Frem1 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene
Clones
gRNAs

Summary

Gene Symbol	Frem1
Entrez Gene ID	101965447
Full Name	FRAS1 related extracellular matrix 1
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005327999.1	XP_005328056.1	FRAS1-related extracellular matrix protein 1
XM_021728214.1	XP_021583889.1	FRAS1-related extracellular matrix protein 1

Pathways from BioSystems
Gene Ontology

The following Frem1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Frem1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc59043	XM_005327999.1 Latest version!	Ictidomys tridecemlineatus FRAS1 related extracellular matrix 1 (Frem1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OIc79342	XM_021728214.1 Latest version!	Ictidomys tridecemlineatus FRAS1 related extracellular matrix 1 (Frem1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Frem1 ( XM_005327999.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005328056.1 Ictidomys tridecemlineatus FRAS1 related extracellular matrix 1 (Frem1), mRNA.

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CloneID	OIc59043
Clone ID Related Accession (Same CDS sequence)	XM_005327999.1
Accession Version	XM_005327999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	FRAS1-related extracellular matrix protein 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936539.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAGCTCTC TAGGTCCGGG TGGCCTCAGT GTCCTCCTGC TTCTGCTGCT CCTGGACCGG 
GCCAGCCCTA CCTTCATCAG CCTGAACCAT GGGATGAGGG TGATGAAGGG CAGCTCTGCC 
TTCCTGTCGG AAGATGACCT CAAGTTTGCC ATCCCCAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC GATAACCCAG AGGGCAGGGA AACTCATGCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA TGAAGTCAGA TATGTTCACA ATGGTTGCCC GATTCTTGAT 
GAAGACACAG TGAAGCTTCG ACTTTACAGA TTCACTGAGA CAGACACCTT CACTGAAACC 
TTTACCCTGC GGGTCTATCT CATGGAGCCA GACTGCAATA TTATCCGCAT GAGTAGCAAT 
GTCCTGGAGG TGTCTGAATT CTACGGCCTG TCCCGAGCCA TTGATAAGAA CCTGCTCAGA 
TTCGACTATG AGAGGATGGT GGGCCTGGAG TGTACCGTCA GACTGGATCC TTTGAGAACT 
AGGCTGCCAG CTCACGGCCA GATGGTTGTG GTGGAGCCTC GCCCAGAAGG ACCCCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCTGAGTCC CAACTGGGAA TGGAACTGAA GTGTCCAGGT 
GGAAGCTGTA CCCTGGAATT GAAGAAAATA GCAAGTCTCC AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GGCTTCGTTA TCAACACCTG CAACCCCCTT CTCCCAACAT TGACTATATC 
TCCATCCAAC TGGACCTCAC AGACAGCAGG AGTCAAATCG TGTACAAGTC AGAGAGTGCG 
TGGTTGCCTG TCTACATCAG AGCTGGCATT CCGAACCAGA TTCCAAGGGC TGCGTTCATG 
GCCATGTTCA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CTTTGACTAC GTCAGTTCTG 
GACTGTGAAG AGGATGAGAC CCCCAAACCC TTGCTGGTGT TCAACATTAC AAAAGCCCCA 
CTCCAGGGCT ACATGACACA CCTGTTGGAT CACACCAGAC CCGTCTCCTC ATTCACCTGG 
AAGGATCTCA GCGACATGCA GATTGCCTAT CAGCCTCCTA ACAGCAGCCA TTCTGAGAGG 
AGAAATTATG AGGTGGAGTT GGAGGTATAT GACTTTTTCT TTGGAAGGAG TGCACCTGTC 
ACAGTCCATA TCTCCATCAG AACAGCAGAC ACCAACGCCC CCCGGGTGTC CTGGAATACA 
GGTCTGAATC TCCTGGAGGG ACAGTCTCGG GCTATCACTT GGGAACAGTT TCAGATTGTT 
GACAATGATG ACATTGGTGC CGTCCAGTTG GTCACTGTTG ATGGCCTGCA TCACGGACAG 
CTTACAGTGA GAGGGGGGAA AGGATTTCTC TTCACGGTGG CTGACCTTCA GACTGGAGTT 
GTTCGCTATC ACCATGACGA TAGCGACTCC ACTAAAGACT TTGTGGTCTT CAGGATCTTC 
GATGGCCATC ACAGCATCCG TCACAAGTTT CCCATCAACA TCTTGCCCAA GGATGACAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AAGGACAGAC CATCCTGGTG 
CAGGGTTCCA TGCTGAGAGC CTCAGACATG GACTCCAGTG ACGACTACAT CTTCTTCAAC 
ATCACAAAAC CCCCGCAGGC TGGAGAGATC ATGAAGAAGC CAGGGCCAGG GCTGATAGGC 
TATCCTGTCT CTGGCTTTCT TCAGAGGGAT CTGTTTAATG GGATCATTTA CTATCATCAC 
TTTGGTGGAG AAATCTTTGA AGATTCTTTC GAATTTGTCC TGTGGGACAG CCACGAACCT 
CCAAATCTCT CAGTGCCACA GGTGGTGACC ATCCATATCA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGGGT TTCTCGGCAT TTGGTTGTCA AGGAAACAGA GGTGGCCTAC 
ATAACGAAGA AGCATCTACA TTTCCTAGAT ACAGAATCTT ACGATAGGGA GCTGCTCTAC 
ACAATAACCA CTCCTCCCTG TTTCTCTTTC AATGACAGAT ATTTGGATGC TGGGAAATTA 
TTCATGGTGG ACAGCATACC AAAGCTAACC AAAAATCCTA CAGCCCTGGG GCTGAGGTCA 
TTTACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCACCTAT GCAAGATATA 
GGCCCTCATC TCCGACATGT CCAGTTTACC TTTTCCATCA GTAACCAGCA TGGTGGCACT 
TTGCACGGGA TCTGCTTTAA TATTACAATT CTCCCAGTGG ACAATCAAGT TCCTGAGGTA 
TCCACCACCC CTCTGAGAGT GGCTGAGGGA GGCCAGTGCG TCATCAGCAC AGAGCACATT 
CTGGTTTCTG ATGTGGATAC CATGTTGGAC AATATCTACA TTGCCCTGCA GGGTCTGCCT 
CTGCATGGAA GGCTGGAGTT GAATGGATTT CCTCTAGATG CTGGGGACAC ATTTTCTTGG 
GGAGACCTTC GTACCTTAAA AGTTAGGTAT CAGCATGATG GATCTGAGGT TCTTCAGGAT 
GACATACGTT TGGAGGTCAC AGATGGCACA AATTCAGCAG AATTTGTTTT ACACGTTGAG 
GTGTTTCCTA TAAATGATGA GCCACCGATC CTGAAGGCTG ACCTCATCCC CATGATGCAT 
TGCTCCGAGG GTGGAGAGGT GGTCATCACC CCTGAACACA TTTTTGCTAC TGATATGGAC 
AGTGATGACT TGAAACTGAT GGTTCTGATT GCCCGAGAAC CTCAGCATGG GGTGGTGTGG 
