAGTAC ACTCCTAAAT TTACTGGCTC CTTCATGGAA 
AATGGAACAG AGGAGCGTGA AGGAGAATAC TACTTTGACA GCGAGAACCC TGACAGACAG 
ATTTTAAGGA GAAGGCGTCC TCCACGTCAA GACAAGCCTC CGCGCTTCCG TCGTCTACGA 
CAAGAGCGTG AACCTGGCTC AAATCAGTGG ACAAGTGATG AGTTCATAAA CGGAGACTTT 
GCAAATCCCT GGCCAGGTCA TTCTAAAAGC ACTGGTGATG ATAACTGGCC CAGTGGCCAC 
TACTCTGGAC GTCCTAGCCA GCATGGCCAG ACAGAGGAAT GGGAGACGGG TTCAGACAAC 
AGCGACTTTG GTGACTGGAG AGAGAAACGG AGTGGAGGTG GAGGCATTGC TACACAGGGC 
CATGGCGATA TTCCCTCAGA CTCTGGCCAG GAGCCAGGCT CCAGTGAGAA GAGGGAGCTT 
TCTAAGAGAA GCTTCTCTAG TCAGAGACCA TTGGTGGAAC GGCAGAACAG GAAAGGAGAA 
CCATCACTGC TGGACGCAAG CAAGATGGTG CGTGCACCTG ATAATCCCCC CACCTCATCG 
AACAGGAGTG ACAGCTGGCA GAACGGAGGC ACTTCTTGTA AAAGCAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_023155191.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023010959.1 Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

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CloneID	OMa311653
Clone ID Related Accession (Same CDS sequence)	XM_023155191.1
Accession Version	XM_023155191.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein PRRC2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447383.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAACC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCACA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGAGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023010959.1
CDS	232..6900
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKNTVVPRHGLQSLGKVATARRMPPPAHLPSLKSENKGNDPNVIIVPKDGTGWANKQEQPDQKNSVASTPQLPELQPPLPLQKSVSNLQKSSPVANQENTNTSGPKQWAQLNGKAVEQDGSRASNRLQPFSHEEFPTLKAAGEQDRAGKERSGFDPSYGPGPSLRPQNVTSWREGGGRNLQPSSLTLSLPADPEGKATALAETGTPPASSHPSSATVTTSSNTVTAPSPGPDPKELSLRPAQPVRRTAVPTALQYQLHHTSTAVYHDMLPAFMCSKETREATGTDHVPTTVAAPARFDSKPTFRQSYAKPELVNGDVRRENRFVRAAPRLSSQPIRRPTDRPVRPAIINPEDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEEHSSSDKNKIWADWERERDNQRDCHSSLSSGEASYPQEGPEESYSFQHHHHEPPRKTNGKYLSTDTQVQQRNQGDPLADQDDHQRQSQAPARAKYVSPELSEAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTERQTSRTDDSQKEVEGKEVPLSPSREQSRSHHDNWHYSTKDGSECPSDISPGHSYREESGFSTYRGSEDDGPEPSSPSGDYSGRQPIKPVPPRFQKQPQHHQQQEQMFKMQHWQQSGHPNPSGSSHTQRGYYPPHVLGFDPRWMMMPPFMDPRMTQGRSPVDYYPAGMIKPMMHQDHLNSPGSDEGCHPNLPQERRAPSTEPYPVWNQDGYPLRSFTPPYQRQRESSESGHPDDRGDMASSQQDSYEERASDCLSHTQDDLPHHSYQSRGSDREQHDQGLLTTAQNLSQNYSDSEYPKQESRDKHLKDGLESHDEALDGSTDNWKRDGSQKQDGGVNSAQNQWSEASSSSSSVNQPSEASGRTLIRRTGPIKKPVLKALKVEDKENEKPKPEPEEKPVPYRLEKEVLTNVYDLKKDNQPANNRRSASPVVEKQPEERQRQSPAPSKMDRPLSTHSDDSPKENTWSSAKSQSLRDSQENREPQAPRRNNWIFIDEEQAFGAVRGTGRGRSRGFREFNSRGGTRGGRGGDNLRGVYNNNNNSSGSSTQRAGRGRAPPRDLIKVEEFQRGKPRRRNVSETLSEASEYEELPKRRRQKGSENGEGYTESGDVRKADRESWRSNKVYTDDQTAPDSREKTKVTRGFGNRVLPPRLNTTGSYSRSFGGSRDISTWRGRGPQFSGSSSGSMQENGYGPGAETAYSRRPPVERDALKYTPKFTGSFMENGTEEREGEYYFDSENPDRQILRRRRPPRQDKPPRFRRLRQEREPGSNQWTSDEFINGDFANPWPGHSKSTGDDNWPSGHYSGRPSQHGQTEEWETGSDNSDFGDWREKRSGGGGIATQGHGDIPSDSGQEPGSSEKRELSKRSFSSQRPLVERQNRKGEPSLLDASKMVRAPDNPPTSSNRSDSWQNGGTSCKRSPDESGSVYSIEQLPEEREPIEPAGKKLDKELKPGPVKTDITEPLSPYELSSYPIEGDAGGPGSNADGYQDALSKKQRRPQEDDRRRKEQGAAVPVKNRTVTSKMPPRFAKKQGSMSIEQPEEGISSNNLGTEIWETNSSALSVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQNKEQHKPGPIGNERSLKHRKGSEGVDRLEGGPITPVNGVDLHVDTVLPVPPIEFGVSAKDSDFSLPPGSTPVPVSNPVNKLQDTALNQSIPILRSNHLQPGINLNPISFPSADLTLKMESARKAWENSQSLPEQGSPGGSASGAQPPCSAGSSTGVSYSSFGGVSMPPMPVASVAPSMSMQGNHIPPLYLDGHVFPSQPRLVPPTMTQQQTYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEMFSSIPPFRSQVYMHPNLSQPSPMVLSGGAPLKGPYSAFPGMQPSDMVKPQSGSHYQPMNGSQQLVYDSQMNQGPGMGSSQLMDSQLIQVTMPLPGSQLRYGSAQQHLILPQSIQLQQGQNLSVGAPRRMLPPGSQAAVMTGSREGPQMEMKGFQFSEKPNHSPGMSGGSYRPGSASPGGKPSGPGGPVGPLPTHFAQQVPPAQGSMVMHMRPPTTGPFPNPIQRPVMQVNKPVIIRSPPYPNPGRDPPHSIPPSASEPPVKGQEDGMKNKTIRDVRKAVGEGKTPSGGMTSKLQEPLPSTGQAKPARTGAIKPQAVKVEEGKA

Target ORF information:

RefSeq Version	XM_023155191.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023155191.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAACC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCACA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGAGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_023155192.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023010960.1 Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

