Agrn gene cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

mRNA	Protein	Name
XM_005339270.2	XP_005339327.1	agrin isoform X7
XM_021735920.1	XP_021591595.1	agrin isoform X6
XM_021735916.1	XP_021591591.1	agrin isoform X2
XM_005339270.1	XP_005339327.1	agrin isoform X1
XM_021735915.1	XP_021591590.1	agrin isoform X1
XM_021735917.1	XP_021591592.1	agrin isoform X3
XM_021735921.1	XP_021591596.1	agrin isoform X8
XM_005339271.1	XP_005339328.1	agrin isoform X2
XM_021735918.1	XP_021591593.1	agrin isoform X4
XM_005339271.2	XP_005339328.1	agrin isoform X10
XM_021735922.1	XP_021591597.1	agrin isoform X9
XM_021735919.1	XP_021591594.1	agrin isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIc70063	XM_005339270.2 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc70064	XM_005339271.2 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84642	XM_021735915.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84643	XM_021735916.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84644	XM_021735917.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84645	XM_021735918.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84646	XM_021735919.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84647	XM_021735920.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84648	XM_021735921.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc84649	XM_021735922.1 Latest version!	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OIc70063	XM_005339270.1	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OIc70064	XM_005339271.1	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

Agrn ( XM_005339270.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005339327.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OIc70063
Clone ID Related Accession (Same CDS sequence)	XM_005339270.2 , XM_005339270.1
Accession Version	XM_005339270.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005339270.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GAAATCCCGC AAGGTGCCGC ATACCATGCT CAATTTGAAG 
GAGCCACTCT ACATCGGGGG TGCCCCCGAC TTCAGCAAGC TGGCCCGGGC AGCTGCAGCA 
TCCTCTGGCT TCGACGGTGC CGTTCAGCTG GTCTCCTTGG GAGGCCGCCA GCTGTTGGCC 
CAGGAGCACG TGGTGCAGGC GGTGGACGTC TCATCCTTTA CAGACCACCC TTGTACCCAG 
GCCTCAGGCC ACCCCTGCCT CCATGGCGCC TTGTGTCTCC CAAAGGAAGC CACCTACACA 
TGCCTGTGCC CCATGGGCTT CTCGGGGCCA CACTGCGAGA AGGGGCTGGT TGAGAAGTCA 
GCAGGGGACC TCGAGGCTCT GGCCTTCGAT GGGAGGACCT ACATCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAAGC CCTGCAGACC AACCACTTCG AGCTCAGCTT GCGCACTGAA 
GCTACGCAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACCG AGCGTGCGGA CTACATGGCG 
CTAGCCATCG TGGATGGACA CCTTCAGCTG GCCTATGACC TGGGCTCTCA GCCTGTGGTG 
CTGCGCTCCA CCGTGAGGGT CAACACCAAC CGCTGGTTGC GAGTCAGGGC TCACAGGGAG 
CAGAGAGAAG GTTCCCTCCA GGTGGGCAAT GAGGCCCCCG TGACTGGCTC CTCCCCACTG 
GGTGCCACAC AGCTGGACAC AGATGGAACT CTCTGGCTCG GGGGCCTGCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCAAA GGCCTACGGA ACAGGTTTTG TGGGCTGCCT CCGGGATGTG 
GTGGTAGGCC AGCGTCCACT GCACCTGCTG GAAGATGCCC TCACCAAGCC AGAGCTGCGG 
CCCTGCCCAG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005339327.1
CDS	1..5838
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_005339270.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005339270.2

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GAAATCCCGC AAGGTGCCGC ATACCATGCT CAATTTGAAG 
GAGCCACTCT ACATCGGGGG TGCCCCCGAC TTCAGCAAGC TGGCCCGGGC AGCTGCAGCA 
TCCTCTGGCT TCGACGGTGC CGTTCAGCTG GTCTCCTTGG GAGGCCGCCA GCTGTTGGCC 
CAGGAGCACG TGGTGCAGGC GGTGGACGTC TCATCCTTTA CAGACCACCC TTGTACCCAG 
GCCTCAGGCC ACCCCTGCCT CCATGGCGCC TTGTGTCTCC CAAAGGAAGC CACCTACACA 
TGCCTGTGCC CCATGGGCTT CTCGGGGCCA CACTGCGAGA AGGGGCTGGT TGAGAAGTCA 
GCAGGGGACC TCGAGGCTCT GGCCTTCGAT GGGAGGACCT ACATCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAAGC CCTGCAGACC AACCACTTCG AGCTCAGCTT GCGCACTGAA 
GCTACGCAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACCG AGCGTGCGGA CTACATGGCG 
CTAGCCATCG TGGATGGACA CCTTCAGCTG GCCTATGACC TGGGCTCTCA GCCTGTGGTG 
CTGCGCTCCA CCGTGAGGGT CAACACCAAC CGCTGGTTGC GAGTCAGGGC TCACAGGGAG 
CAGAGAGAAG GTTCCCTCCA GGTGGGCAAT GAGGCCCCCG TGACTGGCTC CTCCCCACTG 
GGTGCCACAC AGCTGGACAC AGATGGAACT CTCTGGCTCG GGGGCCTGCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCAAA GGCCTACGGA ACAGGTTTTG TGGGCTGCCT CCGGGATGTG 
GTGGTAGGCC AGCGTCCACT GCACCTGCTG GAAGATGCCC TCACCAAGCC AGAGCTGCGG 
CCCTGCCCAG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_005339270.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005339327.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X1, mRNA.

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CloneID	OIc70063
Clone ID Related Accession (Same CDS sequence)	XM_005339270.2 , XM_005339270.1
Accession Version	XM_005339270.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	agrin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GAAATCCCGC AAGGTGCCGC ATACCATGCT CAATTTGAAG 
GAGCCACTCT ACATCGGGGG TGCCCCCGAC TTCAGCAAGC TGGCCCGGGC AGCTGCAGCA 
TCCTCTGGCT TCGACGGTGC CGTTCAGCTG GTCTCCTTGG GAGGCCGCCA GCTGTTGGCC 
CAGGAGCACG TGGTGCAGGC GGTGGACGTC TCATCCTTTA CAGACCACCC TTGTACCCAG 
GCCTCAGGCC ACCCCTGCCT CCATGGCGCC TTGTGTCTCC CAAAGGAAGC CACCTACACA 
TGCCTGTGCC CCATGGGCTT CTCGGGGCCA CACTGCGAGA AGGGGCTGGT TGAGAAGTCA 
GCAGGGGACC TCGAGGCTCT GGCCTTCGAT GGGAGGACCT ACATCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAAGC CCTGCAGACC AACCACTTCG AGCTCAGCTT GCGCACTGAA 
GCTACGCAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACCG AGCGTGCGGA CTACATGGCG 
CTAGCCATCG TGGATGGACA CCTTCAGCTG GCCTATGACC TGGGCTCTCA GCCTGTGGTG 
CTGCGCTCCA CCGTGAGGGT CAACACCAAC CGCTGGTTGC GAGTCAGGGC TCACAGGGAG 
CAGAGAGAAG GTTCCCTCCA GGTGGGCAAT GAGGCCCCCG TGACTGGCTC CTCCCCACTG 
GGTGCCACAC AGCTGGACAC AGATGGAACT CTCTGGCTCG GGGGCCTGCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCAAA GGCCTACGGA ACAGGTTTTG TGGGCTGCCT CCGGGATGTG 
GTGGTAGGCC AGCGTCCACT GCACCTGCTG GAAGATGCCC TCACCAAGCC AGAGCTGCGG 
CCCTGCCCAG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005339327.1
CDS	1..5838
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_005339270.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005339270.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GAAATCCCGC AAGGTGCCGC ATACCATGCT CAATTTGAAG 
GAGCCACTCT ACATCGGGGG TGCCCCCGAC TTCAGCAAGC TGGCCCGGGC AGCTGCAGCA 
TCCTCTGGCT TCGACGGTGC CGTTCAGCTG GTCTCCTTGG GAGGCCGCCA GCTGTTGGCC 
CAGGAGCACG TGGTGCAGGC GGTGGACGTC TCATCCTTTA CAGACCACCC TTGTACCCAG 
GCCTCAGGCC ACCCCTGCCT CCATGGCGCC TTGTGTCTCC CAAAGGAAGC CACCTACACA 
TGCCTGTGCC CCATGGGCTT CTCGGGGCCA CACTGCGAGA AGGGGCTGGT TGAGAAGTCA 
GCAGGGGACC TCGAGGCTCT GGCCTTCGAT GGGAGGACCT ACATCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAAGC CCTGCAGACC AACCACTTCG AGCTCAGCTT GCGCACTGAA 
GCTACGCAGG GGCTGGTGCT GTGGAGTGGC AAGGCCACCG AGCGTGCGGA CTACATGGCG 
CTAGCCATCG TGGATGGACA CCTTCAGCTG GCCTATGACC TGGGCTCTCA GCCTGTGGTG 
CTGCGCTCCA CCGTGAGGGT CAACACCAAC CGCTGGTTGC GAGTCAGGGC TCACAGGGAG 
CAGAGAGAAG GTTCCCTCCA GGTGGGCAAT GAGGCCCCCG TGACTGGCTC CTCCCCACTG 
GGTGCCACAC AGCTGGACAC AGATGGAACT CTCTGGCTCG GGGGCCTGCA GAAGCTGCCT 
GTGGGCCAGG CCCTGCCAAA GGCCTACGGA ACAGGTTTTG TGGGCTGCCT CCGGGATGTG 
GTGGTAGGCC AGCGTCCACT GCACCTGCTG GAAGATGCCC TCACCAAGCC AGAGCTGCGG 
CCCTGCCCAG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_005339271.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005339328.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X2, mRNA.

