ROBO1 gene cDNA ORF clone, Felis catus(Domestic cat)

mRNA	Protein	Name
XM_019839545.1	XP_019695104.1	roundabout homolog 1 isoform X3
XM_019839547.1	XP_019695106.1	roundabout homolog 1 isoform X5
XM_019839548.1	XP_019695107.1	roundabout homolog 1 isoform X6
XM_019839542.1	XP_019695101.1	roundabout homolog 1 isoform X1
XM_019839549.1	XP_019695108.1	roundabout homolog 1 isoform X7
XM_019839543.1	XP_019695102.1	roundabout homolog 1 isoform X2
XM_019839546.1	XP_019695105.1	roundabout homolog 1 isoform X4
XM_019839543.2	XP_019695102.2	LOW QUALITY PROTEIN: roundabout homolog 1
XM_019839550.1	XP_019695109.1	roundabout homolog 1 isoform X8
XM_019839551.1	XP_019695110.1	roundabout homolog 1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb48387	XM_019839542.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48388	XM_019839543.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48389	XM_019839545.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48390	XM_019839546.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48391	XM_019839547.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48392	XM_019839548.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48393	XM_019839549.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48394	XM_019839550.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb48395	XM_019839551.1 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb60452	XM_019839543.2 Latest version!	Felis catus roundabout guidance receptor 1 (ROBO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ROBO1 ( XM_019839542.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695101.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb48387
Clone ID Related Accession (Same CDS sequence)	XM_019839542.1
Accession Version	XM_019839542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGGCGAACT GTAACTTTTC AGTGTGAAGC AACTGGAAAT 
CCTCAACCAG CTATTTTTTG GAGACGAGAG GGGAGTCAGA ATTTGCTTTT CTCATATCAG 
CCACCACAGT CAACCAGCCG TTTTTCAGTC TCCCAGACTG GTGATCTCAC TATCACTAAC 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC TGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTAACTGAT GTGATTGCAG ACCGGCCTCC CCCGGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTGGCC GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGCACC CACGATTCTT TGGAGAAAGG ATGGAGTGCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACA GGAGTGCTGC AGATCCGATA TGCTAAGCTG 
GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAGGC AACATGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACTGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACGGTA 
ACCTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA CATTATAGAA 
GCCTTCAGCC ACGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACGGAA 
ACATTCGCCA TTAAAGGACT AAAACCTAAT GCAATTTACC TTTTCCTTGT GAGAGCAGCA 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC GCAAGATGTT 
CCCCCAACGA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAATGTTGTA 
CTGCACCTGC ACAACCCCAC CATCCTTTCT TCCTCTTCAA TCGAAGTACA CTGGACAGTG 
GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC AGGAGCCAAC 
CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTT 
ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAGTTC GCAAAGACCT TAGAAGAAGC ACCCAGTGCC 
CCACCCCAAA GTGTAACTGT GTCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGGCGC CTCCAGAAGA CACTCAAAAC GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTAGATA TCATATAAAC AAGACAGTAG ATGGTTCCAC TTTTTCTGTG 
GTCATCCCTT CTCTGGTTCC TGGGATCCGG TACAGCATAG AGGTGGCAGC CAGCACTGGG 
GCCGGGGCCG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCT 
GTGTCGCCTG AAGACCAGGT CAGCCTTGCT CAGCAGATCT CCGATGTGGT GAAGCAGCCA 
GCGTTCATTG CCGGAATCGG GGCAGCCTGC TGGATCATCC TCATGGTGTT TAGCATCTGG 
CTTTATCGGC ACCGCAAGAA GAGAAACGGG CTGACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGCGGTGA AGCTGTAAGC 
AGTGGAGGGA GGCCAGGACT TCTGAACATC GGTGAACCTG CCACACAGCC ATGGCTGGCC 
GACACATGGC CTAATACTGG CAACAGTCAC AATGACTGCT CCATCACTTG CTGCACTGCA 
GCCAATGGTA ACAGTGATAG CAACCTGACC ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGACG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CTCCTTACGC CACCACCCAG 
CTCATCCAGT CCAACCTCAG CAACAACATG AACAATGGCA GTGGGGACCC CAGCGAGAAG 
CACTGGAAGC CACCTGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGA GCAAATGACA CAATTCCTCC AACTATCCCA 
TACAACCAGT CATACGACCA GAATACAGGA GGATCTTACA ACAGTTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAAGG GCACAAGAAA GGGGCACGGA CACCCAAAGT TCCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC GCCTCACAGC 
AATAGTGAAG AGTACAGCAT TTCTGTAGAT GAAAGTTATG ACCAAGAAAT GCCGTGTCCT 
GTGCCACCAG CCAGGATGTA TTTGCAACAG GACGAACTGG AAGAGGAGGA AGATGAACGA 
GGCCCCACCC CCCCTGTACG GGGAGCAGCT TCTTCTCCAG CTGCTGTGTC TTACAGCCAT 
CAGTCCACTG CGACACTGAC CCCGTCTCCA CAAGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGCCCAGAGG AGATTGGCCA CATGCAGCAC CAACCCGATA GGAGACGGCA GCCTGTGAGT 
CCCCCTCCGC CACCACGACC GATTTCCCCA CCACATACCT ATGGCTACAT TTCAGGACCT 
CTTGTCTCAG ATATGGATAC GGATGCTCCA GAAGAGGAAG AAGATGAAGC AGACATGGAA 
GTGGCCAAGA TGCAGACCAG ACGGCTTTTG TTACGTGGGC TTGAGCAGAC CCCTGCCTCC 
AGTGTAGGGG ACCTCGAGAG CTCTGTCACT GGGTCCATGA TCAATGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGTGGACGC TCCAGTGTTA GTTCTTCCGA TGGCTCCTTC 
TTCACCGATG CCGATTTTGC CCAGGCAGTG GCGGCAGCAG CAGAGTATGC TGGCCTCAAA 
GTGGCACGTC GCCAAATGCA GGATGCCGCT GGCCGCCGAC ATTTTCATGC ATCCCAGTGC 
CCAAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACATGA GTGCTGCGGT AATACAGAAG 
GCCAGACCAG CCAAGAAGCA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCTCC AGTGCCACCA CCTGCTATAA AGTCACCCAC TGTTCAATCC 
AAGGCACAGC TGGAGGTACG ACCTGTAATG GCACCCAAAC TACCTTCTAT AGAAGCAAGA 
ACAGACAGGT CATCAGATAG AAAAGTAGGC AGCTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CAGATATGAG AACAAATCCA GGTGATACCA GAGAAGCACA GGAGCAGCAA 
AATGATGGGA AAGGACGAGG AAACAAGGCA GCAAAACGCG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTTCTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695101.1
CDS	146..5110
Translation	MKWKHVPFLVIISLLSLTEDHLFLAQLIPDPEDLERGNDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839542.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839542.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGGCGAACT GTAACTTTTC AGTGTGAAGC AACTGGAAAT 
CCTCAACCAG CTATTTTTTG GAGACGAGAG GGGAGTCAGA ATTTGCTTTT CTCATATCAG 