AAGGCTGGTG TTCAAGTAGA CCAGTTCTCT CAGGGAGATA TTATCTCAGG GGCTGTGACA 
TACAGACACA CAGGTGGTGA GATTGGCCTG GTGCCTTGTT TCGATACCAT TACTTTGGTG 
ATTTCTGATG GAGAAGCTGG CCCTTTTGTG AATGGCTGTT GTTACAGTGG ACCTAATCCA 
TCTGTTCCTC TCCATGAGTC CTTTCCAGTG TATGATCTCA ACATCACAGT ACATCCAGTA 
GACAATCAGC CACCTTCAAT TGCAATAGGT ACCTTGTTCC TTGTAGATGA GGGGTTCTCA 
GCAGCCCTTA CCATTAACCA TTTGTCTGCC AGTGATCCTG ACACTGCTGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCCCAATTT GGTTACCTTG AAAACACACT TCCTTCTGTG 
GGTTTTGAGA AAAGCAATAT GGGCATAAGT ATAGCTTCCT TTCACTGGAA AGACATGAAG 
GCTTTGCACA TCAACTATGT GCAATCCAGA CACCTGAGGA TGGAACCCAC CACTGACCAG 
TTTGTGGTGT ACGTCACAGA TGGGAAGCAA CGCTCCTTGC AGACTCCATT TTATGTTATA 
ATCAACCCCA CAAATGATGA AGTTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGACGA CAGAGCTGGA TTCTTCTGTC ATCAGTGCTG CCGACCTGGA CATTCCCAAG 
GACCTCTTAT TGTTCAGCAT CATTCAGAAA CCACGTCATG GTCTCCTCAT CAATAGGATG 
CTCAGCAAAG ACTCTCCTCA GAATAAGCAG CCAGCCAACC CTGACCAGAA GCAGGAACTT 
GTTCACAGCT TTTCCTTGGA ACTTTTCAAG ACTGGAATGA GGTTGACCTA TGTGCATGAT 
GACTCTGAGA GCTTTGCTGA TGACTTTACA ATCCAATTGT CAGATGGGAA ACACAAGATA 
CTTAAAATCA TTTCAGTAGA GATCACTCCG GTTAATGATG AGAAACCAAT GCTAAGCAAG 
AAGGCTGAAA TCGAAATGAA TATGGGGGAT ACCCGTATTA TTTCTAGCGC TGTTCTTTCC 
GCTGTAGACA AAGACTCACC CAGGGAGAAG ATTTACTATT TATTTGAAAG GCTTCCCCAA 
CATGGACAAC TTCAGCTTAA GATAGGGAGA GACTGGGTTT CTCTCTCCCC GGGCATGAAA 
TGCACCCAGG AAGAAGTGGA TCTGAATTTG CTGAGATACA CGCACACCGG GGCAATGGAT 
TCCCAGAATC GAGATAGCTT CACCTTTTAC CTCTGGGATG GTGACAACAG ATCACCTGCA 
TTTGACTGTC ACATCATCAT CAAGGATATG GGAAAAGGTT ATATTGTCCT TCTTGCAAAA 
CCACTTGTGG TATCTAAAGG CGACAGAGGG TTCTTGACGA CCGCCACTCT TCTGGCTGTG 
GATGGAACAG ACAGGCCTGA GGAACTGCTG TATGCCATTA CCTCTCCGCC GCGGTATGGC 
CAGGTGGAAT ATGTCCGCTA TCCCGGGGTC CCCATTACAA GCTTCAGCCA ATTGGACATA 
GCGGGGCAGA CAGTCTGCTA CATACATAAG AGTGGGGCAC CTGTGCCCAG TGACAGCTTC 
AGATTCATCG TCAGCAATGG ACTGTGGACC AAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTAGACA GAGCCTTGCC TGTGGTCACC AGGAACAAGG GGATGAGGCT GGCCGAGGGG 
GCCATGGGCC TGCTTTCTCC TGACCTCCTC CAGCTGACTG ACCCTGACAC TCCTGCCGAA 
AACCTCACCT TCCTCCTGGT CCAGCTCCCA CAGCACGGCC GGCTCTACCT GCGGGGAACT 
GTACTGCTTC GACACAACTT CACCCAGTGG GATGTGGACA GCGGGAATGT GGCCTATCAG 
CACTCTGGAG GCAGCTCCCA GATTGACCAC TTCACTTTCA TGGCCACAGA TAGGACAAAC 
CAAGGCTTTG TTGTGGATGG GAGGGTGCGA GAAGAGCCTG TTCCATTCAC CATTCAGGTG 
GAGCATTTGG ATAAAATAGC CCCCCGGATC ACACTCTTGC GTTCCCCTTC ACAAGTGAGA 
CTCCTGAAAA ACGGCTGTTA TGGGATTTAC ATCACTTCCC GAGTGTTGAA GGCATCGGAC 
CCTGACACCG AGGACCATCA GATAATCTTT AAGATTTTAC GAGGCCCACT ACACGGACAT 
TTGGAGAATA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTATAC 
AGTAAGACCA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TATCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCT GCCACTCCTC AAATTTTGGA GTTGAAGTGG 
TCTCATATTG AATGGTCACA AACTGAATAT GAGGTCTGTG AGAATGTGGG CATGTTGTCC 
TTAGAAGTTA CCAGAAGGGG ATATTCCATG GACTCAGCCT TTGTGGGAGT AAAGGTCAAT 
CAAGTGTCAG CTGCAGCTGG AAAAGATTTC ACCGTGACTC CATCCAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACGAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC ATTGCCATCC TTCATACTCC 
TCCAACCAAA GCAAGCCCAA CACATGGGAG AAGGGCATTT GGCGCCCGTT GCATCCAGGG 
TCTCCCTCGC CCACCACTTC TGGTTCCCTT CATCTGGAAA GAAGACCCCC TCCATCTTCC 
AAGCAGCTGG TGACCACCAG GGGAGACTCC CTACAGGGCT TTGACTCCAC AGCTCTTTCT 
CAGAGGAAGC TGAGGACCCA CTGGAACGGC AAAGCAGTTC ATCCATCTTC TGTTTATAGA 
AACAGAACAG ACATCATCTA TAATTATCAT GGGATAGCTT CCTTGAAACT GGAAGATGAC 
AGTGCCCCAC CTCCCCAAAG GAAGGCCAAA GTATCCATTA TTAGTCAGCC ACAAAAGACA 
ATGAAAGTGG CAGAGCTGCC TGCAGTAGAT AAGGTGGAAT CCACAACTGA CTCTCACTTC 
CCCAGACAGG ACCAGTTGCC CTTATTTCCA AAGAATTGCA CAATGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAAGCAC CCAAAAGCTG TATGGATGCA ATGGGATTGC CTGGAAATCC 
TGGAGCCCCC AAACCAAGGT GGGAAACAGA CACCTGGGAA ATCTTGTAAC TGTATTGTCC 
AGGCAGCACA TGCAGTGGCT CTGGGACATC AGTGGAAGAA AGCCCTTTTG GATAGGTTTG 
AATGACCAAG CACACGCTGG CCACTGGGAA TGGATTGGAG GCGAACCTGT CACCTTCACC 
AACTGGAGGA GAGAGCCCCC TCAATACTCA AAGCCTGGAA AGAACTGTGT TTTGGTTCAA 
AGAGGAGGGA AATGGCAAAC AAAGAACTGT ATGAAGGGCA AACCTCAAAA TTTCATGTGC 
TCCAGGAAAC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005328056.1
CDS	1..6435