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CloneID	OMa311654
Clone ID Related Accession (Same CDS sequence)	XM_023155192.1
Accession Version	XM_023155192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein PRRC2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447383.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAACC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCACA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGAGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGCAG GAGTCCAGAT 
GAGTCAGGCT CTGTATACAG CATAGAGCAG CTGCCAGAAG AGAGGGAGCC CATTGAGCCC 
GCTGGGAAGA AATTAGACAA GGAGCTGAAG CCAGGACCTG TCAAAACAGA CATCACTGAA 
CCACTGTCCC CATATGAGCT CAGCAGCTAC CCAATTGAAG GAGACGCAGG GGGACCAGGT 
TCAAATGCAG ATGGATACCA GGATGCCTTG TCTAAAAAGC AAAGACGCCC ACAGGAAGAT 
GACAGGAGGA GGAAAGAACA GGGGGCAGCT GTACCAGTGA AAAACAGGAC AGTCACTTCC 
AAGATGCCAC CACGCTTTGC CAAAAAGCAG GGAAGCATGA GCATTGAACA ACCCGAGGAA 
GGAATTTCTT CTAACAATCT GGGAACTGAA ATCTGGGAGA CCAACAGCTC AGCTCTTTCA 
GTGCAGTCTT CAGGGGGAGA CTCATGGACC AAACAGGTGT CTTACACTGG GAGTGAGCCC 
AACTCTGAGG ACTCTGATGC GGGCCCAGAA CAAAACAAAG AGCAGCACAA ACCAGGGCCC 
ATCGGAAATG AACGCTCCCT GAAACACCGG AAGGGCTCAG AAGGTGTCGA TAGGCTGGAA 
GGTGGCCCTA TCACACCAGT CAATGGTGTG GACCTCCATG TGGACACTGT GCTGCCTGTC 
CCACCCATTG AGTTTGGTGT CAGTGCCAAA GATTCAGATT TCAGCCTGCC GCCGGGTTCT 
ACCCCAGTAC CCGTGTCCAA TCCTGTGAAC AAGCTTCAGG ACACTGCTTT GAATCAGAGT 
ATCCCCATTT TGCGCTCCAA CCACCTGCAG CCTGGCATCA ACCTCAACCC CATCTCCTTT 
CCCAGTGCTG ATCTCACTCT CAAGATGGAG TCAGCACGCA AGGCGTGGGA GAACTCCCAG 
TCTCTTCCTG AGCAGGGCTC TCCTGGTGGC AGCGCTTCAG GTGCTCAGCC TCCCTGCAGT 
GCCGGCTCAT CCACTGGTGT CAGTTACAGC TCGTTTGGAG GCGTTTCCAT GCCACCAATG 
CCCGTTGCAT CAGTAGCACC TTCCATGTCC ATGCAAGGGA ATCATATTCC CCCGCTGTAT 
CTGGATGGTC ACGTCTTTCC AAGCCAGCCA CGCCTTGTAC CACCTACCAT GACCCAGCAG 
CAGACTTACC AACAGGCGGC TGCAGCCCAG CAGATTCCCA TTTCTTTACA CACGTCTCTT 
CAGGCTCAGG CCCAGTTGGG GCTTCGGGGA GGTCTGCCTG TTTCTCAGTC CCAAGAGATG 
TTCAGCTCCA TCCCCCCCTT CAGGTCCCAG GTTTACATGC ACCCCAACTT GTCACAGCCC 
AGCCCCATGG TACTGTCAGG TGGAGCCCCT CTCAAGGGCC CATACTCAGC TTTCCCTGGC 
ATGCAGCCAT CAGACATGGT TAAACCCCAG TCAGGCTCAC ACTACCAGCC TATGAATGGC 
AGCCAGCAGC TAGTCTATGA TAGTCAGATG AACCAAGGGC CTGGCATGGG CTCTTCACAG 
TTAATGGACT CTCAGCTCAT TCAGGTCACT ATGCCCCTGC CTGGCTCTCA GCTGCGTTAT 
GGTTCAGCTC AACAACACCT CATCCTCCCA CAGTCCATCC AGCTGCAGCA GGGACAGAAC 
TTGTCAGTGG GAGCCCCACG CCGAATGCTA CCACCAGGGT CGCAGGCAGC TGTCATGACT 
GGCAGCAGAG AGGGTCCACA GATGGAAATG AAAGGTTTTC AGTTCTCTGA GAAGCCCAAT 
CATTCCCCAG GCATGTCTGG AGGTTCTTAC AGACCTGGCT CTGCTAGCCC CGGCGGGAAG 
CCCTCTGGTC CTGGAGGACC TGTAGGCCCT CTGCCTACAC ATTTTGCTCA GCAGGTCCCT 
CCTGCTCAGG GCAGCATGGT GATGCACATG CGTCCCCCCA CCACAGGCCC CTTCCCCAAC 
CCTATTCAGA GACCTGTTAT GCAGGTCAAT AAGCCCGTCA TCATCCGTTC CCCCCCTTAC 
CCCAATCCTG GCCGTGACCC TCCCCACTCC ATCCCTCCTT CAGCTTCCGA GCCCCCAGTT 
AAAGGGCAAG AGGATGGCAT GAAGGTGAGC CACCCACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023010960.1
CDS	232..6753
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKNTVVPRHGLQSLGKVATARRMPPPAHLPSLKSENKGNDPNVIIVPKDGTGWANKQEQPDQKNSVASTPQLPELQPPLPLQKSVSNLQKSSPVANQENTNTSGPKQWAQLNGKAVEQDGSRASNRLQPFSHEEFPTLKAAGEQDRAGKERSGFDPSYGPGPSLRPQNVTSWREGGGRNLQPSSLTLSLPADPEGKATALAETGTPPASSHPSSATVTTSSNTVTAPSPGPDPKELSLRPAQPVRRTAVPTALQYQLHHTSTAVYHDMLPAFMCSKETREATGTDHVPTTVAAPARFDSKPTFRQSYAKPELVNGDVRRENRFVRAAPRLSSQPIRRPTDRPVRPAIINPEDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEEHSSSDKNKIWADWERERDNQRDCHSSLSSGEASYPQEGPEESYSFQHHHHEPPRKTNGKYLSTDTQVQQRNQGDPLADQDDHQRQSQAPARAKYVSPELSEAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTERQTSRTDDSQKEVEGKEVPLSPSREQSRSHHDNWHYSTKDGSECPSDISPGHSYREESGFSTYRGSEDDGPEPSSPSGDYSGRQPIKPVPPRFQKQPQHHQQQEQMFKMQHWQQSGHPNPSGSSHTQRGYYPPHVLGFDPRWMMMPPFMDPRMTQGRSPVDYYPAGMIKPMMHQDHLNSPGSDEGCHPNLPQERRAPSTEPYPVWNQDGYPLRSFTPPYQRQRESSESGHPDDRGDMASSQQDSYEERASDCLSHTQDDLPHHSYQSRGSDREQHDQGLLTTAQNLSQNYSDSEYPKQESRDKHLKDGLESHDEALDGSTDNWKRDGSQKQDGGVNSAQNQWSEASSSSSSVNQPSEASGRTLIRRTGPIKKPVLKALKVEDKENEKPKPEPEEKPVPYRLEKEVLTNVYDLKKDNQPANNRRSASPVVEKQPEERQRQSPAPSKMDRPLSTHSDDSPKENTWSSAKSQSLRDSQENREPQAPRRNNWIFIDEEQAFGAVRGTGRGRSRGFREFNSRGGTRGGRGGDNLRGVYNNNNNSSGSSTQRAGRGRAPPRDLIKVEEFQRGKPRRRNVSETLSEASEYEELPKRRRQKGSENGEGYTESGDVRKADRESWRSNKVYTDDQTAPDSREKTKVTRGFGNRVLPPRLNTTGSYSRSFGGSRDISTWRGRGPQFSGSSSGSMQENGYGPGAETAYSRRPPVERDALKYTPKFTGSFMENGTEEREGEYYFDSENPDRQILRRRRPPRQDKPPRFRRLRQEREPGSNQWTSDEFINGDFANPWPGHSKSTGDDNWPSGHYSGRPSQHGQTEEWETGSDNSDFGDWREKRSGGGGIATQGHGDIPSDSGQEPGSSEKRELSKRSFSSQRPLVERQNRKGEPSLLDASKMVRAPDNPPTSSNRSDSWQNGGTSCKSRSPDESGSVYSIEQLPEEREPIEPAGKKLDKELKPGPVKTDITEPLSPYELSSYPIEGDAGGPGSNADGYQDALSKKQRRPQEDDRRRKEQGAAVPVKNRTVTSKMPPRFAKKQGSMSIEQPEEGISSNNLGTEIWETNSSALSVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQNKEQHKPGPIGNERSLKHRKGSEGVDRLEGGPITPVNGVDLHVDTVLPVPPIEFGVSAKDSDFSLPPGSTPVPVSNPVNKLQDTALNQSIPILRSNHLQPGINLNPISFPSADLTLKMESARKAWENSQSLPEQGSPGGSASGAQPPCSAGSSTGVSYSSFGGVSMPPMPVASVAPSMSMQGNHIPPLYLDGHVFPSQPRLVPPTMTQQQTYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEMFSSIPPFRSQVYMHPNLSQPSPMVLSGGAPLKGPYSAFPGMQPSDMVKPQSGSHYQPMNGSQQLVYDSQMNQGPGMGSSQLMDSQLIQVTMPLPGSQLRYGSAQQHLILPQSIQLQQGQNLSVGAPRRMLPPGSQAAVMTGSREGPQMEMKGFQFSEKPNHSPGMSGGSYRPGSASPGGKPSGPGGPVGPLPTHFAQQVPPAQGSMVMHMRPPTTGPFPNPIQRPVMQVNKPVIIRSPPYPNPGRDPPHSIPPSASEPPVKGQEDGMKVSHPL

Target ORF information:

RefSeq Version	XM_023155192.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023155192.1

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAACC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCACA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGAGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGCAG GAGTCCAGAT 
GAGTCAGGCT CTGTATACAG CATAGAGCAG CTGCCAGAAG AGAGGGAGCC CATTGAGCCC 
GCTGGGAAGA AATTAGACAA GGAGCTGAAG CCAGGACCTG TCAAAACAGA CATCACTGAA 
CCACTGTCCC CATATGAGCT CAGCAGCTAC CCAATTGAAG GAGACGCAGG GGGACCAGGT 
TCAAATGCAG ATGGATACCA GGATGCCTTG TCTAAAAAGC AAAGACGCCC ACAGGAAGAT 
GACAGGAGGA GGAAAGAACA GGGGGCAGCT GTACCAGTGA AAAACAGGAC AGTCACTTCC 
AAGATGCCAC CACGCTTTGC CAAAAAGCAG GGAAGCATGA GCATTGAACA ACCCGAGGAA 
GGAATTTCTT CTAACAATCT GGGAACTGAA ATCTGGGAGA CCAACAGCTC AGCTCTTTCA 
GTGCAGTCTT CAGGGGGAGA CTCATGGACC AAACAGGTGT CTTACACTGG GAGTGAGCCC 
AACTCTGAGG ACTCTGATGC GGGCCCAGAA CAAAACAAAG AGCAGCACAA ACCAGGGCCC 
ATCGGAAATG AACGCTCCCT GAAACACCGG AAGGGCTCAG AAGGTGTCGA TAGGCTGGAA 
GGTGGCCCTA TCACACCAGT CAATGGTGTG GACCTCCATG TGGACACTGT GCTGCCTGTC 
CCACCCATTG AGTTTGGTGT CAGTGCCAAA GATTCAGATT TCAGCCTGCC GCCGGGTTCT 
ACCCCAGTAC CCGTGTCCAA TCCTGTGAAC AAGCTTCAGG ACACTGCTTT GAATCAGAGT 
ATCCCCATTT TGCGCTCCAA CCACCTGCAG CCTGGCATCA ACCTCAACCC CATCTCCTTT 
CCCAGTGCTG ATCTCACTCT CAAGATGGAG TCAGCACGCA AGGCGTGGGA GAACTCCCAG 
TCTCTTCCTG AGCAGGGCTC TCCTGGTGGC AGCGCTTCAG GTGCTCAGCC TCCCTGCAGT 
GCCGGCTCAT CCACTGGTGT CAGTTACAGC TCGTTTGGAG GCGTTTCCAT GCCACCAATG 
CCCGTTGCAT CAGTAGCACC TTCCATGTCC ATGCAAGGGA ATCATATTCC CCCGCTGTAT 
CTGGATGGTC ACGTCTTTCC AAGCCAGCCA CGCCTTGTAC CACCTACCAT GACCCAGCAG 
CAGACTTACC AACAGGCGGC TGCAGCCCAG CAGATTCCCA TTTCTTTACA CACGTCTCTT 
CAGGCTCAGG CCCAGTTGGG GCTTCGGGGA GGTCTGCCTG TTTCTCAGTC CCAAGAGATG 
TTCAGCTCCA TCCCCCCCTT CAGGTCCCAG GTTTACATGC ACCCCAACTT GTCACAGCCC 
AGCCCCATGG TACTGTCAGG TGGAGCCCCT CTCAAGGGCC CATACTCAGC TTTCCCTGGC 
ATGCAGCCAT CAGACATGGT TAAACCCCAG TCAGGCTCAC ACTACCAGCC TATGAATGGC 
AGCCAGCAGC TAGTCTATGA TAGTCAGATG AACCAAGGGC CTGGCATGGG CTCTTCACAG 
TTAATGGACT CTCAGCTCAT TCAGGTCACT ATGCCCCTGC CTGGCTCTCA GCTGCGTTAT 
GGTTCAGCTC AACAACACCT CATCCTCCCA CAGTCCATCC AGCTGCAGCA GGGACAGAAC 
TTGTCAGTGG GAGCCCCACG CCGAATGCTA CCACCAGGGT CGCAGGCAGC TGTCATGACT 
GGCAGCAGAG AGGGTCCACA GATGGAAATG AAAGGTTTTC AGTTCTCTGA GAAGCCCAAT 
CATTCCCCAG GCATGTCTGG AGGTTCTTAC AGACCTGGCT CTGCTAGCCC CGGCGGGAAG 
CCCTCTGGTC CTGGAGGACC TGTAGGCCCT CTGCCTACAC ATTTTGCTCA GCAGGTCCCT 
CCTGCTCAGG GCAGCATGGT GATGCACATG CGTCCCCCCA CCACAGGCCC CTTCCCCAAC 
CCTATTCAGA GACCTGTTAT GCAGGTCAAT AAGCCCGTCA TCATCCGTTC CCCCCCTTAC 
CCCAATCCTG GCCGTGACCC TCCCCACTCC ATCCCTCCTT CAGCTTCCGA GCCCCCAGTT 
AAAGGGCAAG AGGATGGCAT GAAGGTGAGC CACCCACTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_014413536.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014269022.1 Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

