LINJ_27_0750 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_27_0750
Entrez Gene ID	10966230
Full Name	putative phosphatidylinositol (3,5) kinase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_003392610.1	XP_003392658.1	putative phosphatidylinositol (3,5) kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_27_0750 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_27_0750 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc07551	XM_003392610.1 Latest version!	Leishmania infantum JPCM5 putative phosphatidylinositol (3,5) kinase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_27_0750 ( XM_003392610.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_003392658.1 Leishmania infantum JPCM5 putative phosphatidylinositol (3,5) kinase partial mRNA.

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CloneID	OLc07551
Clone ID Related Accession (Same CDS sequence)	XM_003392610.1
Accession Version	XM_003392610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative phosphatidylinositol (3,5) kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009411). COMPLETENESS: incomplete on both ends.

ATGCCAACAG ATCGCTCTCT ATGGGTGGAG GACAAGTATG CACTGCGATG TCGCGGGTGT 
GGCAAGAAGT TCACGGTCTT TCGCCGTCGC CACCACTGCC GCAGGTGTGG TCAGGTATTC 
TGCTACGAGT GCCTCAACCC TCTACCAACA GCGGCCTCCA CACCACAAAA CATCGTCTTC 
AGCGTCTACC AGTGGTTTTC GCCGTCGCCG GCTACGCCGC TTAGCACGCC GACATCAGCC 
CAAGCGCGGA AGACTACTGA GAAAAATGAA GTTGTCGGCA GTAGCCGCGA CGGGGCTGCG 
GCGGCGGCGG GCTCTACCGC CGCCGCAGCG GAGCTTTCTA TGCGTCTGTG CTGTCGCTGC 
GCTGCCACCA TTGCAGAGAA CGCTGCGCGC GAAGCCTCGG TGTCGCATGA CGAGTTTGAA 
CCCTCTTCGC TTCCAGTCCA CAGCACCGCC CCATTGGGGC CGATGATGCC GTCGCCCCTT 
ATGCGGAGTG TGTCACTACC GTGCCCGCCG CTATCGCAGC TGAATACGCC ATTGCGACTC 
AGCGGGAGGG GCCTTCCTGA AGGGGCGCCA TCGCCCGCTC CACGGCGTAC CCCGCCATCC 
TTCGAAGCGG CGCGCCAGTC GGAAGCCGAA GGCCCCCCTT ACATGGCCCC CGAGTTGCTG 
GAAGGCACAG CAATGCATGC GGAGACAGCA GAGGAGCGCG AGGTCACGTA CTCCATCGGC 
CGCGTTCGGC GTCCACCGCC GTCGGTCATG ACGCAGTGGT GGAAAGCTCA TCAAGAGGCT 
CTGACCAACA AGGAAGCGCG CACCTTCCCG GTGCAGCGGA CAGGCCCGTG CGCATCTACG 