Translation	MSSLGPGGLSVLLLLLLLDRASPTFISLNHGMRVMKGSSAFLSEDDLKFAIPKEKDACKVEVVMNEPITQRAGKLMPQVFDCHFLPNEVRYVHNGCPILDEDTVKLRLYRFTETDTFTETFTLRVYLMEPDCNIIRMSSNVLEVSEFYGLSRAIDKNLLRFDYERMVGLECTVRLDPLRTRLPAHGQMVVVEPRPEGPRGDQPHSFFPESQLGMELKCPGGSCTLELKKIASLQVSCEEFLLMGLRYQHLQPPSPNIDYISIQLDLTDSRSQIVYKSESAWLPVYIRAGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYMTHLLDHTRPVSSFTWKDLSDMQIAYQPPNSSHSERRNYEVELEVYDFFFGRSAPVTVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTVDGLHHGQLTVRGGKGFLFTVADLQTGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINILPKDDSPPFLITNVVIELEEGQTILVQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPGPGLIGYPVSGFLQRDLFNGIIYYHHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKHLHFLDTESYDRELLYTITTPPCFSFNDRYLDAGKLFMVDSIPKLTKNPTALGLRSFTQHAVNYMKVAYMPPMQDIGPHLRHVQFTFSISNQHGGTLHGICFNITILPVDNQVPEVSTTPLRVAEGGQCVISTEHILVSDVDTMLDNIYIALQGLPLHGRLELNGFPLDAGDTFSWGDLRTLKVRYQHDGSEVLQDDIRLEVTDGTNSAEFVLHVEVFPINDEPPILKADLIPMMHCSEGGEVVITPEHIFATDMDSDDLKLMVLIAREPQHGVVWKAGVQVDQFSQGDIISGAVTYRHTGGEIGLVPCFDTITLVISDGEAGPFVNGCCYSGPNPSVPLHESFPVYDLNITVHPVDNQPPSIAIGTLFLVDEGFSAALTINHLSASDPDTAADDLEFVLVSPPQFGYLENTLPSVGFEKSNMGISIASFHWKDMKALHINYVQSRHLRMEPTTDQFVVYVTDGKQRSLQTPFYVIINPTNDEVPDFVVQNITVCEGQTTELDSSVISAADLDIPKDLLLFSIIQKPRHGLLINRMLSKDSPQNKQPANPDQKQELVHSFSLELFKTGMRLTYVHDDSESFADDFTIQLSDGKHKILKIISVEITPVNDEKPMLSKKAEIEMNMGDTRIISSAVLSAVDKDSPREKIYYLFERLPQHGQLQLKIGRDWVSLSPGMKCTQEEVDLNLLRYTHTGAMDSQNRDSFTFYLWDGDNRSPAFDCHIIIKDMGKGYIVLLAKPLVVSKGDRGFLTTATLLAVDGTDRPEELLYAITSPPRYGQVEYVRYPGVPITSFSQLDIAGQTVCYIHKSGAPVPSDSFRFIVSNGLWTKHGVFEITLETVDRALPVVTRNKGMRLAEGAMGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGRLYLRGTVLLRHNFTQWDVDSGNVAYQHSGGSSQIDHFTFMATDRTNQGFVVDGRVREEPVPFTIQVEHLDKIAPRITLLRSPSQVRLLKNGCYGIYITSRVLKASDPDTEDHQIIFKILRGPLHGHLENTTTGEFIHEKFSQKDLYSKTILYIINPSLEVNSDIVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGMLSLEVTRRGYSMDSAFVGVKVNQVSAAAGKDFTVTPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGHCHPSYSSNQSKPNTWEKGIWRPLHPGSPSPTTSGSLHLERRPPPSSKQLVTTRGDSLQGFDSTALSQRKLRTHWNGKAVHPSSVYRNRTDIIYNYHGIASLKLEDDSAPPPQRKAKVSIISQPQKTMKVAELPAVDKVESTTDSHFPRQDQLPLFPKNCTMELKGLFHFEESTQKLYGCNGIAWKSWSPQTKVGNRHLGNLVTVLSRQHMQWLWDISGRKPFWIGLNDQAHAGHWEWIGGEPVTFTNWRREPPQYSKPGKNCVLVQRGGKWQTKNCMKGKPQNFMCSRKL

Target ORF information:

RefSeq Version	XM_005327999.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus FRAS1 related extracellular matrix 1 (Frem1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005327999.1

ORF Insert Sequence:

ATGAGCTCTC TAGGTCCGGG TGGCCTCAGT GTCCTCCTGC TTCTGCTGCT CCTGGACCGG 
GCCAGCCCTA CCTTCATCAG CCTGAACCAT GGGATGAGGG TGATGAAGGG CAGCTCTGCC 
TTCCTGTCGG AAGATGACCT CAAGTTTGCC ATCCCCAAAG AGAAAGATGC CTGCAAAGTG 
GAAGTTGTGA TGAATGAGCC GATAACCCAG AGGGCAGGGA AACTCATGCC ACAGGTCTTT 
GACTGCCATT TCCTTCCCAA TGAAGTCAGA TATGTTCACA ATGGTTGCCC GATTCTTGAT 
GAAGACACAG TGAAGCTTCG ACTTTACAGA TTCACTGAGA CAGACACCTT CACTGAAACC 
TTTACCCTGC GGGTCTATCT CATGGAGCCA GACTGCAATA TTATCCGCAT GAGTAGCAAT 
GTCCTGGAGG TGTCTGAATT CTACGGCCTG TCCCGAGCCA TTGATAAGAA CCTGCTCAGA 
TTCGACTATG AGAGGATGGT GGGCCTGGAG TGTACCGTCA GACTGGATCC TTTGAGAACT 
AGGCTGCCAG CTCACGGCCA GATGGTTGTG GTGGAGCCTC GCCCAGAAGG ACCCCGTGGA 
GATCAGCCAC ACAGTTTTTT CCCTGAGTCC CAACTGGGAA TGGAACTGAA GTGTCCAGGT 
GGAAGCTGTA CCCTGGAATT GAAGAAAATA GCAAGTCTCC AAGTGAGCTG TGAGGAGTTC 
CTGCTGATGG GGCTTCGTTA TCAACACCTG CAACCCCCTT CTCCCAACAT TGACTATATC 
TCCATCCAAC TGGACCTCAC AGACAGCAGG AGTCAAATCG TGTACAAGTC AGAGAGTGCG 
TGGTTGCCTG TCTACATCAG AGCTGGCATT CCGAACCAGA TTCCAAGGGC TGCGTTCATG 
GCCATGTTCA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CTTTGACTAC GTCAGTTCTG 
GACTGTGAAG AGGATGAGAC CCCCAAACCC TTGCTGGTGT TCAACATTAC AAAAGCCCCA 
CTCCAGGGCT ACATGACACA CCTGTTGGAT CACACCAGAC CCGTCTCCTC ATTCACCTGG 
AAGGATCTCA GCGACATGCA GATTGCCTAT CAGCCTCCTA ACAGCAGCCA TTCTGAGAGG 
AGAAATTATG AGGTGGAGTT GGAGGTATAT GACTTTTTCT TTGGAAGGAG TGCACCTGTC 
ACAGTCCATA TCTCCATCAG AACAGCAGAC ACCAACGCCC CCCGGGTGTC CTGGAATACA 
GGTCTGAATC TCCTGGAGGG ACAGTCTCGG GCTATCACTT GGGAACAGTT TCAGATTGTT 
GACAATGATG ACATTGGTGC CGTCCAGTTG GTCACTGTTG ATGGCCTGCA TCACGGACAG 
CTTACAGTGA GAGGGGGGAA AGGATTTCTC TTCACGGTGG CTGACCTTCA GACTGGAGTT 
GTTCGCTATC ACCATGACGA TAGCGACTCC ACTAAAGACT TTGTGGTCTT CAGGATCTTC 
GATGGCCATC ACAGCATCCG TCACAAGTTT CCCATCAACA TCTTGCCCAA GGATGACAGT 
CCCCCGTTCC TCATAACCAA TGTTGTGATT GAACTGGAGG AAGGACAGAC CATCCTGGTG 
CAGGGTTCCA TGCTGAGAGC CTCAGACATG GACTCCAGTG ACGACTACAT CTTCTTCAAC 
ATCACAAAAC CCCCGCAGGC TGGAGAGATC ATGAAGAAGC CAGGGCCAGG GCTGATAGGC 
TATCCTGTCT CTGGCTTTCT TCAGAGGGAT CTGTTTAATG GGATCATTTA CTATCATCAC 
TTTGGTGGAG AAATCTTTGA AGATTCTTTC GAATTTGTCC TGTGGGACAG CCACGAACCT 
CCAAATCTCT CAGTGCCACA GGTGGTGACC ATCCATATCA CTCCAGTGGA TGACCAGCTT 
CCAAAAGAGG CTCCTGGGGT TTCTCGGCAT TTGGTTGTCA AGGAAACAGA GGTGGCCTAC 
ATAACGAAGA AGCATCTACA TTTCCTAGAT ACAGAATCTT ACGATAGGGA GCTGCTCTAC 
ACAATAACCA CTCCTCCCTG TTTCTCTTTC AATGACAGAT ATTTGGATGC TGGGAAATTA 
TTCATGGTGG ACAGCATACC AAAGCTAACC AAAAATCCTA CAGCCCTGGG GCTGAGGTCA 
TTTACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCACCTAT GCAAGATATA 
GGCCCTCATC TCCGACATGT CCAGTTTACC TTTTCCATCA GTAACCAGCA TGGTGGCACT 
TTGCACGGGA TCTGCTTTAA TATTACAATT CTCCCAGTGG ACAATCAAGT TCCTGAGGTA 
TCCACCACCC CTCTGAGAGT GGCTGAGGGA GGCCAGTGCG TCATCAGCAC AGAGCACATT 
CTGGTTTCTG ATGTGGATAC CATGTTGGAC AATATCTACA TTGCCCTGCA GGGTCTGCCT 
