ORF » Species Summary » Leishmania major strain Friedlin » LMJF_04_0650 cDNA ORF clone

LMJF_04_0650 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_04_0650 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_04_0650 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb00090 XM_883465.1
Latest version!		 Leishmania major strain Friedlin serine-threonine protein kinase-like protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_04_0650 ( XM_883465.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_888558.1 Leishmania major strain Friedlin serine-threonine protein kinase-like protein partial mRNA.
    Price & Availability↑
    CloneID OLb00090
    Clone ID Related Accession (Same CDS sequence) XM_883465.1
    Accession Version XM_883465.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product serine-threonine protein kinase-like protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007245). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAACGCAC CGGTGCATGA ACGCAGTGGC ACCAGCAGCA ACAGCGTGCC ACCTGCGCAC 
    AAGAAGCAGC GCTATGAAGG TGGGTGTAGT GGCAAGGTCC TTCACGACCG TGACGCGGAT 
    AGTGTGTCTG TCTGGGGTGT CCTCGTTGCG CAGAACCTGC CGTCGAGAGA AAACGGGCTG 
    CTGGCACACG CATCGCGTTG CCGCTGCCCT GTGGAAGGGG CAGACGTGCC GCGGAAAGAG 
    TCAGTGACGA AGCTGATAGC CTCATCGTCC CTGTGCAACC CTGGAGAAGA CTGGTTGTGG 
    CGGCACGTTC CACCAGCTCT ACGGCAGCTC TACTTTCCGG CGTGCTCCCA CAGCGCCAGC 
    ACGTACCCCT GCGCATCTGC GTCCGCGGAG TGTTCTGAGA AGTGTTGGAC GGCCTCGCTG 
    CAGCAGGAGG GAGACAAGTC TCACCCCTCA CCACCTCGCC TTCTCCTGCC CCTGTGCAGC 
    ACTCGCAGCA ACAACGGCGC TGTCCGCCTC GGTCGCCTCG CCAGCAACGA TATCGTCTTC 
    GCCACGAATC CGTGTGTCAG CAATGTGCAC TGCACCATCG CCTACCAGCC GGAAAGTGAT 
    CCTCGTCGCG GTGACGCGGC GGCGGCGCAA GACAAGGAGG CACAGAGGGC CGCCTTCGAG 
    GCGACCGGCG CCGTGGCCAA GGTCGTGGAT CCGCTTCATC ACAGTCCTCG ACCCGCAACG 
    GAGGCGCCAG CTGTGCCGCA TCCACGCGGC GATGCAACCG GCCGCAACTC CGCCGCCGCC 
    GCTCACGTTG TGCTCTCGGA CTCCAGCACA AACGGGTGCA TCGTCAACGG CCAGAAGGTG 
    GGCAAAGGCC GCTCGGTGCG CCTGCGCGAC GGCGACGTTG TGGAGCTCAT CAACGCCGGC 
    ACGCGGCACA CAACGGACTA CAACCTCGCG TTTCTCTTCC TCACGGCGGA GGTGTTTGTG 
    CGTTGGGTGG CACAGCAGCC GCATCAGGCG AGCACGACGC AAGGTGCCGA TGAGGAGGAC 
    ATCGCCTCGG CGGAGAAGGC TGCGAAGAAG GAGCATCAGC AACGTCAAGC ACTGCCGACA 
    GCTGCGCGAC GGCATGCGCT GCTGTGCAGA GCGCAGTGGA ACGTCGAGGC GCAGGTGCGG 
    CGCATGTACG GTCACAGCGT TGACGAGTAC TACTCCCTTG ATCGGGCACA TCCGCTGGGG 
    CAGGGAACGT TCGGCACCGT GTACCGTGCT GCCTTGCGCG CAGAGGCGTC AGCCTCATCG 
    CAGTCACCGT CCCTCCTCGA CGCTGGTGTT GGTGTCGCGG CTGGCGTGCC CTCAAGCTGG 