CCACCACAGT CAACCAGCCG TTTTTCAGTC TCCCAGACTG GTGATCTCAC TATCACTAAC 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC TGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTAACTGAT GTGATTGCAG ACCGGCCTCC CCCGGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTGGCC GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGCACC CACGATTCTT TGGAGAAAGG ATGGAGTGCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACA GGAGTGCTGC AGATCCGATA TGCTAAGCTG 
GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAGGC AACATGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACTGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACGGTA 
ACCTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA CATTATAGAA 
GCCTTCAGCC ACGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACGGAA 
ACATTCGCCA TTAAAGGACT AAAACCTAAT GCAATTTACC TTTTCCTTGT GAGAGCAGCA 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC GCAAGATGTT 
CCCCCAACGA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAATGTTGTA 
CTGCACCTGC ACAACCCCAC CATCCTTTCT TCCTCTTCAA TCGAAGTACA CTGGACAGTG 
GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC AGGAGCCAAC 
CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTT 
ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAGTTC GCAAAGACCT TAGAAGAAGC ACCCAGTGCC 
CCACCCCAAA GTGTAACTGT GTCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGGCGC CTCCAGAAGA CACTCAAAAC GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTAGATA TCATATAAAC AAGACAGTAG ATGGTTCCAC TTTTTCTGTG 
GTCATCCCTT CTCTGGTTCC TGGGATCCGG TACAGCATAG AGGTGGCAGC CAGCACTGGG 
GCCGGGGCCG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCT 
GTGTCGCCTG AAGACCAGGT CAGCCTTGCT CAGCAGATCT CCGATGTGGT GAAGCAGCCA 
GCGTTCATTG CCGGAATCGG GGCAGCCTGC TGGATCATCC TCATGGTGTT TAGCATCTGG 
CTTTATCGGC ACCGCAAGAA GAGAAACGGG CTGACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGCGGTGA AGCTGTAAGC 
AGTGGAGGGA GGCCAGGACT TCTGAACATC GGTGAACCTG CCACACAGCC ATGGCTGGCC 
GACACATGGC CTAATACTGG CAACAGTCAC AATGACTGCT CCATCACTTG CTGCACTGCA 
GCCAATGGTA ACAGTGATAG CAACCTGACC ACCTACAGTC GCCCAGCTGA TTGTATAGCA 
AATTATAACA ACCAACTGGA TAACAAACAA ACAAATCTGA TGCTCCCTGA GTCAACTGTT 
TATGGTGATG TGGACCTTAG TAACAAAATC AATGAGATGA AAACCTTCAA TAGCCCAAAT 
CTGAAGGACG GGCGTTTTGT CAATCCATCA GGGCAGCCCA CTCCTTACGC CACCACCCAG 
CTCATCCAGT CCAACCTCAG CAACAACATG AACAATGGCA GTGGGGACCC CAGCGAGAAG 
CACTGGAAGC CACCTGGACA GCAGAAACAG GAAGTGGCAC CAATTCAGTA TAACATCATG 
GAGCAAAACA AACTGAACAA AGATTATCGA GCAAATGACA CAATTCCTCC AACTATCCCA 
TACAACCAGT CATACGACCA GAATACAGGA GGATCTTACA ACAGTTCAGA CCGGGGCAGT 
AGTACATCTG GGAGTCAAGG GCACAAGAAA GGGGCACGGA CACCCAAAGT TCCAAAACAG 
GGTGGCATGA ACTGGGCAGA CCTGCTTCCT CCTCCCCCAG CACATCCTCC GCCTCACAGC 
AATAGTGAAG AGTACAGCAT TTCTGTAGAT GAAAGTTATG ACCAAGAAAT GCCGTGTCCT 
GTGCCACCAG CCAGGATGTA TTTGCAACAG GACGAACTGG AAGAGGAGGA AGATGAACGA 
GGCCCCACCC CCCCTGTACG GGGAGCAGCT TCTTCTCCAG CTGCTGTGTC TTACAGCCAT 
CAGTCCACTG CGACACTGAC CCCGTCTCCA CAAGAAGAAC TCCAGCCCAT GTTACAGGAT 
TGCCCAGAGG AGATTGGCCA CATGCAGCAC CAACCCGATA GGAGACGGCA GCCTGTGAGT 
CCCCCTCCGC CACCACGACC GATTTCCCCA CCACATACCT ATGGCTACAT TTCAGGACCT 
CTTGTCTCAG ATATGGATAC GGATGCTCCA GAAGAGGAAG AAGATGAAGC AGACATGGAA 
GTGGCCAAGA TGCAGACCAG ACGGCTTTTG TTACGTGGGC TTGAGCAGAC CCCTGCCTCC 
AGTGTAGGGG ACCTCGAGAG CTCTGTCACT GGGTCCATGA TCAATGGCTG GGGCTCAGCC 
TCAGAGGAGG ACAACATTTC CAGTGGACGC TCCAGTGTTA GTTCTTCCGA TGGCTCCTTC 
TTCACCGATG CCGATTTTGC CCAGGCAGTG GCGGCAGCAG CAGAGTATGC TGGCCTCAAA 
GTGGCACGTC GCCAAATGCA GGATGCCGCT GGCCGCCGAC ATTTTCATGC ATCCCAGTGC 
CCAAGACCCA CAAGTCCTGT TTCTACAGAC AGCAACATGA GTGCTGCGGT AATACAGAAG 
GCCAGACCAG CCAAGAAGCA AAAACACCAG CCAGGACATC TGCGCAGAGA AGCCTACACA 
GATGATCTTC CACCTCCTCC AGTGCCACCA CCTGCTATAA AGTCACCCAC TGTTCAATCC 
AAGGCACAGC TGGAGGTACG ACCTGTAATG GCACCCAAAC TACCTTCTAT AGAAGCAAGA 
ACAGACAGGT CATCAGATAG AAAAGTAGGC AGCTACAAGG GGAGAGAAGT ATTGGATGGA 
AGACAAGTTG CAGATATGAG AACAAATCCA GGTGATACCA GAGAAGCACA GGAGCAGCAA 
AATGATGGGA AAGGACGAGG AAACAAGGCA GCAAAACGCG ACCTTCCACC AGCAAAGACT 
CATCTCATCC AAGAGGATAT TCTACCTTAC TGTAGACCTA CTTTTCCGAC ATCAAATAAT 
CCCAGAGATC CTAGTTCTTC AAGCTCAATG TCATCAAGAG GATCAGGAAG CAGACAAAGA 
GAACAAGCAA ATGTAGGTCG AAGAAATATT GCAGAAATGC AGGTTCTTGG AGGATATGAA 
AGAGGAGAAG ATAATAATGA AGAATTAGAG GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839543.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695102.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OFb48388
Clone ID Related Accession (Same CDS sequence)	XM_019839543.1
Accession Version	XM_019839543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGGCGAAC TGTAACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA 
GCTATTTTTT GGAGACGAGA GGGGAGTCAG AATTTGCTTT TCTCATATCA GCCACCACAG 
TCAACCAGCC GTTTTTCAGT CTCCCAGACT GGTGATCTCA CTATCACTAA CGTCCAGCGA 
TCTGATGTTG GTTATTATAT CTGCCAGACT TTAAATGTTG CTGGAAGTAT CATCACAAAA 
GCATATTTGG AAGTAACTGA TGTGATTGCA GACCGGCCTC CCCCGGTCAT TCGACAAGGT 
CCTGTGAATC AGACTGTGGC CGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC 
AGTCCAGCAC CCACGATTCT TTGGAGAAAG GATGGAGTGC TTGTTTCAAC CCAAGATTCT 
CGAATCAAAC AGCTGGAGAC AGGAGTGCTG CAGATCCGAT ATGCTAAGCT GGGTGACACA 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAGG CAACATGGAG TGCTTACATT 
GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACTGACCC AAATTTAATC 
CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCA GAAATACGGT AACCTTGTCA 
TGGCAACCAA ATTTGAATTC AGGTGCAACT CCAACATCTT ACATTATAGA AGCCTTCAGC 
CACGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACGGA AACATTCGCC 
ATTAAAGGAC TAAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGAGCAGC AAATGCATAT 
GGAATTAGTG ATCCAAGCCA GATATCAGAT CCAGTGAAAA CGCAAGATGT TCCCCCAACG 
AGTCAGGGAG TAGATCACAA GCAGGTCCAG AGAGAATTGG GAAATGTTGT ACTGCACCTG 
CACAACCCCA CCATCCTTTC TTCCTCTTCA ATCGAAGTAC ACTGGACAGT GGACCAACAA 
TCTCAGTATA TTCAAGGATA TAAAATTCTT TATCGGCCAT CAGGAGCCAA CCATGGTGAA 
TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT TATCCCTGAT 
CTCAAAAAGG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATTAAGTT CGCAAAGACC TTAGAAGAAG CACCCAGTGC CCCACCCCAA 
AGTGTAACTG TGTCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGGCG 
CCTCCAGAAG ACACTCAAAA CGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTAGAT ATCATATAAA CAAGACAGTA GATGGTTCCA CTTTTTCTGT GGTCATCCCT 
TCTCTGGTTC CTGGGATCCG GTACAGCATA GAGGTGGCAG CCAGCACTGG GGCCGGGGCC 
GGGGTGAAGA GCGAGCCACA GTTCATCCAG TTAGATTCTC ATGGAAACCC TGTGTCGCCT 