CTGCATGGAA GGCTGGAGTT GAATGGATTT CCTCTAGATG CTGGGGACAC ATTTTCTTGG 
GGAGACCTTC GTACCTTAAA AGTTAGGTAT CAGCATGATG GATCTGAGGT TCTTCAGGAT 
GACATACGTT TGGAGGTCAC AGATGGCACA AATTCAGCAG AATTTGTTTT ACACGTTGAG 
GTGTTTCCTA TAAATGATGA GCCACCGATC CTGAAGGCTG ACCTCATCCC CATGATGCAT 
TGCTCCGAGG GTGGAGAGGT GGTCATCACC CCTGAACACA TTTTTGCTAC TGATATGGAC 
AGTGATGACT TGAAACTGAT GGTTCTGATT GCCCGAGAAC CTCAGCATGG GGTGGTGTGG 
AAGGCTGGTG TTCAAGTAGA CCAGTTCTCT CAGGGAGATA TTATCTCAGG GGCTGTGACA 
TACAGACACA CAGGTGGTGA GATTGGCCTG GTGCCTTGTT TCGATACCAT TACTTTGGTG 
ATTTCTGATG GAGAAGCTGG CCCTTTTGTG AATGGCTGTT GTTACAGTGG ACCTAATCCA 
TCTGTTCCTC TCCATGAGTC CTTTCCAGTG TATGATCTCA ACATCACAGT ACATCCAGTA 
GACAATCAGC CACCTTCAAT TGCAATAGGT ACCTTGTTCC TTGTAGATGA GGGGTTCTCA 
GCAGCCCTTA CCATTAACCA TTTGTCTGCC AGTGATCCTG ACACTGCTGC AGATGACTTG 
GAATTTGTTT TGGTTTCTCC TCCCCAATTT GGTTACCTTG AAAACACACT TCCTTCTGTG 
GGTTTTGAGA AAAGCAATAT GGGCATAAGT ATAGCTTCCT TTCACTGGAA AGACATGAAG 
GCTTTGCACA TCAACTATGT GCAATCCAGA CACCTGAGGA TGGAACCCAC CACTGACCAG 
TTTGTGGTGT ACGTCACAGA TGGGAAGCAA CGCTCCTTGC AGACTCCATT TTATGTTATA 
ATCAACCCCA CAAATGATGA AGTTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGTGAG 
GGTCAGACGA CAGAGCTGGA TTCTTCTGTC ATCAGTGCTG CCGACCTGGA CATTCCCAAG 
GACCTCTTAT TGTTCAGCAT CATTCAGAAA CCACGTCATG GTCTCCTCAT CAATAGGATG 
CTCAGCAAAG ACTCTCCTCA GAATAAGCAG CCAGCCAACC CTGACCAGAA GCAGGAACTT 
GTTCACAGCT TTTCCTTGGA ACTTTTCAAG ACTGGAATGA GGTTGACCTA TGTGCATGAT 
GACTCTGAGA GCTTTGCTGA TGACTTTACA ATCCAATTGT CAGATGGGAA ACACAAGATA 
CTTAAAATCA TTTCAGTAGA GATCACTCCG GTTAATGATG AGAAACCAAT GCTAAGCAAG 
AAGGCTGAAA TCGAAATGAA TATGGGGGAT ACCCGTATTA TTTCTAGCGC TGTTCTTTCC 
GCTGTAGACA AAGACTCACC CAGGGAGAAG ATTTACTATT TATTTGAAAG GCTTCCCCAA 
CATGGACAAC TTCAGCTTAA GATAGGGAGA GACTGGGTTT CTCTCTCCCC GGGCATGAAA 
TGCACCCAGG AAGAAGTGGA TCTGAATTTG CTGAGATACA CGCACACCGG GGCAATGGAT 
TCCCAGAATC GAGATAGCTT CACCTTTTAC CTCTGGGATG GTGACAACAG ATCACCTGCA 
TTTGACTGTC ACATCATCAT CAAGGATATG GGAAAAGGTT ATATTGTCCT TCTTGCAAAA 
CCACTTGTGG TATCTAAAGG CGACAGAGGG TTCTTGACGA CCGCCACTCT TCTGGCTGTG 
GATGGAACAG ACAGGCCTGA GGAACTGCTG TATGCCATTA CCTCTCCGCC GCGGTATGGC 
CAGGTGGAAT ATGTCCGCTA TCCCGGGGTC CCCATTACAA GCTTCAGCCA ATTGGACATA 
GCGGGGCAGA CAGTCTGCTA CATACATAAG AGTGGGGCAC CTGTGCCCAG TGACAGCTTC 
AGATTCATCG TCAGCAATGG ACTGTGGACC AAGCACGGGG TGTTTGAGAT CACACTGGAG 
ACTGTAGACA GAGCCTTGCC TGTGGTCACC AGGAACAAGG GGATGAGGCT GGCCGAGGGG 
GCCATGGGCC TGCTTTCTCC TGACCTCCTC CAGCTGACTG ACCCTGACAC TCCTGCCGAA 
AACCTCACCT TCCTCCTGGT CCAGCTCCCA CAGCACGGCC GGCTCTACCT GCGGGGAACT 
GTACTGCTTC GACACAACTT CACCCAGTGG GATGTGGACA GCGGGAATGT GGCCTATCAG 
CACTCTGGAG GCAGCTCCCA GATTGACCAC TTCACTTTCA TGGCCACAGA TAGGACAAAC 
CAAGGCTTTG TTGTGGATGG GAGGGTGCGA GAAGAGCCTG TTCCATTCAC CATTCAGGTG 
GAGCATTTGG ATAAAATAGC CCCCCGGATC ACACTCTTGC GTTCCCCTTC ACAAGTGAGA 
CTCCTGAAAA ACGGCTGTTA TGGGATTTAC ATCACTTCCC GAGTGTTGAA GGCATCGGAC 
CCTGACACCG AGGACCATCA GATAATCTTT AAGATTTTAC GAGGCCCACT ACACGGACAT 
TTGGAGAATA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTATAC 
AGTAAGACCA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TATCGTGGAA 
TTTCAAATCA TGGACCCCAC AGGGAACTCT GCCACTCCTC AAATTTTGGA GTTGAAGTGG 
TCTCATATTG AATGGTCACA AACTGAATAT GAGGTCTGTG AGAATGTGGG CATGTTGTCC 
TTAGAAGTTA CCAGAAGGGG ATATTCCATG GACTCAGCCT TTGTGGGAGT AAAGGTCAAT 
CAAGTGTCAG CTGCAGCTGG AAAAGATTTC ACCGTGACTC CATCCAAACT GATTCAGTTT 
GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA 
GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACGAAG 
ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC ATTGCCATCC TTCATACTCC 
TCCAACCAAA GCAAGCCCAA CACATGGGAG AAGGGCATTT GGCGCCCGTT GCATCCAGGG 
TCTCCCTCGC CCACCACTTC TGGTTCCCTT CATCTGGAAA GAAGACCCCC TCCATCTTCC 
AAGCAGCTGG TGACCACCAG GGGAGACTCC CTACAGGGCT TTGACTCCAC AGCTCTTTCT 
CAGAGGAAGC TGAGGACCCA CTGGAACGGC AAAGCAGTTC ATCCATCTTC TGTTTATAGA 
AACAGAACAG ACATCATCTA TAATTATCAT GGGATAGCTT CCTTGAAACT GGAAGATGAC 
AGTGCCCCAC CTCCCCAAAG GAAGGCCAAA GTATCCATTA TTAGTCAGCC ACAAAAGACA 
ATGAAAGTGG CAGAGCTGCC TGCAGTAGAT AAGGTGGAAT CCACAACTGA CTCTCACTTC 
CCCAGACAGG ACCAGTTGCC CTTATTTCCA AAGAATTGCA CAATGGAATT AAAGGGACTC 
TTCCATTTTG AAGAAAGCAC CCAAAAGCTG TATGGATGCA ATGGGATTGC CTGGAAATCC 
TGGAGCCCCC AAACCAAGGT GGGAAACAGA CACCTGGGAA ATCTTGTAAC TGTATTGTCC 
AGGCAGCACA TGCAGTGGCT CTGGGACATC AGTGGAAGAA AGCCCTTTTG GATAGGTTTG 
AATGACCAAG CACACGCTGG CCACTGGGAA TGGATTGGAG GCGAACCTGT CACCTTCACC 
AACTGGAGGA GAGAGCCCCC TCAATACTCA AAGCCTGGAA AGAACTGTGT TTTGGTTCAA 
AGAGGAGGGA AATGGCAAAC AAAGAACTGT ATGAAGGGCA AACCTCAAAA TTTCATGTGC 
TCCAGGAAAC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Frem1 ( XM_021728214.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021583889.1 Ictidomys tridecemlineatus FRAS1 related extracellular matrix 1 (Frem1), mRNA.

