MED12L cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED12L
Entrez Gene ID	101096834
Full Name	mediator complex subunit 12 like
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019810559.1	XP_019666118.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
XM_019810565.1	XP_019666124.1	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 12-like protein
XM_019810561.1	XP_019666120.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
XM_019810562.1	XP_019666121.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
XM_019810563.1	XP_019666122.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
XM_019810560.1	XP_019666119.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
XM_019810564.1	XP_019666123.1	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6

Pathways from BioSystems
Gene Ontology

The following MED12L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb48835	XM_019810559.1 Latest version!	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb48836	XM_019810560.1 Latest version!	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb48837	XM_019810561.1 Latest version!	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb48838	XM_019810562.1 Latest version!	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb48839	XM_019810563.1 Latest version!	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb48840	XM_019810564.1 Latest version!	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb48841	XM_019810565.1 Latest version!	Felis catus mediator complex subunit 12 like (MED12L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED12L ( XM_019810559.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019666118.1 Felis catus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

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CloneID	OFb48835
Clone ID Related Accession (Same CDS sequence)	XM_019810559.1
Accession Version	XM_019810559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGGA ACAGACTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACT ACTAATATTT TTGATGAAGT AGACAAGAAT 
GACTTTAAAA CTGACTTTGG TTCGGAGTTT CCAATTTTTT CTCCCATGCC TGGAGAATCC 
TGTGAGAATG CCAACCCTTC CCTGGGCAGA AGAATGTCGG TTAATGGTGA GAAGTTGCTT 
AAGAGAGAAA AACCCAGGGA GTTGATTTTT CCATCTAATT ATGACCTTCT GCGGCACCTG 
CAGTATGCAA CACATTTCCC CATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TGCTTTATGG AGTGGGCAAA GAGCGTGACG AAGCGAAGCA TCAGCTGAAG 
AAGATTACCA AAGATATTTT GAAAATCCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGAGATGAAG GACAAAAAAC CAGGAAGAAC AAACAGGAAG CATTTCCAAC TCTGGAGACT 
GTGTTCACAA AACTTCAGCT CCTTTCATAT TTTGATCAGC ATCAAGTGAC ATCTCAGATC 
TCCAATAATG TGCTGGAACA AATCACAAGC TTCGCTTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ATATTCAACT CATCTTTGAT CTTATGGAGC CAGCGCTGAA CATCAATGGA 
CTAATTGACT TCGCAATACA GCTGCTAAAT GAACTGAGTG TTGTGGAAGC CGAACTGCTC 
CTGAAATCCT CCAGCCTGGC AGGAAGTTAC ACGACGGGAC TGTGTGTGTG CATTGTGGCT 
GTTCTCAGGC GGTACCATAG TTGTCTGATC CTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT AAAGCATGTT GTGAACCCCT CAGAATGTTC TTCCCCCGAG 
AGATGCATCT TAGCCTACCT CTACGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTTAAGC AAACCATATA TAATAATGTG 
ATGCCTGCAA ATTCTAACTT GAGATGGGAT CCGGACTTCA TGATGGATTT TATTGAGAAT 
CCTTCAGCTC GTAGCATCAA TTACTCAATG CTGGGCAAGA TCCTTAGTGA CAATGCAGCC 
AATCGCTACA GCTTCGTCTG TAACACGCTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGAAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTGA CAGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGAGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA TGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTCTCCC TGGAGGACGT CGTGCAGCAC 
GTCGCACTTC CCTCTCTCCT AGCAGCAGCT TGTGGAGATG CAGATGCTGA GCCGGGGGCG 
AGAATGACAT GCCGACTCCT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTGTTACCT 
CAAGCAACCG GTGAGCCGAC AGCTATAAAA TCCCCAACTG CATGTTCATT TGGAGGCAAA 
CCTTTCCCTG GAATAAGATC ATCTTGTGAT AGACACCTCT TAGCCGCCGC TCACAATAGC 
ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA AAAGCAATTA TGATGCTTGG AGATGCCAAA 
ATTGGCAATA ACAATGTCAG CTCTTTAAAG AATGATGACT TCACCATGAG AGGTTTACGG 
CGTGATGGGA ATGCTGATGA TATCTGGACT GCTTCACAAA ATTCAAAATC CTGTGGGAAA 
AGCATTTCCA TAGAAACTGC CAATTTAAGA GAATATGCTA GATATGTACT GAGGACTATC 
TGTCAACAGG AATGGGTAGG AGAACATTGC CTAAAAGAAC CTGAAAGGTT GTGCACAGAC 
AAAGAGCTTA TATTGGACCC TGTGCTTTCA AATATGCAAG CACAGAAATT ATTGCAGCTT 
ATTTGTTATC CTCATGGTAT TAAAGAATGT ACGGAGGGCG ACAATCTGCA GAGACAGCAC 
ATTAAGCGCA TTCTTCAGAA TCTTGAGCAG TGGACACTGA GGCAGTCCTG GCTAGAACTT 
CAGTTAATGA TCAAACAGTG CTTGAAGGAC CCTGGCTCTG GTTCAGTGGC TGAGATGAAC 
AACTTACTGG ACAATATTGC AAAGGCAACA ATAGAGGTAT TCCAGCAGTC TGCTGACTTG 
AACAATAATT CTTCTAATTC TGGAATGGGC CTCTTCAACC CAAATGGGAT TGCAACTACT 
GATGCTGGTA GCACAAGGCA GAATGGAATA AAGACATTTC TAAGTTCCTC TGAACGCAGG 
GGTGTATGGC TGGTGGCCCC CCTAATTGCC AGGCTGCCGA CCTCTGTGCA AGGAAGAGTA 
TTGAAAGCTG CAGGAGAGGA ACTGGAGAAG GGACAGCACT TGGGCTCTTC TTCAAAAAAG 
GAAAGAGACA GACAAAAACA GAAAAGTATG TCTCTTTTGA GTCAACAACC TTTCCTCTCA 
CTGGTCCTTA CCTGCCTTAA GGGACAAGAT GAACAACGGG AAGGCCTCCT AACATCTCTT 
CAGAATCAGG TTAATCAGAT TTTAAGTAAT TGGAGAGAAG AACGATACCA GGATGACATA 
AAAGCACGGC AGATGATGCA TGAAGCATTG CAACTTCGCC TAAATTTGGT AGGAGGAATG 
TTTGACACGG TGCAGAGGAG CACCCAGTGG ACAACAGACT GGGCCCTCCT CCTGCTTCAG 
ATCATAACTT CTGGAACTGT TGACATGCAC ACTAACAATG AATTATTCAC AACAGTTCTT 
GACATGCTGG GTGTGTTAAT CAATGGAACA TTAGCCTCAG ACCTATCAAA TGCATCCCCA 
GGGGGATCTG AAGAGAACAA ACGTGCATAC ATGAATTTAG TAAAGAAACT GAAGAAAGAG 
CTAGGAGACA AGAGATCAGA AAGTATTGAC AAAGTTCGAC AGTTGCTACC TTTGCCAAAA 
CAGACATGTG ATGTCATTAC TTGTGAACCT ATGGGTTCCT TGATTGACAC AAAGGGAAAC 
AAAATTGCTG GATTCGACTC CATAGATAAA AAACAGGGTC TTCAGGTGTC TACAAAGCAG 
AAGGTGTCCC CATGGGATTT GTTTGAGGGT CAGAAGAACC CAGCTCCTCT GTCTTGGGCC 
TGGTTCGGGA CTGTCCGAGT GGACCGAAAG GTGATCAAGT ACGAGGAGCA GCATCACCTG 
CTGTTGTATC ACACACACCC CATGCCCAAG CCCCGAAGTT ACTACCTCGA ACCGCTGCCC 
CTGCCTCCCG AGGAGGAAGA GGAGGAGCCC ACATCTCCCA TCTCTCAGGA ACCAGAGCGG 
AAATCAGCTG AGCTGTCAGA TCAGGGAAAA ACCGCAACAG ATGAAGAGAA GAAAACAAAA 
GGAAGGAAGC GCAAGACGAA ATCAAGCTCA AGAGTTGACG AGTATCCACA GAGCAACATA 
TACCGAGTGC CTCCTAACTA CTCACCCATT TCCTCCCAGA TGATGCACCA TCCGCAGTCC 
ACCTTATGGG GCTATAACCT CATGGGTCAG CCCCAGCAGC CGGGTTTTTT CCTTCAGAAC 
CAATCTCTTA CTCCAGGTGG TTCTAGATTG GACCCCACGG GCTCCTTCGT CCCAACGAAT 
ACCAAACAAG CTCTGTCCAA CATGCTGCAG CGGCGCTCGG GGGCCATGAT GCAGCCGCCG 
CCCCTCCACG CCATCACATC GCAGCAGCAG CTGATACAGA TGAAGCTTCT GCAGCAGCAG 
CAGCAGCAGC GGCTTCTCCG GCAAGCCCAG GCTCGACCTT TCCAGCAGGA CTTCTGTAAC 
TCTGCTGTCT CCCTTCTGGA AGACTTTGAC AACATAATGG GCCCGCCAGG GGACCAGGCT 
GCTCTCTTTG CTGCACAAGC ACGGCCCTCC CCTCAGCTCC CCCAGTATCC AGGGCTGCAG 
CAAGCACAGA CCATGCCCCA GGGCTATACG ATGTATGGAA CGCAGATGCC TTTGCAGCAG 
ACCCCTCAGC AGCAGGCTGG TGGCGTAGTC CTGTCTCCTA GCTATAACTC CAGGACCTAT 
CCGGCCACAC ATTCCAACCC GGCGCTAATG GAAAGACTCA GACAGATGCA GCAGCAGCCC 
AGTGGCTATG TTCAGCAGCA GGCGTCACCG TACCTGCAGC CCTTGACCAG CTCTCAGAGA 
CTAAATCATC AGTCGCTTCA GCAGAGCCCT CTGGTAGGTG GAGGAATCGA TGCAGTGCTC 
ACTCCCACGC ATCCAAGCCT CCCCTCGGTG CCCCTGTCTC AGGACCCAAT GAGAACCAGA 
CAGCCGCAGG TTCGACAGCA GCAGAGACTC CTCCAGATGC AGCAGCCCCC CCAGCCCCAG 
CAGCCCCCGC AGCCCCAGAG TCAGACCCTT GGTCTCCAAG CGATGCAGCC CCAGCAGCCT 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AACAGACGGC CGCCCTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCTAGTAAC CAGCCACAGC AAGGAGTGAC TCCCTATGGG 
CATCCATCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666118.1
CDS	204..6821
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIAAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQATDPNLEWTQISTRYLREQLAKISDFYHMASSTSDGPVPVPPDVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHMMIGPNNSSIGAPSPGPPGPVMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVSASTRPRSPAGENVDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEAKHQLKKITKDILKILNKKSTTETGVGDEGQKTRKNKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGEPTAIKSPTACSFGGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNNVSSLKNDDFTMRGLRRDGNADDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNSGMGLFNPNGIATTDAGSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPMPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLMGQPQQPGFFLQNQSLTPGGSRLDPTGSFVPTNTKQALSNMLQRRSGAMMQPPPLHAITSQQQLIQMKLLQQQQQQRLLRQAQARPFQQDFCNSAVSLLEDFDNIMGPPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGGVVLSPSYNSRTYPATHSNPALMERLRQMQQQPSGYVQQQASPYLQPLTSSQRLNHQSLQQSPLVGGGIDAVLTPTHPSLPSVPLSQDPMRTRQPQVRQQQRLLQMQQPPQPQQPPQPQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_019810559.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810559.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGGA ACAGACTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACT ACTAATATTT TTGATGAAGT AGACAAGAAT 
GACTTTAAAA CTGACTTTGG TTCGGAGTTT CCAATTTTTT CTCCCATGCC TGGAGAATCC 
TGTGAGAATG CCAACCCTTC CCTGGGCAGA AGAATGTCGG TTAATGGTGA GAAGTTGCTT 
AAGAGAGAAA AACCCAGGGA GTTGATTTTT CCATCTAATT ATGACCTTCT GCGGCACCTG 
CAGTATGCAA CACATTTCCC CATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TGCTTTATGG AGTGGGCAAA GAGCGTGACG AAGCGAAGCA TCAGCTGAAG 
AAGATTACCA AAGATATTTT GAAAATCCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGAGATGAAG GACAAAAAAC CAGGAAGAAC AAACAGGAAG CATTTCCAAC TCTGGAGACT 
GTGTTCACAA AACTTCAGCT CCTTTCATAT TTTGATCAGC ATCAAGTGAC ATCTCAGATC 
TCCAATAATG TGCTGGAACA AATCACAAGC TTCGCTTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ATATTCAACT CATCTTTGAT CTTATGGAGC CAGCGCTGAA CATCAATGGA 
CTAATTGACT TCGCAATACA GCTGCTAAAT GAACTGAGTG TTGTGGAAGC CGAACTGCTC 
CTGAAATCCT CCAGCCTGGC AGGAAGTTAC ACGACGGGAC TGTGTGTGTG CATTGTGGCT 
GTTCTCAGGC GGTACCATAG TTGTCTGATC CTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT AAAGCATGTT GTGAACCCCT CAGAATGTTC TTCCCCCGAG 
AGATGCATCT TAGCCTACCT CTACGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTTAAGC AAACCATATA TAATAATGTG 
ATGCCTGCAA ATTCTAACTT GAGATGGGAT CCGGACTTCA TGATGGATTT TATTGAGAAT 
CCTTCAGCTC GTAGCATCAA TTACTCAATG CTGGGCAAGA TCCTTAGTGA CAATGCAGCC 
AATCGCTACA GCTTCGTCTG TAACACGCTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGAAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTGA CAGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGAGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA TGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTCTCCC TGGAGGACGT CGTGCAGCAC 
GTCGCACTTC CCTCTCTCCT AGCAGCAGCT TGTGGAGATG CAGATGCTGA GCCGGGGGCG 
AGAATGACAT GCCGACTCCT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTGTTACCT 
CAAGCAACCG GTGAGCCGAC AGCTATAAAA TCCCCAACTG CATGTTCATT TGGAGGCAAA 
CCTTTCCCTG GAATAAGATC ATCTTGTGAT AGACACCTCT TAGCCGCCGC TCACAATAGC 
ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA AAAGCAATTA TGATGCTTGG AGATGCCAAA 
ATTGGCAATA ACAATGTCAG CTCTTTAAAG AATGATGACT TCACCATGAG AGGTTTACGG 
CGTGATGGGA ATGCTGATGA TATCTGGACT GCTTCACAAA ATTCAAAATC CTGTGGGAAA 
AGCATTTCCA TAGAAACTGC CAATTTAAGA GAATATGCTA GATATGTACT GAGGACTATC 
TGTCAACAGG AATGGGTAGG AGAACATTGC CTAAAAGAAC CTGAAAGGTT GTGCACAGAC 
AAAGAGCTTA TATTGGACCC TGTGCTTTCA AATATGCAAG CACAGAAATT ATTGCAGCTT 
ATTTGTTATC CTCATGGTAT TAAAGAATGT ACGGAGGGCG ACAATCTGCA GAGACAGCAC 
ATTAAGCGCA TTCTTCAGAA TCTTGAGCAG TGGACACTGA GGCAGTCCTG GCTAGAACTT 
CAGTTAATGA TCAAACAGTG CTTGAAGGAC CCTGGCTCTG GTTCAGTGGC TGAGATGAAC 
AACTTACTGG ACAATATTGC AAAGGCAACA ATAGAGGTAT TCCAGCAGTC TGCTGACTTG 
AACAATAATT CTTCTAATTC TGGAATGGGC CTCTTCAACC CAAATGGGAT TGCAACTACT 
GATGCTGGTA GCACAAGGCA GAATGGAATA AAGACATTTC TAAGTTCCTC TGAACGCAGG 
GGTGTATGGC TGGTGGCCCC CCTAATTGCC AGGCTGCCGA CCTCTGTGCA AGGAAGAGTA 
TTGAAAGCTG CAGGAGAGGA ACTGGAGAAG GGACAGCACT TGGGCTCTTC TTCAAAAAAG 
GAAAGAGACA GACAAAAACA GAAAAGTATG TCTCTTTTGA GTCAACAACC TTTCCTCTCA 
CTGGTCCTTA CCTGCCTTAA GGGACAAGAT GAACAACGGG AAGGCCTCCT AACATCTCTT 
CAGAATCAGG TTAATCAGAT TTTAAGTAAT TGGAGAGAAG AACGATACCA GGATGACATA 
AAAGCACGGC AGATGATGCA TGAAGCATTG CAACTTCGCC TAAATTTGGT AGGAGGAATG 
TTTGACACGG TGCAGAGGAG CACCCAGTGG ACAACAGACT GGGCCCTCCT CCTGCTTCAG 
ATCATAACTT CTGGAACTGT TGACATGCAC ACTAACAATG AATTATTCAC AACAGTTCTT 
GACATGCTGG GTGTGTTAAT CAATGGAACA TTAGCCTCAG ACCTATCAAA TGCATCCCCA 
GGGGGATCTG AAGAGAACAA ACGTGCATAC ATGAATTTAG TAAAGAAACT GAAGAAAGAG 
CTAGGAGACA AGAGATCAGA AAGTATTGAC AAAGTTCGAC AGTTGCTACC TTTGCCAAAA 
CAGACATGTG ATGTCATTAC TTGTGAACCT ATGGGTTCCT TGATTGACAC AAAGGGAAAC 
AAAATTGCTG GATTCGACTC CATAGATAAA AAACAGGGTC TTCAGGTGTC TACAAAGCAG 
AAGGTGTCCC CATGGGATTT GTTTGAGGGT CAGAAGAACC CAGCTCCTCT GTCTTGGGCC 
TGGTTCGGGA CTGTCCGAGT GGACCGAAAG GTGATCAAGT ACGAGGAGCA GCATCACCTG 
CTGTTGTATC ACACACACCC CATGCCCAAG CCCCGAAGTT ACTACCTCGA ACCGCTGCCC 
