DYSF gene cDNA ORF clone, Felis catus(Domestic cat)

mRNA	Protein	Name
XM_003984107.4	XP_003984156.1	dysferlin isoform X12
XM_003984109.4	XP_003984158.1	dysferlin isoform X15
XM_003984110.4	XP_003984159.1	dysferlin isoform X9
XM_003984110.5	XP_003984159.2	LOW QUALITY PROTEIN: dysferlin
XM_003984112.4	XP_003984161.1	dysferlin isoform X11
XM_019827392.1	XP_019682951.1	dysferlin isoform X1
XM_019827398.1	XP_019682957.1	dysferlin isoform X16
XM_003984111.4	XP_003984160.1	dysferlin isoform X13
XM_019827394.1	XP_019682953.1	dysferlin isoform X4
XM_019827396.1	XP_019682955.1	dysferlin isoform X7
XM_019827393.1	XP_019682952.1	dysferlin isoform X3
XM_003984108.4	XP_003984157.1	dysferlin isoform X14
XM_003984113.4	XP_003984162.1	dysferlin isoform X8
XM_006930175.3	XP_006930237.1	dysferlin isoform X2
XM_006930178.3	XP_006930240.1	dysferlin isoform X6
XM_019827395.1	XP_019682954.1	dysferlin isoform X5
XM_019827397.1	XP_019682956.1	dysferlin isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb40436	XM_019827398.1 Latest version!	Felis catus dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb04556	XM_006930175.3 Latest version!	Felis catus dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04559	XM_006930178.3 Latest version!	Felis catus dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04561	XM_003984113.4 Latest version!	Felis catus dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04563	XM_003984110.4 Latest version!	Felis catus dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04564	XM_003984112.4 Latest version!	Felis catus dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04565	XM_003984107.4 Latest version!	Felis catus dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04566	XM_003984111.4 Latest version!	Felis catus dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04567	XM_003984108.4 Latest version!	Felis catus dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb04568	XM_003984109.4 Latest version!	Felis catus dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb40430	XM_019827392.1 Latest version!	Felis catus dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb40431	XM_019827393.1 Latest version!	Felis catus dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb40432	XM_019827394.1 Latest version!	Felis catus dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb40433	XM_019827395.1 Latest version!	Felis catus dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb40434	XM_019827396.1 Latest version!	Felis catus dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb40435	XM_019827397.1 Latest version!	Felis catus dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb73655	XM_003984110.5 Latest version!	Felis catus dysferlin (DYSF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

DYSF ( XM_019827398.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682957.1 Felis catus dysferlin (DYSF), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OFb40436
Clone ID Related Accession (Same CDS sequence)	XM_019827398.1
Accession Version	XM_019827398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCC TCATAAGCCC 
CTCCTGGAGG AGGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG 
GAAAAGTGTG GCCAGTACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA 
CTGGAGAATG TGGAAGCCTT CGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA GACCGAAGAC CCATCTGTTA TTGGGGAGTT CAAGGGCCTC 
TTCAAAATCT ATCCCCTCCC AGAGGACCCT GCCATTCCCA TGCCCCCAAG ACAGTTCCAC 
CAACTGGCTG CCCAGGGGCC TCAGGAGTGC CTGGTCCGCA TCTACATCAT CCGAGCATTC 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCTTGTA CTCTGGAGCC TGTGTTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATCGA CCTGGAGAAT 
AGGCTGCTGT CCAAGTTTGG AGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AAGCTCCTGT GTACCACACA GACCGAGTGG TGTTTGAGGA TAAAGAATAC 
ACCATTGAGG AAATAGAGGC TGGCAAGGTC CTGAACCCAC ACCTGGGCCC AGTGGAAGAG 
CGTCTGGCCT TGTATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC 
CTATTTCCAA AGGCCCTGGG GAGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGCTG TATTATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC 
AGCATCACAG GGGAGAAGAT GAGCGATATT TACGTGAAAG GTTGGATGGT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA TGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTTAAC 
TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCTGAGC AAGTCTGCAC TGTCTCTAAG 
AAGGATCGTC ACAATGTTTC CTGTCCTTGC CACTCACTCA GCAGAAGCTC CAGCAAGAGG 
ACAGAGGACA AGCGGGAGCA GGAAAGCCCC AGTGACAGTC CTTTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682957.1
CDS	417..6407
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHKPLLEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDRHNVSCPCHSLSRSSSKRTEDKREQESPSDSPLG

Target ORF information:

RefSeq Version	XM_019827398.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827398.1

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCC TCATAAGCCC 
CTCCTGGAGG AGGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG 
GAAAAGTGTG GCCAGTACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA 
CTGGAGAATG TGGAAGCCTT CGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA GACCGAAGAC CCATCTGTTA TTGGGGAGTT CAAGGGCCTC 
TTCAAAATCT ATCCCCTCCC AGAGGACCCT GCCATTCCCA TGCCCCCAAG ACAGTTCCAC 
CAACTGGCTG CCCAGGGGCC TCAGGAGTGC CTGGTCCGCA TCTACATCAT CCGAGCATTC 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCTTGTA CTCTGGAGCC TGTGTTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATCGA CCTGGAGAAT 
AGGCTGCTGT CCAAGTTTGG AGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AAGCTCCTGT GTACCACACA GACCGAGTGG TGTTTGAGGA TAAAGAATAC 
ACCATTGAGG AAATAGAGGC TGGCAAGGTC CTGAACCCAC ACCTGGGCCC AGTGGAAGAG 
CGTCTGGCCT TGTATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC 
CTATTTCCAA AGGCCCTGGG GAGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGCTG TATTATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC 
AGCATCACAG GGGAGAAGAT GAGCGATATT TACGTGAAAG GTTGGATGGT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA TGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTTAAC 
TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCTGAGC AAGTCTGCAC TGTCTCTAAG 
AAGGATCGTC ACAATGTTTC CTGTCCTTGC CACTCACTCA GCAGAAGCTC CAGCAAGAGG 
ACAGAGGACA AGCGGGAGCA GGAAAGCCCC AGTGACAGTC CTTTGGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_006930175.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_006930237.1 Felis catus dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OFb04556
Clone ID Related Accession (Same CDS sequence)	XM_006930175.3
Accession Version	XM_006930175.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006930175.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCC TCATGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA AAAGTGTGGC 
CAGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG 
GAAGCCTTCG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CCGAAGACCC ATCTGTTATT GGGGAGTTCA AGGGCCTCTT CAAAATCTAT 
CCCCTCCCAG AGGACCCTGC CATTCCCATG CCCCCAAGAC AGTTCCACCA ACTGGCTGCC 
CAGGGGCCTC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTCGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT TCCTTGTACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAAG ATGAAAAGAT TGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGAG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAA 
GCTCCTGTGT ACCACACAGA CCGAGTGGTG TTTGAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAAGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAGCATGTGG AGTCCCGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA ACAGGGGAAG CTGCAGATGT GGATCGACCT ATTTCCAAAG 
GCCCTGGGGA GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGCTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACAGGG 
GAGAAGATGA GCGATATTTA CGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC AGCTGAGCAA GTCTGCACTG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TCATATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTA GGCTCCTTGC AGCTGGACCT CAACCACATG 
CCCAAACCTG CCAAGACAGC TGAGAAATGC TCCTTGGACC AGCTGGATGG CACCTTCCAC 
CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGAGG GCGAGAAGAA GATCCTGGCG GGCAAACTGG AAATGACCTT GGAGATCGTA 
GCAGAGAATG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAACC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAT ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC 
AAGACTATGA AGTTCATCCT ATGGCGGCGC TTCCGGTGTG CCATTATTCT CTTCATCATT 
CTCTTCATCC TACTGCTGTT CCTGGGCATC TTCGTCTATG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006930237.1
CDS	409..6855
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_006930175.3
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006930175.3

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCC TCATGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA AAAGTGTGGC 
CAGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG 
GAAGCCTTCG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CCGAAGACCC ATCTGTTATT GGGGAGTTCA AGGGCCTCTT CAAAATCTAT 
CCCCTCCCAG AGGACCCTGC CATTCCCATG CCCCCAAGAC AGTTCCACCA ACTGGCTGCC 
CAGGGGCCTC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTCGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT TCCTTGTACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAAG ATGAAAAGAT TGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGAG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAA 
GCTCCTGTGT ACCACACAGA CCGAGTGGTG TTTGAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAAGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAGCATGTGG AGTCCCGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA ACAGGGGAAG CTGCAGATGT GGATCGACCT ATTTCCAAAG 
GCCCTGGGGA GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGCTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACAGGG 
GAGAAGATGA GCGATATTTA CGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC AGCTGAGCAA GTCTGCACTG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TCATATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTA GGCTCCTTGC AGCTGGACCT CAACCACATG 
CCCAAACCTG CCAAGACAGC TGAGAAATGC TCCTTGGACC AGCTGGATGG CACCTTCCAC 
CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGAGG GCGAGAAGAA GATCCTGGCG GGCAAACTGG AAATGACCTT GGAGATCGTA 
GCAGAGAATG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAACC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAT ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC 
AAGACTATGA AGTTCATCCT ATGGCGGCGC TTCCGGTGTG CCATTATTCT CTTCATCATT 
CTCTTCATCC TACTGCTGTT CCTGGGCATC TTCGTCTATG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_006930178.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_006930240.1 Felis catus dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OFb04559
Clone ID Related Accession (Same CDS sequence)	XM_006930178.3
Accession Version	XM_006930178.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_006930178.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GGAGGAGGAG 
TTCATCGACT GGTGGAGCAA ATTCTTTGCC TCCACAGGGG AGAGGGAAAA GTGTGGCCAG 
TACTTGGAGA AGGGCTTTGA CACTCTAAAG GTCTATGACA CACAACTGGA GAATGTGGAA 
GCCTTCGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGAGACCG AAGACCCATC TGTTATTGGG GAGTTCAAGG GCCTCTTCAA AATCTATCCC 
CTCCCAGAGG ACCCTGCCAT TCCCATGCCC CCAAGACAGT TCCACCAACT GGCTGCCCAG 
GGGCCTCAGG AGTGCCTGGT CCGCATCTAC ATCATCCGAG CATTCGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATTCC TTGTACTCTG GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAAGATG AAAAGATTGG GGAGACAGTC ATCGACCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGAGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCCAA CCAGTGGAGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAAGCT 
CCTGTGTACC ACACAGACCG AGTGGTGTTT GAGGATAAAG AATACACCAT TGAGGAAATA 
GAGGCTGGCA AGGTCCTGAA CCCACACCTG GGCCCAGTGG AAGAGCGTCT GGCCTTGTAT 
GTCCTCCAGC AGCAGGGCCT GGTCCCTGAG CATGTGGAGT CCCGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAACA GGGGAAGCTG CAGATGTGGA TCGACCTATT TCCAAAGGCC 
CTGGGGAGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGCTGTATTA TCTGGAACAC AAAGGACGTG ATCCTGGACG ACCTCAGCAT CACAGGGGAG 
AAGATGAGCG ATATTTACGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCATTTTC 
CCCTTTGACT ACCTGCCAGC TGAGCAAGTC TGCACTGTCT CTAAGAAGGA TGCTTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTCA TATTTCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGACGA CTTTCTAGGC TCCTTGCAGC TGGACCTCAA CCACATGCCC 
AAACCTGCCA AGACAGCTGA GAAATGCTCC TTGGACCAGC TGGATGGCAC CTTCCACCCA 
GAGTGGTTTG TGTCCCTTTT TGAACAGAAA ACTGTGAAGG GCTGGTGGCC CTGTGTGGCA 
GAAGAGGGCG AGAAGAAGAT CCTGGCGGGC AAACTGGAAA TGACCTTGGA GATCGTAGCA 
GAGAATGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAACCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGATACC TCCTTCCTCT GGTTCACCTC TCCGTACAAG 
ACTATGAAGT TCATCCTATG GCGGCGCTTC CGGTGTGCCA TTATTCTCTT CATCATTCTC 
TTCATCCTAC TGCTGTTCCT GGGCATCTTC GTCTATGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006930240.1
CDS	409..6792
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_006930178.3
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006930178.3

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GGAGGAGGAG 
TTCATCGACT GGTGGAGCAA ATTCTTTGCC TCCACAGGGG AGAGGGAAAA GTGTGGCCAG 
TACTTGGAGA AGGGCTTTGA CACTCTAAAG GTCTATGACA CACAACTGGA GAATGTGGAA 
GCCTTCGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGAGACCG AAGACCCATC TGTTATTGGG GAGTTCAAGG GCCTCTTCAA AATCTATCCC 
CTCCCAGAGG ACCCTGCCAT TCCCATGCCC CCAAGACAGT TCCACCAACT GGCTGCCCAG 
GGGCCTCAGG AGTGCCTGGT CCGCATCTAC ATCATCCGAG CATTCGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATTCC TTGTACTCTG GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAAGATG AAAAGATTGG GGAGACAGTC ATCGACCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGAGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCCAA CCAGTGGAGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAAGCT 
CCTGTGTACC ACACAGACCG AGTGGTGTTT GAGGATAAAG AATACACCAT TGAGGAAATA 
GAGGCTGGCA AGGTCCTGAA CCCACACCTG GGCCCAGTGG AAGAGCGTCT GGCCTTGTAT 
GTCCTCCAGC AGCAGGGCCT GGTCCCTGAG CATGTGGAGT CCCGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAACA GGGGAAGCTG CAGATGTGGA TCGACCTATT TCCAAAGGCC 
CTGGGGAGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGCTGTATTA TCTGGAACAC AAAGGACGTG ATCCTGGACG ACCTCAGCAT CACAGGGGAG 
AAGATGAGCG ATATTTACGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCATTTTC 
CCCTTTGACT ACCTGCCAGC TGAGCAAGTC TGCACTGTCT CTAAGAAGGA TGCTTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTCA TATTTCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGACGA CTTTCTAGGC TCCTTGCAGC TGGACCTCAA CCACATGCCC 
AAACCTGCCA AGACAGCTGA GAAATGCTCC TTGGACCAGC TGGATGGCAC CTTCCACCCA 
GAGTGGTTTG TGTCCCTTTT TGAACAGAAA ACTGTGAAGG GCTGGTGGCC CTGTGTGGCA 
GAAGAGGGCG AGAAGAAGAT CCTGGCGGGC AAACTGGAAA TGACCTTGGA GATCGTAGCA 
GAGAATGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAACCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGATACC TCCTTCCTCT GGTTCACCTC TCCGTACAAG 
ACTATGAAGT TCATCCTATG GCGGCGCTTC CGGTGTGCCA TTATTCTCTT CATCATTCTC 
TTCATCCTAC TGCTGTTCCT GGGCATCTTC GTCTATGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984113.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984162.1 Felis catus dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OFb04561
Clone ID Related Accession (Same CDS sequence)	XM_003984113.4
Accession Version	XM_003984113.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984113.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT TGGGCCCTGC 
TATGTCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCAGAA 
CTGAACATGG GCAAGGGGGA AGGTGTGGCC TACCGAGGTC GGGTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TATTCCCTGT TTGCTGCCTT TTACTCGGCC 
TCCATGCTCC AGGATGTTGA TGATGCCATC CAGTTTGAGG TCAGCATCGG GAACTACGGT 
AACAAGTTCG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCTTACT GGGAGGACAT CAGCCACCGG GTCGAGGCTC AGAACCAGCT GCTCAGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCAGC 
CCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC CGACTGCAGC 
CAGCCCCTAG GCGACGTCCA AGAGATACCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
AAGCTGCGTA CCCGTCACCT AGGCCAGATC ACTGAGGCGG CCCTGGCACT GAAACTTGGC 
CGCAGCGAGC TCCCTGCTGC CCTGGAGCAG GCGGAAGACT GGCTGCTGCG TGTCCGGGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGAGTGG CGTACCAGCG GGTGCCCGCC CACGAGATCC TCTTCTCCCG GCGGGGTCCC 
AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATAT TTCTGAAATA TCCAATGGAG 
GGCGTTCCTG GAGCCCGGAT GCCAGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCCGTCTT TGCTGAAACG 
TATGAGAACC AGACCAAACT GGCCCTGGTT GGGAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGAAAGATT AAGCTACCCA AAGATAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATTGACGCGG GACACCTGAG TTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCCCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCCCCAT CCCCCCGGAT 
CGGAAGCCAA AGCACTGGGT TCCTGCTGAG AAGATGTACT ACACGCACCG TCGACGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACGGATGC CTTCCGCCGT CGCCGCTGGC GTCGCCGCAT GGAGCCCCTG 
GAGAAGACAG GGCCTGCGGC CGTGTTTGCC CTCGAGGGGG CTCTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATTA TGGGAACCGC TACCACCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGCG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTATGAGAT TGAGATCTTT GGTGAGCCAG CCAGCATCGC CGAGCAACCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTACG GTGCCGACGA GTTCATGGGA 
CGCTGCATCT GTCAGCCAAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGTTGATT 
AGGGGCAGCC AGCCAGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCAGGAAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC GTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGT GCTCCAGCGT ACCGCCATAG AGATCCTGGC ATGGGGCCTG 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCTC CCAGCCTTGT GGTGGAGTGT 
GGAGGCCAGA CCGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTTGAC 
ATCTGTACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
GTCCGCTCCC TGGAGAGCTT CATGTGTGAC CAGTACTCTG AAGAGAGTCC GTCCCCGCAG 
GGCGGCCCAG ATGACGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GATAAGGAGC CCCTCATCCC CATCCAGCTT GCAGATGGTC TGTCGGGCTC TGCCCCCACT 
AACATGGCAT CCTCTCCATC CAGTCCTCAT GAGGAGGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGTGGCCAGT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AATGTGGAAG CCTTCGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACCGA AGACCCATCT 
GTTATTGGGG AGTTCAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAGGA CCCTGCCATT 
CCCATGCCCC CAAGACAGTT CCACCAACTG GCTGCCCAGG GGCCTCAGGA GTGCCTGGTC 
CGCATCTACA TCATCCGAGC ATTCGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCT 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TTGGAGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAAGCTC CTGTGTACCA CACAGACCGA 
GTGGTGTTTG AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAA GGTCCTGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGTATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCAAAGGCCC TGGGGAGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GCTGTATTAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGCGA TATTTACGTG 
AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCT 
GAGCAAGTCT GCACTGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTCAT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTAGGCT CCTTGCAGCT GGACCTCAAC CACATGCCCA AACCTGCCAA GACAGCTGAG 
AAATGCTCCT TGGACCAGCT GGATGGCACC TTCCACCCAG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CTGTGAAGGG CTGGTGGCCC TGTGTGGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCGGGCA AACTGGAAAT GACCTTGGAG ATCGTAGCAG AGAATGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGATACCT CCTTCCTCTG GTTCACCTCT CCGTACAAGA CTATGAAGTT CATCCTATGG 
CGGCGCTTCC GGTGTGCCAT TATTCTCTTC ATCATTCTCT TCATCCTACT GCTGTTCCTG 
GGCATCTTCG TCTATGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984162.1
CDS	409..6771
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984113.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984113.4