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CloneID	OIc79342
Clone ID Related Accession (Same CDS sequence)	XM_021728214.1
Accession Version	XM_021728214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	FRAS1-related extracellular matrix protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936539.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCAGAA GCGAGTGGGC CCCTGTCAGC ATGAGCTCTC TAGGTCCGGG TGGCCTCAGT 
GTCCTCCTGC TTCTGCTGCT CCTGGACCGG GCCAGCCCTA CCTTCATCAG CCTGAACCAT 
GGGATGAGGG TGATGAAGGG CAGCTCTGCC TTCCTGTCGG AAGATGACCT CAAGTTTGCC 
ATCCCCAAAG AGAAAGATGC CTGCAAAGTG GAAGTTGTGA TGAATGAGCC GATAACCCAG 
AGGGCAGGGA AACTCATGCC ACAGGTCTTT GACTGCCATT TCCTTCCCAA TGAAGTCAGA 
TATGTTCACA ATGGTTGCCC GATTCTTGAT GAAGACACAG TGAAGCTTCG ACTTTACAGA 
TTCACTGAGA CAGACACCTT CACTGAAACC TTTACCCTGC GGGTCTATCT CATGGAGCCA 
GACTGCAATA TTATCCGCAT GAGTAGCAAT GTCCTGGAGG TGTCTGAATT CTACGGCCTG 
TCCCGAGCCA TTGATAAGAA CCTGCTCAGA TTCGACTATG AGAGGATGGT GGGCCTGGAG 
TGTACCGTCA GACTGGATCC TTTGAGAACT AGGCTGCCAG CTCACGGCCA GATGGTTGTG 
GTGGAGCCTC GCCCAGAAGG ACCCCGTGGA GATCAGCCAC ACAGTTTTTT CCCTGAGTCC 
CAACTGGGAA TGGAACTGAA GTGTCCAGGT GGAAGCTGTA CCCTGGAATT GAAGAAAATA 
GCAAGTCTCC AAGTGAGCTG TGAGGAGTTC CTGCTGATGG GGCTTCGTTA TCAACACCTG 
CAACCCCCTT CTCCCAACAT TGACTATATC TCCATCCAAC TGGACCTCAC AGACAGCAGG 
AGTCAAATCG TGTACAAGTC AGAGAGTGCG TGGTTGCCTG TCTACATCAG AGCTGGCATT 
CCGAACCAGA TTCCAAGGGC TGCGTTCATG GCCATGTTCA TTCTGGAAGT GGATCAGTTC 
ATCCTGACCT CTTTGACTAC GTCAGTTCTG GACTGTGAAG AGGATGAGAC CCCCAAACCC 
TTGCTGGTGT TCAACATTAC AAAAGCCCCA CTCCAGGGCT ACATGACACA CCTGTTGGAT 
CACACCAGAC CCGTCTCCTC ATTCACCTGG AAGGATCTCA GCGACATGCA GATTGCCTAT 
CAGCCTCCTA ACAGCAGCCA TTCTGAGAGG AGAAATTATG AGGTGGAGTT GGAGGTATAT 
GACTTTTTCT TTGGAAGGAG TGCACCTGTC ACAGTCCATA TCTCCATCAG AACAGCAGAC 
ACCAACGCCC CCCGGGTGTC CTGGAATACA GGTCTGAATC TCCTGGAGGG ACAGTCTCGG 
GCTATCACTT GGGAACAGTT TCAGATTGTT GACAATGATG ACATTGGTGC CGTCCAGTTG 
GTCACTGTTG ATGGCCTGCA TCACGGACAG CTTACAGTGA GAGGGGGGAA AGGATTTCTC 
TTCACGGTGG CTGACCTTCA GACTGGAGTT GTTCGCTATC ACCATGACGA TAGCGACTCC 
ACTAAAGACT TTGTGGTCTT CAGGATCTTC GATGGCCATC ACAGCATCCG TCACAAGTTT 
CCCATCAACA TCTTGCCCAA GGATGACAGT CCCCCGTTCC TCATAACCAA TGTTGTGATT 
GAACTGGAGG AAGGACAGAC CATCCTGGTG CAGGGTTCCA TGCTGAGAGC CTCAGACATG 
GACTCCAGTG ACGACTACAT CTTCTTCAAC ATCACAAAAC CCCCGCAGGC TGGAGAGATC 
ATGAAGAAGC CAGGGCCAGG GCTGATAGGC TATCCTGTCT CTGGCTTTCT TCAGAGGGAT 
CTGTTTAATG GGATCATTTA CTATCATCAC TTTGGTGGAG AAATCTTTGA AGATTCTTTC 
GAATTTGTCC TGTGGGACAG CCACGAACCT CCAAATCTCT CAGTGCCACA GGTGGTGACC 
ATCCATATCA CTCCAGTGGA TGACCAGCTT CCAAAAGAGG CTCCTGGGGT TTCTCGGCAT 
TTGGTTGTCA AGGAAACAGA GGTGGCCTAC ATAACGAAGA AGCATCTACA TTTCCTAGAT 
ACAGAATCTT ACGATAGGGA GCTGCTCTAC ACAATAACCA CTCCTCCCTG TTTCTCTTTC 
AATGACAGAT ATTTGGATGC TGGGAAATTA TTCATGGTGG ACAGCATACC AAAGCTAACC 
AAAAATCCTA CAGCCCTGGG GCTGAGGTCA TTTACTCAGC ATGCTGTGAA CTATATGAAA 
GTGGCCTACA TGCCACCTAT GCAAGATATA GGCCCTCATC TCCGACATGT CCAGTTTACC 
TTTTCCATCA GTAACCAGCA TGGTGGCACT TTGCACGGGA TCTGCTTTAA TATTACAATT 
CTCCCAGTGG ACAATCAAGT TCCTGAGGTA TCCACCACCC CTCTGAGAGT GGCTGAGGGA 
GGCCAGTGCG TCATCAGCAC AGAGCACATT CTGGTTTCTG ATGTGGATAC CATGTTGGAC 
AATATCTACA TTGCCCTGCA GGGTCTGCCT CTGCATGGAA GGCTGGAGTT GAATGGATTT 
CCTCTAGATG CTGGGGACAC ATTTTCTTGG GGAGACCTTC GTACCTTAAA AGTTAGGTAT 
CAGCATGATG GATCTGAGGT TCTTCAGGAT GACATACGTT TGGAGGTCAC AGATGGCACA 
AATTCAGCAG AATTTGTTTT ACACGTTGAG GTGTTTCCTA TAAATGATGA GCCACCGATC 
CTGAAGGCTG ACCTCATCCC CATGATGCAT TGCTCCGAGG GTGGAGAGGT GGTCATCACC 
CCTGAACACA TTTTTGCTAC TGATATGGAC AGTGATGACT TGAAACTGAT GGTTCTGATT 
GCCCGAGAAC CTCAGCATGG GGTGGTGTGG AAGGCTGGTG TTCAAGTAGA CCAGTTCTCT 
CAGGGAGATA TTATCTCAGG GGCTGTGACA TACAGACACA CAGGTGGTGA GATTGGCCTG 
GTGCCTTGTT TCGATACCAT TACTTTGGTG ATTTCTGATG GAGAAGCTGG CCCTTTTGTG 
AATGGCTGTT GTTACAGTGG ACCTAATCCA TCTGTTCCTC TCCATGAGTC CTTTCCAGTG 
TATGATCTCA ACATCACAGT ACATCCAGTA GACAATCAGC CACCTTCAAT TGCAATAGGT 
ACCTTGTTCC TTGTAGATGA GGGGTTCTCA GCAGCCCTTA CCATTAACCA TTTGTCTGCC 
AGTGATCCTG ACACTGCTGC AGATGACTTG GAATTTGTTT TGGTTTCTCC TCCCCAATTT 
GGTTACCTTG AAAACACACT TCCTTCTGTG GGTTTTGAGA AAAGCAATAT GGGCATAAGT 
ATAGCTTCCT TTCACTGGAA AGACATGAAG GCTTTGCACA TCAACTATGT GCAATCCAGA 
CACCTGAGGA TGGAACCCAC CACTGACCAG TTTGTGGTGT ACGTCACAGA TGGGAAGCAA 
CGCTCCTTGC AGACTCCATT TTATGTTATA ATCAACCCCA CAAATGATGA AGTTCCTGAC 
TTTGTAGTGC AGAATATTAC TGTGTGTGAG GGTCAGACGA CAGAGCTGGA TTCTTCTGTC 
ATCAGTGCTG CCGACCTGGA CATTCCCAAG GACCTCTTAT TGTTCAGCAT CATTCAGAAA 