CTGCCTCCCG AGGAGGAAGA GGAGGAGCCC ACATCTCCCA TCTCTCAGGA ACCAGAGCGG 
AAATCAGCTG AGCTGTCAGA TCAGGGAAAA ACCGCAACAG ATGAAGAGAA GAAAACAAAA 
GGAAGGAAGC GCAAGACGAA ATCAAGCTCA AGAGTTGACG AGTATCCACA GAGCAACATA 
TACCGAGTGC CTCCTAACTA CTCACCCATT TCCTCCCAGA TGATGCACCA TCCGCAGTCC 
ACCTTATGGG GCTATAACCT CATGGGTCAG CCCCAGCAGC CGGGTTTTTT CCTTCAGAAC 
CAATCTCTTA CTCCAGGTGG TTCTAGATTG GACCCCACGG GCTCCTTCGT CCCAACGAAT 
ACCAAACAAG CTCTGTCCAA CATGCTGCAG CGGCGCTCGG GGGCCATGAT GCAGCCGCCG 
CCCCTCCACG CCATCACATC GCAGCAGCAG CTGATACAGA TGAAGCTTCT GCAGCAGCAG 
CAGCAGCAGC GGCTTCTCCG GCAAGCCCAG GCTCGACCTT TCCAGCAGGA CTTCTGTAAC 
TCTGCTGTCT CCCTTCTGGA AGACTTTGAC AACATAATGG GCCCGCCAGG GGACCAGGCT 
GCTCTCTTTG CTGCACAAGC ACGGCCCTCC CCTCAGCTCC CCCAGTATCC AGGGCTGCAG 
CAAGCACAGA CCATGCCCCA GGGCTATACG ATGTATGGAA CGCAGATGCC TTTGCAGCAG 
ACCCCTCAGC AGCAGGCTGG TGGCGTAGTC CTGTCTCCTA GCTATAACTC CAGGACCTAT 
CCGGCCACAC ATTCCAACCC GGCGCTAATG GAAAGACTCA GACAGATGCA GCAGCAGCCC 
AGTGGCTATG TTCAGCAGCA GGCGTCACCG TACCTGCAGC CCTTGACCAG CTCTCAGAGA 
CTAAATCATC AGTCGCTTCA GCAGAGCCCT CTGGTAGGTG GAGGAATCGA TGCAGTGCTC 
ACTCCCACGC ATCCAAGCCT CCCCTCGGTG CCCCTGTCTC AGGACCCAAT GAGAACCAGA 
CAGCCGCAGG TTCGACAGCA GCAGAGACTC CTCCAGATGC AGCAGCCCCC CCAGCCCCAG 
CAGCCCCCGC AGCCCCAGAG TCAGACCCTT GGTCTCCAAG CGATGCAGCC CCAGCAGCCT 
TTGTTTCCCA GGCAAGGCTT GCAGCAGACC CAGCAGCAGC AACAGACGGC CGCCCTGGTG 
CGGCAGCTCC AGAAGCAGCT TTCTAGTAAC CAGCCACAGC AAGGAGTGAC TCCCTATGGG 
CATCCATCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_019810560.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019666119.1 Felis catus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

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CloneID	OFb48836
Clone ID Related Accession (Same CDS sequence)	XM_019810560.1
Accession Version	XM_019810560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTTCCTCAGG TTCCAATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCTAAATAT 
TCAGTGCCAA TGGTTCGAGC CACGTGGCTG ATCAAGATGA CGTGTGCCTA TTATTCTGCT 
ATTTCTGAAG CTAAAATTAA GAAACGTCAG GCTACTGATC CTAATTTGGA GTGGACACAA 
ATATCTACCA GATATCTTCG AGAGCAGCTG GCCAAGATTT CTGACTTTTA CCACATGGCT 
TCCAGCACAA GTGATGGTCC TGTCCCAGTG CCACCAGATG TGGAGCAAGC CATGAAGCAA 
TGGGAATACA ACGAAAAGTT GGCATTTCAC ATGTTCCAGG AAGGAATGCT AGAAAAACAT 
GAATATTTGA CATGGATCTT GGATGTGTTA GAGAAGATCA GACCGATGGA TGATGACCTT 
CTTAAACTCT TGTTACCACT CATGCTGCAG TATTCGGACG AGTTTGTTCA GTCGGCTTAC 
CTGTCTCGTC GTCTTGCCTA CTTTTGTGCC CGGCGTCTTG CCTTGCTGCT GAGCGATAGC 
CCCAGCCTCC TTGCTGCCCA CTCGCCCCAC ATGATGATAG GACCAAACAA CTCAAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GTCATGAGTC CCGTGCAGCT GGCCTTCTCA 
GACTTCCTCT CCTGTGCACA GCATGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTCACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ATTCCACAAA CGACAATAAG 
AGCGCGAACC CAGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG TCTCCCCATG 
CCGGGTGGGA ACACAGCTTT CAATCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCAACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAAGTGTT GGATCGACAT 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGATAA CCAAGAGGTC GCCCCCAACG ATGAAGCTGT GGTGACCTTG 
CTGTGTGAAT GGGCGGTGAG CTGCAAACGG TCAGGCAAGC ACAGGGCCAT GGCTGTGGCA 
AAACTCTTGG AGAAGAGGCA GGCGGAAATC GAAGCAGAGA GATGTGGTGA ATCAGAGGTC 
TTGGATGAGA AGGAGTCCAT TTCTTCAGCC TCTCTTGCTG GATCTAGTTT GCCTGTTTTC 
CAGAATGTGC TACTAAGGTT TTTAGATACA CAGGCCCCCT CATTATCGGA TCCAAACAGT 
GAATGTGAAA AAGTAGAATT TGTGAATTTG GTGCTGCTCT TCTGTGAGTT CATCCGACAC 
GATGTCTTCT CCCATGATGC GTACATGTGT ACCCTCATAT CTCGGGGAGA CTTGTCAGTC 
AGCGCCTCGA CTCGGCCGCG ATCGCCAGCA GGAGAAAACG TAGATGAGCA CTATCCAAAG 
GACCATGACA TGAAAATGGA GGAACAGACT ATTATGGCGC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAG 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAA TCCTGTGAGA ATGCCAACCC TTCCCTGGGC 
AGAAGAATGT CGGTTAATGG TGAGAAGTTG CTTAAGAGAG AAAAACCCAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT TCTGCGGCAC CTGCAGTATG CAACACATTT CCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTGCTTTA TGGAGTGGGC 
AAAGAGCGTG ACGAAGCGAA GCATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCACCAC AGAGACAGGG GTTGGAGATG AAGGACAAAA AACCAGGAAG 
AACAAACAGG AAGCATTTCC AACTCTGGAG ACTGTGTTCA CAAAACTTCA GCTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATCTCCAATA ATGTGCTGGA ACAAATCACA 
AGCTTCGCTT CAGGAACATC CTATCATCTC CCTTTGGCTC ACCATATTCA ACTCATCTTT 
GATCTTATGG AGCCAGCGCT GAACATCAAT GGACTAATTG ACTTCGCAAT ACAGCTGCTA 
AATGAACTGA GTGTTGTGGA AGCCGAACTG CTCCTGAAAT CCTCCAGCCT GGCAGGAAGT 
TACACGACGG GACTGTGTGT GTGCATTGTG GCTGTTCTCA GGCGGTACCA TAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGGT TGTGTGGTGT GGTAAAGCAT 
GTTGTGAACC CCTCAGAATG TTCTTCCCCC GAGAGATGCA TCTTAGCCTA CCTCTACGAT 
CTCTATGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTTA AGCAAACCAT ATATAATAAT GTGATGCCTG CAAATTCTAA CTTGAGATGG 
GATCCGGACT TCATGATGGA TTTTATTGAG AATCCTTCAG CTCGTAGCAT CAATTACTCA 
ATGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGTAACACG 
CTCATGAATG TATGTATGGG CCATCAGGAT GCTGGAAGGA TTAACGACAT AGCCAATTTC 
TCCTCTGAGC TGACAGCTTG CTGCACTGTT CTTAGTTCAG AATGGCTGGG AGTTCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGGTTTAATG ATGTACTTTG CACTGTAGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAG CACGTCGCAC TTCCCTCTCT CCTAGCAGCA 
GCTTGTGGAG ATGCAGATGC TGAGCCGGGG GCGAGAATGA CATGCCGACT CCTGCTTCAT 
CTCTTCCGAG CTCCCCAGGC CTGCTTGTTA CCTCAAGCAA CCGGCAAACC TTTCCCTGGA 
ATAAGATCAT CTTGTGATAG ACACCTCTTA GCCGCCGCTC ACAATAGCAT TGAAGTGGGA 
GCCGTGTTTG CTGTCTTAAA AGCAATTATG ATGCTTGGAG ATGCCAAAAT TGGCAATAAC 
AATGTCAGCT CTTTAAAGAA TGATGACTTC ACCATGAGAG GTTTACGGCG TGATGGGAAT 
GCTGATGATA TCTGGACTGC TTCACAAAAT TCAAAATCCT GTGGGAAAAG CATTTCCATA 
GAAACTGCCA ATTTAAGAGA ATATGCTAGA TATGTACTGA GGACTATCTG TCAACAGGAA 
TGGGTAGGAG AACATTGCCT AAAAGAACCT GAAAGGTTGT GCACAGACAA AGAGCTTATA 
TTGGACCCTG TGCTTTCAAA TATGCAAGCA CAGAAATTAT TGCAGCTTAT TTGTTATCCT 
CATGGTATTA AAGAATGTAC GGAGGGCGAC AATCTGCAGA GACAGCACAT TAAGCGCATT 
CTTCAGAATC TTGAGCAGTG GACACTGAGG CAGTCCTGGC TAGAACTTCA GTTAATGATC 
AAACAGTGCT TGAAGGACCC TGGCTCTGGT TCAGTGGCTG AGATGAACAA CTTACTGGAC 
AATATTGCAA AGGCAACAAT AGAGGTATTC CAGCAGTCTG CTGACTTGAA CAATAATTCT 
TCTAATTCTG GAATGGGCCT CTTCAACCCA AATGGGATTG CAACTACTGA TGCTGGTAGC 
ACAAGGCAGA ATGGAATAAA GACATTTCTA AGTTCCTCTG AACGCAGGGG TGTATGGCTG 
GTGGCCCCCC TAATTGCCAG GCTGCCGACC TCTGTGCAAG GAAGAGTATT GAAAGCTGCA 
GGAGAGGAAC TGGAGAAGGG ACAGCACTTG GGCTCTTCTT CAAAAAAGGA AAGAGACAGA 
CAAAAACAGA AAAGTATGTC TCTTTTGAGT CAACAACCTT TCCTCTCACT GGTCCTTACC 
TGCCTTAAGG GACAAGATGA ACAACGGGAA GGCCTCCTAA CATCTCTTCA GAATCAGGTT 
AATCAGATTT TAAGTAATTG GAGAGAAGAA CGATACCAGG ATGACATAAA AGCACGGCAG 
ATGATGCATG AAGCATTGCA ACTTCGCCTA AATTTGGTAG GAGGAATGTT TGACACGGTG 
CAGAGGAGCA CCCAGTGGAC AACAGACTGG GCCCTCCTCC TGCTTCAGAT CATAACTTCT 
GGAACTGTTG ACATGCACAC TAACAATGAA TTATTCACAA CAGTTCTTGA CATGCTGGGT 
GTGTTAATCA ATGGAACATT AGCCTCAGAC CTATCAAATG CATCCCCAGG GGGATCTGAA 
GAGAACAAAC GTGCATACAT GAATTTAGTA AAGAAACTGA AGAAAGAGCT AGGAGACAAG 
AGATCAGAAA GTATTGACAA AGTTCGACAG TTGCTACCTT TGCCAAAACA GACATGTGAT 
GTCATTACTT GTGAACCTAT GGGTTCCTTG ATTGACACAA AGGGAAACAA AATTGCTGGA 
TTCGACTCCA TAGATAAAAA ACAGGGTCTT CAGGTGTCTA CAAAGCAGAA GGTGTCCCCA 
TGGGATTTGT TTGAGGGTCA GAAGAACCCA GCTCCTCTGT CTTGGGCCTG GTTCGGGACT 
GTCCGAGTGG ACCGAAAGGT GATCAAGTAC GAGGAGCAGC ATCACCTGCT GTTGTATCAC 
ACACACCCCA TGCCCAAGCC CCGAAGTTAC TACCTCGAAC CGCTGCCCCT GCCTCCCGAG 
GAGGAAGAGG AGGAGCCCAC ATCTCCCATC TCTCAGGAAC CAGAGCGGAA ATCAGCTGAG 
CTGTCAGATC AGGGAAAAAC CGCAACAGAT GAAGAGAAGA AAACAAAAGG AAGGAAGCGC 
AAGACGAAAT CAAGCTCAAG AGTTGACGAG TATCCACAGA GCAACATATA CCGAGTGCCT 
CCTAACTACT CACCCATTTC CTCCCAGATG ATGCACCATC CGCAGTCCAC CTTATGGGGC 
TATAACCTCA TGGGTCAGCC CCAGCAGCCG GGTTTTTTCC TTCAGAACCA ATCTCTTACT 
CCAGGTGGTT CTAGATTGGA CCCCACGGGC TCCTTCGTCC CAACGAATAC CAAACAAGCT 
CTGTCCAACA TGCTGCAGCG GCGCTCGGGG GCCATGATGC AGCCGCCGCC CCTCCACGCC 
ATCACATCGC AGCAGCAGCT GATACAGATG AAGCTTCTGC AGCAGCAGCA GCAGCAGCGG 
CTTCTCCGGC AAGCCCAGGC TCGACCTTTC CAGCAGGACT TCTGTAACTC TGCTGTCTCC 
CTTCTGGAAG ACTTTGACAA CATAATGGGC CCGCCAGGGG ACCAGGCTGC TCTCTTTGCT 
GCACAAGCAC GGCCCTCCCC TCAGCTCCCC CAGTATCCAG GGCTGCAGCA AGCACAGACC 
ATGCCCCAGG GCTATACGAT GTATGGAACG CAGATGCCTT TGCAGCAGAC CCCTCAGCAG 
CAGGCTGGTG GCGTAGTCCT GTCTCCTAGC TATAACTCCA GGACCTATCC GGCCACACAT 
TCCAACCCGG CGCTAATGGA AAGACTCAGA CAGATGCAGC AGCAGCCCAG TGGCTATGTT 
CAGCAGCAGG CGTCACCGTA CCTGCAGCCC TTGACCAGCT CTCAGAGACT AAATCATCAG 
TCGCTTCAGC AGAGCCCTCT GGTAGGTGGA GGAATCGATG CAGTGCTCAC TCCCACGCAT 
CCAAGCCTCC CCTCGGTGCC CCTGTCTCAG GACCCAATGA GAACCAGACA GCCGCAGGTT 
CGACAGCAGC AGAGACTCCT CCAGATGCAG CAGCCCCCCC AGCCCCAGCA GCCCCCGCAG 
CCCCAGAGTC AGACCCTTGG TCTCCAAGCG ATGCAGCCCC AGCAGCCTTT GTTTCCCAGG 
CAAGGCTTGC AGCAGACCCA GCAGCAGCAA CAGACGGCCG CCCTGGTGCG GCAGCTCCAG 
AAGCAGCTTT CTAGTAACCA GCCACAGCAA GGAGTGACTC CCTATGGGCA TCCATCACAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666119.1
CDS	204..6809
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIAAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQATDPNLEWTQISTRYLREQLAKISDFYHMASSTSDGPVPVPPDVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHMMIGPNNSSIGAPSPGPPGPVMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVSASTRPRSPAGENVDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEAKHQLKKITKDILKILNKKSTTETGVGDEGQKTRKNKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNNVSSLKNDDFTMRGLRRDGNADDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNSGMGLFNPNGIATTDAGSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPMPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLMGQPQQPGFFLQNQSLTPGGSRLDPTGSFVPTNTKQALSNMLQRRSGAMMQPPPLHAITSQQQLIQMKLLQQQQQQRLLRQAQARPFQQDFCNSAVSLLEDFDNIMGPPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGGVVLSPSYNSRTYPATHSNPALMERLRQMQQQPSGYVQQQASPYLQPLTSSQRLNHQSLQQSPLVGGGIDAVLTPTHPSLPSVPLSQDPMRTRQPQVRQQQRLLQMQQPPQPQQPPQPQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_019810560.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810560.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTTCCTCAGG TTCCAATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCTAAATAT 
TCAGTGCCAA TGGTTCGAGC CACGTGGCTG ATCAAGATGA CGTGTGCCTA TTATTCTGCT 
ATTTCTGAAG CTAAAATTAA GAAACGTCAG GCTACTGATC CTAATTTGGA GTGGACACAA 
ATATCTACCA GATATCTTCG AGAGCAGCTG GCCAAGATTT CTGACTTTTA CCACATGGCT 
TCCAGCACAA GTGATGGTCC TGTCCCAGTG CCACCAGATG TGGAGCAAGC CATGAAGCAA 
TGGGAATACA ACGAAAAGTT GGCATTTCAC ATGTTCCAGG AAGGAATGCT AGAAAAACAT 
GAATATTTGA CATGGATCTT GGATGTGTTA GAGAAGATCA GACCGATGGA TGATGACCTT 
CTTAAACTCT TGTTACCACT CATGCTGCAG TATTCGGACG AGTTTGTTCA GTCGGCTTAC 
CTGTCTCGTC GTCTTGCCTA CTTTTGTGCC CGGCGTCTTG CCTTGCTGCT GAGCGATAGC 
CCCAGCCTCC TTGCTGCCCA CTCGCCCCAC ATGATGATAG GACCAAACAA CTCAAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GTCATGAGTC CCGTGCAGCT GGCCTTCTCA 
GACTTCCTCT CCTGTGCACA GCATGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTCACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ATTCCACAAA CGACAATAAG 
AGCGCGAACC CAGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG TCTCCCCATG 
CCGGGTGGGA ACACAGCTTT CAATCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCAACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAAGTGTT GGATCGACAT 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGATAA CCAAGAGGTC GCCCCCAACG ATGAAGCTGT GGTGACCTTG 
CTGTGTGAAT GGGCGGTGAG CTGCAAACGG TCAGGCAAGC ACAGGGCCAT GGCTGTGGCA 
AAACTCTTGG AGAAGAGGCA GGCGGAAATC GAAGCAGAGA GATGTGGTGA ATCAGAGGTC 
TTGGATGAGA AGGAGTCCAT TTCTTCAGCC TCTCTTGCTG GATCTAGTTT GCCTGTTTTC 
CAGAATGTGC TACTAAGGTT TTTAGATACA CAGGCCCCCT CATTATCGGA TCCAAACAGT 
GAATGTGAAA AAGTAGAATT TGTGAATTTG GTGCTGCTCT TCTGTGAGTT CATCCGACAC 
GATGTCTTCT CCCATGATGC GTACATGTGT ACCCTCATAT CTCGGGGAGA CTTGTCAGTC 
AGCGCCTCGA CTCGGCCGCG ATCGCCAGCA GGAGAAAACG TAGATGAGCA CTATCCAAAG 
GACCATGACA TGAAAATGGA GGAACAGACT ATTATGGCGC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAG 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAA TCCTGTGAGA ATGCCAACCC TTCCCTGGGC 
AGAAGAATGT CGGTTAATGG TGAGAAGTTG CTTAAGAGAG AAAAACCCAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT TCTGCGGCAC CTGCAGTATG CAACACATTT CCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTGCTTTA TGGAGTGGGC 
AAAGAGCGTG ACGAAGCGAA GCATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCACCAC AGAGACAGGG GTTGGAGATG AAGGACAAAA AACCAGGAAG 
AACAAACAGG AAGCATTTCC AACTCTGGAG ACTGTGTTCA CAAAACTTCA GCTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATCTCCAATA ATGTGCTGGA ACAAATCACA 
AGCTTCGCTT CAGGAACATC CTATCATCTC CCTTTGGCTC ACCATATTCA ACTCATCTTT 