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CloneID	OMa374705
Clone ID Related Accession (Same CDS sequence)	XM_014413536.2
Accession Version	XM_014413536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein PRRC2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014413536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAATC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCGCA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGGGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGCAG GAGTCCAGAT 
GAGTCAGGCT CTGTATACAG CATAGAGCAG CTGCCAGAAG AGAGGGAGCC CATTGAGCCC 
GCTGGGAAGA AATTAGACAA GGAGCTGAAG CCAGGACCTG TCAAAACAGA CATCACTGAA 
CCACTGTCCC CATATGAGCT CAGCAGCTAC CCAATTGAAG GAGACGCAGG GGGACCAGGT 
TCAAATGCAG ATGGATACCA GGATGCCTTG TCTAAAAAGC AAAGACGCCC ACAGGAAGAT 
GACAGGAGGA GGAAAGAACA GGGGGCAGCT GTACCAGTGA AAAACAGGAC AGTCACTTCC 
AAGATGCCAC CACGCTTTGC CAAAAAGCAG GGAAGCATGA GCATTGAACA ACCCGAGGAA 
GGAATTTCTT CTAACAATCT GGGAACTGAA ATCTGGGAGA CCAACAGCTC AGCTCTTTCA 
GTGCAGTCTT CAGGGGGAGA CTCATGGACC AAACAGGTGT CTTACACTGG GAGTGAGCCC 
AACTCTGAGG ACTCTGATGC GGGCCCAGAA CAAAACAAAG AGCAGCACAA ACCAGGGCCC 
ATCGGAAATG AACGCTCCCT GAAACACCGG AAGGGCTCAG AAGGTGTCGA TAGGCTGGAA 
GGTGGCCCTA TCACACCAGT CAATGGTGTG GACCTCCATG TGGACACTGT GCTGCCTGTC 
CCACCCATTG AGTTTGGTGT CAGTGCCAAA GATTCAGATT TCAGCCTGCC GCCGGGTTCT 
ACCCCAGTAC CCGTGTCCAA TCCTGTGAAC AAGCTTCAGG ACACTGCTTT GAATCAGAGT 
ATCCCCATTT TGCGCTCCAA CCACCTGCAG CCTGGCATCA ACCTCAACCC CATCTCCTTT 
CCCAGTGCTG ATCTCACTCT CAAGATGGAG TCAGCACGCA AGGCGTGGGA GAACTCCCAG 
TCTCTTCCTG AGCAGGGCTC TCCTGGTGGC AGCGCTTCAG GTGCTCAGCC TCCCTGCAGT 
GCCGGCTCAT CCACTGGTGT CAGTTACAGC TCGTTTGGAG GCGTTTCCAT GCCACCAATG 
CCCGTTGCAT CAGTAGCACC TTCCATGTCC ATGCAAGGGA ATCATATTCC CCCGCTGTAT 
CTGGATGGTC ACGTCTTTCC AAGCCAGCCA CGCCTTGTAC CACCTACCAT GACCCAGCAG 
CAGACTTACC AACAGGCGGC TGCAGCCCAG CAGATTCCCA TTTCTTTACA CACGTCTCTT 
CAGGCTCAGG CCCAGTTGGG GCTTCGGGGA GGTCTGCCTG TTTCTCAGTC CCAAGAGATG 
TTCAGCTCCA TCCCCCCCTT CAGGTCCCAG GTTTACATGC ACCCCAACTT GTCACAGCCC 
AGCCCCATGG TACTGTCAGG TGGAGCCCCT CTCAAGGGCC CATACTCAGC TTTCCCTGGC 
ATGCAGCCAT CAGACATGGT TAAACCCCAG TCAGGCTCAC ACTACCAGCC TATGAATGGC 
AGCCAGCAGC TAGTCTATGA TAGTCAGATG AACCAAGGGC CTGGCATGGG CTCTTCACAG 
TTAATGGACT CTCAGCTCAT TCAGGTCACT ATGCCCCTGC CTGGCTCTCA GCTGCGTTAT 
GGTTCAGCTC AACAACACCT CATCCTCCCA CAGTCCATCC AGCTGCAGCA GGGACAGAAC 
TTGTCAGTGG GAGCCCCACG CCGAATGCTA CCACCAGGGT CGCAGGCAGC TGTCATGACT 
GGCAGCAGAG AGGGTCCACA GATGGAAATG AAAGGTTTTC AGTTCTCTGA GAAGCCCAAT 
CATTCCCCAG GCATGTCTGG AGGTTCTTAC AGACCTGGCT CTGCTAGCCC CGGCGGGAAG 
CCCTCTGGTC CTGGAGGACC TGTAGGCCCT CTGCCTACAC ATTTTGCTCA GCAGGTCCCT 
CCTGCTCAGG GCAGCATGGT GATGCACATG CGTCCCCCCA CCACAGGCCC CTTCCCCAAC 
CCTATTCAGA GACCTGTTAT GCAGGTCAAT AAGCCCGTCA TCATCCGTTC CCCCCCTTAC 
CCCAATCCTG GCCGTGACCC TCCCCACTCC ATCCCTCCTT CAGCTTCCGA GCCCCCAGTT 
AAAGGGCAAG AGGATGGCAT GAAGAATAAA ACAATAAGAG ATGTACGCAA GGCGGTGGGA 
GAGGGCAAAA CACCGTCCGG GGGCATGACC AGCAAACTCC AGGAGCCCCT ACCTTCCACA 
GGCCAAGCCA AACCAGCACG CACTGGAGCC ATCAAACCCC AGGCTGTCAA AGTAGAAGAG 
GGCAAGGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014269022.1
CDS	232..6903
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKNTVVPRHGLQSLGKVATARRMPPPAHLPSLKSENKGNDPNVIIVPKDGTGWANKQEQPDQKNSVASTPQLPELQPPLPLQKSVSNLQKSSPVANQENTNTSGPKQWAQLNGKAVEQDGSRASNRLQPFSHEEFPTLKAAGEQDRAGKERSGFDPSYGPGPSLRPQNVTSWREGGGRNLQPSSLTLSLPADPEGKATALAETGTPPASSHPSSATVTTSSNTVTAPSPGPDPKELSLRPAQPVRRTAVPTALQYQLHHTSTAVYHDMLPAFMCSKETREATGTDHVPTTVAAPARFDSKPTFRQSYAKPELVNGDVRRENRFVRAAPRLSSQPIRRPTDRPVRPAIINPEDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEEHSSSDKNKIWADWERERDNQRDCHSSLSSGEASYPQEGPEESYSFQHHHHEPPRKTNGKYLSTDTQVQQRNQGDPLADQDDHQRQSQAPARAKYVSPELSEAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTERQTSRTDDSQKEVEGKEVPLSPSREQSRSHHDNWHYSTKDGSECPSDISPGHSYREESGFSTYRGSEDDGPEPSSPSGDYSGRQPIKPVPPRFQKQPQHHQQQEQMFKMQHWQQSGHPNPSGSSHTQRGYYPPHVLGFDPRWMMMPPFMDPRMTQGRSPVDYYPAGMIKPMMHQDHLNSPGSDEGCHPNLPQERRAPSTEPYPVWNQDGYPLRSFTPPYQRQRESSESGHPDDRGDMASSQQDSYEERASDCLSHTQDDLPHHSYQSRGSDREQHDQGLLTTAQNLSQNYSDSEYPKQESRDKHLKDGLESHDEALDGSTDNWKRDGSQKQDGGVNSAQNQWSEASSSSSSVNQPSEASGRTLIRRTGPIKKPVLKALKVEDKENEKPKPEPEEKPVPYRLEKEVLTNVYDLKKDNQPANNRRSASPVVEKQPEERQRQSPAPSKMDRPLSTHSDDSPKENTWSSAKSQSLRDSQENREPQAPRRNNWIFIDEEQAFGAVRGTGRGRSRGFREFNSRGGTRGGRGGDNLRGVYNNNNNSSGSSTQRAGRGRAPPRDLIKVEEFQRGKPRRRNVSETLSEASEYEELPKRRRQKGSENGEGYTESGDVRKADRESWRSNKVYTDDQTAPDSREKTKVTRGFGNRVLPPRLNTTGSYSRSFGGSRDISTWRGRGPQFSGSSSGSMQENGYGPGAETAYSRRPPVERDALKYTPKFTGSFMENGTEEREGEYYFDSENPDRQILRRRRPPRQDKPPRFRRLRQEREPGSNQWTSDEFINGDFANPWPGHSKSTGDDNWPSGHYSGRPSQHGQTEEWETGSDNSDFGDWREKRSGGGGIATQGHGDIPSDSGQEPGSSEKRELSKRSFSSQRPLVERQNRKGEPSLLDASKMVRAPDNPPTSSNRSDSWQNGGTSCKSRSPDESGSVYSIEQLPEEREPIEPAGKKLDKELKPGPVKTDITEPLSPYELSSYPIEGDAGGPGSNADGYQDALSKKQRRPQEDDRRRKEQGAAVPVKNRTVTSKMPPRFAKKQGSMSIEQPEEGISSNNLGTEIWETNSSALSVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQNKEQHKPGPIGNERSLKHRKGSEGVDRLEGGPITPVNGVDLHVDTVLPVPPIEFGVSAKDSDFSLPPGSTPVPVSNPVNKLQDTALNQSIPILRSNHLQPGINLNPISFPSADLTLKMESARKAWENSQSLPEQGSPGGSASGAQPPCSAGSSTGVSYSSFGGVSMPPMPVASVAPSMSMQGNHIPPLYLDGHVFPSQPRLVPPTMTQQQTYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEMFSSIPPFRSQVYMHPNLSQPSPMVLSGGAPLKGPYSAFPGMQPSDMVKPQSGSHYQPMNGSQQLVYDSQMNQGPGMGSSQLMDSQLIQVTMPLPGSQLRYGSAQQHLILPQSIQLQQGQNLSVGAPRRMLPPGSQAAVMTGSREGPQMEMKGFQFSEKPNHSPGMSGGSYRPGSASPGGKPSGPGGPVGPLPTHFAQQVPPAQGSMVMHMRPPTTGPFPNPIQRPVMQVNKPVIIRSPPYPNPGRDPPHSIPPSASEPPVKGQEDGMKNKTIRDVRKAVGEGKTPSGGMTSKLQEPLPSTGQAKPARTGAIKPQAVKVEEGKA