    GAGTACGTCT TCAGCGGCGA CGGCGTCGTT GAGTGGATGG CGAACCCGGC AGAGGTGACT 
    GAGCTACGGG AAGCCTACAT ACGGGGGAAG GAGCAACGGG CCCCGGCCGC GGTGCTGCAT 
    GTGTCCGCGT CGGCGGCCTC GACAACAGCA GCGGAGGCGG ACGCAACCAC TACAGATGAT 
    GCTGCGCCAC AGGTGTTCGC CGTCAAGATC ATCCGCAAGC AGCGCATGTT GGTGGAAGCT 
    CTTCGGCAGC AGCAGCAGCA GCAGCAGCAT CTCGAGGAAG GCGAAGGCGG TACGGATCGA 
    GCACCTGTGT CGTGCATCCA CAACGCCACC ACCGTCGCCA CCCCTGATAC TGCGGAGGCA 
    GTGCCAGGGA CGATATCGAC CGCAGATGCC GCCGTGATTG AGCAGCTGCT GCTCATGGAG 
    ACGCAGCCGG ACGATCTGGC CGCGCAGAGA CGGAAGGAGT GGACAGCAGG TGTGCTTGCC 
    AGTCTTCAGC TTGCCGACTC AACGCCCGCT GCCCTGGCGC CGTCCTCCTC GCTCAGCGCA 
    TTGCGGAAGT CGCAGCACAA GCCGCGAGAC GACGACGACG ACTCGAGTGA TGACGGGGAC 
    GCACAGCCAC TGCCGCAGCA AGATCGTGAG CAACGCCGTC GAGAGCTGAT CCGCTGCCTC 
    CCCAAGGCAC TCCGCCGCAC ATATGAGAAA GAGCTGCAGC ACCGCCGCCG TCAGCAGTGC 
    GAGATCAACA TTCTCCTCGC CGTGCGTCAC CGCAACATCA CCGCATTGTA CGAGGTGTTT 
    GACCAGCCAG ACCACCTGGC ATTGGTGATG GAGCAGGCGA CCGGAGGGGA AGTGTGGGAC 
    CTGCTGCAGA TCTACAAGCG ACGTGAGCGC AGGGGGCAAA CAGGTGCTCG TAAGAGCGAG 
    CGTGGCGCGA ACAACCATCG TGACACCGGT GCGCAACGGC TCGGCGACGG CAAGAGCGAC 
    TCGACAACGG CAGCGGCGAC TGCAGCTACG TGCGACGACG ACGACGACGT TGAACTCGTC 
    TCGGTCGGCG GCCCGCTGCC GGAGTTCATG GTGAAGATCA TTATCGTTCA AGTGATTGAG 
    GCAGTGTTGT ACCTGCACAC GATGGGCATC ATCCACCGTG ACTTGAAGCT CGAGAATTTG 
    ATGCTGCAGC GCCCCTGCGA CCGCTACGCG CTCAACGCGC TGCAGCTGCA GACGTTGGTT 
    CATCAGCTCC GCGCCTACTC TCGCCCGCAC CATGCAAGCG ATGCGTCCAC ATCCGCCGCG 
    ACGACGTGTG AGGAGGACAG TGAGGCGGTG GAGTTCACAA ATCCGCTGTC GGTTCTGTAC 
    ACGGTGCACG TACCGCGGCA CATGTGGCCG GTCGTCAAGG TCATGGATTT CGGTCTCTCG 
    CATGTGCTCG ACCAGCTGCA GACACATCCG TTCGACGCCG CCGGGGCGCC GCACGGCTTC 
    ATCGGGACGC TCTCGCAGAT GTCACAGGGC GTCGAAGGCA GCTCTGCTGC AGCGGCAGCA 
    GCAGCAGCCA GGGAGGCGGT GCCGGAGCGG GTGAAGCTAA TCTACTCCCG CAACGACGCA 
    ACGACGAGCT GCGGCACCCC TATCTACGCA GCCCCTGAGG TCACCAACCC GGCCTTGCGC 
    CCGGACAAGA TGGGGTACGG TGCAGCAGTC GACATGTACT CCGTCGGCGT CATCGCGTAC 
    GCGCTGCTGA CGGGGCGGGC ACCGTTTCCG TCAGCGAAAA ACCCGCGGCG GCCGGGCGGG 
    CCACCGGTGG TGAACTATGA CGCCCCCCTG CGCGTCCAGC GGCACCGTCG CCGCCCAGCC 
    AGTGCTGGGC CGCTCCCATC GTCACGGCCG TCCAGCTCTG CGGCACGCCC TCCTTCTGAG 
    GTCGCGTGTC TACCTCCGCT GTCGGTGGCA GAGTCCGTGC GCGTGGTGCT GCCGAAAGAA 
    CGTGCCGAGC TGTCAGCTGC GTCTTCCACC GCCTCGGAGC AGACTCGGCA GTGGCGCCGG 
    CGCGCCGAGG CAGTGGCGGC ATCTGTGCGG TGTGCGGAGC AGAAGGGTGC CCATGCGGAG 
    GGCAGCGATG GCGGCAGCAG TGCAACGCTG GCTGACCCGC TCTTTGCCAC CACCGTGGAG 
    CAGCTCTGCG CATCACTCAC CGCATACGCC GACATGTGTG CGTCGGGTGG GCTGGAGGTG 