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT TGGGCCCTGC 
TATGTCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCAGAA 
CTGAACATGG GCAAGGGGGA AGGTGTGGCC TACCGAGGTC GGGTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TATTCCCTGT TTGCTGCCTT TTACTCGGCC 
TCCATGCTCC AGGATGTTGA TGATGCCATC CAGTTTGAGG TCAGCATCGG GAACTACGGT 
AACAAGTTCG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCTTACT GGGAGGACAT CAGCCACCGG GTCGAGGCTC AGAACCAGCT GCTCAGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCAGC 
CCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC CGACTGCAGC 
CAGCCCCTAG GCGACGTCCA AGAGATACCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
AAGCTGCGTA CCCGTCACCT AGGCCAGATC ACTGAGGCGG CCCTGGCACT GAAACTTGGC 
CGCAGCGAGC TCCCTGCTGC CCTGGAGCAG GCGGAAGACT GGCTGCTGCG TGTCCGGGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGAGTGG CGTACCAGCG GGTGCCCGCC CACGAGATCC TCTTCTCCCG GCGGGGTCCC 
AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATAT TTCTGAAATA TCCAATGGAG 
GGCGTTCCTG GAGCCCGGAT GCCAGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCCGTCTT TGCTGAAACG 
TATGAGAACC AGACCAAACT GGCCCTGGTT GGGAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGAAAGATT AAGCTACCCA AAGATAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATTGACGCGG GACACCTGAG TTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCCCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCCCCAT CCCCCCGGAT 
CGGAAGCCAA AGCACTGGGT TCCTGCTGAG AAGATGTACT ACACGCACCG TCGACGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACGGATGC CTTCCGCCGT CGCCGCTGGC GTCGCCGCAT GGAGCCCCTG 
GAGAAGACAG GGCCTGCGGC CGTGTTTGCC CTCGAGGGGG CTCTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATTA TGGGAACCGC TACCACCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGCG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTATGAGAT TGAGATCTTT GGTGAGCCAG CCAGCATCGC CGAGCAACCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTACG GTGCCGACGA GTTCATGGGA 
CGCTGCATCT GTCAGCCAAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGTTGATT 
AGGGGCAGCC AGCCAGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCAGGAAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC GTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGT GCTCCAGCGT ACCGCCATAG AGATCCTGGC ATGGGGCCTG 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCTC CCAGCCTTGT GGTGGAGTGT 
GGAGGCCAGA CCGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTTGAC 
ATCTGTACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
GTCCGCTCCC TGGAGAGCTT CATGTGTGAC CAGTACTCTG AAGAGAGTCC GTCCCCGCAG 
GGCGGCCCAG ATGACGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GATAAGGAGC CCCTCATCCC CATCCAGCTT GCAGATGGTC TGTCGGGCTC TGCCCCCACT 
AACATGGCAT CCTCTCCATC CAGTCCTCAT GAGGAGGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGTGGCCAGT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AATGTGGAAG CCTTCGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACCGA AGACCCATCT 
GTTATTGGGG AGTTCAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAGGA CCCTGCCATT 
CCCATGCCCC CAAGACAGTT CCACCAACTG GCTGCCCAGG GGCCTCAGGA GTGCCTGGTC 
CGCATCTACA TCATCCGAGC ATTCGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCT 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACTCT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TCGACCTGGA GAATAGGCTG CTGTCCAAGT TTGGAGCTCG CTGTGGACTC 
CCACAGACCT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAAGCTC CTGTGTACCA CACAGACCGA 
GTGGTGTTTG AGGATAAAGA ATACACCATT GAGGAAATAG AGGCTGGCAA GGTCCTGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGTATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCAAAGGCCC TGGGGAGGCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GCTGTATTAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGCGA TATTTACGTG 
AAAGGTTGGA TGGTTGGCTT TGAAGAACAC AAGCAAAAGA CAGATGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCT 
GAGCAAGTCT GCACTGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTCAT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTAGGCT CCTTGCAGCT GGACCTCAAC CACATGCCCA AACCTGCCAA GACAGCTGAG 
AAATGCTCCT TGGACCAGCT GGATGGCACC TTCCACCCAG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CTGTGAAGGG CTGGTGGCCC TGTGTGGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCGGGCA AACTGGAAAT GACCTTGGAG ATCGTAGCAG AGAATGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCTGATACCT CCTTCCTCTG GTTCACCTCT CCGTACAAGA CTATGAAGTT CATCCTATGG 
CGGCGCTTCC GGTGTGCCAT TATTCTCTTC ATCATTCTCT TCATCCTACT GCTGTTCCTG 
GGCATCTTCG TCTATGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984110.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984159.1 Felis catus dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OFb04563
Clone ID Related Accession (Same CDS sequence)	XM_003984110.4
Accession Version	XM_003984110.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984110.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGG AGGAGGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAAAAGT GTGGCCAGTA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ATGTGGAAGC CTTCGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACCGAA GACCCATCTG TTATTGGGGA GTTCAAGGGC 
CTCTTCAAAA TCTATCCCCT CCCAGAGGAC CCTGCCATTC CCATGCCCCC AAGACAGTTC 
CACCAACTGG CTGCCCAGGG GCCTCAGGAG TGCCTGGTCC GCATCTACAT CATCCGAGCA 
TTCGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCTT GTACTCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT CGACCTGGAG 
AATAGGCTGC TGTCCAAGTT TGGAGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAAGCTCC TGTGTACCAC ACAGACCGAG TGGTGTTTGA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAAG GTCCTGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGTATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC 
GACCTATTTC CAAAGGCCCT GGGGAGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG CTGTATTATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CAGGGGAGAA GATGAGCGAT ATTTACGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCTG AGCAAGTCTG CACTGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACAAGTCATA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTAGGCTC CTTGCAGCTG 
GACCTCAACC ACATGCCCAA ACCTGCCAAG ACAGCTGAGA AATGCTCCTT GGACCAGCTG 
GATGGCACCT TCCACCCAGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC TGTGAAGGGC 
TGGTGGCCCT GTGTGGCAGA AGAGGGCGAG AAGAAGATCC TGGCGGGCAA ACTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAATGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAACCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CTGATACCTC CTTCCTCTGG 
TTCACCTCTC CGTACAAGAC TATGAAGTTC ATCCTATGGC GGCGCTTCCG GTGTGCCATT 
ATTCTCTTCA TCATTCTCTT CATCCTACTG CTGTTCCTGG GCATCTTCGT CTATGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984159.1
CDS	409..6750
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984110.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984110.4

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGG AGGAGGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAAAAGT GTGGCCAGTA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ATGTGGAAGC CTTCGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACCGAA GACCCATCTG TTATTGGGGA GTTCAAGGGC 
CTCTTCAAAA TCTATCCCCT CCCAGAGGAC CCTGCCATTC CCATGCCCCC AAGACAGTTC 
CACCAACTGG CTGCCCAGGG GCCTCAGGAG TGCCTGGTCC GCATCTACAT CATCCGAGCA 
TTCGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCTT GTACTCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT CGACCTGGAG 
AATAGGCTGC TGTCCAAGTT TGGAGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAAGCTCC TGTGTACCAC ACAGACCGAG TGGTGTTTGA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAAG GTCCTGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGTATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC 
GACCTATTTC CAAAGGCCCT GGGGAGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG CTGTATTATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CAGGGGAGAA GATGAGCGAT ATTTACGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCTG AGCAAGTCTG CACTGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACAAGTCATA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTAGGCTC CTTGCAGCTG 
GACCTCAACC ACATGCCCAA ACCTGCCAAG ACAGCTGAGA AATGCTCCTT GGACCAGCTG 
GATGGCACCT TCCACCCAGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC TGTGAAGGGC 
TGGTGGCCCT GTGTGGCAGA AGAGGGCGAG AAGAAGATCC TGGCGGGCAA ACTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAATGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAACCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CTGATACCTC CTTCCTCTGG 
TTCACCTCTC CGTACAAGAC TATGAAGTTC ATCCTATGGC GGCGCTTCCG GTGTGCCATT 
ATTCTCTTCA TCATTCTCTT CATCCTACTG CTGTTCCTGG GCATCTTCGT CTATGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984112.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984161.1 Felis catus dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OFb04564
Clone ID Related Accession (Same CDS sequence)	XM_003984112.4
Accession Version	XM_003984112.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984112.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCG TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGTG CTCCAGCGTA CCGCCATAGA GATCCTGGCA 
TGGGGCCTGC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCTCC CAGCCTTGTG 
GTGGAGTGTG GAGGCCAGAC CGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTTGACA TCTGTACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCG TCCGCTCCCT GGAGAGCTTC ATGTGTGACC AGTACTCTGA AGAGAGTCCG 
TCCCCGCAGG GCGGCCCAGA TGACGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ATAAGGAGCC CCTCATCCCC ATCCAGCTTG CAGATGGTCT GTCGGGCTCT 
GCCCCCACTA ACATGGCATC CTCTCCATCC AGTCCTCATG AGGAGGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GTGGCCAGTA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ATGTGGAAGC CTTCGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACCGAA 
GACCCATCTG TTATTGGGGA GTTCAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAGGAC 
CCTGCCATTC CCATGCCCCC AAGACAGTTC CACCAACTGG CTGCCCAGGG GCCTCAGGAG 
TGCCTGGTCC GCATCTACAT CATCCGAGCA TTCGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCTT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACTCTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT TGGAGCTCGC 
TGTGGACTCC CACAGACCTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCGCAGAG TCAAAGCTCC TGTGTACCAC 
ACAGACCGAG TGGTGTTTGA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAAG 
GTCCTGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGTATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CAAAGGCCCT GGGGAGGCCT 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGAAGGT TTTTCCTGCG CTGTATTATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGCGAT 
ATTTACGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTGCAT 
TATCGCTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCTG AGCAAGTCTG CACTGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACAAGTCATA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTAGGCTC CTTGCAGCTG GACCTCAACC ACATGCCCAA ACCTGCCAAG 
ACAGCTGAGA AATGCTCCTT GGACCAGCTG GATGGCACCT TCCACCCAGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC TGTGAAGGGC TGGTGGCCCT GTGTGGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCGGGCAA ACTGGAAATG ACCTTGGAGA TCGTAGCAGA GAATGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCTAA GCTTGAGGAC 
CCAAGGCGCC CTGATACCTC CTTCCTCTGG TTCACCTCTC CGTACAAGAC TATGAAGTTC 
ATCCTATGGC GGCGCTTCCG GTGTGCCATT ATTCTCTTCA TCATTCTCTT CATCCTACTG 
CTGTTCCTGG GCATCTTCGT CTATGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984161.1
CDS	409..6720
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLADTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984112.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984112.4

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCG TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGTG CTCCAGCGTA CCGCCATAGA GATCCTGGCA 
TGGGGCCTGC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCTCC CAGCCTTGTG 
GTGGAGTGTG GAGGCCAGAC CGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTTGACA TCTGTACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCG TCCGCTCCCT GGAGAGCTTC ATGTGTGACC AGTACTCTGA AGAGAGTCCG 
TCCCCGCAGG GCGGCCCAGA TGACGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ATAAGGAGCC CCTCATCCCC ATCCAGCTTG CAGATGGTCT GTCGGGCTCT 
GCCCCCACTA ACATGGCATC CTCTCCATCC AGTCCTCATG AGGAGGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GTGGCCAGTA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ATGTGGAAGC CTTCGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACCGAA 
GACCCATCTG TTATTGGGGA GTTCAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAGGAC 
CCTGCCATTC CCATGCCCCC AAGACAGTTC CACCAACTGG CTGCCCAGGG GCCTCAGGAG 
TGCCTGGTCC GCATCTACAT CATCCGAGCA TTCGGCCTGC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCTT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACTCTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT CGACCTGGAG AATAGGCTGC TGTCCAAGTT TGGAGCTCGC 
TGTGGACTCC CACAGACCTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TCCTCCACCT CTTCTGCCAG CAGCGCAGAG TCAAAGCTCC TGTGTACCAC 
ACAGACCGAG TGGTGTTTGA GGATAAAGAA TACACCATTG AGGAAATAGA GGCTGGCAAG 
GTCCTGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGTATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CAAAGGCCCT GGGGAGGCCT 
GGACCTCCCT TCAACATCAC CCCACGGAGA GCCAGAAGGT TTTTCCTGCG CTGTATTATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGCGAT 
ATTTACGTGA AAGGTTGGAT GGTTGGCTTT GAAGAACACA AGCAAAAGAC AGATGTGCAT 
TATCGCTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCTG AGCAAGTCTG CACTGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACAAGTCATA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTAGGCTC CTTGCAGCTG GACCTCAACC ACATGCCCAA ACCTGCCAAG 
ACAGCTGAGA AATGCTCCTT GGACCAGCTG GATGGCACCT TCCACCCAGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC TGTGAAGGGC TGGTGGCCCT GTGTGGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCGGGCAA ACTGGAAATG ACCTTGGAGA TCGTAGCAGA GAATGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCTAA GCTTGAGGAC 
CCAAGGCGCC CTGATACCTC CTTCCTCTGG TTCACCTCTC CGTACAAGAC TATGAAGTTC 
ATCCTATGGC GGCGCTTCCG GTGTGCCATT ATTCTCTTCA TCATTCTCTT CATCCTACTG 
CTGTTCCTGG GCATCTTCGT CTATGCCTTC CCGAACTATG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984107.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984156.1 Felis catus dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OFb04565
Clone ID Related Accession (Same CDS sequence)	XM_003984107.4
Accession Version	XM_003984107.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984107.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT TGGGCCCTGC 
TATGTCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCAGAA 
CTGAACATGG GCAAGGGGGA AGGTGTGGCC TACCGAGGTC GGGTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TATTCCCTGT TTGCTGCCTT TTACTCGGCC 
TCCATGCTCC AGGATGTTGA TGATGCCATC CAGTTTGAGG TCAGCATCGG GAACTACGGT 
AACAAGTTCG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCTTACT GGGAGGACAT CAGCCACCGG GTCGAGGCTC AGAACCAGCT GCTCAGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCAGC 
CCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC CGACTGCAGC 
CAGCCCCTAG GCGACGTCCA AGAGATACCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
AAGCTGCGTA CCCGTCACCT AGGCCAGATC ACTGAGGCGG CCCTGGCACT GAAACTTGGC 
CGCAGCGAGC TCCCTGCTGC CCTGGAGCAG GCGGAAGACT GGCTGCTGCG TGTCCGGGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGAGTGG CGTACCAGCG GGTGCCCGCC CACGAGATCC TCTTCTCCCG GCGGGGTCCC 
AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATAT TTCTGAAATA TCCAATGGAG 
GGCGTTCCTG GAGCCCGGAT GCCAGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCCGTCTT TGCTGAAACG 
TATGAGAACC AGACCAAACT GGCCCTGGTT GGGAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGAAAGATT AAGCTACCCA AAGATAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATTGACGCGG GACACCTGAG TTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCCCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCCCCAT CCCCCCGGAT 
CGGAAGCCAA AGCACTGGGT TCCTGCTGAG AAGATGTACT ACACGCACCG TCGACGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACGGATGC CTTCCGCCGT CGCCGCTGGC GTCGCCGCAT GGAGCCCCTG 
GAGAAGACAG GGCCTGCGGC CGTGTTTGCC CTCGAGGGGG CTCTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATTA TGGGAACCGC TACCACCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGCG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTATGAGAT TGAGATCTTT GGTGAGCCAG CCAGCATCGC CGAGCAACCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTACG GTGCCGACGA GTTCATGGGA 
CGCTGCATCT GTCAGCCAAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGTTGATT 
AGGGGCAGCC AGCCAGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCAGGAAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC GTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGT GCTCCAGCGT ACCGCCATAG AGATCCTGGC ATGGGGCCTG 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCTC CCAGCCTTGT GGTGGAGTGT 
GGAGGCCAGA CCGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTTGAC 
ATCTGTACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
GTCCGCTCCC TGGAGAGCTT CATGTGTGAC CAGTACTCTG AAGAGAGTCC GTCCCCGCAG 
GGCGGCCCAG ATGACGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GATAAGGAGC CCCTCATCCC CATCCAGGAG GAGGAGTTCA TCGACTGGTG GAGCAAATTC 
TTTGCCTCCA CAGGGGAGAG GGAAAAGTGT GGCCAGTACT TGGAGAAGGG CTTTGACACT 
CTAAAGGTCT ATGACACACA ACTGGAGAAT GTGGAAGCCT TCGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACCGAAGA CCCATCTGTT 
ATTGGGGAGT TCAAGGGCCT CTTCAAAATC TATCCCCTCC CAGAGGACCC TGCCATTCCC 
ATGCCCCCAA GACAGTTCCA CCAACTGGCT GCCCAGGGGC CTCAGGAGTG CCTGGTCCGC 
ATCTACATCA TCCGAGCATT CGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATTCCTTGT 
ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AAGATGAAAA GATTGGGGAG 
ACAGTCATCG ACCTGGAGAA TAGGCTGCTG TCCAAGTTTG GAGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAAGCTCCTG TGTACCACAC AGACCGAGTG 
GTGTTTGAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAAGGT CCTGAACCCA 
CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGTATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAGCATG TGGAGTCCCG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAACAGGGG 
AAGCTGCAGA TGTGGATCGA CCTATTTCCA AAGGCCCTGG GGAGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGCT GTATTATCTG GAACACAAAG 
GACGTGATCC TGGACGACCT CAGCATCACA GGGGAGAAGA TGAGCGATAT TTACGTGAAA 
GGTTGGATGG TTGGCTTTGA AGAACACAAG CAAAAGACAG ATGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCAGCTGAG 
CAAGTCTGCA CTGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC AAGTCATATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT 
CTAGGCTCCT TGCAGCTGGA CCTCAACCAC ATGCCCAAAC CTGCCAAGAC AGCTGAGAAA 
TGCTCCTTGG ACCAGCTGGA TGGCACCTTC CACCCAGAGT GGTTTGTGTC CCTTTTTGAA 
CAGAAAACTG TGAAGGGCTG GTGGCCCTGT GTGGCAGAAG AGGGCGAGAA GAAGATCCTG 
GCGGGCAAAC TGGAAATGAC CTTGGAGATC GTAGCAGAGA ATGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA ACCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCT 
GATACCTCCT TCCTCTGGTT CACCTCTCCG TACAAGACTA TGAAGTTCAT CCTATGGCGG 
CGCTTCCGGT GTGCCATTAT TCTCTTCATC ATTCTCTTCA TCCTACTGCT GTTCCTGGGC 
ATCTTCGTCT ATGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984156.1
CDS	408..6707
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984107.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984107.4