Target ORF information:

RefSeq Version	XM_014413536.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014413536.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAATC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCGCA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGGGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGCAG GAGTCCAGAT 
GAGTCAGGCT CTGTATACAG CATAGAGCAG CTGCCAGAAG AGAGGGAGCC CATTGAGCCC 
GCTGGGAAGA AATTAGACAA GGAGCTGAAG CCAGGACCTG TCAAAACAGA CATCACTGAA 
CCACTGTCCC CATATGAGCT CAGCAGCTAC CCAATTGAAG GAGACGCAGG GGGACCAGGT 
TCAAATGCAG ATGGATACCA GGATGCCTTG TCTAAAAAGC AAAGACGCCC ACAGGAAGAT 
GACAGGAGGA GGAAAGAACA GGGGGCAGCT GTACCAGTGA AAAACAGGAC AGTCACTTCC 
AAGATGCCAC CACGCTTTGC CAAAAAGCAG GGAAGCATGA GCATTGAACA ACCCGAGGAA 
GGAATTTCTT CTAACAATCT GGGAACTGAA ATCTGGGAGA CCAACAGCTC AGCTCTTTCA 
GTGCAGTCTT CAGGGGGAGA CTCATGGACC AAACAGGTGT CTTACACTGG GAGTGAGCCC 
AACTCTGAGG ACTCTGATGC GGGCCCAGAA CAAAACAAAG AGCAGCACAA ACCAGGGCCC 
ATCGGAAATG AACGCTCCCT GAAACACCGG AAGGGCTCAG AAGGTGTCGA TAGGCTGGAA 
GGTGGCCCTA TCACACCAGT CAATGGTGTG GACCTCCATG TGGACACTGT GCTGCCTGTC 
CCACCCATTG AGTTTGGTGT CAGTGCCAAA GATTCAGATT TCAGCCTGCC GCCGGGTTCT 
ACCCCAGTAC CCGTGTCCAA TCCTGTGAAC AAGCTTCAGG ACACTGCTTT GAATCAGAGT 
ATCCCCATTT TGCGCTCCAA CCACCTGCAG CCTGGCATCA ACCTCAACCC CATCTCCTTT 
CCCAGTGCTG ATCTCACTCT CAAGATGGAG TCAGCACGCA AGGCGTGGGA GAACTCCCAG 
TCTCTTCCTG AGCAGGGCTC TCCTGGTGGC AGCGCTTCAG GTGCTCAGCC TCCCTGCAGT 
GCCGGCTCAT CCACTGGTGT CAGTTACAGC TCGTTTGGAG GCGTTTCCAT GCCACCAATG 
CCCGTTGCAT CAGTAGCACC TTCCATGTCC ATGCAAGGGA ATCATATTCC CCCGCTGTAT 
CTGGATGGTC ACGTCTTTCC AAGCCAGCCA CGCCTTGTAC CACCTACCAT GACCCAGCAG 
CAGACTTACC AACAGGCGGC TGCAGCCCAG CAGATTCCCA TTTCTTTACA CACGTCTCTT 
CAGGCTCAGG CCCAGTTGGG GCTTCGGGGA GGTCTGCCTG TTTCTCAGTC CCAAGAGATG 
TTCAGCTCCA TCCCCCCCTT CAGGTCCCAG GTTTACATGC ACCCCAACTT GTCACAGCCC 
AGCCCCATGG TACTGTCAGG TGGAGCCCCT CTCAAGGGCC CATACTCAGC TTTCCCTGGC 
ATGCAGCCAT CAGACATGGT TAAACCCCAG TCAGGCTCAC ACTACCAGCC TATGAATGGC 
AGCCAGCAGC TAGTCTATGA TAGTCAGATG AACCAAGGGC CTGGCATGGG CTCTTCACAG 
TTAATGGACT CTCAGCTCAT TCAGGTCACT ATGCCCCTGC CTGGCTCTCA GCTGCGTTAT 
GGTTCAGCTC AACAACACCT CATCCTCCCA CAGTCCATCC AGCTGCAGCA GGGACAGAAC 
TTGTCAGTGG GAGCCCCACG CCGAATGCTA CCACCAGGGT CGCAGGCAGC TGTCATGACT 
GGCAGCAGAG AGGGTCCACA GATGGAAATG AAAGGTTTTC AGTTCTCTGA GAAGCCCAAT 
CATTCCCCAG GCATGTCTGG AGGTTCTTAC AGACCTGGCT CTGCTAGCCC CGGCGGGAAG 
CCCTCTGGTC CTGGAGGACC TGTAGGCCCT CTGCCTACAC ATTTTGCTCA GCAGGTCCCT 
CCTGCTCAGG GCAGCATGGT GATGCACATG CGTCCCCCCA CCACAGGCCC CTTCCCCAAC 
CCTATTCAGA GACCTGTTAT GCAGGTCAAT AAGCCCGTCA TCATCCGTTC CCCCCCTTAC 
CCCAATCCTG GCCGTGACCC TCCCCACTCC ATCCCTCCTT CAGCTTCCGA GCCCCCAGTT 
AAAGGGCAAG AGGATGGCAT GAAGAATAAA ACAATAAGAG ATGTACGCAA GGCGGTGGGA 
GAGGGCAAAA CACCGTCCGG GGGCATGACC AGCAAACTCC AGGAGCCCCT ACCTTCCACA 
GGCCAAGCCA AACCAGCACG CACTGGAGCC ATCAAACCCC AGGCTGTCAA AGTAGAAGAG 
GGCAAGGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_014413537.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014269023.1 Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

