NOS1 cDNA ORF clone, Felis catus(domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS1
Entrez Gene ID	101100342
Full Name	nitric oxide synthase 1
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011287802.2	XP_011286104.1	nitric oxide synthase, brain isoform X1
XM_011287802.3	XP_011286104.1	nitric oxide synthase, brain isoform X1
XM_019814593.1	XP_019670152.1	nitric oxide synthase, brain isoform X1
XM_023241468.1	XP_023097236.1	nitric oxide synthase, brain isoform X1
XM_011287803.2	XP_011286105.1	nitric oxide synthase, brain isoform X2
XM_011287803.3	XP_011286105.1	nitric oxide synthase, brain isoform X2

Pathways from BioSystems
Gene Ontology

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb34714	XM_023241468.1 Latest version!	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb34714	XM_011287802.3 Latest version!	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb34714	XM_019814593.1 Latest version!	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb34715	XM_011287803.3 Latest version!	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OFb34714	XM_011287802.2	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OFb34715	XM_011287803.2	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS1 ( XM_011287802.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_011286104.1 Felis catus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

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CloneID	OFb34714
Clone ID Related Accession (Same CDS sequence)	XM_011287802.2 , XM_011287802.3 , XM_019814593.1 , XM_023241468.1
Accession Version	XM_011287802.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	nitric oxide synthase, brain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_011287802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011286104.1
CDS	725..5116
Translation	MENQVFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTTAVDLSHQSSASREHSPAVDRATGPGNGPQHAQDAGQEAGSLPHTKSRAPGPPGQDSAKKSIQGSQNSDLLKEIEPVLSLLTSGSKGINGGGPSKAETKDAEVQVDRGMDGKSHKPLPLGVENDRVFSDLWGKGHVPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLTDTLHLKSTLEMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFPLAKEFIDQYYSSIKRFGSKAHTERLEEVNKEIETTGTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVEFTEICVQQGWKPPRGRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPRFEWFKDLGLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKNMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSVTPVFHQEMLNYRLTPSFEYQPEPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPQGDTEVHGLAAARDSQQRSYKVRFNSVSSYSDSHKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNREDLVNALMERLEDAPPVNQLVKVELLVERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPTTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYHTRDGEGPIHHGVCSSWLNRIPADEVVPCFVRGAPSFHLPRNPQVPCILIGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEHLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_011287802.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011287802.2

ORF Insert Sequence:

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_011287802.3 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_011286104.1 Felis catus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb34714
Clone ID Related Accession (Same CDS sequence)	XM_011287802.2 , XM_011287802.3 , XM_019814593.1 , XM_023241468.1
Accession Version	XM_011287802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_011287802.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011286104.1
CDS	724..5115
Translation	MENQVFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTTAVDLSHQSSASREHSPAVDRATGPGNGPQHAQDAGQEAGSLPHTKSRAPGPPGQDSAKKSIQGSQNSDLLKEIEPVLSLLTSGSKGINGGGPSKAETKDAEVQVDRGMDGKSHKPLPLGVENDRVFSDLWGKGHVPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLTDTLHLKSTLEMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFPLAKEFIDQYYSSIKRFGSKAHTERLEEVNKEIETTGTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVEFTEICVQQGWKPPRGRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPRFEWFKDLGLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKNMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSVTPVFHQEMLNYRLTPSFEYQPEPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPQGDTEVHGLAAARDSQQRSYKVRFNSVSSYSDSHKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNREDLVNALMERLEDAPPVNQLVKVELLVERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPTTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYHTRDGEGPIHHGVCSSWLNRIPADEVVPCFVRGAPSFHLPRNPQVPCILIGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEHLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_011287802.3
Organism	Felis catus(domestic cat)
Definition	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011287802.3

ORF Insert Sequence:

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_019814593.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_019670152.1 Felis catus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

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CloneID	OFb34714
Clone ID Related Accession (Same CDS sequence)	XM_011287802.2 , XM_011287802.3 , XM_019814593.1 , XM_023241468.1
Accession Version	XM_019814593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	nitric oxide synthase, brain isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019670152.1
CDS	440..4831
Translation	MENQVFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTTAVDLSHQSSASREHSPAVDRATGPGNGPQHAQDAGQEAGSLPHTKSRAPGPPGQDSAKKSIQGSQNSDLLKEIEPVLSLLTSGSKGINGGGPSKAETKDAEVQVDRGMDGKSHKPLPLGVENDRVFSDLWGKGHVPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLTDTLHLKSTLEMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFPLAKEFIDQYYSSIKRFGSKAHTERLEEVNKEIETTGTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVEFTEICVQQGWKPPRGRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPRFEWFKDLGLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKNMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSVTPVFHQEMLNYRLTPSFEYQPEPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPQGDTEVHGLAAARDSQQRSYKVRFNSVSSYSDSHKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNREDLVNALMERLEDAPPVNQLVKVELLVERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPTTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYHTRDGEGPIHHGVCSSWLNRIPADEVVPCFVRGAPSFHLPRNPQVPCILIGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEHLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_019814593.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019814593.1