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CloneID	OIc70064
Clone ID Related Accession (Same CDS sequence)	XM_005339271.1 , XM_005339271.2
Accession Version	XM_005339271.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1439222400000
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Product	agrin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAACT CTAGCCTCAT GCGGATCACC CTTCGCAACC TGGAGGAGGT GGAGTTTTGT 
GTAGAAGACA AACCTGGGAC CCACTTCACA CCAGCACCTC CCATGCCTCC TGACGTCTGC 
AGGGGAATGC TGTGTGGCTT TGGCGCGGTC TGTGAGCCCA GTGCAGAGGA TCCAGGCCGG 
GCCTCCTGCG TGTGCAAGAA GAGCGCCTGT CCTGCCGTCG TGGCGCCAGT GTGCGGCTCA 
GATGCCTCCA CCTACAGCAA CGAGTGCGAG CTGCAGCGTG CACAGTGCCG CCTGCAGCGG 
CGAATCCGCC TGCTCCGCCG CGGACCCTGT GGTTCCCGGG ACCCCTGCTC CAATGTGACC 
TGCAGCTTCG GCAGCACCTG TGCCCCCTCC GCCGATGGGC AGACCGCCTC CTGCCTGTGT 
CCCACGACCT GCCGCGGGGC CCCGGAGGGC ACCGTGTGTG GCAGTGATGG TATGGACTAC 
CCCGGTGAGT GCCAGCTGCT GCGCCGGGCA TGCGCACGCC AGGAGAACAT CTTTAGGAAG 
TTCGACGGCC CCTGTGACCC CTGCCAGGGA GCCCATGCTG ACCCGAGCCG CGTCTGCCGT 
GTGAACCCGC GCACTCGCCG TCCAGAGATG CTCCTGCGCC CAGAGAGCTG CCCTCCCCGG 
CATTTGCCTG TGTGTGGGGA TGATGGGGTC ACCTATGCAA ATGACTGCGT CATGGGCCGC 
TCAGGGGCTG TCCGCGGTCT GCTCCTCCAG AAAGTGCGCT CTGGCCAGTG CCAGCCTCGA 
GACCAGTGCT CCGGGACCTG CCAGTTCAGT GCCGTTTGCC TGTCCCGCCG TGGCCGACCC 
CGCTGCTCCT GCGACCGTGT CACCTGTGAT GGGGCCTACA GACCGGTGTG TGCCCAGGAT 
GGGCACACGT ATGACAACGA CTGTTGGCGC CAGCAGGCTG AGTGCCGGCA GCAGCGGGCC 
ATCCCTCCCA GGCACCAGGG CCCGTGTGAC CAGGCTCCGT CCCCGTGCCG GGGGGTGCAG 
TGCGCATTCG GGGCGACATG CGCCGTGAAG AACGGGGAGG CTGCGTGCGA GTGCCAGCAG 
GCGTGTGCAG GCGCCTACGA CCCCGTGTGC GGCAGTGACG GCGTCACCTA TGGCAGCACC 
TGCGAGCTGG AGGCCATGGC CTGTGCCCTT GGGCGGGATA TCCGGGTGGC CCGCAGAGGA 
CCCTGTGACC GATGCGGGCA GTGCCGTTTT GGAGCCCTGT GCGAGGCCGA GACCGGGCGT 
TGTGTGTGCC CCTCTGAGTG CGTGGCCTCA GCCCAGCCCG TGTGCGGTTC TGATGGGCAC 
ACGTATGCCA GCGAGTGTGA GCTGCATGTC CACGCCTGCA CACACCAGAT AAGCCTGCAT 
GTGGCATCAG CTGGACACTG TCAGACCTGT GGAGACACAG TGTGTGCCTT TGGGGCTGTG 
TGCTCAGCCG GGCAGTGTGT GTGTCCTCGT TGTGAGCGCC CCCCGCCTGG CCCCGTGTGT 
GGCAGCGACG GTGTCACCTA CCGCAGTGCG TGTGAGCTAC GGGAGGCTGC CTGCCAGCAG 
CAGATACAGC TTGAGGAAGC CCGGGCAGGG CCCTGTGAGC AGGCTGAGTG TGGCTCAGGA 
GGCTCCGGCT CTGGGGAGGA CAGCGAGTGT GAGCAGGAAC TCTGCCGGCA GCGAGGCGGG 
GTGTGGGATG AGGACTCAGA GGATGGACCC TGCGTCTGCG ACTTCAGCTG CCAGGGTGTT 
CTCAGGAGCC TGGTGTGCGG CTCAGATGGG GTCACCTATG GCAGTGAGTG TGACCTGAAG 
AGGGCCAGGT GCGAGTCTCA AGGAGAGTTG TACGTCGCAG CTCAGGGACC CTGCCGAGGC 
CTCACCTTGG CCCCACTGCT GCCTGCAGCC CCCGCTCAAT GTGCCCAGAC CCCCTACGGA 
TGCTGCCAGG ATAATGTCAC TGCTGCCCGG GGTGTGGGCC TGGCCGGTTG CCCCAGCACC 
TGCCAGTGCA ACCCTCACGG CTCCTACGGA GGCACCTGTG ACCCAGCCAC AGGCCAGTGC 
TCTTGCCGGC CAGGTGTGGG CGGCCTCAAG TGTGACCGAT GTGAGCCTGG TTTCTGGAAC 
TTCCGTGGCA TTGTCACTGA CGGCCGGAGC GGCTGTACTC CCTGCAGCTG TGACCCCCGG 
GGTGCCGTGC GGGATGACTG TGAGCAGATG ACTGGGCTGT GCTCCTGTAA GCCGGGCGTC 
GCTGGTCCCA AGTGCGGGCA GTGTCCTGAT GGCCACACCC TGGGCCCCAC CGGCTGTGAG 
GCAGATCCTT CAGCACCGGT GACCTGTGCG GAGATGCACT GTGAGTTTGG CGCTTCCTGC 
GTGGAAGAGG CAGGTTCTGC CCACTGCGTC TGCCCAGCCC TCACCTGCCC AGAGGCCAGT 
GCCACCAAGG TCTGTGGATC AGATGGCATC ACCTACGGCA GCGAGTGCCA ACTGAAGACC 
ATCGCCTGCC GCCAGGGCTT GGACATCTTC ACCCAGAGCC TCGGCCCGTG CCAGGAGGCT 
GTGGTTCCTG GCGCTCACCC GACCTCCAGG GCCACGACGG CCCCGGGGCG CTTGCCGAGC 
GAGCCTCTGC TGCCTCCACC CAGTGCCCTC CCCCTGGCTC CCAGCAGTAC CCCCCAGAGC 
CGGCCCACCC CTCCACCCAC CTTACGACCT CGGACCACAG CCAGCCTCCC CAGGAGCACA 
GCTCGGCCGG CGCTGACCAT GCCTCCCATA GAGACCTCCA CTGCAACCAG TCTCTCCGCC 
TCTGCCTTTG GAGAGTCTGG CAGCACTGAT GGGAGTGGCG ATGAGGAACT GAGTGGGGAC 
CAGGAGGCCA GTGGGGGTGG TTCTGGGGGA CTGGAACTCT CCATGGACAG CAGCGTGGTG 
ACACAGGGAC CACCCATGGA GAGAGCCTCC TGCTACAACT CACCTTTGGG CTGCTGTTCG 
GATGGCCAGA CACCTTCGCT GGACGCAGAG GGCTCCAACT GTCCTGCCAC CAAGGTGTAC 
CAGGGCGTGC TGGAGCTGGA GGGGGTCGAG GGGCAAGAGC TGTTCTACAC GCCCGAAATG 
GCCGACCCCA AGTCAGAGCT GTTTGGGGAG ACGGCCAGGA GCATCGAGAG CACTCTGGAC 
GCCCTTTTCC GGAACTCAGA CGTCAAAAAG GACTTCCGGA GTGTCCACCT GCGGGACCTG 
GGGCCTGGCA GATCAGTCCG TGCCACTGTG GATGTGCACT TTGACCCCAC CACAGCCTTC 
AGGGCACCTG ACGTGGGCCG GGCCCTGCTG CGCCAGATCC AGGCCTCCAG GCGCCGGGCC 
CTGGCGGTGC GGAGGCCTCT GCAGGAGCAC GTGCGGTTCA TGGACTTCGA CTGGTTTCCT 
GCGTTCCTCA CGGGAGCCAC CACAGGAGCC ACCGCTGCAG TGGCCACGGC CAGAGCTACC 
ACTGTGGGCC CACCACCTTC CTCCGTGACC CCTCGGGTCC CCCCCAGTCA CACCAGCAGG 
CCCACTGGAA GGACCACAGC ACCCACACAC ACACACCGGC CCCCCACCAC AGCTCCCGGC 
CATGTGGCTG GACGCTGGCC TTTGCTCCCC AATCCCAAGC AGCACCCCAG GTCCTGTGAT 
TCTCAGCCCT GCCTGCATGG GGGGACCTGC CAGGACCAGG ACTCTGGCAG GGGCTTCACC 
TGCAGCTGCC CGGCAGGCCG GGGAGGCACC TTCTGTGAGG ACGTGCTGCG CCCCTCTGTC 
TCAGTTCCTG CGTTTGGGGG TGCCTCCTTT CTGGCCTTCC CCACCCTCCG GGCATATCAC 
ACGCTGCGCC TGGCGCTGGA ATTCCGATCC CTGGAGCCTC AGGGACTGCT GCTGTACAAC 
GGCAACGCCC GTGGCAAGGA CTTCCTGGCC CTGGCGCTGC TCGGTGGGCG CGTGCAGCTC 
AGGTTTGACA CAGGTTCTGG ACCAGCAATA CTGACCAGCT CGGTGCCTGT GGAACCAGGG 
CGGTGGCATC GCCTAGAACT CTCACGGCAC TGGCGCCAAG GCACACTGTC TGTGGATGGC 
GAGACCCCAG TTCTGGGCGA GAGCCCCAGT GGCACGGATG GCCTCAACCT AGATACCGAC 
CTCTTTGTGG GCGGAGTCCC AGAGGACCAG GCTGACGTGG CCCATGAGCG GACCTCGGTT 
GGCGTGGGCC TGAAGGGCTG CATCCGCCTG CTGGATGTCA ACAATCAGCG CCTGGAGCTG 
GGCCCCTGGC AGGGCGTGGT GTCCCGCAGC TCCGGGGTGG GAGAGTGTGG AGAGCACCCC 
TGCATGCCCA GCCCCTGCCA TGGCGGGGCA CCATGCCAGG CCCTGGGAGA CGACATGTTT 
CGCTGCCAGT GTCCACCCGG CCGCTTTGGC CCAACCTGTG CGGAAGAGAA GAGCCCCTGC 
CAGCCGAACC CCTGCCACGG TGCAGCCCCC TGCCGCGTGC TACCCAAGGG CGGGGCCAAG 
TGCGAGTGCC CCCCGGGACG TAGAGGCACT CTCTGCCAGA CAGTGTCTGA GATGGATGGT 
AGTGCCCAGC CCTTCCTGGC GGACTTCCAT GGCTTTTCCT ACCTGGAGCT GAGAGGCTTG 
CACACCTTTG AGAGAGACCT GGGGGAGAAG ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA 
GGACCCAGCG GTTTGCTCCT GTACAATGGG CAAAAGACAG ATGGCAAAGG GGACTTCGTG 
TCACTGGCCC TGCATGACCA GCACCTGGAA TTCCGCTATG ACCTGGGCAA AGGGGCAGCG 
GTCATCAGGA GCAAGGAACC CATAGCCCTG GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA 
AACGGCCGTA AGGGCGCCCT GCGTGTGGGT GATGGGCCCC GCGTGCTGGG AGAGTCCCCG 
GTGCCGCATA CCATGCTCAA TTTGAAGGAG CCACTCTACA TCGGGGGTGC CCCCGACTTC 
AGCAAGCTGG CCCGGGCAGC TGCAGCATCC TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC 
TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG GAGCACGTGG TGCAGGCGGT GGACGTCTCA 
TCCTTTACAG ACCACCCTTG TACCCAGGCC TCAGGCCACC CCTGCCTCCA TGGCGCCTTG 
TGTCTCCCAA AGGAAGCCAC CTACACATGC CTGTGCCCCA TGGGCTTCTC GGGGCCACAC 
TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA GGGGACCTCG AGGCTCTGGC CTTCGATGGG 
AGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AGAAAGCCCT GCAGACCAAC 
CACTTCGAGC TCAGCTTGCG CACTGAAGCT ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG 
GCCACCGAGC GTGCGGACTA CATGGCGCTA GCCATCGTGG ATGGACACCT TCAGCTGGCC 
TATGACCTGG GCTCTCAGCC TGTGGTGCTG CGCTCCACCG TGAGGGTCAA CACCAACCGC 
TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG AGAGAAGGTT CCCTCCAGGT GGGCAATGAG 
GCCCCCGTGA CTGGCTCCTC CCCACTGGGT GCCACACAGC TGGACACAGA TGGAACTCTC 
TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG GGCCAGGCCC TGCCAAAGGC CTACGGAACA 
GGTTTTGTGG GCTGCCTCCG GGATGTGGTG GTAGGCCAGC GTCCACTGCA CCTGCTGGAA 
GATGCCCTCA CCAAGCCAGA GCTGCGGCCC TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005339328.1
CDS	300..6044
Translation	MLNSSLMRITLRNLEEVEFCVEDKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_005339271.1
Organism	Ictidomys tridecemlineatus(Thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005339271.1

ORF Insert Sequence:

ATGCTCAACT CTAGCCTCAT GCGGATCACC CTTCGCAACC TGGAGGAGGT GGAGTTTTGT 
GTAGAAGACA AACCTGGGAC CCACTTCACA CCAGCACCTC CCATGCCTCC TGACGTCTGC 
AGGGGAATGC TGTGTGGCTT TGGCGCGGTC TGTGAGCCCA GTGCAGAGGA TCCAGGCCGG 
GCCTCCTGCG TGTGCAAGAA GAGCGCCTGT CCTGCCGTCG TGGCGCCAGT GTGCGGCTCA 
GATGCCTCCA CCTACAGCAA CGAGTGCGAG CTGCAGCGTG CACAGTGCCG CCTGCAGCGG 
CGAATCCGCC TGCTCCGCCG CGGACCCTGT GGTTCCCGGG ACCCCTGCTC CAATGTGACC 
TGCAGCTTCG GCAGCACCTG TGCCCCCTCC GCCGATGGGC AGACCGCCTC CTGCCTGTGT 
CCCACGACCT GCCGCGGGGC CCCGGAGGGC ACCGTGTGTG GCAGTGATGG TATGGACTAC 
CCCGGTGAGT GCCAGCTGCT GCGCCGGGCA TGCGCACGCC AGGAGAACAT CTTTAGGAAG 
TTCGACGGCC CCTGTGACCC CTGCCAGGGA GCCCATGCTG ACCCGAGCCG CGTCTGCCGT 
GTGAACCCGC GCACTCGCCG TCCAGAGATG CTCCTGCGCC CAGAGAGCTG CCCTCCCCGG 
CATTTGCCTG TGTGTGGGGA TGATGGGGTC ACCTATGCAA ATGACTGCGT CATGGGCCGC 
TCAGGGGCTG TCCGCGGTCT GCTCCTCCAG AAAGTGCGCT CTGGCCAGTG CCAGCCTCGA 
GACCAGTGCT CCGGGACCTG CCAGTTCAGT GCCGTTTGCC TGTCCCGCCG TGGCCGACCC 
CGCTGCTCCT GCGACCGTGT CACCTGTGAT GGGGCCTACA GACCGGTGTG TGCCCAGGAT 
GGGCACACGT ATGACAACGA CTGTTGGCGC CAGCAGGCTG AGTGCCGGCA GCAGCGGGCC 
ATCCCTCCCA GGCACCAGGG CCCGTGTGAC CAGGCTCCGT CCCCGTGCCG GGGGGTGCAG 
TGCGCATTCG GGGCGACATG CGCCGTGAAG AACGGGGAGG CTGCGTGCGA GTGCCAGCAG 
GCGTGTGCAG GCGCCTACGA CCCCGTGTGC GGCAGTGACG GCGTCACCTA TGGCAGCACC 
TGCGAGCTGG AGGCCATGGC CTGTGCCCTT GGGCGGGATA TCCGGGTGGC CCGCAGAGGA 
CCCTGTGACC GATGCGGGCA GTGCCGTTTT GGAGCCCTGT GCGAGGCCGA GACCGGGCGT 
TGTGTGTGCC CCTCTGAGTG CGTGGCCTCA GCCCAGCCCG TGTGCGGTTC TGATGGGCAC 
ACGTATGCCA GCGAGTGTGA GCTGCATGTC CACGCCTGCA CACACCAGAT AAGCCTGCAT 
GTGGCATCAG CTGGACACTG TCAGACCTGT GGAGACACAG TGTGTGCCTT TGGGGCTGTG 
TGCTCAGCCG GGCAGTGTGT GTGTCCTCGT TGTGAGCGCC CCCCGCCTGG CCCCGTGTGT 
GGCAGCGACG GTGTCACCTA CCGCAGTGCG TGTGAGCTAC GGGAGGCTGC CTGCCAGCAG 
CAGATACAGC TTGAGGAAGC CCGGGCAGGG CCCTGTGAGC AGGCTGAGTG TGGCTCAGGA 
GGCTCCGGCT CTGGGGAGGA CAGCGAGTGT GAGCAGGAAC TCTGCCGGCA GCGAGGCGGG 
GTGTGGGATG AGGACTCAGA GGATGGACCC TGCGTCTGCG ACTTCAGCTG CCAGGGTGTT 
CTCAGGAGCC TGGTGTGCGG CTCAGATGGG GTCACCTATG GCAGTGAGTG TGACCTGAAG 
AGGGCCAGGT GCGAGTCTCA AGGAGAGTTG TACGTCGCAG CTCAGGGACC CTGCCGAGGC 
CTCACCTTGG CCCCACTGCT GCCTGCAGCC CCCGCTCAAT GTGCCCAGAC CCCCTACGGA 
TGCTGCCAGG ATAATGTCAC TGCTGCCCGG GGTGTGGGCC TGGCCGGTTG CCCCAGCACC 
TGCCAGTGCA ACCCTCACGG CTCCTACGGA GGCACCTGTG ACCCAGCCAC AGGCCAGTGC 
TCTTGCCGGC CAGGTGTGGG CGGCCTCAAG TGTGACCGAT GTGAGCCTGG TTTCTGGAAC 
TTCCGTGGCA TTGTCACTGA CGGCCGGAGC GGCTGTACTC CCTGCAGCTG TGACCCCCGG 
GGTGCCGTGC GGGATGACTG TGAGCAGATG ACTGGGCTGT GCTCCTGTAA GCCGGGCGTC 
GCTGGTCCCA AGTGCGGGCA GTGTCCTGAT GGCCACACCC TGGGCCCCAC CGGCTGTGAG 
GCAGATCCTT CAGCACCGGT GACCTGTGCG GAGATGCACT GTGAGTTTGG CGCTTCCTGC 
GTGGAAGAGG CAGGTTCTGC CCACTGCGTC TGCCCAGCCC TCACCTGCCC AGAGGCCAGT 
GCCACCAAGG TCTGTGGATC AGATGGCATC ACCTACGGCA GCGAGTGCCA ACTGAAGACC 
ATCGCCTGCC GCCAGGGCTT GGACATCTTC ACCCAGAGCC TCGGCCCGTG CCAGGAGGCT 
GTGGTTCCTG GCGCTCACCC GACCTCCAGG GCCACGACGG CCCCGGGGCG CTTGCCGAGC 
GAGCCTCTGC TGCCTCCACC CAGTGCCCTC CCCCTGGCTC CCAGCAGTAC CCCCCAGAGC 
CGGCCCACCC CTCCACCCAC CTTACGACCT CGGACCACAG CCAGCCTCCC CAGGAGCACA 
GCTCGGCCGG CGCTGACCAT GCCTCCCATA GAGACCTCCA CTGCAACCAG TCTCTCCGCC 
TCTGCCTTTG GAGAGTCTGG CAGCACTGAT GGGAGTGGCG ATGAGGAACT GAGTGGGGAC 
CAGGAGGCCA GTGGGGGTGG TTCTGGGGGA CTGGAACTCT CCATGGACAG CAGCGTGGTG 
ACACAGGGAC CACCCATGGA GAGAGCCTCC TGCTACAACT CACCTTTGGG CTGCTGTTCG 
GATGGCCAGA CACCTTCGCT GGACGCAGAG GGCTCCAACT GTCCTGCCAC CAAGGTGTAC 
CAGGGCGTGC TGGAGCTGGA GGGGGTCGAG GGGCAAGAGC TGTTCTACAC GCCCGAAATG 
GCCGACCCCA AGTCAGAGCT GTTTGGGGAG ACGGCCAGGA GCATCGAGAG CACTCTGGAC 
GCCCTTTTCC GGAACTCAGA CGTCAAAAAG GACTTCCGGA GTGTCCACCT GCGGGACCTG 
GGGCCTGGCA GATCAGTCCG TGCCACTGTG GATGTGCACT TTGACCCCAC CACAGCCTTC 
AGGGCACCTG ACGTGGGCCG GGCCCTGCTG CGCCAGATCC AGGCCTCCAG GCGCCGGGCC 
CTGGCGGTGC GGAGGCCTCT GCAGGAGCAC GTGCGGTTCA TGGACTTCGA CTGGTTTCCT 
GCGTTCCTCA CGGGAGCCAC CACAGGAGCC ACCGCTGCAG TGGCCACGGC CAGAGCTACC 
ACTGTGGGCC CACCACCTTC CTCCGTGACC CCTCGGGTCC CCCCCAGTCA CACCAGCAGG 
CCCACTGGAA GGACCACAGC ACCCACACAC ACACACCGGC CCCCCACCAC AGCTCCCGGC 
CATGTGGCTG GACGCTGGCC TTTGCTCCCC AATCCCAAGC AGCACCCCAG GTCCTGTGAT 
TCTCAGCCCT GCCTGCATGG GGGGACCTGC CAGGACCAGG ACTCTGGCAG GGGCTTCACC 
TGCAGCTGCC CGGCAGGCCG GGGAGGCACC TTCTGTGAGG ACGTGCTGCG CCCCTCTGTC 
TCAGTTCCTG CGTTTGGGGG TGCCTCCTTT CTGGCCTTCC CCACCCTCCG GGCATATCAC 
ACGCTGCGCC TGGCGCTGGA ATTCCGATCC CTGGAGCCTC AGGGACTGCT GCTGTACAAC 
GGCAACGCCC GTGGCAAGGA CTTCCTGGCC CTGGCGCTGC TCGGTGGGCG CGTGCAGCTC 
AGGTTTGACA CAGGTTCTGG ACCAGCAATA CTGACCAGCT CGGTGCCTGT GGAACCAGGG 
CGGTGGCATC GCCTAGAACT CTCACGGCAC TGGCGCCAAG GCACACTGTC TGTGGATGGC 
GAGACCCCAG TTCTGGGCGA GAGCCCCAGT GGCACGGATG GCCTCAACCT AGATACCGAC 
CTCTTTGTGG GCGGAGTCCC AGAGGACCAG GCTGACGTGG CCCATGAGCG GACCTCGGTT 
GGCGTGGGCC TGAAGGGCTG CATCCGCCTG CTGGATGTCA ACAATCAGCG CCTGGAGCTG 
GGCCCCTGGC AGGGCGTGGT GTCCCGCAGC TCCGGGGTGG GAGAGTGTGG AGAGCACCCC 
TGCATGCCCA GCCCCTGCCA TGGCGGGGCA CCATGCCAGG CCCTGGGAGA CGACATGTTT 
CGCTGCCAGT GTCCACCCGG CCGCTTTGGC CCAACCTGTG CGGAAGAGAA GAGCCCCTGC 
CAGCCGAACC CCTGCCACGG TGCAGCCCCC TGCCGCGTGC TACCCAAGGG CGGGGCCAAG 
TGCGAGTGCC CCCCGGGACG TAGAGGCACT CTCTGCCAGA CAGTGTCTGA GATGGATGGT 
AGTGCCCAGC CCTTCCTGGC GGACTTCCAT GGCTTTTCCT ACCTGGAGCT GAGAGGCTTG 
CACACCTTTG AGAGAGACCT GGGGGAGAAG ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA 
GGACCCAGCG GTTTGCTCCT GTACAATGGG CAAAAGACAG ATGGCAAAGG GGACTTCGTG 
TCACTGGCCC TGCATGACCA GCACCTGGAA TTCCGCTATG ACCTGGGCAA AGGGGCAGCG 
GTCATCAGGA GCAAGGAACC CATAGCCCTG GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA 
AACGGCCGTA AGGGCGCCCT GCGTGTGGGT GATGGGCCCC GCGTGCTGGG AGAGTCCCCG 
GTGCCGCATA CCATGCTCAA TTTGAAGGAG CCACTCTACA TCGGGGGTGC CCCCGACTTC 
AGCAAGCTGG CCCGGGCAGC TGCAGCATCC TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC 
TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG GAGCACGTGG TGCAGGCGGT GGACGTCTCA 
TCCTTTACAG ACCACCCTTG TACCCAGGCC TCAGGCCACC CCTGCCTCCA TGGCGCCTTG 
TGTCTCCCAA AGGAAGCCAC CTACACATGC CTGTGCCCCA TGGGCTTCTC GGGGCCACAC 
TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA GGGGACCTCG AGGCTCTGGC CTTCGATGGG 
AGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AGAAAGCCCT GCAGACCAAC 
CACTTCGAGC TCAGCTTGCG CACTGAAGCT ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG 
GCCACCGAGC GTGCGGACTA CATGGCGCTA GCCATCGTGG ATGGACACCT TCAGCTGGCC 
TATGACCTGG GCTCTCAGCC TGTGGTGCTG CGCTCCACCG TGAGGGTCAA CACCAACCGC 
TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG AGAGAAGGTT CCCTCCAGGT GGGCAATGAG 
GCCCCCGTGA CTGGCTCCTC CCCACTGGGT GCCACACAGC TGGACACAGA TGGAACTCTC 
TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG GGCCAGGCCC TGCCAAAGGC CTACGGAACA 
GGTTTTGTGG GCTGCCTCCG GGATGTGGTG GTAGGCCAGC GTCCACTGCA CCTGCTGGAA 
GATGCCCTCA CCAAGCCAGA GCTGCGGCCC TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_005339271.2 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_005339328.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X10, mRNA.