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CloneID	OMa374706
Clone ID Related Accession (Same CDS sequence)	XM_014413537.2
Accession Version	XM_014413537.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein PRRC2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_014413537.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTTCCG 
CGACATGGCT TGCAGAGTCT TGGTAAAGTG GCCACAGCCC GGCGTATGCC CCCACCAGCT 
CATCTGCCGA GCTTGAAGTC TGAAAACAAA GGAAACGATC CCAACGTGAT TATAGTGCCC 
AAAGACGGTA CAGGATGGGC GAACAAGCAG GAACAACCCG ATCAAAAGAA TTCTGTTGCA 
TCAACACCAC AGCTGCCGGA GTTGCAGCCG CCGCTGCCTT TGCAGAAATC TGTCTCCAAT 
CTTCAGAAGT CCTCACCGGT AGCCAACCAG GAGAACACAA ACACAAGTGG ACCAAAGCAA 
TGGGCCCAGC TAAATGGAAA GGCAGTAGAG CAAGATGGTT CAAGGGCCTC AAACCGACTT 
CAGCCCTTCT CTCACGAGGA ATTTCCCACA CTGAAGGCAG CTGGAGAACA GGACAGGGCT 
GGCAAGGAAA GAAGCGGCTT CGATCCGTCG TATGGGCCCG GACCAAGCCT CCGCCCCCAG 
AATGTGACAA GCTGGAGGGA AGGTGGTGGC AGGAATCTTC AGCCCTCATC CCTGACCCTC 
AGCCTGCCAG CAGATCCTGA GGGTAAGGCC ACTGCCCTGG CTGAGACTGG CACCCCTCCA 
GCCTCGTCTC ACCCCTCCTC CGCCACAGTC ACCACCTCAT CTAATACGGT GACGGCTCCG 
TCACCAGGCC CTGACCCCAA GGAGCTTTCC CTGCGACCTG CTCAGCCTGT CCGCAGAACA 
GCCGTCCCTA CTGCATTGCA GTACCAGCTT CATCACACTT CAACTGCTGT CTACCATGAC 
ATGTTGCCTG CCTTTATGTG CTCTAAAGAG ACACGTGAAG CTACGGGTAC AGACCATGTT 
CCCACCACCG TAGCAGCCCC CGCCCGTTTT GACAGCAAAC CTACTTTTAG GCAGAGCTAT 
GCCAAACCTG AGCTTGTAAA TGGTGACGTA AGGAGAGAGA ATCGCTTTGT CCGTGCTGCG 
CCTCGACTTT CTTCGCAGCC TATCCGTAGA CCTACCGACA GACCAGTTCG CCCAGCCATT 
ATTAATCCAG AGGATTTGAA GGATCTGGAT GAGCTTGACA ATGATTGTGA AGATGGATGG 
GCTGGACTTC ATGAGGAGGT TGATTATAGT GAGAAGCTCA AATTTAGTGA TGATGAAGAA 
GAACACTCTT CCAGTGACAA AAACAAGATC TGGGCTGACT GGGAGAGGGA GAGAGATAAC 
CAGCGGGACT GCCATTCCTC CCTTAGCTCG GGGGAGGCAT CTTACCCACA AGAGGGTCCA 
GAAGAGAGTT ACTCTTTCCA GCATCACCAC CACGAGCCTC CCAGGAAGAC CAACGGCAAA 
TATCTCTCCA CAGACACCCA GGTCCAACAG AGAAACCAAG GAGACCCACT TGCTGACCAG 
GATGATCACC AGCGGCAGTC TCAGGCACCA GCTAGGGCCA AGTATGTGTC GCCTGAGCTG 
TCTGAGGCTG TTGAGAGAGC CCGAAGACGG CGGGAGGAGG AAGAGAGGCG TGCTCGTGAG 
GAACGTCTGG CAGCATGTGC TGAAAAGCTC AAAAAACTGG ATGAAAAATT TGGGAAGACT 
GAAAGGCAGA CATCAAGGAC GGATGACAGC CAGAAGGAGG TAGAGGGAAA AGAAGTTCCA 
CTGTCCCCAA GTCGGGAACA GAGTCGAAGC CACCATGATA ACTGGCACTA CAGCACAAAA 
GATGGAAGTG AGTGTCCCTC GGACATTTCC CCTGGCCATA GTTACCGTGA AGAGTCTGGC 
TTCTCTACCT ACCGTGGCAG TGAAGACGAT GGCCCAGAGC CCTCCTCACC ATCAGGAGAC 
TACAGTGGGC GTCAGCCAAT CAAACCAGTG CCACCCCGCT TTCAAAAGCA ACCACAACAC 
CACCAGCAGC AGGAACAAAT GTTCAAGATG CAGCATTGGC AGCAGTCAGG TCACCCTAAT 
CCATCAGGGT CAAGCCACAC TCAGCGGGGC TACTACCCTC CACATGTCCT CGGTTTTGAT 
CCGCGCTGGA TGATGATGCC ACCTTTCATG GATCCCCGCA TGACCCAGGG ACGGTCTCCT 
GTTGACTACT ACCCTGCAGG AATGATAAAA CCTATGATGC ACCAAGATCA CTTGAACAGT 
CCCGGTTCTG ATGAGGGATG CCATCCAAAC TTGCCTCAGG AGAGACGAGC GCCTTCCACT 
GAGCCTTACC CAGTGTGGAA TCAAGATGGC TACCCCTTGC GTAGCTTTAC TCCACCTTAC 
CAGAGACAGC GTGAAAGCTC GGAAAGCGGA CATCCCGATG ACAGAGGTGA TATGGCCTCT 
TCCCAACAGG ACTCCTATGA AGAGAGGGCC AGTGATTGTT TGTCCCACAC TCAAGATGAT 
CTTCCCCATC ACAGTTATCA GAGCAGAGGT TCAGACAGAG AACAACATGA CCAAGGCTTG 
CTCACCACCG CTCAGAACCT CTCTCAGAAT TATTCAGATA GTGAATACCC AAAGCAAGAG 
TCGAGAGACA AGCATCTGAA AGATGGCCTT GAATCCCACG ACGAGGCCTT AGATGGCTCC 
ACAGACAACT GGAAAAGAGA TGGTAGCCAG AAACAAGACG GAGGGGTTAA CAGTGCCCAA 
AACCAGTGGT CTGAAGCCAG TTCCAGTTCA AGTAGTGTCA ACCAGCCATC TGAGGCTAGT 
GGACGCACCT TGATACGCAG AACTGGTCCC ATCAAGAAAC CAGTTCTTAA GGCTCTCAAA 
GTGGAAGACA AGGAGAATGA AAAGCCTAAA CCTGAGCCTG AGGAGAAGCC TGTCCCCTAC 
CGCCTGGAGA AAGAAGTCCT TACTAACGTT TATGACTTGA AGAAAGACAA CCAGCCTGCC 
AACAATAGGC GTTCTGCATC ACCTGTGGTT GAAAAACAAC CTGAAGAGAG GCAGCGCCAG 
TCTCCAGCTC CCAGTAAAAT GGACAGACCT CTGAGCACCC ACAGCGATGA CTCTCCCAAG 
GAGAACACTT GGAGCAGTGC TAAAAGCCAG TCACTTAGAG ACAGTCAGGA GAACCGAGAA 
CCCCAGGCAC CACGGCGCAA TAACTGGATC TTTATTGATG AAGAACAGGC CTTTGGTGCA 
GTGAGGGGGA CGGGTAGAGG CCGCAGTCGA GGCTTCCGGG AATTTAACTC CAGAGGTGGA 
ACCCGCGGTG GCCGCGGTGG AGATAATCTC AGAGGTGTTT ATAACAACAA TAACAACAGC 
AGTGGCAGCA GCACTCAGCG AGCAGGCAGA GGTCGAGCAC CACCCAGGGA TCTAATCAAG 
GTCGAGGAGT TTCAGAGGGG CAAACCCCGT AGGCGAAATG TCAGTGAAAC ATTGAGTGAA 
GCCTCAGAGT ATGAGGAACT TCCCAAGAGG CGTCGCCAGA AAGGATCTGA AAATGGAGAA 
GGCTATACAG AGTCTGGGGA TGTCCGTAAA GCTGATAGAG AGTCTTGGAG ATCCAACAAG 
GTGTACACAG ATGATCAGAC TGCCCCAGAT TCCAGAGAAA AGACCAAGGT CACCAGAGGC 
TTTGGAAATC GGGTGCTGCC TCCACGACTG AACACCACTG GTAGTTACAG TCGAAGCTTT 
GGAGGATCTA GAGATATTTC TACATGGAGG GGCCGTGGGC CTCAATTTAG TGGCAGCAGT 
AGCGGCTCGA TGCAAGAAAA TGGTTATGGT CCTGGAGCAG AGACTGCTTA CTCCCGCAGA 
CCCCCAGTTG AGCGTGACGC TCTCAAGTAC ACTCCTAAAT TTACTGGCTC CTTCATGGAA 
AATGGAACAG AGGAGCGTGA AGGAGAATAC TACTTTGACA GCGAGAACCC TGACAGACAG 
ATTTTAAGGA GAAGGCGTCC TCCACGTCAA GACAAGCCTC CGCGCTTCCG TCGTCTACGA 
CAAGAGCGTG AACCTGGCTC AAATCAGTGG ACAAGTGATG AGTTCATAAA CGGGGACTTT 
GCAAATCCCT GGCCAGGTCA TTCTAAAAGC ACTGGTGATG ATAACTGGCC CAGTGGCCAC 
TACTCTGGAC GTCCTAGCCA GCATGGCCAG ACAGAGGAAT GGGAGACGGG TTCAGACAAC 
AGCGACTTTG GTGACTGGAG AGAGAAACGG AGTGGAGGTG GAGGCATTGC TACACAGGGC 
CATGGCGATA TTCCCTCAGA CTCTGGCCAG GAGCCAGGCT CCAGTGAGAA GAGGGAGCTT 
TCTAAGAGAA GCTTCTCTAG TCAGAGACCA TTGGTGGAAC GGCAGAACAG GAAAGGAGAA 
CCATCACTGC TGGACGCAAG CAAGATGGTG CGTGCACCTG ATAATCCCCC CACCTCATCG 
AACAGGAGTG ACAGCTGGCA GAACGGAGGC ACTTCTTGTA AAAGCAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014269023.1
CDS	232..6900
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKNTVPRHGLQSLGKVATARRMPPPAHLPSLKSENKGNDPNVIIVPKDGTGWANKQEQPDQKNSVASTPQLPELQPPLPLQKSVSNLQKSSPVANQENTNTSGPKQWAQLNGKAVEQDGSRASNRLQPFSHEEFPTLKAAGEQDRAGKERSGFDPSYGPGPSLRPQNVTSWREGGGRNLQPSSLTLSLPADPEGKATALAETGTPPASSHPSSATVTTSSNTVTAPSPGPDPKELSLRPAQPVRRTAVPTALQYQLHHTSTAVYHDMLPAFMCSKETREATGTDHVPTTVAAPARFDSKPTFRQSYAKPELVNGDVRRENRFVRAAPRLSSQPIRRPTDRPVRPAIINPEDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEEHSSSDKNKIWADWERERDNQRDCHSSLSSGEASYPQEGPEESYSFQHHHHEPPRKTNGKYLSTDTQVQQRNQGDPLADQDDHQRQSQAPARAKYVSPELSEAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTERQTSRTDDSQKEVEGKEVPLSPSREQSRSHHDNWHYSTKDGSECPSDISPGHSYREESGFSTYRGSEDDGPEPSSPSGDYSGRQPIKPVPPRFQKQPQHHQQQEQMFKMQHWQQSGHPNPSGSSHTQRGYYPPHVLGFDPRWMMMPPFMDPRMTQGRSPVDYYPAGMIKPMMHQDHLNSPGSDEGCHPNLPQERRAPSTEPYPVWNQDGYPLRSFTPPYQRQRESSESGHPDDRGDMASSQQDSYEERASDCLSHTQDDLPHHSYQSRGSDREQHDQGLLTTAQNLSQNYSDSEYPKQESRDKHLKDGLESHDEALDGSTDNWKRDGSQKQDGGVNSAQNQWSEASSSSSSVNQPSEASGRTLIRRTGPIKKPVLKALKVEDKENEKPKPEPEEKPVPYRLEKEVLTNVYDLKKDNQPANNRRSASPVVEKQPEERQRQSPAPSKMDRPLSTHSDDSPKENTWSSAKSQSLRDSQENREPQAPRRNNWIFIDEEQAFGAVRGTGRGRSRGFREFNSRGGTRGGRGGDNLRGVYNNNNNSSGSSTQRAGRGRAPPRDLIKVEEFQRGKPRRRNVSETLSEASEYEELPKRRRQKGSENGEGYTESGDVRKADRESWRSNKVYTDDQTAPDSREKTKVTRGFGNRVLPPRLNTTGSYSRSFGGSRDISTWRGRGPQFSGSSSGSMQENGYGPGAETAYSRRPPVERDALKYTPKFTGSFMENGTEEREGEYYFDSENPDRQILRRRRPPRQDKPPRFRRLRQEREPGSNQWTSDEFINGDFANPWPGHSKSTGDDNWPSGHYSGRPSQHGQTEEWETGSDNSDFGDWREKRSGGGGIATQGHGDIPSDSGQEPGSSEKRELSKRSFSSQRPLVERQNRKGEPSLLDASKMVRAPDNPPTSSNRSDSWQNGGTSCKSRSPDESGSVYSIEQLPEEREPIEPAGKKLDKELKPGPVKTDITEPLSPYELSSYPIEGDAGGPGSNADGYQDALSKKQRRPQEDDRRRKEQGAAVPVKNRTVTSKMPPRFAKKQGSMSIEQPEEGISSNNLGTEIWETNSSALSVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQNKEQHKPGPIGNERSLKHRKGSEGVDRLEGGPITPVNGVDLHVDTVLPVPPIEFGVSAKDSDFSLPPGSTPVPVSNPVNKLQDTALNQSIPILRSNHLQPGINLNPISFPSADLTLKMESARKAWENSQSLPEQGSPGGSASGAQPPCSAGSSTGVSYSSFGGVSMPPMPVASVAPSMSMQGNHIPPLYLDGHVFPSQPRLVPPTMTQQQTYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEMFSSIPPFRSQVYMHPNLSQPSPMVLSGGAPLKGPYSAFPGMQPSDMVKPQSGSHYQPMNGSQQLVYDSQMNQGPGMGSSQLMDSQLIQVTMPLPGSQLRYGSAQQHLILPQSIQLQQGQNLSVGAPRRMLPPGSQAAVMTGSREGPQMEMKGFQFSEKPNHSPGMSGGSYRPGSASPGGKPSGPGGPVGPLPTHFAQQVPPAQGSMVMHMRPPTTGPFPNPIQRPVMQVNKPVIIRSPPYPNPGRDPPHSIPPSASEPPVKGQEDGMKNKTIRDVRKAVGEGKTPSGGMTSKLQEPLPSTGQAKPARTGAIKPQAVKVEEGKA

Target ORF information:

RefSeq Version	XM_014413537.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014413537.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTTCCG 
CGACATGGCT TGCAGAGTCT TGGTAAAGTG GCCACAGCCC GGCGTATGCC CCCACCAGCT 
CATCTGCCGA GCTTGAAGTC TGAAAACAAA GGAAACGATC CCAACGTGAT TATAGTGCCC 
AAAGACGGTA CAGGATGGGC GAACAAGCAG GAACAACCCG ATCAAAAGAA TTCTGTTGCA 
TCAACACCAC AGCTGCCGGA GTTGCAGCCG CCGCTGCCTT TGCAGAAATC TGTCTCCAAT 
CTTCAGAAGT CCTCACCGGT AGCCAACCAG GAGAACACAA ACACAAGTGG ACCAAAGCAA 
TGGGCCCAGC TAAATGGAAA GGCAGTAGAG CAAGATGGTT CAAGGGCCTC AAACCGACTT 
CAGCCCTTCT CTCACGAGGA ATTTCCCACA CTGAAGGCAG CTGGAGAACA GGACAGGGCT 
GGCAAGGAAA GAAGCGGCTT CGATCCGTCG TATGGGCCCG GACCAAGCCT CCGCCCCCAG 
AATGTGACAA GCTGGAGGGA AGGTGGTGGC AGGAATCTTC AGCCCTCATC CCTGACCCTC 
AGCCTGCCAG CAGATCCTGA GGGTAAGGCC ACTGCCCTGG CTGAGACTGG CACCCCTCCA 
GCCTCGTCTC ACCCCTCCTC CGCCACAGTC ACCACCTCAT CTAATACGGT GACGGCTCCG 
TCACCAGGCC CTGACCCCAA GGAGCTTTCC CTGCGACCTG CTCAGCCTGT CCGCAGAACA 
GCCGTCCCTA CTGCATTGCA GTACCAGCTT CATCACACTT CAACTGCTGT CTACCATGAC 
ATGTTGCCTG CCTTTATGTG CTCTAAAGAG ACACGTGAAG CTACGGGTAC AGACCATGTT 
CCCACCACCG TAGCAGCCCC CGCCCGTTTT GACAGCAAAC CTACTTTTAG GCAGAGCTAT 
GCCAAACCTG AGCTTGTAAA TGGTGACGTA AGGAGAGAGA ATCGCTTTGT CCGTGCTGCG 
CCTCGACTTT CTTCGCAGCC TATCCGTAGA CCTACCGACA GACCAGTTCG CCCAGCCATT 
ATTAATCCAG AGGATTTGAA GGATCTGGAT GAGCTTGACA ATGATTGTGA AGATGGATGG 
GCTGGACTTC ATGAGGAGGT TGATTATAGT GAGAAGCTCA AATTTAGTGA TGATGAAGAA 
GAACACTCTT CCAGTGACAA AAACAAGATC TGGGCTGACT GGGAGAGGGA GAGAGATAAC 
CAGCGGGACT GCCATTCCTC CCTTAGCTCG GGGGAGGCAT CTTACCCACA AGAGGGTCCA 
GAAGAGAGTT ACTCTTTCCA GCATCACCAC CACGAGCCTC CCAGGAAGAC CAACGGCAAA 
TATCTCTCCA CAGACACCCA GGTCCAACAG AGAAACCAAG GAGACCCACT TGCTGACCAG 
GATGATCACC AGCGGCAGTC TCAGGCACCA GCTAGGGCCA AGTATGTGTC GCCTGAGCTG 
TCTGAGGCTG TTGAGAGAGC CCGAAGACGG CGGGAGGAGG AAGAGAGGCG TGCTCGTGAG 
GAACGTCTGG CAGCATGTGC TGAAAAGCTC AAAAAACTGG ATGAAAAATT TGGGAAGACT 
GAAAGGCAGA CATCAAGGAC GGATGACAGC CAGAAGGAGG TAGAGGGAAA AGAAGTTCCA 
CTGTCCCCAA GTCGGGAACA GAGTCGAAGC CACCATGATA ACTGGCACTA CAGCACAAAA 
GATGGAAGTG AGTGTCCCTC GGACATTTCC CCTGGCCATA GTTACCGTGA AGAGTCTGGC 
TTCTCTACCT ACCGTGGCAG TGAAGACGAT GGCCCAGAGC CCTCCTCACC ATCAGGAGAC 
TACAGTGGGC GTCAGCCAAT CAAACCAGTG CCACCCCGCT TTCAAAAGCA ACCACAACAC 
CACCAGCAGC AGGAACAAAT GTTCAAGATG CAGCATTGGC AGCAGTCAGG TCACCCTAAT 
CCATCAGGGT CAAGCCACAC TCAGCGGGGC TACTACCCTC CACATGTCCT CGGTTTTGAT 
CCGCGCTGGA TGATGATGCC ACCTTTCATG GATCCCCGCA TGACCCAGGG ACGGTCTCCT 
GTTGACTACT ACCCTGCAGG AATGATAAAA CCTATGATGC ACCAAGATCA CTTGAACAGT 
CCCGGTTCTG ATGAGGGATG CCATCCAAAC TTGCCTCAGG AGAGACGAGC GCCTTCCACT 
GAGCCTTACC CAGTGTGGAA TCAAGATGGC TACCCCTTGC GTAGCTTTAC TCCACCTTAC 
CAGAGACAGC GTGAAAGCTC GGAAAGCGGA CATCCCGATG ACAGAGGTGA TATGGCCTCT 
TCCCAACAGG ACTCCTATGA AGAGAGGGCC AGTGATTGTT TGTCCCACAC TCAAGATGAT 
CTTCCCCATC ACAGTTATCA GAGCAGAGGT TCAGACAGAG AACAACATGA CCAAGGCTTG 
CTCACCACCG CTCAGAACCT CTCTCAGAAT TATTCAGATA GTGAATACCC AAAGCAAGAG 
TCGAGAGACA AGCATCTGAA AGATGGCCTT GAATCCCACG ACGAGGCCTT AGATGGCTCC 
ACAGACAACT GGAAAAGAGA TGGTAGCCAG AAACAAGACG GAGGGGTTAA CAGTGCCCAA 
AACCAGTGGT CTGAAGCCAG TTCCAGTTCA AGTAGTGTCA ACCAGCCATC TGAGGCTAGT 
GGACGCACCT TGATACGCAG AACTGGTCCC ATCAAGAAAC CAGTTCTTAA GGCTCTCAAA 
GTGGAAGACA AGGAGAATGA AAAGCCTAAA CCTGAGCCTG AGGAGAAGCC TGTCCCCTAC 
CGCCTGGAGA AAGAAGTCCT TACTAACGTT TATGACTTGA AGAAAGACAA CCAGCCTGCC 
AACAATAGGC GTTCTGCATC ACCTGTGGTT GAAAAACAAC CTGAAGAGAG GCAGCGCCAG 
TCTCCAGCTC CCAGTAAAAT GGACAGACCT CTGAGCACCC ACAGCGATGA CTCTCCCAAG 
GAGAACACTT GGAGCAGTGC TAAAAGCCAG TCACTTAGAG ACAGTCAGGA GAACCGAGAA 
CCCCAGGCAC CACGGCGCAA TAACTGGATC TTTATTGATG AAGAACAGGC CTTTGGTGCA 
GTGAGGGGGA CGGGTAGAGG CCGCAGTCGA GGCTTCCGGG AATTTAACTC CAGAGGTGGA 
ACCCGCGGTG GCCGCGGTGG AGATAATCTC AGAGGTGTTT ATAACAACAA TAACAACAGC 
AGTGGCAGCA GCACTCAGCG AGCAGGCAGA GGTCGAGCAC CACCCAGGGA TCTAATCAAG 
GTCGAGGAGT TTCAGAGGGG CAAACCCCGT AGGCGAAATG TCAGTGAAAC ATTGAGTGAA 
GCCTCAGAGT ATGAGGAACT TCCCAAGAGG CGTCGCCAGA AAGGATCTGA AAATGGAGAA 
GGCTATACAG AGTCTGGGGA TGTCCGTAAA GCTGATAGAG AGTCTTGGAG ATCCAACAAG 
GTGTACACAG ATGATCAGAC TGCCCCAGAT TCCAGAGAAA AGACCAAGGT CACCAGAGGC 
TTTGGAAATC GGGTGCTGCC TCCACGACTG AACACCACTG GTAGTTACAG TCGAAGCTTT 
GGAGGATCTA GAGATATTTC TACATGGAGG GGCCGTGGGC CTCAATTTAG TGGCAGCAGT 
AGCGGCTCGA TGCAAGAAAA TGGTTATGGT CCTGGAGCAG AGACTGCTTA CTCCCGCAGA 
CCCCCAGTTG AGCGTGACGC TCTCAAGTAC ACTCCTAAAT TTACTGGCTC CTTCATGGAA 
AATGGAACAG AGGAGCGTGA AGGAGAATAC TACTTTGACA GCGAGAACCC TGACAGACAG 
ATTTTAAGGA GAAGGCGTCC TCCACGTCAA GACAAGCCTC CGCGCTTCCG TCGTCTACGA 
CAAGAGCGTG AACCTGGCTC AAATCAGTGG ACAAGTGATG AGTTCATAAA CGGGGACTTT 
GCAAATCCCT GGCCAGGTCA TTCTAAAAGC ACTGGTGATG ATAACTGGCC CAGTGGCCAC 
TACTCTGGAC GTCCTAGCCA GCATGGCCAG ACAGAGGAAT GGGAGACGGG TTCAGACAAC 
AGCGACTTTG GTGACTGGAG AGAGAAACGG AGTGGAGGTG GAGGCATTGC TACACAGGGC 
CATGGCGATA TTCCCTCAGA CTCTGGCCAG GAGCCAGGCT CCAGTGAGAA GAGGGAGCTT 
TCTAAGAGAA GCTTCTCTAG TCAGAGACCA TTGGTGGAAC GGCAGAACAG GAAAGGAGAA 
CCATCACTGC TGGACGCAAG CAAGATGGTG CGTGCACCTG ATAATCCCCC CACCTCATCG 
AACAGGAGTG ACAGCTGGCA GAACGGAGGC ACTTCTTGTA AAAGCAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prrc2b ( XM_023155191.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_023010959.1 Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