ORF Insert Sequence:

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_023241468.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023097236.1 Felis catus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

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CloneID	OFb34714
Clone ID Related Accession (Same CDS sequence)	XM_011287802.2 , XM_011287802.3 , XM_019814593.1 , XM_023241468.1
Accession Version	XM_023241468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	nitric oxide synthase, brain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023097236.1
CDS	468..4859
Translation	MENQVFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTTAVDLSHQSSASREHSPAVDRATGPGNGPQHAQDAGQEAGSLPHTKSRAPGPPGQDSAKKSIQGSQNSDLLKEIEPVLSLLTSGSKGINGGGPSKAETKDAEVQVDRGMDGKSHKPLPLGVENDRVFSDLWGKGHVPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLTDTLHLKSTLEMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFPLAKEFIDQYYSSIKRFGSKAHTERLEEVNKEIETTGTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVEFTEICVQQGWKPPRGRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPRFEWFKDLGLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKNMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSVTPVFHQEMLNYRLTPSFEYQPEPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKYPEPLRFFPRKGPPLPQGDTEVHGLAAARDSQQRSYKVRFNSVSSYSDSHKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNREDLVNALMERLEDAPPVNQLVKVELLVERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPTTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYHTRDGEGPIHHGVCSSWLNRIPADEVVPCFVRGAPSFHLPRNPQVPCILIGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEHLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_023241468.1
Organism	Felis catus(domestic cat)
Definition	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023241468.1

ORF Insert Sequence:

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGTAC 
CCGGAACCCT TGCGTTTCTT TCCCCGTAAA GGGCCTCCCC TCCCCCAAGG TGACACAGAA 
GTCCATGGTC TGGCTGCAGC CCGTGACAGC CAGCAGAGGA GCTACAAGGT CCGATTCAAT 
AGTGTTTCCT CGTATTCTGA CTCCCATAAA TCATCAGGCG ACGGGCCAGA CCTCAGAGAC 
AACTTTGAGA GTGCTGGACC CCTGGCCAAC GTGAGGTTCT CCGTATTTGG CCTTGGCTCC 
CGGGCTTACC CTCATTTCTG CGCCTTTGGA CATGCCGTGG ACACCCTCCT GGAGGAGCTG 
GGAGGAGAGA GGATCCTGAA GATGAGGGAG GGAGACGAGC TCTGTGGGCA GGAAGAGGCT 
TTCAGGACCT GGGCCAAGAA GGTCTTCAAG GCAGCCTGCG ATGTGTTCTG CGTGGGAGAT 
GACGTCAACA TTGAGAAGGC AAACAACTCC CTCATCAGCA ACGACCGCAG CTGGAAAAGA 
AACAAGTTCC GCCTCACCTA CGTGGCCGAA GCTCCGGAAC TTACACAAGG TCTATCCAAT 
GTCCACAAAA AGCGGGTCTC GGCCGCTCGA CTCCTCAGCC GCCAAAACCT TCAGAGCCCT 
AAATCCAGCC GATCAACGAT CTTCGTGCGT CTCCACACCA ATGGGAATCA GGAGCTGCAG 
TACCAGCCTG GGGACCACCT GGGCGTCTTC CCTGGCAACC GCGAGGACCT CGTGAACGCC 
CTGATGGAGC GGCTGGAAGA TGCTCCCCCA GTCAACCAGC TGGTGAAGGT GGAACTGCTG 
GTGGAGAGGA ACACGGCTTT GGGCGTCATC AGTAACTGGA CAGATGAGCA CCGCCTCCCG 
CCCTGCACCA TCTTCCAGGC CTTCAAGTAC TACCTGGACA TCACCACACC GCCCACGCCA 
CTGCAGCTGC AGCAGTTTGC GTCCCTGGCC ACCAGCGAGA AAGAGAAGCA GCGACTGCTG 
GTCCTCAGCA AGGGCTTGCA GGAGTACGAG GAATGGAAAT GGGGCAAGAA CCCCACCATC 
GTGGAGGTGC TGGAAGAGTT CCCGTCCATC CAGATGCCCA CCACCCTGCT CCTGACCCAG 
CTGTCCCTGC TGCAGCCTCG ATACTATTCA ATCAGTTCTT CCCCAGACAT GTACCCTGAC 
GAGGTGCACC TCACCGTGGC CGTTGTCTCC TACCACACCC GAGATGGAGA AGGACCAATT 
CACCATGGCG TGTGCTCCTC CTGGCTCAAC CGGATACCGG CTGATGAAGT GGTCCCCTGT 
TTCGTGAGAG GAGCACCCAG CTTCCACCTG CCCCGAAATC CCCAAGTGCC CTGTATCCTC 
ATTGGCCCAG GCACTGGCAT TGCCCCCTTC CGAAGCTTCT GGCAGCAGCG GCAATTTGAT 
ATCCAGCACA AAGGAATGAG CCCCTGCCCC ATGGTCCTGG TGTTTGGGTG CCGGCAATCC 
AAGATAGACC ACATCTACAG GGAGGAAACT CTACAGGCCA AGAACAAGGG GGTCTTCAGA 
GAACTCTACA CGGCCTACTC GCGGGAGCCA GACAAACCAA AGAAGTACGT TCAGGACATC 
CTGCAGGAGC ACTTGGCAGA ACCTGTGTAC CGAGCCCTGA AGGAGCAAGG GGGCCACATT 
TACGTCTGCG GGGACGTCAC CATGGCCGCC GATGTCCTCA AAGCCATCCA GCGCATCATG 
ACCCAGCAAG GGAAACTCTC AGCGGAGGAT GCCGGCGTGT TCATCAGCAG GCTGAGGGAT 
GACAACCGGT ACCATGAGGA TATTTTCGGG GTCACCCTGC GAACATATGA AGTGACCAAC 
CGCCTTAGAT CTGAATCCAT TGCCTTCATT GAGGAGAGCA AAAAAGACAC TGATGAAGTT 
TTCAGCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_011287803.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_011286105.1 Felis catus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OFb34715
Clone ID Related Accession (Same CDS sequence)	XM_011287803.2 , XM_011287803.3
Accession Version	XM_011287803.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	nitric oxide synthase, brain isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_011287803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGAGC 
TACAAGGTCC GATTCAATAG TGTTTCCTCG TATTCTGACT CCCATAAATC ATCAGGCGAC 
GGGCCAGACC TCAGAGACAA CTTTGAGAGT GCTGGACCCC TGGCCAACGT GAGGTTCTCC 
GTATTTGGCC TTGGCTCCCG GGCTTACCCT CATTTCTGCG CCTTTGGACA TGCCGTGGAC 
ACCCTCCTGG AGGAGCTGGG AGGAGAGAGG ATCCTGAAGA TGAGGGAGGG AGACGAGCTC 