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT TGGGCCCTGC 
TATGTCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCAGAA 
CTGAACATGG GCAAGGGGGA AGGTGTGGCC TACCGAGGTC GGGTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TATTCCCTGT TTGCTGCCTT TTACTCGGCC 
TCCATGCTCC AGGATGTTGA TGATGCCATC CAGTTTGAGG TCAGCATCGG GAACTACGGT 
AACAAGTTCG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCTTACT GGGAGGACAT CAGCCACCGG GTCGAGGCTC AGAACCAGCT GCTCAGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCAGC 
CCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC CGACTGCAGC 
CAGCCCCTAG GCGACGTCCA AGAGATACCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
AAGCTGCGTA CCCGTCACCT AGGCCAGATC ACTGAGGCGG CCCTGGCACT GAAACTTGGC 
CGCAGCGAGC TCCCTGCTGC CCTGGAGCAG GCGGAAGACT GGCTGCTGCG TGTCCGGGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGAGTGG CGTACCAGCG GGTGCCCGCC CACGAGATCC TCTTCTCCCG GCGGGGTCCC 
AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATAT TTCTGAAATA TCCAATGGAG 
GGCGTTCCTG GAGCCCGGAT GCCAGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCCGTCTT TGCTGAAACG 
TATGAGAACC AGACCAAACT GGCCCTGGTT GGGAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGAAAGATT AAGCTACCCA AAGATAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATTGACGCGG GACACCTGAG TTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCCCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCCCCAT CCCCCCGGAT 
CGGAAGCCAA AGCACTGGGT TCCTGCTGAG AAGATGTACT ACACGCACCG TCGACGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACGGATGC CTTCCGCCGT CGCCGCTGGC GTCGCCGCAT GGAGCCCCTG 
GAGAAGACAG GGCCTGCGGC CGTGTTTGCC CTCGAGGGGG CTCTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATTA TGGGAACCGC TACCACCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGCG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTATGAGAT TGAGATCTTT GGTGAGCCAG CCAGCATCGC CGAGCAACCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTACG GTGCCGACGA GTTCATGGGA 
CGCTGCATCT GTCAGCCAAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGTTGATT 
AGGGGCAGCC AGCCAGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCAGGAAT CCTGGAAGAG 
TCGGAGGACA CGGACCTGCC GTACCCACCG CCTCAGAGGG AGGCCAACAT CTACATGGTT 
CCCCAGAACA TCAAGCCAGT GCTCCAGCGT ACCGCCATAG AGATCCTGGC ATGGGGCCTG 
CGGAACATGA AGAGTTACCA GCTGGCCAGT ATCTCCTCTC CCAGCCTTGT GGTGGAGTGT 
GGAGGCCAGA CCGTGCAGTC CTGTGTCATC AAGAACCTCC GGAAGAACCC CAACTTTGAC 
ATCTGTACCC TCTTCATGGA AGTGATGCTG CCCAGGGAGG AGCTCTACTG TCCCCCCATC 
GTGATCAAGG TCATTGATAA CCGCCAGTTT GGCCGCCGGC CTGTGGTGGG CCAGTGTACC 
GTCCGCTCCC TGGAGAGCTT CATGTGTGAC CAGTACTCTG AAGAGAGTCC GTCCCCGCAG 
GGCGGCCCAG ATGACGTGAG TTTACTCAGT CCTGGGGAAG ACGTGCTCAT TGACATTGAT 
GATAAGGAGC CCCTCATCCC CATCCAGGAG GAGGAGTTCA TCGACTGGTG GAGCAAATTC 
TTTGCCTCCA CAGGGGAGAG GGAAAAGTGT GGCCAGTACT TGGAGAAGGG CTTTGACACT 
CTAAAGGTCT ATGACACACA ACTGGAGAAT GTGGAAGCCT TCGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACCGAAGA CCCATCTGTT 
ATTGGGGAGT TCAAGGGCCT CTTCAAAATC TATCCCCTCC CAGAGGACCC TGCCATTCCC 
ATGCCCCCAA GACAGTTCCA CCAACTGGCT GCCCAGGGGC CTCAGGAGTG CCTGGTCCGC 
ATCTACATCA TCCGAGCATT CGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATTCCTTGT 
ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACTCTCTA TGACTATGAC CTCCTCTCCA AAGATGAAAA GATTGGGGAG 
ACAGTCATCG ACCTGGAGAA TAGGCTGCTG TCCAAGTTTG GAGCTCGCTG TGGACTCCCA 
CAGACCTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAAGCTCCTG TGTACCACAC AGACCGAGTG 
GTGTTTGAGG ATAAAGAATA CACCATTGAG GAAATAGAGG CTGGCAAGGT CCTGAACCCA 
CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGTATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAGCATG TGGAGTCCCG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAACAGGGG 
AAGCTGCAGA TGTGGATCGA CCTATTTCCA AAGGCCCTGG GGAGGCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGCT GTATTATCTG GAACACAAAG 
GACGTGATCC TGGACGACCT CAGCATCACA GGGGAGAAGA TGAGCGATAT TTACGTGAAA 
GGTTGGATGG TTGGCTTTGA AGAACACAAG CAAAAGACAG ATGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCAGCTGAG 
CAAGTCTGCA CTGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC AAGTCATATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT 
CTAGGCTCCT TGCAGCTGGA CCTCAACCAC ATGCCCAAAC CTGCCAAGAC AGCTGAGAAA 
TGCTCCTTGG ACCAGCTGGA TGGCACCTTC CACCCAGAGT GGTTTGTGTC CCTTTTTGAA 
CAGAAAACTG TGAAGGGCTG GTGGCCCTGT GTGGCAGAAG AGGGCGAGAA GAAGATCCTG 
GCGGGCAAAC TGGAAATGAC CTTGGAGATC GTAGCAGAGA ATGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA ACCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCT 
GATACCTCCT TCCTCTGGTT CACCTCTCCG TACAAGACTA TGAAGTTCAT CCTATGGCGG 
CGCTTCCGGT GTGCCATTAT TCTCTTCATC ATTCTCTTCA TCCTACTGCT GTTCCTGGGC 
ATCTTCGTCT ATGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984111.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984160.1 Felis catus dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OFb04566
Clone ID Related Accession (Same CDS sequence)	XM_003984111.4
Accession Version	XM_003984111.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984111.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTTGG GCCCTGCTAT GTCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCAGAACTG AACATGGGCA AGGGGGAAGG TGTGGCCTAC 
CGAGGTCGGG TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAT 
TCCCTGTTTG CTGCCTTTTA CTCGGCCTCC ATGCTCCAGG ATGTTGATGA TGCCATCCAG 
TTTGAGGTCA GCATCGGGAA CTACGGTAAC AAGTTCGACA CGACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCTTACTGGG AGGACATCAG CCACCGGGTC 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCT GACCGGCTGG AAGCTGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCAGCCCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCCGA CTGCAGCCAG CCCCTAGGCG ACGTCCAAGA GATACCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACAAG CTGCGTACCC GTCACCTAGG CCAGATCACT 
GAGGCGGCCC TGGCACTGAA ACTTGGCCGC AGCGAGCTCC CTGCTGCCCT GGAGCAGGCG 
GAAGACTGGC TGCTGCGTGT CCGGGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGAGACAAG CGAGTGGCGT ACCAGCGGGT GCCCGCCCAC 
GAGATCCTCT TCTCCCGGCG GGGTCCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG 
ACGATATTTC TGAAATATCC AATGGAGGGC GTTCCTGGAG CCCGGATGCC AGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CCGTCTTTGC TGAAACGTAT GAGAACCAGA CCAAACTGGC CCTGGTTGGG 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG AAAGATTAAG 
CTACCCAAAG ATAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGACATT GACGCGGGAC ACCTGAGTTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC CCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCCCCATCCC CCCGGATCGG AAGCCAAAGC ACTGGGTTCC TGCTGAGAAG 
ATGTACTACA CGCACCGTCG ACGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CGGATGCCTT CCGCCGTCGC 
CGCTGGCGTC GCCGCATGGA GCCCCTGGAG AAGACAGGGC CTGCGGCCGT GTTTGCCCTC 
GAGGGGGCTC TGGGCGGCGT GGTGGACGAC AAGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGATTATGG GAACCGCTAC 
CACCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGCGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATTGA GATCTTTGGT 
GAGCCAGCCA GCATCGCCGA GCAACCGCCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGTG CCGACGAGTT CATGGGACGC TGCATCTGTC AGCCAAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GTTGATTAGG GGCAGCCAGC CAGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CAGGAATCCT GGAAGAGTCG GAGGACACGG ACCTGCCGTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGTGCT CCAGCGTACC 
GCCATAGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCTCCCA GCCTTGTGGT GGAGTGTGGA GGCCAGACCG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTTGACATC TGTACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCGTC CGCTCCCTGG AGAGCTTCAT GTGTGACCAG 
TACTCTGAAG AGAGTCCGTC CCCGCAGGGC GGCCCAGATG ACGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAT AAGGAGCCCC TCATCCCCAT CCAGCTTGCA 
GATGGTCTGT CGGGCTCTGC CCCCACTAAC ATGGCATCCT CTCCATCCAG TCCTCATGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGT 
GGCCAGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT 
GTGGAAGCCT TCGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACCGAAGA CCCATCTGTT ATTGGGGAGT TCAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAGGACCC TGCCATTCCC ATGCCCCCAA GACAGTTCCA CCAACTGGCT 
GCCCAGGGGC CTCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGAGCATT CGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCTTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GAGCTCGCTG TGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCGCAGAGTC 
AAAGCTCCTG TGTACCACAC AGACCGAGTG GTGTTTGAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAAGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGTATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCCCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCA 
AAGGCCCTGG GGAGGCCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGCT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGCGATAT TTACGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTGCATTA TCGCTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCTGAG CAAGTCTGCA CTGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTCATATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTAGGCTCCT TGCAGCTGGA CCTCAACCAC 
ATGCCCAAAC CTGCCAAGAC AGCTGAGAAA TGCTCCTTGG ACCAGCTGGA TGGCACCTTC 
CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG AGGGCGAGAA GAAGATCCTG GCGGGCAAAC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA ATGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCTAAGC TTGAGGACCC AAGGCGCCCT GATACCTCCT TCCTCTGGTT CACCTCTCCG 
TACAAGACTA TGAAGTTCAT CCTATGGCGG CGCTTCCGGT GTGCCATTAT TCTCTTCATC 
ATTCTCTTCA TCCTACTGCT GTTCCTGGGC ATCTTCGTCT ATGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984160.1
CDS	408..6677
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLADTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984111.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984111.4