TTGGAGAGTG CCGGAGCACT TGTGCGGACG CTGAGCGGCC ACGCCGACTT TCACGCGCGC 
TTCTTCCTGG AAGAAGATGT GACGCAGTCA CCATCGGACC CCAATGGTGG CGTGATGGGG 
AGCGCAGCGG TCGTTCCTGT TGCTGCCGCT CCGGCGCCGC CGCGCGGATT CCTGGACGAC 
TTCGGCGCTG TATGCACGAC CCACCTCGAG GCGCGTATTC AGCACATGCT TCAATCTTAT 
TTCCCTAACC TTGGCGGACC GCGACTGTGT TGCCACCTCG CTGATGTTGC CTGGCACATC 
GTGTGCCACA CTGCTGTCGC CCTCGACGCC AATATTTTGG ACCACTTGTC CAGCTTCTTC 
ATCCTGGACC CCGCGCAGCC GGCACAGTGT ATCGTGTACC CGGGCCTGGT AAACCGTCGA 
CGTCTGCCTT CGAAGCGAGT CATGAGCCCG TGCGACCATC CGCGCGTGCT GCTGCTCGCG 
GGGCACCTCT CCTACCCAGT GCAGCCGGCC GAAGACCTCG TTGAATATGT GCGTTCTTAC 
AGCGGCTACC TGGACAAGTT GTTCCAGCGG CTGGTGATGT GGCACCCCGA CGTGATTGTT 
GTCGAGGGCG GCATGCACCA TTACCTGCGC GCGCGCATCG AGGAGGAGGG CCAGATGCGC 
CTCGTGCTGG ACGTTGGTCG TGACTTTCTC GTCCAGCTAT CATGGTGCTT GCGTGCTGAC 
ATCATCGCTG ACCTTCAGTA CGTTGGCGTT GGCGACTTGG CCACCACAGC GCCGCTGGGT 
CACTGCACCC GCTTCGAGGT GCTCGAGTTG GCAGAGGAAG TGCACTGTTG CGGCTTCCGC 
GGCTTCGACG CGCTGTCGTT CCACACCCTC GTCCTCCGCG GCGGTTGTCC GAGCGGGGGC 
GTCGTTGCGC ACGACAGCAG CGACGGCGAG CAGCAGTGGG AAACAATGGA GAAGGTGGTA 
AGGGAGGCAG TTGTCGTTGC CTATCATCTG GCTCTGCAGG CGCACGGCGC CGCTGCACTT 
GTACGCAAAG GTCTGCCTGT TTCCGTGAGT GGTGCAGCGC CGACCGCAGA GGAAGCCGCC 
GCCGCCGCCA TCACAATGAA TCTCGGATGC CACTTCCCGT CGTCGGTACA GTCCGCGTGC 
CGCGACGCCG CGAGTCCCGC TGCGCTGTCT GCCCTGAAGG ACAACATCGT GGTGAACATT 
GTGCACATGG ATGAGCTATT CGAGGGGGGA TCCGGCGGCG GTGGCGGAGG TGCCGCCGTG 
AGCACACTGG CCAAGTCGCG GAGCACCGAG CTGTCCCTCG AGCGCGGCGC CTCTCGTGGC 
TCGCTCACGC TTTTCCAAGG TGGCTCACAC CTCGACTTAC CTGCCAGCGG CGCTGCATCA 
GCGTCACCCA GCGCTTCGCT GCTAAGCCCG CCTCTCGGAC GCGCTGAGGC GCCAGCGACG 
AATGCCTACG CGGTGGTGCA GCAGAAATCC GCCTTGACCT TCTACGGTTC CGGAGACGAG 
AGCCTCTTTC ACTTTCTGGT GAACCGCTGC GCCGGCATGG AGTCGCAGGT GCTCTACTTG 
CACGGCGCGC ATCGAGTGAA GGTGATCACG TCGACCAGTG CACTTCTCCC CTCTGCAGCA 
GCCATACTCA CTGAGAGCGG TCTCGCGGGC GTCTTGGGTG CTGCGCAGAT GCTGGCGTTT 
CACAAGGTTG CATCGCGGCA GAGCGCTGCG AGGGCGCTGG CCGCGGCGGA GCCGCTGGAC 
GCTCATGCAT TAGCCGCCTT TGTTGCGCAC CTGAAAGGAT ACTTTTCTCT TCGAATCCGC 
ATGGCGGACG AGGATGCAGG GGTAGCGGCG GCGGATGCGG TGGTCGTGGC TGCCGCCCCG 
GTCGACGCTG TGGTGGAGGT CTGCGAGCCG CATCTCCTCA ACCTCTCCAC CGCCGCCTTC 