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CloneID	OMa374707
Clone ID Related Accession (Same CDS sequence)	XM_023155191.2
Accession Version	XM_023155191.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	protein PRRC2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_023155191.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAATC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCGCA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGGGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023010959.1
CDS	232..6900
Translation	MSDRLGQITKSKDGKSKYSSLSLFDKYKGKSIETQKNTVVPRHGLQSLGKVATARRMPPPAHLPSLKSENKGNDPNVIIVPKDGTGWANKQEQPDQKNSVASTPQLPELQPPLPLQKSVSNLQKSSPVANQENTNTSGPKQWAQLNGKAVEQDGSRASNRLQPFSHEEFPTLKAAGEQDRAGKERSGFDPSYGPGPSLRPQNVTSWREGGGRNLQPSSLTLSLPADPEGKATALAETGTPPASSHPSSATVTTSSNTVTAPSPGPDPKELSLRPAQPVRRTAVPTALQYQLHHTSTAVYHDMLPAFMCSKETREATGTDHVPTTVAAPARFDSKPTFRQSYAKPELVNGDVRRENRFVRAAPRLSSQPIRRPTDRPVRPAIINPEDLKDLDELDNDCEDGWAGLHEEVDYSEKLKFSDDEEEHSSSDKNKIWADWERERDNQRDCHSSLSSGEASYPQEGPEESYSFQHHHHEPPRKTNGKYLSTDTQVQQRNQGDPLADQDDHQRQSQAPARAKYVSPELSEAVERARRRREEEERRAREERLAACAEKLKKLDEKFGKTERQTSRTDDSQKEVEGKEVPLSPSREQSRSHHDNWHYSTKDGSECPSDISPGHSYREESGFSTYRGSEDDGPEPSSPSGDYSGRQPIKPVPPRFQKQPQHHQQQEQMFKMQHWQQSGHPNPSGSSHTQRGYYPPHVLGFDPRWMMMPPFMDPRMTQGRSPVDYYPAGMIKPMMHQDHLNSPGSDEGCHPNLPQERRAPSTEPYPVWNQDGYPLRSFTPPYQRQRESSESGHPDDRGDMASSQQDSYEERASDCLSHTQDDLPHHSYQSRGSDREQHDQGLLTTAQNLSQNYSDSEYPKQESRDKHLKDGLESHDEALDGSTDNWKRDGSQKQDGGVNSAQNQWSEASSSSSSVNQPSEASGRTLIRRTGPIKKPVLKALKVEDKENEKPKPEPEEKPVPYRLEKEVLTNVYDLKKDNQPANNRRSASPVVEKQPEERQRQSPAPSKMDRPLSTHSDDSPKENTWSSAKSQSLRDSQENREPQAPRRNNWIFIDEEQAFGAVRGTGRGRSRGFREFNSRGGTRGGRGGDNLRGVYNNNNNSSGSSTQRAGRGRAPPRDLIKVEEFQRGKPRRRNVSETLSEASEYEELPKRRRQKGSENGEGYTESGDVRKADRESWRSNKVYTDDQTAPDSREKTKVTRGFGNRVLPPRLNTTGSYSRSFGGSRDISTWRGRGPQFSGSSSGSMQENGYGPGAETAYSRRPPVERDALKYTPKFTGSFMENGTEEREGEYYFDSENPDRQILRRRRPPRQDKPPRFRRLRQEREPGSNQWTSDEFINGDFANPWPGHSKSTGDDNWPSGHYSGRPSQHGQTEEWETGSDNSDFGDWREKRSGGGGIATQGHGDIPSDSGQEPGSSEKRELSKRSFSSQRPLVERQNRKGEPSLLDASKMVRAPDNPPTSSNRSDSWQNGGTSCKRSPDESGSVYSIEQLPEEREPIEPAGKKLDKELKPGPVKTDITEPLSPYELSSYPIEGDAGGPGSNADGYQDALSKKQRRPQEDDRRRKEQGAAVPVKNRTVTSKMPPRFAKKQGSMSIEQPEEGISSNNLGTEIWETNSSALSVQSSGGDSWTKQVSYTGSEPNSEDSDAGPEQNKEQHKPGPIGNERSLKHRKGSEGVDRLEGGPITPVNGVDLHVDTVLPVPPIEFGVSAKDSDFSLPPGSTPVPVSNPVNKLQDTALNQSIPILRSNHLQPGINLNPISFPSADLTLKMESARKAWENSQSLPEQGSPGGSASGAQPPCSAGSSTGVSYSSFGGVSMPPMPVASVAPSMSMQGNHIPPLYLDGHVFPSQPRLVPPTMTQQQTYQQAAAAQQIPISLHTSLQAQAQLGLRGGLPVSQSQEMFSSIPPFRSQVYMHPNLSQPSPMVLSGGAPLKGPYSAFPGMQPSDMVKPQSGSHYQPMNGSQQLVYDSQMNQGPGMGSSQLMDSQLIQVTMPLPGSQLRYGSAQQHLILPQSIQLQQGQNLSVGAPRRMLPPGSQAAVMTGSREGPQMEMKGFQFSEKPNHSPGMSGGSYRPGSASPGGKPSGPGGPVGPLPTHFAQQVPPAQGSMVMHMRPPTTGPFPNPIQRPVMQVNKPVIIRSPPYPNPGRDPPHSIPPSASEPPVKGQEDGMKNKTIRDVRKAVGEGKTPSGGMTSKLQEPLPSTGQAKPARTGAIKPQAVKVEEGKA

Target ORF information:

RefSeq Version	XM_023155191.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra proline rich coiled-coil 2B (prrc2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023155191.2

ORF Insert Sequence:

ATGTCCGATC GTTTGGGGCA AATAACCAAG TCCAAGGATG GAAAAAGCAA GTATTCCTCA 
CTCAGCCTAT TTGATAAGTA CAAGGGAAAA TCAATAGAAA CTCAGAAAAA CACAGTAGTT 
CCGCGACATG GCTTGCAGAG TCTTGGTAAA GTGGCCACAG CCCGGCGTAT GCCCCCACCA 
GCTCATCTGC CGAGCTTGAA GTCTGAAAAC AAAGGAAACG ATCCCAACGT GATTATAGTG 
CCCAAAGACG GTACAGGATG GGCGAACAAG CAGGAACAAC CCGATCAAAA GAATTCTGTT 
GCATCAACAC CACAGCTGCC GGAGTTGCAG CCGCCGCTGC CTTTGCAGAA ATCTGTCTCC 
AATCTTCAGA AGTCCTCACC GGTAGCCAAC CAGGAGAACA CAAACACAAG TGGACCAAAG 
CAATGGGCCC AGCTAAATGG AAAGGCAGTA GAGCAAGATG GTTCAAGGGC CTCAAACCGA 
CTTCAGCCCT TCTCTCACGA GGAATTTCCC ACACTGAAGG CAGCTGGAGA ACAGGACAGG 
GCTGGCAAGG AAAGAAGCGG CTTCGATCCG TCGTATGGGC CCGGACCAAG CCTCCGCCCC 
CAGAATGTGA CAAGCTGGAG GGAAGGTGGT GGCAGGAATC TTCAGCCCTC ATCCCTGACC 
CTCAGCCTGC CAGCAGATCC TGAGGGTAAG GCCACTGCCC TGGCTGAGAC TGGCACCCCT 
CCAGCCTCGT CTCACCCCTC CTCCGCCACA GTCACCACCT CATCTAATAC GGTGACGGCT 
CCGTCACCAG GCCCTGACCC CAAGGAGCTT TCCCTGCGAC CTGCTCAGCC TGTCCGCAGA 
ACAGCCGTCC CTACTGCATT GCAGTACCAG CTTCATCACA CTTCAACTGC TGTCTACCAT 
GACATGTTGC CTGCCTTTAT GTGCTCTAAA GAGACACGTG AAGCTACGGG TACAGACCAT 
GTTCCCACCA CCGTAGCAGC CCCCGCCCGT TTTGACAGCA AACCTACTTT TAGGCAGAGC 
TATGCCAAAC CTGAGCTTGT AAATGGTGAC GTAAGGAGAG AGAATCGCTT TGTCCGTGCT 
GCGCCTCGAC TTTCTTCGCA GCCTATCCGT AGACCTACCG ACAGACCAGT TCGCCCAGCC 
ATTATTAATC CAGAGGATTT GAAGGATCTG GATGAGCTTG ACAATGATTG TGAAGATGGA 
TGGGCTGGAC TTCATGAGGA GGTTGATTAT AGTGAGAAGC TCAAATTTAG TGATGATGAA 
GAAGAACACT CTTCCAGTGA CAAAAACAAG ATCTGGGCTG ACTGGGAGAG GGAGAGAGAT 
AACCAGCGGG ACTGCCATTC CTCCCTTAGC TCGGGGGAGG CATCTTACCC ACAAGAGGGT 
CCAGAAGAGA GTTACTCTTT CCAGCATCAC CACCACGAGC CTCCCAGGAA GACCAACGGC 
AAATATCTCT CCACAGACAC CCAGGTCCAA CAGAGAAACC AAGGAGACCC ACTTGCTGAC 
CAGGATGATC ACCAGCGGCA GTCTCAGGCA CCAGCTAGGG CCAAGTATGT GTCGCCTGAG 
CTGTCTGAGG CTGTTGAGAG AGCCCGAAGA CGGCGGGAGG AGGAAGAGAG GCGTGCTCGT 
GAGGAACGTC TGGCAGCATG TGCTGAAAAG CTCAAAAAAC TGGATGAAAA ATTTGGGAAG 
ACTGAAAGGC AGACATCAAG GACGGATGAC AGCCAGAAGG AGGTAGAGGG AAAAGAAGTT 
CCACTGTCCC CAAGTCGGGA ACAGAGTCGA AGCCACCATG ATAACTGGCA CTACAGCACA 
AAAGATGGAA GTGAGTGTCC CTCGGACATT TCCCCTGGCC ATAGTTACCG TGAAGAGTCT 
GGCTTCTCTA CCTACCGTGG CAGTGAAGAC GATGGCCCAG AGCCCTCCTC ACCATCAGGA 
GACTACAGTG GGCGTCAGCC AATCAAACCA GTGCCACCCC GCTTTCAAAA GCAACCACAA 
CACCACCAGC AGCAGGAACA AATGTTCAAG ATGCAGCATT GGCAGCAGTC AGGTCACCCT 
AATCCATCAG GGTCAAGCCA CACTCAGCGG GGCTACTACC CTCCACATGT CCTCGGTTTT 
GATCCGCGCT GGATGATGAT GCCACCTTTC ATGGATCCCC GCATGACCCA GGGACGGTCT 
CCTGTTGACT ACTACCCTGC AGGAATGATA AAACCTATGA TGCACCAAGA TCACTTGAAC 
AGTCCCGGTT CTGATGAGGG ATGCCATCCA AACTTGCCTC AGGAGAGACG AGCGCCTTCC 
ACTGAGCCTT ACCCAGTGTG GAATCAAGAT GGCTACCCCT TGCGTAGCTT TACTCCACCT 
TACCAGAGAC AGCGTGAAAG CTCGGAAAGC GGACATCCCG ATGACAGAGG TGATATGGCC 
TCTTCCCAAC AGGACTCCTA TGAAGAGAGG GCCAGTGATT GTTTGTCCCA CACTCAAGAT 
GATCTTCCCC ATCACAGTTA TCAGAGCAGA GGTTCAGACA GAGAACAACA TGACCAAGGC 
TTGCTCACCA CCGCTCAGAA CCTCTCTCAG AATTATTCAG ATAGTGAATA CCCAAAGCAA 
GAGTCGAGAG ACAAGCATCT GAAAGATGGC CTTGAATCCC ACGACGAGGC CTTAGATGGC 
TCCACAGACA ACTGGAAAAG AGATGGTAGC CAGAAACAAG ACGGAGGGGT TAACAGTGCC 
CAAAACCAGT GGTCTGAAGC CAGTTCCAGT TCAAGTAGTG TCAACCAGCC ATCTGAGGCT 
AGTGGACGCA CCTTGATACG CAGAACTGGT CCCATCAAGA AACCAGTTCT TAAGGCTCTC 
AAAGTGGAAG ACAAGGAGAA TGAAAAGCCT AAACCTGAGC CTGAGGAGAA GCCTGTCCCC 
TACCGCCTGG AGAAAGAAGT CCTTACTAAC GTTTATGACT TGAAGAAAGA CAACCAGCCT 
GCCAACAATA GGCGTTCTGC ATCACCTGTG GTTGAAAAAC AACCTGAAGA GAGGCAGCGC 
CAGTCTCCAG CTCCCAGTAA AATGGACAGA CCTCTGAGCA CCCACAGCGA TGACTCTCCC 
AAGGAGAACA CTTGGAGCAG TGCTAAAAGC CAGTCACTTA GAGACAGTCA GGAGAACCGA 
GAACCCCAGG CACCACGGCG CAATAACTGG ATCTTTATTG ATGAAGAACA GGCCTTTGGT 
GCAGTGAGGG GGACGGGTAG AGGCCGCAGT CGAGGCTTCC GGGAATTTAA CTCCAGAGGT 
GGAACCCGCG GTGGCCGCGG TGGAGATAAT CTCAGAGGTG TTTATAACAA CAATAACAAC 
AGCAGTGGCA GCAGCACTCA GCGAGCAGGC AGAGGTCGAG CACCACCCAG GGATCTAATC 
AAGGTCGAGG AGTTTCAGAG GGGCAAACCC CGTAGGCGAA ATGTCAGTGA AACATTGAGT 
GAAGCCTCAG AGTATGAGGA ACTTCCCAAG AGGCGTCGCC AGAAAGGATC TGAAAATGGA 
GAAGGCTATA CAGAGTCTGG GGATGTCCGT AAAGCTGATA GAGAGTCTTG GAGATCCAAC 
AAGGTGTACA CAGATGATCA GACTGCCCCA GATTCCAGAG AAAAGACCAA GGTCACCAGA 
GGCTTTGGAA ATCGGGTGCT GCCTCCACGA CTGAACACCA CTGGTAGTTA CAGTCGAAGC 
TTTGGAGGAT CTAGAGATAT TTCTACATGG AGGGGCCGTG GGCCTCAATT TAGTGGCAGC 
AGTAGCGGCT CGATGCAAGA AAATGGTTAT GGTCCTGGAG CAGAGACTGC TTACTCCCGC 
AGACCCCCAG TTGAGCGTGA CGCTCTCAAG TACACTCCTA AATTTACTGG CTCCTTCATG 
GAAAATGGAA CAGAGGAGCG TGAAGGAGAA TACTACTTTG ACAGCGAGAA CCCTGACAGA 
CAGATTTTAA GGAGAAGGCG TCCTCCACGT CAAGACAAGC CTCCGCGCTT CCGTCGTCTA 
CGACAAGAGC GTGAACCTGG CTCAAATCAG TGGACAAGTG ATGAGTTCAT AAACGGGGAC 
TTTGCAAATC CCTGGCCAGG TCATTCTAAA AGCACTGGTG ATGATAACTG GCCCAGTGGC 
CACTACTCTG GACGTCCTAG CCAGCATGGC CAGACAGAGG AATGGGAGAC GGGTTCAGAC 
AACAGCGACT TTGGTGACTG GAGAGAGAAA CGGAGTGGAG GTGGAGGCAT TGCTACACAG 
GGCCATGGCG ATATTCCCTC AGACTCTGGC CAGGAGCCAG GCTCCAGTGA GAAGAGGGAG 
CTTTCTAAGA GAAGCTTCTC TAGTCAGAGA CCATTGGTGG AACGGCAGAA CAGGAAAGGA 
GAACCATCAC TGCTGGACGC AAGCAAGATG GTGCGTGCAC CTGATAATCC CCCCACCTCA 
TCGAACAGGA GTGACAGCTG GCAGAACGGA GGCACTTCTT GTAAAAGGAG TCCAGATGAG 
TCAGGCTCTG TATACAGCAT AGAGCAGCTG CCAGAAGAGA GGGAGCCCAT TGAGCCCGCT 
GGGAAGAAAT TAGACAAGGA GCTGAAGCCA GGACCTGTCA AAACAGACAT CACTGAACCA 
CTGTCCCCAT ATGAGCTCAG CAGCTACCCA ATTGAAGGAG ACGCAGGGGG ACCAGGTTCA 
AATGCAGATG GATACCAGGA TGCCTTGTCT AAAAAGCAAA GACGCCCACA GGAAGATGAC 
AGGAGGAGGA AAGAACAGGG GGCAGCTGTA CCAGTGAAAA ACAGGACAGT CACTTCCAAG 
ATGCCACCAC GCTTTGCCAA AAAGCAGGGA AGCATGAGCA TTGAACAACC CGAGGAAGGA 
ATTTCTTCTA ACAATCTGGG AACTGAAATC TGGGAGACCA ACAGCTCAGC TCTTTCAGTG 
CAGTCTTCAG GGGGAGACTC ATGGACCAAA CAGGTGTCTT ACACTGGGAG TGAGCCCAAC 
TCTGAGGACT CTGATGCGGG CCCAGAACAA AACAAAGAGC AGCACAAACC AGGGCCCATC 
GGAAATGAAC GCTCCCTGAA ACACCGGAAG GGCTCAGAAG GTGTCGATAG GCTGGAAGGT 
GGCCCTATCA CACCAGTCAA TGGTGTGGAC CTCCATGTGG ACACTGTGCT GCCTGTCCCA 
CCCATTGAGT TTGGTGTCAG TGCCAAAGAT TCAGATTTCA GCCTGCCGCC GGGTTCTACC 
CCAGTACCCG TGTCCAATCC TGTGAACAAG CTTCAGGACA CTGCTTTGAA TCAGAGTATC 
CCCATTTTGC GCTCCAACCA CCTGCAGCCT GGCATCAACC TCAACCCCAT CTCCTTTCCC 
AGTGCTGATC TCACTCTCAA GATGGAGTCA GCACGCAAGG CGTGGGAGAA CTCCCAGTCT 
CTTCCTGAGC AGGGCTCTCC TGGTGGCAGC GCTTCAGGTG CTCAGCCTCC CTGCAGTGCC 
GGCTCATCCA CTGGTGTCAG TTACAGCTCG TTTGGAGGCG TTTCCATGCC ACCAATGCCC 
GTTGCATCAG TAGCACCTTC CATGTCCATG CAAGGGAATC ATATTCCCCC GCTGTATCTG 
GATGGTCACG TCTTTCCAAG CCAGCCACGC CTTGTACCAC CTACCATGAC CCAGCAGCAG 
ACTTACCAAC AGGCGGCTGC AGCCCAGCAG ATTCCCATTT CTTTACACAC GTCTCTTCAG 
GCTCAGGCCC AGTTGGGGCT TCGGGGAGGT CTGCCTGTTT CTCAGTCCCA AGAGATGTTC 
AGCTCCATCC CCCCCTTCAG GTCCCAGGTT TACATGCACC CCAACTTGTC ACAGCCCAGC 
CCCATGGTAC TGTCAGGTGG AGCCCCTCTC AAGGGCCCAT ACTCAGCTTT CCCTGGCATG 
CAGCCATCAG ACATGGTTAA ACCCCAGTCA GGCTCACACT ACCAGCCTAT GAATGGCAGC 
CAGCAGCTAG TCTATGATAG TCAGATGAAC CAAGGGCCTG GCATGGGCTC TTCACAGTTA 
ATGGACTCTC AGCTCATTCA GGTCACTATG CCCCTGCCTG GCTCTCAGCT GCGTTATGGT 
TCAGCTCAAC AACACCTCAT CCTCCCACAG TCCATCCAGC TGCAGCAGGG ACAGAACTTG 
TCAGTGGGAG CCCCACGCCG AATGCTACCA CCAGGGTCGC AGGCAGCTGT CATGACTGGC 
AGCAGAGAGG GTCCACAGAT GGAAATGAAA GGTTTTCAGT TCTCTGAGAA GCCCAATCAT 
TCCCCAGGCA TGTCTGGAGG TTCTTACAGA CCTGGCTCTG CTAGCCCCGG CGGGAAGCCC 
TCTGGTCCTG GAGGACCTGT AGGCCCTCTG CCTACACATT TTGCTCAGCA GGTCCCTCCT 
GCTCAGGGCA GCATGGTGAT GCACATGCGT CCCCCCACCA CAGGCCCCTT CCCCAACCCT 
ATTCAGAGAC CTGTTATGCA GGTCAATAAG CCCGTCATCA TCCGTTCCCC CCCTTACCCC 
AATCCTGGCC GTGACCCTCC CCACTCCATC CCTCCTTCAG CTTCCGAGCC CCCAGTTAAA 
GGGCAAGAGG ATGGCATGAA GAATAAAACA ATAAGAGATG TACGCAAGGC GGTGGGAGAG 
GGCAAAACAC CGTCCGGGGG CATGACCAGC AAACTCCAGG AGCCCCTACC TTCCACAGGC 
CAAGCCAAAC CAGCACGCAC TGGAGCCATC AAACCCCAGG CTGTCAAAGT AGAAGAGGGC 
AAGGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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prrc2b cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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