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTTGG GCCCTGCTAT GTCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCAGAACTG AACATGGGCA AGGGGGAAGG TGTGGCCTAC 
CGAGGTCGGG TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAT 
TCCCTGTTTG CTGCCTTTTA CTCGGCCTCC ATGCTCCAGG ATGTTGATGA TGCCATCCAG 
TTTGAGGTCA GCATCGGGAA CTACGGTAAC AAGTTCGACA CGACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCTTACTGGG AGGACATCAG CCACCGGGTC 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCT GACCGGCTGG AAGCTGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCAGCCCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCCGA CTGCAGCCAG CCCCTAGGCG ACGTCCAAGA GATACCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACAAG CTGCGTACCC GTCACCTAGG CCAGATCACT 
GAGGCGGCCC TGGCACTGAA ACTTGGCCGC AGCGAGCTCC CTGCTGCCCT GGAGCAGGCG 
GAAGACTGGC TGCTGCGTGT CCGGGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGAGACAAG CGAGTGGCGT ACCAGCGGGT GCCCGCCCAC 
GAGATCCTCT TCTCCCGGCG GGGTCCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG 
ACGATATTTC TGAAATATCC AATGGAGGGC GTTCCTGGAG CCCGGATGCC AGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CCGTCTTTGC TGAAACGTAT GAGAACCAGA CCAAACTGGC CCTGGTTGGG 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG AAAGATTAAG 
CTACCCAAAG ATAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGACATT GACGCGGGAC ACCTGAGTTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC CCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCCCCATCCC CCCGGATCGG AAGCCAAAGC ACTGGGTTCC TGCTGAGAAG 
ATGTACTACA CGCACCGTCG ACGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CGGATGCCTT CCGCCGTCGC 
CGCTGGCGTC GCCGCATGGA GCCCCTGGAG AAGACAGGGC CTGCGGCCGT GTTTGCCCTC 
GAGGGGGCTC TGGGCGGCGT GGTGGACGAC AAGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGATTATGG GAACCGCTAC 
CACCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGCGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATTGA GATCTTTGGT 
GAGCCAGCCA GCATCGCCGA GCAACCGCCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGTG CCGACGAGTT CATGGGACGC TGCATCTGTC AGCCAAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GTTGATTAGG GGCAGCCAGC CAGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CAGGAATCCT GGAAGAGTCG GAGGACACGG ACCTGCCGTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGTGCT CCAGCGTACC 
GCCATAGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCTCCCA GCCTTGTGGT GGAGTGTGGA GGCCAGACCG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTTGACATC TGTACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCGTC CGCTCCCTGG AGAGCTTCAT GTGTGACCAG 
TACTCTGAAG AGAGTCCGTC CCCGCAGGGC GGCCCAGATG ACGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAT AAGGAGCCCC TCATCCCCAT CCAGCTTGCA 
GATGGTCTGT CGGGCTCTGC CCCCACTAAC ATGGCATCCT CTCCATCCAG TCCTCATGAG 
GAGGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGT 
GGCCAGTACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAT 
GTGGAAGCCT TCGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACCGAAGA CCCATCTGTT ATTGGGGAGT TCAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAGGACCC TGCCATTCCC ATGCCCCCAA GACAGTTCCA CCAACTGGCT 
GCCCAGGGGC CTCAGGAGTG CCTGGTCCGC ATCTACATCA TCCGAGCATT CGGCCTGCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCTTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACTCTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATCG ACCTGGAGAA TAGGCTGCTG 
TCCAAGTTTG GAGCTCGCTG TGGACTCCCA CAGACCTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTC CTCCACCTCT TCTGCCAGCA GCGCAGAGTC 
AAAGCTCCTG TGTACCACAC AGACCGAGTG GTGTTTGAGG ATAAAGAATA CACCATTGAG 
GAAATAGAGG CTGGCAAGGT CCTGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGTATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCCCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCA 
AAGGCCCTGG GGAGGCCTGG ACCTCCCTTC AACATCACCC CACGGAGAGC CAGAAGGTTT 
TTCCTGCGCT GTATTATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGCGATAT TTACGTGAAA GGTTGGATGG TTGGCTTTGA AGAACACAAG 
CAAAAGACAG ATGTGCATTA TCGCTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCTGAG CAAGTCTGCA CTGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC AAGTCATATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTAGGCTCCT TGCAGCTGGA CCTCAACCAC 
ATGCCCAAAC CTGCCAAGAC AGCTGAGAAA TGCTCCTTGG ACCAGCTGGA TGGCACCTTC 
CACCCAGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACTG TGAAGGGCTG GTGGCCCTGT 
GTGGCAGAAG AGGGCGAGAA GAAGATCCTG GCGGGCAAAC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA ATGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCTAAGC TTGAGGACCC AAGGCGCCCT GATACCTCCT TCCTCTGGTT CACCTCTCCG 
TACAAGACTA TGAAGTTCAT CCTATGGCGG CGCTTCCGGT GTGCCATTAT TCTCTTCATC 
ATTCTCTTCA TCCTACTGCT GTTCCTGGGC ATCTTCGTCT ATGCCTTCCC GAACTATGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984108.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984157.1 Felis catus dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OFb04567
Clone ID Related Accession (Same CDS sequence)	XM_003984108.4
Accession Version	XM_003984108.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984108.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCG TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGTG CTCCAGCGTA CCGCCATAGA GATCCTGGCA 
TGGGGCCTGC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCTCC CAGCCTTGTG 
GTGGAGTGTG GAGGCCAGAC CGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTTGACA TCTGTACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCG TCCGCTCCCT GGAGAGCTTC ATGTGTGACC AGTACTCTGA AGAGAGTCCG 
TCCCCGCAGG GCGGCCCAGA TGACGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ATAAGGAGCC CCTCATCCCC ATCCAGGAGG AGGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGTG GCCAGTACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAATG TGGAAGCCTT CGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACCGAAGAC 
CCATCTGTTA TTGGGGAGTT CAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAGGACCCT 
GCCATTCCCA TGCCCCCAAG ACAGTTCCAC CAACTGGCTG CCCAGGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTACATCAT CCGAGCATTC GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCTTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATCGA CCTGGAGAAT AGGCTGCTGT CCAAGTTTGG AGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AAGCTCCTGT GTACCACACA 
GACCGAGTGG TGTTTGAGGA TAAAGAATAC ACCATTGAGG AAATAGAGGC TGGCAAGGTC 
CTGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGTATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCAA AGGCCCTGGG GAGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGCTG TATTATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGCGATATT 
TACGTGAAAG GTTGGATGGT TGGCTTTGAA GAACACAAGC AAAAGACAGA TGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCTGAGC AAGTCTGCAC TGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACA AGTCATATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TAGGCTCCTT GCAGCTGGAC CTCAACCACA TGCCCAAACC TGCCAAGACA 
GCTGAGAAAT GCTCCTTGGA CCAGCTGGAT GGCACCTTCC ACCCAGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACTGT GAAGGGCTGG TGGCCCTGTG TGGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CGGGCAAACT GGAAATGACC TTGGAGATCG TAGCAGAGAA TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCTG ATACCTCCTT CCTCTGGTTC ACCTCTCCGT ACAAGACTAT GAAGTTCATC 
CTATGGCGGC GCTTCCGGTG TGCCATTATT CTCTTCATCA TTCTCTTCAT CCTACTGCTG 
TTCCTGGGCA TCTTCGTCTA TGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984157.1
CDS	408..6656
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLADTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984108.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984108.4

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCG TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGTG CTCCAGCGTA CCGCCATAGA GATCCTGGCA 
TGGGGCCTGC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCTCC CAGCCTTGTG 
GTGGAGTGTG GAGGCCAGAC CGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTTGACA TCTGTACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCG TCCGCTCCCT GGAGAGCTTC ATGTGTGACC AGTACTCTGA AGAGAGTCCG 
TCCCCGCAGG GCGGCCCAGA TGACGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ATAAGGAGCC CCTCATCCCC ATCCAGGAGG AGGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGTG GCCAGTACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAATG TGGAAGCCTT CGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACCGAAGAC 
CCATCTGTTA TTGGGGAGTT CAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAGGACCCT 
GCCATTCCCA TGCCCCCAAG ACAGTTCCAC CAACTGGCTG CCCAGGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTACATCAT CCGAGCATTC GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCTTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACTCTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATCGA CCTGGAGAAT AGGCTGCTGT CCAAGTTTGG AGCTCGCTGT 
GGACTCCCAC AGACCTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AAGCTCCTGT GTACCACACA 
GACCGAGTGG TGTTTGAGGA TAAAGAATAC ACCATTGAGG AAATAGAGGC TGGCAAGGTC 
CTGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGTATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCAA AGGCCCTGGG GAGGCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGCTG TATTATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGCGATATT 
TACGTGAAAG GTTGGATGGT TGGCTTTGAA GAACACAAGC AAAAGACAGA TGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCTGAGC AAGTCTGCAC TGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACA AGTCATATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TAGGCTCCTT GCAGCTGGAC CTCAACCACA TGCCCAAACC TGCCAAGACA 
GCTGAGAAAT GCTCCTTGGA CCAGCTGGAT GGCACCTTCC ACCCAGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACTGT GAAGGGCTGG TGGCCCTGTG TGGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CGGGCAAACT GGAAATGACC TTGGAGATCG TAGCAGAGAA TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCTG ATACCTCCTT CCTCTGGTTC ACCTCTCCGT ACAAGACTAT GAAGTTCATC 
CTATGGCGGC GCTTCCGGTG TGCCATTATT CTCTTCATCA TTCTCTTCAT CCTACTGCTG 
TTCCTGGGCA TCTTCGTCTA TGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984109.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984158.1 Felis catus dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OFb04568
Clone ID Related Accession (Same CDS sequence)	XM_003984109.4
Accession Version	XM_003984109.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003984109.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTTGG GCCCTGCTAT GTCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCAGAACTG AACATGGGCA AGGGGGAAGG TGTGGCCTAC 
CGAGGTCGGG TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAT 
TCCCTGTTTG CTGCCTTTTA CTCGGCCTCC ATGCTCCAGG ATGTTGATGA TGCCATCCAG 
TTTGAGGTCA GCATCGGGAA CTACGGTAAC AAGTTCGACA CGACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCTTACTGGG AGGACATCAG CCACCGGGTC 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCT GACCGGCTGG AAGCTGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCAGCCCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCCGA CTGCAGCCAG CCCCTAGGCG ACGTCCAAGA GATACCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACAAG CTGCGTACCC GTCACCTAGG CCAGATCACT 
GAGGCGGCCC TGGCACTGAA ACTTGGCCGC AGCGAGCTCC CTGCTGCCCT GGAGCAGGCG 
GAAGACTGGC TGCTGCGTGT CCGGGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGAGACAAG CGAGTGGCGT ACCAGCGGGT GCCCGCCCAC 
GAGATCCTCT TCTCCCGGCG GGGTCCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG 
ACGATATTTC TGAAATATCC AATGGAGGGC GTTCCTGGAG CCCGGATGCC AGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CCGTCTTTGC TGAAACGTAT GAGAACCAGA CCAAACTGGC CCTGGTTGGG 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG AAAGATTAAG 
CTACCCAAAG ATAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGACATT GACGCGGGAC ACCTGAGTTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC CCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCCCCATCCC CCCGGATCGG AAGCCAAAGC ACTGGGTTCC TGCTGAGAAG 
ATGTACTACA CGCACCGTCG ACGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CGGATGCCTT CCGCCGTCGC 
CGCTGGCGTC GCCGCATGGA GCCCCTGGAG AAGACAGGGC CTGCGGCCGT GTTTGCCCTC 
GAGGGGGCTC TGGGCGGCGT GGTGGACGAC AAGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGATTATGG GAACCGCTAC 
CACCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGCGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATTGA GATCTTTGGT 
GAGCCAGCCA GCATCGCCGA GCAACCGCCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGTG CCGACGAGTT CATGGGACGC TGCATCTGTC AGCCAAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GTTGATTAGG GGCAGCCAGC CAGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CAGGAATCCT GGAAGAGTCG GAGGACACGG ACCTGCCGTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGTGCT CCAGCGTACC 
GCCATAGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCTCCCA GCCTTGTGGT GGAGTGTGGA GGCCAGACCG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTTGACATC TGTACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCGTC CGCTCCCTGG AGAGCTTCAT GTGTGACCAG 
TACTCTGAAG AGAGTCCGTC CCCGCAGGGC GGCCCAGATG ACGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAT AAGGAGCCCC TCATCCCCAT CCAGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA AAAGTGTGGC 
CAGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG 
GAAGCCTTCG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CCGAAGACCC ATCTGTTATT GGGGAGTTCA AGGGCCTCTT CAAAATCTAT 
CCCCTCCCAG AGGACCCTGC CATTCCCATG CCCCCAAGAC AGTTCCACCA ACTGGCTGCC 
CAGGGGCCTC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTCGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT TCCTTGTACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAAG ATGAAAAGAT TGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGAG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAA 
GCTCCTGTGT ACCACACAGA CCGAGTGGTG TTTGAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAAGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAGCATGTGG AGTCCCGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA ACAGGGGAAG CTGCAGATGT GGATCGACCT ATTTCCAAAG 
GCCCTGGGGA GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGCTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACAGGG 
GAGAAGATGA GCGATATTTA CGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC AGCTGAGCAA GTCTGCACTG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TCATATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTA GGCTCCTTGC AGCTGGACCT CAACCACATG 
CCCAAACCTG CCAAGACAGC TGAGAAATGC TCCTTGGACC AGCTGGATGG CACCTTCCAC 
CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGAGG GCGAGAAGAA GATCCTGGCG GGCAAACTGG AAATGACCTT GGAGATCGTA 
GCAGAGAATG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAACC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAT ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC 
AAGACTATGA AGTTCATCCT ATGGCGGCGC TTCCGGTGTG CCATTATTCT CTTCATCATT 
CTCTTCATCC TACTGCTGTT CCTGGGCATC TTCGTCTATG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984158.1
CDS	408..6614
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLADTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984109.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984109.4

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA CGACCATCTG 
GGCTTCCTCC CCACCTTTGG GCCCTGCTAT GTCAACCTCT ATGGCAGCCC CAGGGAGTTC 
ACTGGCTTCC CGGACCCCTA CGCAGAACTG AACATGGGCA AGGGGGAAGG TGTGGCCTAC 
CGAGGTCGGG TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGA GCAGAAGGTG 
GAGGACCTCC CTGCGGATGA CATCCTTCGG GTGGAGAAGT ACCTCCGGAG GCGCAAGTAT 
TCCCTGTTTG CTGCCTTTTA CTCGGCCTCC ATGCTCCAGG ATGTTGATGA TGCCATCCAG 
TTTGAGGTCA GCATCGGGAA CTACGGTAAC AAGTTCGACA CGACCTGCCT GCCGCTGGCC 
TCCACCACCC AGTACAGCCG GGCGGTCTTT GACGGGTGCC ACTACTACTA CTTGCCCTGG 
GGCAACGTGA AGCCCGTGGT GGTGCTGTCA TCTTACTGGG AGGACATCAG CCACCGGGTC 
GAGGCTCAGA ACCAGCTGCT CAGGATTGCT GACCGGCTGG AAGCTGGCCT GGAGCAAGTC 
CACTTGGCCC TGAAGGCTCA GTGCAGCCCC GAGGACGTGG ACTCCCTGTT GGCTCAGCTG 
ATGGATGAGC TCATCTCCGA CTGCAGCCAG CCCCTAGGCG ACGTCCAAGA GATACCCTCT 
GCCACCCACC TGGACCAGTA CCTGTACAAG CTGCGTACCC GTCACCTAGG CCAGATCACT 
GAGGCGGCCC TGGCACTGAA ACTTGGCCGC AGCGAGCTCC CTGCTGCCCT GGAGCAGGCG 
GAAGACTGGC TGCTGCGTGT CCGGGCCCTG GCAGATGAGC CCCAGAACAG CCTGCCCGAC 
ATCATCATCT GGATGCTGCA GGGAGACAAG CGAGTGGCGT ACCAGCGGGT GCCCGCCCAC 
GAGATCCTCT TCTCCCGGCG GGGTCCCAAC TACTGTGGCA AGAATTGTGG GAAGCTGCAG 
ACGATATTTC TGAAATATCC AATGGAGGGC GTTCCTGGAG CCCGGATGCC AGTGCAGATA 
CGGGTGAAGC TCTGGTTTGG GCTTTCTGTG GATGAGAAGG AGTTCAACCA GTTTGCTGAG 
GGAAAGCTGT CCGTCTTTGC TGAAACGTAT GAGAACCAGA CCAAACTGGC CCTGGTTGGG 
AACTGGGGCA CAACAGGCCT CACCTACCCC AAGTTTTCTG ATGTCACGGG AAAGATTAAG 
CTACCCAAAG ATAGCTTCCG CCCCTCGGCC GGCTGGACCT GGGCTGGAGA CTGGTTCGTG 
TGTCCAGAGA AGACCCTGCT CCATGACATT GACGCGGGAC ACCTGAGTTT TGTGGAGGAG 
GTGTTTGAGA ACCAGACCCG GCTCCCTGGA GGCCAGTGGA TCTACATGAG TGACAACTAC 
ACGGATGTGA ATGGGGAGAA GGTGCTTCCC AAGGATGACA TTGAGTGCCC CCTGGGCTGG 
AAGTGGGAAG ATGAAGAGTG GTCCACGGAC CTCAATCGGG CCGTTGATGA GCAAGGCTGG 
GAGTACAGTA TCCCCATCCC CCCGGATCGG AAGCCAAAGC ACTGGGTTCC TGCTGAGAAG 
ATGTACTACA CGCACCGTCG ACGGCGCTGG GTCCGCCTGC GCAGGAGGGA CCTCAGCCAG 
ATGGAGGCAC TGAAGAGGCA CAGGCAGGCA GAGGCGGAGG GCGAGGGCTG GGAGTACGCC 
TCGCTCTTTG GCTGGAAGTT CCACCTGGAG TACCGCAAGA CGGATGCCTT CCGCCGTCGC 
CGCTGGCGTC GCCGCATGGA GCCCCTGGAG AAGACAGGGC CTGCGGCCGT GTTTGCCCTC 
GAGGGGGCTC TGGGCGGCGT GGTGGACGAC AAGAGTGAAG ATTCCATGTC CGTGTCCACC 
TTGAGCTTTG GTGTGAACAG ACCCACGATT TCCTGCATCT TTGATTATGG GAACCGCTAC 
CACCTACGCT GCTACATGTA CCAGGCCCGG GACCTGCCCT CGATGGACAA GGACTCTTTT 
TCTGATCCCT ACGCCATCGT CTCCTTCCTG CACCAGAGCC AGAAGACCGT GGTGGCGAAG 
AACACTCTGA ACCCCACCTG GGACCAGACA CTCATCTTCT ATGAGATTGA GATCTTTGGT 
GAGCCAGCCA GCATCGCCGA GCAACCGCCC AGCATTGTGG TGGAGCTGTA TGACCATGAC 
ACCTACGGTG CCGACGAGTT CATGGGACGC TGCATCTGTC AGCCAAGTCT GGAACGGATG 
CCCCGGCTGG CCTGGTTCCC GTTGATTAGG GGCAGCCAGC CAGCGGGTGA GCTGCTGGCC 
TCTTTTGAGC TCATCCAGAG AGAGAAGCCG GCCATCCACC ATATTCCTGG TTTTGAGGTG 
CAGGATACAT CAGGAATCCT GGAAGAGTCG GAGGACACGG ACCTGCCGTA CCCACCGCCT 
CAGAGGGAGG CCAACATCTA CATGGTTCCC CAGAACATCA AGCCAGTGCT CCAGCGTACC 
GCCATAGAGA TCCTGGCATG GGGCCTGCGG AACATGAAGA GTTACCAGCT GGCCAGTATC 
TCCTCTCCCA GCCTTGTGGT GGAGTGTGGA GGCCAGACCG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTTGACATC TGTACCCTCT TCATGGAAGT GATGCTGCCC 
AGGGAGGAGC TCTACTGTCC CCCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCGTC CGCTCCCTGG AGAGCTTCAT GTGTGACCAG 
TACTCTGAAG AGAGTCCGTC CCCGCAGGGC GGCCCAGATG ACGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATTGATGAT AAGGAGCCCC TCATCCCCAT CCAGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA AAAGTGTGGC 
CAGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG 
GAAGCCTTCG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CCGAAGACCC ATCTGTTATT GGGGAGTTCA AGGGCCTCTT CAAAATCTAT 
CCCCTCCCAG AGGACCCTGC CATTCCCATG CCCCCAAGAC AGTTCCACCA ACTGGCTGCC 
CAGGGGCCTC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTCGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT TCCTTGTACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAAG ATGAAAAGAT TGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGAG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAA 
GCTCCTGTGT ACCACACAGA CCGAGTGGTG TTTGAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAAGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAGCATGTGG AGTCCCGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA ACAGGGGAAG CTGCAGATGT GGATCGACCT ATTTCCAAAG 
GCCCTGGGGA GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGCTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACAGGG 
GAGAAGATGA GCGATATTTA CGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC AGCTGAGCAA GTCTGCACTG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TCATATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTA GGCTCCTTGC AGCTGGACCT CAACCACATG 
CCCAAACCTG CCAAGACAGC TGAGAAATGC TCCTTGGACC AGCTGGATGG CACCTTCCAC 
CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGAGG GCGAGAAGAA GATCCTGGCG GGCAAACTGG AAATGACCTT GGAGATCGTA 
GCAGAGAATG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAACC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAT ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC 
AAGACTATGA AGTTCATCCT ATGGCGGCGC TTCCGGTGTG CCATTATTCT CTTCATCATT 
CTCTTCATCC TACTGCTGTT CCTGGGCATC TTCGTCTATG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019827392.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682951.1 Felis catus dysferlin (DYSF), transcript variant X1, mRNA.