CCACGTCATG GTCTCCTCAT CAATAGGATG CTCAGCAAAG ACTCTCCTCA GAATAAGCAG 
CCAGCCAACC CTGACCAGAA GCAGGAACTT GTTCACAGCT TTTCCTTGGA ACTTTTCAAG 
ACTGGAATGA GGTTGACCTA TGTGCATGAT GACTCTGAGA GCTTTGCTGA TGACTTTACA 
ATCCAATTGT CAGATGGGAA ACACAAGATA CTTAAAATCA TTTCAGTAGA GATCACTCCG 
GTTAATGATG AGAAACCAAT GCTAAGCAAG AAGGCTGAAA TCGAAATGAA TATGGGGGAT 
ACCCGTATTA TTTCTAGCGC TGTTCTTTCC GCTGTAGACA AAGACTCACC CAGGGAGAAG 
ATTTACTATT TATTTGAAAG GCTTCCCCAA CATGGACAAC TTCAGCTTAA GATAGGGAGA 
GACTGGGTTT CTCTCTCCCC GGGCATGAAA TGCACCCAGG AAGAAGTGGA TCTGAATTTG 
CTGAGATACA CGCACACCGG GGCAATGGAT TCCCAGAATC GAGATAGCTT CACCTTTTAC 
CTCTGGGATG GTGACAACAG ATCACCTGCA TTTGACTGTC ACATCATCAT CAAGGATATG 
GGAAAAGGTT ATATTGTCCT TCTTGCAAAA CCACTTGTGG TATCTAAAGG CGACAGAGGG 
TTCTTGACGA CCGCCACTCT TCTGGCTGTG GATGGAACAG ACAGGCCTGA GGAACTGCTG 
TATGCCATTA CCTCTCCGCC GCGGTATGGC CAGGTGGAAT ATGTCCGCTA TCCCGGGGTC 
CCCATTACAA GCTTCAGCCA ATTGGACATA GCGGGGCAGA CAGTCTGCTA CATACATAAG 
AGTGGGGCAC CTGTGCCCAG TGACAGCTTC AGATTCATCG TCAGCAATGG ACTGTGGACC 
AAGCACGGGG TGTTTGAGAT CACACTGGAG ACTGTAGACA GAGCCTTGCC TGTGGTCACC 
AGGAACAAGG GGATGAGGCT GGCCGAGGGG GCCATGGGCC TGCTTTCTCC TGACCTCCTC 
CAGCTGACTG ACCCTGACAC TCCTGCCGAA AACCTCACCT TCCTCCTGGT CCAGCTCCCA 
CAGCACGGCC GGCTCTACCT GCGGGGAACT GTACTGCTTC GACACAACTT CACCCAGTGG 
GATGTGGACA GCGGGAATGT GGCCTATCAG CACTCTGGAG GCAGCTCCCA GATTGACCAC 
TTCACTTTCA TGGCCACAGA TAGGACAAAC CAAGGCTTTG TTGTGGATGG GAGGGTGCGA 
GAAGAGCCTG TTCCATTCAC CATTCAGGTG GAGCATTTGG ATAAAATAGC CCCCCGGATC 
ACACTCTTGC GTTCCCCTTC ACAAGTGAGA CTCCTGAAAA ACGGCTGTTA TGGGATTTAC 
ATCACTTCCC GAGTGTTGAA GGCATCGGAC CCTGACACCG AGGACCATCA GATAATCTTT 
AAGATTTTAC GAGGCCCACT ACACGGACAT TTGGAGAATA CAACAACAGG TGAATTTATC 
CATGAGAAAT TTAGCCAAAA GGACTTATAC AGTAAGACCA TTCTTTACAT CATAAACCCA 
TCTTTGGAAG TAAATTCAGA TATCGTGGAA TTTCAAATCA TGGACCCCAC AGGGAACTCT 
GCCACTCCTC AAATTTTGGA GTTGAAGTGG TCTCATATTG AATGGTCACA AACTGAATAT 
GAGGTCTGTG AGAATGTGGG CATGTTGTCC TTAGAAGTTA CCAGAAGGGG ATATTCCATG 
GACTCAGCCT TTGTGGGAGT AAAGGTCAAT CAAGTGTCAG CTGCAGCTGG AAAAGATTTC 
ACCGTGACTC CATCCAAACT GATTCAGTTT GACCCAGGAA TGTCAACTAA GATGTGGAAT 
ATAGCAATTA CCTATGACGG ATTAGAGGAA GATGATGAGG TCTTTGAAGT AATTCTGAAC 
TCCCCTGTGA ATGCAGTTCT TGGCACGAAG ACAAAAGCTG CAGTGAAAAT TTTGGACTCA 
AAAGGAGGAC ATTGCCATCC TTCATACTCC TCCAACCAAA GCAAGCCCAA CACATGGGAG 
AAGGGCATTT GGCGCCCGTT GCATCCAGGG TCTCCCTCGC CCACCACTTC TGGTTCCCTT 
CATCTGGAAA GAAGACCCCC TCCATCTTCC AAGCAGCTGG TGACCACCAG GGGAGACTCC 
CTACAGGGCT TTGACTCCAC AGCTCTTTCT CAGAGGAAGC TGAGGACCCA CTGGAACGGC 
AAAGCAGTTC ATCCATCTTC TGTTTATAGA AACAGAACAG ACATCATCTA TAATTATCAT 
GGGATAGCTT CCTTGAAACT GGAAGATGAC AGTGCCCCAC CTCCCCAAAG GAAGGCCAAA 
GTATCCATTA TTAGTCAGCC ACAAAAGACA ATGAAAGTGG CAGAGCTGCC TGCAGTAGAT 
AAGGTGGAAT CCACAACTGA CTCTCACTTC CCCAGACAGG ACCAGTTGCC CTTATTTCCA 
AAGAATTGCA CAATGGAATT AAAGGGACTC TTCCATTTTG AAGAAAGCAC CCAAAAGCTG 
TATGGATGCA ATGGGATTGC CTGGAAATCC TGGAGCCCCC AAACCAAGGT GGGAAACAGG 
CTGAAACACC TGGGAAATCT TGTAACTGTA TTGTCCAGGC AGCACATGCA GTGGCTCTGG 
GACATCAGTG GAAGAAAGCC CTTTTGGATA GGTTTGAATG ACCAAGCACA CGCTGGCCAC 
TGGGAATGGA TTGGAGGCGA ACCTGTCACC TTCACCAACT GGAGGAGAGA GCCCCCTCAA 
TACTCAAAGC CTGGAAAGAA CTGTGTTTTG GTTCAAAGAG GAGGGAAATG GCAAACAAAG 
AACTGTATGA AGGGCAAACC TCAAAATTTC ATGTGCTCCA GGAAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021583889.1
CDS	491..6961
Translation	MARSEWAPVSMSSLGPGGLSVLLLLLLLDRASPTFISLNHGMRVMKGSSAFLSEDDLKFAIPKEKDACKVEVVMNEPITQRAGKLMPQVFDCHFLPNEVRYVHNGCPILDEDTVKLRLYRFTETDTFTETFTLRVYLMEPDCNIIRMSSNVLEVSEFYGLSRAIDKNLLRFDYERMVGLECTVRLDPLRTRLPAHGQMVVVEPRPEGPRGDQPHSFFPESQLGMELKCPGGSCTLELKKIASLQVSCEEFLLMGLRYQHLQPPSPNIDYISIQLDLTDSRSQIVYKSESAWLPVYIRAGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYMTHLLDHTRPVSSFTWKDLSDMQIAYQPPNSSHSERRNYEVELEVYDFFFGRSAPVTVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTVDGLHHGQLTVRGGKGFLFTVADLQTGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINILPKDDSPPFLITNVVIELEEGQTILVQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPGPGLIGYPVSGFLQRDLFNGIIYYHHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKHLHFLDTESYDRELLYTITTPPCFSFNDRYLDAGKLFMVDSIPKLTKNPTALGLRSFTQHAVNYMKVAYMPPMQDIGPHLRHVQFTFSISNQHGGTLHGICFNITILPVDNQVPEVSTTPLRVAEGGQCVISTEHILVSDVDTMLDNIYIALQGLPLHGRLELNGFPLDAGDTFSWGDLRTLKVRYQHDGSEVLQDDIRLEVTDGTNSAEFVLHVEVFPINDEPPILKADLIPMMHCSEGGEVVITPEHIFATDMDSDDLKLMVLIAREPQHGVVWKAGVQVDQFSQGDIISGAVTYRHTGGEIGLVPCFDTITLVISDGEAGPFVNGCCYSGPNPSVPLHESFPVYDLNITVHPVDNQPPSIAIGTLFLVDEGFSAALTINHLSASDPDTAADDLEFVLVSPPQFGYLENTLPSVGFEKSNMGISIASFHWKDMKALHINYVQSRHLRMEPTTDQFVVYVTDGKQRSLQTPFYVIINPTNDEVPDFVVQNITVCEGQTTELDSSVISAADLDIPKDLLLFSIIQKPRHGLLINRMLSKDSPQNKQPANPDQKQELVHSFSLELFKTGMRLTYVHDDSESFADDFTIQLSDGKHKILKIISVEITPVNDEKPMLSKKAEIEMNMGDTRIISSAVLSAVDKDSPREKIYYLFERLPQHGQLQLKIGRDWVSLSPGMKCTQEEVDLNLLRYTHTGAMDSQNRDSFTFYLWDGDNRSPAFDCHIIIKDMGKGYIVLLAKPLVVSKGDRGFLTTATLLAVDGTDRPEELLYAITSPPRYGQVEYVRYPGVPITSFSQLDIAGQTVCYIHKSGAPVPSDSFRFIVSNGLWTKHGVFEITLETVDRALPVVTRNKGMRLAEGAMGLLSPDLLQLTDPDTPAENLTFLLVQLPQHGRLYLRGTVLLRHNFTQWDVDSGNVAYQHSGGSSQIDHFTFMATDRTNQGFVVDGRVREEPVPFTIQVEHLDKIAPRITLLRSPSQVRLLKNGCYGIYITSRVLKASDPDTEDHQIIFKILRGPLHGHLENTTTGEFIHEKFSQKDLYSKTILYIINPSLEVNSDIVEFQIMDPTGNSATPQILELKWSHIEWSQTEYEVCENVGMLSLEVTRRGYSMDSAFVGVKVNQVSAAAGKDFTVTPSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGHCHPSYSSNQSKPNTWEKGIWRPLHPGSPSPTTSGSLHLERRPPPSSKQLVTTRGDSLQGFDSTALSQRKLRTHWNGKAVHPSSVYRNRTDIIYNYHGIASLKLEDDSAPPPQRKAKVSIISQPQKTMKVAELPAVDKVESTTDSHFPRQDQLPLFPKNCTMELKGLFHFEESTQKLYGCNGIAWKSWSPQTKVGNRLKHLGNLVTVLSRQHMQWLWDISGRKPFWIGLNDQAHAGHWEWIGGEPVTFTNWRREPPQYSKPGKNCVLVQRGGKWQTKNCMKGKPQNFMCSRKL

Target ORF information:

RefSeq Version	XM_021728214.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus FRAS1 related extracellular matrix 1 (Frem1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021728214.1

ORF Insert Sequence:

ATGGCCAGAA GCGAGTGGGC CCCTGTCAGC ATGAGCTCTC TAGGTCCGGG TGGCCTCAGT 
GTCCTCCTGC TTCTGCTGCT CCTGGACCGG GCCAGCCCTA CCTTCATCAG CCTGAACCAT 
GGGATGAGGG TGATGAAGGG CAGCTCTGCC TTCCTGTCGG AAGATGACCT CAAGTTTGCC 
ATCCCCAAAG AGAAAGATGC CTGCAAAGTG GAAGTTGTGA TGAATGAGCC GATAACCCAG 
AGGGCAGGGA AACTCATGCC ACAGGTCTTT GACTGCCATT TCCTTCCCAA TGAAGTCAGA 
TATGTTCACA ATGGTTGCCC GATTCTTGAT GAAGACACAG TGAAGCTTCG ACTTTACAGA 
TTCACTGAGA CAGACACCTT CACTGAAACC TTTACCCTGC GGGTCTATCT CATGGAGCCA 
GACTGCAATA TTATCCGCAT GAGTAGCAAT GTCCTGGAGG TGTCTGAATT CTACGGCCTG 
TCCCGAGCCA TTGATAAGAA CCTGCTCAGA TTCGACTATG AGAGGATGGT GGGCCTGGAG 
TGTACCGTCA GACTGGATCC TTTGAGAACT AGGCTGCCAG CTCACGGCCA GATGGTTGTG 
GTGGAGCCTC GCCCAGAAGG ACCCCGTGGA GATCAGCCAC ACAGTTTTTT CCCTGAGTCC 
CAACTGGGAA TGGAACTGAA GTGTCCAGGT GGAAGCTGTA CCCTGGAATT GAAGAAAATA 
GCAAGTCTCC AAGTGAGCTG TGAGGAGTTC CTGCTGATGG GGCTTCGTTA TCAACACCTG 
CAACCCCCTT CTCCCAACAT TGACTATATC TCCATCCAAC TGGACCTCAC AGACAGCAGG 
AGTCAAATCG TGTACAAGTC AGAGAGTGCG TGGTTGCCTG TCTACATCAG AGCTGGCATT 
CCGAACCAGA TTCCAAGGGC TGCGTTCATG GCCATGTTCA TTCTGGAAGT GGATCAGTTC 
ATCCTGACCT CTTTGACTAC GTCAGTTCTG GACTGTGAAG AGGATGAGAC CCCCAAACCC 
TTGCTGGTGT TCAACATTAC AAAAGCCCCA CTCCAGGGCT ACATGACACA CCTGTTGGAT 
CACACCAGAC CCGTCTCCTC ATTCACCTGG AAGGATCTCA GCGACATGCA GATTGCCTAT 
CAGCCTCCTA ACAGCAGCCA TTCTGAGAGG AGAAATTATG AGGTGGAGTT GGAGGTATAT 
GACTTTTTCT TTGGAAGGAG TGCACCTGTC ACAGTCCATA TCTCCATCAG AACAGCAGAC 
ACCAACGCCC CCCGGGTGTC CTGGAATACA GGTCTGAATC TCCTGGAGGG ACAGTCTCGG 
GCTATCACTT GGGAACAGTT TCAGATTGTT GACAATGATG ACATTGGTGC CGTCCAGTTG 
GTCACTGTTG ATGGCCTGCA TCACGGACAG CTTACAGTGA GAGGGGGGAA AGGATTTCTC 
TTCACGGTGG CTGACCTTCA GACTGGAGTT GTTCGCTATC ACCATGACGA TAGCGACTCC 
ACTAAAGACT TTGTGGTCTT CAGGATCTTC GATGGCCATC ACAGCATCCG TCACAAGTTT 
CCCATCAACA TCTTGCCCAA GGATGACAGT CCCCCGTTCC TCATAACCAA TGTTGTGATT 
GAACTGGAGG AAGGACAGAC CATCCTGGTG CAGGGTTCCA TGCTGAGAGC CTCAGACATG 
GACTCCAGTG ACGACTACAT CTTCTTCAAC ATCACAAAAC CCCCGCAGGC TGGAGAGATC 
ATGAAGAAGC CAGGGCCAGG GCTGATAGGC TATCCTGTCT CTGGCTTTCT TCAGAGGGAT 
CTGTTTAATG GGATCATTTA CTATCATCAC TTTGGTGGAG AAATCTTTGA AGATTCTTTC 
GAATTTGTCC TGTGGGACAG CCACGAACCT CCAAATCTCT CAGTGCCACA GGTGGTGACC 
ATCCATATCA CTCCAGTGGA TGACCAGCTT CCAAAAGAGG CTCCTGGGGT TTCTCGGCAT 
TTGGTTGTCA AGGAAACAGA GGTGGCCTAC ATAACGAAGA AGCATCTACA TTTCCTAGAT 
ACAGAATCTT ACGATAGGGA GCTGCTCTAC ACAATAACCA CTCCTCCCTG TTTCTCTTTC 
AATGACAGAT ATTTGGATGC TGGGAAATTA TTCATGGTGG ACAGCATACC AAAGCTAACC 
AAAAATCCTA CAGCCCTGGG GCTGAGGTCA TTTACTCAGC ATGCTGTGAA CTATATGAAA 
GTGGCCTACA TGCCACCTAT GCAAGATATA GGCCCTCATC TCCGACATGT CCAGTTTACC 
TTTTCCATCA GTAACCAGCA TGGTGGCACT TTGCACGGGA TCTGCTTTAA TATTACAATT 
CTCCCAGTGG ACAATCAAGT TCCTGAGGTA TCCACCACCC CTCTGAGAGT GGCTGAGGGA 
GGCCAGTGCG TCATCAGCAC AGAGCACATT CTGGTTTCTG ATGTGGATAC CATGTTGGAC 
AATATCTACA TTGCCCTGCA GGGTCTGCCT CTGCATGGAA GGCTGGAGTT GAATGGATTT 
CCTCTAGATG CTGGGGACAC ATTTTCTTGG GGAGACCTTC GTACCTTAAA AGTTAGGTAT 
CAGCATGATG GATCTGAGGT TCTTCAGGAT GACATACGTT TGGAGGTCAC AGATGGCACA 
AATTCAGCAG AATTTGTTTT ACACGTTGAG GTGTTTCCTA TAAATGATGA GCCACCGATC 