TGTGGGCAGG AAGAGGCTTT CAGGACCTGG GCCAAGAAGG TCTTCAAGGC AGCCTGCGAT 
GTGTTCTGCG TGGGAGATGA CGTCAACATT GAGAAGGCAA ACAACTCCCT CATCAGCAAC 
GACCGCAGCT GGAAAAGAAA CAAGTTCCGC CTCACCTACG TGGCCGAAGC TCCGGAACTT 
ACACAAGGTC TATCCAATGT CCACAAAAAG CGGGTCTCGG CCGCTCGACT CCTCAGCCGC 
CAAAACCTTC AGAGCCCTAA ATCCAGCCGA TCAACGATCT TCGTGCGTCT CCACACCAAT 
GGGAATCAGG AGCTGCAGTA CCAGCCTGGG GACCACCTGG GCGTCTTCCC TGGCAACCGC 
GAGGACCTCG TGAACGCCCT GATGGAGCGG CTGGAAGATG CTCCCCCAGT CAACCAGCTG 
GTGAAGGTGG AACTGCTGGT GGAGAGGAAC ACGGCTTTGG GCGTCATCAG TAACTGGACA 
GATGAGCACC GCCTCCCGCC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACACCGC CCACGCCACT GCAGCTGCAG CAGTTTGCGT CCCTGGCCAC CAGCGAGAAA 
GAGAAGCAGC GACTGCTGGT CCTCAGCAAG GGCTTGCAGG AGTACGAGGA ATGGAAATGG 
GGCAAGAACC CCACCATCGT GGAGGTGCTG GAAGAGTTCC CGTCCATCCA GATGCCCACC 
ACCCTGCTCC TGACCCAGCT GTCCCTGCTG CAGCCTCGAT ACTATTCAAT CAGTTCTTCC 
CCAGACATGT ACCCTGACGA GGTGCACCTC ACCGTGGCCG TTGTCTCCTA CCACACCCGA 
GATGGAGAAG GACCAATTCA CCATGGCGTG TGCTCCTCCT GGCTCAACCG GATACCGGCT 
GATGAAGTGG TCCCCTGTTT CGTGAGAGGA GCACCCAGCT TCCACCTGCC CCGAAATCCC 
CAAGTGCCCT GTATCCTCAT TGGCCCAGGC ACTGGCATTG CCCCCTTCCG AAGCTTCTGG 
CAGCAGCGGC AATTTGATAT CCAGCACAAA GGAATGAGCC CCTGCCCCAT GGTCCTGGTG 
TTTGGGTGCC GGCAATCCAA GATAGACCAC ATCTACAGGG AGGAAACTCT ACAGGCCAAG 
AACAAGGGGG TCTTCAGAGA ACTCTACACG GCCTACTCGC GGGAGCCAGA CAAACCAAAG 
AAGTACGTTC AGGACATCCT GCAGGAGCAC TTGGCAGAAC CTGTGTACCG AGCCCTGAAG 
GAGCAAGGGG GCCACATTTA CGTCTGCGGG GACGTCACCA TGGCCGCCGA TGTCCTCAAA 
GCCATCCAGC GCATCATGAC CCAGCAAGGG AAACTCTCAG CGGAGGATGC CGGCGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CATGAGGATA TTTTCGGGGT CACCCTGCGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAATCCATTG CCTTCATTGA GGAGAGCAAA 
AAAGACACTG ATGAAGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011286105.1
CDS	725..5014
Translation	MENQVFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTTAVDLSHQSSASREHSPAVDRATGPGNGPQHAQDAGQEAGSLPHTKSRAPGPPGQDSAKKSIQGSQNSDLLKEIEPVLSLLTSGSKGINGGGPSKAETKDAEVQVDRGMDGKSHKPLPLGVENDRVFSDLWGKGHVPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLTDTLHLKSTLEMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFPLAKEFIDQYYSSIKRFGSKAHTERLEEVNKEIETTGTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVEFTEICVQQGWKPPRGRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPRFEWFKDLGLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKNMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSVTPVFHQEMLNYRLTPSFEYQPEPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSHKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNREDLVNALMERLEDAPPVNQLVKVELLVERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPTTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYHTRDGEGPIHHGVCSSWLNRIPADEVVPCFVRGAPSFHLPRNPQVPCILIGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEHLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_011287803.2
Organism	Felis catus(Domestic cat)
Definition	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011287803.2