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CloneID	OFb40430
Clone ID Related Accession (Same CDS sequence)	XM_019827392.1
Accession Version	XM_019827392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCC TCATAAGCCC 
CTCCTGGAGG AGGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG 
GAAAAGTGTG GCCAGTACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA 
CTGGAGAATG TGGAAGCCTT CGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA GACCGAAGAC CCATCTGTTA TTGGGGAGTT CAAGGGCCTC 
TTCAAAATCT ATCCCCTCCC AGAGGACCCT GCCATTCCCA TGCCCCCAAG ACAGTTCCAC 
CAACTGGCTG CCCAGGGGCC TCAGGAGTGC CTGGTCCGCA TCTACATCAT CCGAGCATTC 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCTTGTA CTCTGGAGCC TGTGTTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATCGA CCTGGAGAAT 
AGGCTGCTGT CCAAGTTTGG AGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AAGCTCCTGT GTACCACACA GACCGAGTGG TGTTTGAGGA TAAAGAATAC 
ACCATTGAGG AAATAGAGGC TGGCAAGGTC CTGAACCCAC ACCTGGGCCC AGTGGAAGAG 
CGTCTGGCCT TGTATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC 
CTATTTCCAA AGGCCCTGGG GAGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGCTG TATTATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC 
AGCATCACAG GGGAGAAGAT GAGCGATATT TACGTGAAAG GTTGGATGGT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA TGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTTAAC 
TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCTGAGC AAGTCTGCAC TGTCTCTAAG 
AAGGATGCTT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACA AGTCATATTT 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GACGACTTTC TAGGCTCCTT GCAGCTGGAC 
CTCAACCACA TGCCCAAACC TGCCAAGACA GCTGAGAAAT GCTCCTTGGA CCAGCTGGAT 
GGCACCTTCC ACCCAGAGTG GTTTGTGTCC CTTTTTGAAC AGAAAACTGT GAAGGGCTGG 
TGGCCCTGTG TGGCAGAAGA GGGCGAGAAG AAGATCCTGG CGGGCAAACT GGAAATGACC 
TTGGAGATCG TAGCAGAGAA TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAA 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ATACCTCCTT CCTCTGGTTC 
ACCTCTCCGT ACAAGACTAT GAAGTTCATC CTATGGCGGC GCTTCCGGTG TGCCATTATT 
CTCTTCATCA TTCTCTTCAT CCTACTGCTG TTCCTGGGCA TCTTCGTCTA TGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682951.1
CDS	409..6867
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHKPLLEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019827392.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827392.1

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCGGAG GACACGGACC TGCCGTACCC ACCGCCTCAG 
AGGGAGGCCA ACATCTACAT GGTTCCCCAG AACATCAAGC CAGTGCTCCA GCGTACCGCC 
ATAGAGATCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGTATCTCC 
TCTCCCAGCC TTGTGGTGGA GTGTGGAGGC CAGACCGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGTCCCCC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCGTCCGC TCCCTGGAGA GCTTCATGTG TGACCAGTAC 
TCTGAAGAGA GTCCGTCCCC GCAGGGCGGC CCAGATGACG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT TGATGATAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCCTCTC CATCCAGTCC TCATAAGCCC 
CTCCTGGAGG AGGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG 
GAAAAGTGTG GCCAGTACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA 
CTGGAGAATG TGGAAGCCTT CGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA GACCGAAGAC CCATCTGTTA TTGGGGAGTT CAAGGGCCTC 
TTCAAAATCT ATCCCCTCCC AGAGGACCCT GCCATTCCCA TGCCCCCAAG ACAGTTCCAC 
CAACTGGCTG CCCAGGGGCC TCAGGAGTGC CTGGTCCGCA TCTACATCAT CCGAGCATTC 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCTTGTA CTCTGGAGCC TGTGTTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACTCTCTAT 
GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATCGA CCTGGAGAAT 
AGGCTGCTGT CCAAGTTTGG AGCTCGCTGT GGACTCCCAC AGACCTACTG TGTCTCTGGA 
CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AAGCTCCTGT GTACCACACA GACCGAGTGG TGTTTGAGGA TAAAGAATAC 
ACCATTGAGG AAATAGAGGC TGGCAAGGTC CTGAACCCAC ACCTGGGCCC AGTGGAAGAG 
CGTCTGGCCT TGTATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC 
CTATTTCCAA AGGCCCTGGG GAGGCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGCTG TATTATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC 
AGCATCACAG GGGAGAAGAT GAGCGATATT TACGTGAAAG GTTGGATGGT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA TGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTTAAC 
TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCTGAGC AAGTCTGCAC TGTCTCTAAG 
AAGGATGCTT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACA AGTCATATTT 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GACGACTTTC TAGGCTCCTT GCAGCTGGAC 
CTCAACCACA TGCCCAAACC TGCCAAGACA GCTGAGAAAT GCTCCTTGGA CCAGCTGGAT 
GGCACCTTCC ACCCAGAGTG GTTTGTGTCC CTTTTTGAAC AGAAAACTGT GAAGGGCTGG 
TGGCCCTGTG TGGCAGAAGA GGGCGAGAAG AAGATCCTGG CGGGCAAACT GGAAATGACC 
TTGGAGATCG TAGCAGAGAA TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAA 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCTG ATACCTCCTT CCTCTGGTTC 
ACCTCTCCGT ACAAGACTAT GAAGTTCATC CTATGGCGGC GCTTCCGGTG TGCCATTATT 
CTCTTCATCA TTCTCTTCAT CCTACTGCTG TTCCTGGGCA TCTTCGTCTA TGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019827393.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682952.1 Felis catus dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OFb40431
Clone ID Related Accession (Same CDS sequence)	XM_019827393.1
Accession Version	XM_019827393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGC TTGCAGATGG TCTGTCGGGC TCTGCCCCCA CTAACATGGC ATCCTCTCCA 
TCCAGTCCTC ATAAGCCCCT CCTGGAGGAG GAGTTCATCG ACTGGTGGAG CAAATTCTTT 
GCCTCCACAG GGGAGAGGGA AAAGTGTGGC CAGTACTTGG AGAAGGGCTT TGACACTCTA 
AAGGTCTATG ACACACAACT GGAGAATGTG GAAGCCTTCG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA CCGAAGACCC ATCTGTTATT 
GGGGAGTTCA AGGGCCTCTT CAAAATCTAT CCCCTCCCAG AGGACCCTGC CATTCCCATG 
CCCCCAAGAC AGTTCCACCA ACTGGCTGCC CAGGGGCCTC AGGAGTGCCT GGTCCGCATC 
TACATCATCC GAGCATTCGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT TCCTTGTACT 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAAG ATGAAAAGAT TGGGGAGACA 
GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTTGGAG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC CAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAA GCTCCTGTGT ACCACACAGA CCGAGTGGTG 
TTTGAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAAGGTCCT GAACCCACAC 
CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG TATGTCCTCC AGCAGCAGGG CCTGGTCCCT 
GAGCATGTGG AGTCCCGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA ACAGGGGAAG 
CTGCAGATGT GGATCGACCT ATTTCCAAAG GCCCTGGGGA GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGCTGTA TTATCTGGAA CACAAAGGAC 
GTGATCCTGG ACGACCTCAG CATCACAGGG GAGAAGATGA GCGATATTTA CGTGAAAGGT 
TGGATGGTTG GCTTTGAAGA ACACAAGCAA AAGACAGATG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCC AGCTGAGCAA 
GTCTGCACTG TCTCTAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TCATATTTCA GATCTGGGAC AATGACAAGT TCTCCTTTGA CGACTTTCTA 
GGCTCCTTGC AGCTGGACCT CAACCACATG CCCAAACCTG CCAAGACAGC TGAGAAATGC 
TCCTTGGACC AGCTGGATGG CACCTTCCAC CCAGAGTGGT TTGTGTCCCT TTTTGAACAG 
AAAACTGTGA AGGGCTGGTG GCCCTGTGTG GCAGAAGAGG GCGAGAAGAA GATCCTGGCG 
GGCAAACTGG AAATGACCTT GGAGATCGTA GCAGAGAATG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAACC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAT 
ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC AAGACTATGA AGTTCATCCT ATGGCGGCGC 
TTCCGGTGTG CCATTATTCT CTTCATCATT CTCTTCATCC TACTGCTGTT CCTGGGCATC 
TTCGTCTATG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682952.1
CDS	409..6825
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHKPLLEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019827393.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827393.1

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGC TTGCAGATGG TCTGTCGGGC TCTGCCCCCA CTAACATGGC ATCCTCTCCA 
TCCAGTCCTC ATAAGCCCCT CCTGGAGGAG GAGTTCATCG ACTGGTGGAG CAAATTCTTT 
GCCTCCACAG GGGAGAGGGA AAAGTGTGGC CAGTACTTGG AGAAGGGCTT TGACACTCTA 
AAGGTCTATG ACACACAACT GGAGAATGTG GAAGCCTTCG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA CCGAAGACCC ATCTGTTATT 
GGGGAGTTCA AGGGCCTCTT CAAAATCTAT CCCCTCCCAG AGGACCCTGC CATTCCCATG 
CCCCCAAGAC AGTTCCACCA ACTGGCTGCC CAGGGGCCTC AGGAGTGCCT GGTCCGCATC 
TACATCATCC GAGCATTCGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT TCCTTGTACT 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAAG ATGAAAAGAT TGGGGAGACA 
GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTTGGAG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC CAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAA GCTCCTGTGT ACCACACAGA CCGAGTGGTG 
TTTGAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAAGGTCCT GAACCCACAC 
CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG TATGTCCTCC AGCAGCAGGG CCTGGTCCCT 
GAGCATGTGG AGTCCCGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA ACAGGGGAAG 
CTGCAGATGT GGATCGACCT ATTTCCAAAG GCCCTGGGGA GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGCTGTA TTATCTGGAA CACAAAGGAC 
GTGATCCTGG ACGACCTCAG CATCACAGGG GAGAAGATGA GCGATATTTA CGTGAAAGGT 
TGGATGGTTG GCTTTGAAGA ACACAAGCAA AAGACAGATG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCC AGCTGAGCAA 
GTCTGCACTG TCTCTAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TCATATTTCA GATCTGGGAC AATGACAAGT TCTCCTTTGA CGACTTTCTA 
GGCTCCTTGC AGCTGGACCT CAACCACATG CCCAAACCTG CCAAGACAGC TGAGAAATGC 
TCCTTGGACC AGCTGGATGG CACCTTCCAC CCAGAGTGGT TTGTGTCCCT TTTTGAACAG 
AAAACTGTGA AGGGCTGGTG GCCCTGTGTG GCAGAAGAGG GCGAGAAGAA GATCCTGGCG 
GGCAAACTGG AAATGACCTT GGAGATCGTA GCAGAGAATG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAACC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAT 
ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC AAGACTATGA AGTTCATCCT ATGGCGGCGC 
TTCCGGTGTG CCATTATTCT CTTCATCATT CTCTTCATCC TACTGCTGTT CCTGGGCATC 
TTCGTCTATG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019827394.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682953.1 Felis catus dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OFb40432
Clone ID Related Accession (Same CDS sequence)	XM_019827394.1
Accession Version	XM_019827394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT TGGGCCCTGC 
TATGTCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCAGAA 
CTGAACATGG GCAAGGGGGA AGGTGTGGCC TACCGAGGTC GGGTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TATTCCCTGT TTGCTGCCTT TTACTCGGCC 
TCCATGCTCC AGGATGTTGA TGATGCCATC CAGTTTGAGG TCAGCATCGG GAACTACGGT 
AACAAGTTCG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCTTACT GGGAGGACAT CAGCCACCGG GTCGAGGCTC AGAACCAGCT GCTCAGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCAGC 
CCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC CGACTGCAGC 
CAGCCCCTAG GCGACGTCCA AGAGATACCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
AAGCTGCGTA CCCGTCACCT AGGCCAGATC ACTGAGGCGG CCCTGGCACT GAAACTTGGC 
CGCAGCGAGC TCCCTGCTGC CCTGGAGCAG GCGGAAGACT GGCTGCTGCG TGTCCGGGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGAGTGG CGTACCAGCG GGTGCCCGCC CACGAGATCC TCTTCTCCCG GCGGGGTCCC 
AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATAT TTCTGAAATA TCCAATGGAG 
GGCGTTCCTG GAGCCCGGAT GCCAGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCCGTCTT TGCTGAAACG 
TATGAGAACC AGACCAAACT GGCCCTGGTT GGGAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGAAAGATT AAGCTACCCA AAGATAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATTGACGCGG GACACCTGAG TTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCCCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCCCCAT CCCCCCGGAT 
CGGAAGCCAA AGCACTGGGT TCCTGCTGAG AAGATGTACT ACACGCACCG TCGACGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACGGATGC CTTCCGCCGT CGCCGCTGGC GTCGCCGCAT GGAGCCCCTG 
GAGAAGACAG GGCCTGCGGC CGTGTTTGCC CTCGAGGGGG CTCTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATTA TGGGAACCGC TACCACCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGCG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTATGAGAT TGAGATCTTT GGTGAGCCAG CCAGCATCGC CGAGCAACCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTACG GTGCCGACGA GTTCATGGGA 
CGCTGCATCT GTCAGCCAAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGTTGATT 
AGGGGCAGCC AGCCAGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCAGGAAT CCTGGAAGAG 
CTGTGTGCAC CTGCCTTCTT CCTCCAGGGC CTCCTCCAAA CGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGC TTGCAGATGG TCTGTCGGGC TCTGCCCCCA CTAACATGGC ATCCTCTCCA 
TCCAGTCCTC ATAAGCCCCT CCTGGAGGAG GAGTTCATCG ACTGGTGGAG CAAATTCTTT 
GCCTCCACAG GGGAGAGGGA AAAGTGTGGC CAGTACTTGG AGAAGGGCTT TGACACTCTA 
AAGGTCTATG ACACACAACT GGAGAATGTG GAAGCCTTCG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA CCGAAGACCC ATCTGTTATT 
GGGGAGTTCA AGGGCCTCTT CAAAATCTAT CCCCTCCCAG AGGACCCTGC CATTCCCATG 
CCCCCAAGAC AGTTCCACCA ACTGGCTGCC CAGGGGCCTC AGGAGTGCCT GGTCCGCATC 
TACATCATCC GAGCATTCGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT TCCTTGTACT 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAAG ATGAAAAGAT TGGGGAGACA 
GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTTGGAG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC CAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAA GCTCCTGTGT ACCACACAGA CCGAGTGGTG 
TTTGAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAAGGTCCT GAACCCACAC 
CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG TATGTCCTCC AGCAGCAGGG CCTGGTCCCT 
GAGCATGTGG AGTCCCGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA ACAGGGGAAG 
CTGCAGATGT GGATCGACCT ATTTCCAAAG GCCCTGGGGA GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGCTGTA TTATCTGGAA CACAAAGGAC 
GTGATCCTGG ACGACCTCAG CATCACAGGG GAGAAGATGA GCGATATTTA CGTGAAAGGT 
TGGATGGTTG GCTTTGAAGA ACACAAGCAA AAGACAGATG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCC AGCTGAGCAA 
GTCTGCACTG TCTCTAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TCATATTTCA GATCTGGGAC AATGACAAGT TCTCCTTTGA CGACTTTCTA 
GGCTCCTTGC AGCTGGACCT CAACCACATG CCCAAACCTG CCAAGACAGC TGAGAAATGC 
TCCTTGGACC AGCTGGATGG CACCTTCCAC CCAGAGTGGT TTGTGTCCCT TTTTGAACAG 
AAAACTGTGA AGGGCTGGTG GCCCTGTGTG GCAGAAGAGG GCGAGAAGAA GATCCTGGCG 
GGCAAACTGG AAATGACCTT GGAGATCGTA GCAGAGAATG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAACC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAT 
ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC AAGACTATGA AGTTCATCCT ATGGCGGCGC 
TTCCGGTGTG CCATTATTCT CTTCATCATT CTCTTCATCC TACTGCTGTT CCTGGGCATC 
TTCGTCTATG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682953.1
CDS	409..6825
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHKPLLEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019827394.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827394.1