GATCTTATGG AGCCAGCGCT GAACATCAAT GGACTAATTG ACTTCGCAAT ACAGCTGCTA 
AATGAACTGA GTGTTGTGGA AGCCGAACTG CTCCTGAAAT CCTCCAGCCT GGCAGGAAGT 
TACACGACGG GACTGTGTGT GTGCATTGTG GCTGTTCTCA GGCGGTACCA TAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGGT TGTGTGGTGT GGTAAAGCAT 
GTTGTGAACC CCTCAGAATG TTCTTCCCCC GAGAGATGCA TCTTAGCCTA CCTCTACGAT 
CTCTATGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTTA AGCAAACCAT ATATAATAAT GTGATGCCTG CAAATTCTAA CTTGAGATGG 
GATCCGGACT TCATGATGGA TTTTATTGAG AATCCTTCAG CTCGTAGCAT CAATTACTCA 
ATGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGTAACACG 
CTCATGAATG TATGTATGGG CCATCAGGAT GCTGGAAGGA TTAACGACAT AGCCAATTTC 
TCCTCTGAGC TGACAGCTTG CTGCACTGTT CTTAGTTCAG AATGGCTGGG AGTTCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGGTTTAATG ATGTACTTTG CACTGTAGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAG CACGTCGCAC TTCCCTCTCT CCTAGCAGCA 
GCTTGTGGAG ATGCAGATGC TGAGCCGGGG GCGAGAATGA CATGCCGACT CCTGCTTCAT 
CTCTTCCGAG CTCCCCAGGC CTGCTTGTTA CCTCAAGCAA CCGGCAAACC TTTCCCTGGA 
ATAAGATCAT CTTGTGATAG ACACCTCTTA GCCGCCGCTC ACAATAGCAT TGAAGTGGGA 
GCCGTGTTTG CTGTCTTAAA AGCAATTATG ATGCTTGGAG ATGCCAAAAT TGGCAATAAC 
AATGTCAGCT CTTTAAAGAA TGATGACTTC ACCATGAGAG GTTTACGGCG TGATGGGAAT 
GCTGATGATA TCTGGACTGC TTCACAAAAT TCAAAATCCT GTGGGAAAAG CATTTCCATA 
GAAACTGCCA ATTTAAGAGA ATATGCTAGA TATGTACTGA GGACTATCTG TCAACAGGAA 
TGGGTAGGAG AACATTGCCT AAAAGAACCT GAAAGGTTGT GCACAGACAA AGAGCTTATA 
TTGGACCCTG TGCTTTCAAA TATGCAAGCA CAGAAATTAT TGCAGCTTAT TTGTTATCCT 
CATGGTATTA AAGAATGTAC GGAGGGCGAC AATCTGCAGA GACAGCACAT TAAGCGCATT 
CTTCAGAATC TTGAGCAGTG GACACTGAGG CAGTCCTGGC TAGAACTTCA GTTAATGATC 
AAACAGTGCT TGAAGGACCC TGGCTCTGGT TCAGTGGCTG AGATGAACAA CTTACTGGAC 
AATATTGCAA AGGCAACAAT AGAGGTATTC CAGCAGTCTG CTGACTTGAA CAATAATTCT 
TCTAATTCTG GAATGGGCCT CTTCAACCCA AATGGGATTG CAACTACTGA TGCTGGTAGC 
ACAAGGCAGA ATGGAATAAA GACATTTCTA AGTTCCTCTG AACGCAGGGG TGTATGGCTG 
GTGGCCCCCC TAATTGCCAG GCTGCCGACC TCTGTGCAAG GAAGAGTATT GAAAGCTGCA 
GGAGAGGAAC TGGAGAAGGG ACAGCACTTG GGCTCTTCTT CAAAAAAGGA AAGAGACAGA 
CAAAAACAGA AAAGTATGTC TCTTTTGAGT CAACAACCTT TCCTCTCACT GGTCCTTACC 
TGCCTTAAGG GACAAGATGA ACAACGGGAA GGCCTCCTAA CATCTCTTCA GAATCAGGTT 
AATCAGATTT TAAGTAATTG GAGAGAAGAA CGATACCAGG ATGACATAAA AGCACGGCAG 
ATGATGCATG AAGCATTGCA ACTTCGCCTA AATTTGGTAG GAGGAATGTT TGACACGGTG 
CAGAGGAGCA CCCAGTGGAC AACAGACTGG GCCCTCCTCC TGCTTCAGAT CATAACTTCT 
GGAACTGTTG ACATGCACAC TAACAATGAA TTATTCACAA CAGTTCTTGA CATGCTGGGT 
GTGTTAATCA ATGGAACATT AGCCTCAGAC CTATCAAATG CATCCCCAGG GGGATCTGAA 
GAGAACAAAC GTGCATACAT GAATTTAGTA AAGAAACTGA AGAAAGAGCT AGGAGACAAG 
AGATCAGAAA GTATTGACAA AGTTCGACAG TTGCTACCTT TGCCAAAACA GACATGTGAT 
GTCATTACTT GTGAACCTAT GGGTTCCTTG ATTGACACAA AGGGAAACAA AATTGCTGGA 
TTCGACTCCA TAGATAAAAA ACAGGGTCTT CAGGTGTCTA CAAAGCAGAA GGTGTCCCCA 
TGGGATTTGT TTGAGGGTCA GAAGAACCCA GCTCCTCTGT CTTGGGCCTG GTTCGGGACT 
GTCCGAGTGG ACCGAAAGGT GATCAAGTAC GAGGAGCAGC ATCACCTGCT GTTGTATCAC 
ACACACCCCA TGCCCAAGCC CCGAAGTTAC TACCTCGAAC CGCTGCCCCT GCCTCCCGAG 
GAGGAAGAGG AGGAGCCCAC ATCTCCCATC TCTCAGGAAC CAGAGCGGAA ATCAGCTGAG 
CTGTCAGATC AGGGAAAAAC CGCAACAGAT GAAGAGAAGA AAACAAAAGG AAGGAAGCGC 
AAGACGAAAT CAAGCTCAAG AGTTGACGAG TATCCACAGA GCAACATATA CCGAGTGCCT 
CCTAACTACT CACCCATTTC CTCCCAGATG ATGCACCATC CGCAGTCCAC CTTATGGGGC 
TATAACCTCA TGGGTCAGCC CCAGCAGCCG GGTTTTTTCC TTCAGAACCA ATCTCTTACT 
CCAGGTGGTT CTAGATTGGA CCCCACGGGC TCCTTCGTCC CAACGAATAC CAAACAAGCT 
CTGTCCAACA TGCTGCAGCG GCGCTCGGGG GCCATGATGC AGCCGCCGCC CCTCCACGCC 
ATCACATCGC AGCAGCAGCT GATACAGATG AAGCTTCTGC AGCAGCAGCA GCAGCAGCGG 
CTTCTCCGGC AAGCCCAGGC TCGACCTTTC CAGCAGGACT TCTGTAACTC TGCTGTCTCC 
CTTCTGGAAG ACTTTGACAA CATAATGGGC CCGCCAGGGG ACCAGGCTGC TCTCTTTGCT 
GCACAAGCAC GGCCCTCCCC TCAGCTCCCC CAGTATCCAG GGCTGCAGCA AGCACAGACC 
ATGCCCCAGG GCTATACGAT GTATGGAACG CAGATGCCTT TGCAGCAGAC CCCTCAGCAG 
CAGGCTGGTG GCGTAGTCCT GTCTCCTAGC TATAACTCCA GGACCTATCC GGCCACACAT 
TCCAACCCGG CGCTAATGGA AAGACTCAGA CAGATGCAGC AGCAGCCCAG TGGCTATGTT 
CAGCAGCAGG CGTCACCGTA CCTGCAGCCC TTGACCAGCT CTCAGAGACT AAATCATCAG 
TCGCTTCAGC AGAGCCCTCT GGTAGGTGGA GGAATCGATG CAGTGCTCAC TCCCACGCAT 
CCAAGCCTCC CCTCGGTGCC CCTGTCTCAG GACCCAATGA GAACCAGACA GCCGCAGGTT 
CGACAGCAGC AGAGACTCCT CCAGATGCAG CAGCCCCCCC AGCCCCAGCA GCCCCCGCAG 
CCCCAGAGTC AGACCCTTGG TCTCCAAGCG ATGCAGCCCC AGCAGCCTTT GTTTCCCAGG 
CAAGGCTTGC AGCAGACCCA GCAGCAGCAA CAGACGGCCG CCCTGGTGCG GCAGCTCCAG 
AAGCAGCTTT CTAGTAACCA GCCACAGCAA GGAGTGACTC CCTATGGGCA TCCATCACAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_019810561.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019666120.1 Felis catus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.

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CloneID	OFb48837
Clone ID Related Accession (Same CDS sequence)	XM_019810561.1
Accession Version	XM_019810561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTTCCTCAGG TTCCAATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCTAAATAT 
TCAGTGCCAA TGGTTCGAGC CACGTGGCTG ATCAAGATGA CGTGTGCCTA TTATTCTGCT 
ATTTCTGAAG CTAAAATTAA GAAACGTCAG GCTACTGATC CTAATTTGGA GTGGACACAA 
ATATCTACCA GATATCTTCG AGAGCAGCTG GCCAAGATTT CTGACTTTTA CCACATGGCT 
TCCAGCACAA GTGATGGTCC TGTCCCAGTG CCACCAGATG TGGAGCAAGC CATGAAGCAA 
TGGGAATACA ACGAAAAGTT GGCATTTCAC ATGTTCCAGG AAGGAATGCT AGAAAAACAT 
GAATATTTGA CATGGATCTT GGATGTGTTA GAGAAGATCA GACCGATGGA TGATGACCTT 
CTTAAACTCT TGTTACCACT CATGCTGCAG TATTCGGACG AGTTTGTTCA GTCGGCTTAC 
CTGTCTCGTC GTCTTGCCTA CTTTTGTGCC CGGCGTCTTG CCTTGCTGCT GAGCGATAGC 
CCCAGCCTCC TTGCTGCCCA CTCGCCCCAC ATGATGATAG GACCAAACAA CTCAAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GTCATGAGTC CCGTGCAGCT GGCCTTCTCA 
GACTTCCTCT CCTGTGCACA GCATGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTCACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ATTCCACAAA CGACAATAAG 
AGCGCGAACC CAGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG TCTCCCCATG 
CCGGGTGGGA ACACAGCTTT CAATCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCAACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAAGTGTT GGATCGACAT 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGATAA CCAAGAGGTC GCCCCCAACG ATGAAGCTGT GGTGACCTTG 
CTGTGTGAAT GGGCGGTGAG CTGCAAACGG TCAGGCAAGC ACAGGGCCAT GGCTGTGGCA 
AAACTCTTGG AGAAGAGGCA GGCGGAAATC GAAGCAGAGA GATGTGGTGA ATCAGAGGTC 
TTGGATGAGA AGGAGTCCAT TTCTTCAGCC TCTCTTGCTG GATCTAGTTT GCCTGTTTTC 
CAGAATGTGC TACTAAGGTT TTTAGATACA CAGGCCCCCT CATTATCGGA TCCAAACAGT 
GAATGTGAAA AAGTAGAATT TGTGAATTTG GTGCTGCTCT TCTGTGAGTT CATCCGACAC 
GATGTCTTCT CCCATGATGC GTACATGTGT ACCCTCATAT CTCGGGGAGA CTTGTCAGTC 
AGCGCCTCGA CTCGGCCGCG ATCGCCAGCA GGAGAAAACG TAGATGAGCA CTATCCAAAG 
GACCATGACA TGAAAATGGA GGAACAGACT ATTATGGCGC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAG 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAA TCCTGTGAGA ATGCCAACCC TTCCCTGGGC 
AGAAGAATGT CGGTTAATGG TGAGAAGTTG CTTAAGAGAG AAAAACCCAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT TCTGCGGCAC CTGCAGTATG CAACACATTT CCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTGCTTTA TGGAGTGGGC 
AAAGAGCGTG ACGAAGCGAA GCATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCACCAC AGAGACAGGG GTTGGAGATG AAGGACAAAA AACCAGGAAG 
AACAAACAGG AAGCATTTCC AACTCTGGAG ACTGTGTTCA CAAAACTTCA GCTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATCTCCAATA ATGTGCTGGA ACAAATCACA 
AGCTTCGCTT CAGGAACATC CTATCATCTC CCTTTGGCTC ACCATATTCA ACTCATCTTT 
GATCTTATGG AGCCAGCGCT GAACATCAAT GGACTAATTG ACTTCGCAAT ACAGCTGCTA 
AATGAACTGA GTGTTGTGGA AGCCGAACTG CTCCTGAAAT CCTCCAGCCT GGCAGGAAGT 
TACACGACGG GACTGTGTGT GTGCATTGTG GCTGTTCTCA GGCGGTACCA TAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGGT TGTGTGGTGT GGTAAAGCAT 
GTTGTGAACC CCTCAGAATG TTCTTCCCCC GAGAGATGCA TCTTAGCCTA CCTCTACGAT 
CTCTATGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTTA AGCAAACCAT ATATAATAAT GTGATGCCTG CAAATTCTAA CTTGAGATGG 
GATCCGGACT TCATGATGGA TTTTATTGAG AATCCTTCAG CTCGTAGCAT CAATTACTCA 
ATGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGTAACACG 
CTCATGAATG TATGTATGGG CCATCAGGAT GCTGGAAGGA TTAACGACAT AGCCAATTTC 
TCCTCTGAGC TGACAGCTTG CTGCACTGTT CTTAGTTCAG AATGGCTGGG AGTTCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGGTTTAATG ATGTACTTTG CACTGTAGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAG CACGTCGCAC TTCCCTCTCT CCTAGCAGCA 
GCTTGTGGAG ATGCAGATGC TGAGCCGGGG GCGAGAATGA CATGCCGACT CCTGCTTCAT 
CTCTTCCGAG CTCCCCAGGC CTGCTTGTTA CCTCAAGCAA CCGGTGAGCC GACAGCTATA 
AAATCCCCAA CTGCATGTTC ATTTGGAGGC AAACCTTTCC CTGGAATAAG ATCATCTTGT 
GATAGACACC TCTTAGCCGC CGCTCACAAT AGCATTGAAG TGGGAGCCGT GTTTGCTGTC 
TTAAAAGCAA TTATGATGCT TGGAGATGCC AAAATTGGCA ATAACAATGT CAGCTCTTTA 
AAGAATGATG ACTTCACCAT GAGAGGTTTA CGGCGTGATG GGAATGCTGA TGATATCTGG 
ACTGCTTCAC AAAATTCAAA ATCCTGTGGG AAAAGCATTT CCATAGAAAC TGCCAATTTA 
AGAGAATATG CTAGATATGT ACTGAGGACT ATCTGTCAAC AGGAATGGGT AGGAGAACAT 
TGCCTAAAAG AACCTGAAAG GTTGTGCACA GACAAAGAGC TTATATTGGA CCCTGTGCTT 
TCAAATATGC AAGCACAGAA ATTATTGCAG CTTATTTGTT ATCCTCATGG TATTAAAGAA 
TGTACGGAGG GCGACAATCT GCAGAGACAG CACATTAAGC GCATTCTTCA GAATCTTGAG 
CAGTGGACAC TGAGGCAGTC CTGGCTAGAA CTTCAGTTAA TGATCAAACA GTGCTTGAAG 
GACCCTGGCT CTGGTTCAGT GGCTGAGATG AACAACTTAC TGGACAATAT TGCAAAGGCA 
ACAATAGAGG TATTCCAGCA GTCTGCTGAC TTGAACAATA ATTCTTCTAA TTCTGGAATG 
GGCCTCTTCA ACCCAAATGG GATTGCAACT ACTGATGCTG GTAGCACAAG GCAGAATGGA 
ATAAAGACAT TTCTAAGTTC CTCTGAACGC AGGGGTGTAT GGCTGGTGGC CCCCCTAATT 
GCCAGGCTGC CGACCTCTGT GCAAGGAAGA GTATTGAAAG CTGCAGGAGA GGAACTGGAG 
AAGGGACAGC ACTTGGGCTC TTCTTCAAAA AAGGAAAGAG ACAGACAAAA ACAGAAAAGT 
ATGTCTCTTT TGAGTCAACA ACCTTTCCTC TCACTGGTCC TTACCTGCCT TAAGGGACAA 
GATGAACAAC GGGAAGGCCT CCTAACATCT CTTCAGAATC AGGTTAATCA GATTTTAAGT 
AATTGGAGAG AAGAACGATA CCAGGATGAC ATAAAAGCAC GGCAGATGAT GCATGAAGCA 
TTGCAACTTC GCCTAAATTT GGTAGGAGGA ATGTTTGACA CGGTGCAGAG GAGCACCCAG 
TGGACAACAG ACTGGGCCCT CCTCCTGCTT CAGATCATAA CTTCTGGAAC TGTTGACATG 
CACACTAACA ATGAATTATT CACAACAGTT CTTGACATGC TGGGTGTGTT AATCAATGGA 
ACATTAGCCT CAGACCTATC AAATGCATCC CCAGGGGGAT CTGAAGAGAA CAAACGTGCA 
TACATGAATT TAGTAAAGAA ACTGAAGAAA GAGCTAGGAG ACAAGAGATC AGAAAGTATT 
GACAAAGTTC GACAGTTGCT ACCTTTGCCA AAACAGACAT GTGATGTCAT TACTTGTGAA 
CCTATGGGTT CCTTGATTGA CACAAAGGGA AACAAAATTG CTGGATTCGA CTCCATAGAT 
AAAAAACAGG GTCTTCAGGT GTCTACAAAG CAGAAGGTGT CCCCATGGGA TTTGTTTGAG 
GGTCAGAAGA ACCCAGCTCC TCTGTCTTGG GCCTGGTTCG GGACTGTCCG AGTGGACCGA 
AAGGTGATCA AGTACGAGGA GCAGCATCAC CTGCTGTTGT ATCACACACA CCCCATGCCC 
AAGCCCCGAA GTTACTACCT CGAACCGCTG CCCCTGCCTC CCGAGGAGGA AGAGGAGGAG 
CCCACATCTC CCATCTCTCA GGAACCAGAG CGGAAATCAG CTGAGCTGTC AGATCAGGGA 
AAAACCGCAA CAGATGAAGA GAAGAAAACA AAAGGAAGGA AGCGCAAGAC GAAATCAAGC 
TCAAGAGTTG ACGAGTATCC ACAGAGCAAC ATATACCGAG TGCCTCCTAA CTACTCACCC 
ATTTCCTCCC AGATGATGCA CCATCCGCAG TCCACCTTAT GGGGCTATAA CCTCATGGGT 
CAGCCCCAGC AGCCGGGTTT TTTCCTTCAG AACCAATCTC TTACTCCAGG TGGTTCTAGA 
TTGGACCCCA CGGGCTCCTT CGTCCCAACG AATACCAAAC AAGCTCTGTC CAACATGCTG 
CAGCGGCGCT CGGGGGCCAT GATGCAGCCG CCGCCCCTCC ACGCCATCAC ATCGCAGCAG 
CAGCTGATAC AGATGAAGCT TCTGCAGCAG CAGCAGCAGC AGCGGCTTCT CCGGCAAGCC 
CAGGCTCGAC CTTTCCAGCA GGGCCCGCCA GGGGACCAGG CTGCTCTCTT TGCTGCACAA 
GCACGGCCCT CCCCTCAGCT CCCCCAGTAT CCAGGGCTGC AGCAAGCACA GACCATGCCC 
CAGGGCTATA CGATGTATGG AACGCAGATG CCTTTGCAGC AGACCCCTCA GCAGCAGGCT 
GGTGGCGTAG TCCTGTCTCC TAGCTATAAC TCCAGGACCT ATCCGGCCAC ACATTCCAAC 
CCGGCGCTAA TGGAAAGACT CAGACAGATG CAGCAGCAGC CCAGTGGCTA TGTTCAGCAG 
CAGGCGTCAC CGTACCTGCA GCCCTTGACC AGCTCTCAGA GACTAAATCA TCAGTCGCTT 
CAGCAGAGCC CTCTGGTAGG TGGAGGAATC GATGCAGTGC TCACTCCCAC GCATCCAAGC 
CTCCCCTCGG TGCCCCTGTC TCAGGACCCA ATGAGAACCA GACAGCCGCA GGTTCGACAG 
CAGCAGAGAC TCCTCCAGAT GCAGCAGCCC CCCCAGCCCC AGCAGCCCCC GCAGCCCCAG 
AGTCAGACCC TTGGTCTCCA AGCGATGCAG CCCCAGCAGC CTTTGTTTCC CAGGCAAGGC 
TTGCAGCAGA CCCAGCAGCA GCAACAGACG GCCGCCCTGG TGCGGCAGCT CCAGAAGCAG 
CTTTCTAGTA ACCAGCCACA GCAAGGAGTG ACTCCCTATG GGCATCCATC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666120.1
CDS	204..6803
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIAAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQATDPNLEWTQISTRYLREQLAKISDFYHMASSTSDGPVPVPPDVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHMMIGPNNSSIGAPSPGPPGPVMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVSASTRPRSPAGENVDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEAKHQLKKITKDILKILNKKSTTETGVGDEGQKTRKNKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGEPTAIKSPTACSFGGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNNVSSLKNDDFTMRGLRRDGNADDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNSGMGLFNPNGIATTDAGSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPMPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLMGQPQQPGFFLQNQSLTPGGSRLDPTGSFVPTNTKQALSNMLQRRSGAMMQPPPLHAITSQQQLIQMKLLQQQQQQRLLRQAQARPFQQGPPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGGVVLSPSYNSRTYPATHSNPALMERLRQMQQQPSGYVQQQASPYLQPLTSSQRLNHQSLQQSPLVGGGIDAVLTPTHPSLPSVPLSQDPMRTRQPQVRQQQRLLQMQQPPQPQQPPQPQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_019810561.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810561.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTTCCTCAGG TTCCAATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCTAAATAT 