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CloneID	OIc70064
Clone ID Related Accession (Same CDS sequence)	XM_005339271.1 , XM_005339271.2
Accession Version	XM_005339271.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 3, 2017 this sequence version replaced XM_005339271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAACT CTAGCCTCAT GCGGATCACC CTTCGCAACC TGGAGGAGGT GGAGTTTTGT 
GTAGAAGACA AACCTGGGAC CCACTTCACA CCAGCACCTC CCATGCCTCC TGACGTCTGC 
AGGGGAATGC TGTGTGGCTT TGGCGCGGTC TGTGAGCCCA GTGCAGAGGA TCCAGGCCGG 
GCCTCCTGCG TGTGCAAGAA GAGCGCCTGT CCTGCCGTCG TGGCGCCAGT GTGCGGCTCA 
GATGCCTCCA CCTACAGCAA CGAGTGCGAG CTGCAGCGTG CACAGTGCCG CCTGCAGCGG 
CGAATCCGCC TGCTCCGCCG CGGACCCTGT GGTTCCCGGG ACCCCTGCTC CAATGTGACC 
TGCAGCTTCG GCAGCACCTG TGCCCCCTCC GCCGATGGGC AGACCGCCTC CTGCCTGTGT 
CCCACGACCT GCCGCGGGGC CCCGGAGGGC ACCGTGTGTG GCAGTGATGG TATGGACTAC 
CCCGGTGAGT GCCAGCTGCT GCGCCGGGCA TGCGCACGCC AGGAGAACAT CTTTAGGAAG 
TTCGACGGCC CCTGTGACCC CTGCCAGGGA GCCCATGCTG ACCCGAGCCG CGTCTGCCGT 
GTGAACCCGC GCACTCGCCG TCCAGAGATG CTCCTGCGCC CAGAGAGCTG CCCTCCCCGG 
CATTTGCCTG TGTGTGGGGA TGATGGGGTC ACCTATGCAA ATGACTGCGT CATGGGCCGC 
TCAGGGGCTG TCCGCGGTCT GCTCCTCCAG AAAGTGCGCT CTGGCCAGTG CCAGCCTCGA 
GACCAGTGCT CCGGGACCTG CCAGTTCAGT GCCGTTTGCC TGTCCCGCCG TGGCCGACCC 
CGCTGCTCCT GCGACCGTGT CACCTGTGAT GGGGCCTACA GACCGGTGTG TGCCCAGGAT 
GGGCACACGT ATGACAACGA CTGTTGGCGC CAGCAGGCTG AGTGCCGGCA GCAGCGGGCC 
ATCCCTCCCA GGCACCAGGG CCCGTGTGAC CAGGCTCCGT CCCCGTGCCG GGGGGTGCAG 
TGCGCATTCG GGGCGACATG CGCCGTGAAG AACGGGGAGG CTGCGTGCGA GTGCCAGCAG 
GCGTGTGCAG GCGCCTACGA CCCCGTGTGC GGCAGTGACG GCGTCACCTA TGGCAGCACC 
TGCGAGCTGG AGGCCATGGC CTGTGCCCTT GGGCGGGATA TCCGGGTGGC CCGCAGAGGA 
CCCTGTGACC GATGCGGGCA GTGCCGTTTT GGAGCCCTGT GCGAGGCCGA GACCGGGCGT 
TGTGTGTGCC CCTCTGAGTG CGTGGCCTCA GCCCAGCCCG TGTGCGGTTC TGATGGGCAC 
ACGTATGCCA GCGAGTGTGA GCTGCATGTC CACGCCTGCA CACACCAGAT AAGCCTGCAT 
GTGGCATCAG CTGGACACTG TCAGACCTGT GGAGACACAG TGTGTGCCTT TGGGGCTGTG 
TGCTCAGCCG GGCAGTGTGT GTGTCCTCGT TGTGAGCGCC CCCCGCCTGG CCCCGTGTGT 
GGCAGCGACG GTGTCACCTA CCGCAGTGCG TGTGAGCTAC GGGAGGCTGC CTGCCAGCAG 
CAGATACAGC TTGAGGAAGC CCGGGCAGGG CCCTGTGAGC AGGCTGAGTG TGGCTCAGGA 
GGCTCCGGCT CTGGGGAGGA CAGCGAGTGT GAGCAGGAAC TCTGCCGGCA GCGAGGCGGG 
GTGTGGGATG AGGACTCAGA GGATGGACCC TGCGTCTGCG ACTTCAGCTG CCAGGGTGTT 
CTCAGGAGCC TGGTGTGCGG CTCAGATGGG GTCACCTATG GCAGTGAGTG TGACCTGAAG 
AGGGCCAGGT GCGAGTCTCA AGGAGAGTTG TACGTCGCAG CTCAGGGACC CTGCCGAGGC 
CTCACCTTGG CCCCACTGCT GCCTGCAGCC CCCGCTCAAT GTGCCCAGAC CCCCTACGGA 
TGCTGCCAGG ATAATGTCAC TGCTGCCCGG GGTGTGGGCC TGGCCGGTTG CCCCAGCACC 
TGCCAGTGCA ACCCTCACGG CTCCTACGGA GGCACCTGTG ACCCAGCCAC AGGCCAGTGC 
TCTTGCCGGC CAGGTGTGGG CGGCCTCAAG TGTGACCGAT GTGAGCCTGG TTTCTGGAAC 
TTCCGTGGCA TTGTCACTGA CGGCCGGAGC GGCTGTACTC CCTGCAGCTG TGACCCCCGG 
GGTGCCGTGC GGGATGACTG TGAGCAGATG ACTGGGCTGT GCTCCTGTAA GCCGGGCGTC 
GCTGGTCCCA AGTGCGGGCA GTGTCCTGAT GGCCACACCC TGGGCCCCAC CGGCTGTGAG 
GCAGATCCTT CAGCACCGGT GACCTGTGCG GAGATGCACT GTGAGTTTGG CGCTTCCTGC 
GTGGAAGAGG CAGGTTCTGC CCACTGCGTC TGCCCAGCCC TCACCTGCCC AGAGGCCAGT 
GCCACCAAGG TCTGTGGATC AGATGGCATC ACCTACGGCA GCGAGTGCCA ACTGAAGACC 
ATCGCCTGCC GCCAGGGCTT GGACATCTTC ACCCAGAGCC TCGGCCCGTG CCAGGAGGCT 
GTGGTTCCTG GCGCTCACCC GACCTCCAGG GCCACGACGG CCCCGGGGCG CTTGCCGAGC 
GAGCCTCTGC TGCCTCCACC CAGTGCCCTC CCCCTGGCTC CCAGCAGTAC CCCCCAGAGC 
CGGCCCACCC CTCCACCCAC CTTACGACCT CGGACCACAG CCAGCCTCCC CAGGAGCACA 
GCTCGGCCGG CGCTGACCAT GCCTCCCATA GAGACCTCCA CTGCAACCAG TCTCTCCGCC 
TCTGCCTTTG GAGAGTCTGG CAGCACTGAT GGGAGTGGCG ATGAGGAACT GAGTGGGGAC 
CAGGAGGCCA GTGGGGGTGG TTCTGGGGGA CTGGAACTCT CCATGGACAG CAGCGTGGTG 
ACACAGGGAC CACCCATGGA GAGAGCCTCC TGCTACAACT CACCTTTGGG CTGCTGTTCG 
GATGGCCAGA CACCTTCGCT GGACGCAGAG GGCTCCAACT GTCCTGCCAC CAAGGTGTAC 
CAGGGCGTGC TGGAGCTGGA GGGGGTCGAG GGGCAAGAGC TGTTCTACAC GCCCGAAATG 
GCCGACCCCA AGTCAGAGCT GTTTGGGGAG ACGGCCAGGA GCATCGAGAG CACTCTGGAC 
GCCCTTTTCC GGAACTCAGA CGTCAAAAAG GACTTCCGGA GTGTCCACCT GCGGGACCTG 
GGGCCTGGCA GATCAGTCCG TGCCACTGTG GATGTGCACT TTGACCCCAC CACAGCCTTC 
AGGGCACCTG ACGTGGGCCG GGCCCTGCTG CGCCAGATCC AGGCCTCCAG GCGCCGGGCC 
CTGGCGGTGC GGAGGCCTCT GCAGGAGCAC GTGCGGTTCA TGGACTTCGA CTGGTTTCCT 
GCGTTCCTCA CGGGAGCCAC CACAGGAGCC ACCGCTGCAG TGGCCACGGC CAGAGCTACC 
ACTGTGGGCC CACCACCTTC CTCCGTGACC CCTCGGGTCC CCCCCAGTCA CACCAGCAGG 
CCCACTGGAA GGACCACAGC ACCCACACAC ACACACCGGC CCCCCACCAC AGCTCCCGGC 
CATGTGGCTG GACGCTGGCC TTTGCTCCCC AATCCCAAGC AGCACCCCAG GTCCTGTGAT 
TCTCAGCCCT GCCTGCATGG GGGGACCTGC CAGGACCAGG ACTCTGGCAG GGGCTTCACC 
TGCAGCTGCC CGGCAGGCCG GGGAGGCACC TTCTGTGAGG ACGTGCTGCG CCCCTCTGTC 
TCAGTTCCTG CGTTTGGGGG TGCCTCCTTT CTGGCCTTCC CCACCCTCCG GGCATATCAC 
ACGCTGCGCC TGGCGCTGGA ATTCCGATCC CTGGAGCCTC AGGGACTGCT GCTGTACAAC 
GGCAACGCCC GTGGCAAGGA CTTCCTGGCC CTGGCGCTGC TCGGTGGGCG CGTGCAGCTC 
AGGTTTGACA CAGGTTCTGG ACCAGCAATA CTGACCAGCT CGGTGCCTGT GGAACCAGGG 
CGGTGGCATC GCCTAGAACT CTCACGGCAC TGGCGCCAAG GCACACTGTC TGTGGATGGC 
GAGACCCCAG TTCTGGGCGA GAGCCCCAGT GGCACGGATG GCCTCAACCT AGATACCGAC 
CTCTTTGTGG GCGGAGTCCC AGAGGACCAG GCTGACGTGG CCCATGAGCG GACCTCGGTT 
GGCGTGGGCC TGAAGGGCTG CATCCGCCTG CTGGATGTCA ACAATCAGCG CCTGGAGCTG 
GGCCCCTGGC AGGGCGTGGT GTCCCGCAGC TCCGGGGTGG GAGAGTGTGG AGAGCACCCC 
TGCATGCCCA GCCCCTGCCA TGGCGGGGCA CCATGCCAGG CCCTGGGAGA CGACATGTTT 
CGCTGCCAGT GTCCACCCGG CCGCTTTGGC CCAACCTGTG CGGAAGAGAA GAGCCCCTGC 
CAGCCGAACC CCTGCCACGG TGCAGCCCCC TGCCGCGTGC TACCCAAGGG CGGGGCCAAG 
TGCGAGTGCC CCCCGGGACG TAGAGGCACT CTCTGCCAGA CAGTGTCTGA GATGGATGGT 
AGTGCCCAGC CCTTCCTGGC GGACTTCCAT GGCTTTTCCT ACCTGGAGCT GAGAGGCTTG 
CACACCTTTG AGAGAGACCT GGGGGAGAAG ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA 
GGACCCAGCG GTTTGCTCCT GTACAATGGG CAAAAGACAG ATGGCAAAGG GGACTTCGTG 
TCACTGGCCC TGCATGACCA GCACCTGGAA TTCCGCTATG ACCTGGGCAA AGGGGCAGCG 
GTCATCAGGA GCAAGGAACC CATAGCCCTG GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA 
AACGGCCGTA AGGGCGCCCT GCGTGTGGGT GATGGGCCCC GCGTGCTGGG AGAGTCCCCG 
GTGCCGCATA CCATGCTCAA TTTGAAGGAG CCACTCTACA TCGGGGGTGC CCCCGACTTC 
AGCAAGCTGG CCCGGGCAGC TGCAGCATCC TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC 
TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG GAGCACGTGG TGCAGGCGGT GGACGTCTCA 
TCCTTTACAG ACCACCCTTG TACCCAGGCC TCAGGCCACC CCTGCCTCCA TGGCGCCTTG 
TGTCTCCCAA AGGAAGCCAC CTACACATGC CTGTGCCCCA TGGGCTTCTC GGGGCCACAC 
TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA GGGGACCTCG AGGCTCTGGC CTTCGATGGG 
AGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AGAAAGCCCT GCAGACCAAC 
CACTTCGAGC TCAGCTTGCG CACTGAAGCT ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG 
GCCACCGAGC GTGCGGACTA CATGGCGCTA GCCATCGTGG ATGGACACCT TCAGCTGGCC 
TATGACCTGG GCTCTCAGCC TGTGGTGCTG CGCTCCACCG TGAGGGTCAA CACCAACCGC 
TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG AGAGAAGGTT CCCTCCAGGT GGGCAATGAG 
GCCCCCGTGA CTGGCTCCTC CCCACTGGGT GCCACACAGC TGGACACAGA TGGAACTCTC 
TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG GGCCAGGCCC TGCCAAAGGC CTACGGAACA 
GGTTTTGTGG GCTGCCTCCG GGATGTGGTG GTAGGCCAGC GTCCACTGCA CCTGCTGGAA 
GATGCCCTCA CCAAGCCAGA GCTGCGGCCC TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005339328.1
CDS	300..6044
Translation	MLNSSLMRITLRNLEEVEFCVEDKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_005339271.2
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005339271.2

ORF Insert Sequence:

ATGCTCAACT CTAGCCTCAT GCGGATCACC CTTCGCAACC TGGAGGAGGT GGAGTTTTGT 
GTAGAAGACA AACCTGGGAC CCACTTCACA CCAGCACCTC CCATGCCTCC TGACGTCTGC 
AGGGGAATGC TGTGTGGCTT TGGCGCGGTC TGTGAGCCCA GTGCAGAGGA TCCAGGCCGG 
GCCTCCTGCG TGTGCAAGAA GAGCGCCTGT CCTGCCGTCG TGGCGCCAGT GTGCGGCTCA 
GATGCCTCCA CCTACAGCAA CGAGTGCGAG CTGCAGCGTG CACAGTGCCG CCTGCAGCGG 
CGAATCCGCC TGCTCCGCCG CGGACCCTGT GGTTCCCGGG ACCCCTGCTC CAATGTGACC 
TGCAGCTTCG GCAGCACCTG TGCCCCCTCC GCCGATGGGC AGACCGCCTC CTGCCTGTGT 
CCCACGACCT GCCGCGGGGC CCCGGAGGGC ACCGTGTGTG GCAGTGATGG TATGGACTAC 
CCCGGTGAGT GCCAGCTGCT GCGCCGGGCA TGCGCACGCC AGGAGAACAT CTTTAGGAAG 
TTCGACGGCC CCTGTGACCC CTGCCAGGGA GCCCATGCTG ACCCGAGCCG CGTCTGCCGT 
GTGAACCCGC GCACTCGCCG TCCAGAGATG CTCCTGCGCC CAGAGAGCTG CCCTCCCCGG 
CATTTGCCTG TGTGTGGGGA TGATGGGGTC ACCTATGCAA ATGACTGCGT CATGGGCCGC 
TCAGGGGCTG TCCGCGGTCT GCTCCTCCAG AAAGTGCGCT CTGGCCAGTG CCAGCCTCGA 
GACCAGTGCT CCGGGACCTG CCAGTTCAGT GCCGTTTGCC TGTCCCGCCG TGGCCGACCC 
CGCTGCTCCT GCGACCGTGT CACCTGTGAT GGGGCCTACA GACCGGTGTG TGCCCAGGAT 
GGGCACACGT ATGACAACGA CTGTTGGCGC CAGCAGGCTG AGTGCCGGCA GCAGCGGGCC 
ATCCCTCCCA GGCACCAGGG CCCGTGTGAC CAGGCTCCGT CCCCGTGCCG GGGGGTGCAG 
TGCGCATTCG GGGCGACATG CGCCGTGAAG AACGGGGAGG CTGCGTGCGA GTGCCAGCAG 
GCGTGTGCAG GCGCCTACGA CCCCGTGTGC GGCAGTGACG GCGTCACCTA TGGCAGCACC 
TGCGAGCTGG AGGCCATGGC CTGTGCCCTT GGGCGGGATA TCCGGGTGGC CCGCAGAGGA 
CCCTGTGACC GATGCGGGCA GTGCCGTTTT GGAGCCCTGT GCGAGGCCGA GACCGGGCGT 
TGTGTGTGCC CCTCTGAGTG CGTGGCCTCA GCCCAGCCCG TGTGCGGTTC TGATGGGCAC 
ACGTATGCCA GCGAGTGTGA GCTGCATGTC CACGCCTGCA CACACCAGAT AAGCCTGCAT 
GTGGCATCAG CTGGACACTG TCAGACCTGT GGAGACACAG TGTGTGCCTT TGGGGCTGTG 
TGCTCAGCCG GGCAGTGTGT GTGTCCTCGT TGTGAGCGCC CCCCGCCTGG CCCCGTGTGT 
GGCAGCGACG GTGTCACCTA CCGCAGTGCG TGTGAGCTAC GGGAGGCTGC CTGCCAGCAG 
CAGATACAGC TTGAGGAAGC CCGGGCAGGG CCCTGTGAGC AGGCTGAGTG TGGCTCAGGA 
GGCTCCGGCT CTGGGGAGGA CAGCGAGTGT GAGCAGGAAC TCTGCCGGCA GCGAGGCGGG 
GTGTGGGATG AGGACTCAGA GGATGGACCC TGCGTCTGCG ACTTCAGCTG CCAGGGTGTT 
CTCAGGAGCC TGGTGTGCGG CTCAGATGGG GTCACCTATG GCAGTGAGTG TGACCTGAAG 
AGGGCCAGGT GCGAGTCTCA AGGAGAGTTG TACGTCGCAG CTCAGGGACC CTGCCGAGGC 
CTCACCTTGG CCCCACTGCT GCCTGCAGCC CCCGCTCAAT GTGCCCAGAC CCCCTACGGA 
TGCTGCCAGG ATAATGTCAC TGCTGCCCGG GGTGTGGGCC TGGCCGGTTG CCCCAGCACC 
TGCCAGTGCA ACCCTCACGG CTCCTACGGA GGCACCTGTG ACCCAGCCAC AGGCCAGTGC 
TCTTGCCGGC CAGGTGTGGG CGGCCTCAAG TGTGACCGAT GTGAGCCTGG TTTCTGGAAC 
TTCCGTGGCA TTGTCACTGA CGGCCGGAGC GGCTGTACTC CCTGCAGCTG TGACCCCCGG 
GGTGCCGTGC GGGATGACTG TGAGCAGATG ACTGGGCTGT GCTCCTGTAA GCCGGGCGTC 
GCTGGTCCCA AGTGCGGGCA GTGTCCTGAT GGCCACACCC TGGGCCCCAC CGGCTGTGAG 
GCAGATCCTT CAGCACCGGT GACCTGTGCG GAGATGCACT GTGAGTTTGG CGCTTCCTGC 
GTGGAAGAGG CAGGTTCTGC CCACTGCGTC TGCCCAGCCC TCACCTGCCC AGAGGCCAGT 
GCCACCAAGG TCTGTGGATC AGATGGCATC ACCTACGGCA GCGAGTGCCA ACTGAAGACC 
ATCGCCTGCC GCCAGGGCTT GGACATCTTC ACCCAGAGCC TCGGCCCGTG CCAGGAGGCT 
GTGGTTCCTG GCGCTCACCC GACCTCCAGG GCCACGACGG CCCCGGGGCG CTTGCCGAGC 
GAGCCTCTGC TGCCTCCACC CAGTGCCCTC CCCCTGGCTC CCAGCAGTAC CCCCCAGAGC 
CGGCCCACCC CTCCACCCAC CTTACGACCT CGGACCACAG CCAGCCTCCC CAGGAGCACA 
GCTCGGCCGG CGCTGACCAT GCCTCCCATA GAGACCTCCA CTGCAACCAG TCTCTCCGCC 
TCTGCCTTTG GAGAGTCTGG CAGCACTGAT GGGAGTGGCG ATGAGGAACT GAGTGGGGAC 
CAGGAGGCCA GTGGGGGTGG TTCTGGGGGA CTGGAACTCT CCATGGACAG CAGCGTGGTG 
ACACAGGGAC CACCCATGGA GAGAGCCTCC TGCTACAACT CACCTTTGGG CTGCTGTTCG 
GATGGCCAGA CACCTTCGCT GGACGCAGAG GGCTCCAACT GTCCTGCCAC CAAGGTGTAC 
CAGGGCGTGC TGGAGCTGGA GGGGGTCGAG GGGCAAGAGC TGTTCTACAC GCCCGAAATG 
GCCGACCCCA AGTCAGAGCT GTTTGGGGAG ACGGCCAGGA GCATCGAGAG CACTCTGGAC 
GCCCTTTTCC GGAACTCAGA CGTCAAAAAG GACTTCCGGA GTGTCCACCT GCGGGACCTG 
GGGCCTGGCA GATCAGTCCG TGCCACTGTG GATGTGCACT TTGACCCCAC CACAGCCTTC 
AGGGCACCTG ACGTGGGCCG GGCCCTGCTG CGCCAGATCC AGGCCTCCAG GCGCCGGGCC 
CTGGCGGTGC GGAGGCCTCT GCAGGAGCAC GTGCGGTTCA TGGACTTCGA CTGGTTTCCT 
GCGTTCCTCA CGGGAGCCAC CACAGGAGCC ACCGCTGCAG TGGCCACGGC CAGAGCTACC 
ACTGTGGGCC CACCACCTTC CTCCGTGACC CCTCGGGTCC CCCCCAGTCA CACCAGCAGG 
CCCACTGGAA GGACCACAGC ACCCACACAC ACACACCGGC CCCCCACCAC AGCTCCCGGC 
CATGTGGCTG GACGCTGGCC TTTGCTCCCC AATCCCAAGC AGCACCCCAG GTCCTGTGAT 
TCTCAGCCCT GCCTGCATGG GGGGACCTGC CAGGACCAGG ACTCTGGCAG GGGCTTCACC 
TGCAGCTGCC CGGCAGGCCG GGGAGGCACC TTCTGTGAGG ACGTGCTGCG CCCCTCTGTC 
TCAGTTCCTG CGTTTGGGGG TGCCTCCTTT CTGGCCTTCC CCACCCTCCG GGCATATCAC 
ACGCTGCGCC TGGCGCTGGA ATTCCGATCC CTGGAGCCTC AGGGACTGCT GCTGTACAAC 
GGCAACGCCC GTGGCAAGGA CTTCCTGGCC CTGGCGCTGC TCGGTGGGCG CGTGCAGCTC 
AGGTTTGACA CAGGTTCTGG ACCAGCAATA CTGACCAGCT CGGTGCCTGT GGAACCAGGG 
CGGTGGCATC GCCTAGAACT CTCACGGCAC TGGCGCCAAG GCACACTGTC TGTGGATGGC 
GAGACCCCAG TTCTGGGCGA GAGCCCCAGT GGCACGGATG GCCTCAACCT AGATACCGAC 
CTCTTTGTGG GCGGAGTCCC AGAGGACCAG GCTGACGTGG CCCATGAGCG GACCTCGGTT 
GGCGTGGGCC TGAAGGGCTG CATCCGCCTG CTGGATGTCA ACAATCAGCG CCTGGAGCTG 
GGCCCCTGGC AGGGCGTGGT GTCCCGCAGC TCCGGGGTGG GAGAGTGTGG AGAGCACCCC 
TGCATGCCCA GCCCCTGCCA TGGCGGGGCA CCATGCCAGG CCCTGGGAGA CGACATGTTT 
CGCTGCCAGT GTCCACCCGG CCGCTTTGGC CCAACCTGTG CGGAAGAGAA GAGCCCCTGC 
CAGCCGAACC CCTGCCACGG TGCAGCCCCC TGCCGCGTGC TACCCAAGGG CGGGGCCAAG 
TGCGAGTGCC CCCCGGGACG TAGAGGCACT CTCTGCCAGA CAGTGTCTGA GATGGATGGT 
AGTGCCCAGC CCTTCCTGGC GGACTTCCAT GGCTTTTCCT ACCTGGAGCT GAGAGGCTTG 
CACACCTTTG AGAGAGACCT GGGGGAGAAG ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA 
GGACCCAGCG GTTTGCTCCT GTACAATGGG CAAAAGACAG ATGGCAAAGG GGACTTCGTG 
TCACTGGCCC TGCATGACCA GCACCTGGAA TTCCGCTATG ACCTGGGCAA AGGGGCAGCG 
GTCATCAGGA GCAAGGAACC CATAGCCCTG GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA 
AACGGCCGTA AGGGCGCCCT GCGTGTGGGT GATGGGCCCC GCGTGCTGGG AGAGTCCCCG 
GTGCCGCATA CCATGCTCAA TTTGAAGGAG CCACTCTACA TCGGGGGTGC CCCCGACTTC 
AGCAAGCTGG CCCGGGCAGC TGCAGCATCC TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC 
TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG GAGCACGTGG TGCAGGCGGT GGACGTCTCA 
TCCTTTACAG ACCACCCTTG TACCCAGGCC TCAGGCCACC CCTGCCTCCA TGGCGCCTTG 
TGTCTCCCAA AGGAAGCCAC CTACACATGC CTGTGCCCCA TGGGCTTCTC GGGGCCACAC 
TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA GGGGACCTCG AGGCTCTGGC CTTCGATGGG 
AGGACCTACA TCGAGTACCT CAACGCTGTG ACCGAGAGCG AGAAAGCCCT GCAGACCAAC 
CACTTCGAGC TCAGCTTGCG CACTGAAGCT ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG 
GCCACCGAGC GTGCGGACTA CATGGCGCTA GCCATCGTGG ATGGACACCT TCAGCTGGCC 
TATGACCTGG GCTCTCAGCC TGTGGTGCTG CGCTCCACCG TGAGGGTCAA CACCAACCGC 
TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG AGAGAAGGTT CCCTCCAGGT GGGCAATGAG 
GCCCCCGTGA CTGGCTCCTC CCCACTGGGT GCCACACAGC TGGACACAGA TGGAACTCTC 
TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG GGCCAGGCCC TGCCAAAGGC CTACGGAACA 
GGTTTTGTGG GCTGCCTCCG GGATGTGGTG GTAGGCCAGC GTCCACTGCA CCTGCTGGAA 
GATGCCCTCA CCAAGCCAGA GCTGCGGCCC TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735915.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591590.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X1, mRNA.

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CloneID	OIc84642
Clone ID Related Accession (Same CDS sequence)	XM_021735915.1
Accession Version	XM_021735915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CGAACTGACC AATGAGATCC CAGTCCCCGA AACTCCGGAT 
TCTGGGGCCC TAAACAGCGA GAAAGCCCTG CAGACCAACC ACTTCGAGCT CAGCTTGCGC 
ACTGAAGCTA CGCAGGGGCT GGTGCTGTGG AGTGGCAAGG CCACCGAGCG TGCGGACTAC 
ATGGCGCTAG CCATCGTGGA TGGACACCTT CAGCTGGCCT ATGACCTGGG CTCTCAGCCT 
GTGGTGCTGC GCTCCACCGT GAGGGTCAAC ACCAACCGCT GGTTGCGAGT CAGGGCTCAC 
AGGGAGCAGA GAGAAGGTTC CCTCCAGGTG GGCAATGAGG CCCCCGTGAC TGGCTCCTCC 
CCACTGGGTG CCACACAGCT GGACACAGAT GGAACTCTCT GGCTCGGGGG CCTGCAGAAG 
CTGCCTGTGG GCCAGGCCCT GCCAAAGGCC TACGGAACAG GTTTTGTGGG CTGCCTCCGG 
GATGTGGTGG TAGGCCAGCG TCCACTGCAC CTGCTGGAAG ATGCCCTCAC CAAGCCAGAG 
CTGCGGCCCT GCCCAGCCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591590.1
CDS	238..6141
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGSVGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESELTNEIPVPETPDSGALNSEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735915.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735915.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CGAACTGACC AATGAGATCC CAGTCCCCGA AACTCCGGAT 
TCTGGGGCCC TAAACAGCGA GAAAGCCCTG CAGACCAACC ACTTCGAGCT CAGCTTGCGC 
ACTGAAGCTA CGCAGGGGCT GGTGCTGTGG AGTGGCAAGG CCACCGAGCG TGCGGACTAC 
ATGGCGCTAG CCATCGTGGA TGGACACCTT CAGCTGGCCT ATGACCTGGG CTCTCAGCCT 
GTGGTGCTGC GCTCCACCGT GAGGGTCAAC ACCAACCGCT GGTTGCGAGT CAGGGCTCAC 
AGGGAGCAGA GAGAAGGTTC CCTCCAGGTG GGCAATGAGG CCCCCGTGAC TGGCTCCTCC 
CCACTGGGTG CCACACAGCT GGACACAGAT GGAACTCTCT GGCTCGGGGG CCTGCAGAAG 
CTGCCTGTGG GCCAGGCCCT GCCAAAGGCC TACGGAACAG GTTTTGTGGG CTGCCTCCGG 
GATGTGGTGG TAGGCCAGCG TCCACTGCAC CTGCTGGAAG ATGCCCTCAC CAAGCCAGAG 
CTGCGGCCCT GCCCAGCCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735916.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591591.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIc84643
Clone ID Related Accession (Same CDS sequence)	XM_021735916.1
Accession Version	XM_021735916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GAAATCCCGC AAGGTGCCGC ATACCATGCT CAATTTGAAG 
GAGCCACTCT ACATCGGGGG TGCCCCCGAC TTCAGCAAGC TGGCCCGGGC AGCTGCAGCA 
TCCTCTGGCT TCGACGGTGC CGTTCAGCTG GTCTCCTTGG GAGGCCGCCA GCTGTTGGCC 
CAGGAGCACG TGGTGCAGGC GGTGGACGTC TCATCCTTTA CAGACCACCC TTGTACCCAG 
GCCTCAGGCC ACCCCTGCCT CCATGGCGCC TTGTGTCTCC CAAAGGAAGC CACCTACACA 
TGCCTGTGCC CCATGGGCTT CTCGGGGCCA CACTGCGAGA AGGGGCTGGT TGAGAAGTCA 
GCAGGGGACC TCGAGGCTCT GGCCTTCGAT GGGAGGACCT ACATCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAACTGAC CAATGAGATC CCAGTCCCCG AAACTCCGGA TTCTGGGGCC 
CTAAACAGCG AGAAAGCCCT GCAGACCAAC CACTTCGAGC TCAGCTTGCG CACTGAAGCT 
ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG GCCACCGAGC GTGCGGACTA CATGGCGCTA 
GCCATCGTGG ATGGACACCT TCAGCTGGCC TATGACCTGG GCTCTCAGCC TGTGGTGCTG 
CGCTCCACCG TGAGGGTCAA CACCAACCGC TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG 
AGAGAAGGTT CCCTCCAGGT GGGCAATGAG GCCCCCGTGA CTGGCTCCTC CCCACTGGGT 
GCCACACAGC TGGACACAGA TGGAACTCTC TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCAAAGGC CTACGGAACA GGTTTTGTGG GCTGCCTCCG GGATGTGGTG 
GTAGGCCAGC GTCCACTGCA CCTGCTGGAA GATGCCCTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591591.1
CDS	1..5895
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESELTNEIPVPETPDSGALNSEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735916.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735916.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GAAATCCCGC AAGGTGCCGC ATACCATGCT CAATTTGAAG 
GAGCCACTCT ACATCGGGGG TGCCCCCGAC TTCAGCAAGC TGGCCCGGGC AGCTGCAGCA 
TCCTCTGGCT TCGACGGTGC CGTTCAGCTG GTCTCCTTGG GAGGCCGCCA GCTGTTGGCC 
CAGGAGCACG TGGTGCAGGC GGTGGACGTC TCATCCTTTA CAGACCACCC TTGTACCCAG 
GCCTCAGGCC ACCCCTGCCT CCATGGCGCC TTGTGTCTCC CAAAGGAAGC CACCTACACA 
TGCCTGTGCC CCATGGGCTT CTCGGGGCCA CACTGCGAGA AGGGGCTGGT TGAGAAGTCA 
GCAGGGGACC TCGAGGCTCT GGCCTTCGAT GGGAGGACCT ACATCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAACTGAC CAATGAGATC CCAGTCCCCG AAACTCCGGA TTCTGGGGCC 
CTAAACAGCG AGAAAGCCCT GCAGACCAAC CACTTCGAGC TCAGCTTGCG CACTGAAGCT 
ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG GCCACCGAGC GTGCGGACTA CATGGCGCTA 
GCCATCGTGG ATGGACACCT TCAGCTGGCC TATGACCTGG GCTCTCAGCC TGTGGTGCTG 
CGCTCCACCG TGAGGGTCAA CACCAACCGC TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG 
AGAGAAGGTT CCCTCCAGGT GGGCAATGAG GCCCCCGTGA CTGGCTCCTC CCCACTGGGT 
GCCACACAGC TGGACACAGA TGGAACTCTC TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCAAAGGC CTACGGAACA GGTTTTGTGG GCTGCCTCCG GGATGTGGTG 
GTAGGCCAGC GTCCACTGCA CCTGCTGGAA GATGCCCTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735917.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591592.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X3, mRNA.