CTGGAGTGGG TGGTGTACGG CTGGATACCC GCGCTCTCGA GGCAATTTGC GCAGCCGAGT 
GTGCAGTTGC ACTTCCAGTT CCATTCCCTA GGGATGGCTG CGCAGCGGCA GCAACCCGGT 
GCTACCAATG TCTCCGCCTA CGCTAATACC GTGACATTCC TGGTAGAGGC AGTGCCTCTG 
TTTCGCATCG AGTACCCGCG AACCGTGATG CCGCGCGCCG CTCCCGCACC ACAGGCAGAG 
GGTTCTTCTG AGGAGGATGC GATGAGCATC GTCGATTGTC TCTACGCCGG AGACGACGCC 
GAGGAAATGG AGGGTCTTCT CGCCGAGCTG CAGCAGGTGA CCCGCGCATC CTTGGATCGG 
ATGGAGCAGG TGGAGAACGC CAAGGCAAGT GTGCCGGCTG CAGCGTCCCC GGTAAACACT 
ACACACGTTC CAGAAGACGC GGAAGGAAAG GCAGCCGCCG CCGCCAGGCC GTCTTCGATG 
CCCACCGTGG AGCGCTCCAC GAGGGACTTG CACCAGCTCT CGGAAGACAT ACAGCACGCG 
CTCTCAGTCC TTCAAGATGC ACGCCAGCGT GGGCATCCGG ATGTGGTGCT TCTTCTTGCC 
AATATGCGCA GTCACCTGCT CCCCGCGCTG TTGGCCGCGT ATCGCGGCTG GCACGTGGCG 
CAGCGGAGGG CCACCACGAA GAGTGCCGCC ACTACCCCAG CAGACGCTGC CGCGGCTACC 
GCGCCTGAAT ACCCTTCTTG CCTTCGTCAG CTGGACGGAT GCCTGTATCA CAAGGAGCGT 
GCGCAGTGGA TTCGCCTGGC GGAGCCATCG AGCATCCTCG CCGCAGCCCT GCTTCACCTC 
TATCGGCCCC TTCCACCCAG CACGCCTTGC ACACCATTGA TCACAAAGGG TGCAGCTGCC 
GGCAGGGAGT TTGAACTGAA GAAGCCTGTC GGCAGCGAAG CAGCAGCGGC GGCATCGGCC 
ACACCCCATA TCGCCTTTGC TGTGGAATGC ACACACACTG CATCGTCGCC GGACATGAGC 
CCGGCCACGC GATCGGGGCA GCCGCCACTG CTGTCGATGG TAAGCCAGCT GTCACCAGCA 
GAGGCGCTCG CGGTGCTTCG CCATAGCGGC AGAGCAGATG CGGCATCGCC CACGTCGCTG 
AAGTGCACGC TCTCTGGGTA TCAGTCGGTG GTGCACCTCG GCGTCGGCGG ACCTGCTGGG 
CTCGCAGGTG GGGCGGTGGC GACGCTCAGC GGTGCCGCGA GTGTGGCCAG CATGATCGGT 
GGTGGCACTG GCAACGCGGA GCCCACCATC ACCGTGGACG TGCTCTTCCC CCTCAGCTTT 
GCAGCCCTTC ACGTGCTCTA CACGGACGGC GCTCCCTTTG ACATCTGTGC AGCGCTGGTG 
CGGTGTCGGC CCTTCAGCAC CGACGGCGGC AAGTCGCATT CGAGATTCTT TGTGACGCAG 
GACGGACGCT TTCTCATCAA GTCCGTGAAA CCGATGGAGC TGCGCCACTT CCGAGAGTGG 
GCACCCCGCT ACTTTGCAAG GATGGAAGAG CACTACACGT CGTTGCAGCG GGCGCAGCAG 
CCGCAGCCGT CTGACGGTGA GACCTGCGCG CACCTGTTTG AGGCGCAGTC AACGCTGGGA 
AAGATAATGG GCCTCTACAC CGTTCACGTG CAAGGTTCGC GCGGCAGAGC TTCGGCGTCG 
CCCGCCACGT CCTCCGGCGC AGGGCTTGGC GACACCACAC CGCAGCTGTG GGGCCTCCTC 
ACGGACGGAA CGCACTGCTT CATGGTGGTC GAGCAGCTTC TTTTTCAGCG ACCAGTGCGG 
GAGAAGTGGG ACCTGAAGGG CAGCCAGCGC AACCGCACGA CAGAGCAGAC GGCTGCGGTG 