GAAGACCAGG TCAGCCTTGC TCAGCAGATC TCCGATGTGG TGAAGCAGCC AGCGTTCATT 
GCCGGAATCG GGGCAGCCTG CTGGATCATC CTCATGGTGT TTAGCATCTG GCTTTATCGG 
CACCGCAAGA AGAGAAACGG GCTGACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGCGGTG AAGCTGTAAG CAGTGGAGGG 
AGGCCAGGAC TTCTGAACAT CGGTGAACCT GCCACACAGC CATGGCTGGC CGACACATGG 
CCTAATACTG GCAACAGTCA CAATGACTGC TCCATCACTT GCTGCACTGC AGCCAATGGT 
AACAGTGATA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAC 
GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCTTACG CCACCACCCA GCTCATCCAG 
TCCAACCTCA GCAACAACAT GAACAATGGC AGTGGGGACC CCAGCGAGAA GCACTGGAAG 
CCACCTGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG AGCAAATGAC ACAATTCCTC CAACTATCCC ATACAACCAG 
TCATACGACC AGAATACAGG AGGATCTTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TTCCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CGCCTCACAG CAATAGTGAA 
GAGTACAGCA TTTCTGTAGA TGAAAGTTAT GACCAAGAAA TGCCGTGTCC TGTGCCACCA 
GCCAGGATGT ATTTGCAACA GGACGAACTG GAAGAGGAGG AAGATGAACG AGGCCCCACC 
CCCCCTGTAC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTACAGCCA TCAGTCCACT 
GCGACACTGA CCCCGTCTCC ACAAGAAGAA CTCCAGCCCA TGTTACAGGA TTGCCCAGAG 
GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCCCCTCCG 
CCACCACGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA 
GATATGGATA CGGATGCTCC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG 
ATGCAGACCA GACGGCTTTT GTTACGTGGG CTTGAGCAGA CCCCTGCCTC CAGTGTAGGG 
GACCTCGAGA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGTGGACG CTCCAGTGTT AGTTCTTCCG ATGGCTCCTT CTTCACCGAT 
GCCGATTTTG CCCAGGCAGT GGCGGCAGCA GCAGAGTATG CTGGCCTCAA AGTGGCACGT 
CGCCAAATGC AGGATGCCGC TGGCCGCCGA CATTTTCATG CATCCCAGTG CCCAAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACATG AGTGCTGCGG TAATACAGAA GGCCAGACCA 
GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCTC CAGTGCCACC ACCTGCTATA AAGTCACCCA CTGTTCAATC CAAGGCACAG 
CTGGAGGTAC GACCTGTAAT GGCACCCAAA CTACCTTCTA TAGAAGCAAG AACAGACAGG 
TCATCAGATA GAAAAGTAGG CAGCTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCAGATATGA GAACAAATCC AGGTGATACC AGAGAAGCAC AGGAGCAGCA AAATGATGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGC GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTTCTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695102.1
CDS	146..5101
Translation	MKWKHVPFLVIISLLSLTEDHLFLAQLIPDPEDLERGNDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839543.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839543.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGGCGAAC TGTAACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA 
GCTATTTTTT GGAGACGAGA GGGGAGTCAG AATTTGCTTT TCTCATATCA GCCACCACAG 
TCAACCAGCC GTTTTTCAGT CTCCCAGACT GGTGATCTCA CTATCACTAA CGTCCAGCGA 
TCTGATGTTG GTTATTATAT CTGCCAGACT TTAAATGTTG CTGGAAGTAT CATCACAAAA 
GCATATTTGG AAGTAACTGA TGTGATTGCA GACCGGCCTC CCCCGGTCAT TCGACAAGGT 
CCTGTGAATC AGACTGTGGC CGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC 
AGTCCAGCAC CCACGATTCT TTGGAGAAAG GATGGAGTGC TTGTTTCAAC CCAAGATTCT 
CGAATCAAAC AGCTGGAGAC AGGAGTGCTG CAGATCCGAT ATGCTAAGCT GGGTGACACA 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAGG CAACATGGAG TGCTTACATT 
GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACTGACCC AAATTTAATC 
CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCA GAAATACGGT AACCTTGTCA 
TGGCAACCAA ATTTGAATTC AGGTGCAACT CCAACATCTT ACATTATAGA AGCCTTCAGC 
CACGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACGGA AACATTCGCC 
ATTAAAGGAC TAAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGAGCAGC AAATGCATAT 
GGAATTAGTG ATCCAAGCCA GATATCAGAT CCAGTGAAAA CGCAAGATGT TCCCCCAACG 
AGTCAGGGAG TAGATCACAA GCAGGTCCAG AGAGAATTGG GAAATGTTGT ACTGCACCTG 
CACAACCCCA CCATCCTTTC TTCCTCTTCA ATCGAAGTAC ACTGGACAGT GGACCAACAA 
TCTCAGTATA TTCAAGGATA TAAAATTCTT TATCGGCCAT CAGGAGCCAA CCATGGTGAA 
TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT TATCCCTGAT 
CTCAAAAAGG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATTAAGTT CGCAAAGACC TTAGAAGAAG CACCCAGTGC CCCACCCCAA 
AGTGTAACTG TGTCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGGCG 
CCTCCAGAAG ACACTCAAAA CGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTAGAT ATCATATAAA CAAGACAGTA GATGGTTCCA CTTTTTCTGT GGTCATCCCT 
TCTCTGGTTC CTGGGATCCG GTACAGCATA GAGGTGGCAG CCAGCACTGG GGCCGGGGCC 
GGGGTGAAGA GCGAGCCACA GTTCATCCAG TTAGATTCTC ATGGAAACCC TGTGTCGCCT 
GAAGACCAGG TCAGCCTTGC TCAGCAGATC TCCGATGTGG TGAAGCAGCC AGCGTTCATT 
GCCGGAATCG GGGCAGCCTG CTGGATCATC CTCATGGTGT TTAGCATCTG GCTTTATCGG 
CACCGCAAGA AGAGAAACGG GCTGACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGCGGTG AAGCTGTAAG CAGTGGAGGG 
AGGCCAGGAC TTCTGAACAT CGGTGAACCT GCCACACAGC CATGGCTGGC CGACACATGG 
CCTAATACTG GCAACAGTCA CAATGACTGC TCCATCACTT GCTGCACTGC AGCCAATGGT 
AACAGTGATA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA AACAAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAC 
GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCTTACG CCACCACCCA GCTCATCCAG 
TCCAACCTCA GCAACAACAT GAACAATGGC AGTGGGGACC CCAGCGAGAA GCACTGGAAG 
CCACCTGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG AGCAAATGAC ACAATTCCTC CAACTATCCC ATACAACCAG 
TCATACGACC AGAATACAGG AGGATCTTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TTCCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CGCCTCACAG CAATAGTGAA 
GAGTACAGCA TTTCTGTAGA TGAAAGTTAT GACCAAGAAA TGCCGTGTCC TGTGCCACCA 
GCCAGGATGT ATTTGCAACA GGACGAACTG GAAGAGGAGG AAGATGAACG AGGCCCCACC 
CCCCCTGTAC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTACAGCCA TCAGTCCACT 
GCGACACTGA CCCCGTCTCC ACAAGAAGAA CTCCAGCCCA TGTTACAGGA TTGCCCAGAG 
GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCCCCTCCG 
CCACCACGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA 
GATATGGATA CGGATGCTCC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG 
ATGCAGACCA GACGGCTTTT GTTACGTGGG CTTGAGCAGA CCCCTGCCTC CAGTGTAGGG 
GACCTCGAGA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGTGGACG CTCCAGTGTT AGTTCTTCCG ATGGCTCCTT CTTCACCGAT 
GCCGATTTTG CCCAGGCAGT GGCGGCAGCA GCAGAGTATG CTGGCCTCAA AGTGGCACGT 
CGCCAAATGC AGGATGCCGC TGGCCGCCGA CATTTTCATG CATCCCAGTG CCCAAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACATG AGTGCTGCGG TAATACAGAA GGCCAGACCA 
GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCTC CAGTGCCACC ACCTGCTATA AAGTCACCCA CTGTTCAATC CAAGGCACAG 
CTGGAGGTAC GACCTGTAAT GGCACCCAAA CTACCTTCTA TAGAAGCAAG AACAGACAGG 