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGACGACCAT CTGGGCTTCC TCCCCACCTT TGGGCCCTGC 
TATGTCAACC TCTATGGCAG CCCCAGGGAG TTCACTGGCT TCCCGGACCC CTACGCAGAA 
CTGAACATGG GCAAGGGGGA AGGTGTGGCC TACCGAGGTC GGGTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGAGCAGAAG GTGGAGGACC TCCCTGCGGA TGACATCCTT 
CGGGTGGAGA AGTACCTCCG GAGGCGCAAG TATTCCCTGT TTGCTGCCTT TTACTCGGCC 
TCCATGCTCC AGGATGTTGA TGATGCCATC CAGTTTGAGG TCAGCATCGG GAACTACGGT 
AACAAGTTCG ACACGACCTG CCTGCCGCTG GCCTCCACCA CCCAGTACAG CCGGGCGGTC 
TTTGACGGGT GCCACTACTA CTACTTGCCC TGGGGCAACG TGAAGCCCGT GGTGGTGCTG 
TCATCTTACT GGGAGGACAT CAGCCACCGG GTCGAGGCTC AGAACCAGCT GCTCAGGATT 
GCTGACCGGC TGGAAGCTGG CCTGGAGCAA GTCCACTTGG CCCTGAAGGC TCAGTGCAGC 
CCCGAGGACG TGGACTCCCT GTTGGCTCAG CTGATGGATG AGCTCATCTC CGACTGCAGC 
CAGCCCCTAG GCGACGTCCA AGAGATACCC TCTGCCACCC ACCTGGACCA GTACCTGTAC 
AAGCTGCGTA CCCGTCACCT AGGCCAGATC ACTGAGGCGG CCCTGGCACT GAAACTTGGC 
CGCAGCGAGC TCCCTGCTGC CCTGGAGCAG GCGGAAGACT GGCTGCTGCG TGTCCGGGCC 
CTGGCAGATG AGCCCCAGAA CAGCCTGCCC GACATCATCA TCTGGATGCT GCAGGGAGAC 
AAGCGAGTGG CGTACCAGCG GGTGCCCGCC CACGAGATCC TCTTCTCCCG GCGGGGTCCC 
AACTACTGTG GCAAGAATTG TGGGAAGCTG CAGACGATAT TTCTGAAATA TCCAATGGAG 
GGCGTTCCTG GAGCCCGGAT GCCAGTGCAG ATACGGGTGA AGCTCTGGTT TGGGCTTTCT 
GTGGATGAGA AGGAGTTCAA CCAGTTTGCT GAGGGAAAGC TGTCCGTCTT TGCTGAAACG 
TATGAGAACC AGACCAAACT GGCCCTGGTT GGGAACTGGG GCACAACAGG CCTCACCTAC 
CCCAAGTTTT CTGATGTCAC GGGAAAGATT AAGCTACCCA AAGATAGCTT CCGCCCCTCG 
GCCGGCTGGA CCTGGGCTGG AGACTGGTTC GTGTGTCCAG AGAAGACCCT GCTCCATGAC 
ATTGACGCGG GACACCTGAG TTTTGTGGAG GAGGTGTTTG AGAACCAGAC CCGGCTCCCT 
GGAGGCCAGT GGATCTACAT GAGTGACAAC TACACGGATG TGAATGGGGA GAAGGTGCTT 
CCCAAGGATG ACATTGAGTG CCCCCTGGGC TGGAAGTGGG AAGATGAAGA GTGGTCCACG 
GACCTCAATC GGGCCGTTGA TGAGCAAGGC TGGGAGTACA GTATCCCCAT CCCCCCGGAT 
CGGAAGCCAA AGCACTGGGT TCCTGCTGAG AAGATGTACT ACACGCACCG TCGACGGCGC 
TGGGTCCGCC TGCGCAGGAG GGACCTCAGC CAGATGGAGG CACTGAAGAG GCACAGGCAG 
GCAGAGGCGG AGGGCGAGGG CTGGGAGTAC GCCTCGCTCT TTGGCTGGAA GTTCCACCTG 
GAGTACCGCA AGACGGATGC CTTCCGCCGT CGCCGCTGGC GTCGCCGCAT GGAGCCCCTG 
GAGAAGACAG GGCCTGCGGC CGTGTTTGCC CTCGAGGGGG CTCTGGGCGG CGTGGTGGAC 
GACAAGAGTG AAGATTCCAT GTCCGTGTCC ACCTTGAGCT TTGGTGTGAA CAGACCCACG 
ATTTCCTGCA TCTTTGATTA TGGGAACCGC TACCACCTAC GCTGCTACAT GTACCAGGCC 
CGGGACCTGC CCTCGATGGA CAAGGACTCT TTTTCTGATC CCTACGCCAT CGTCTCCTTC 
CTGCACCAGA GCCAGAAGAC CGTGGTGGCG AAGAACACTC TGAACCCCAC CTGGGACCAG 
ACACTCATCT TCTATGAGAT TGAGATCTTT GGTGAGCCAG CCAGCATCGC CGAGCAACCG 
CCCAGCATTG TGGTGGAGCT GTATGACCAT GACACCTACG GTGCCGACGA GTTCATGGGA 
CGCTGCATCT GTCAGCCAAG TCTGGAACGG ATGCCCCGGC TGGCCTGGTT CCCGTTGATT 
AGGGGCAGCC AGCCAGCGGG TGAGCTGCTG GCCTCTTTTG AGCTCATCCA GAGAGAGAAG 
CCGGCCATCC ACCATATTCC TGGTTTTGAG GTGCAGGATA CATCAGGAAT CCTGGAAGAG 
CTGTGTGCAC CTGCCTTCTT CCTCCAGGGC CTCCTCCAAA CGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGC TTGCAGATGG TCTGTCGGGC TCTGCCCCCA CTAACATGGC ATCCTCTCCA 
TCCAGTCCTC ATAAGCCCCT CCTGGAGGAG GAGTTCATCG ACTGGTGGAG CAAATTCTTT 
GCCTCCACAG GGGAGAGGGA AAAGTGTGGC CAGTACTTGG AGAAGGGCTT TGACACTCTA 
AAGGTCTATG ACACACAACT GGAGAATGTG GAAGCCTTCG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTCTACCG GGGCAAGACT CAGGAGGAGA CCGAAGACCC ATCTGTTATT 
GGGGAGTTCA AGGGCCTCTT CAAAATCTAT CCCCTCCCAG AGGACCCTGC CATTCCCATG 
CCCCCAAGAC AGTTCCACCA ACTGGCTGCC CAGGGGCCTC AGGAGTGCCT GGTCCGCATC 
TACATCATCC GAGCATTCGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT TCCTTGTACT 
CTGGAGCCTG TGTTTGGAAA GATGTTCGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGATCA CTCTCTATGA CTATGACCTC CTCTCCAAAG ATGAAAAGAT TGGGGAGACA 
GTCATCGACC TGGAGAATAG GCTGCTGTCC AAGTTTGGAG CTCGCTGTGG ACTCCCACAG 
ACCTACTGTG TCTCTGGACC CAACCAGTGG AGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAA GCTCCTGTGT ACCACACAGA CCGAGTGGTG 
TTTGAGGATA AAGAATACAC CATTGAGGAA ATAGAGGCTG GCAAGGTCCT GAACCCACAC 
CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG TATGTCCTCC AGCAGCAGGG CCTGGTCCCT 
GAGCATGTGG AGTCCCGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA ACAGGGGAAG 
CTGCAGATGT GGATCGACCT ATTTCCAAAG GCCCTGGGGA GGCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGCTGTA TTATCTGGAA CACAAAGGAC 
GTGATCCTGG ACGACCTCAG CATCACAGGG GAGAAGATGA GCGATATTTA CGTGAAAGGT 
TGGATGGTTG GCTTTGAAGA ACACAAGCAA AAGACAGATG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTTAACTG GAGGTTCATT TTCCCCTTTG ACTACCTGCC AGCTGAGCAA 
GTCTGCACTG TCTCTAAGAA GGATGCTTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TCATATTTCA GATCTGGGAC AATGACAAGT TCTCCTTTGA CGACTTTCTA 
GGCTCCTTGC AGCTGGACCT CAACCACATG CCCAAACCTG CCAAGACAGC TGAGAAATGC 
TCCTTGGACC AGCTGGATGG CACCTTCCAC CCAGAGTGGT TTGTGTCCCT TTTTGAACAG 
AAAACTGTGA AGGGCTGGTG GCCCTGTGTG GCAGAAGAGG GCGAGAAGAA GATCCTGGCG 
GGCAAACTGG AAATGACCTT GGAGATCGTA GCAGAGAATG AGCATGAGGA GCGGCCTGCT 
GGCCAGGGCC GGGATGAACC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCTGAT 
ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC AAGACTATGA AGTTCATCCT ATGGCGGCGC 
TTCCGGTGTG CCATTATTCT CTTCATCATT CTCTTCATCC TACTGCTGTT CCTGGGCATC 
TTCGTCTATG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019827395.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682954.1 Felis catus dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OFb40433
Clone ID Related Accession (Same CDS sequence)	XM_019827395.1
Accession Version	XM_019827395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGTCGGAGGA CACGGACCTG CCGTACCCAC CGCCTCAGAG GGAGGCCAAC 
ATCTACATGG TTCCCCAGAA CATCAAGCCA GTGCTCCAGC GTACCGCCAT AGAGATCCTG 
GCATGGGGCC TGCGGAACAT GAAGAGTTAC CAGCTGGCCA GTATCTCCTC TCCCAGCCTT 
GTGGTGGAGT GTGGAGGCCA GACCGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTTG ACATCTGTAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTCTAC 
TGTCCCCCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC TTCATGTGTG ACCAGTACTC TGAAGAGAGT 
CCGTCCCCGC AGGGCGGCCC AGATGACGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATTG ATGATAAGGA GCCCCTCATC CCCATCCAGC TTGCAGATGG TCTGTCGGGC 
TCTGCCCCCA CTAACATGGC ATCCTCTCCA TCCAGTCCTC ATAAGCCCCT CCTGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA AAAGTGTGGC 
CAGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG 
GAAGCCTTCG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CCGAAGACCC ATCTGTTATT GGGGAGTTCA AGGGCCTCTT CAAAATCTAT 
CCCCTCCCAG AGGACCCTGC CATTCCCATG CCCCCAAGAC AGTTCCACCA ACTGGCTGCC 
CAGGGGCCTC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTCGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT TCCTTGTACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAAG ATGAAAAGAT TGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGAG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAA 
GCTCCTGTGT ACCACACAGA CCGAGTGGTG TTTGAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAAGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAGCATGTGG AGTCCCGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA ACAGGGGAAG CTGCAGATGT GGATCGACCT ATTTCCAAAG 
GCCCTGGGGA GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGCTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACAGGG 
GAGAAGATGA GCGATATTTA CGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC AGCTGAGCAA GTCTGCACTG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TCATATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTA GGCTCCTTGC AGCTGGACCT CAACCACATG 
CCCAAACCTG CCAAGACAGC TGAGAAATGC TCCTTGGACC AGCTGGATGG CACCTTCCAC 
CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGAGG GCGAGAAGAA GATCCTGGCG GGCAAACTGG AAATGACCTT GGAGATCGTA 
GCAGAGAATG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAACC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAT ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC 
AAGACTATGA AGTTCATCCT ATGGCGGCGC TTCCGGTGTG CCATTATTCT CTTCATCATT 
CTCTTCATCC TACTGCTGTT CCTGGGCATC TTCGTCTATG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682954.1
CDS	409..6795
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHKPLLEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019827395.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827395.1

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGCGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGTCGGAGGA CACGGACCTG CCGTACCCAC CGCCTCAGAG GGAGGCCAAC 
ATCTACATGG TTCCCCAGAA CATCAAGCCA GTGCTCCAGC GTACCGCCAT AGAGATCCTG 
GCATGGGGCC TGCGGAACAT GAAGAGTTAC CAGCTGGCCA GTATCTCCTC TCCCAGCCTT 
GTGGTGGAGT GTGGAGGCCA GACCGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTTG ACATCTGTAC CCTCTTCATG GAAGTGATGC TGCCCAGGGA GGAGCTCTAC 
TGTCCCCCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC TTCATGTGTG ACCAGTACTC TGAAGAGAGT 
CCGTCCCCGC AGGGCGGCCC AGATGACGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATTG ATGATAAGGA GCCCCTCATC CCCATCCAGC TTGCAGATGG TCTGTCGGGC 
TCTGCCCCCA CTAACATGGC ATCCTCTCCA TCCAGTCCTC ATAAGCCCCT CCTGGAGGAG 
GAGTTCATCG ACTGGTGGAG CAAATTCTTT GCCTCCACAG GGGAGAGGGA AAAGTGTGGC 
CAGTACTTGG AGAAGGGCTT TGACACTCTA AAGGTCTATG ACACACAACT GGAGAATGTG 
GAAGCCTTCG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTCTACCG GGGCAAGACT 
CAGGAGGAGA CCGAAGACCC ATCTGTTATT GGGGAGTTCA AGGGCCTCTT CAAAATCTAT 
CCCCTCCCAG AGGACCCTGC CATTCCCATG CCCCCAAGAC AGTTCCACCA ACTGGCTGCC 
CAGGGGCCTC AGGAGTGCCT GGTCCGCATC TACATCATCC GAGCATTCGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT TCCTTGTACT CTGGAGCCTG TGTTTGGAAA GATGTTCGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGATCA CTCTCTATGA CTATGACCTC 
CTCTCCAAAG ATGAAAAGAT TGGGGAGACA GTCATCGACC TGGAGAATAG GCTGCTGTCC 
AAGTTTGGAG CTCGCTGTGG ACTCCCACAG ACCTACTGTG TCTCTGGACC CAACCAGTGG 
AGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAA 
GCTCCTGTGT ACCACACAGA CCGAGTGGTG TTTGAGGATA AAGAATACAC CATTGAGGAA 
ATAGAGGCTG GCAAGGTCCT GAACCCACAC CTGGGCCCAG TGGAAGAGCG TCTGGCCTTG 
TATGTCCTCC AGCAGCAGGG CCTGGTCCCT GAGCATGTGG AGTCCCGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA ACAGGGGAAG CTGCAGATGT GGATCGACCT ATTTCCAAAG 
GCCCTGGGGA GGCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGCTGTA TTATCTGGAA CACAAAGGAC GTGATCCTGG ACGACCTCAG CATCACAGGG 
GAGAAGATGA GCGATATTTA CGTGAAAGGT TGGATGGTTG GCTTTGAAGA ACACAAGCAA 
AAGACAGATG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTTAACTG GAGGTTCATT 
TTCCCCTTTG ACTACCTGCC AGCTGAGCAA GTCTGCACTG TCTCTAAGAA GGATGCTTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TCATATTTCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA CGACTTTCTA GGCTCCTTGC AGCTGGACCT CAACCACATG 
CCCAAACCTG CCAAGACAGC TGAGAAATGC TCCTTGGACC AGCTGGATGG CACCTTCCAC 
CCAGAGTGGT TTGTGTCCCT TTTTGAACAG AAAACTGTGA AGGGCTGGTG GCCCTGTGTG 
GCAGAAGAGG GCGAGAAGAA GATCCTGGCG GGCAAACTGG AAATGACCTT GGAGATCGTA 
GCAGAGAATG AGCATGAGGA GCGGCCTGCT GGCCAGGGCC GGGATGAACC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCTGAT ACCTCCTTCC TCTGGTTCAC CTCTCCGTAC 
AAGACTATGA AGTTCATCCT ATGGCGGCGC TTCCGGTGTG CCATTATTCT CTTCATCATT 
CTCTTCATCC TACTGCTGTT CCTGGGCATC TTCGTCTATG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019827396.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682955.1 Felis catus dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OFb40434
Clone ID Related Accession (Same CDS sequence)	XM_019827396.1
Accession Version	XM_019827396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTCCAAAC GTCGGAGGAC 
ACGGACCTGC CGTACCCACC GCCTCAGAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC 
ATCAAGCCAG TGCTCCAGCG TACCGCCATA GAGATCCTGG CATGGGGCCT GCGGAACATG 
AAGAGTTACC AGCTGGCCAG TATCTCCTCT CCCAGCCTTG TGGTGGAGTG TGGAGGCCAG 
ACCGTGCAGT CCTGTGTCAT CAAGAACCTC CGGAAGAACC CCAACTTTGA CATCTGTACC 
CTCTTCATGG AAGTGATGCT GCCCAGGGAG GAGCTCTACT GTCCCCCCAT CGTGATCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCTGTGGTGG GCCAGTGTAC CGTCCGCTCC 
CTGGAGAGCT TCATGTGTGA CCAGTACTCT GAAGAGAGTC CGTCCCCGCA GGGCGGCCCA 
GATGACGTGA GTTTACTCAG TCCTGGGGAA GACGTGCTCA TTGACATTGA TGATAAGGAG 
CCCCTCATCC CCATCCAGCT TGCAGATGGT CTGTCGGGCT CTGCCCCCAC TAACATGGCA 
TCCTCTCCAT CCAGTCCTCA TAAGCCCCTC CTGGAGGAGG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGTGGCC AGTACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAATGTGG AAGCCTTCGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC CGAAGACCCA 
TCTGTTATTG GGGAGTTCAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA GGACCCTGCC 
ATTCCCATGC CCCCAAGACA GTTCCACCAA CTGGCTGCCC AGGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ACATCATCCG AGCATTCGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCTTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATCGACCT GGAGAATAGG CTGCTGTCCA AGTTTGGAGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAAG CTCCTGTGTA CCACACAGAC 
CGAGTGGTGT TTGAGGATAA AGAATACACC ATTGAGGAAA TAGAGGCTGG CAAGGTCCTG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGT ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCAAAGG CCCTGGGGAG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGCTGTAT TATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG CGATATTTAC 
GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCTGAGCAAG TCTGCACTGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT CATATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTAG GCTCCTTGCA GCTGGACCTC AACCACATGC CCAAACCTGC CAAGACAGCT 
GAGAAATGCT CCTTGGACCA GCTGGATGGC ACCTTCCACC CAGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACTGTGAA GGGCTGGTGG CCCTGTGTGG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCGG GCAAACTGGA AATGACCTTG GAGATCGTAG CAGAGAATGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGATA CCTCCTTCCT CTGGTTCACC TCTCCGTACA AGACTATGAA GTTCATCCTA 
TGGCGGCGCT TCCGGTGTGC CATTATTCTC TTCATCATTC TCTTCATCCT ACTGCTGTTC 
CTGGGCATCT TCGTCTATGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682955.1
CDS	409..6774
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLADTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHKPLLEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019827396.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827396.1