CGCCTTGACG TCGACCTTGT TCAAGAGCGT CTACGGCTGG GCAACTTCTT CTTTTGCACG 
CCGGAGGCGA AGAGTCTATT GATGGACCAC CTCTCCCGCG ACACCGCGCT GCTGGCTGAC 
AGCGGCATCA TGGACTACTC GCTCATGGTC TCTGTCGGCG ACGGCAGCGT GTGCGTGGGC 
ATGATCGACT TTTTGCACCC GTACTCATCC GCTAAGGTGC TCGAGTCGAA GATGAAGTCT 
AGCCTAGACA CAATGCTCGG CTACACCCGC CGTGACCCAA CCATCATCGA TCCAGCAAGC 
TACGCGGCGC GGTTTATGCT CTGGATGGAT GGCTACTTCA ACGGCGTCCC AGATCGGCTG 
TTCCCGCTTA CCAGTGTGCG GCAGCTGAAG GAAGACCAGG AAAGCGGTGT GGTCGAAAAG 
CGGCCCCACC TCCCACCTCT GTCCAGTGAG ACAGCGAAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003392658.1
CDS	1..5085
Translation	MPTDRSLWVEDKYALRCRGCGKKFTVFRRRHHCRRCGQVFCYECLNPLPTAASTPQNIVFSVYQWFSPSPATPLSTPTSAQARKTTEKNEVVGSSRDGAAAAAGSTAAAAELSMRLCCRCAATIAENAAREASVSHDEFEPSSLPVHSTAPLGPMMPSPLMRSVSLPCPPLSQLNTPLRLSGRGLPEGAPSPAPRRTPPSFEAARQSEAEGPPYMAPELLEGTAMHAETAEEREVTYSIGRVRRPPPSVMTQWWKAHQEALTNKEARTFPVQRTGPCASTLESAGALVRTLSGHADFHARFFLEEDVTQSPSDPNGGVMGSAAVVPVAAAPAPPRGFLDDFGAVCTTHLEARIQHMLQSYFPNLGGPRLCCHLADVAWHIVCHTAVALDANILDHLSSFFILDPAQPAQCIVYPGLVNRRRLPSKRVMSPCDHPRVLLLAGHLSYPVQPAEDLVEYVRSYSGYLDKLFQRLVMWHPDVIVVEGGMHHYLRARIEEEGQMRLVLDVGRDFLVQLSWCLRADIIADLQYVGVGDLATTAPLGHCTRFEVLELAEEVHCCGFRGFDALSFHTLVLRGGCPSGGVVAHDSSDGEQQWETMEKVVREAVVVAYHLALQAHGAAALVRKGLPVSVSGAAPTAEEAAAAAITMNLGCHFPSSVQSACRDAASPAALSALKDNIVVNIVHMDELFEGGSGGGGGGAAVSTLAKSRSTELSLERGASRGSLTLFQGGSHLDLPASGAASASPSASLLSPPLGRAEAPATNAYAVVQQKSALTFYGSGDESLFHFLVNRCAGMESQVLYLHGAHRVKVITSTSALLPSAAAILTESGLAGVLGAAQMLAFHKVASRQSAARALAAAEPLDAHALAAFVAHLKGYFSLRIRMADEDAGVAAADAVVVAAAPVDAVVEVCEPHLLNLSTAAFLEWVVYGWIPALSRQFAQPSVQLHFQFHSLGMAAQRQQPGATNVSAYANTVTFLVEAVPLFRIEYPRTVMPRAAPAPQAEGSSEEDAMSIVDCLYAGDDAEEMEGLLAELQQVTRASLDRMEQVENAKASVPAAASPVNTTHVPEDAEGKAAAAARPSSMPTVERSTRDLHQLSEDIQHALSVLQDARQRGHPDVVLLLANMRSHLLPALLAAYRGWHVAQRRATTKSAATTPADAAAATAPEYPSCLRQLDGCLYHKERAQWIRLAEPSSILAAALLHLYRPLPPSTPCTPLITKGAAAGREFELKKPVGSEAAAAASATPHIAFAVECTHTASSPDMSPATRSGQPPLLSMVSQLSPAEALAVLRHSGRADAASPTSLKCTLSGYQSVVHLGVGGPAGLAGGAVATLSGAASVASMIGGGTGNAEPTITVDVLFPLSFAALHVLYTDGAPFDICAALVRCRPFSTDGGKSHSRFFVTQDGRFLIKSVKPMELRHFREWAPRYFARMEEHYTSLQRAQQPQPSDGETCAHLFEAQSTLGKIMGLYTVHVQGSRGRASASPATSSGAGLGDTTPQLWGLLTDGTHCFMVVEQLLFQRPVREKWDLKGSQRNRTTEQTAAVRLDVDLVQERLRLGNFFFCTPEAKSLLMDHLSRDTALLADSGIMDYSLMVSVGDGSVCVGMIDFLHPYSSAKVLESKMKSSLDTMLGYTRRDPTIIDPASYAARFMLWMDGYFNGVPDRLFPLTSVRQLKEDQESGVVEKRPHLPPLSSETAKR