TCAGTGCCAA TGGTTCGAGC CACGTGGCTG ATCAAGATGA CGTGTGCCTA TTATTCTGCT 
ATTTCTGAAG CTAAAATTAA GAAACGTCAG GCTACTGATC CTAATTTGGA GTGGACACAA 
ATATCTACCA GATATCTTCG AGAGCAGCTG GCCAAGATTT CTGACTTTTA CCACATGGCT 
TCCAGCACAA GTGATGGTCC TGTCCCAGTG CCACCAGATG TGGAGCAAGC CATGAAGCAA 
TGGGAATACA ACGAAAAGTT GGCATTTCAC ATGTTCCAGG AAGGAATGCT AGAAAAACAT 
GAATATTTGA CATGGATCTT GGATGTGTTA GAGAAGATCA GACCGATGGA TGATGACCTT 
CTTAAACTCT TGTTACCACT CATGCTGCAG TATTCGGACG AGTTTGTTCA GTCGGCTTAC 
CTGTCTCGTC GTCTTGCCTA CTTTTGTGCC CGGCGTCTTG CCTTGCTGCT GAGCGATAGC 
CCCAGCCTCC TTGCTGCCCA CTCGCCCCAC ATGATGATAG GACCAAACAA CTCAAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GTCATGAGTC CCGTGCAGCT GGCCTTCTCA 
GACTTCCTCT CCTGTGCACA GCATGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTCACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ATTCCACAAA CGACAATAAG 
AGCGCGAACC CAGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG TCTCCCCATG 
CCGGGTGGGA ACACAGCTTT CAATCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCAACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAAGTGTT GGATCGACAT 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGATAA CCAAGAGGTC GCCCCCAACG ATGAAGCTGT GGTGACCTTG 
CTGTGTGAAT GGGCGGTGAG CTGCAAACGG TCAGGCAAGC ACAGGGCCAT GGCTGTGGCA 
AAACTCTTGG AGAAGAGGCA GGCGGAAATC GAAGCAGAGA GATGTGGTGA ATCAGAGGTC 
TTGGATGAGA AGGAGTCCAT TTCTTCAGCC TCTCTTGCTG GATCTAGTTT GCCTGTTTTC 
CAGAATGTGC TACTAAGGTT TTTAGATACA CAGGCCCCCT CATTATCGGA TCCAAACAGT 
GAATGTGAAA AAGTAGAATT TGTGAATTTG GTGCTGCTCT TCTGTGAGTT CATCCGACAC 
GATGTCTTCT CCCATGATGC GTACATGTGT ACCCTCATAT CTCGGGGAGA CTTGTCAGTC 
AGCGCCTCGA CTCGGCCGCG ATCGCCAGCA GGAGAAAACG TAGATGAGCA CTATCCAAAG 
GACCATGACA TGAAAATGGA GGAACAGACT ATTATGGCGC ATATGGGCAT TGACTCAGGA 
ACTACTAATA TTTTTGATGA AGTAGACAAG AATGACTTTA AAACTGACTT TGGTTCGGAG 
TTTCCAATTT TTTCTCCCAT GCCTGGAGAA TCCTGTGAGA ATGCCAACCC TTCCCTGGGC 
AGAAGAATGT CGGTTAATGG TGAGAAGTTG CTTAAGAGAG AAAAACCCAG GGAGTTGATT 
TTTCCATCTA ATTATGACCT TCTGCGGCAC CTGCAGTATG CAACACATTT CCCCATACCT 
CTGGATGAAT CTTCAAGTCA TGAATGTAAC CAGCGCACAA TCCTGCTTTA TGGAGTGGGC 
AAAGAGCGTG ACGAAGCGAA GCATCAGCTG AAGAAGATTA CCAAAGATAT TTTGAAAATC 
CTAAATAAGA AGAGCACCAC AGAGACAGGG GTTGGAGATG AAGGACAAAA AACCAGGAAG 
AACAAACAGG AAGCATTTCC AACTCTGGAG ACTGTGTTCA CAAAACTTCA GCTCCTTTCA 
TATTTTGATC AGCATCAAGT GACATCTCAG ATCTCCAATA ATGTGCTGGA ACAAATCACA 
AGCTTCGCTT CAGGAACATC CTATCATCTC CCTTTGGCTC ACCATATTCA ACTCATCTTT 
GATCTTATGG AGCCAGCGCT GAACATCAAT GGACTAATTG ACTTCGCAAT ACAGCTGCTA 
AATGAACTGA GTGTTGTGGA AGCCGAACTG CTCCTGAAAT CCTCCAGCCT GGCAGGAAGT 
TACACGACGG GACTGTGTGT GTGCATTGTG GCTGTTCTCA GGCGGTACCA TAGTTGTCTG 
ATCCTGAATC CTGATCAGAC AGCCCAGGTG TTTGAAGGGT TGTGTGGTGT GGTAAAGCAT 
GTTGTGAACC CCTCAGAATG TTCTTCCCCC GAGAGATGCA TCTTAGCCTA CCTCTACGAT 
CTCTATGTGT CATGTAGCCA CCTCAGAAGT AAATTTGGAG ACCTCTTCAG TAGTGCCTGT 
TCAAAAGTTA AGCAAACCAT ATATAATAAT GTGATGCCTG CAAATTCTAA CTTGAGATGG 
GATCCGGACT TCATGATGGA TTTTATTGAG AATCCTTCAG CTCGTAGCAT CAATTACTCA 
ATGCTGGGCA AGATCCTTAG TGACAATGCA GCCAATCGCT ACAGCTTCGT CTGTAACACG 
CTCATGAATG TATGTATGGG CCATCAGGAT GCTGGAAGGA TTAACGACAT AGCCAATTTC 
TCCTCTGAGC TGACAGCTTG CTGCACTGTT CTTAGTTCAG AATGGCTGGG AGTTCTGAAG 
GCTCTTTGTT GTTCTTCAAA TCATGTGTGG GGGTTTAATG ATGTACTTTG CACTGTAGAT 
GTGAGTGACC TTTCATTCCA TGATTCATTA GCTACTTTCA TTGCTATTCT GATAGCACGA 
CAGTGTTTCT CCCTGGAGGA CGTCGTGCAG CACGTCGCAC TTCCCTCTCT CCTAGCAGCA 
GCTTGTGGAG ATGCAGATGC TGAGCCGGGG GCGAGAATGA CATGCCGACT CCTGCTTCAT 
CTCTTCCGAG CTCCCCAGGC CTGCTTGTTA CCTCAAGCAA CCGGTGAGCC GACAGCTATA 
AAATCCCCAA CTGCATGTTC ATTTGGAGGC AAACCTTTCC CTGGAATAAG ATCATCTTGT 
GATAGACACC TCTTAGCCGC CGCTCACAAT AGCATTGAAG TGGGAGCCGT GTTTGCTGTC 
TTAAAAGCAA TTATGATGCT TGGAGATGCC AAAATTGGCA ATAACAATGT CAGCTCTTTA 
AAGAATGATG ACTTCACCAT GAGAGGTTTA CGGCGTGATG GGAATGCTGA TGATATCTGG 
ACTGCTTCAC AAAATTCAAA ATCCTGTGGG AAAAGCATTT CCATAGAAAC TGCCAATTTA 
AGAGAATATG CTAGATATGT ACTGAGGACT ATCTGTCAAC AGGAATGGGT AGGAGAACAT 
TGCCTAAAAG AACCTGAAAG GTTGTGCACA GACAAAGAGC TTATATTGGA CCCTGTGCTT 
TCAAATATGC AAGCACAGAA ATTATTGCAG CTTATTTGTT ATCCTCATGG TATTAAAGAA 
TGTACGGAGG GCGACAATCT GCAGAGACAG CACATTAAGC GCATTCTTCA GAATCTTGAG 
CAGTGGACAC TGAGGCAGTC CTGGCTAGAA CTTCAGTTAA TGATCAAACA GTGCTTGAAG 
GACCCTGGCT CTGGTTCAGT GGCTGAGATG AACAACTTAC TGGACAATAT TGCAAAGGCA 
ACAATAGAGG TATTCCAGCA GTCTGCTGAC TTGAACAATA ATTCTTCTAA TTCTGGAATG 
GGCCTCTTCA ACCCAAATGG GATTGCAACT ACTGATGCTG GTAGCACAAG GCAGAATGGA 
ATAAAGACAT TTCTAAGTTC CTCTGAACGC AGGGGTGTAT GGCTGGTGGC CCCCCTAATT 
GCCAGGCTGC CGACCTCTGT GCAAGGAAGA GTATTGAAAG CTGCAGGAGA GGAACTGGAG 
AAGGGACAGC ACTTGGGCTC TTCTTCAAAA AAGGAAAGAG ACAGACAAAA ACAGAAAAGT 
ATGTCTCTTT TGAGTCAACA ACCTTTCCTC TCACTGGTCC TTACCTGCCT TAAGGGACAA 
GATGAACAAC GGGAAGGCCT CCTAACATCT CTTCAGAATC AGGTTAATCA GATTTTAAGT 
AATTGGAGAG AAGAACGATA CCAGGATGAC ATAAAAGCAC GGCAGATGAT GCATGAAGCA 
TTGCAACTTC GCCTAAATTT GGTAGGAGGA ATGTTTGACA CGGTGCAGAG GAGCACCCAG 
TGGACAACAG ACTGGGCCCT CCTCCTGCTT CAGATCATAA CTTCTGGAAC TGTTGACATG 
CACACTAACA ATGAATTATT CACAACAGTT CTTGACATGC TGGGTGTGTT AATCAATGGA 
ACATTAGCCT CAGACCTATC AAATGCATCC CCAGGGGGAT CTGAAGAGAA CAAACGTGCA 
TACATGAATT TAGTAAAGAA ACTGAAGAAA GAGCTAGGAG ACAAGAGATC AGAAAGTATT 
GACAAAGTTC GACAGTTGCT ACCTTTGCCA AAACAGACAT GTGATGTCAT TACTTGTGAA 
CCTATGGGTT CCTTGATTGA CACAAAGGGA AACAAAATTG CTGGATTCGA CTCCATAGAT 
AAAAAACAGG GTCTTCAGGT GTCTACAAAG CAGAAGGTGT CCCCATGGGA TTTGTTTGAG 
GGTCAGAAGA ACCCAGCTCC TCTGTCTTGG GCCTGGTTCG GGACTGTCCG AGTGGACCGA 
AAGGTGATCA AGTACGAGGA GCAGCATCAC CTGCTGTTGT ATCACACACA CCCCATGCCC 
AAGCCCCGAA GTTACTACCT CGAACCGCTG CCCCTGCCTC CCGAGGAGGA AGAGGAGGAG 
CCCACATCTC CCATCTCTCA GGAACCAGAG CGGAAATCAG CTGAGCTGTC AGATCAGGGA 
AAAACCGCAA CAGATGAAGA GAAGAAAACA AAAGGAAGGA AGCGCAAGAC GAAATCAAGC 
TCAAGAGTTG ACGAGTATCC ACAGAGCAAC ATATACCGAG TGCCTCCTAA CTACTCACCC 
ATTTCCTCCC AGATGATGCA CCATCCGCAG TCCACCTTAT GGGGCTATAA CCTCATGGGT 
CAGCCCCAGC AGCCGGGTTT TTTCCTTCAG AACCAATCTC TTACTCCAGG TGGTTCTAGA 
TTGGACCCCA CGGGCTCCTT CGTCCCAACG AATACCAAAC AAGCTCTGTC CAACATGCTG 
CAGCGGCGCT CGGGGGCCAT GATGCAGCCG CCGCCCCTCC ACGCCATCAC ATCGCAGCAG 
CAGCTGATAC AGATGAAGCT TCTGCAGCAG CAGCAGCAGC AGCGGCTTCT CCGGCAAGCC 
CAGGCTCGAC CTTTCCAGCA GGGCCCGCCA GGGGACCAGG CTGCTCTCTT TGCTGCACAA 
GCACGGCCCT CCCCTCAGCT CCCCCAGTAT CCAGGGCTGC AGCAAGCACA GACCATGCCC 
CAGGGCTATA CGATGTATGG AACGCAGATG CCTTTGCAGC AGACCCCTCA GCAGCAGGCT 
GGTGGCGTAG TCCTGTCTCC TAGCTATAAC TCCAGGACCT ATCCGGCCAC ACATTCCAAC 
CCGGCGCTAA TGGAAAGACT CAGACAGATG CAGCAGCAGC CCAGTGGCTA TGTTCAGCAG 
CAGGCGTCAC CGTACCTGCA GCCCTTGACC AGCTCTCAGA GACTAAATCA TCAGTCGCTT 
CAGCAGAGCC CTCTGGTAGG TGGAGGAATC GATGCAGTGC TCACTCCCAC GCATCCAAGC 
CTCCCCTCGG TGCCCCTGTC TCAGGACCCA ATGAGAACCA GACAGCCGCA GGTTCGACAG 
CAGCAGAGAC TCCTCCAGAT GCAGCAGCCC CCCCAGCCCC AGCAGCCCCC GCAGCCCCAG 
AGTCAGACCC TTGGTCTCCA AGCGATGCAG CCCCAGCAGC CTTTGTTTCC CAGGCAAGGC 
TTGCAGCAGA CCCAGCAGCA GCAACAGACG GCCGCCCTGG TGCGGCAGCT CCAGAAGCAG 
CTTTCTAGTA ACCAGCCACA GCAAGGAGTG ACTCCCTATG GGCATCCATC ACACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_019810562.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019666121.1 Felis catus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

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CloneID	OFb48838
Clone ID Related Accession (Same CDS sequence)	XM_019810562.1
Accession Version	XM_019810562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGGA ACAGACTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACT ACTAATATTT TTGATGAAGT AGACAAGAAT 
GACTTTAAAA CTGACTTTGG TTCGGAGTTT CCAATTTTTT CTCCCATGCC TGGAGAATCC 
TGTGAGAATG CCAACCCTTC CCTGGGCAGA AGAATGTCGG TTAATGGTGA GAAGTTGCTT 
AAGAGAGAAA AACCCAGGGA GTTGATTTTT CCATCTAATT ATGACCTTCT GCGGCACCTG 
CAGTATGCAA CACATTTCCC CATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TGCTTTATGG AGTGGGCAAA GAGCGTGACG AAGCGAAGCA TCAGCTGAAG 
AAGATTACCA AAGATATTTT GAAAATCCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGAGATGAAG GACAAAAAAC CAGGAAGAAC AAACAGGAAG CATTTCCAAC TCTGGAGACT 
GTGTTCACAA AACTTCAGCT CCTTTCATAT TTTGATCAGC ATCAAGTGAC ATCTCAGATC 
TCCAATAATG TGCTGGAACA AATCACAAGC TTCGCTTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ATATTCAACT CATCTTTGAT CTTATGGAGC CAGCGCTGAA CATCAATGGA 
CTAATTGACT TCGCAATACA GCTGCTAAAT GAACTGAGTG TTGTGGAAGC CGAACTGCTC 
CTGAAATCCT CCAGCCTGGC AGGAAGTTAC ACGACGGGAC TGTGTGTGTG CATTGTGGCT 
GTTCTCAGGC GGTACCATAG TTGTCTGATC CTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT AAAGCATGTT GTGAACCCCT CAGAATGTTC TTCCCCCGAG 
AGATGCATCT TAGCCTACCT CTACGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTTAAGC AAACCATATA TAATAATGTG 
ATGCCTGCAA ATTCTAACTT GAGATGGGAT CCGGACTTCA TGATGGATTT TATTGAGAAT 
CCTTCAGCTC GTAGCATCAA TTACTCAATG CTGGGCAAGA TCCTTAGTGA CAATGCAGCC 
AATCGCTACA GCTTCGTCTG TAACACGCTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGAAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTGA CAGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGAGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA TGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTCTCCC TGGAGGACGT CGTGCAGCAC 
GTCGCACTTC CCTCTCTCCT AGCAGCAGCT TGTGGAGATG CAGATGCTGA GCCGGGGGCG 
AGAATGACAT GCCGACTCCT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTGTTACCT 
CAAGCAACCG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCCGCTCACA ATAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAAT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TACGGCGTGA TGGGAATGCT GATGATATCT GGACTGCTTC ACAAAATTCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA TGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAAC ATTGCCTAAA AGAACCTGAA 
AGGTTGTGCA CAGACAAAGA GCTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTATTGC AGCTTATTTG TTATCCTCAT GGTATTAAAG AATGTACGGA GGGCGACAAT 
CTGCAGAGAC AGCACATTAA GCGCATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAG 
TCCTGGCTAG AACTTCAGTT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCA 
GTGGCTGAGA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCTG ACTTGAACAA TAATTCTTCT AATTCTGGAA TGGGCCTCTT CAACCCAAAT 
GGGATTGCAA CTACTGATGC TGGTAGCACA AGGCAGAATG GAATAAAGAC ATTTCTAAGT 
TCCTCTGAAC GCAGGGGTGT ATGGCTGGTG GCCCCCCTAA TTGCCAGGCT GCCGACCTCT 
GTGCAAGGAA GAGTATTGAA AGCTGCAGGA GAGGAACTGG AGAAGGGACA GCACTTGGGC 
TCTTCTTCAA AAAAGGAAAG AGACAGACAA AAACAGAAAA GTATGTCTCT TTTGAGTCAA 
CAACCTTTCC TCTCACTGGT CCTTACCTGC CTTAAGGGAC AAGATGAACA ACGGGAAGGC 
CTCCTAACAT CTCTTCAGAA TCAGGTTAAT CAGATTTTAA GTAATTGGAG AGAAGAACGA 
TACCAGGATG ACATAAAAGC ACGGCAGATG ATGCATGAAG CATTGCAACT TCGCCTAAAT 
TTGGTAGGAG GAATGTTTGA CACGGTGCAG AGGAGCACCC AGTGGACAAC AGACTGGGCC 
CTCCTCCTGC TTCAGATCAT AACTTCTGGA ACTGTTGACA TGCACACTAA CAATGAATTA 
TTCACAACAG TTCTTGACAT GCTGGGTGTG TTAATCAATG GAACATTAGC CTCAGACCTA 
TCAAATGCAT CCCCAGGGGG ATCTGAAGAG AACAAACGTG CATACATGAA TTTAGTAAAG 
AAACTGAAGA AAGAGCTAGG AGACAAGAGA TCAGAAAGTA TTGACAAAGT TCGACAGTTG 
CTACCTTTGC CAAAACAGAC ATGTGATGTC ATTACTTGTG AACCTATGGG TTCCTTGATT 
GACACAAAGG GAAACAAAAT TGCTGGATTC GACTCCATAG ATAAAAAACA GGGTCTTCAG 
GTGTCTACAA AGCAGAAGGT GTCCCCATGG GATTTGTTTG AGGGTCAGAA GAACCCAGCT 
CCTCTGTCTT GGGCCTGGTT CGGGACTGTC CGAGTGGACC GAAAGGTGAT CAAGTACGAG 
GAGCAGCATC ACCTGCTGTT GTATCACACA CACCCCATGC CCAAGCCCCG AAGTTACTAC 
CTCGAACCGC TGCCCCTGCC TCCCGAGGAG GAAGAGGAGG AGCCCACATC TCCCATCTCT 
CAGGAACCAG AGCGGAAATC AGCTGAGCTG TCAGATCAGG GAAAAACCGC AACAGATGAA 
GAGAAGAAAA CAAAAGGAAG GAAGCGCAAG ACGAAATCAA GCTCAAGAGT TGACGAGTAT 
CCACAGAGCA ACATATACCG AGTGCCTCCT AACTACTCAC CCATTTCCTC CCAGATGATG 
CACCATCCGC AGTCCACCTT ATGGGGCTAT AACCTCATGG GTCAGCCCCA GCAGCCGGGT 
TTTTTCCTTC AGAACCAATC TCTTACTCCA GGTGGTTCTA GATTGGACCC CACGGGCTCC 
TTCGTCCCAA CGAATACCAA ACAAGCTCTG TCCAACATGC TGCAGCGGCG CTCGGGGGCC 
ATGATGCAGC CGCCGCCCCT CCACGCCATC ACATCGCAGC AGCAGCTGAT ACAGATGAAG 
CTTCTGCAGC AGCAGCAGCA GCAGCGGCTT CTCCGGCAAG CCCAGGCTCG ACCTTTCCAG 
CAGGGCCCGC CAGGGGACCA GGCTGCTCTC TTTGCTGCAC AAGCACGGCC CTCCCCTCAG 
CTCCCCCAGT ATCCAGGGCT GCAGCAAGCA CAGACCATGC CCCAGGGCTA TACGATGTAT 