ORF Insert Sequence:

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGAGC 
TACAAGGTCC GATTCAATAG TGTTTCCTCG TATTCTGACT CCCATAAATC ATCAGGCGAC 
GGGCCAGACC TCAGAGACAA CTTTGAGAGT GCTGGACCCC TGGCCAACGT GAGGTTCTCC 
GTATTTGGCC TTGGCTCCCG GGCTTACCCT CATTTCTGCG CCTTTGGACA TGCCGTGGAC 
ACCCTCCTGG AGGAGCTGGG AGGAGAGAGG ATCCTGAAGA TGAGGGAGGG AGACGAGCTC 
TGTGGGCAGG AAGAGGCTTT CAGGACCTGG GCCAAGAAGG TCTTCAAGGC AGCCTGCGAT 
GTGTTCTGCG TGGGAGATGA CGTCAACATT GAGAAGGCAA ACAACTCCCT CATCAGCAAC 
GACCGCAGCT GGAAAAGAAA CAAGTTCCGC CTCACCTACG TGGCCGAAGC TCCGGAACTT 
ACACAAGGTC TATCCAATGT CCACAAAAAG CGGGTCTCGG CCGCTCGACT CCTCAGCCGC 
CAAAACCTTC AGAGCCCTAA ATCCAGCCGA TCAACGATCT TCGTGCGTCT CCACACCAAT 
GGGAATCAGG AGCTGCAGTA CCAGCCTGGG GACCACCTGG GCGTCTTCCC TGGCAACCGC 
GAGGACCTCG TGAACGCCCT GATGGAGCGG CTGGAAGATG CTCCCCCAGT CAACCAGCTG 
GTGAAGGTGG AACTGCTGGT GGAGAGGAAC ACGGCTTTGG GCGTCATCAG TAACTGGACA 
GATGAGCACC GCCTCCCGCC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACACCGC CCACGCCACT GCAGCTGCAG CAGTTTGCGT CCCTGGCCAC CAGCGAGAAA 
GAGAAGCAGC GACTGCTGGT CCTCAGCAAG GGCTTGCAGG AGTACGAGGA ATGGAAATGG 
GGCAAGAACC CCACCATCGT GGAGGTGCTG GAAGAGTTCC CGTCCATCCA GATGCCCACC 
ACCCTGCTCC TGACCCAGCT GTCCCTGCTG CAGCCTCGAT ACTATTCAAT CAGTTCTTCC 
CCAGACATGT ACCCTGACGA GGTGCACCTC ACCGTGGCCG TTGTCTCCTA CCACACCCGA 
GATGGAGAAG GACCAATTCA CCATGGCGTG TGCTCCTCCT GGCTCAACCG GATACCGGCT 
GATGAAGTGG TCCCCTGTTT CGTGAGAGGA GCACCCAGCT TCCACCTGCC CCGAAATCCC 
CAAGTGCCCT GTATCCTCAT TGGCCCAGGC ACTGGCATTG CCCCCTTCCG AAGCTTCTGG 
CAGCAGCGGC AATTTGATAT CCAGCACAAA GGAATGAGCC CCTGCCCCAT GGTCCTGGTG 
TTTGGGTGCC GGCAATCCAA GATAGACCAC ATCTACAGGG AGGAAACTCT ACAGGCCAAG 
AACAAGGGGG TCTTCAGAGA ACTCTACACG GCCTACTCGC GGGAGCCAGA CAAACCAAAG 
AAGTACGTTC AGGACATCCT GCAGGAGCAC TTGGCAGAAC CTGTGTACCG AGCCCTGAAG 
GAGCAAGGGG GCCACATTTA CGTCTGCGGG GACGTCACCA TGGCCGCCGA TGTCCTCAAA 
GCCATCCAGC GCATCATGAC CCAGCAAGGG AAACTCTCAG CGGAGGATGC CGGCGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CATGAGGATA TTTTCGGGGT CACCCTGCGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAATCCATTG CCTTCATTGA GGAGAGCAAA 
AAAGACACTG ATGAAGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS1 ( XM_011287803.3 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_011286105.1 Felis catus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OFb34715
Clone ID Related Accession (Same CDS sequence)	XM_011287803.2 , XM_011287803.3
Accession Version	XM_011287803.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	nitric oxide synthase, brain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018734.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_011287803.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGAGC 
TACAAGGTCC GATTCAATAG TGTTTCCTCG TATTCTGACT CCCATAAATC ATCAGGCGAC 
GGGCCAGACC TCAGAGACAA CTTTGAGAGT GCTGGACCCC TGGCCAACGT GAGGTTCTCC 
GTATTTGGCC TTGGCTCCCG GGCTTACCCT CATTTCTGCG CCTTTGGACA TGCCGTGGAC 
ACCCTCCTGG AGGAGCTGGG AGGAGAGAGG ATCCTGAAGA TGAGGGAGGG AGACGAGCTC 
TGTGGGCAGG AAGAGGCTTT CAGGACCTGG GCCAAGAAGG TCTTCAAGGC AGCCTGCGAT 