    GACCTCGATC GCCTCGTGTA CGTCTCCGCG GACGCTGCCG CGACCGGTGC ACAGGCAAAC 
    GCGTCCGTCG GTCGCGTGAT GGCGCCTGAT GGGCACGTCG CGGATGTGTT GCTACCTCCC 
    ATCTCGGCGC TCGGCACATC CTTCCTTCGC GGACTTCTGG AGAAGTACCC TTCGCGGCGG 
    CTGACGGCTT ACGAGGCGCT GCAGCATCCG TGGTTGCGCG AGTGCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_888558.1
    CDS1..3711
    Translation

    Target ORF information:

    RefSeq Version XM_883465.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin serine-threonine protein kinase-like protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_883465.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAACGCAC CGGTGCATGA ACGCAGTGGC ACCAGCAGCA ACAGCGTGCC ACCTGCGCAC 
    AAGAAGCAGC GCTATGAAGG TGGGTGTAGT GGCAAGGTCC TTCACGACCG TGACGCGGAT 
    AGTGTGTCTG TCTGGGGTGT CCTCGTTGCG CAGAACCTGC CGTCGAGAGA AAACGGGCTG 
    CTGGCACACG CATCGCGTTG CCGCTGCCCT GTGGAAGGGG CAGACGTGCC GCGGAAAGAG 
    TCAGTGACGA AGCTGATAGC CTCATCGTCC CTGTGCAACC CTGGAGAAGA CTGGTTGTGG 
    CGGCACGTTC CACCAGCTCT ACGGCAGCTC TACTTTCCGG CGTGCTCCCA CAGCGCCAGC 
    ACGTACCCCT GCGCATCTGC GTCCGCGGAG TGTTCTGAGA AGTGTTGGAC GGCCTCGCTG 
    CAGCAGGAGG GAGACAAGTC TCACCCCTCA CCACCTCGCC TTCTCCTGCC CCTGTGCAGC 
    ACTCGCAGCA ACAACGGCGC TGTCCGCCTC GGTCGCCTCG CCAGCAACGA TATCGTCTTC 
    GCCACGAATC CGTGTGTCAG CAATGTGCAC TGCACCATCG CCTACCAGCC GGAAAGTGAT 
    CCTCGTCGCG GTGACGCGGC GGCGGCGCAA GACAAGGAGG CACAGAGGGC CGCCTTCGAG 
    GCGACCGGCG CCGTGGCCAA GGTCGTGGAT CCGCTTCATC ACAGTCCTCG ACCCGCAACG 
    GAGGCGCCAG CTGTGCCGCA TCCACGCGGC GATGCAACCG GCCGCAACTC CGCCGCCGCC 
    GCTCACGTTG TGCTCTCGGA CTCCAGCACA AACGGGTGCA TCGTCAACGG CCAGAAGGTG 
    GGCAAAGGCC GCTCGGTGCG CCTGCGCGAC GGCGACGTTG TGGAGCTCAT CAACGCCGGC 
    ACGCGGCACA CAACGGACTA CAACCTCGCG TTTCTCTTCC TCACGGCGGA GGTGTTTGTG 
    CGTTGGGTGG CACAGCAGCC GCATCAGGCG AGCACGACGC AAGGTGCCGA TGAGGAGGAC 
    ATCGCCTCGG CGGAGAAGGC TGCGAAGAAG GAGCATCAGC AACGTCAAGC ACTGCCGACA 
    GCTGCGCGAC GGCATGCGCT GCTGTGCAGA GCGCAGTGGA ACGTCGAGGC GCAGGTGCGG 
    CGCATGTACG GTCACAGCGT TGACGAGTAC TACTCCCTTG ATCGGGCACA TCCGCTGGGG 
    CAGGGAACGT TCGGCACCGT GTACCGTGCT GCCTTGCGCG CAGAGGCGTC AGCCTCATCG 
    CAGTCACCGT CCCTCCTCGA CGCTGGTGTT GGTGTCGCGG CTGGCGTGCC CTCAAGCTGG 
    GAGTACGTCT TCAGCGGCGA CGGCGTCGTT GAGTGGATGG CGAACCCGGC AGAGGTGACT 
    GAGCTACGGG AAGCCTACAT ACGGGGGAAG GAGCAACGGG CCCCGGCCGC GGTGCTGCAT 
    GTGTCCGCGT CGGCGGCCTC GACAACAGCA GCGGAGGCGG ACGCAACCAC TACAGATGAT 
    GCTGCGCCAC AGGTGTTCGC CGTCAAGATC ATCCGCAAGC AGCGCATGTT GGTGGAAGCT 
    CTTCGGCAGC AGCAGCAGCA GCAGCAGCAT CTCGAGGAAG GCGAAGGCGG TACGGATCGA 