GGAACGCAGA TGCCTTTGCA GCAGACCCCT CAGCAGCAGG CTGGTGGCGT AGTCCTGTCT 
CCTAGCTATA ACTCCAGGAC CTATCCGGCC ACACATTCCA ACCCGGCGCT AATGGAAAGA 
CTCAGACAGA TGCAGCAGCA GCCCAGTGGC TATGTTCAGC AGCAGGCGTC ACCGTACCTG 
CAGCCCTTGA CCAGCTCTCA GAGACTAAAT CATCAGTCGC TTCAGCAGAG CCCTCTGGTA 
GGTGGAGGAA TCGATGCAGT GCTCACTCCC ACGCATCCAA GCCTCCCCTC GGTGCCCCTG 
TCTCAGGACC CAATGAGAAC CAGACAGCCG CAGGTTCGAC AGCAGCAGAG ACTCCTCCAG 
ATGCAGCAGC CCCCCCAGCC CCAGCAGCCC CCGCAGCCCC AGAGTCAGAC CCTTGGTCTC 
CAAGCGATGC AGCCCCAGCA GCCTTTGTTT CCCAGGCAAG GCTTGCAGCA GACCCAGCAG 
CAGCAACAGA CGGCCGCCCT GGTGCGGCAG CTCCAGAAGC AGCTTTCTAG TAACCAGCCA 
CAGCAAGGAG TGACTCCCTA TGGGCATCCA TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666121.1
CDS	1..6522
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIAAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQATDPNLEWTQISTRYLREQLAKISDFYHMASSTSDGPVPVPPDVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHMMIGPNNSSIGAPSPGPPGPVMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVSASTRPRSPAGENVDEHYPKDHDMKMEEQTIMAHMGIDSGTTNIFDEVDKNDFKTDFGSEFPIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEAKHQLKKITKDILKILNKKSTTETGVGDEGQKTRKNKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNNVSSLKNDDFTMRGLRRDGNADDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNSGMGLFNPNGIATTDAGSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPMPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLMGQPQQPGFFLQNQSLTPGGSRLDPTGSFVPTNTKQALSNMLQRRSGAMMQPPPLHAITSQQQLIQMKLLQQQQQQRLLRQAQARPFQQGPPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGGVVLSPSYNSRTYPATHSNPALMERLRQMQQQPSGYVQQQASPYLQPLTSSQRLNHQSLQQSPLVGGGIDAVLTPTHPSLPSVPLSQDPMRTRQPQVRQQQRLLQMQQPPQPQQPPQPQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_019810562.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810562.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGGA ACAGACTATT 
ATGGCGCATA TGGGCATTGA CTCAGGAACT ACTAATATTT TTGATGAAGT AGACAAGAAT 
GACTTTAAAA CTGACTTTGG TTCGGAGTTT CCAATTTTTT CTCCCATGCC TGGAGAATCC 
TGTGAGAATG CCAACCCTTC CCTGGGCAGA AGAATGTCGG TTAATGGTGA GAAGTTGCTT 
AAGAGAGAAA AACCCAGGGA GTTGATTTTT CCATCTAATT ATGACCTTCT GCGGCACCTG 
CAGTATGCAA CACATTTCCC CATACCTCTG GATGAATCTT CAAGTCATGA ATGTAACCAG 
CGCACAATCC TGCTTTATGG AGTGGGCAAA GAGCGTGACG AAGCGAAGCA TCAGCTGAAG 
AAGATTACCA AAGATATTTT GAAAATCCTA AATAAGAAGA GCACCACAGA GACAGGGGTT 
GGAGATGAAG GACAAAAAAC CAGGAAGAAC AAACAGGAAG CATTTCCAAC TCTGGAGACT 
GTGTTCACAA AACTTCAGCT CCTTTCATAT TTTGATCAGC ATCAAGTGAC ATCTCAGATC 
TCCAATAATG TGCTGGAACA AATCACAAGC TTCGCTTCAG GAACATCCTA TCATCTCCCT 
TTGGCTCACC ATATTCAACT CATCTTTGAT CTTATGGAGC CAGCGCTGAA CATCAATGGA 
CTAATTGACT TCGCAATACA GCTGCTAAAT GAACTGAGTG TTGTGGAAGC CGAACTGCTC 
CTGAAATCCT CCAGCCTGGC AGGAAGTTAC ACGACGGGAC TGTGTGTGTG CATTGTGGCT 
GTTCTCAGGC GGTACCATAG TTGTCTGATC CTGAATCCTG ATCAGACAGC CCAGGTGTTT 
GAAGGGTTGT GTGGTGTGGT AAAGCATGTT GTGAACCCCT CAGAATGTTC TTCCCCCGAG 
AGATGCATCT TAGCCTACCT CTACGATCTC TATGTGTCAT GTAGCCACCT CAGAAGTAAA 
TTTGGAGACC TCTTCAGTAG TGCCTGTTCA AAAGTTAAGC AAACCATATA TAATAATGTG 
ATGCCTGCAA ATTCTAACTT GAGATGGGAT CCGGACTTCA TGATGGATTT TATTGAGAAT 
CCTTCAGCTC GTAGCATCAA TTACTCAATG CTGGGCAAGA TCCTTAGTGA CAATGCAGCC 
AATCGCTACA GCTTCGTCTG TAACACGCTC ATGAATGTAT GTATGGGCCA TCAGGATGCT 
GGAAGGATTA ACGACATAGC CAATTTCTCC TCTGAGCTGA CAGCTTGCTG CACTGTTCTT 
AGTTCAGAAT GGCTGGGAGT TCTGAAGGCT CTTTGTTGTT CTTCAAATCA TGTGTGGGGG 
TTTAATGATG TACTTTGCAC TGTAGATGTG AGTGACCTTT CATTCCATGA TTCATTAGCT 
ACTTTCATTG CTATTCTGAT AGCACGACAG TGTTTCTCCC TGGAGGACGT CGTGCAGCAC 
GTCGCACTTC CCTCTCTCCT AGCAGCAGCT TGTGGAGATG CAGATGCTGA GCCGGGGGCG 
AGAATGACAT GCCGACTCCT GCTTCATCTC TTCCGAGCTC CCCAGGCCTG CTTGTTACCT 
CAAGCAACCG GCAAACCTTT CCCTGGAATA AGATCATCTT GTGATAGACA CCTCTTAGCC 
GCCGCTCACA ATAGCATTGA AGTGGGAGCC GTGTTTGCTG TCTTAAAAGC AATTATGATG 
CTTGGAGATG CCAAAATTGG CAATAACAAT GTCAGCTCTT TAAAGAATGA TGACTTCACC 
ATGAGAGGTT TACGGCGTGA TGGGAATGCT GATGATATCT GGACTGCTTC ACAAAATTCA 
AAATCCTGTG GGAAAAGCAT TTCCATAGAA ACTGCCAATT TAAGAGAATA TGCTAGATAT 
GTACTGAGGA CTATCTGTCA ACAGGAATGG GTAGGAGAAC ATTGCCTAAA AGAACCTGAA 
AGGTTGTGCA CAGACAAAGA GCTTATATTG GACCCTGTGC TTTCAAATAT GCAAGCACAG 
AAATTATTGC AGCTTATTTG TTATCCTCAT GGTATTAAAG AATGTACGGA GGGCGACAAT 
CTGCAGAGAC AGCACATTAA GCGCATTCTT CAGAATCTTG AGCAGTGGAC ACTGAGGCAG 
TCCTGGCTAG AACTTCAGTT AATGATCAAA CAGTGCTTGA AGGACCCTGG CTCTGGTTCA 
GTGGCTGAGA TGAACAACTT ACTGGACAAT ATTGCAAAGG CAACAATAGA GGTATTCCAG 
CAGTCTGCTG ACTTGAACAA TAATTCTTCT AATTCTGGAA TGGGCCTCTT CAACCCAAAT 
GGGATTGCAA CTACTGATGC TGGTAGCACA AGGCAGAATG GAATAAAGAC ATTTCTAAGT 
TCCTCTGAAC GCAGGGGTGT ATGGCTGGTG GCCCCCCTAA TTGCCAGGCT GCCGACCTCT 
GTGCAAGGAA GAGTATTGAA AGCTGCAGGA GAGGAACTGG AGAAGGGACA GCACTTGGGC 
TCTTCTTCAA AAAAGGAAAG AGACAGACAA AAACAGAAAA GTATGTCTCT TTTGAGTCAA 
CAACCTTTCC TCTCACTGGT CCTTACCTGC CTTAAGGGAC AAGATGAACA ACGGGAAGGC 
CTCCTAACAT CTCTTCAGAA TCAGGTTAAT CAGATTTTAA GTAATTGGAG AGAAGAACGA 
TACCAGGATG ACATAAAAGC ACGGCAGATG ATGCATGAAG CATTGCAACT TCGCCTAAAT 
TTGGTAGGAG GAATGTTTGA CACGGTGCAG AGGAGCACCC AGTGGACAAC AGACTGGGCC 
CTCCTCCTGC TTCAGATCAT AACTTCTGGA ACTGTTGACA TGCACACTAA CAATGAATTA 
TTCACAACAG TTCTTGACAT GCTGGGTGTG TTAATCAATG GAACATTAGC CTCAGACCTA 
TCAAATGCAT CCCCAGGGGG ATCTGAAGAG AACAAACGTG CATACATGAA TTTAGTAAAG 
AAACTGAAGA AAGAGCTAGG AGACAAGAGA TCAGAAAGTA TTGACAAAGT TCGACAGTTG 
CTACCTTTGC CAAAACAGAC ATGTGATGTC ATTACTTGTG AACCTATGGG TTCCTTGATT 
GACACAAAGG GAAACAAAAT TGCTGGATTC GACTCCATAG ATAAAAAACA GGGTCTTCAG 
GTGTCTACAA AGCAGAAGGT GTCCCCATGG GATTTGTTTG AGGGTCAGAA GAACCCAGCT 
CCTCTGTCTT GGGCCTGGTT CGGGACTGTC CGAGTGGACC GAAAGGTGAT CAAGTACGAG 
GAGCAGCATC ACCTGCTGTT GTATCACACA CACCCCATGC CCAAGCCCCG AAGTTACTAC 
CTCGAACCGC TGCCCCTGCC TCCCGAGGAG GAAGAGGAGG AGCCCACATC TCCCATCTCT 
CAGGAACCAG AGCGGAAATC AGCTGAGCTG TCAGATCAGG GAAAAACCGC AACAGATGAA 
GAGAAGAAAA CAAAAGGAAG GAAGCGCAAG ACGAAATCAA GCTCAAGAGT TGACGAGTAT 
CCACAGAGCA ACATATACCG AGTGCCTCCT AACTACTCAC CCATTTCCTC CCAGATGATG 
CACCATCCGC AGTCCACCTT ATGGGGCTAT AACCTCATGG GTCAGCCCCA GCAGCCGGGT 
TTTTTCCTTC AGAACCAATC TCTTACTCCA GGTGGTTCTA GATTGGACCC CACGGGCTCC 
TTCGTCCCAA CGAATACCAA ACAAGCTCTG TCCAACATGC TGCAGCGGCG CTCGGGGGCC 
ATGATGCAGC CGCCGCCCCT CCACGCCATC ACATCGCAGC AGCAGCTGAT ACAGATGAAG 
CTTCTGCAGC AGCAGCAGCA GCAGCGGCTT CTCCGGCAAG CCCAGGCTCG ACCTTTCCAG 
CAGGGCCCGC CAGGGGACCA GGCTGCTCTC TTTGCTGCAC AAGCACGGCC CTCCCCTCAG 
CTCCCCCAGT ATCCAGGGCT GCAGCAAGCA CAGACCATGC CCCAGGGCTA TACGATGTAT 
GGAACGCAGA TGCCTTTGCA GCAGACCCCT CAGCAGCAGG CTGGTGGCGT AGTCCTGTCT 
CCTAGCTATA ACTCCAGGAC CTATCCGGCC ACACATTCCA ACCCGGCGCT AATGGAAAGA 
CTCAGACAGA TGCAGCAGCA GCCCAGTGGC TATGTTCAGC AGCAGGCGTC ACCGTACCTG 
CAGCCCTTGA CCAGCTCTCA GAGACTAAAT CATCAGTCGC TTCAGCAGAG CCCTCTGGTA 
GGTGGAGGAA TCGATGCAGT GCTCACTCCC ACGCATCCAA GCCTCCCCTC GGTGCCCCTG 
TCTCAGGACC CAATGAGAAC CAGACAGCCG CAGGTTCGAC AGCAGCAGAG ACTCCTCCAG 
ATGCAGCAGC CCCCCCAGCC CCAGCAGCCC CCGCAGCCCC AGAGTCAGAC CCTTGGTCTC 
CAAGCGATGC AGCCCCAGCA GCCTTTGTTT CCCAGGCAAG GCTTGCAGCA GACCCAGCAG 
CAGCAACAGA CGGCCGCCCT GGTGCGGCAG CTCCAGAAGC AGCTTTCTAG TAACCAGCCA 
CAGCAAGGAG TGACTCCCTA TGGGCATCCA TCACACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_019810563.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019666122.1 Felis catus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

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CloneID	OFb48839
Clone ID Related Accession (Same CDS sequence)	XM_019810563.1
Accession Version	XM_019810563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTTCCTCAGG TTCCAATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCTAAATAT 
TCAGTGCCAA TGGTTCGAGC CACGTGGCTG ATCAAGATGA CGTGTGCCTA TTATTCTGCT 
ATTTCTGAAG CTAAAATTAA GAAACGTCAG GCTACTGATC CTAATTTGGA GTGGACACAA 
ATATCTACCA GATATCTTCG AGAGCAGCTG GCCAAGATTT CTGACTTTTA CCACATGGCT 
TCCAGCACAA GTGATGGTCC TGTCCCAGTG CCACCAGATG TGGAGCAAGC CATGAAGCAA 
TGGGAATACA ACGAAAAGTT GGCATTTCAC ATGTTCCAGG AAGGAATGCT AGAAAAACAT 
GAATATTTGA CATGGATCTT GGATGTGTTA GAGAAGATCA GACCGATGGA TGATGACCTT 
CTTAAACTCT TGTTACCACT CATGCTGCAG TATTCGGACG AGTTTGTTCA GTCGGCTTAC 
CTGTCTCGTC GTCTTGCCTA CTTTTGTGCC CGGCGTCTTG CCTTGCTGCT GAGCGATAGC 
CCCAGCCTCC TTGCTGCCCA CTCGCCCCAC ATGATGATAG GACCAAACAA CTCAAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GTCATGAGTC CCGTGCAGCT GGCCTTCTCA 
GACTTCCTCT CCTGTGCACA GCATGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTCACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ATTCCACAAA CGACAATAAG 
AGCGCGAACC CAGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG TCTCCCCATG 
CCGGGTGGGA ACACAGCTTT CAATCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCAACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAAGTGTT GGATCGACAT 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGATAA CCAAGAGGTC GCCCCCAACG ATGAAGCTGT GGTGACCTTG 
CTGTGTGAAT GGGCGGTGAG CTGCAAACGG TCAGGCAAGC ACAGGGCCAT GGCTGTGGCA 
AAACTCTTGG AGAAGAGGCA GGCGGAAATC GAAGCAGAGA GATGTGGTGA ATCAGAGGTC 
TTGGATGAGA AGGAGTCCAT TTCTTCAGCC TCTCTTGCTG GATCTAGTTT GCCTGTTTTC 
CAGAATGTGC TACTAAGGTT TTTAGATACA CAGGCCCCCT CATTATCGGA TCCAAACAGT 
GAATGTGAAA AAGTAGAATT TGTGAATTTG GTGCTGCTCT TCTGTGAGTT CATCCGACAC 
GATGTCTTCT CCCATGATGC GTACATGTGT ACCCTCATAT CTCGGGGAGA CTTGTCAGTC 
AGCGCCTCGA CTCGGCCGCG ATCGCCAGCA GGAGAAAACG TAGATGAGCA CTATCCAAAG 
GACCATGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAATCCTG TGAGAATGCC 
AACCCTTCCC TGGGCAGAAG AATGTCGGTT AATGGTGAGA AGTTGCTTAA GAGAGAAAAA 
CCCAGGGAGT TGATTTTTCC ATCTAATTAT GACCTTCTGC GGCACCTGCA GTATGCAACA 
CATTTCCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTG 
CTTTATGGAG TGGGCAAAGA GCGTGACGAA GCGAAGCATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC ACCACAGAGA CAGGGGTTGG AGATGAAGGA 
CAAAAAACCA GGAAGAACAA ACAGGAAGCA TTTCCAACTC TGGAGACTGT GTTCACAAAA 
CTTCAGCTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATCTC CAATAATGTG 
CTGGAACAAA TCACAAGCTT CGCTTCAGGA ACATCCTATC ATCTCCCTTT GGCTCACCAT 
ATTCAACTCA TCTTTGATCT TATGGAGCCA GCGCTGAACA TCAATGGACT AATTGACTTC 
GCAATACAGC TGCTAAATGA ACTGAGTGTT GTGGAAGCCG AACTGCTCCT GAAATCCTCC 
AGCCTGGCAG GAAGTTACAC GACGGGACTG TGTGTGTGCA TTGTGGCTGT TCTCAGGCGG 
TACCATAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGGTTGTGT 
GGTGTGGTAA AGCATGTTGT GAACCCCTCA GAATGTTCTT CCCCCGAGAG ATGCATCTTA 
GCCTACCTCT ACGATCTCTA TGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTTAAGCAA ACCATATATA ATAATGTGAT GCCTGCAAAT 
TCTAACTTGA GATGGGATCC GGACTTCATG ATGGATTTTA TTGAGAATCC TTCAGCTCGT 
AGCATCAATT ACTCAATGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGTA ACACGCTCAT GAATGTATGT ATGGGCCATC AGGATGCTGG AAGGATTAAC 
GACATAGCCA ATTTCTCCTC TGAGCTGACA GCTTGCTGCA CTGTTCTTAG TTCAGAATGG 
CTGGGAGTTC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGGTT TAATGATGTA 
CTTTGCACTG TAGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAGCACGT CGCACTTCCC 
TCTCTCCTAG CAGCAGCTTG TGGAGATGCA GATGCTGAGC CGGGGGCGAG AATGACATGC 
CGACTCCTGC TTCATCTCTT CCGAGCTCCC CAGGCCTGCT TGTTACCTCA AGCAACCGGC 
AAACCTTTCC CTGGAATAAG ATCATCTTGT GATAGACACC TCTTAGCCGC CGCTCACAAT 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAATGT CAGCTCTTTA AAGAATGATG ACTTCACCAT GAGAGGTTTA 
CGGCGTGATG GGAATGCTGA TGATATCTGG ACTGCTTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC TGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACT 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGCCTAAAAG AACCTGAAAG GTTGTGCACA 
GACAAAGAGC TTATATTGGA CCCTGTGCTT TCAAATATGC AAGCACAGAA ATTATTGCAG 
CTTATTTGTT ATCCTCATGG TATTAAAGAA TGTACGGAGG GCGACAATCT GCAGAGACAG 
CACATTAAGC GCATTCTTCA GAATCTTGAG CAGTGGACAC TGAGGCAGTC CTGGCTAGAA 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCAGT GGCTGAGATG 
AACAACTTAC TGGACAATAT TGCAAAGGCA ACAATAGAGG TATTCCAGCA GTCTGCTGAC 
TTGAACAATA ATTCTTCTAA TTCTGGAATG GGCCTCTTCA ACCCAAATGG GATTGCAACT 
ACTGATGCTG GTAGCACAAG GCAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTAT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACCTCTGT GCAAGGAAGA 
GTATTGAAAG CTGCAGGAGA GGAACTGGAG AAGGGACAGC ACTTGGGCTC TTCTTCAAAA 
AAGGAAAGAG ACAGACAAAA ACAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCTTTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GGGAAGGCCT CCTAACATCT 
CTTCAGAATC AGGTTAATCA GATTTTAAGT AATTGGAGAG AAGAACGATA CCAGGATGAC 
ATAAAAGCAC GGCAGATGAT GCATGAAGCA TTGCAACTTC GCCTAAATTT GGTAGGAGGA 
ATGTTTGACA CGGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCCCT CCTCCTGCTT 
CAGATCATAA CTTCTGGAAC TGTTGACATG CACACTAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTGTT AATCAATGGA ACATTAGCCT CAGACCTATC AAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAATT TAGTAAAGAA ACTGAAGAAA 