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGC TGTGTGCACC TGCCTTCTTC CTCCAGGGCC TCCTCCAAAC GTCGGAGGAC 
ACGGACCTGC CGTACCCACC GCCTCAGAGG GAGGCCAACA TCTACATGGT TCCCCAGAAC 
ATCAAGCCAG TGCTCCAGCG TACCGCCATA GAGATCCTGG CATGGGGCCT GCGGAACATG 
AAGAGTTACC AGCTGGCCAG TATCTCCTCT CCCAGCCTTG TGGTGGAGTG TGGAGGCCAG 
ACCGTGCAGT CCTGTGTCAT CAAGAACCTC CGGAAGAACC CCAACTTTGA CATCTGTACC 
CTCTTCATGG AAGTGATGCT GCCCAGGGAG GAGCTCTACT GTCCCCCCAT CGTGATCAAG 
GTCATTGATA ACCGCCAGTT TGGCCGCCGG CCTGTGGTGG GCCAGTGTAC CGTCCGCTCC 
CTGGAGAGCT TCATGTGTGA CCAGTACTCT GAAGAGAGTC CGTCCCCGCA GGGCGGCCCA 
GATGACGTGA GTTTACTCAG TCCTGGGGAA GACGTGCTCA TTGACATTGA TGATAAGGAG 
CCCCTCATCC CCATCCAGCT TGCAGATGGT CTGTCGGGCT CTGCCCCCAC TAACATGGCA 
TCCTCTCCAT CCAGTCCTCA TAAGCCCCTC CTGGAGGAGG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGTGGCC AGTACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAATGTGG AAGCCTTCGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC CGAAGACCCA 
TCTGTTATTG GGGAGTTCAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA GGACCCTGCC 
ATTCCCATGC CCCCAAGACA GTTCCACCAA CTGGCTGCCC AGGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ACATCATCCG AGCATTCGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCTTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC TCTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATCGACCT GGAGAATAGG CTGCTGTCCA AGTTTGGAGC TCGCTGTGGA 
CTCCCACAGA CCTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAAG CTCCTGTGTA CCACACAGAC 
CGAGTGGTGT TTGAGGATAA AGAATACACC ATTGAGGAAA TAGAGGCTGG CAAGGTCCTG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGT ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCAAAGG CCCTGGGGAG GCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGCTGTAT TATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG CGATATTTAC 
GTGAAAGGTT GGATGGTTGG CTTTGAAGAA CACAAGCAAA AGACAGATGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCTGAGCAAG TCTGCACTGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT CATATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTAG GCTCCTTGCA GCTGGACCTC AACCACATGC CCAAACCTGC CAAGACAGCT 
GAGAAATGCT CCTTGGACCA GCTGGATGGC ACCTTCCACC CAGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACTGTGAA GGGCTGGTGG CCCTGTGTGG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCGG GCAAACTGGA AATGACCTTG GAGATCGTAG CAGAGAATGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCTGATA CCTCCTTCCT CTGGTTCACC TCTCCGTACA AGACTATGAA GTTCATCCTA 
TGGCGGCGCT TCCGGTGTGC CATTATTCTC TTCATCATTC TCTTCATCCT ACTGCTGTTC 
CTGGGCATCT TCGTCTATGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_019827397.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019682956.1 Felis catus dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OFb40435
Clone ID Related Accession (Same CDS sequence)	XM_019827397.1
Accession Version	XM_019827397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	dysferlin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCG TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGTG CTCCAGCGTA CCGCCATAGA GATCCTGGCA 
TGGGGCCTGC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCTCC CAGCCTTGTG 
GTGGAGTGTG GAGGCCAGAC CGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTTGACA TCTGTACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCG TCCGCTCCCT GGAGAGCTTC ATGTGTGACC AGTACTCTGA AGAGAGTCCG 
TCCCCGCAGG GCGGCCCAGA TGACGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ATAAGGAGCC CCTCATCCCC ATCCAGCTTG CAGATGGTCT GTCGGGCTCT 
GCCCCCACTA ACATGGCATC CTCTCCATCC AGTCCTCATA AGCCCCTCCT GGAGGAGGAG 
TTCATCGACT GGTGGAGCAA ATTCTTTGCC TCCACAGGGG AGAGGGAAAA GTGTGGCCAG 
TACTTGGAGA AGGGCTTTGA CACTCTAAAG GTCTATGACA CACAACTGGA GAATGTGGAA 
GCCTTCGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGAGACCG AAGACCCATC TGTTATTGGG GAGTTCAAGG GCCTCTTCAA AATCTATCCC 
CTCCCAGAGG ACCCTGCCAT TCCCATGCCC CCAAGACAGT TCCACCAACT GGCTGCCCAG 
GGGCCTCAGG AGTGCCTGGT CCGCATCTAC ATCATCCGAG CATTCGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATTCC TTGTACTCTG GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAAGATG AAAAGATTGG GGAGACAGTC ATCGACCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGAGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCCAA CCAGTGGAGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAAGCT 
CCTGTGTACC ACACAGACCG AGTGGTGTTT GAGGATAAAG AATACACCAT TGAGGAAATA 
GAGGCTGGCA AGGTCCTGAA CCCACACCTG GGCCCAGTGG AAGAGCGTCT GGCCTTGTAT 
GTCCTCCAGC AGCAGGGCCT GGTCCCTGAG CATGTGGAGT CCCGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAACA GGGGAAGCTG CAGATGTGGA TCGACCTATT TCCAAAGGCC 
CTGGGGAGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGCTGTATTA TCTGGAACAC AAAGGACGTG ATCCTGGACG ACCTCAGCAT CACAGGGGAG 
AAGATGAGCG ATATTTACGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCATTTTC 
CCCTTTGACT ACCTGCCAGC TGAGCAAGTC TGCACTGTCT CTAAGAAGGA TGCTTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTCA TATTTCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGACGA CTTTCTAGGC TCCTTGCAGC TGGACCTCAA CCACATGCCC 
AAACCTGCCA AGACAGCTGA GAAATGCTCC TTGGACCAGC TGGATGGCAC CTTCCACCCA 
GAGTGGTTTG TGTCCCTTTT TGAACAGAAA ACTGTGAAGG GCTGGTGGCC CTGTGTGGCA 
GAAGAGGGCG AGAAGAAGAT CCTGGCGGGC AAACTGGAAA TGACCTTGGA GATCGTAGCA 
GAGAATGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAACCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGATACC TCCTTCCTCT GGTTCACCTC TCCGTACAAG 
ACTATGAAGT TCATCCTATG GCGGCGCTTC CGGTGTGCCA TTATTCTCTT CATCATTCTC 
TTCATCCTAC TGCTGTTCCT GGGCATCTTC GTCTATGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019682956.1
CDS	409..6732
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLADTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSGSAPTNMASSPSSPHKPLLEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAENEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_019827397.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019827397.1

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGACA CAGGAGGAGA GGAGGACACT 
GAAGACCAGG GGCTCACAGG AGATGAGGCA GAGCCATTAG TGGATCAGAG TGGGGCCCCA 
GGCCCTGGGG CTCCCGCCAC CCCGAGGAAG CCACCTTCCC ATCCACCCCC CTACCACCCC 
GGGATCAAAA GGAAGAGAAG TGCTCCCACA TCCAGAAAGC TGCTGTCAGA CAAACCACAG 
GACTTCCAGA TCAGGGTGCA AGTGATCGAG GGGCGCCAGC TGCCAGGGGT GAACATCAAG 
CCTGTGGTCA AGGTCACTGC TGCCGGGCAG ACCAAGAGGA CACGGATCCA AAAGGGAAAC 
AGCCCACTCT TCAATGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCCTC GGAGCTGTTT 
GATGAGCCCA TCTTCATCAC GGTGGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACACGC CTATCTCAGG 
AAGTGGCTGC TACTCTCAGA CCCTGATGAT TTCTCTGCTG GGGCCAGAGG TTACCTGAAA 
GCCAGCCTTT GTGTACTGGG CCCCGGAGAC GAAGCACCGC TGGAGAGAAA GGACCCCTCT 
GAAGATAAGG AGGACATCGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTTCGAGGA 
GCCCACTTCT GCCTGAAGGT CTTCAGAGCT GAGGACTTAC CACAGATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGTAACA AGAAGAACTT GGTGGATCCC 
TTCATGGAAG TCAGCTTTGC AGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACCCTG CCTGCCATGT TTCCCTCTAT GTGTGAAAAA 
ATGAGGATTC GCGTCATAGA CTGGGACCGC CTCACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CATGGACCTG GACGACCATC TGGGCTTCCT CCCCACCTTT 
GGGCCCTGCT ATGTCAACCT CTATGGCAGC CCCAGGGAGT TCACTGGCTT CCCGGACCCC 
TACGCAGAAC TGAACATGGG CAAGGGGGAA GGTGTGGCCT ACCGAGGTCG GGTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GAGCAGAAGG TGGAGGACCT CCCTGCGGAT 
GACATCCTTC GGGTGGAGAA GTACCTCCGG AGGCGCAAGT ATTCCCTGTT TGCTGCCTTT 
TACTCGGCCT CCATGCTCCA GGATGTTGAT GATGCCATCC AGTTTGAGGT CAGCATCGGG 
AACTACGGTA ACAAGTTCGA CACGACCTGC CTGCCGCTGG CCTCCACCAC CCAGTACAGC 
CGGGCGGTCT TTGACGGGTG CCACTACTAC TACTTGCCCT GGGGCAACGT GAAGCCCGTG 
GTGGTGCTGT CATCTTACTG GGAGGACATC AGCCACCGGG TCGAGGCTCA GAACCAGCTG 
CTCAGGATTG CTGACCGGCT GGAAGCTGGC CTGGAGCAAG TCCACTTGGC CCTGAAGGCT 
CAGTGCAGCC CCGAGGACGT GGACTCCCTG TTGGCTCAGC TGATGGATGA GCTCATCTCC 
GACTGCAGCC AGCCCCTAGG CGACGTCCAA GAGATACCCT CTGCCACCCA CCTGGACCAG 
TACCTGTACA AGCTGCGTAC CCGTCACCTA GGCCAGATCA CTGAGGCGGC CCTGGCACTG 
AAACTTGGCC GCAGCGAGCT CCCTGCTGCC CTGGAGCAGG CGGAAGACTG GCTGCTGCGT 
GTCCGGGCCC TGGCAGATGA GCCCCAGAAC AGCCTGCCCG ACATCATCAT CTGGATGCTG 
CAGGGAGACA AGCGAGTGGC GTACCAGCGG GTGCCCGCCC ACGAGATCCT CTTCTCCCGG 
CGGGGTCCCA ACTACTGTGG CAAGAATTGT GGGAAGCTGC AGACGATATT TCTGAAATAT 
CCAATGGAGG GCGTTCCTGG AGCCCGGATG CCAGTGCAGA TACGGGTGAA GCTCTGGTTT 
GGGCTTTCTG TGGATGAGAA GGAGTTCAAC CAGTTTGCTG AGGGAAAGCT GTCCGTCTTT 
GCTGAAACGT ATGAGAACCA GACCAAACTG GCCCTGGTTG GGAACTGGGG CACAACAGGC 
CTCACCTACC CCAAGTTTTC TGATGTCACG GGAAAGATTA AGCTACCCAA AGATAGCTTC 
CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA GACTGGTTCG TGTGTCCAGA GAAGACCCTG 
CTCCATGACA TTGACGCGGG ACACCTGAGT TTTGTGGAGG AGGTGTTTGA GAACCAGACC 
CGGCTCCCTG GAGGCCAGTG GATCTACATG AGTGACAACT ACACGGATGT GAATGGGGAG 
AAGGTGCTTC CCAAGGATGA CATTGAGTGC CCCCTGGGCT GGAAGTGGGA AGATGAAGAG 
TGGTCCACGG ACCTCAATCG GGCCGTTGAT GAGCAAGGCT GGGAGTACAG TATCCCCATC 
CCCCCGGATC GGAAGCCAAA GCACTGGGTT CCTGCTGAGA AGATGTACTA CACGCACCGT 
CGACGGCGCT GGGTCCGCCT GCGCAGGAGG GACCTCAGCC AGATGGAGGC ACTGAAGAGG 
CACAGGCAGG CAGAGGCGGA GGGCGAGGGC TGGGAGTACG CCTCGCTCTT TGGCTGGAAG 
TTCCACCTGG AGTACCGCAA GACGGATGCC TTCCGCCGTC GCCGCTGGCG TCGCCGCATG 
GAGCCCCTGG AGAAGACAGG GCCTGCGGCC GTGTTTGCCC TCGAGGGGGC TCTGGGCGGC 
GTGGTGGACG ACAAGAGTGA AGATTCCATG TCCGTGTCCA CCTTGAGCTT TGGTGTGAAC 
AGACCCACGA TTTCCTGCAT CTTTGATTAT GGGAACCGCT ACCACCTACG CTGCTACATG 
TACCAGGCCC GGGACCTGCC CTCGATGGAC AAGGACTCTT TTTCTGATCC CTACGCCATC 
GTCTCCTTCC TGCACCAGAG CCAGAAGACC GTGGTGGCGA AGAACACTCT GAACCCCACC 
TGGGACCAGA CACTCATCTT CTATGAGATT GAGATCTTTG GTGAGCCAGC CAGCATCGCC 
GAGCAACCGC CCAGCATTGT GGTGGAGCTG TATGACCATG ACACCTACGG TGCCGACGAG 
TTCATGGGAC GCTGCATCTG TCAGCCAAGT CTGGAACGGA TGCCCCGGCT GGCCTGGTTC 
CCGTTGATTA GGGGCAGCCA GCCAGCGGGT GAGCTGCTGG CCTCTTTTGA GCTCATCCAG 
AGAGAGAAGC CGGCCATCCA CCATATTCCT GGTTTTGAGG TGCAGGATAC ATCAGGAATC 
CTGGAAGAGT CGGAGGACAC GGACCTGCCG TACCCACCGC CTCAGAGGGA GGCCAACATC 
TACATGGTTC CCCAGAACAT CAAGCCAGTG CTCCAGCGTA CCGCCATAGA GATCCTGGCA 
TGGGGCCTGC GGAACATGAA GAGTTACCAG CTGGCCAGTA TCTCCTCTCC CAGCCTTGTG 
GTGGAGTGTG GAGGCCAGAC CGTGCAGTCC TGTGTCATCA AGAACCTCCG GAAGAACCCC 
AACTTTGACA TCTGTACCCT CTTCATGGAA GTGATGCTGC CCAGGGAGGA GCTCTACTGT 
CCCCCCATCG TGATCAAGGT CATTGATAAC CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC 
CAGTGTACCG TCCGCTCCCT GGAGAGCTTC ATGTGTGACC AGTACTCTGA AGAGAGTCCG 
TCCCCGCAGG GCGGCCCAGA TGACGTGAGT TTACTCAGTC CTGGGGAAGA CGTGCTCATT 
GACATTGATG ATAAGGAGCC CCTCATCCCC ATCCAGCTTG CAGATGGTCT GTCGGGCTCT 
GCCCCCACTA ACATGGCATC CTCTCCATCC AGTCCTCATA AGCCCCTCCT GGAGGAGGAG 
TTCATCGACT GGTGGAGCAA ATTCTTTGCC TCCACAGGGG AGAGGGAAAA GTGTGGCCAG 
TACTTGGAGA AGGGCTTTGA CACTCTAAAG GTCTATGACA CACAACTGGA GAATGTGGAA 
GCCTTCGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TCTACCGGGG CAAGACTCAG 
GAGGAGACCG AAGACCCATC TGTTATTGGG GAGTTCAAGG GCCTCTTCAA AATCTATCCC 
CTCCCAGAGG ACCCTGCCAT TCCCATGCCC CCAAGACAGT TCCACCAACT GGCTGCCCAG 
GGGCCTCAGG AGTGCCTGGT CCGCATCTAC ATCATCCGAG CATTCGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATTCC TTGTACTCTG GAGCCTGTGT TTGGAAAGAT GTTCGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGATCACTC TCTATGACTA TGACCTCCTC 
TCCAAAGATG AAAAGATTGG GGAGACAGTC ATCGACCTGG AGAATAGGCT GCTGTCCAAG 
TTTGGAGCTC GCTGTGGACT CCCACAGACC TACTGTGTCT CTGGACCCAA CCAGTGGAGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAAGCT 
CCTGTGTACC ACACAGACCG AGTGGTGTTT GAGGATAAAG AATACACCAT TGAGGAAATA 
GAGGCTGGCA AGGTCCTGAA CCCACACCTG GGCCCAGTGG AAGAGCGTCT GGCCTTGTAT 
GTCCTCCAGC AGCAGGGCCT GGTCCCTGAG CATGTGGAGT CCCGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAACA GGGGAAGCTG CAGATGTGGA TCGACCTATT TCCAAAGGCC 
CTGGGGAGGC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGCTGTATTA TCTGGAACAC AAAGGACGTG ATCCTGGACG ACCTCAGCAT CACAGGGGAG 
AAGATGAGCG ATATTTACGT GAAAGGTTGG ATGGTTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGATGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TTAACTGGAG GTTCATTTTC 
CCCTTTGACT ACCTGCCAGC TGAGCAAGTC TGCACTGTCT CTAAGAAGGA TGCTTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTCA TATTTCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGACGA CTTTCTAGGC TCCTTGCAGC TGGACCTCAA CCACATGCCC 
AAACCTGCCA AGACAGCTGA GAAATGCTCC TTGGACCAGC TGGATGGCAC CTTCCACCCA 
GAGTGGTTTG TGTCCCTTTT TGAACAGAAA ACTGTGAAGG GCTGGTGGCC CTGTGTGGCA 
GAAGAGGGCG AGAAGAAGAT CCTGGCGGGC AAACTGGAAA TGACCTTGGA GATCGTAGCA 
GAGAATGAGC ATGAGGAGCG GCCTGCTGGC CAGGGCCGGG ATGAACCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCTGATACC TCCTTCCTCT GGTTCACCTC TCCGTACAAG 
ACTATGAAGT TCATCCTATG GCGGCGCTTC CGGTGTGCCA TTATTCTCTT CATCATTCTC 
TTCATCCTAC TGCTGTTCCT GGGCATCTTC GTCTATGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003984110.5 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003984159.2 Felis catus dysferlin (DYSF), mRNA.