    GCACCTGTGT CGTGCATCCA CAACGCCACC ACCGTCGCCA CCCCTGATAC TGCGGAGGCA 
    GTGCCAGGGA CGATATCGAC CGCAGATGCC GCCGTGATTG AGCAGCTGCT GCTCATGGAG 
    ACGCAGCCGG ACGATCTGGC CGCGCAGAGA CGGAAGGAGT GGACAGCAGG TGTGCTTGCC 
    AGTCTTCAGC TTGCCGACTC AACGCCCGCT GCCCTGGCGC CGTCCTCCTC GCTCAGCGCA 
    TTGCGGAAGT CGCAGCACAA GCCGCGAGAC GACGACGACG ACTCGAGTGA TGACGGGGAC 
    GCACAGCCAC TGCCGCAGCA AGATCGTGAG CAACGCCGTC GAGAGCTGAT CCGCTGCCTC 
    CCCAAGGCAC TCCGCCGCAC ATATGAGAAA GAGCTGCAGC ACCGCCGCCG TCAGCAGTGC 
    GAGATCAACA TTCTCCTCGC CGTGCGTCAC CGCAACATCA CCGCATTGTA CGAGGTGTTT 
    GACCAGCCAG ACCACCTGGC ATTGGTGATG GAGCAGGCGA CCGGAGGGGA AGTGTGGGAC 
    CTGCTGCAGA TCTACAAGCG ACGTGAGCGC AGGGGGCAAA CAGGTGCTCG TAAGAGCGAG 
    CGTGGCGCGA ACAACCATCG TGACACCGGT GCGCAACGGC TCGGCGACGG CAAGAGCGAC 
    TCGACAACGG CAGCGGCGAC TGCAGCTACG TGCGACGACG ACGACGACGT TGAACTCGTC 
    TCGGTCGGCG GCCCGCTGCC GGAGTTCATG GTGAAGATCA TTATCGTTCA AGTGATTGAG 
    GCAGTGTTGT ACCTGCACAC GATGGGCATC ATCCACCGTG ACTTGAAGCT CGAGAATTTG 
    ATGCTGCAGC GCCCCTGCGA CCGCTACGCG CTCAACGCGC TGCAGCTGCA GACGTTGGTT 
    CATCAGCTCC GCGCCTACTC TCGCCCGCAC CATGCAAGCG ATGCGTCCAC ATCCGCCGCG 
    ACGACGTGTG AGGAGGACAG TGAGGCGGTG GAGTTCACAA ATCCGCTGTC GGTTCTGTAC 
    ACGGTGCACG TACCGCGGCA CATGTGGCCG GTCGTCAAGG TCATGGATTT CGGTCTCTCG 
    CATGTGCTCG ACCAGCTGCA GACACATCCG TTCGACGCCG CCGGGGCGCC GCACGGCTTC 
    ATCGGGACGC TCTCGCAGAT GTCACAGGGC GTCGAAGGCA GCTCTGCTGC AGCGGCAGCA 
    GCAGCAGCCA GGGAGGCGGT GCCGGAGCGG GTGAAGCTAA TCTACTCCCG CAACGACGCA 
    ACGACGAGCT GCGGCACCCC TATCTACGCA GCCCCTGAGG TCACCAACCC GGCCTTGCGC 
    CCGGACAAGA TGGGGTACGG TGCAGCAGTC GACATGTACT CCGTCGGCGT CATCGCGTAC 
    GCGCTGCTGA CGGGGCGGGC ACCGTTTCCG TCAGCGAAAA ACCCGCGGCG GCCGGGCGGG 
    CCACCGGTGG TGAACTATGA CGCCCCCCTG CGCGTCCAGC GGCACCGTCG CCGCCCAGCC 
    AGTGCTGGGC CGCTCCCATC GTCACGGCCG TCCAGCTCTG CGGCACGCCC TCCTTCTGAG 
    GTCGCGTGTC TACCTCCGCT GTCGGTGGCA GAGTCCGTGC GCGTGGTGCT GCCGAAAGAA 
    CGTGCCGAGC TGTCAGCTGC GTCTTCCACC GCCTCGGAGC AGACTCGGCA GTGGCGCCGG 
    CGCGCCGAGG CAGTGGCGGC ATCTGTGCGG TGTGCGGAGC AGAAGGGTGC CCATGCGGAG 
    GGCAGCGATG GCGGCAGCAG TGCAACGCTG GCTGACCCGC TCTTTGCCAC CACCGTGGAG 
    CAGCTCTGCG CATCACTCAC CGCATACGCC GACATGTGTG CGTCGGGTGG GCTGGAGGTG 
    GACCTCGATC GCCTCGTGTA CGTCTCCGCG GACGCTGCCG CGACCGGTGC ACAGGCAAAC 
    GCGTCCGTCG GTCGCGTGAT GGCGCCTGAT GGGCACGTCG CGGATGTGTT GCTACCTCCC 
    ATCTCGGCGC TCGGCACATC CTTCCTTCGC GGACTTCTGG AGAAGTACCC TTCGCGGCGG 
    CTGACGGCTT ACGAGGCGCT GCAGCATCCG TGGTTGCGCG AGTGCGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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