GAGCTAGGAG ACAAGAGATC AGAAAGTATT GACAAAGTTC GACAGTTGCT ACCTTTGCCA 
AAACAGACAT GTGATGTCAT TACTTGTGAA CCTATGGGTT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA CTCCATAGAT AAAAAACAGG GTCTTCAGGT GTCTACAAAG 
CAGAAGGTGT CCCCATGGGA TTTGTTTGAG GGTCAGAAGA ACCCAGCTCC TCTGTCTTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGA AAGGTGATCA AGTACGAGGA GCAGCATCAC 
CTGCTGTTGT ATCACACACA CCCCATGCCC AAGCCCCGAA GTTACTACCT CGAACCGCTG 
CCCCTGCCTC CCGAGGAGGA AGAGGAGGAG CCCACATCTC CCATCTCTCA GGAACCAGAG 
CGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAAACA 
AAAGGAAGGA AGCGCAAGAC GAAATCAAGC TCAAGAGTTG ACGAGTATCC ACAGAGCAAC 
ATATACCGAG TGCCTCCTAA CTACTCACCC ATTTCCTCCC AGATGATGCA CCATCCGCAG 
TCCACCTTAT GGGGCTATAA CCTCATGGGT CAGCCCCAGC AGCCGGGTTT TTTCCTTCAG 
AACCAATCTC TTACTCCAGG TGGTTCTAGA TTGGACCCCA CGGGCTCCTT CGTCCCAACG 
AATACCAAAC AAGCTCTGTC CAACATGCTG CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCGCCCCTCC ACGCCATCAC ATCGCAGCAG CAGCTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAGC AGCGGCTTCT CCGGCAAGCC CAGGCTCGAC CTTTCCAGCA GGACTTCTGT 
AACTCTGCTG TCTCCCTTCT GGAAGACTTT GACAACATAA TGGGCCCGCC AGGGGACCAG 
GCTGCTCTCT TTGCTGCACA AGCACGGCCC TCCCCTCAGC TCCCCCAGTA TCCAGGGCTG 
CAGCAAGCAC AGACCATGCC CCAGGGCTAT ACGATGTATG GAACGCAGAT GCCTTTGCAG 
CAGACCCCTC AGCAGCAGGC TGGTGGCGTA GTCCTGTCTC CTAGCTATAA CTCCAGGACC 
TATCCGGCCA CACATTCCAA CCCGGCGCTA ATGGAAAGAC TCAGACAGAT GCAGCAGCAG 
CCCAGTGGCT ATGTTCAGCA GCAGGCGTCA CCGTACCTGC AGCCCTTGAC CAGCTCTCAG 
AGACTAAATC ATCAGTCGCT TCAGCAGAGC CCTCTGGTAG GTGGAGGAAT CGATGCAGTG 
CTCACTCCCA CGCATCCAAG CCTCCCCTCG GTGCCCCTGT CTCAGGACCC AATGAGAACC 
AGACAGCCGC AGGTTCGACA GCAGCAGAGA CTCCTCCAGA TGCAGCAGCC CCCCCAGCCC 
CAGCAGCCCC CGCAGCCCCA GAGTCAGACC CTTGGTCTCC AAGCGATGCA GCCCCAGCAG 
CCTTTGTTTC CCAGGCAAGG CTTGCAGCAG ACCCAGCAGC AGCAACAGAC GGCCGCCCTG 
GTGCGGCAGC TCCAGAAGCA GCTTTCTAGT AACCAGCCAC AGCAAGGAGT GACTCCCTAT 
GGGCATCCAT CACACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666122.1
CDS	204..6704
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIAAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKELPLAKSGVPQVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQATDPNLEWTQISTRYLREQLAKISDFYHMASSTSDGPVPVPPDVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHMMIGPNNSSIGAPSPGPPGPVMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVSASTRPRSPAGENVDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEAKHQLKKITKDILKILNKKSTTETGVGDEGQKTRKNKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNNVSSLKNDDFTMRGLRRDGNADDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNSGMGLFNPNGIATTDAGSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPMPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLMGQPQQPGFFLQNQSLTPGGSRLDPTGSFVPTNTKQALSNMLQRRSGAMMQPPPLHAITSQQQLIQMKLLQQQQQQRLLRQAQARPFQQDFCNSAVSLLEDFDNIMGPPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGGVVLSPSYNSRTYPATHSNPALMERLRQMQQQPSGYVQQQASPYLQPLTSSQRLNHQSLQQSPLVGGGIDAVLTPTHPSLPSVPLSQDPMRTRQPQVRQQQRLLQMQQPPQPQQPPQPQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_019810563.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810563.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGAGC TGCCTCTGGC CAAGTCAGGT 
GTTCCTCAGG TTCCAATCCT TAGTAAAAAA GAAGATGTTT TTGCATATTT AGCTAAATAT 
TCAGTGCCAA TGGTTCGAGC CACGTGGCTG ATCAAGATGA CGTGTGCCTA TTATTCTGCT 
ATTTCTGAAG CTAAAATTAA GAAACGTCAG GCTACTGATC CTAATTTGGA GTGGACACAA 
ATATCTACCA GATATCTTCG AGAGCAGCTG GCCAAGATTT CTGACTTTTA CCACATGGCT 
TCCAGCACAA GTGATGGTCC TGTCCCAGTG CCACCAGATG TGGAGCAAGC CATGAAGCAA 
TGGGAATACA ACGAAAAGTT GGCATTTCAC ATGTTCCAGG AAGGAATGCT AGAAAAACAT 
GAATATTTGA CATGGATCTT GGATGTGTTA GAGAAGATCA GACCGATGGA TGATGACCTT 
CTTAAACTCT TGTTACCACT CATGCTGCAG TATTCGGACG AGTTTGTTCA GTCGGCTTAC 
CTGTCTCGTC GTCTTGCCTA CTTTTGTGCC CGGCGTCTTG CCTTGCTGCT GAGCGATAGC 
CCCAGCCTCC TTGCTGCCCA CTCGCCCCAC ATGATGATAG GACCAAACAA CTCAAGCATC 
GGGGCCCCCA GCCCTGGCCC CCCTGGCCCC GTCATGAGTC CCGTGCAGCT GGCCTTCTCA 
GACTTCCTCT CCTGTGCACA GCATGGCCCC CTGGTTTATG GACTTAGTTG TATGTTGCAG 
ACTGTCACTC TCTGTTGCCC AAGTGCCTTG GTGTGGAATT ATTCCACAAA CGACAATAAG 
AGCGCGAACC CAGGCTCGCC CCTGGATCTG CTGCAGGTGG CCCCCTCCAG TCTCCCCATG 
CCGGGTGGGA ACACAGCTTT CAATCAGCAG GTTCGGGCAA GGATTTATGA GGTAGAACAG 
CAGATAAAAC AAAGAGGCCG TGCAGTGGAA GTTCGGTGGT CTTTTGACAA GTGCCAAGAG 
TCAACAGCAG GGGTGACCAT TAGTCGGGTT TTGCACACGT TGGAAGTGTT GGATCGACAT 
TGTTTTGACC GAACTGATTC CAGCAATTCA ATGGAGACGC TTTATCATAA GATTTTCTGG 
GCAAACCAAA ACAAAGATAA CCAAGAGGTC GCCCCCAACG ATGAAGCTGT GGTGACCTTG 
CTGTGTGAAT GGGCGGTGAG CTGCAAACGG TCAGGCAAGC ACAGGGCCAT GGCTGTGGCA 
AAACTCTTGG AGAAGAGGCA GGCGGAAATC GAAGCAGAGA GATGTGGTGA ATCAGAGGTC 
TTGGATGAGA AGGAGTCCAT TTCTTCAGCC TCTCTTGCTG GATCTAGTTT GCCTGTTTTC 
CAGAATGTGC TACTAAGGTT TTTAGATACA CAGGCCCCCT CATTATCGGA TCCAAACAGT 
GAATGTGAAA AAGTAGAATT TGTGAATTTG GTGCTGCTCT TCTGTGAGTT CATCCGACAC 
GATGTCTTCT CCCATGATGC GTACATGTGT ACCCTCATAT CTCGGGGAGA CTTGTCAGTC 
AGCGCCTCGA CTCGGCCGCG ATCGCCAGCA GGAGAAAACG TAGATGAGCA CTATCCAAAG 
GACCATGACA TGAAAATGGA GATTTTTTCT CCCATGCCTG GAGAATCCTG TGAGAATGCC 
AACCCTTCCC TGGGCAGAAG AATGTCGGTT AATGGTGAGA AGTTGCTTAA GAGAGAAAAA 
CCCAGGGAGT TGATTTTTCC ATCTAATTAT GACCTTCTGC GGCACCTGCA GTATGCAACA 
CATTTCCCCA TACCTCTGGA TGAATCTTCA AGTCATGAAT GTAACCAGCG CACAATCCTG 
CTTTATGGAG TGGGCAAAGA GCGTGACGAA GCGAAGCATC AGCTGAAGAA GATTACCAAA 
GATATTTTGA AAATCCTAAA TAAGAAGAGC ACCACAGAGA CAGGGGTTGG AGATGAAGGA 
CAAAAAACCA GGAAGAACAA ACAGGAAGCA TTTCCAACTC TGGAGACTGT GTTCACAAAA 
CTTCAGCTCC TTTCATATTT TGATCAGCAT CAAGTGACAT CTCAGATCTC CAATAATGTG 
CTGGAACAAA TCACAAGCTT CGCTTCAGGA ACATCCTATC ATCTCCCTTT GGCTCACCAT 
ATTCAACTCA TCTTTGATCT TATGGAGCCA GCGCTGAACA TCAATGGACT AATTGACTTC 
GCAATACAGC TGCTAAATGA ACTGAGTGTT GTGGAAGCCG AACTGCTCCT GAAATCCTCC 
AGCCTGGCAG GAAGTTACAC GACGGGACTG TGTGTGTGCA TTGTGGCTGT TCTCAGGCGG 
TACCATAGTT GTCTGATCCT GAATCCTGAT CAGACAGCCC AGGTGTTTGA AGGGTTGTGT 
GGTGTGGTAA AGCATGTTGT GAACCCCTCA GAATGTTCTT CCCCCGAGAG ATGCATCTTA 
GCCTACCTCT ACGATCTCTA TGTGTCATGT AGCCACCTCA GAAGTAAATT TGGAGACCTC 
TTCAGTAGTG CCTGTTCAAA AGTTAAGCAA ACCATATATA ATAATGTGAT GCCTGCAAAT 
TCTAACTTGA GATGGGATCC GGACTTCATG ATGGATTTTA TTGAGAATCC TTCAGCTCGT 
AGCATCAATT ACTCAATGCT GGGCAAGATC CTTAGTGACA ATGCAGCCAA TCGCTACAGC 
TTCGTCTGTA ACACGCTCAT GAATGTATGT ATGGGCCATC AGGATGCTGG AAGGATTAAC 
GACATAGCCA ATTTCTCCTC TGAGCTGACA GCTTGCTGCA CTGTTCTTAG TTCAGAATGG 
CTGGGAGTTC TGAAGGCTCT TTGTTGTTCT TCAAATCATG TGTGGGGGTT TAATGATGTA 
CTTTGCACTG TAGATGTGAG TGACCTTTCA TTCCATGATT CATTAGCTAC TTTCATTGCT 
ATTCTGATAG CACGACAGTG TTTCTCCCTG GAGGACGTCG TGCAGCACGT CGCACTTCCC 
TCTCTCCTAG CAGCAGCTTG TGGAGATGCA GATGCTGAGC CGGGGGCGAG AATGACATGC 
CGACTCCTGC TTCATCTCTT CCGAGCTCCC CAGGCCTGCT TGTTACCTCA AGCAACCGGC 
AAACCTTTCC CTGGAATAAG ATCATCTTGT GATAGACACC TCTTAGCCGC CGCTCACAAT 
AGCATTGAAG TGGGAGCCGT GTTTGCTGTC TTAAAAGCAA TTATGATGCT TGGAGATGCC 
AAAATTGGCA ATAACAATGT CAGCTCTTTA AAGAATGATG ACTTCACCAT GAGAGGTTTA 
CGGCGTGATG GGAATGCTGA TGATATCTGG ACTGCTTCAC AAAATTCAAA ATCCTGTGGG 
AAAAGCATTT CCATAGAAAC TGCCAATTTA AGAGAATATG CTAGATATGT ACTGAGGACT 
ATCTGTCAAC AGGAATGGGT AGGAGAACAT TGCCTAAAAG AACCTGAAAG GTTGTGCACA 
GACAAAGAGC TTATATTGGA CCCTGTGCTT TCAAATATGC AAGCACAGAA ATTATTGCAG 
CTTATTTGTT ATCCTCATGG TATTAAAGAA TGTACGGAGG GCGACAATCT GCAGAGACAG 
CACATTAAGC GCATTCTTCA GAATCTTGAG CAGTGGACAC TGAGGCAGTC CTGGCTAGAA 
CTTCAGTTAA TGATCAAACA GTGCTTGAAG GACCCTGGCT CTGGTTCAGT GGCTGAGATG 
AACAACTTAC TGGACAATAT TGCAAAGGCA ACAATAGAGG TATTCCAGCA GTCTGCTGAC 
TTGAACAATA ATTCTTCTAA TTCTGGAATG GGCCTCTTCA ACCCAAATGG GATTGCAACT 
ACTGATGCTG GTAGCACAAG GCAGAATGGA ATAAAGACAT TTCTAAGTTC CTCTGAACGC 
AGGGGTGTAT GGCTGGTGGC CCCCCTAATT GCCAGGCTGC CGACCTCTGT GCAAGGAAGA 
GTATTGAAAG CTGCAGGAGA GGAACTGGAG AAGGGACAGC ACTTGGGCTC TTCTTCAAAA 
AAGGAAAGAG ACAGACAAAA ACAGAAAAGT ATGTCTCTTT TGAGTCAACA ACCTTTCCTC 
TCACTGGTCC TTACCTGCCT TAAGGGACAA GATGAACAAC GGGAAGGCCT CCTAACATCT 
CTTCAGAATC AGGTTAATCA GATTTTAAGT AATTGGAGAG AAGAACGATA CCAGGATGAC 
ATAAAAGCAC GGCAGATGAT GCATGAAGCA TTGCAACTTC GCCTAAATTT GGTAGGAGGA 
ATGTTTGACA CGGTGCAGAG GAGCACCCAG TGGACAACAG ACTGGGCCCT CCTCCTGCTT 
CAGATCATAA CTTCTGGAAC TGTTGACATG CACACTAACA ATGAATTATT CACAACAGTT 
CTTGACATGC TGGGTGTGTT AATCAATGGA ACATTAGCCT CAGACCTATC AAATGCATCC 
CCAGGGGGAT CTGAAGAGAA CAAACGTGCA TACATGAATT TAGTAAAGAA ACTGAAGAAA 
GAGCTAGGAG ACAAGAGATC AGAAAGTATT GACAAAGTTC GACAGTTGCT ACCTTTGCCA 
AAACAGACAT GTGATGTCAT TACTTGTGAA CCTATGGGTT CCTTGATTGA CACAAAGGGA 
AACAAAATTG CTGGATTCGA CTCCATAGAT AAAAAACAGG GTCTTCAGGT GTCTACAAAG 
CAGAAGGTGT CCCCATGGGA TTTGTTTGAG GGTCAGAAGA ACCCAGCTCC TCTGTCTTGG 
GCCTGGTTCG GGACTGTCCG AGTGGACCGA AAGGTGATCA AGTACGAGGA GCAGCATCAC 
CTGCTGTTGT ATCACACACA CCCCATGCCC AAGCCCCGAA GTTACTACCT CGAACCGCTG 
CCCCTGCCTC CCGAGGAGGA AGAGGAGGAG CCCACATCTC CCATCTCTCA GGAACCAGAG 
CGGAAATCAG CTGAGCTGTC AGATCAGGGA AAAACCGCAA CAGATGAAGA GAAGAAAACA 
AAAGGAAGGA AGCGCAAGAC GAAATCAAGC TCAAGAGTTG ACGAGTATCC ACAGAGCAAC 
ATATACCGAG TGCCTCCTAA CTACTCACCC ATTTCCTCCC AGATGATGCA CCATCCGCAG 
TCCACCTTAT GGGGCTATAA CCTCATGGGT CAGCCCCAGC AGCCGGGTTT TTTCCTTCAG 
AACCAATCTC TTACTCCAGG TGGTTCTAGA TTGGACCCCA CGGGCTCCTT CGTCCCAACG 
AATACCAAAC AAGCTCTGTC CAACATGCTG CAGCGGCGCT CGGGGGCCAT GATGCAGCCG 
CCGCCCCTCC ACGCCATCAC ATCGCAGCAG CAGCTGATAC AGATGAAGCT TCTGCAGCAG 
CAGCAGCAGC AGCGGCTTCT CCGGCAAGCC CAGGCTCGAC CTTTCCAGCA GGACTTCTGT 
AACTCTGCTG TCTCCCTTCT GGAAGACTTT GACAACATAA TGGGCCCGCC AGGGGACCAG 
GCTGCTCTCT TTGCTGCACA AGCACGGCCC TCCCCTCAGC TCCCCCAGTA TCCAGGGCTG 
CAGCAAGCAC AGACCATGCC CCAGGGCTAT ACGATGTATG GAACGCAGAT GCCTTTGCAG 
CAGACCCCTC AGCAGCAGGC TGGTGGCGTA GTCCTGTCTC CTAGCTATAA CTCCAGGACC 
TATCCGGCCA CACATTCCAA CCCGGCGCTA ATGGAAAGAC TCAGACAGAT GCAGCAGCAG 
CCCAGTGGCT ATGTTCAGCA GCAGGCGTCA CCGTACCTGC AGCCCTTGAC CAGCTCTCAG 
AGACTAAATC ATCAGTCGCT TCAGCAGAGC CCTCTGGTAG GTGGAGGAAT CGATGCAGTG 
CTCACTCCCA CGCATCCAAG CCTCCCCTCG GTGCCCCTGT CTCAGGACCC AATGAGAACC 
AGACAGCCGC AGGTTCGACA GCAGCAGAGA CTCCTCCAGA TGCAGCAGCC CCCCCAGCCC 
CAGCAGCCCC CGCAGCCCCA GAGTCAGACC CTTGGTCTCC AAGCGATGCA GCCCCAGCAG 
CCTTTGTTTC CCAGGCAAGG CTTGCAGCAG ACCCAGCAGC AGCAACAGAC GGCCGCCCTG 
GTGCGGCAGC TCCAGAAGCA GCTTTCTAGT AACCAGCCAC AGCAAGGAGT GACTCCCTAT 
GGGCATCCAT CACACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_019810564.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019666123.1 Felis catus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

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CloneID	OFb48840
Clone ID Related Accession (Same CDS sequence)	XM_019810564.1
Accession Version	XM_019810564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	mediator of RNA polymerase II transcription subunit 12-like protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC CCTTCCCTGG GCAGAAGAAT GTCGGTTAAT 
GGTGAGAAGT TGCTTAAGAG AGAAAAACCC AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTTCTGCGGC ACCTGCAGTA TGCAACACAT TTCCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTGCTT TATGGAGTGG GCAAAGAGCG TGACGAAGCG 
AAGCATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAA AAAACCAGGA AGAACAAACA GGAAGCATTT 
CCAACTCTGG AGACTGTGTT CACAAAACTT CAGCTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATCTCCAA TAATGTGCTG GAACAAATCA CAAGCTTCGC TTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCATATT CAACTCATCT TTGATCTTAT GGAGCCAGCG 
CTGAACATCA ATGGACTAAT TGACTTCGCA ATACAGCTGC TAAATGAACT GAGTGTTGTG 
GAAGCCGAAC TGCTCCTGAA ATCCTCCAGC CTGGCAGGAA GTTACACGAC GGGACTGTGT 
GTGTGCATTG TGGCTGTTCT CAGGCGGTAC CATAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTAAAGC ATGTTGTGAA CCCCTCAGAA 
TGTTCTTCCC CCGAGAGATG CATCTTAGCC TACCTCTACG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT TAAGCAAACC 
ATATATAATA ATGTGATGCC TGCAAATTCT AACTTGAGAT GGGATCCGGA CTTCATGATG 
GATTTTATTG AGAATCCTTC AGCTCGTAGC ATCAATTACT CAATGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGTAACA CGCTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGAAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTGACAGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGAGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AGCACGTCGC ACTTCCCTCT CTCCTAGCAG CAGCTTGTGG AGATGCAGAT 
GCTGAGCCGG GGGCGAGAAT GACATGCCGA CTCCTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTGT TACCTCAAGC AACCGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCCGC TCACAATAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAATGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTACGG CGTGATGGGA ATGCTGATGA TATCTGGACT 
GCTTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAACATTGC 
CTAAAAGAAC CTGAAAGGTT GTGCACAGAC AAAGAGCTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ATTGCAGCTT ATTTGTTATC CTCATGGTAT TAAAGAATGT 