GTGTTCTGCG TGGGAGATGA CGTCAACATT GAGAAGGCAA ACAACTCCCT CATCAGCAAC 
GACCGCAGCT GGAAAAGAAA CAAGTTCCGC CTCACCTACG TGGCCGAAGC TCCGGAACTT 
ACACAAGGTC TATCCAATGT CCACAAAAAG CGGGTCTCGG CCGCTCGACT CCTCAGCCGC 
CAAAACCTTC AGAGCCCTAA ATCCAGCCGA TCAACGATCT TCGTGCGTCT CCACACCAAT 
GGGAATCAGG AGCTGCAGTA CCAGCCTGGG GACCACCTGG GCGTCTTCCC TGGCAACCGC 
GAGGACCTCG TGAACGCCCT GATGGAGCGG CTGGAAGATG CTCCCCCAGT CAACCAGCTG 
GTGAAGGTGG AACTGCTGGT GGAGAGGAAC ACGGCTTTGG GCGTCATCAG TAACTGGACA 
GATGAGCACC GCCTCCCGCC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACACCGC CCACGCCACT GCAGCTGCAG CAGTTTGCGT CCCTGGCCAC CAGCGAGAAA 
GAGAAGCAGC GACTGCTGGT CCTCAGCAAG GGCTTGCAGG AGTACGAGGA ATGGAAATGG 
GGCAAGAACC CCACCATCGT GGAGGTGCTG GAAGAGTTCC CGTCCATCCA GATGCCCACC 
ACCCTGCTCC TGACCCAGCT GTCCCTGCTG CAGCCTCGAT ACTATTCAAT CAGTTCTTCC 
CCAGACATGT ACCCTGACGA GGTGCACCTC ACCGTGGCCG TTGTCTCCTA CCACACCCGA 
GATGGAGAAG GACCAATTCA CCATGGCGTG TGCTCCTCCT GGCTCAACCG GATACCGGCT 
GATGAAGTGG TCCCCTGTTT CGTGAGAGGA GCACCCAGCT TCCACCTGCC CCGAAATCCC 
CAAGTGCCCT GTATCCTCAT TGGCCCAGGC ACTGGCATTG CCCCCTTCCG AAGCTTCTGG 
CAGCAGCGGC AATTTGATAT CCAGCACAAA GGAATGAGCC CCTGCCCCAT GGTCCTGGTG 
TTTGGGTGCC GGCAATCCAA GATAGACCAC ATCTACAGGG AGGAAACTCT ACAGGCCAAG 
AACAAGGGGG TCTTCAGAGA ACTCTACACG GCCTACTCGC GGGAGCCAGA CAAACCAAAG 
AAGTACGTTC AGGACATCCT GCAGGAGCAC TTGGCAGAAC CTGTGTACCG AGCCCTGAAG 
GAGCAAGGGG GCCACATTTA CGTCTGCGGG GACGTCACCA TGGCCGCCGA TGTCCTCAAA 
GCCATCCAGC GCATCATGAC CCAGCAAGGG AAACTCTCAG CGGAGGATGC CGGCGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CATGAGGATA TTTTCGGGGT CACCCTGCGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAATCCATTG CCTTCATTGA GGAGAGCAAA 
AAAGACACTG ATGAAGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011286105.1
CDS	724..5013
Translation	MENQVFSVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGQPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTRPLAPPTTAVDLSHQSSASREHSPAVDRATGPGNGPQHAQDAGQEAGSLPHTKSRAPGPPGQDSAKKSIQGSQNSDLLKEIEPVLSLLTSGSKGINGGGPSKAETKDAEVQVDRGMDGKSHKPLPLGVENDRVFSDLWGKGHVPVVLNNPYSEKEQPPASGKQSPTKNGSPSKCPRFLKVKNWETDVVLTDTLHLKSTLEMGCTEHICMGSIMLPSQQIRRPEDVRTKEQLFPLAKEFIDQYYSSIKRFGSKAHTERLEEVNKEIETTGTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHIKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSILGDPANVEFTEICVQQGWKPPRGRFDILPLLLQANGNDPELFQIPPELVLEVPIRHPRFEWFKDLGLKWYGLPAVSNMLLEIGGLEFTACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKNMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSVTPVFHQEMLNYRLTPSFEYQPEPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSHKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTYVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNREDLVNALMERLEDAPPVNQLVKVELLVERNTALGVISNWTDEHRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPTTLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAVVSYHTRDGEGPIHHGVCSSWLNRIPADEVVPCFVRGAPSFHLPRNPQVPCILIGPGTGIAPFRSFWQQRQFDIQHKGMSPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEHLAEPVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRLRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS

Target ORF information:

RefSeq Version	XM_011287803.3
Organism	Felis catus(domestic cat)
Definition	Felis catus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011287803.3

ORF Insert Sequence:

ATGGAGAATC AGGTGTTCAG CGTTCAGCAG ATCCAACCCA ACGTCATCTC CGTCCGCCTC 
TTCAAGCGCA AGGTGGGGGG CCTGGGGTTC CTGGTGAAGG AGCGGGTGAG CAAGCCGCCC 
GTGATCATCT CCGACCTGAT TCGGGGGGGT GCCGCAGAGC AGAGCGGCCT CATCCAGGCC 
GGTGACATCA TCCTTGCAGT CAACGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCC 
CTGGAGGTGC TCAGGGGCAT CGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCC 
GAGGGCTTCA CCACTCACCT GGAGACCACC TTCACTGGGG ACGGGACCCC CAAGACCATC 
CGCGTGACAC GGCCTCTGGC TCCCCCCACC ACCGCTGTCG ATCTGTCTCA CCAGTCATCG 
GCCAGCAGAG AGCACTCACC GGCAGTGGAC AGGGCCACGG GTCCTGGCAA TGGGCCGCAG 
CACGCCCAAG ACGCTGGGCA GGAAGCCGGC TCTCTCCCCC ATACCAAGAG CCGGGCTCCC 
GGGCCCCCGG GCCAGGACTC TGCCAAGAAG AGCATCCAGG GCAGCCAGAA CAGTGATTTG 
CTCAAAGAGA TAGAACCTGT ACTGAGCCTT CTCACCAGTG GGAGCAAAGG GATCAATGGC 
GGGGGACCTT CCAAGGCAGA GACGAAGGAT GCAGAAGTCC AGGTGGACAG AGGTATGGAT 
GGCAAGTCAC ACAAGCCACT GCCCCTGGGT GTGGAGAATG ACCGAGTCTT CAGTGACCTG 
TGGGGGAAGG GCCACGTGCC TGTCGTCCTC AACAACCCGT ATTCAGAGAA GGAGCAGCCC 
CCTGCCTCGG GAAAACAGTC TCCTACAAAG AACGGCAGCC CCTCCAAGTG CCCGCGCTTC 
CTCAAGGTCA AGAACTGGGA GACCGACGTG GTCCTCACTG ACACCCTTCA CCTTAAGAGC 
ACATTGGAAA TGGGCTGCAC CGAGCACATC TGTATGGGCT CCATCATGCT CCCCTCTCAG 
CAGATACGAA GGCCTGAAGA CGTCCGCACA AAAGAACAGC TCTTCCCTCT CGCCAAAGAG 
TTCATTGACC AGTACTACTC ATCAATTAAA AGGTTTGGCT CCAAAGCCCA CACGGAGAGG 
CTGGAAGAGG TGAACAAAGA GATCGAAACC ACCGGCACCT ACCAGCTCAA AGACACGGAG 
CTCATCTACG GGGCCAAGCA TGCCTGGCGG AACGCCTCCC GCTGCGTGGG CAGGATCCAG 
TGGTCCAAGC TGCAGGTGTT CGATGCCCGC GACTGCACCA CGGCTCACGG GATGTTCAAC 
TACATCTGCA ACCACATCAA GTATGCCACC AACAAGGGGA ACCTCAGATC CGCCATCACC 
ATATTCCCCC AGAGGACAGA CGGCAAGCAC GATTTCCGAG TCTGGAACTC TCAGCTCATC 
CGCTATGCCG GCTACAAGCA GCCTGATGGC TCCATCCTGG GGGACCCAGC CAATGTGGAA 
TTCACAGAGA TCTGTGTGCA GCAGGGCTGG AAACCCCCAA GAGGCCGCTT CGATATCCTG 
CCACTCCTGC TCCAGGCCAA CGGCAACGAT CCCGAGCTCT TCCAGATCCC CCCAGAGCTG 
GTGTTGGAAG TGCCCATCAG ACACCCCAGG TTTGAGTGGT TCAAGGATCT GGGCCTGAAG 
TGGTATGGCC TCCCCGCTGT GTCCAACATG CTGCTGGAGA TTGGCGGCCT GGAGTTCACC 
GCCTGTCCCT TCAGCGGCTG GTACATGGGC ACAGAGATTG GCGTCCGCGA CTATTGTGAC 
AACTCTCGAT ACAACATCCT GGAGGAAGTG GCCAAGAACA TGAACTTGGA CATGAGGAAG 
ACGTCCTCCC TGTGGAAGGA CCAAGCACTG GTGGAAATCA ACATTGCTGT TCTCTACAGC 
TTCCAGAGTG ACAAAGTGAC CATCGTTGAC CACCACTCTG CCACCGAGTC CTTCATCAAG 
CACATGGAGA ATGAATACCG CTGCCGGGGG GGCTGCCCCG CCGACTGGGT GTGGATTGTG 
CCCCCCATGT CTGGCAGTGT CACCCCTGTC TTCCACCAGG AGATGCTCAA CTACCGACTC 