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CloneID	OFb73655
Clone ID Related Accession (Same CDS sequence)	XM_003984110.5
Accession Version	XM_003984110.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	LOW QUALITY PROTEIN: dysferlin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018725.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_003984110.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGTGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGG AGGAGGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAAAAGT GTGGCCAGTA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ATGTGGAAGC CTTCGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACCGAA GACCCATCTG TTATTGGGGA GTTCAAGGGC 
CTCTTCAAAA TCTATCCCCT CCCAGAGGAC CCTGCCATTC CCATGCCCCC AAGACAGTTC 
CACCAACTGG CTGCCCAGGG GCCTCAGGAG TGCCTGGTCC GCATCTACAT CATCCGAGCA 
TTCGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCTT GTACTCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT CGACCTGGAG 
AATAGGCTGC TGTCCAAGTT TGGAGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAAGCTCC TGTGTACCAC ACAGACCGAG TGGTGTTTGA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAAG GTCCTGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGTATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC 
GACCTATTTC CAAAGGCCCT GGGGAGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG CTGTATTATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAT ATTTACGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCTG AGCAAGTCTG CACTGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTCATA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTAGGCTC CTTGCAGCTG 
GACCTCAACC ACATGCCCAA ACCTGCCAAG ACAGCTGAGA AATGCTCCTT GGACCAGCTG 
GATGGCACCT TCCACCCAGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC TGTGAAGGGC 
TGGTGGCCCT GTGTGGCAGA AGAGGGCGAG AAGAAGATCC TGGCGGGCAA ACTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAACCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CTGATACCTC CTTCCTCTGG 
TTCACCTCTC CGTACAAGAC TATGAAGTTC ATCCTATGGC GGCGCTTCCG GTGTGCCATT 
ATTCTCTTCA TCATTCTCTT CATCCTACTG CTGTTCCTGG GCATCTTCGT CTATGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003984159.2
CDS	410..6751
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEANIPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPSASLEPSPTLPDLDMLAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEAEPLVDQSGAPGPGAPATPRKPPSHPPPYHPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIQKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKASLCVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPAMDLDDHLGFLPTFGPCYVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRVLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSASMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADRLEAGLEQVHLALKAQCSPEDVDSLLAQLMDELISDCSQPLGDVQEIPSATHLDQYLYKLRTRHLGQITEAALALKLGRSELPAALEQAEDWLLRVRALADEPQNSLPDIIIWMLQGDKRVAYQRVPAHEILFSRRGPNYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDIDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSIPIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPLIRGSQPAGELLASFELIQREKPAIHHIPGFEVQDTSGILEESEDTDLPYPPPQREANIYMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPREELYCPPIVIKVIDNRQFGRRPVVGQCTVRSLESFMCDQYSEESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASTGEREKCGQYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLENRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYHTDRVVFEDKEYTIEEIEAGKVLNPHLGPVEERLALYVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDGTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFVYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003984110.5
Organism	Felis catus(domestic cat)
Definition	Felis catus dysferlin (DYSF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003984110.5

ORF Insert Sequence:

ATGTTGAGAG TCTTCATCCT CTACGCCGAG AACGTCCACA CCCCAGACAC CGATATCAGC 
GATGCCTACT GCTCAGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CCGTATGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCTTTGGAC 
CAGAGCTCTG AACTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGAAG GAACAGGTTC 
TTGGGGGAAG CCAACATCCC ACTCCGGGAG GTCCTTGCCA CCCCCAGTCT GTCTGCGAGC 
TTCAATGCTC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCTTGCAG 
GTGTCCTACA CGCCACTCCC TGGAGCTGTG CCCCTGTTCC CACCGTCTGC GTCTCTGGAA 
CCCTCCCCAA CTCTGCCGGA CCTGGATATG CTGGCTGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAAGACC AGGGGCTCAC AGGAGATGAG 
GCAGAGCCAT TAGTGGATCA GAGTGGGGCC CCAGGCCCTG GGGCTCCCGC CACCCCGAGG 
AAGCCACCTT CCCATCCACC CCCCTACCAC CCCGGGATCA AAAGGAAGAG AAGTGCTCCC 
ACATCCAGAA AGCTGCTGTC AGACAAACCA CAGGACTTCC AGATCAGGGT GCAAGTGATC 
GAGGGGCGCC AGCTGCCAGG GGTGAACATC AAGCCTGTGG TCAAGGTCAC TGCTGCCGGG 
CAGACCAAGA GGACACGGAT CCAAAAGGGA AACAGCCCAC TCTTCAATGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC CTCGGAGCTG TTTGATGAGC CCATCTTCAT CACGGTGGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA CGCCTATCTC AGGAAGTGGC TGCTACTCTC AGACCCTGAT 
GATTTCTCTG CTGGGGCCAG AGGTTACCTG AAAGCCAGCC TTTGTGTACT GGGCCCCGGA 
GACGAAGCAC CGCTGGAGAG AAAGGACCCC TCTGAAGATA AGGAGGACAT CGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTTCGA GGAGCCCACT TCTGCCTGAA GGTCTTCAGA 
GCTGAGGACT TACCACAGAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGTA ACAAGAAGAA CTTGGTGGAT CCCTTCATGG AAGTCAGCTT TGCAGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACC 
CTGCCTGCCA TGTTTCCCTC TATGTGTGAA AAAATGAGGA TTCGCGTCAT AGACTGGGAC 
CGCCTCACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCATGGAC 
CTGGACGACC ATCTGGGCTT CCTCCCCACC TTTGGGCCCT GCTATGTCAA CCTCTATGGC 
AGCCCCAGGG AGTTCACTGG CTTCCCGGAC CCCTACGCAG AACTGAACAT GGGCAAGGGG 
GAAGGTGTGG CCTACCGAGG TCGGGTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGAGCAGA AGGTGGAGGA CCTCCCTGCG GATGACATCC TTCGGGTGGA GAAGTACCTC 
CGGAGGCGCA AGTATTCCCT GTTTGCTGCC TTTTACTCGG CCTCCATGCT CCAGGATGTT 
GATGATGCCA TCCAGTTTGA GGTCAGCATC GGGAACTACG GTAACAAGTT CGACACGACC 
TGCCTGCCGC TGGCCTCCAC CACCCAGTAC AGCCGGGCGG TCTTTGACGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA CGTGAAGCCC GTGGTGGTGC TGTCATCTTA CTGGGAGGAC 
ATCAGCCACC GGGTCGAGGC TCAGAACCAG CTGCTCAGGA TTGCTGACCG GCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCCCTGAAG GCTCAGTGCA GCCCCGAGGA CGTGGACTCC 
CTGTTGGCTC AGCTGATGGA TGAGCTCATC TCCGACTGCA GCCAGCCCCT AGGTGACGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CAGTACCTGT ACAAGCTGCG TACCCGTCAC 
CTAGGCCAGA TCACTGAGGC GGCCCTGGCA CTGAAACTTG GCCGCAGCGA GCTCCCTGCT 
GCCCTGGAGC AGGCGGAAGA CTGGCTGCTG CGTGTCCGGG CCCTGGCAGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAG ACAAGCGAGT GGCGTACCAG 
CGGGTGCCCG CCCACGAGAT CCTCTTCTCC CGGCGGGGTC CCAACTACTG TGGCAAGAAT 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCAATGG AGGGCGTTCC TGGAGCCCGG 
ATGCCAGTGC AGATACGGGT GAAGCTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CGTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ACGGGAAAGA TTAAGCTACC CAAAGATAGC TTCCGCCCCT CGGCCGGCTG GACCTGGGCT 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTGCTCCATG ACATTGACGC GGGACACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACGGA TGTGAATGGG GAGAAGGTGC TTCCCAAGGA TGACATTGAG 
TGCCCCCTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTT 
GATGAGCAAG GCTGGGAGTA CAGTATCCCC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA CTACACGCAC CGTCGACGGC GCTGGGTCCG CCTGCGCAGG 
AGGGACCTCA GCCAGATGGA GGCACTGAAG AGGCACAGGC AGGCAGAGGC GGAGGGCGAG 
GGCTGGGAGT ACGCCTCGCT CTTTGGCTGG AAGTTCCACC TGGAGTACCG CAAGACGGAT 
GCCTTCCGCC GTCGCCGCTG GCGTCGCCGC ATGGAGCCCC TGGAGAAGAC AGGGCCTGCG 
GCCGTGTTTG CCCTCGAGGG GGCTCTGGGC GGCGTGGTGG ACGACAAGAG TGAAGATTCC 
ATGTCCGTGT CCACCTTGAG CTTTGGTGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CCCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACCGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACACTCAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGCATC GCCGAGCAAC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ATGACACCTA CGGTGCCGAC GAGTTCATGG GACGCTGCAT CTGTCAGCCA 
AGTCTGGAAC GGATGCCCCG GCTGGCCTGG TTCCCGTTGA TTAGGGGCAG CCAGCCAGCG 
GGTGAGCTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAGA AGCCGGCCAT CCACCATATT 
CCTGGTTTTG AGGTGCAGGA TACATCAGGA ATCCTGGAAG AGTCGGAGGA CACGGACCTG 
CCGTACCCAC CGCCTCAGAG GGAGGCCAAC ATCTACATGG TTCCCCAGAA CATCAAGCCA 
GTGCTCCAGC GTACCGCCAT AGAGATCCTG GCATGGGGCC TGCGGAACAT GAAGAGTTAC 
CAGCTGGCCA GTATCTCCTC TCCCAGCCTT GTGGTGGAGT GTGGAGGCCA GACCGTGCAG 
TCCTGTGTCA TCAAGAACCT CCGGAAGAAC CCCAACTTTG ACATCTGTAC CCTCTTCATG 
GAAGTGATGC TGCCCAGGGA GGAGCTCTAC TGTCCCCCCA TCGTGATCAA GGTCATTGAT 
AACCGCCAGT TTGGCCGCCG GCCTGTGGTG GGCCAGTGTA CCGTCCGCTC CCTGGAGAGC 
TTCATGTGTG ACCAGTACTC TGAAGAGAGT CCGTCCCCGC AGGGCGGCCC AGATGACGTG 
AGTTTACTCA GTCCTGGGGA AGACGTGCTC ATTGACATTG ATGATAAGGA GCCCCTCATC 
CCCATCCAGG AGGAGGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAAAAGT GTGGCCAGTA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ATGTGGAAGC CTTCGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACCGAA GACCCATCTG TTATTGGGGA GTTCAAGGGC 
CTCTTCAAAA TCTATCCCCT CCCAGAGGAC CCTGCCATTC CCATGCCCCC AAGACAGTTC 
CACCAACTGG CTGCCCAGGG GCCTCAGGAG TGCCTGGTCC GCATCTACAT CATCCGAGCA 
TTCGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCTT GTACTCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACTCTC 
TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT CGACCTGGAG 
AATAGGCTGC TGTCCAAGTT TGGAGCTCGC TGTGGACTCC CACAGACCTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAAGCTCC TGTGTACCAC ACAGACCGAG TGGTGTTTGA GGATAAAGAA 
TACACCATTG AGGAAATAGA GGCTGGCAAG GTCCTGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGTATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC 
GACCTATTTC CAAAGGCCCT GGGGAGGCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG CTGTATTATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CGGGGGAGAA GATGAGCGAT ATTTACGTGA AAGGTTGGAT GGTTGGCTTT 
GAAGAACACA AGCAAAAGAC AGATGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCTG AGCAAGTCTG CACTGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTCATA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTAGGCTC CTTGCAGCTG 
GACCTCAACC ACATGCCCAA ACCTGCCAAG ACAGCTGAGA AATGCTCCTT GGACCAGCTG 
GATGGCACCT TCCACCCAGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC TGTGAAGGGC 
TGGTGGCCCT GTGTGGCAGA AGAGGGCGAG AAGAAGATCC TGGCGGGCAA ACTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAACCCAACA TGAACCCTAA GCTTGAGGAC CCAAGGCGCC CTGATACCTC CTTCCTCTGG 
TTCACCTCTC CGTACAAGAC TATGAAGTTC ATCCTATGGC GGCGCTTCCG GTGTGCCATT 
ATTCTCTTCA TCATTCTCTT CATCCTACTG CTGTTCCTGG GCATCTTCGT CTATGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	101099290
Full Name	dysferlin
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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