ACGGAGGGCG ACAATCTGCA GAGACAGCAC ATTAAGCGCA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAGTCCTG GCTAGAACTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCAGTGGC TGAGATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCTGACTTG AACAATAATT CTTCTAATTC TGGAATGGGC 
CTCTTCAACC CAAATGGGAT TGCAACTACT GATGCTGGTA GCACAAGGCA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTATGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CCTCTGTGCA AGGAAGAGTA TTGAAAGCTG CAGGAGAGGA ACTGGAGAAG 
GGACAGCACT TGGGCTCTTC TTCAAAAAAG GAAAGAGACA GACAAAAACA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC TTTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGGG AAGGCCTCCT AACATCTCTT CAGAATCAGG TTAATCAGAT TTTAAGTAAT 
TGGAGAGAAG AACGATACCA GGATGACATA AAAGCACGGC AGATGATGCA TGAAGCATTG 
CAACTTCGCC TAAATTTGGT AGGAGGAATG TTTGACACGG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCCCTCCT CCTGCTTCAG ATCATAACTT CTGGAACTGT TGACATGCAC 
ACTAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTGTTAAT CAATGGAACA 
TTAGCCTCAG ACCTATCAAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAATTTAG TAAAGAAACT GAAGAAAGAG CTAGGAGACA AGAGATCAGA AAGTATTGAC 
AAAGTTCGAC AGTTGCTACC TTTGCCAAAA CAGACATGTG ATGTCATTAC TTGTGAACCT 
ATGGGTTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGACTC CATAGATAAA 
AAACAGGGTC TTCAGGTGTC TACAAAGCAG AAGGTGTCCC CATGGGATTT GTTTGAGGGT 
CAGAAGAACC CAGCTCCTCT GTCTTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGAAAG 
GTGATCAAGT ACGAGGAGCA GCATCACCTG CTGTTGTATC ACACACACCC CATGCCCAAG 
CCCCGAAGTT ACTACCTCGA ACCGCTGCCC CTGCCTCCCG AGGAGGAAGA GGAGGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCAGAGCGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAAACAAAA GGAAGGAAGC GCAAGACGAA ATCAAGCTCA 
AGAGTTGACG AGTATCCACA GAGCAACATA TACCGAGTGC CTCCTAACTA CTCACCCATT 
TCCTCCCAGA TGATGCACCA TCCGCAGTCC ACCTTATGGG GCTATAACCT CATGGGTCAG 
CCCCAGCAGC CGGGTTTTTT CCTTCAGAAC CAATCTCTTA CTCCAGGTGG TTCTAGATTG 
GACCCCACGG GCTCCTTCGT CCCAACGAAT ACCAAACAAG CTCTGTCCAA CATGCTGCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCG CCCCTCCACG CCATCACATC GCAGCAGCAG 
CTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAGCAGC GGCTTCTCCG GCAAGCCCAG 
GCTCGACCTT TCCAGCAGGA CTTCTGTAAC TCTGCTGTCT CCCTTCTGGA AGACTTTGAC 
AACATAATGG GCCCGCCAGG GGACCAGGCT GCTCTCTTTG CTGCACAAGC ACGGCCCTCC 
CCTCAGCTCC CCCAGTATCC AGGGCTGCAG CAAGCACAGA CCATGCCCCA GGGCTATACG 
ATGTATGGAA CGCAGATGCC TTTGCAGCAG ACCCCTCAGC AGCAGGCTGG TGGCGTAGTC 
CTGTCTCCTA GCTATAACTC CAGGACCTAT CCGGCCACAC ATTCCAACCC GGCGCTAATG 
GAAAGACTCA GACAGATGCA GCAGCAGCCC AGTGGCTATG TTCAGCAGCA GGCGTCACCG 
TACCTGCAGC CCTTGACCAG CTCTCAGAGA CTAAATCATC AGTCGCTTCA GCAGAGCCCT 
CTGGTAGGTG GAGGAATCGA TGCAGTGCTC ACTCCCACGC ATCCAAGCCT CCCCTCGGTG 
CCCCTGTCTC AGGACCCAAT GAGAACCAGA CAGCCGCAGG TTCGACAGCA GCAGAGACTC 
CTCCAGATGC AGCAGCCCCC CCAGCCCCAG CAGCCCCCGC AGCCCCAGAG TCAGACCCTT 
GGTCTCCAAG CGATGCAGCC CCAGCAGCCT TTGTTTCCCA GGCAAGGCTT GCAGCAGACC 
CAGCAGCAGC AACAGACGGC CGCCCTGGTG CGGCAGCTCC AGAAGCAGCT TTCTAGTAAC 
CAGCCACAGC AAGGAGTGAC TCCCTATGGG CATCCATCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666123.1
CDS	204..6671
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIAAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQATDPNLEWTQISTRYLREQLAKISDFYHMASSTSDGPVPVPPDVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHMMIGPNNSSIGAPSPGPPGPVMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVSASTRPRSPAGENVDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEAKHQLKKITKDILKILNKKSTTETGVGDEGQKTRKNKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNNVSSLKNDDFTMRGLRRDGNADDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNSGMGLFNPNGIATTDAGSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPMPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLMGQPQQPGFFLQNQSLTPGGSRLDPTGSFVPTNTKQALSNMLQRRSGAMMQPPPLHAITSQQQLIQMKLLQQQQQQRLLRQAQARPFQQDFCNSAVSLLEDFDNIMGPPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGGVVLSPSYNSRTYPATHSNPALMERLRQMQQQPSGYVQQQASPYLQPLTSSQRLNHQSLQQSPLVGGGIDAVLTPTHPSLPSVPLSQDPMRTRQPQVRQQQRLLQMQQPPQPQQPPQPQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_019810564.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus mediator complex subunit 12 like (MED12L), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810564.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC CCTTCCCTGG GCAGAAGAAT GTCGGTTAAT 
GGTGAGAAGT TGCTTAAGAG AGAAAAACCC AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTTCTGCGGC ACCTGCAGTA TGCAACACAT TTCCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTGCTT TATGGAGTGG GCAAAGAGCG TGACGAAGCG 
AAGCATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAA AAAACCAGGA AGAACAAACA GGAAGCATTT 
CCAACTCTGG AGACTGTGTT CACAAAACTT CAGCTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATCTCCAA TAATGTGCTG GAACAAATCA CAAGCTTCGC TTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCATATT CAACTCATCT TTGATCTTAT GGAGCCAGCG 
CTGAACATCA ATGGACTAAT TGACTTCGCA ATACAGCTGC TAAATGAACT GAGTGTTGTG 
GAAGCCGAAC TGCTCCTGAA ATCCTCCAGC CTGGCAGGAA GTTACACGAC GGGACTGTGT 
GTGTGCATTG TGGCTGTTCT CAGGCGGTAC CATAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTAAAGC ATGTTGTGAA CCCCTCAGAA 
TGTTCTTCCC CCGAGAGATG CATCTTAGCC TACCTCTACG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT TAAGCAAACC 
ATATATAATA ATGTGATGCC TGCAAATTCT AACTTGAGAT GGGATCCGGA CTTCATGATG 
GATTTTATTG AGAATCCTTC AGCTCGTAGC ATCAATTACT CAATGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGTAACA CGCTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGAAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTGACAGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGAGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AGCACGTCGC ACTTCCCTCT CTCCTAGCAG CAGCTTGTGG AGATGCAGAT 
GCTGAGCCGG GGGCGAGAAT GACATGCCGA CTCCTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTGT TACCTCAAGC AACCGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCCGC TCACAATAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAATGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTACGG CGTGATGGGA ATGCTGATGA TATCTGGACT 
GCTTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAACATTGC 
CTAAAAGAAC CTGAAAGGTT GTGCACAGAC AAAGAGCTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ATTGCAGCTT ATTTGTTATC CTCATGGTAT TAAAGAATGT 
ACGGAGGGCG ACAATCTGCA GAGACAGCAC ATTAAGCGCA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAGTCCTG GCTAGAACTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCAGTGGC TGAGATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCTGACTTG AACAATAATT CTTCTAATTC TGGAATGGGC 
CTCTTCAACC CAAATGGGAT TGCAACTACT GATGCTGGTA GCACAAGGCA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTATGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CCTCTGTGCA AGGAAGAGTA TTGAAAGCTG CAGGAGAGGA ACTGGAGAAG 
GGACAGCACT TGGGCTCTTC TTCAAAAAAG GAAAGAGACA GACAAAAACA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC TTTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGGG AAGGCCTCCT AACATCTCTT CAGAATCAGG TTAATCAGAT TTTAAGTAAT 
TGGAGAGAAG AACGATACCA GGATGACATA AAAGCACGGC AGATGATGCA TGAAGCATTG 
CAACTTCGCC TAAATTTGGT AGGAGGAATG TTTGACACGG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCCCTCCT CCTGCTTCAG ATCATAACTT CTGGAACTGT TGACATGCAC 
ACTAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTGTTAAT CAATGGAACA 
TTAGCCTCAG ACCTATCAAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAATTTAG TAAAGAAACT GAAGAAAGAG CTAGGAGACA AGAGATCAGA AAGTATTGAC 
AAAGTTCGAC AGTTGCTACC TTTGCCAAAA CAGACATGTG ATGTCATTAC TTGTGAACCT 
ATGGGTTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGACTC CATAGATAAA 
AAACAGGGTC TTCAGGTGTC TACAAAGCAG AAGGTGTCCC CATGGGATTT GTTTGAGGGT 
CAGAAGAACC CAGCTCCTCT GTCTTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGAAAG 
GTGATCAAGT ACGAGGAGCA GCATCACCTG CTGTTGTATC ACACACACCC CATGCCCAAG 
CCCCGAAGTT ACTACCTCGA ACCGCTGCCC CTGCCTCCCG AGGAGGAAGA GGAGGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCAGAGCGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAAACAAAA GGAAGGAAGC GCAAGACGAA ATCAAGCTCA 
AGAGTTGACG AGTATCCACA GAGCAACATA TACCGAGTGC CTCCTAACTA CTCACCCATT 
TCCTCCCAGA TGATGCACCA TCCGCAGTCC ACCTTATGGG GCTATAACCT CATGGGTCAG 
CCCCAGCAGC CGGGTTTTTT CCTTCAGAAC CAATCTCTTA CTCCAGGTGG TTCTAGATTG 
GACCCCACGG GCTCCTTCGT CCCAACGAAT ACCAAACAAG CTCTGTCCAA CATGCTGCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCG CCCCTCCACG CCATCACATC GCAGCAGCAG 
CTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAGCAGC GGCTTCTCCG GCAAGCCCAG 
GCTCGACCTT TCCAGCAGGA CTTCTGTAAC TCTGCTGTCT CCCTTCTGGA AGACTTTGAC 
AACATAATGG GCCCGCCAGG GGACCAGGCT GCTCTCTTTG CTGCACAAGC ACGGCCCTCC 
CCTCAGCTCC CCCAGTATCC AGGGCTGCAG CAAGCACAGA CCATGCCCCA GGGCTATACG 
ATGTATGGAA CGCAGATGCC TTTGCAGCAG ACCCCTCAGC AGCAGGCTGG TGGCGTAGTC 
CTGTCTCCTA GCTATAACTC CAGGACCTAT CCGGCCACAC ATTCCAACCC GGCGCTAATG 
GAAAGACTCA GACAGATGCA GCAGCAGCCC AGTGGCTATG TTCAGCAGCA GGCGTCACCG 
TACCTGCAGC CCTTGACCAG CTCTCAGAGA CTAAATCATC AGTCGCTTCA GCAGAGCCCT 
CTGGTAGGTG GAGGAATCGA TGCAGTGCTC ACTCCCACGC ATCCAAGCCT CCCCTCGGTG 
CCCCTGTCTC AGGACCCAAT GAGAACCAGA CAGCCGCAGG TTCGACAGCA GCAGAGACTC 
CTCCAGATGC AGCAGCCCCC CCAGCCCCAG CAGCCCCCGC AGCCCCAGAG TCAGACCCTT 
GGTCTCCAAG CGATGCAGCC CCAGCAGCCT TTGTTTCCCA GGCAAGGCTT GCAGCAGACC 
CAGCAGCAGC AACAGACGGC CGCCCTGGTG CGGCAGCTCC AGAAGCAGCT TTCTAGTAAC 
CAGCCACAGC AAGGAGTGAC TCCCTATGGG CATCCATCAC ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12L ( XM_019810565.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019666124.1 Felis catus mediator complex subunit 12 like (MED12L), mRNA.

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CloneID	OFb48841
Clone ID Related Accession (Same CDS sequence)	XM_019810565.1
Accession Version	XM_019810565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	LOW QUALITY PROTEIN: mediator of RNA polymerase II transcription subunit 12-like protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC CCTTCCCTGG GCAGAAGAAT GTCGGTTAAT 
GGTGAGAAGT TGCTTAAGAG AGAAAAACCC AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTTCTGCGGC ACCTGCAGTA TGCAACACAT TTCCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTGCTT TATGGAGTGG GCAAAGAGCG TGACGAAGCG 
AAGCATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAA AAAACCAGGA AGAACAAACA GGAAGCATTT 
CCAACTCTGG AGACTGTGTT CACAAAACTT CAGCTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATCTCCAA TAATGTGCTG GAACAAATCA CAAGCTTCGC TTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCATATT CAACTCATCT TTGATCTTAT GGAGCCAGCG 
CTGAACATCA ATGGACTAAT TGACTTCGCA ATACAGCTGC TAAATGAACT GAGTGTTGTG 
GAAGCCGAAC TGCTCCTGAA ATCCTCCAGC CTGGCAGGAA GTTACACGAC GGGACTGTGT 
GTGTGCATTG TGGCTGTTCT CAGGCGGTAC CATAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTAAAGC ATGTTGTGAA CCCCTCAGAA 
TGTTCTTCCC CCGAGAGATG CATCTTAGCC TACCTCTACG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT TAAGCAAACC 
ATATATAATA ATGTGATGCC TGCAAATTCT AACTTGAGAT GGGATCCGGA CTTCATGATG 
GATTTTATTG AGAATCCTTC AGCTCGTAGC ATCAATTACT CAATGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGTAACA CGCTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGAAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTGACAGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGAGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AGCACGTCGC ACTTCCCTCT CTCCTAGCAG CAGCTTGTGG AGATGCAGAT 
GCTGAGCCGG GGGCGAGAAT GACATGCCGA CTCCTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTGT TACCTCAAGC AACCGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCCGC TCACAATAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAATGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTACGG CGTGATGGGA ATGCTGATGA TATCTGGACT 
GCTTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAACATTGC 
CTAAAAGAAC CTGAAAGGTT GTGCACAGAC AAAGAGCTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ATTGCAGCTT ATTTGTTATC CTCATGGTAT TAAAGAATGT 
ACGGAGGGCG ACAATCTGCA GAGACAGCAC ATTAAGCGCA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAGTCCTG GCTAGAACTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCAGTGGC TGAGATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCTGACTTG AACAATAATT CTTCTAATTC TGGAATGGGC 
CTCTTCAACC CAAATGGGAT TGCAACTACT GATGCTGGTA GCACAAGGCA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTATGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CCTCTGTGCA AGGAAGAGTA TTGAAAGCTG CAGGAGAGGA ACTGGAGAAG 