TCATCAGATA GAAAAGTAGG CAGCTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCAGATATGA GAACAAATCC AGGTGATACC AGAGAAGCAC AGGAGCAGCA AAATGATGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGC GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTTCTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839545.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695104.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

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CloneID	OFb48389
Clone ID Related Accession (Same CDS sequence)	XM_019839545.1
Accession Version	XM_019839545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGGCGAACT GTAACTTTTC AGTGTGAAGC AACTGGAAAT 
CCTCAACCAG CTATTTTTTG GAGACGAGAG GGGAGTCAGA ATTTGCTTTT CTCATATCAG 
CCACCACAGT CAACCAGCCG TTTTTCAGTC TCCCAGACTG GTGATCTCAC TATCACTAAC 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC TGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTAACTGAT GTGATTGCAG ACCGGCCTCC CCCGGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTGGCC GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGCACC CACGATTCTT TGGAGAAAGG ATGGAGTGCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACA GGAGTGCTGC AGATCCGATA TGCTAAGCTG 
GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAGGC AACATGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACTGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACGGTA 
ACCTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA CATTATAGAA 
GCCTTCAGCC ACGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACGGAA 
ACATTCGCCA TTAAAGGACT AAAACCTAAT GCAATTTACC TTTTCCTTGT GAGAGCAGCA 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC GCAAGATGTT 
CCCCCAACGA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAATGTTGTA 
CTGCACCTGC ACAACCCCAC CATCCTTTCT TCCTCTTCAA TCGAAGTACA CTGGACAGTG 
GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC AGGAGCCAAC 
CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTT 
ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAGTTC GCAAAGACCT TAGAAGAAGC ACCCAGTGCC 
CCACCCCAAA GTGTAACTGT GTCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGGCGC CTCCAGAAGA CACTCAAAAC GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTAGATA TCATATAAAC AAGACAGTAG ATGGTTCCAC TTTTTCTGTG 
GTCATCCCTT CTCTGGTTCC TGGGATCCGG TACAGCATAG AGGTGGCAGC CAGCACTGGG 
GCCGGGGCCG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCT 
GTGTCGCCTG AAGACCAGGT CAGCCTTGCT CAGCAGATCT CCGATGTGGT GAAGCAGCCA 
GCGTTCATTG CCGGAATCGG GGCAGCCTGC TGGATCATCC TCATGGTGTT TAGCATCTGG 
CTTTATCGGC ACCGCAAGAA GAGAAACGGG CTGACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTATC AGAGAGGCGG TGAAGCTGTA AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC 
ATCGGTGAAC CTGCCACACA GCCATGGCTG GCCGACACAT GGCCTAATAC TGGCAACAGT 
CACAATGACT GCTCCATCAC TTGCTGCACT GCAGCCAATG GTAACAGTGA TAGCAACCTG 
ACCACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ACGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACTCCTTA CGCCACCACC CAGCTCATCC AGTCCAACCT CAGCAACAAC 
ATGAACAATG GCAGTGGGGA CCCCAGCGAG AAGCACTGGA AGCCACCTGG ACAGCAGAAA 
CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT 
CGAGCAAATG ACACAATTCC TCCAACTATC CCATACAACC AGTCATACGA CCAGAATACA 
GGAGGATCTT ACAACAGTTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA AGGGCACAAG 
AAAGGGGCAC GGACACCCAA AGTTCCAAAA CAGGGTGGCA TGAACTGGGC AGACCTGCTT 
CCTCCTCCCC CAGCACATCC TCCGCCTCAC AGCAATAGTG AAGAGTACAG CATTTCTGTA 
GATGAAAGTT ATGACCAAGA AATGCCGTGT CCTGTGCCAC CAGCCAGGAT GTATTTGCAA 
CAGGACGAAC TGGAAGAGGA GGAAGATGAA CGAGGCCCCA CCCCCCCTGT ACGGGGAGCA 
GCTTCTTCTC CAGCTGCTGT GTCTTACAGC CATCAGTCCA CTGCGACACT GACCCCGTCT 
CCACAAGAAG AACTCCAGCC CATGTTACAG GATTGCCCAG AGGAGATTGG CCACATGCAG 
CACCAACCCG ATAGGAGACG GCAGCCTGTG AGTCCCCCTC CGCCACCACG ACCGATTTCC 
CCACCACATA CCTATGGCTA CATTTCAGGA CCTCTTGTCT CAGATATGGA TACGGATGCT 
CCAGAAGAGG AAGAAGATGA AGCAGACATG GAAGTGGCCA AGATGCAGAC CAGACGGCTT 
TTGTTACGTG GGCTTGAGCA GACCCCTGCC TCCAGTGTAG GGGACCTCGA GAGCTCTGTC 
ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGTGGA 
CGCTCCAGTG TTAGTTCTTC CGATGGCTCC TTCTTCACCG ATGCCGATTT TGCCCAGGCA 
GTGGCGGCAG CAGCAGAGTA TGCTGGCCTC AAAGTGGCAC GTCGCCAAAT GCAGGATGCC 
GCTGGCCGCC GACATTTTCA TGCATCCCAG TGCCCAAGAC CCACAAGTCC TGTTTCTACA 
GACAGCAACA TGAGTGCTGC GGTAATACAG AAGGCCAGAC CAGCCAAGAA GCAAAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC TCCAGTGCCA 
CCACCTGCTA TAAAGTCACC CACTGTTCAA TCCAAGGCAC AGCTGGAGGT ACGACCTGTA 
ATGGCACCCA AACTACCTTC TATAGAAGCA AGAACAGACA GGTCATCAGA TAGAAAAGTA 
GGCAGCTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCAGATAT GAGAACAAAT 
CCAGGTGATA CCAGAGAAGC ACAGGAGCAG CAAAATGATG GGAAAGGACG AGGAAACAAG 
GCAGCAAAAC GCGACCTTCC ACCAGCAAAG ACTCATCTCA TCCAAGAGGA TATTCTACCT 
TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGCTCA 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAAA TGCAGGTTCT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695104.1
CDS	146..5083
Translation	MKWKHVPFLVIISLLSLTEDHLFLAQLIPDPEDLERGNDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839545.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839545.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGGCGAACT GTAACTTTTC AGTGTGAAGC AACTGGAAAT 
CCTCAACCAG CTATTTTTTG GAGACGAGAG GGGAGTCAGA ATTTGCTTTT CTCATATCAG 
CCACCACAGT CAACCAGCCG TTTTTCAGTC TCCCAGACTG GTGATCTCAC TATCACTAAC 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC TGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTAACTGAT GTGATTGCAG ACCGGCCTCC CCCGGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTGGCC GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGCACC CACGATTCTT TGGAGAAAGG ATGGAGTGCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACA GGAGTGCTGC AGATCCGATA TGCTAAGCTG 
GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAGGC AACATGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACTGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACGGTA 
ACCTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA CATTATAGAA 
GCCTTCAGCC ACGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACGGAA 
ACATTCGCCA TTAAAGGACT AAAACCTAAT GCAATTTACC TTTTCCTTGT GAGAGCAGCA 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC GCAAGATGTT 
CCCCCAACGA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAATGTTGTA 
CTGCACCTGC ACAACCCCAC CATCCTTTCT TCCTCTTCAA TCGAAGTACA CTGGACAGTG 
GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC AGGAGCCAAC 
CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTT 
ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAGTTC GCAAAGACCT TAGAAGAAGC ACCCAGTGCC 
CCACCCCAAA GTGTAACTGT GTCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGGCGC CTCCAGAAGA CACTCAAAAC GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTAGATA TCATATAAAC AAGACAGTAG ATGGTTCCAC TTTTTCTGTG 
GTCATCCCTT CTCTGGTTCC TGGGATCCGG TACAGCATAG AGGTGGCAGC CAGCACTGGG 
GCCGGGGCCG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCT 
GTGTCGCCTG AAGACCAGGT CAGCCTTGCT CAGCAGATCT CCGATGTGGT GAAGCAGCCA 
GCGTTCATTG CCGGAATCGG GGCAGCCTGC TGGATCATCC TCATGGTGTT TAGCATCTGG 
CTTTATCGGC ACCGCAAGAA GAGAAACGGG CTGACTAGTA CTTACGCGGG TATCAGAAAA 
GTAACTTATC AGAGAGGCGG TGAAGCTGTA AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC 
ATCGGTGAAC CTGCCACACA GCCATGGCTG GCCGACACAT GGCCTAATAC TGGCAACAGT 
CACAATGACT GCTCCATCAC TTGCTGCACT GCAGCCAATG GTAACAGTGA TAGCAACCTG 
ACCACCTACA GTCGCCCAGC TGATTGTATA GCAAATTATA ACAACCAACT GGATAACAAA 
CAAACAAATC TGATGCTCCC TGAGTCAACT GTTTATGGTG ATGTGGACCT TAGTAACAAA 
ATCAATGAGA TGAAAACCTT CAATAGCCCA AATCTGAAGG ACGGGCGTTT TGTCAATCCA 
TCAGGGCAGC CCACTCCTTA CGCCACCACC CAGCTCATCC AGTCCAACCT CAGCAACAAC 
ATGAACAATG GCAGTGGGGA CCCCAGCGAG AAGCACTGGA AGCCACCTGG ACAGCAGAAA 
CAGGAAGTGG CACCAATTCA GTATAACATC ATGGAGCAAA ACAAACTGAA CAAAGATTAT 
CGAGCAAATG ACACAATTCC TCCAACTATC CCATACAACC AGTCATACGA CCAGAATACA 
GGAGGATCTT ACAACAGTTC AGACCGGGGC AGTAGTACAT CTGGGAGTCA AGGGCACAAG 
AAAGGGGCAC GGACACCCAA AGTTCCAAAA CAGGGTGGCA TGAACTGGGC AGACCTGCTT 
CCTCCTCCCC CAGCACATCC TCCGCCTCAC AGCAATAGTG AAGAGTACAG CATTTCTGTA 
GATGAAAGTT ATGACCAAGA AATGCCGTGT CCTGTGCCAC CAGCCAGGAT GTATTTGCAA 
CAGGACGAAC TGGAAGAGGA GGAAGATGAA CGAGGCCCCA CCCCCCCTGT ACGGGGAGCA 
GCTTCTTCTC CAGCTGCTGT GTCTTACAGC CATCAGTCCA CTGCGACACT GACCCCGTCT 
CCACAAGAAG AACTCCAGCC CATGTTACAG GATTGCCCAG AGGAGATTGG CCACATGCAG 
CACCAACCCG ATAGGAGACG GCAGCCTGTG AGTCCCCCTC CGCCACCACG ACCGATTTCC 
CCACCACATA CCTATGGCTA CATTTCAGGA CCTCTTGTCT CAGATATGGA TACGGATGCT 
CCAGAAGAGG AAGAAGATGA AGCAGACATG GAAGTGGCCA AGATGCAGAC CAGACGGCTT 
TTGTTACGTG GGCTTGAGCA GACCCCTGCC TCCAGTGTAG GGGACCTCGA GAGCTCTGTC 
ACTGGGTCCA TGATCAATGG CTGGGGCTCA GCCTCAGAGG AGGACAACAT TTCCAGTGGA 
CGCTCCAGTG TTAGTTCTTC CGATGGCTCC TTCTTCACCG ATGCCGATTT TGCCCAGGCA 
GTGGCGGCAG CAGCAGAGTA TGCTGGCCTC AAAGTGGCAC GTCGCCAAAT GCAGGATGCC 
GCTGGCCGCC GACATTTTCA TGCATCCCAG TGCCCAAGAC CCACAAGTCC TGTTTCTACA 
GACAGCAACA TGAGTGCTGC GGTAATACAG AAGGCCAGAC CAGCCAAGAA GCAAAAACAC 
CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC ACAGATGATC TTCCACCTCC TCCAGTGCCA 
CCACCTGCTA TAAAGTCACC CACTGTTCAA TCCAAGGCAC AGCTGGAGGT ACGACCTGTA 
ATGGCACCCA AACTACCTTC TATAGAAGCA AGAACAGACA GGTCATCAGA TAGAAAAGTA 
GGCAGCTACA AGGGGAGAGA AGTATTGGAT GGAAGACAAG TTGCAGATAT GAGAACAAAT 
CCAGGTGATA CCAGAGAAGC ACAGGAGCAG CAAAATGATG GGAAAGGACG AGGAAACAAG 
GCAGCAAAAC GCGACCTTCC ACCAGCAAAG ACTCATCTCA TCCAAGAGGA TATTCTACCT 
TACTGTAGAC CTACTTTTCC GACATCAAAT AATCCCAGAG ATCCTAGTTC TTCAAGCTCA 
ATGTCATCAA GAGGATCAGG AAGCAGACAA AGAGAACAAG CAAATGTAGG TCGAAGAAAT 
ATTGCAGAAA TGCAGGTTCT TGGAGGATAT GAAAGAGGAG AAGATAATAA TGAAGAATTA 
GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839546.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695105.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

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CloneID	OFb48390
Clone ID Related Accession (Same CDS sequence)	XM_019839546.1
Accession Version	XM_019839546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGTT TGGGAACAAC ATTCGTGAGT GAAACATCAT GTCATGGTAA AAGTGCTGAC 
CCTGAAGACC TAGAGAGGGG GAACGACAAC GGGACGCCCA TCCCCACCTC TGATAATGAT 
GACAATTCGC TGGGCTACAC AGGCTCCCGT CTTCGTCAGG AAGATTTTCC ACCTCGCATT 
GTTGAACACC CTTCGGACCT AATTGTGTCA AAAGGAGAAC CTGCAACTTT GAACTGTAAA 
GCTGAAGGCC GCCCCACACC CACTATCGAA TGGTACAAAG GGGGGGAGAG AGTGGAGACG 
GACAAAGATG ACCCTCGATC ACACCGAATG TTGCTGCCAA GTGGATCTTT ATTTTTCTTA 
CGTATAGTAC ATGGACGAAA AAGCAGGCCT GATGAAGGGG TCTACGTCTG TGTAGCAAGG 
AATTACCTCG GAGAGGCCGT GAGCCATAAT GCATCGCTGG AGGTGGCTAT ACTCCGGGAT 
GACTTCAGAC AAAATCCTTC CGATGTCATG GTGGCAGTGG GGGAGCCTGC AGTGATGGAA 
TGCCAGCCTC CACGAGGCCA TCCTGAACCC ACCATTTCAT GGAAGAAAGA TGGCTCTCCG 
CTGGATGACA AAGATGAACG AATAACTATT CGAGGAGGAA AGCTCATGAT CACTTACACC 
CGCAAAAGTG ATGCTGGCAA ATACGTTTGT GTTGGTACCA ACATGGTTGG GGAACGGGAG 
AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG AGACCATCGT TTGTAAAGAG ACCTAGTAAC 
TTGGCGGTAA CTGTGGACGA CAGTGCGGAA TTTAAATGTG AGGCCCGAGG TGACCCTGTG 
CCTACGGTAC GATGGAGGAA AGATGATGGA GAACTGCCCA AATCCAGATA TGAAATCAGA 
GATGATCATA CCTTGAAAAT TCGGAAGGTG ATGGCTGGTG ACATGGGGTC ATATACTTGT 
GTTGCGGAAA ATATGGTTGG AAAAGCGGAA GCATCAGCTA CTCTGACTGT CCAAGTTGGG 
TCTGAACCTC CACATTTTGT TGTGAAACCC CGTGACCAGG TTGTTGCTTT GGGGCGAACT 
GTAACTTTTC AGTGTGAAGC AACTGGAAAT CCTCAACCAG CTATTTTTTG GAGACGAGAG 
GGGAGTCAGA ATTTGCTTTT CTCATATCAG CCACCACAGT CAACCAGCCG TTTTTCAGTC 
TCCCAGACTG GTGATCTCAC TATCACTAAC GTCCAGCGAT CTGATGTTGG TTATTATATC 
TGCCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAAG CATATTTGGA AGTAACTGAT 
GTGATTGCAG ACCGGCCTCC CCCGGTCATT CGACAAGGTC CTGTGAATCA GACTGTGGCC 
GTGGATGGTA CTTTAGTGCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACGATTCTT 
TGGAGAAAGG ATGGAGTGCT TGTTTCAACC CAAGATTCTC GAATCAAACA GCTGGAGACA 
GGAGTGCTGC AGATCCGATA TGCTAAGCTG GGTGACACAG GTCGGTACAC CTGCATTGCA 
TCAACCCCCA GTGGTGAGGC AACATGGAGT GCTTACATTG AAGTCCAAGA ATTTGGAGTT 
CCAGTTCAGC CTCCAAGACC CACTGACCCA AATTTAATCC CTAGTGCCCC CTCAAAACCT 
GAAGTTACAG ATGTCAGCAG AAATACGGTA ACCTTGTCAT GGCAACCAAA TTTGAATTCA 
GGTGCAACTC CAACATCTTA CATTATAGAA GCCTTCAGCC ACGCATCTGG TAGCAGCTGG 
CAGACCGTAG CAGAGAATGT GAAAACGGAA ACATTCGCCA TTAAAGGACT AAAACCTAAT 
GCAATTTACC TTTTCCTTGT GAGAGCAGCA AATGCATATG GAATTAGTGA TCCAAGCCAG 
ATATCAGATC CAGTGAAAAC GCAAGATGTT CCCCCAACGA GTCAGGGAGT AGATCACAAG 
CAGGTCCAGA GAGAATTGGG AAATGTTGTA CTGCACCTGC ACAACCCCAC CATCCTTTCT 
TCCTCTTCAA TCGAAGTACA CTGGACAGTG GACCAACAAT CTCAGTATAT TCAAGGATAT 
AAAATTCTTT ATCGGCCATC AGGAGCCAAC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA 
GTGAGGACCC CAACCAAAAA TAGTGTGGTT ATCCCTGATC TCAAAAAGGG AGTCAACTAT 
GAAATTAAGG CTCGTCCTTT TTTTAATGAA TTTCAAGGAG CAGATAGTGA AATTAAGTTC 
GCAAAGACCT TAGAAGAAGC ACCCAGTGCC CCACCCCAAA GTGTAACTGT GTCAAAGAAT 
GATGGAAATG GAACTGCAAT TCTTGTTAGT TGGCAGGCGC CTCCAGAAGA CACTCAAAAC 