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CloneID	OIc84644
Clone ID Related Accession (Same CDS sequence)	XM_021735917.1
Accession Version	XM_021735917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG GTGCCGCATA CCATGCTCAA TTTGAAGGAG 
CCACTCTACA TCGGGGGTGC CCCCGACTTC AGCAAGCTGG CCCGGGCAGC TGCAGCATCC 
TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG 
GAGCACGTGG TGCAGGCGGT GGACGTCTCA TCCTTTACAG ACCACCCTTG TACCCAGGCC 
TCAGGCCACC CCTGCCTCCA TGGCGCCTTG TGTCTCCCAA AGGAAGCCAC CTACACATGC 
CTGTGCCCCA TGGGCTTCTC GGGGCCACAC TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA 
GGGGACCTCG AGGCTCTGGC CTTCGATGGG AGGACCTACA TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AACTGACCAA TGAGATCCCA GTCCCCGAAA CTCCGGATTC TGGGGCCCTA 
AACAGCGAGA AAGCCCTGCA GACCAACCAC TTCGAGCTCA GCTTGCGCAC TGAAGCTACG 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACCGAGCGTG CGGACTACAT GGCGCTAGCC 
ATCGTGGATG GACACCTTCA GCTGGCCTAT GACCTGGGCT CTCAGCCTGT GGTGCTGCGC 
TCCACCGTGA GGGTCAACAC CAACCGCTGG TTGCGAGTCA GGGCTCACAG GGAGCAGAGA 
GAAGGTTCCC TCCAGGTGGG CAATGAGGCC CCCGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACACAGCTGG ACACAGATGG AACTCTCTGG CTCGGGGGCC TGCAGAAGCT GCCTGTGGGC 
CAGGCCCTGC CAAAGGCCTA CGGAACAGGT TTTGTGGGCT GCCTCCGGGA TGTGGTGGTA 
GGCCAGCGTC CACTGCACCT GCTGGAAGAT GCCCTCACCA AGCCAGAGCT GCGGCCCTGC 
CCAGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591592.1
CDS	238..6129
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGSVGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESELTNEIPVPETPDSGALNSEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735917.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735917.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG GTGCCGCATA CCATGCTCAA TTTGAAGGAG 
CCACTCTACA TCGGGGGTGC CCCCGACTTC AGCAAGCTGG CCCGGGCAGC TGCAGCATCC 
TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG 
GAGCACGTGG TGCAGGCGGT GGACGTCTCA TCCTTTACAG ACCACCCTTG TACCCAGGCC 
TCAGGCCACC CCTGCCTCCA TGGCGCCTTG TGTCTCCCAA AGGAAGCCAC CTACACATGC 
CTGTGCCCCA TGGGCTTCTC GGGGCCACAC TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA 
GGGGACCTCG AGGCTCTGGC CTTCGATGGG AGGACCTACA TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AACTGACCAA TGAGATCCCA GTCCCCGAAA CTCCGGATTC TGGGGCCCTA 
AACAGCGAGA AAGCCCTGCA GACCAACCAC TTCGAGCTCA GCTTGCGCAC TGAAGCTACG 
CAGGGGCTGG TGCTGTGGAG TGGCAAGGCC ACCGAGCGTG CGGACTACAT GGCGCTAGCC 
ATCGTGGATG GACACCTTCA GCTGGCCTAT GACCTGGGCT CTCAGCCTGT GGTGCTGCGC 
TCCACCGTGA GGGTCAACAC CAACCGCTGG TTGCGAGTCA GGGCTCACAG GGAGCAGAGA 
GAAGGTTCCC TCCAGGTGGG CAATGAGGCC CCCGTGACTG GCTCCTCCCC ACTGGGTGCC 
ACACAGCTGG ACACAGATGG AACTCTCTGG CTCGGGGGCC TGCAGAAGCT GCCTGTGGGC 
CAGGCCCTGC CAAAGGCCTA CGGAACAGGT TTTGTGGGCT GCCTCCGGGA TGTGGTGGTA 
GGCCAGCGTC CACTGCACCT GCTGGAAGAT GCCCTCACCA AGCCAGAGCT GCGGCCCTGC 
CCAGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735918.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591593.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X4, mRNA.

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CloneID	OIc84645
Clone ID Related Accession (Same CDS sequence)	XM_021735918.1
Accession Version	XM_021735918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CCCCGAAACT CCGGATTCTG GGGCCCTAAA CAGCGAGAAA 
GCCCTGCAGA CCAACCACTT CGAGCTCAGC TTGCGCACTG AAGCTACGCA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC CGAGCGTGCG GACTACATGG CGCTAGCCAT CGTGGATGGA 
CACCTTCAGC TGGCCTATGA CCTGGGCTCT CAGCCTGTGG TGCTGCGCTC CACCGTGAGG 
GTCAACACCA ACCGCTGGTT GCGAGTCAGG GCTCACAGGG AGCAGAGAGA AGGTTCCCTC 
CAGGTGGGCA ATGAGGCCCC CGTGACTGGC TCCTCCCCAC TGGGTGCCAC ACAGCTGGAC 
ACAGATGGAA CTCTCTGGCT CGGGGGCCTG CAGAAGCTGC CTGTGGGCCA GGCCCTGCCA 
AAGGCCTACG GAACAGGTTT TGTGGGCTGC CTCCGGGATG TGGTGGTAGG CCAGCGTCCA 
CTGCACCTGC TGGAAGATGC CCTCACCAAG CCAGAGCTGC GGCCCTGCCC AGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591593.1
CDS	238..6117
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGSVGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESPETPDSGALNSEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735918.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735918.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CCCCGAAACT CCGGATTCTG GGGCCCTAAA CAGCGAGAAA 
GCCCTGCAGA CCAACCACTT CGAGCTCAGC TTGCGCACTG AAGCTACGCA GGGGCTGGTG 
CTGTGGAGTG GCAAGGCCAC CGAGCGTGCG GACTACATGG CGCTAGCCAT CGTGGATGGA 
CACCTTCAGC TGGCCTATGA CCTGGGCTCT CAGCCTGTGG TGCTGCGCTC CACCGTGAGG 
GTCAACACCA ACCGCTGGTT GCGAGTCAGG GCTCACAGGG AGCAGAGAGA AGGTTCCCTC 
CAGGTGGGCA ATGAGGCCCC CGTGACTGGC TCCTCCCCAC TGGGTGCCAC ACAGCTGGAC 
ACAGATGGAA CTCTCTGGCT CGGGGGCCTG CAGAAGCTGC CTGTGGGCCA GGCCCTGCCA 
AAGGCCTACG GAACAGGTTT TGTGGGCTGC CTCCGGGATG TGGTGGTAGG CCAGCGTCCA 
CTGCACCTGC TGGAAGATGC CCTCACCAAG CCAGAGCTGC GGCCCTGCCC AGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735919.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591594.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X5, mRNA.

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CloneID	OIc84646
Clone ID Related Accession (Same CDS sequence)	XM_021735919.1
Accession Version	XM_021735919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CGAACTGACC AATGAGATCC CAGTCGAGAA AGCCCTGCAG 
ACCAACCACT TCGAGCTCAG CTTGCGCACT GAAGCTACGC AGGGGCTGGT GCTGTGGAGT 
GGCAAGGCCA CCGAGCGTGC GGACTACATG GCGCTAGCCA TCGTGGATGG ACACCTTCAG 
CTGGCCTATG ACCTGGGCTC TCAGCCTGTG GTGCTGCGCT CCACCGTGAG GGTCAACACC 
AACCGCTGGT TGCGAGTCAG GGCTCACAGG GAGCAGAGAG AAGGTTCCCT CCAGGTGGGC 
AATGAGGCCC CCGTGACTGG CTCCTCCCCA CTGGGTGCCA CACAGCTGGA CACAGATGGA 
ACTCTCTGGC TCGGGGGCCT GCAGAAGCTG CCTGTGGGCC AGGCCCTGCC AAAGGCCTAC 
GGAACAGGTT TTGTGGGCTG CCTCCGGGAT GTGGTGGTAG GCCAGCGTCC ACTGCACCTG 
CTGGAAGATG CCCTCACCAA GCCAGAGCTG CGGCCCTGCC CAGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591594.1
CDS	238..6108
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGSVGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESELTNEIPVEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735919.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735919.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CGAACTGACC AATGAGATCC CAGTCGAGAA AGCCCTGCAG 
ACCAACCACT TCGAGCTCAG CTTGCGCACT GAAGCTACGC AGGGGCTGGT GCTGTGGAGT 
GGCAAGGCCA CCGAGCGTGC GGACTACATG GCGCTAGCCA TCGTGGATGG ACACCTTCAG 
CTGGCCTATG ACCTGGGCTC TCAGCCTGTG GTGCTGCGCT CCACCGTGAG GGTCAACACC 
AACCGCTGGT TGCGAGTCAG GGCTCACAGG GAGCAGAGAG AAGGTTCCCT CCAGGTGGGC 
AATGAGGCCC CCGTGACTGG CTCCTCCCCA CTGGGTGCCA CACAGCTGGA CACAGATGGA 
ACTCTCTGGC TCGGGGGCCT GCAGAAGCTG CCTGTGGGCC AGGCCCTGCC AAAGGCCTAC 
GGAACAGGTT TTGTGGGCTG CCTCCGGGAT GTGGTGGTAG GCCAGCGTCC ACTGCACCTG 
CTGGAAGATG CCCTCACCAA GCCAGAGCTG CGGCCCTGCC CAGCCTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735920.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591595.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X6, mRNA.