Target ORF information:

RefSeq Version	XM_003392610.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative phosphatidylinositol (3,5) kinase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003392610.1

ORF Insert Sequence:

ATGCCAACAG ATCGCTCTCT ATGGGTGGAG GACAAGTATG CACTGCGATG TCGCGGGTGT 
GGCAAGAAGT TCACGGTCTT TCGCCGTCGC CACCACTGCC GCAGGTGTGG TCAGGTATTC 
TGCTACGAGT GCCTCAACCC TCTACCAACA GCGGCCTCCA CACCACAAAA CATCGTCTTC 
AGCGTCTACC AGTGGTTTTC GCCGTCGCCG GCTACGCCGC TTAGCACGCC GACATCAGCC 
CAAGCGCGGA AGACTACTGA GAAAAATGAA GTTGTCGGCA GTAGCCGCGA CGGGGCTGCG 
GCGGCGGCGG GCTCTACCGC CGCCGCAGCG GAGCTTTCTA TGCGTCTGTG CTGTCGCTGC 
GCTGCCACCA TTGCAGAGAA CGCTGCGCGC GAAGCCTCGG TGTCGCATGA CGAGTTTGAA 
CCCTCTTCGC TTCCAGTCCA CAGCACCGCC CCATTGGGGC CGATGATGCC GTCGCCCCTT 
ATGCGGAGTG TGTCACTACC GTGCCCGCCG CTATCGCAGC TGAATACGCC ATTGCGACTC 
AGCGGGAGGG GCCTTCCTGA AGGGGCGCCA TCGCCCGCTC CACGGCGTAC CCCGCCATCC 
TTCGAAGCGG CGCGCCAGTC GGAAGCCGAA GGCCCCCCTT ACATGGCCCC CGAGTTGCTG 
GAAGGCACAG CAATGCATGC GGAGACAGCA GAGGAGCGCG AGGTCACGTA CTCCATCGGC 
CGCGTTCGGC GTCCACCGCC GTCGGTCATG ACGCAGTGGT GGAAAGCTCA TCAAGAGGCT 
CTGACCAACA AGGAAGCGCG CACCTTCCCG GTGCAGCGGA CAGGCCCGTG CGCATCTACG 
TTGGAGAGTG CCGGAGCACT TGTGCGGACG CTGAGCGGCC ACGCCGACTT TCACGCGCGC 
TTCTTCCTGG AAGAAGATGT GACGCAGTCA CCATCGGACC CCAATGGTGG CGTGATGGGG 
AGCGCAGCGG TCGTTCCTGT TGCTGCCGCT CCGGCGCCGC CGCGCGGATT CCTGGACGAC 
TTCGGCGCTG TATGCACGAC CCACCTCGAG GCGCGTATTC AGCACATGCT TCAATCTTAT 
TTCCCTAACC TTGGCGGACC GCGACTGTGT TGCCACCTCG CTGATGTTGC CTGGCACATC 
GTGTGCCACA CTGCTGTCGC CCTCGACGCC AATATTTTGG ACCACTTGTC CAGCTTCTTC 
ATCCTGGACC CCGCGCAGCC GGCACAGTGT ATCGTGTACC CGGGCCTGGT AAACCGTCGA 
CGTCTGCCTT CGAAGCGAGT CATGAGCCCG TGCGACCATC CGCGCGTGCT GCTGCTCGCG 
GGGCACCTCT CCTACCCAGT GCAGCCGGCC GAAGACCTCG TTGAATATGT GCGTTCTTAC 
AGCGGCTACC TGGACAAGTT GTTCCAGCGG CTGGTGATGT GGCACCCCGA CGTGATTGTT 
GTCGAGGGCG GCATGCACCA TTACCTGCGC GCGCGCATCG AGGAGGAGGG CCAGATGCGC 