GGAATGGTCC AAGAGTATAA GGTTTGGTGT CTGGGCAATG AAACTAGATA TCATATAAAC 
AAGACAGTAG ATGGTTCCAC TTTTTCTGTG GTCATCCCTT CTCTGGTTCC TGGGATCCGG 
TACAGCATAG AGGTGGCAGC CAGCACTGGG GCCGGGGCCG GGGTGAAGAG CGAGCCACAG 
TTCATCCAGT TAGATTCTCA TGGAAACCCT GTGTCGCCTG AAGACCAGGT CAGCCTTGCT 
CAGCAGATCT CCGATGTGGT GAAGCAGCCA GCGTTCATTG CCGGAATCGG GGCAGCCTGC 
TGGATCATCC TCATGGTGTT TAGCATCTGG CTTTATCGGC ACCGCAAGAA GAGAAACGGG 
CTGACTAGTA CTTACGCGGG TATCAGAAAA GTCCCGTCTT TTACCTTCAC ACCAACAGTA 
ACTTATCAGA GAGGCGGTGA AGCTGTAAGC AGTGGAGGGA GGCCAGGACT TCTGAACATC 
GGTGAACCTG CCACACAGCC ATGGCTGGCC GACACATGGC CTAATACTGG CAACAGTCAC 
AATGACTGCT CCATCACTTG CTGCACTGCA GCCAATGGTA ACAGTGATAG CAACCTGACC 
ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA 
GGGCAGCCCA CTCCTTACGC CACCACCCAG CTCATCCAGT CCAACCTCAG CAACAACATG 
AACAATGGCA GTGGGGACCC CAGCGAGAAG CACTGGAAGC CACCTGGACA GCAGAAACAG 
GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AACTGAACAA AGATTATCGA 
GCAAATGACA CAATTCCTCC AACTATCCCA TACAACCAGT CATACGACCA GAATACAGGA 
GGATCTTACA ACAGTTCAGA CCGGGGCAGT AGTACATCTG GGAGTCAAGG GCACAAGAAA 
GGGGCACGGA CACCCAAAGT TCCAAAACAG GGTGGCATGA ACTGGGCAGA CCTGCTTCCT 
CCTCCCCCAG CACATCCTCC GCCTCACAGC AATAGTGAAG AGTACAGCAT TTCTGTAGAT 
GAAAGTTATG ACCAAGAAAT GCCGTGTCCT GTGCCACCAG CCAGGATGTA TTTGCAACAG 
GACGAACTGG AAGAGGAGGA AGATGAACGA GGCCCCACCC CCCCTGTACG GGGAGCAGCT 
TCTTCTCCAG CTGCTGTGTC TTACAGCCAT CAGTCCACTG CGACACTGAC CCCGTCTCCA 
CAAGAAGAAC TCCAGCCCAT GTTACAGGAT TGCCCAGAGG AGATTGGCCA CATGCAGCAC 
CAACCCGATA GGAGACGGCA GCCTGTGAGT CCCCCTCCGC CACCACGACC GATTTCCCCA 
CCACATACCT ATGGCTACAT TTCAGGACCT CTTGTCTCAG ATATGGATAC GGATGCTCCA 
GAAGAGGAAG AAGATGAAGC AGACATGGAA GTGGCCAAGA TGCAGACCAG ACGGCTTTTG 
TTACGTGGGC TTGAGCAGAC CCCTGCCTCC AGTGTAGGGG ACCTCGAGAG CTCTGTCACT 
GGGTCCATGA TCAATGGCTG GGGCTCAGCC TCAGAGGAGG ACAACATTTC CAGTGGACGC 
TCCAGTGTTA GTTCTTCCGA TGGCTCCTTC TTCACCGATG CCGATTTTGC CCAGGCAGTG 
GCGGCAGCAG CAGAGTATGC TGGCCTCAAA GTGGCACGTC GCCAAATGCA GGATGCCGCT 
GGCCGCCGAC ATTTTCATGC ATCCCAGTGC CCAAGACCCA CAAGTCCTGT TTCTACAGAC 
AGCAACATGA GTGCTGCGGT AATACAGAAG GCCAGACCAG CCAAGAAGCA AAAACACCAG 
CCAGGACATC TGCGCAGAGA AGCCTACACA GATGATCTTC CACCTCCTCC AGTGCCACCA 
CCTGCTATAA AGTCACCCAC TGTTCAATCC AAGGCACAGC TGGAGGTACG ACCTGTAATG 
GCACCCAAAC TACCTTCTAT AGAAGCAAGA ACAGACAGGT CATCAGATAG AAAAGTAGGC 
AGCTACAAGG GGAGAGAAGT ATTGGATGGA AGACAAGTTG CAGATATGAG AACAAATCCA 
GGTGATACCA GAGAAGCACA GGAGCAGCAA AATGATGGGA AAGGACGAGG AAACAAGGCA 
GCAAAACGCG ACCTTCCACC AGCAAAGACT CATCTCATCC AAGAGGATAT TCTACCTTAC 
TGTAGACCTA CTTTTCCGAC ATCAAATAAT CCCAGAGATC CTAGTTCTTC AAGCTCAATG 
TCATCAAGAG GATCAGGAAG CAGACAAAGA GAACAAGCAA ATGTAGGTCG AAGAAATATT 
GCAGAAATGC AGGTTCTTGG AGGATATGAA AGAGGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695105.1
CDS	16..4950
Translation	MSRLGTTFVSETSCHGKSADPEDLERGNDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839546.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839546.1

ORF Insert Sequence:

ATGTCTCGTT TGGGAACAAC ATTCGTGAGT GAAACATCAT GTCATGGTAA AAGTGCTGAC 
CCTGAAGACC TAGAGAGGGG GAACGACAAC GGGACGCCCA TCCCCACCTC TGATAATGAT 
GACAATTCGC TGGGCTACAC AGGCTCCCGT CTTCGTCAGG AAGATTTTCC ACCTCGCATT 
GTTGAACACC CTTCGGACCT AATTGTGTCA AAAGGAGAAC CTGCAACTTT GAACTGTAAA 
GCTGAAGGCC GCCCCACACC CACTATCGAA TGGTACAAAG GGGGGGAGAG AGTGGAGACG 
GACAAAGATG ACCCTCGATC ACACCGAATG TTGCTGCCAA GTGGATCTTT ATTTTTCTTA 
CGTATAGTAC ATGGACGAAA AAGCAGGCCT GATGAAGGGG TCTACGTCTG TGTAGCAAGG 
AATTACCTCG GAGAGGCCGT GAGCCATAAT GCATCGCTGG AGGTGGCTAT ACTCCGGGAT 
GACTTCAGAC AAAATCCTTC CGATGTCATG GTGGCAGTGG GGGAGCCTGC AGTGATGGAA 
TGCCAGCCTC CACGAGGCCA TCCTGAACCC ACCATTTCAT GGAAGAAAGA TGGCTCTCCG 
CTGGATGACA AAGATGAACG AATAACTATT CGAGGAGGAA AGCTCATGAT CACTTACACC 
CGCAAAAGTG ATGCTGGCAA ATACGTTTGT GTTGGTACCA ACATGGTTGG GGAACGGGAG 
AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG AGACCATCGT TTGTAAAGAG ACCTAGTAAC 
TTGGCGGTAA CTGTGGACGA CAGTGCGGAA TTTAAATGTG AGGCCCGAGG TGACCCTGTG 
CCTACGGTAC GATGGAGGAA AGATGATGGA GAACTGCCCA AATCCAGATA TGAAATCAGA 
GATGATCATA CCTTGAAAAT TCGGAAGGTG ATGGCTGGTG ACATGGGGTC ATATACTTGT 
GTTGCGGAAA ATATGGTTGG AAAAGCGGAA GCATCAGCTA CTCTGACTGT CCAAGTTGGG 
TCTGAACCTC CACATTTTGT TGTGAAACCC CGTGACCAGG TTGTTGCTTT GGGGCGAACT 
GTAACTTTTC AGTGTGAAGC AACTGGAAAT CCTCAACCAG CTATTTTTTG GAGACGAGAG 
GGGAGTCAGA ATTTGCTTTT CTCATATCAG CCACCACAGT CAACCAGCCG TTTTTCAGTC 
TCCCAGACTG GTGATCTCAC TATCACTAAC GTCCAGCGAT CTGATGTTGG TTATTATATC 
TGCCAGACTT TAAATGTTGC TGGAAGTATC ATCACAAAAG CATATTTGGA AGTAACTGAT 
GTGATTGCAG ACCGGCCTCC CCCGGTCATT CGACAAGGTC CTGTGAATCA GACTGTGGCC 
GTGGATGGTA CTTTAGTGCT CAGCTGTGTG GCCACAGGCA GTCCAGCACC CACGATTCTT 
TGGAGAAAGG ATGGAGTGCT TGTTTCAACC CAAGATTCTC GAATCAAACA GCTGGAGACA 
GGAGTGCTGC AGATCCGATA TGCTAAGCTG GGTGACACAG GTCGGTACAC CTGCATTGCA 
TCAACCCCCA GTGGTGAGGC AACATGGAGT GCTTACATTG AAGTCCAAGA ATTTGGAGTT 
CCAGTTCAGC CTCCAAGACC CACTGACCCA AATTTAATCC CTAGTGCCCC CTCAAAACCT 
GAAGTTACAG ATGTCAGCAG AAATACGGTA ACCTTGTCAT GGCAACCAAA TTTGAATTCA 
GGTGCAACTC CAACATCTTA CATTATAGAA GCCTTCAGCC ACGCATCTGG TAGCAGCTGG 
CAGACCGTAG CAGAGAATGT GAAAACGGAA ACATTCGCCA TTAAAGGACT AAAACCTAAT 
GCAATTTACC TTTTCCTTGT GAGAGCAGCA AATGCATATG GAATTAGTGA TCCAAGCCAG 
ATATCAGATC CAGTGAAAAC GCAAGATGTT CCCCCAACGA GTCAGGGAGT AGATCACAAG 
CAGGTCCAGA GAGAATTGGG AAATGTTGTA CTGCACCTGC ACAACCCCAC CATCCTTTCT 
TCCTCTTCAA TCGAAGTACA CTGGACAGTG GACCAACAAT CTCAGTATAT TCAAGGATAT 
AAAATTCTTT ATCGGCCATC AGGAGCCAAC CATGGTGAAT CGGAGTGGTT AGTTTTTGAA 
GTGAGGACCC CAACCAAAAA TAGTGTGGTT ATCCCTGATC TCAAAAAGGG AGTCAACTAT 
GAAATTAAGG CTCGTCCTTT TTTTAATGAA TTTCAAGGAG CAGATAGTGA AATTAAGTTC 
GCAAAGACCT TAGAAGAAGC ACCCAGTGCC CCACCCCAAA GTGTAACTGT GTCAAAGAAT 
GATGGAAATG GAACTGCAAT TCTTGTTAGT TGGCAGGCGC CTCCAGAAGA CACTCAAAAC 
GGAATGGTCC AAGAGTATAA GGTTTGGTGT CTGGGCAATG AAACTAGATA TCATATAAAC 
AAGACAGTAG ATGGTTCCAC TTTTTCTGTG GTCATCCCTT CTCTGGTTCC TGGGATCCGG 
TACAGCATAG AGGTGGCAGC CAGCACTGGG GCCGGGGCCG GGGTGAAGAG CGAGCCACAG 
TTCATCCAGT TAGATTCTCA TGGAAACCCT GTGTCGCCTG AAGACCAGGT CAGCCTTGCT 
CAGCAGATCT CCGATGTGGT GAAGCAGCCA GCGTTCATTG CCGGAATCGG GGCAGCCTGC 
TGGATCATCC TCATGGTGTT TAGCATCTGG CTTTATCGGC ACCGCAAGAA GAGAAACGGG 
CTGACTAGTA CTTACGCGGG TATCAGAAAA GTCCCGTCTT TTACCTTCAC ACCAACAGTA 
ACTTATCAGA GAGGCGGTGA AGCTGTAAGC AGTGGAGGGA GGCCAGGACT TCTGAACATC 
GGTGAACCTG CCACACAGCC ATGGCTGGCC GACACATGGC CTAATACTGG CAACAGTCAC 
AATGACTGCT CCATCACTTG CTGCACTGCA GCCAATGGTA ACAGTGATAG CAACCTGACC 