CTGAAGGCTG ACCTCATCCC CATGATGCAT TGCTCCGAGG GTGGAGAGGT GGTCATCACC 
CCTGAACACA TTTTTGCTAC TGATATGGAC AGTGATGACT TGAAACTGAT GGTTCTGATT 
GCCCGAGAAC CTCAGCATGG GGTGGTGTGG AAGGCTGGTG TTCAAGTAGA CCAGTTCTCT 
CAGGGAGATA TTATCTCAGG GGCTGTGACA TACAGACACA CAGGTGGTGA GATTGGCCTG 
GTGCCTTGTT TCGATACCAT TACTTTGGTG ATTTCTGATG GAGAAGCTGG CCCTTTTGTG 
AATGGCTGTT GTTACAGTGG ACCTAATCCA TCTGTTCCTC TCCATGAGTC CTTTCCAGTG 
TATGATCTCA ACATCACAGT ACATCCAGTA GACAATCAGC CACCTTCAAT TGCAATAGGT 
ACCTTGTTCC TTGTAGATGA GGGGTTCTCA GCAGCCCTTA CCATTAACCA TTTGTCTGCC 
AGTGATCCTG ACACTGCTGC AGATGACTTG GAATTTGTTT TGGTTTCTCC TCCCCAATTT 
GGTTACCTTG AAAACACACT TCCTTCTGTG GGTTTTGAGA AAAGCAATAT GGGCATAAGT 
ATAGCTTCCT TTCACTGGAA AGACATGAAG GCTTTGCACA TCAACTATGT GCAATCCAGA 
CACCTGAGGA TGGAACCCAC CACTGACCAG TTTGTGGTGT ACGTCACAGA TGGGAAGCAA 
CGCTCCTTGC AGACTCCATT TTATGTTATA ATCAACCCCA CAAATGATGA AGTTCCTGAC 
TTTGTAGTGC AGAATATTAC TGTGTGTGAG GGTCAGACGA CAGAGCTGGA TTCTTCTGTC 
ATCAGTGCTG CCGACCTGGA CATTCCCAAG GACCTCTTAT TGTTCAGCAT CATTCAGAAA 
CCACGTCATG GTCTCCTCAT CAATAGGATG CTCAGCAAAG ACTCTCCTCA GAATAAGCAG 
CCAGCCAACC CTGACCAGAA GCAGGAACTT GTTCACAGCT TTTCCTTGGA ACTTTTCAAG 
ACTGGAATGA GGTTGACCTA TGTGCATGAT GACTCTGAGA GCTTTGCTGA TGACTTTACA 
ATCCAATTGT CAGATGGGAA ACACAAGATA CTTAAAATCA TTTCAGTAGA GATCACTCCG 
GTTAATGATG AGAAACCAAT GCTAAGCAAG AAGGCTGAAA TCGAAATGAA TATGGGGGAT 
ACCCGTATTA TTTCTAGCGC TGTTCTTTCC GCTGTAGACA AAGACTCACC CAGGGAGAAG 
ATTTACTATT TATTTGAAAG GCTTCCCCAA CATGGACAAC TTCAGCTTAA GATAGGGAGA 
GACTGGGTTT CTCTCTCCCC GGGCATGAAA TGCACCCAGG AAGAAGTGGA TCTGAATTTG 
CTGAGATACA CGCACACCGG GGCAATGGAT TCCCAGAATC GAGATAGCTT CACCTTTTAC 
CTCTGGGATG GTGACAACAG ATCACCTGCA TTTGACTGTC ACATCATCAT CAAGGATATG 
GGAAAAGGTT ATATTGTCCT TCTTGCAAAA CCACTTGTGG TATCTAAAGG CGACAGAGGG 
TTCTTGACGA CCGCCACTCT TCTGGCTGTG GATGGAACAG ACAGGCCTGA GGAACTGCTG 
TATGCCATTA CCTCTCCGCC GCGGTATGGC CAGGTGGAAT ATGTCCGCTA TCCCGGGGTC 
CCCATTACAA GCTTCAGCCA ATTGGACATA GCGGGGCAGA CAGTCTGCTA CATACATAAG 
AGTGGGGCAC CTGTGCCCAG TGACAGCTTC AGATTCATCG TCAGCAATGG ACTGTGGACC 
AAGCACGGGG TGTTTGAGAT CACACTGGAG ACTGTAGACA GAGCCTTGCC TGTGGTCACC 
AGGAACAAGG GGATGAGGCT GGCCGAGGGG GCCATGGGCC TGCTTTCTCC TGACCTCCTC 
CAGCTGACTG ACCCTGACAC TCCTGCCGAA AACCTCACCT TCCTCCTGGT CCAGCTCCCA 
CAGCACGGCC GGCTCTACCT GCGGGGAACT GTACTGCTTC GACACAACTT CACCCAGTGG 
GATGTGGACA GCGGGAATGT GGCCTATCAG CACTCTGGAG GCAGCTCCCA GATTGACCAC 
TTCACTTTCA TGGCCACAGA TAGGACAAAC CAAGGCTTTG TTGTGGATGG GAGGGTGCGA 
GAAGAGCCTG TTCCATTCAC CATTCAGGTG GAGCATTTGG ATAAAATAGC CCCCCGGATC 
ACACTCTTGC GTTCCCCTTC ACAAGTGAGA CTCCTGAAAA ACGGCTGTTA TGGGATTTAC 
ATCACTTCCC GAGTGTTGAA GGCATCGGAC CCTGACACCG AGGACCATCA GATAATCTTT 
AAGATTTTAC GAGGCCCACT ACACGGACAT TTGGAGAATA CAACAACAGG TGAATTTATC 
CATGAGAAAT TTAGCCAAAA GGACTTATAC AGTAAGACCA TTCTTTACAT CATAAACCCA 
TCTTTGGAAG TAAATTCAGA TATCGTGGAA TTTCAAATCA TGGACCCCAC AGGGAACTCT 
GCCACTCCTC AAATTTTGGA GTTGAAGTGG TCTCATATTG AATGGTCACA AACTGAATAT 
GAGGTCTGTG AGAATGTGGG CATGTTGTCC TTAGAAGTTA CCAGAAGGGG ATATTCCATG 
GACTCAGCCT TTGTGGGAGT AAAGGTCAAT CAAGTGTCAG CTGCAGCTGG AAAAGATTTC 
ACCGTGACTC CATCCAAACT GATTCAGTTT GACCCAGGAA TGTCAACTAA GATGTGGAAT 
ATAGCAATTA CCTATGACGG ATTAGAGGAA GATGATGAGG TCTTTGAAGT AATTCTGAAC 
TCCCCTGTGA ATGCAGTTCT TGGCACGAAG ACAAAAGCTG CAGTGAAAAT TTTGGACTCA 
AAAGGAGGAC ATTGCCATCC TTCATACTCC TCCAACCAAA GCAAGCCCAA CACATGGGAG 
AAGGGCATTT GGCGCCCGTT GCATCCAGGG TCTCCCTCGC CCACCACTTC TGGTTCCCTT 
CATCTGGAAA GAAGACCCCC TCCATCTTCC AAGCAGCTGG TGACCACCAG GGGAGACTCC 
CTACAGGGCT TTGACTCCAC AGCTCTTTCT CAGAGGAAGC TGAGGACCCA CTGGAACGGC 
AAAGCAGTTC ATCCATCTTC TGTTTATAGA AACAGAACAG ACATCATCTA TAATTATCAT 
GGGATAGCTT CCTTGAAACT GGAAGATGAC AGTGCCCCAC CTCCCCAAAG GAAGGCCAAA 
GTATCCATTA TTAGTCAGCC ACAAAAGACA ATGAAAGTGG CAGAGCTGCC TGCAGTAGAT 
AAGGTGGAAT CCACAACTGA CTCTCACTTC CCCAGACAGG ACCAGTTGCC CTTATTTCCA 
AAGAATTGCA CAATGGAATT AAAGGGACTC TTCCATTTTG AAGAAAGCAC CCAAAAGCTG 
TATGGATGCA ATGGGATTGC CTGGAAATCC TGGAGCCCCC AAACCAAGGT GGGAAACAGG 
CTGAAACACC TGGGAAATCT TGTAACTGTA TTGTCCAGGC AGCACATGCA GTGGCTCTGG 
GACATCAGTG GAAGAAAGCC CTTTTGGATA GGTTTGAATG ACCAAGCACA CGCTGGCCAC 
TGGGAATGGA TTGGAGGCGA ACCTGTCACC TTCACCAACT GGAGGAGAGA GCCCCCTCAA 
TACTCAAAGC CTGGAAAGAA CTGTGTTTTG GTTCAAAGAG GAGGGAAATG GCAAACAAAG 
AACTGTATGA AGGGCAAACC TCAAAATTTC ATGTGCTCCA GGAAACTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Frem1 cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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