GGACAGCACT TGGGCTCTTC TTCAAAAAAG GAAAGAGACA GACAAAAACA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC TTTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGGG AAGGCCTCCT AACATCTCTT CAGAATCAGG TTAATCAGAT TTTAAGTAAT 
TGGAGAGAAG AACGATACCA GGATGACATA AAAGCACGGC AGATGATGCA TGAAGCATTG 
CAACTTCGCC TAAATTTGGT AGGAGGAATG TTTGACACGG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCCCTCCT CCTGCTTCAG ATCATAACTT CTGGAACTGT TGACATGCAC 
ACTAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTGTTAAT CAATGGAACA 
TTAGCCTCAG ACCTATCAAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAATTTAG TAAAGAAACT GAAGAAAGAG CTAGGAGACA AGAGATCAGA AAGTATTGAC 
AAAGTTCGAC AGTTGCTACC TTTGCCAAAA CAGACATGTG ATGTCATTAC TTGTGAACCT 
ATGGGTTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGACTC CATAGATAAA 
AAACAGGGTC TTCAGGTGTC TACAAAGCAG AAGGTGTCCC CATGGGATTT GTTTGAGGGT 
CAGAAGAACC CAGCTCCTCT GTCTTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGAAAG 
GTGATCAAGT ACGAGGAGCA GCATCACCTG CTGTTGTATC ACACACACCC CATGCCCAAG 
CCCCGAAGTT ACTACCTCGA ACCGCTGCCC CTGCCTCCCG AGGAGGAAGA GGAGGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCAGAGCGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAAACAAAA GGAAGGAAGC GCAAGACGAA ATCAAGCTCA 
AGAGTTGACG AGTATCCACA GAGCAACATA TACCGAGTGC CTCCTAACTA CTCACCCATT 
TCCTCCCAGA TGATGCACCA TCCGCAGTCC ACCTTATGGG GCTATAACCT CATGGGTCAG 
CCCCAGCAGC CGGGTTTTTT CCTTCAGAAC CAATCTCTTA CTCCAGGTGG TTCTAGATTG 
GACCCCACGG GCTCCTTCGT CCCAACGAAT ACCAAACAAG CTCTGTCCAA CATGCTGCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCG CCCCTCCACG CCATCACATC GCAGCAGCAG 
CTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAGCAGC GGCTTCTCCG GCAAGCCCAG 
GCTCGACCTT TCCAGCAGGG CCCGCCAGGG GACCAGGCTG CTCTCTTTGC TGCACAAGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGGCTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTATACGA TGTATGGAAC GCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGT 
GGCGTAGTCC TGTCTCCTAG CTATAACTCC AGGACCTATC CGGCCACACA TTCCAACCCG 
GCGCTAATGG AAAGACTCAG ACAGATGCAG CAGCAGCCCA GTGGCTATGT TCAGCAGCAG 
GCGTCACCGT ACCTGCAGCC CTTGACCAGC TCTCAGAGAC TAAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTAGGTGG AGGAATCGAT GCAGTGCTCA CTCCCACGCA TCCAAGCCTC 
CCCTCGGTGC CCCTGTCTCA GGACCCAATG AGAACCAGAC AGCCGCAGGT TCGACAGCAG 
CAGAGACTCC TCCAGATGCA GCAGCCCCCC CAGCCCCAGC AGCCCCCGCA GCCCCAGAGT 
CAGACCCTTG GTCTCCAAGC GATGCAGCCC CAGCAGCCTT TGTTTCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAGCA ACAGACGGCC GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCTAGTAACC AGCCACAGCA AGGAGTGACT CCCTATGGGC ATCCATCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019666124.1
CDS	1..6417
Translation	MAAFGLLSYEQRPLKRPRLGPPDVYPQDPKQKEDELTAVNVKQGFNNQPAFTGDEHGSARNIVINPSKIAAYFSSILAEKLKLNTFQDTGKKKPQVNAKDNYWLVTARSQSAIHSWFSDLAGNKPLAILAKKVPILSKKEDVFAYLAKYSVPMVRATWLIKMTCAYYSAISEAKIKKRQATDPNLEWTQISTRYLREQLAKISDFYHMASSTSDGPVPVPPDVEQAMKQWEYNEKLAFHMFQEGMLEKHEYLTWILDVLEKIRPMDDDLLKLLLPLMLQYSDEFVQSAYLSRRLAYFCARRLALLLSDSPSLLAAHSPHMMIGPNNSSIGAPSPGPPGPVMSPVQLAFSDFLSCAQHGPLVYGLSCMLQTVTLCCPSALVWNYSTNDNKSANPGSPLDLLQVAPSSLPMPGGNTAFNQQVRARIYEVEQQIKQRGRAVEVRWSFDKCQESTAGVTISRVLHTLEVLDRHCFDRTDSSNSMETLYHKIFWANQNKDNQEVAPNDEAVVTLLCEWAVSCKRSGKHRAMAVAKLLEKRQAEIEAERCGESEVLDEKESISSASLAGSSLPVFQNVLLRFLDTQAPSLSDPNSECEKVEFVNLVLLFCEFIRHDVFSHDAYMCTLISRGDLSVSASTRPRSPAGENVDEHYPKDHDMKMEIFSPMPGESCENANPSLGRRMSVNGEKLLKREKPRELIFPSNYDLLRHLQYATHFPIPLDESSSHECNQRTILLYGVGKERDEAKHQLKKITKDILKILNKKSTTETGVGDEGQKTRKNKQEAFPTLETVFTKLQLLSYFDQHQVTSQISNNVLEQITSFASGTSYHLPLAHHIQLIFDLMEPALNINGLIDFAIQLLNELSVVEAELLLKSSSLAGSYTTGLCVCIVAVLRRYHSCLILNPDQTAQVFEGLCGVVKHVVNPSECSSPERCILAYLYDLYVSCSHLRSKFGDLFSSACSKVKQTIYNNVMPANSNLRWDPDFMMDFIENPSARSINYSMLGKILSDNAANRYSFVCNTLMNVCMGHQDAGRINDIANFSSELTACCTVLSSEWLGVLKALCCSSNHVWGFNDVLCTVDVSDLSFHDSLATFIAILIARQCFSLEDVVQHVALPSLLAAACGDADAEPGARMTCRLLLHLFRAPQACLLPQATGKPFPGIRSSCDRHLLAAAHNSIEVGAVFAVLKAIMMLGDAKIGNNNVSSLKNDDFTMRGLRRDGNADDIWTASQNSKSCGKSISIETANLREYARYVLRTICQQEWVGEHCLKEPERLCTDKELILDPVLSNMQAQKLLQLICYPHGIKECTEGDNLQRQHIKRILQNLEQWTLRQSWLELQLMIKQCLKDPGSGSVAEMNNLLDNIAKATIEVFQQSADLNNNSSNSGMGLFNPNGIATTDAGSTRQNGIKTFLSSSERRGVWLVAPLIARLPTSVQGRVLKAAGEELEKGQHLGSSSKKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLQNQVNQILSNWREERYQDDIKARQMMHEALQLRLNLVGGMFDTVQRSTQWTTDWALLLLQIITSGTVDMHTNNELFTTVLDMLGVLINGTLASDLSNASPGGSEENKRAYMNLVKKLKKELGDKRSESIDKVRQLLPLPKQTCDVITCEPMGSLIDTKGNKIAGFDSIDKKQGLQVSTKQKVSPWDLFEGQKNPAPLSWAWFGTVRVDRKVIKYEEQHHLLLYHTHPMPKPRSYYLEPLPLPPEEEEEEPTSPISQEPERKSAELSDQGKTATDEEKKTKGRKRKTKSSSRVDEYPQSNIYRVPPNYSPISSQMMHHPQSTLWGYNLMGQPQQPGFFLQNQSLTPGGSRLDPTGSFVPTNTKQALSNMLQRRSGAMMQPPPLHAITSQQQLIQMKLLQQQQQQRLLRQAQARPFQQGPPGDQAALFAAQARPSPQLPQYPGLQQAQTMPQGYTMYGTQMPLQQTPQQQAGGVVLSPSYNSRTYPATHSNPALMERLRQMQQQPSGYVQQQASPYLQPLTSSQRLNHQSLQQSPLVGGGIDAVLTPTHPSLPSVPLSQDPMRTRQPQVRQQQRLLQMQQPPQPQQPPQPQSQTLGLQAMQPQQPLFPRQGLQQTQQQQQTAALVRQLQKQLSSNQPQQGVTPYGHPSHF

Target ORF information:

RefSeq Version	XM_019810565.1
Organism	Felis catus(domestic cat)
Definition	Felis catus mediator complex subunit 12 like (MED12L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019810565.1

ORF Insert Sequence:

ATGGCCGCCT TCGGGCTTCT CAGCTATGAG CAGAGACCGC TGAAACGCCC CCGGCTCGGG 
CCGCCCGACG TCTACCCGCA GGACCCCAAG CAGAAGGAGG ATGAACTTAC TGCTGTGAAT 
GTGAAACAAG GCTTCAACAA TCAGCCAGCC TTTACTGGAG ATGAACATGG CTCAGCCAGA 
AATATTGTAA TTAACCCATC AAAGATTGCA GCTTATTTCA GCAGCATATT AGCTGAGAAA 
CTAAAGCTTA ACACTTTCCA GGACACCGGA AAGAAGAAAC CACAAGTTAA TGCTAAAGAT 
AATTACTGGC TGGTTACTGC TCGATCCCAG AGTGCAATTC ATAGTTGGTT CTCTGACTTA 
GCAGGAAATA AACCACTTGC TATTTTGGCA AAAAAGGTTC CAATCCTTAG TAAAAAAGAA 
GATGTTTTTG CATATTTAGC TAAATATTCA GTGCCAATGG TTCGAGCCAC GTGGCTGATC 
AAGATGACGT GTGCCTATTA TTCTGCTATT TCTGAAGCTA AAATTAAGAA ACGTCAGGCT 
ACTGATCCTA ATTTGGAGTG GACACAAATA TCTACCAGAT ATCTTCGAGA GCAGCTGGCC 
AAGATTTCTG ACTTTTACCA CATGGCTTCC AGCACAAGTG ATGGTCCTGT CCCAGTGCCA 
CCAGATGTGG AGCAAGCCAT GAAGCAATGG GAATACAACG AAAAGTTGGC ATTTCACATG 
TTCCAGGAAG GAATGCTAGA AAAACATGAA TATTTGACAT GGATCTTGGA TGTGTTAGAG 
AAGATCAGAC CGATGGATGA TGACCTTCTT AAACTCTTGT TACCACTCAT GCTGCAGTAT 
TCGGACGAGT TTGTTCAGTC GGCTTACCTG TCTCGTCGTC TTGCCTACTT TTGTGCCCGG 
CGTCTTGCCT TGCTGCTGAG CGATAGCCCC AGCCTCCTTG CTGCCCACTC GCCCCACATG 
ATGATAGGAC CAAACAACTC AAGCATCGGG GCCCCCAGCC CTGGCCCCCC TGGCCCCGTC 
ATGAGTCCCG TGCAGCTGGC CTTCTCAGAC TTCCTCTCCT GTGCACAGCA TGGCCCCCTG 
GTTTATGGAC TTAGTTGTAT GTTGCAGACT GTCACTCTCT GTTGCCCAAG TGCCTTGGTG 
TGGAATTATT CCACAAACGA CAATAAGAGC GCGAACCCAG GCTCGCCCCT GGATCTGCTG 
CAGGTGGCCC CCTCCAGTCT CCCCATGCCG GGTGGGAACA CAGCTTTCAA TCAGCAGGTT 
CGGGCAAGGA TTTATGAGGT AGAACAGCAG ATAAAACAAA GAGGCCGTGC AGTGGAAGTT 
CGGTGGTCTT TTGACAAGTG CCAAGAGTCA ACAGCAGGGG TGACCATTAG TCGGGTTTTG 
CACACGTTGG AAGTGTTGGA TCGACATTGT TTTGACCGAA CTGATTCCAG CAATTCAATG 
GAGACGCTTT ATCATAAGAT TTTCTGGGCA AACCAAAACA AAGATAACCA AGAGGTCGCC 
CCCAACGATG AAGCTGTGGT GACCTTGCTG TGTGAATGGG CGGTGAGCTG CAAACGGTCA 
GGCAAGCACA GGGCCATGGC TGTGGCAAAA CTCTTGGAGA AGAGGCAGGC GGAAATCGAA 
GCAGAGAGAT GTGGTGAATC AGAGGTCTTG GATGAGAAGG AGTCCATTTC TTCAGCCTCT 
CTTGCTGGAT CTAGTTTGCC TGTTTTCCAG AATGTGCTAC TAAGGTTTTT AGATACACAG 
GCCCCCTCAT TATCGGATCC AAACAGTGAA TGTGAAAAAG TAGAATTTGT GAATTTGGTG 
CTGCTCTTCT GTGAGTTCAT CCGACACGAT GTCTTCTCCC ATGATGCGTA CATGTGTACC 
CTCATATCTC GGGGAGACTT GTCAGTCAGC GCCTCGACTC GGCCGCGATC GCCAGCAGGA 
GAAAACGTAG ATGAGCACTA TCCAAAGGAC CATGACATGA AAATGGAGAT TTTTTCTCCC 
ATGCCTGGAG AATCCTGTGA GAATGCCAAC CCTTCCCTGG GCAGAAGAAT GTCGGTTAAT 
GGTGAGAAGT TGCTTAAGAG AGAAAAACCC AGGGAGTTGA TTTTTCCATC TAATTATGAC 
CTTCTGCGGC ACCTGCAGTA TGCAACACAT TTCCCCATAC CTCTGGATGA ATCTTCAAGT 
CATGAATGTA ACCAGCGCAC AATCCTGCTT TATGGAGTGG GCAAAGAGCG TGACGAAGCG 
AAGCATCAGC TGAAGAAGAT TACCAAAGAT ATTTTGAAAA TCCTAAATAA GAAGAGCACC 
ACAGAGACAG GGGTTGGAGA TGAAGGACAA AAAACCAGGA AGAACAAACA GGAAGCATTT 
CCAACTCTGG AGACTGTGTT CACAAAACTT CAGCTCCTTT CATATTTTGA TCAGCATCAA 
GTGACATCTC AGATCTCCAA TAATGTGCTG GAACAAATCA CAAGCTTCGC TTCAGGAACA 
TCCTATCATC TCCCTTTGGC TCACCATATT CAACTCATCT TTGATCTTAT GGAGCCAGCG 
CTGAACATCA ATGGACTAAT TGACTTCGCA ATACAGCTGC TAAATGAACT GAGTGTTGTG 
GAAGCCGAAC TGCTCCTGAA ATCCTCCAGC CTGGCAGGAA GTTACACGAC GGGACTGTGT 
GTGTGCATTG TGGCTGTTCT CAGGCGGTAC CATAGTTGTC TGATCCTGAA TCCTGATCAG 
ACAGCCCAGG TGTTTGAAGG GTTGTGTGGT GTGGTAAAGC ATGTTGTGAA CCCCTCAGAA 
TGTTCTTCCC CCGAGAGATG CATCTTAGCC TACCTCTACG ATCTCTATGT GTCATGTAGC 
CACCTCAGAA GTAAATTTGG AGACCTCTTC AGTAGTGCCT GTTCAAAAGT TAAGCAAACC 
ATATATAATA ATGTGATGCC TGCAAATTCT AACTTGAGAT GGGATCCGGA CTTCATGATG 
GATTTTATTG AGAATCCTTC AGCTCGTAGC ATCAATTACT CAATGCTGGG CAAGATCCTT 
AGTGACAATG CAGCCAATCG CTACAGCTTC GTCTGTAACA CGCTCATGAA TGTATGTATG 
GGCCATCAGG ATGCTGGAAG GATTAACGAC ATAGCCAATT TCTCCTCTGA GCTGACAGCT 
TGCTGCACTG TTCTTAGTTC AGAATGGCTG GGAGTTCTGA AGGCTCTTTG TTGTTCTTCA 
AATCATGTGT GGGGGTTTAA TGATGTACTT TGCACTGTAG ATGTGAGTGA CCTTTCATTC 
CATGATTCAT TAGCTACTTT CATTGCTATT CTGATAGCAC GACAGTGTTT CTCCCTGGAG 
GACGTCGTGC AGCACGTCGC ACTTCCCTCT CTCCTAGCAG CAGCTTGTGG AGATGCAGAT 
GCTGAGCCGG GGGCGAGAAT GACATGCCGA CTCCTGCTTC ATCTCTTCCG AGCTCCCCAG 
GCCTGCTTGT TACCTCAAGC AACCGGCAAA CCTTTCCCTG GAATAAGATC ATCTTGTGAT 
AGACACCTCT TAGCCGCCGC TCACAATAGC ATTGAAGTGG GAGCCGTGTT TGCTGTCTTA 
AAAGCAATTA TGATGCTTGG AGATGCCAAA ATTGGCAATA ACAATGTCAG CTCTTTAAAG 
AATGATGACT TCACCATGAG AGGTTTACGG CGTGATGGGA ATGCTGATGA TATCTGGACT 
GCTTCACAAA ATTCAAAATC CTGTGGGAAA AGCATTTCCA TAGAAACTGC CAATTTAAGA 
GAATATGCTA GATATGTACT GAGGACTATC TGTCAACAGG AATGGGTAGG AGAACATTGC 
CTAAAAGAAC CTGAAAGGTT GTGCACAGAC AAAGAGCTTA TATTGGACCC TGTGCTTTCA 
AATATGCAAG CACAGAAATT ATTGCAGCTT ATTTGTTATC CTCATGGTAT TAAAGAATGT 
ACGGAGGGCG ACAATCTGCA GAGACAGCAC ATTAAGCGCA TTCTTCAGAA TCTTGAGCAG 
TGGACACTGA GGCAGTCCTG GCTAGAACTT CAGTTAATGA TCAAACAGTG CTTGAAGGAC 
CCTGGCTCTG GTTCAGTGGC TGAGATGAAC AACTTACTGG ACAATATTGC AAAGGCAACA 
ATAGAGGTAT TCCAGCAGTC TGCTGACTTG AACAATAATT CTTCTAATTC TGGAATGGGC 
CTCTTCAACC CAAATGGGAT TGCAACTACT GATGCTGGTA GCACAAGGCA GAATGGAATA 
AAGACATTTC TAAGTTCCTC TGAACGCAGG GGTGTATGGC TGGTGGCCCC CCTAATTGCC 
AGGCTGCCGA CCTCTGTGCA AGGAAGAGTA TTGAAAGCTG CAGGAGAGGA ACTGGAGAAG 
GGACAGCACT TGGGCTCTTC TTCAAAAAAG GAAAGAGACA GACAAAAACA GAAAAGTATG 
TCTCTTTTGA GTCAACAACC TTTCCTCTCA CTGGTCCTTA CCTGCCTTAA GGGACAAGAT 
GAACAACGGG AAGGCCTCCT AACATCTCTT CAGAATCAGG TTAATCAGAT TTTAAGTAAT 
TGGAGAGAAG AACGATACCA GGATGACATA AAAGCACGGC AGATGATGCA TGAAGCATTG 
CAACTTCGCC TAAATTTGGT AGGAGGAATG TTTGACACGG TGCAGAGGAG CACCCAGTGG 
ACAACAGACT GGGCCCTCCT CCTGCTTCAG ATCATAACTT CTGGAACTGT TGACATGCAC 
ACTAACAATG AATTATTCAC AACAGTTCTT GACATGCTGG GTGTGTTAAT CAATGGAACA 
TTAGCCTCAG ACCTATCAAA TGCATCCCCA GGGGGATCTG AAGAGAACAA ACGTGCATAC 
ATGAATTTAG TAAAGAAACT GAAGAAAGAG CTAGGAGACA AGAGATCAGA AAGTATTGAC 
AAAGTTCGAC AGTTGCTACC TTTGCCAAAA CAGACATGTG ATGTCATTAC TTGTGAACCT 
ATGGGTTCCT TGATTGACAC AAAGGGAAAC AAAATTGCTG GATTCGACTC CATAGATAAA 
AAACAGGGTC TTCAGGTGTC TACAAAGCAG AAGGTGTCCC CATGGGATTT GTTTGAGGGT 
CAGAAGAACC CAGCTCCTCT GTCTTGGGCC TGGTTCGGGA CTGTCCGAGT GGACCGAAAG 
GTGATCAAGT ACGAGGAGCA GCATCACCTG CTGTTGTATC ACACACACCC CATGCCCAAG 
CCCCGAAGTT ACTACCTCGA ACCGCTGCCC CTGCCTCCCG AGGAGGAAGA GGAGGAGCCC 
ACATCTCCCA TCTCTCAGGA ACCAGAGCGG AAATCAGCTG AGCTGTCAGA TCAGGGAAAA 
ACCGCAACAG ATGAAGAGAA GAAAACAAAA GGAAGGAAGC GCAAGACGAA ATCAAGCTCA 
AGAGTTGACG AGTATCCACA GAGCAACATA TACCGAGTGC CTCCTAACTA CTCACCCATT 
TCCTCCCAGA TGATGCACCA TCCGCAGTCC ACCTTATGGG GCTATAACCT CATGGGTCAG 
CCCCAGCAGC CGGGTTTTTT CCTTCAGAAC CAATCTCTTA CTCCAGGTGG TTCTAGATTG 
GACCCCACGG GCTCCTTCGT CCCAACGAAT ACCAAACAAG CTCTGTCCAA CATGCTGCAG 
CGGCGCTCGG GGGCCATGAT GCAGCCGCCG CCCCTCCACG CCATCACATC GCAGCAGCAG 
CTGATACAGA TGAAGCTTCT GCAGCAGCAG CAGCAGCAGC GGCTTCTCCG GCAAGCCCAG 
GCTCGACCTT TCCAGCAGGG CCCGCCAGGG GACCAGGCTG CTCTCTTTGC TGCACAAGCA 
CGGCCCTCCC CTCAGCTCCC CCAGTATCCA GGGCTGCAGC AAGCACAGAC CATGCCCCAG 
GGCTATACGA TGTATGGAAC GCAGATGCCT TTGCAGCAGA CCCCTCAGCA GCAGGCTGGT 
GGCGTAGTCC TGTCTCCTAG CTATAACTCC AGGACCTATC CGGCCACACA TTCCAACCCG 
GCGCTAATGG AAAGACTCAG ACAGATGCAG CAGCAGCCCA GTGGCTATGT TCAGCAGCAG 
GCGTCACCGT ACCTGCAGCC CTTGACCAGC TCTCAGAGAC TAAATCATCA GTCGCTTCAG 
CAGAGCCCTC TGGTAGGTGG AGGAATCGAT GCAGTGCTCA CTCCCACGCA TCCAAGCCTC 
CCCTCGGTGC CCCTGTCTCA GGACCCAATG AGAACCAGAC AGCCGCAGGT TCGACAGCAG 
CAGAGACTCC TCCAGATGCA GCAGCCCCCC CAGCCCCAGC AGCCCCCGCA GCCCCAGAGT 
CAGACCCTTG GTCTCCAAGC GATGCAGCCC CAGCAGCCTT TGTTTCCCAG GCAAGGCTTG 
CAGCAGACCC AGCAGCAGCA ACAGACGGCC GCCCTGGTGC GGCAGCTCCA GAAGCAGCTT 
TCTAGTAACC AGCCACAGCA AGGAGTGACT CCCTATGGGC ATCCATCACA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MED12L cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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