ACCTACAGTC GCCCAGCTGA TTGTATAGCA AATTATAACA ACCAACTGGA TAACAAACAA 
ACAAATCTGA TGCTCCCTGA GTCAACTGTT TATGGTGATG TGGACCTTAG TAACAAAATC 
AATGAGATGA AAACCTTCAA TAGCCCAAAT CTGAAGGACG GGCGTTTTGT CAATCCATCA 
GGGCAGCCCA CTCCTTACGC CACCACCCAG CTCATCCAGT CCAACCTCAG CAACAACATG 
AACAATGGCA GTGGGGACCC CAGCGAGAAG CACTGGAAGC CACCTGGACA GCAGAAACAG 
GAAGTGGCAC CAATTCAGTA TAACATCATG GAGCAAAACA AACTGAACAA AGATTATCGA 
GCAAATGACA CAATTCCTCC AACTATCCCA TACAACCAGT CATACGACCA GAATACAGGA 
GGATCTTACA ACAGTTCAGA CCGGGGCAGT AGTACATCTG GGAGTCAAGG GCACAAGAAA 
GGGGCACGGA CACCCAAAGT TCCAAAACAG GGTGGCATGA ACTGGGCAGA CCTGCTTCCT 
CCTCCCCCAG CACATCCTCC GCCTCACAGC AATAGTGAAG AGTACAGCAT TTCTGTAGAT 
GAAAGTTATG ACCAAGAAAT GCCGTGTCCT GTGCCACCAG CCAGGATGTA TTTGCAACAG 
GACGAACTGG AAGAGGAGGA AGATGAACGA GGCCCCACCC CCCCTGTACG GGGAGCAGCT 
TCTTCTCCAG CTGCTGTGTC TTACAGCCAT CAGTCCACTG CGACACTGAC CCCGTCTCCA 
CAAGAAGAAC TCCAGCCCAT GTTACAGGAT TGCCCAGAGG AGATTGGCCA CATGCAGCAC 
CAACCCGATA GGAGACGGCA GCCTGTGAGT CCCCCTCCGC CACCACGACC GATTTCCCCA 
CCACATACCT ATGGCTACAT TTCAGGACCT CTTGTCTCAG ATATGGATAC GGATGCTCCA 
GAAGAGGAAG AAGATGAAGC AGACATGGAA GTGGCCAAGA TGCAGACCAG ACGGCTTTTG 
TTACGTGGGC TTGAGCAGAC CCCTGCCTCC AGTGTAGGGG ACCTCGAGAG CTCTGTCACT 
GGGTCCATGA TCAATGGCTG GGGCTCAGCC TCAGAGGAGG ACAACATTTC CAGTGGACGC 
TCCAGTGTTA GTTCTTCCGA TGGCTCCTTC TTCACCGATG CCGATTTTGC CCAGGCAGTG 
GCGGCAGCAG CAGAGTATGC TGGCCTCAAA GTGGCACGTC GCCAAATGCA GGATGCCGCT 
GGCCGCCGAC ATTTTCATGC ATCCCAGTGC CCAAGACCCA CAAGTCCTGT TTCTACAGAC 
AGCAACATGA GTGCTGCGGT AATACAGAAG GCCAGACCAG CCAAGAAGCA AAAACACCAG 
CCAGGACATC TGCGCAGAGA AGCCTACACA GATGATCTTC CACCTCCTCC AGTGCCACCA 
CCTGCTATAA AGTCACCCAC TGTTCAATCC AAGGCACAGC TGGAGGTACG ACCTGTAATG 
GCACCCAAAC TACCTTCTAT AGAAGCAAGA ACAGACAGGT CATCAGATAG AAAAGTAGGC 
AGCTACAAGG GGAGAGAAGT ATTGGATGGA AGACAAGTTG CAGATATGAG AACAAATCCA 
GGTGATACCA GAGAAGCACA GGAGCAGCAA AATGATGGGA AAGGACGAGG AAACAAGGCA 
GCAAAACGCG ACCTTCCACC AGCAAAGACT CATCTCATCC AAGAGGATAT TCTACCTTAC 
TGTAGACCTA CTTTTCCGAC ATCAAATAAT CCCAGAGATC CTAGTTCTTC AAGCTCAATG 
TCATCAAGAG GATCAGGAAG CAGACAAAGA GAACAAGCAA ATGTAGGTCG AAGAAATATT 
GCAGAAATGC AGGTTCTTGG AGGATATGAA AGAGGAGAAG ATAATAATGA AGAATTAGAG 
GAAACTGAAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839547.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695106.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

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CloneID	OFb48391
Clone ID Related Accession (Same CDS sequence)	XM_019839547.1
Accession Version	XM_019839547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGGCGA ACTGTAACTT TTCAGTGTGA AGCAACTGGA 
AATCCTCAAC CAGCTATTTT TTGGAGACGA GAGGGGAGTC AGAATTTGCT TTTCTCATAT 
CAGCCACCAC AGTCAACCAG CCGTTTTTCA GTCTCCCAGA CTGGTGATCT CACTATCACT 
AACGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTAACT GATGTGATTG CAGACCGGCC TCCCCCGGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTG GCCGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACGATT CTTTGGAGAA AGGATGGAGT GCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACAGGAGTGC TGCAGATCCG ATATGCTAAG 
CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA GGCAACATGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACTGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACG 
GTAACCTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTACATTATA 
GAAGCCTTCA GCCACGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACG 
GAAACATTCG CCATTAAAGG ACTAAAACCT AATGCAATTT ACCTTTTCCT TGTGAGAGCA 
GCAAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACGCAAGAT 
GTTCCCCCAA CGAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAATGTT 
GTACTGCACC TGCACAACCC CACCATCCTT TCTTCCTCTT CAATCGAAGT ACACTGGACA 
GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCAGGAGCC 
AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG 
GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAG TTCGCAAAGA CCTTAGAAGA AGCACCCAGT 
GCCCCACCCC AAAGTGTAAC TGTGTCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGG CGCCTCCAGA AGACACTCAA AACGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTAG ATATCATATA AACAAGACAG TAGATGGTTC CACTTTTTCT 
GTGGTCATCC CTTCTCTGGT TCCTGGGATC CGGTACAGCA TAGAGGTGGC AGCCAGCACT 
GGGGCCGGGG CCGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC 
CCTGTGTCGC CTGAAGACCA GGTCAGCCTT GCTCAGCAGA TCTCCGATGT GGTGAAGCAG 
CCAGCGTTCA TTGCCGGAAT CGGGGCAGCC TGCTGGATCA TCCTCATGGT GTTTAGCATC 
TGGCTTTATC GGCACCGCAA GAAGAGAAAC GGGCTGACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGCGG TGAAGCTGTA 
AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC ATCGGTGAAC CTGCCACACA GCCATGGCTG 
GCCGACACAT GGCCTAATAC TGGCAACAGT CACAATGACT GCTCCATCAC TTGCTGCACT 
GCAGCCAATG GTAACAGTGA TAGCAACCTG ACCACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCTTA CGCCACCACC 
CAGCTCATCC AGTCCAACCT CAGCAACAAC ATGAACAATG GCAGTGGGGA CCCCAGCGAG 
AAGCACTGGA AGCCACCTGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGAGCAAATG ACACAATTCC TCCAACTATC 
CCATACAACC AGTCATACGA CCAGAATACA GGAGGATCTT ACAACAGTTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA AGGGCACAAG AAAGGGGCAC GGACACCCAA AGTTCCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCGCCTCAC 
AGCAATAGTG AAGAGTACAG CATTTCTGTA GATGAAAGTT ATGACCAAGA AATGCCGTGT 
CCTGTGCCAC CAGCCAGGAT GTATTTGCAA CAGGACGAAC TGGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CCCCCCCTGT ACGGGGAGCA GCTTCTTCTC CAGCTGCTGT GTCTTACAGC 
CATCAGTCCA CTGCGACACT GACCCCGTCT CCACAAGAAG AACTCCAGCC CATGTTACAG 
GATTGCCCAG AGGAGATTGG CCACATGCAG CACCAACCCG ATAGGAGACG GCAGCCTGTG 
AGTCCCCCTC CGCCACCACG ACCGATTTCC CCACCACATA CCTATGGCTA CATTTCAGGA 
CCTCTTGTCT CAGATATGGA TACGGATGCT CCAGAAGAGG AAGAAGATGA AGCAGACATG 
GAAGTGGCCA AGATGCAGAC CAGACGGCTT TTGTTACGTG GGCTTGAGCA GACCCCTGCC 
TCCAGTGTAG GGGACCTCGA GAGCTCTGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGTGGA CGCTCCAGTG TTAGTTCTTC CGATGGCTCC 
TTCTTCACCG ATGCCGATTT TGCCCAGGCA GTGGCGGCAG CAGCAGAGTA TGCTGGCCTC 
AAAGTGGCAC GTCGCCAAAT GCAGGATGCC GCTGGCCGCC GACATTTTCA TGCATCCCAG 
TGCCCAAGAC CCACAAGTCC TGTTTCTACA GACAGCAACA TGAGTGCTGC GGTAATACAG 
AAGGCCAGAC CAGCCAAGAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC 
ACAGATGATC TTCCACCTCC TCCAGTGCCA CCACCTGCTA TAAAGTCACC CACTGTTCAA 
TCCAAGGCAC AGCTGGAGGT ACGACCTGTA ATGGCACCCA AACTACCTTC TATAGAAGCA 