CTCGTGCTGG ACGTTGGTCG TGACTTTCTC GTCCAGCTAT CATGGTGCTT GCGTGCTGAC 
ATCATCGCTG ACCTTCAGTA CGTTGGCGTT GGCGACTTGG CCACCACAGC GCCGCTGGGT 
CACTGCACCC GCTTCGAGGT GCTCGAGTTG GCAGAGGAAG TGCACTGTTG CGGCTTCCGC 
GGCTTCGACG CGCTGTCGTT CCACACCCTC GTCCTCCGCG GCGGTTGTCC GAGCGGGGGC 
GTCGTTGCGC ACGACAGCAG CGACGGCGAG CAGCAGTGGG AAACAATGGA GAAGGTGGTA 
AGGGAGGCAG TTGTCGTTGC CTATCATCTG GCTCTGCAGG CGCACGGCGC CGCTGCACTT 
GTACGCAAAG GTCTGCCTGT TTCCGTGAGT GGTGCAGCGC CGACCGCAGA GGAAGCCGCC 
GCCGCCGCCA TCACAATGAA TCTCGGATGC CACTTCCCGT CGTCGGTACA GTCCGCGTGC 
CGCGACGCCG CGAGTCCCGC TGCGCTGTCT GCCCTGAAGG ACAACATCGT GGTGAACATT 
GTGCACATGG ATGAGCTATT CGAGGGGGGA TCCGGCGGCG GTGGCGGAGG TGCCGCCGTG 
AGCACACTGG CCAAGTCGCG GAGCACCGAG CTGTCCCTCG AGCGCGGCGC CTCTCGTGGC 
TCGCTCACGC TTTTCCAAGG TGGCTCACAC CTCGACTTAC CTGCCAGCGG CGCTGCATCA 
GCGTCACCCA GCGCTTCGCT GCTAAGCCCG CCTCTCGGAC GCGCTGAGGC GCCAGCGACG 
AATGCCTACG CGGTGGTGCA GCAGAAATCC GCCTTGACCT TCTACGGTTC CGGAGACGAG 
AGCCTCTTTC ACTTTCTGGT GAACCGCTGC GCCGGCATGG AGTCGCAGGT GCTCTACTTG 
CACGGCGCGC ATCGAGTGAA GGTGATCACG TCGACCAGTG CACTTCTCCC CTCTGCAGCA 
GCCATACTCA CTGAGAGCGG TCTCGCGGGC GTCTTGGGTG CTGCGCAGAT GCTGGCGTTT 
CACAAGGTTG CATCGCGGCA GAGCGCTGCG AGGGCGCTGG CCGCGGCGGA GCCGCTGGAC 
GCTCATGCAT TAGCCGCCTT TGTTGCGCAC CTGAAAGGAT ACTTTTCTCT TCGAATCCGC 
ATGGCGGACG AGGATGCAGG GGTAGCGGCG GCGGATGCGG TGGTCGTGGC TGCCGCCCCG 
GTCGACGCTG TGGTGGAGGT CTGCGAGCCG CATCTCCTCA ACCTCTCCAC CGCCGCCTTC 
CTGGAGTGGG TGGTGTACGG CTGGATACCC GCGCTCTCGA GGCAATTTGC GCAGCCGAGT 
GTGCAGTTGC ACTTCCAGTT CCATTCCCTA GGGATGGCTG CGCAGCGGCA GCAACCCGGT 
GCTACCAATG TCTCCGCCTA CGCTAATACC GTGACATTCC TGGTAGAGGC AGTGCCTCTG 
TTTCGCATCG AGTACCCGCG AACCGTGATG CCGCGCGCCG CTCCCGCACC ACAGGCAGAG 
GGTTCTTCTG AGGAGGATGC GATGAGCATC GTCGATTGTC TCTACGCCGG AGACGACGCC 
GAGGAAATGG AGGGTCTTCT CGCCGAGCTG CAGCAGGTGA CCCGCGCATC CTTGGATCGG 
ATGGAGCAGG TGGAGAACGC CAAGGCAAGT GTGCCGGCTG CAGCGTCCCC GGTAAACACT 
ACACACGTTC CAGAAGACGC GGAAGGAAAG GCAGCCGCCG CCGCCAGGCC GTCTTCGATG 
CCCACCGTGG AGCGCTCCAC GAGGGACTTG CACCAGCTCT CGGAAGACAT ACAGCACGCG 
CTCTCAGTCC TTCAAGATGC ACGCCAGCGT GGGCATCCGG ATGTGGTGCT TCTTCTTGCC 