ACCCCGTCCT TCGAATACCA GCCTGAGCCT TGGAACACCC ACGTCTGGAA AGGCACCAAT 
GGAACCCCCA CAAAGCGGCG AGCCATCGGC TTCAAGAAGC TGGCAGAAGC CGTCAAGTTC 
TCAGCCAAGC TTATGGGGCA GGCAATGGCC AAGAGGGTCA AGGCGACCAT CCTTTATGCC 
ACAGAGACGG GCAAGTCGCA GGCCTATGCT AAAACCTTGT GTGAGATCTT CAAACATGCC 
TTTGATGCCA AGGTGATGTC CATGGAAGAA TATGACATTG TACACCTGGA ACATGAAACT 
CTGGTCCTAG TGGTTACCAG CACTTTTGGC AATGGCGACC CCCCAGAGAA TGGGGAGAAA 
TTTGGCTGTG CTTTGATGGA AATGAGGCAC CCCAACTCCG TGCAGGAGGA AAGGAAGAGC 
TACAAGGTCC GATTCAATAG TGTTTCCTCG TATTCTGACT CCCATAAATC ATCAGGCGAC 
GGGCCAGACC TCAGAGACAA CTTTGAGAGT GCTGGACCCC TGGCCAACGT GAGGTTCTCC 
GTATTTGGCC TTGGCTCCCG GGCTTACCCT CATTTCTGCG CCTTTGGACA TGCCGTGGAC 
ACCCTCCTGG AGGAGCTGGG AGGAGAGAGG ATCCTGAAGA TGAGGGAGGG AGACGAGCTC 
TGTGGGCAGG AAGAGGCTTT CAGGACCTGG GCCAAGAAGG TCTTCAAGGC AGCCTGCGAT 
GTGTTCTGCG TGGGAGATGA CGTCAACATT GAGAAGGCAA ACAACTCCCT CATCAGCAAC 
GACCGCAGCT GGAAAAGAAA CAAGTTCCGC CTCACCTACG TGGCCGAAGC TCCGGAACTT 
ACACAAGGTC TATCCAATGT CCACAAAAAG CGGGTCTCGG CCGCTCGACT CCTCAGCCGC 
CAAAACCTTC AGAGCCCTAA ATCCAGCCGA TCAACGATCT TCGTGCGTCT CCACACCAAT 
GGGAATCAGG AGCTGCAGTA CCAGCCTGGG GACCACCTGG GCGTCTTCCC TGGCAACCGC 
GAGGACCTCG TGAACGCCCT GATGGAGCGG CTGGAAGATG CTCCCCCAGT CAACCAGCTG 
GTGAAGGTGG AACTGCTGGT GGAGAGGAAC ACGGCTTTGG GCGTCATCAG TAACTGGACA 
GATGAGCACC GCCTCCCGCC CTGCACCATC TTCCAGGCCT TCAAGTACTA CCTGGACATC 
ACCACACCGC CCACGCCACT GCAGCTGCAG CAGTTTGCGT CCCTGGCCAC CAGCGAGAAA 
GAGAAGCAGC GACTGCTGGT CCTCAGCAAG GGCTTGCAGG AGTACGAGGA ATGGAAATGG 
GGCAAGAACC CCACCATCGT GGAGGTGCTG GAAGAGTTCC CGTCCATCCA GATGCCCACC 
ACCCTGCTCC TGACCCAGCT GTCCCTGCTG CAGCCTCGAT ACTATTCAAT CAGTTCTTCC 
CCAGACATGT ACCCTGACGA GGTGCACCTC ACCGTGGCCG TTGTCTCCTA CCACACCCGA 
GATGGAGAAG GACCAATTCA CCATGGCGTG TGCTCCTCCT GGCTCAACCG GATACCGGCT 
GATGAAGTGG TCCCCTGTTT CGTGAGAGGA GCACCCAGCT TCCACCTGCC CCGAAATCCC 
CAAGTGCCCT GTATCCTCAT TGGCCCAGGC ACTGGCATTG CCCCCTTCCG AAGCTTCTGG 
CAGCAGCGGC AATTTGATAT CCAGCACAAA GGAATGAGCC CCTGCCCCAT GGTCCTGGTG 
TTTGGGTGCC GGCAATCCAA GATAGACCAC ATCTACAGGG AGGAAACTCT ACAGGCCAAG 
AACAAGGGGG TCTTCAGAGA ACTCTACACG GCCTACTCGC GGGAGCCAGA CAAACCAAAG 
AAGTACGTTC AGGACATCCT GCAGGAGCAC TTGGCAGAAC CTGTGTACCG AGCCCTGAAG 
GAGCAAGGGG GCCACATTTA CGTCTGCGGG GACGTCACCA TGGCCGCCGA TGTCCTCAAA 
GCCATCCAGC GCATCATGAC CCAGCAAGGG AAACTCTCAG CGGAGGATGC CGGCGTGTTC 
ATCAGCAGGC TGAGGGATGA CAACCGGTAC CATGAGGATA TTTTCGGGGT CACCCTGCGA 
ACATATGAAG TGACCAACCG CCTTAGATCT GAATCCATTG CCTTCATTGA GGAGAGCAAA 
AAAGACACTG ATGAAGTTTT CAGCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NOS1 cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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