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CloneID	OIc84647
Clone ID Related Accession (Same CDS sequence)	XM_021735920.1
Accession Version	XM_021735920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CGAGAAAGCC CTGCAGACCA ACCACTTCGA GCTCAGCTTG 
CGCACTGAAG CTACGCAGGG GCTGGTGCTG TGGAGTGGCA AGGCCACCGA GCGTGCGGAC 
TACATGGCGC TAGCCATCGT GGATGGACAC CTTCAGCTGG CCTATGACCT GGGCTCTCAG 
CCTGTGGTGC TGCGCTCCAC CGTGAGGGTC AACACCAACC GCTGGTTGCG AGTCAGGGCT 
CACAGGGAGC AGAGAGAAGG TTCCCTCCAG GTGGGCAATG AGGCCCCCGT GACTGGCTCC 
TCCCCACTGG GTGCCACACA GCTGGACACA GATGGAACTC TCTGGCTCGG GGGCCTGCAG 
AAGCTGCCTG TGGGCCAGGC CCTGCCAAAG GCCTACGGAA CAGGTTTTGT GGGCTGCCTC 
CGGGATGTGG TGGTAGGCCA GCGTCCACTG CACCTGCTGG AAGATGCCCT CACCAAGCCA 
GAGCTGCGGC CCTGCCCAGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591595.1
CDS	235..6081
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGSVGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735920.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735920.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG AAATCCCGCA AGGTGCCGCA TACCATGCTC 
AATTTGAAGG AGCCACTCTA CATCGGGGGT GCCCCCGACT TCAGCAAGCT GGCCCGGGCA 
GCTGCAGCAT CCTCTGGCTT CGACGGTGCC GTTCAGCTGG TCTCCTTGGG AGGCCGCCAG 
CTGTTGGCCC AGGAGCACGT GGTGCAGGCG GTGGACGTCT CATCCTTTAC AGACCACCCT 
TGTACCCAGG CCTCAGGCCA CCCCTGCCTC CATGGCGCCT TGTGTCTCCC AAAGGAAGCC 
ACCTACACAT GCCTGTGCCC CATGGGCTTC TCGGGGCCAC ACTGCGAGAA GGGGCTGGTT 
GAGAAGTCAG CAGGGGACCT CGAGGCTCTG GCCTTCGATG GGAGGACCTA CATCGAGTAC 
CTCAACGCTG TGACCGAGAG CGAGAAAGCC CTGCAGACCA ACCACTTCGA GCTCAGCTTG 
CGCACTGAAG CTACGCAGGG GCTGGTGCTG TGGAGTGGCA AGGCCACCGA GCGTGCGGAC 
TACATGGCGC TAGCCATCGT GGATGGACAC CTTCAGCTGG CCTATGACCT GGGCTCTCAG 
CCTGTGGTGC TGCGCTCCAC CGTGAGGGTC AACACCAACC GCTGGTTGCG AGTCAGGGCT 
CACAGGGAGC AGAGAGAAGG TTCCCTCCAG GTGGGCAATG AGGCCCCCGT GACTGGCTCC 
TCCCCACTGG GTGCCACACA GCTGGACACA GATGGAACTC TCTGGCTCGG GGGCCTGCAG 
AAGCTGCCTG TGGGCCAGGC CCTGCCAAAG GCCTACGGAA CAGGTTTTGT GGGCTGCCTC 
CGGGATGTGG TGGTAGGCCA GCGTCCACTG CACCTGCTGG AAGATGCCCT CACCAAGCCA 
GAGCTGCGGC CCTGCCCAGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735921.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591596.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X8, mRNA.

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CloneID	OIc84648
Clone ID Related Accession (Same CDS sequence)	XM_021735921.1
Accession Version	XM_021735921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG GTGCCGCATA CCATGCTCAA TTTGAAGGAG 
CCACTCTACA TCGGGGGTGC CCCCGACTTC AGCAAGCTGG CCCGGGCAGC TGCAGCATCC 
TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG 
GAGCACGTGG TGCAGGCGGT GGACGTCTCA TCCTTTACAG ACCACCCTTG TACCCAGGCC 
TCAGGCCACC CCTGCCTCCA TGGCGCCTTG TGTCTCCCAA AGGAAGCCAC CTACACATGC 
CTGTGCCCCA TGGGCTTCTC GGGGCCACAC TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA 
GGGGACCTCG AGGCTCTGGC CTTCGATGGG AGGACCTACA TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AGAAAGCCCT GCAGACCAAC CACTTCGAGC TCAGCTTGCG CACTGAAGCT 
ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG GCCACCGAGC GTGCGGACTA CATGGCGCTA 
GCCATCGTGG ATGGACACCT TCAGCTGGCC TATGACCTGG GCTCTCAGCC TGTGGTGCTG 
CGCTCCACCG TGAGGGTCAA CACCAACCGC TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG 
AGAGAAGGTT CCCTCCAGGT GGGCAATGAG GCCCCCGTGA CTGGCTCCTC CCCACTGGGT 
GCCACACAGC TGGACACAGA TGGAACTCTC TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCAAAGGC CTACGGAACA GGTTTTGTGG GCTGCCTCCG GGATGTGGTG 
GTAGGCCAGC GTCCACTGCA CCTGCTGGAA GATGCCCTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591596.1
CDS	235..6069
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGSVGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735921.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735921.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGTCAGT AGGGGAGAAG 
ATGGCCCTCG AGGTGGTGTT CTTGGCCCGA GGACCCAGCG GTTTGCTCCT GTACAATGGG 
CAAAAGACAG ATGGCAAAGG GGACTTCGTG TCACTGGCCC TGCATGACCA GCACCTGGAA 
TTCCGCTATG ACCTGGGCAA AGGGGCAGCG GTCATCAGGA GCAAGGAACC CATAGCCCTG 
GGCTCTTGGA TTAGGGTCTC GGTGGAGAGA AACGGCCGTA AGGGCGCCCT GCGTGTGGGT 
GATGGGCCCC GCGTGCTGGG AGAGTCCCCG GTGCCGCATA CCATGCTCAA TTTGAAGGAG 
CCACTCTACA TCGGGGGTGC CCCCGACTTC AGCAAGCTGG CCCGGGCAGC TGCAGCATCC 
TCTGGCTTCG ACGGTGCCGT TCAGCTGGTC TCCTTGGGAG GCCGCCAGCT GTTGGCCCAG 
GAGCACGTGG TGCAGGCGGT GGACGTCTCA TCCTTTACAG ACCACCCTTG TACCCAGGCC 
TCAGGCCACC CCTGCCTCCA TGGCGCCTTG TGTCTCCCAA AGGAAGCCAC CTACACATGC 
CTGTGCCCCA TGGGCTTCTC GGGGCCACAC TGCGAGAAGG GGCTGGTTGA GAAGTCAGCA 
GGGGACCTCG AGGCTCTGGC CTTCGATGGG AGGACCTACA TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AGAAAGCCCT GCAGACCAAC CACTTCGAGC TCAGCTTGCG CACTGAAGCT 
ACGCAGGGGC TGGTGCTGTG GAGTGGCAAG GCCACCGAGC GTGCGGACTA CATGGCGCTA 
GCCATCGTGG ATGGACACCT TCAGCTGGCC TATGACCTGG GCTCTCAGCC TGTGGTGCTG 
CGCTCCACCG TGAGGGTCAA CACCAACCGC TGGTTGCGAG TCAGGGCTCA CAGGGAGCAG 
AGAGAAGGTT CCCTCCAGGT GGGCAATGAG GCCCCCGTGA CTGGCTCCTC CCCACTGGGT 
GCCACACAGC TGGACACAGA TGGAACTCTC TGGCTCGGGG GCCTGCAGAA GCTGCCTGTG 
GGCCAGGCCC TGCCAAAGGC CTACGGAACA GGTTTTGTGG GCTGCCTCCG GGATGTGGTG 
GTAGGCCAGC GTCCACTGCA CCTGCTGGAA GATGCCCTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCAGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agrn ( XM_021735922.1 ) cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel) -> XP_021591597.1 Ictidomys tridecemlineatus agrin (Agrn), transcript variant X9, mRNA.

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CloneID	OIc84649
Clone ID Related Accession (Same CDS sequence)	XM_021735922.1
Accession Version	XM_021735922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498924800000
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Product	agrin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004936737.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictidomys tridecemlineatus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GGTGCCGCAT ACCATGCTCA ATTTGAAGGA GCCACTCTAC 
ATCGGGGGTG CCCCCGACTT CAGCAAGCTG GCCCGGGCAG CTGCAGCATC CTCTGGCTTC 
GACGGTGCCG TTCAGCTGGT CTCCTTGGGA GGCCGCCAGC TGTTGGCCCA GGAGCACGTG 
GTGCAGGCGG TGGACGTCTC ATCCTTTACA GACCACCCTT GTACCCAGGC CTCAGGCCAC 
CCCTGCCTCC ATGGCGCCTT GTGTCTCCCA AAGGAAGCCA CCTACACATG CCTGTGCCCC 
ATGGGCTTCT CGGGGCCACA CTGCGAGAAG GGGCTGGTTG AGAAGTCAGC AGGGGACCTC 
GAGGCTCTGG CCTTCGATGG GAGGACCTAC ATCGAGTACC TCAACGCTGT GACCGAGAGC 
GAGAAAGCCC TGCAGACCAA CCACTTCGAG CTCAGCTTGC GCACTGAAGC TACGCAGGGG 
CTGGTGCTGT GGAGTGGCAA GGCCACCGAG CGTGCGGACT ACATGGCGCT AGCCATCGTG 
GATGGACACC TTCAGCTGGC CTATGACCTG GGCTCTCAGC CTGTGGTGCT GCGCTCCACC 
GTGAGGGTCA ACACCAACCG CTGGTTGCGA GTCAGGGCTC ACAGGGAGCA GAGAGAAGGT 
TCCCTCCAGG TGGGCAATGA GGCCCCCGTG ACTGGCTCCT CCCCACTGGG TGCCACACAG 
CTGGACACAG ATGGAACTCT CTGGCTCGGG GGCCTGCAGA AGCTGCCTGT GGGCCAGGCC 
CTGCCAAAGG CCTACGGAAC AGGTTTTGTG GGCTGCCTCC GGGATGTGGT GGTAGGCCAG 
CGTCCACTGC ACCTGCTGGA AGATGCCCTC ACCAAGCCAG AGCTGCGGCC CTGCCCAGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021591597.1
CDS	234..6059
Translation	MPPLPLERPPRQQPGASLVVRYFMIPCNVCLILLATSTLGFAVLLFLNNYKPGTHFTPAPPMPPDVCRGMLCGFGAVCEPSAEDPGRASCVCKKSACPAVVAPVCGSDASTYSNECELQRAQCRLQRRIRLLRRGPCGSRDPCSNVTCSFGSTCAPSADGQTASCLCPTTCRGAPEGTVCGSDGMDYPGECQLLRRACARQENIFRKFDGPCDPCQGAHADPSRVCRVNPRTRRPEMLLRPESCPPRHLPVCGDDGVTYANDCVMGRSGAVRGLLLQKVRSGQCQPRDQCSGTCQFSAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQRAIPPRHQGPCDQAPSPCRGVQCAFGATCAVKNGEAACECQQACAGAYDPVCGSDGVTYGSTCELEAMACALGRDIRVARRGPCDRCGQCRFGALCEAETGRCVCPSECVASAQPVCGSDGHTYASECELHVHACTHQISLHVASAGHCQTCGDTVCAFGAVCSAGQCVCPRCERPPPGPVCGSDGVTYRSACELREAACQQQIQLEEARAGPCEQAECGSGGSGSGEDSECEQELCRQRGGVWDEDSEDGPCVCDFSCQGVLRSLVCGSDGVTYGSECDLKRARCESQGELYVAAQGPCRGLTLAPLLPAAPAQCAQTPYGCCQDNVTAARGVGLAGCPSTCQCNPHGSYGGTCDPATGQCSCRPGVGGLKCDRCEPGFWNFRGIVTDGRSGCTPCSCDPRGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGHTLGPTGCEADPSAPVTCAEMHCEFGASCVEEAGSAHCVCPALTCPEASATKVCGSDGITYGSECQLKTIACRQGLDIFTQSLGPCQEAVVPGAHPTSRATTAPGRLPSEPLLPPPSALPLAPSSTPQSRPTPPPTLRPRTTASLPRSTARPALTMPPIETSTATSLSASAFGESGSTDGSGDEELSGDQEASGGGSGGLELSMDSSVVTQGPPMERASCYNSPLGCCSDGQTPSLDAEGSNCPATKVYQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDALFRNSDVKKDFRSVHLRDLGPGRSVRATVDVHFDPTTAFRAPDVGRALLRQIQASRRRALAVRRPLQEHVRFMDFDWFPAFLTGATTGATAAVATARATTVGPPPSSVTPRVPPSHTSRPTGRTTAPTHTHRPPTTAPGHVAGRWPLLPNPKQHPRSCDSQPCLHGGTCQDQDSGRGFTCSCPAGRGGTFCEDVLRPSVSVPAFGGASFLAFPTLRAYHTLRLALEFRSLEPQGLLLYNGNARGKDFLALALLGGRVQLRFDTGSGPAILTSSVPVEPGRWHRLELSRHWRQGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQADVAHERTSVGVGLKGCIRLLDVNNQRLELGPWQGVVSRSSGVGECGEHPCMPSPCHGGAPCQALGDDMFRCQCPPGRFGPTCAEEKSPCQPNPCHGAAPCRVLPKGGAKCECPPGRRGTLCQTVSEMDGSAQPFLADFHGFSYLELRGLHTFERDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALHDQHLEFRYDLGKGAAVIRSKEPIALGSWIRVSVERNGRKGALRVGDGPRVLGESPVPHTMLNLKEPLYIGGAPDFSKLARAAAASSGFDGAVQLVSLGGRQLLAQEHVVQAVDVSSFTDHPCTQASGHPCLHGALCLPKEATYTCLCPMGFSGPHCEKGLVEKSAGDLEALAFDGRTYIEYLNAVTESEKALQTNHFELSLRTEATQGLVLWSGKATERADYMALAIVDGHLQLAYDLGSQPVVLRSTVRVNTNRWLRVRAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGTLWLGGLQKLPVGQALPKAYGTGFVGCLRDVVVGQRPLHLLEDALTKPELRPCPAS

Target ORF information:

RefSeq Version	XM_021735922.1
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)
Definition	Ictidomys tridecemlineatus agrin (Agrn), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021735922.1

ORF Insert Sequence:

ATGCCTCCGT TGCCGCTGGA GCGGCCCCCC CGGCAGCAGC CTGGCGCCTC GCTGGTGGTG 
CGGTACTTCA TGATCCCCTG CAACGTCTGT CTGATCCTGC TGGCCACCTC TACGCTGGGC 
TTCGCGGTGC TGCTCTTCCT CAACAACTAC AAACCTGGGA CCCACTTCAC ACCAGCACCT 
CCCATGCCTC CTGACGTCTG CAGGGGAATG CTGTGTGGCT TTGGCGCGGT CTGTGAGCCC 
AGTGCAGAGG ATCCAGGCCG GGCCTCCTGC GTGTGCAAGA AGAGCGCCTG TCCTGCCGTC 
GTGGCGCCAG TGTGCGGCTC AGATGCCTCC ACCTACAGCA ACGAGTGCGA GCTGCAGCGT 
GCACAGTGCC GCCTGCAGCG GCGAATCCGC CTGCTCCGCC GCGGACCCTG TGGTTCCCGG 
GACCCCTGCT CCAATGTGAC CTGCAGCTTC GGCAGCACCT GTGCCCCCTC CGCCGATGGG 
CAGACCGCCT CCTGCCTGTG TCCCACGACC TGCCGCGGGG CCCCGGAGGG CACCGTGTGT 
GGCAGTGATG GTATGGACTA CCCCGGTGAG TGCCAGCTGC TGCGCCGGGC ATGCGCACGC 
CAGGAGAACA TCTTTAGGAA GTTCGACGGC CCCTGTGACC CCTGCCAGGG AGCCCATGCT 
GACCCGAGCC GCGTCTGCCG TGTGAACCCG CGCACTCGCC GTCCAGAGAT GCTCCTGCGC 
CCAGAGAGCT GCCCTCCCCG GCATTTGCCT GTGTGTGGGG ATGATGGGGT CACCTATGCA 
AATGACTGCG TCATGGGCCG CTCAGGGGCT GTCCGCGGTC TGCTCCTCCA GAAAGTGCGC 
TCTGGCCAGT GCCAGCCTCG AGACCAGTGC TCCGGGACCT GCCAGTTCAG TGCCGTTTGC 
CTGTCCCGCC GTGGCCGACC CCGCTGCTCC TGCGACCGTG TCACCTGTGA TGGGGCCTAC 
AGACCGGTGT GTGCCCAGGA TGGGCACACG TATGACAACG ACTGTTGGCG CCAGCAGGCT 
GAGTGCCGGC AGCAGCGGGC CATCCCTCCC AGGCACCAGG GCCCGTGTGA CCAGGCTCCG 
TCCCCGTGCC GGGGGGTGCA GTGCGCATTC GGGGCGACAT GCGCCGTGAA GAACGGGGAG 
GCTGCGTGCG AGTGCCAGCA GGCGTGTGCA GGCGCCTACG ACCCCGTGTG CGGCAGTGAC 
GGCGTCACCT ATGGCAGCAC CTGCGAGCTG GAGGCCATGG CCTGTGCCCT TGGGCGGGAT 
ATCCGGGTGG CCCGCAGAGG ACCCTGTGAC CGATGCGGGC AGTGCCGTTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG TTGTGTGTGC CCCTCTGAGT GCGTGGCCTC AGCCCAGCCC 
GTGTGCGGTT CTGATGGGCA CACGTATGCC AGCGAGTGTG AGCTGCATGT CCACGCCTGC 
ACACACCAGA TAAGCCTGCA TGTGGCATCA GCTGGACACT GTCAGACCTG TGGAGACACA 
GTGTGTGCCT TTGGGGCTGT GTGCTCAGCC GGGCAGTGTG TGTGTCCTCG TTGTGAGCGC 
CCCCCGCCTG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACCGCAGTGC GTGTGAGCTA 
CGGGAGGCTG CCTGCCAGCA GCAGATACAG CTTGAGGAAG CCCGGGCAGG GCCCTGTGAG 
CAGGCTGAGT GTGGCTCAGG AGGCTCCGGC TCTGGGGAGG ACAGCGAGTG TGAGCAGGAA 
CTCTGCCGGC AGCGAGGCGG GGTGTGGGAT GAGGACTCAG AGGATGGACC CTGCGTCTGC 
GACTTCAGCT GCCAGGGTGT TCTCAGGAGC CTGGTGTGCG GCTCAGATGG GGTCACCTAT 
GGCAGTGAGT GTGACCTGAA GAGGGCCAGG TGCGAGTCTC AAGGAGAGTT GTACGTCGCA 
GCTCAGGGAC CCTGCCGAGG CCTCACCTTG GCCCCACTGC TGCCTGCAGC CCCCGCTCAA 
TGTGCCCAGA CCCCCTACGG ATGCTGCCAG GATAATGTCA CTGCTGCCCG GGGTGTGGGC 
CTGGCCGGTT GCCCCAGCAC CTGCCAGTGC AACCCTCACG GCTCCTACGG AGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCTTGCCGG CCAGGTGTGG GCGGCCTCAA GTGTGACCGA 
TGTGAGCCTG GTTTCTGGAA CTTCCGTGGC ATTGTCACTG ACGGCCGGAG CGGCTGTACT 
CCCTGCAGCT GTGACCCCCG GGGTGCCGTG CGGGATGACT GTGAGCAGAT GACTGGGCTG 
TGCTCCTGTA AGCCGGGCGT CGCTGGTCCC AAGTGCGGGC AGTGTCCTGA TGGCCACACC 
CTGGGCCCCA CCGGCTGTGA GGCAGATCCT TCAGCACCGG TGACCTGTGC GGAGATGCAC 
TGTGAGTTTG GCGCTTCCTG CGTGGAAGAG GCAGGTTCTG CCCACTGCGT CTGCCCAGCC 
CTCACCTGCC CAGAGGCCAG TGCCACCAAG GTCTGTGGAT CAGATGGCAT CACCTACGGC 
AGCGAGTGCC AACTGAAGAC CATCGCCTGC CGCCAGGGCT TGGACATCTT CACCCAGAGC 
CTCGGCCCGT GCCAGGAGGC TGTGGTTCCT GGCGCTCACC CGACCTCCAG GGCCACGACG 
GCCCCGGGGC GCTTGCCGAG CGAGCCTCTG CTGCCTCCAC CCAGTGCCCT CCCCCTGGCT 
CCCAGCAGTA CCCCCCAGAG CCGGCCCACC CCTCCACCCA CCTTACGACC TCGGACCACA 
GCCAGCCTCC CCAGGAGCAC AGCTCGGCCG GCGCTGACCA TGCCTCCCAT AGAGACCTCC 
ACTGCAACCA GTCTCTCCGC CTCTGCCTTT GGAGAGTCTG GCAGCACTGA TGGGAGTGGC 
GATGAGGAAC TGAGTGGGGA CCAGGAGGCC AGTGGGGGTG GTTCTGGGGG ACTGGAACTC 
TCCATGGACA GCAGCGTGGT GACACAGGGA CCACCCATGG AGAGAGCCTC CTGCTACAAC 
TCACCTTTGG GCTGCTGTTC GGATGGCCAG ACACCTTCGC TGGACGCAGA GGGCTCCAAC 
TGTCCTGCCA CCAAGGTGTA CCAGGGCGTG CTGGAGCTGG AGGGGGTCGA GGGGCAAGAG 
CTGTTCTACA CGCCCGAAAT GGCCGACCCC AAGTCAGAGC TGTTTGGGGA GACGGCCAGG 
AGCATCGAGA GCACTCTGGA CGCCCTTTTC CGGAACTCAG ACGTCAAAAA GGACTTCCGG 
AGTGTCCACC TGCGGGACCT GGGGCCTGGC AGATCAGTCC GTGCCACTGT GGATGTGCAC 
TTTGACCCCA CCACAGCCTT CAGGGCACCT GACGTGGGCC GGGCCCTGCT GCGCCAGATC 
CAGGCCTCCA GGCGCCGGGC CCTGGCGGTG CGGAGGCCTC TGCAGGAGCA CGTGCGGTTC 
ATGGACTTCG ACTGGTTTCC TGCGTTCCTC ACGGGAGCCA CCACAGGAGC CACCGCTGCA 
GTGGCCACGG CCAGAGCTAC CACTGTGGGC CCACCACCTT CCTCCGTGAC CCCTCGGGTC 
CCCCCCAGTC ACACCAGCAG GCCCACTGGA AGGACCACAG CACCCACACA CACACACCGG 
CCCCCCACCA CAGCTCCCGG CCATGTGGCT GGACGCTGGC CTTTGCTCCC CAATCCCAAG 
CAGCACCCCA GGTCCTGTGA TTCTCAGCCC TGCCTGCATG GGGGGACCTG CCAGGACCAG 
GACTCTGGCA GGGGCTTCAC CTGCAGCTGC CCGGCAGGCC GGGGAGGCAC CTTCTGTGAG 
GACGTGCTGC GCCCCTCTGT CTCAGTTCCT GCGTTTGGGG GTGCCTCCTT TCTGGCCTTC 
CCCACCCTCC GGGCATATCA CACGCTGCGC CTGGCGCTGG AATTCCGATC CCTGGAGCCT 
CAGGGACTGC TGCTGTACAA CGGCAACGCC CGTGGCAAGG ACTTCCTGGC CCTGGCGCTG 
CTCGGTGGGC GCGTGCAGCT CAGGTTTGAC ACAGGTTCTG GACCAGCAAT ACTGACCAGC 
TCGGTGCCTG TGGAACCAGG GCGGTGGCAT CGCCTAGAAC TCTCACGGCA CTGGCGCCAA 
GGCACACTGT CTGTGGATGG CGAGACCCCA GTTCTGGGCG AGAGCCCCAG TGGCACGGAT 
GGCCTCAACC TAGATACCGA CCTCTTTGTG GGCGGAGTCC CAGAGGACCA GGCTGACGTG 
GCCCATGAGC GGACCTCGGT TGGCGTGGGC CTGAAGGGCT GCATCCGCCT GCTGGATGTC 
AACAATCAGC GCCTGGAGCT GGGCCCCTGG CAGGGCGTGG TGTCCCGCAG CTCCGGGGTG 
GGAGAGTGTG GAGAGCACCC CTGCATGCCC AGCCCCTGCC ATGGCGGGGC ACCATGCCAG 
GCCCTGGGAG ACGACATGTT TCGCTGCCAG TGTCCACCCG GCCGCTTTGG CCCAACCTGT 
GCGGAAGAGA AGAGCCCCTG CCAGCCGAAC CCCTGCCACG GTGCAGCCCC CTGCCGCGTG 
CTACCCAAGG GCGGGGCCAA GTGCGAGTGC CCCCCGGGAC GTAGAGGCAC TCTCTGCCAG 
ACAGTGTCTG AGATGGATGG TAGTGCCCAG CCCTTCCTGG CGGACTTCCA TGGCTTTTCC 
TACCTGGAGC TGAGAGGCTT GCACACCTTT GAGAGAGACC TGGGGGAGAA GATGGCCCTC 
GAGGTGGTGT TCTTGGCCCG AGGACCCAGC GGTTTGCTCC TGTACAATGG GCAAAAGACA 
GATGGCAAAG GGGACTTCGT GTCACTGGCC CTGCATGACC AGCACCTGGA ATTCCGCTAT 
GACCTGGGCA AAGGGGCAGC GGTCATCAGG AGCAAGGAAC CCATAGCCCT GGGCTCTTGG 
ATTAGGGTCT CGGTGGAGAG AAACGGCCGT AAGGGCGCCC TGCGTGTGGG TGATGGGCCC 
CGCGTGCTGG GAGAGTCCCC GGTGCCGCAT ACCATGCTCA ATTTGAAGGA GCCACTCTAC 
ATCGGGGGTG CCCCCGACTT CAGCAAGCTG GCCCGGGCAG CTGCAGCATC CTCTGGCTTC 
GACGGTGCCG TTCAGCTGGT CTCCTTGGGA GGCCGCCAGC TGTTGGCCCA GGAGCACGTG 
GTGCAGGCGG TGGACGTCTC ATCCTTTACA GACCACCCTT GTACCCAGGC CTCAGGCCAC 
CCCTGCCTCC ATGGCGCCTT GTGTCTCCCA AAGGAAGCCA CCTACACATG CCTGTGCCCC 
ATGGGCTTCT CGGGGCCACA CTGCGAGAAG GGGCTGGTTG AGAAGTCAGC AGGGGACCTC 
GAGGCTCTGG CCTTCGATGG GAGGACCTAC ATCGAGTACC TCAACGCTGT GACCGAGAGC 
GAGAAAGCCC TGCAGACCAA CCACTTCGAG CTCAGCTTGC GCACTGAAGC TACGCAGGGG 
CTGGTGCTGT GGAGTGGCAA GGCCACCGAG CGTGCGGACT ACATGGCGCT AGCCATCGTG 
GATGGACACC TTCAGCTGGC CTATGACCTG GGCTCTCAGC CTGTGGTGCT GCGCTCCACC 
GTGAGGGTCA ACACCAACCG CTGGTTGCGA GTCAGGGCTC ACAGGGAGCA GAGAGAAGGT 
TCCCTCCAGG TGGGCAATGA GGCCCCCGTG ACTGGCTCCT CCCCACTGGG TGCCACACAG 
CTGGACACAG ATGGAACTCT CTGGCTCGGG GGCCTGCAGA AGCTGCCTGT GGGCCAGGCC 
CTGCCAAAGG CCTACGGAAC AGGTTTTGTG GGCTGCCTCC GGGATGTGGT GGTAGGCCAG 
CGTCCACTGC ACCTGCTGGA AGATGCCCTC ACCAAGCCAG AGCTGCGGCC CTGCCCAGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Agrn
Entrez Gene ID	101956812
Full Name	agrin
Gene Type	protein-coding
Organism	Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Agrn cDNA ORF clone, Ictidomys tridecemlineatus(thirteen-lined ground squirrel)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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