AATATGCGCA GTCACCTGCT CCCCGCGCTG TTGGCCGCGT ATCGCGGCTG GCACGTGGCG 
CAGCGGAGGG CCACCACGAA GAGTGCCGCC ACTACCCCAG CAGACGCTGC CGCGGCTACC 
GCGCCTGAAT ACCCTTCTTG CCTTCGTCAG CTGGACGGAT GCCTGTATCA CAAGGAGCGT 
GCGCAGTGGA TTCGCCTGGC GGAGCCATCG AGCATCCTCG CCGCAGCCCT GCTTCACCTC 
TATCGGCCCC TTCCACCCAG CACGCCTTGC ACACCATTGA TCACAAAGGG TGCAGCTGCC 
GGCAGGGAGT TTGAACTGAA GAAGCCTGTC GGCAGCGAAG CAGCAGCGGC GGCATCGGCC 
ACACCCCATA TCGCCTTTGC TGTGGAATGC ACACACACTG CATCGTCGCC GGACATGAGC 
CCGGCCACGC GATCGGGGCA GCCGCCACTG CTGTCGATGG TAAGCCAGCT GTCACCAGCA 
GAGGCGCTCG CGGTGCTTCG CCATAGCGGC AGAGCAGATG CGGCATCGCC CACGTCGCTG 
AAGTGCACGC TCTCTGGGTA TCAGTCGGTG GTGCACCTCG GCGTCGGCGG ACCTGCTGGG 
CTCGCAGGTG GGGCGGTGGC GACGCTCAGC GGTGCCGCGA GTGTGGCCAG CATGATCGGT 
GGTGGCACTG GCAACGCGGA GCCCACCATC ACCGTGGACG TGCTCTTCCC CCTCAGCTTT 
GCAGCCCTTC ACGTGCTCTA CACGGACGGC GCTCCCTTTG ACATCTGTGC AGCGCTGGTG 
CGGTGTCGGC CCTTCAGCAC CGACGGCGGC AAGTCGCATT CGAGATTCTT TGTGACGCAG 
GACGGACGCT TTCTCATCAA GTCCGTGAAA CCGATGGAGC TGCGCCACTT CCGAGAGTGG 
GCACCCCGCT ACTTTGCAAG GATGGAAGAG CACTACACGT CGTTGCAGCG GGCGCAGCAG 
CCGCAGCCGT CTGACGGTGA GACCTGCGCG CACCTGTTTG AGGCGCAGTC AACGCTGGGA 
AAGATAATGG GCCTCTACAC CGTTCACGTG CAAGGTTCGC GCGGCAGAGC TTCGGCGTCG 
CCCGCCACGT CCTCCGGCGC AGGGCTTGGC GACACCACAC CGCAGCTGTG GGGCCTCCTC 
ACGGACGGAA CGCACTGCTT CATGGTGGTC GAGCAGCTTC TTTTTCAGCG ACCAGTGCGG 
GAGAAGTGGG ACCTGAAGGG CAGCCAGCGC AACCGCACGA CAGAGCAGAC GGCTGCGGTG 
CGCCTTGACG TCGACCTTGT TCAAGAGCGT CTACGGCTGG GCAACTTCTT CTTTTGCACG 
CCGGAGGCGA AGAGTCTATT GATGGACCAC CTCTCCCGCG ACACCGCGCT GCTGGCTGAC 
AGCGGCATCA TGGACTACTC GCTCATGGTC TCTGTCGGCG ACGGCAGCGT GTGCGTGGGC 
ATGATCGACT TTTTGCACCC GTACTCATCC GCTAAGGTGC TCGAGTCGAA GATGAAGTCT 
AGCCTAGACA CAATGCTCGG CTACACCCGC CGTGACCCAA CCATCATCGA TCCAGCAAGC 
TACGCGGCGC GGTTTATGCT CTGGATGGAT GGCTACTTCA ACGGCGTCCC AGATCGGCTG 
TTCCCGCTTA CCAGTGTGCG GCAGCTGAAG GAAGACCAGG AAAGCGGTGT GGTCGAAAAG 
CGGCCCCACC TCCCACCTCT GTCCAGTGAG ACAGCGAAGA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_27_0750 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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