AGAACAGACA GGTCATCAGA TAGAAAAGTA GGCAGCTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAAG TTGCAGATAT GAGAACAAAT CCAGGTGATA CCAGAGAAGC ACAGGAGCAG 
CAAAATGATG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GCGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TACTGTAGAC CTACTTTTCC GACATCAAAT 
AATCCCAGAG ATCCTAGTTC TTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTTCT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695106.1
CDS	1041..5888
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839547.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839547.1

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGGCGA ACTGTAACTT TTCAGTGTGA AGCAACTGGA 
AATCCTCAAC CAGCTATTTT TTGGAGACGA GAGGGGAGTC AGAATTTGCT TTTCTCATAT 
CAGCCACCAC AGTCAACCAG CCGTTTTTCA GTCTCCCAGA CTGGTGATCT CACTATCACT 
AACGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTAACT GATGTGATTG CAGACCGGCC TCCCCCGGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTG GCCGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACGATT CTTTGGAGAA AGGATGGAGT GCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACAGGAGTGC TGCAGATCCG ATATGCTAAG 
CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA GGCAACATGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACTGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACG 
GTAACCTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTACATTATA 
GAAGCCTTCA GCCACGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACG 
GAAACATTCG CCATTAAAGG ACTAAAACCT AATGCAATTT ACCTTTTCCT TGTGAGAGCA 
GCAAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACGCAAGAT 
GTTCCCCCAA CGAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAATGTT 
GTACTGCACC TGCACAACCC CACCATCCTT TCTTCCTCTT CAATCGAAGT ACACTGGACA 
GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCAGGAGCC 
AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG 
GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAG TTCGCAAAGA CCTTAGAAGA AGCACCCAGT 
GCCCCACCCC AAAGTGTAAC TGTGTCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGG CGCCTCCAGA AGACACTCAA AACGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTAG ATATCATATA AACAAGACAG TAGATGGTTC CACTTTTTCT 
GTGGTCATCC CTTCTCTGGT TCCTGGGATC CGGTACAGCA TAGAGGTGGC AGCCAGCACT 
GGGGCCGGGG CCGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC 
CCTGTGTCGC CTGAAGACCA GGTCAGCCTT GCTCAGCAGA TCTCCGATGT GGTGAAGCAG 
CCAGCGTTCA TTGCCGGAAT CGGGGCAGCC TGCTGGATCA TCCTCATGGT GTTTAGCATC 
TGGCTTTATC GGCACCGCAA GAAGAGAAAC GGGCTGACTA GTACTTACGC GGGTATCAGA 
AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACTTATC AGAGAGGCGG TGAAGCTGTA 
AGCAGTGGAG GGAGGCCAGG ACTTCTGAAC ATCGGTGAAC CTGCCACACA GCCATGGCTG 
GCCGACACAT GGCCTAATAC TGGCAACAGT CACAATGACT GCTCCATCAC TTGCTGCACT 
GCAGCCAATG GTAACAGTGA TAGCAACCTG ACCACCTACA GTCGCCCAGC TGATTGTATA 
GCAAATTATA ACAACCAACT GGATAACAAA CAAACAAATC TGATGCTCCC TGAGTCAACT 
GTTTATGGTG ATGTGGACCT TAGTAACAAA ATCAATGAGA TGAAAACCTT CAATAGCCCA 
AATCTGAAGG ACGGGCGTTT TGTCAATCCA TCAGGGCAGC CCACTCCTTA CGCCACCACC 
CAGCTCATCC AGTCCAACCT CAGCAACAAC ATGAACAATG GCAGTGGGGA CCCCAGCGAG 
AAGCACTGGA AGCCACCTGG ACAGCAGAAA CAGGAAGTGG CACCAATTCA GTATAACATC 
ATGGAGCAAA ACAAACTGAA CAAAGATTAT CGAGCAAATG ACACAATTCC TCCAACTATC 
CCATACAACC AGTCATACGA CCAGAATACA GGAGGATCTT ACAACAGTTC AGACCGGGGC 
AGTAGTACAT CTGGGAGTCA AGGGCACAAG AAAGGGGCAC GGACACCCAA AGTTCCAAAA 
CAGGGTGGCA TGAACTGGGC AGACCTGCTT CCTCCTCCCC CAGCACATCC TCCGCCTCAC 
AGCAATAGTG AAGAGTACAG CATTTCTGTA GATGAAAGTT ATGACCAAGA AATGCCGTGT 
CCTGTGCCAC CAGCCAGGAT GTATTTGCAA CAGGACGAAC TGGAAGAGGA GGAAGATGAA 
CGAGGCCCCA CCCCCCCTGT ACGGGGAGCA GCTTCTTCTC CAGCTGCTGT GTCTTACAGC 
CATCAGTCCA CTGCGACACT GACCCCGTCT CCACAAGAAG AACTCCAGCC CATGTTACAG 
GATTGCCCAG AGGAGATTGG CCACATGCAG CACCAACCCG ATAGGAGACG GCAGCCTGTG 
AGTCCCCCTC CGCCACCACG ACCGATTTCC CCACCACATA CCTATGGCTA CATTTCAGGA 
CCTCTTGTCT CAGATATGGA TACGGATGCT CCAGAAGAGG AAGAAGATGA AGCAGACATG 
GAAGTGGCCA AGATGCAGAC CAGACGGCTT TTGTTACGTG GGCTTGAGCA GACCCCTGCC 
TCCAGTGTAG GGGACCTCGA GAGCTCTGTC ACTGGGTCCA TGATCAATGG CTGGGGCTCA 
GCCTCAGAGG AGGACAACAT TTCCAGTGGA CGCTCCAGTG TTAGTTCTTC CGATGGCTCC 
TTCTTCACCG ATGCCGATTT TGCCCAGGCA GTGGCGGCAG CAGCAGAGTA TGCTGGCCTC 
AAAGTGGCAC GTCGCCAAAT GCAGGATGCC GCTGGCCGCC GACATTTTCA TGCATCCCAG 
TGCCCAAGAC CCACAAGTCC TGTTTCTACA GACAGCAACA TGAGTGCTGC GGTAATACAG 
AAGGCCAGAC CAGCCAAGAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGCCTAC 
ACAGATGATC TTCCACCTCC TCCAGTGCCA CCACCTGCTA TAAAGTCACC CACTGTTCAA 
TCCAAGGCAC AGCTGGAGGT ACGACCTGTA ATGGCACCCA AACTACCTTC TATAGAAGCA 
AGAACAGACA GGTCATCAGA TAGAAAAGTA GGCAGCTACA AGGGGAGAGA AGTATTGGAT 
GGAAGACAAG TTGCAGATAT GAGAACAAAT CCAGGTGATA CCAGAGAAGC ACAGGAGCAG 
CAAAATGATG GGAAAGGACG AGGAAACAAG GCAGCAAAAC GCGACCTTCC ACCAGCAAAG 
ACTCATCTCA TCCAAGAGGA TATTCTACCT TACTGTAGAC CTACTTTTCC GACATCAAAT 
AATCCCAGAG ATCCTAGTTC TTCAAGCTCA ATGTCATCAA GAGGATCAGG AAGCAGACAA 
AGAGAACAAG CAAATGTAGG TCGAAGAAAT ATTGCAGAAA TGCAGGTTCT TGGAGGATAT 
GAAAGAGGAG AAGATAATAA TGAAGAATTA GAGGAAACTG AAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839548.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695107.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

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CloneID	OFb48392
Clone ID Related Accession (Same CDS sequence)	XM_019839548.1
Accession Version	XM_019839548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGGCG AACTGTAACT TTTCAGTGTG AAGCAACTGG AAATCCTCAA 
CCAGCTATTT TTTGGAGACG AGAGGGGAGT CAGAATTTGC TTTTCTCATA TCAGCCACCA 
CAGTCAACCA GCCGTTTTTC AGTCTCCCAG ACTGGTGATC TCACTATCAC TAACGTCCAG 
CGATCTGATG TTGGTTATTA TATCTGCCAG ACTTTAAATG TTGCTGGAAG TATCATCACA 
AAAGCATATT TGGAAGTAAC TGATGTGATT GCAGACCGGC CTCCCCCGGT CATTCGACAA 
GGTCCTGTGA ATCAGACTGT GGCCGTGGAT GGTACTTTAG TGCTCAGCTG TGTGGCCACA 
GGCAGTCCAG CACCCACGAT TCTTTGGAGA AAGGATGGAG TGCTTGTTTC AACCCAAGAT 
TCTCGAATCA AACAGCTGGA GACAGGAGTG CTGCAGATCC GATATGCTAA GCTGGGTGAC 
ACAGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AGGCAACATG GAGTGCTTAC 
ATTGAAGTCC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCCACTGA CCCAAATTTA 
ATCCCTAGTG CCCCCTCAAA ACCTGAAGTT ACAGATGTCA GCAGAAATAC GGTAACCTTG 
TCATGGCAAC CAAATTTGAA TTCAGGTGCA ACTCCAACAT CTTACATTAT AGAAGCCTTC 
AGCCACGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC GGAAACATTC 
GCCATTAAAG GACTAAAACC TAATGCAATT TACCTTTTCC TTGTGAGAGC AGCAAATGCA 
TATGGAATTA GTGATCCAAG CCAGATATCA GATCCAGTGA AAACGCAAGA TGTTCCCCCA 
ACGAGTCAGG GAGTAGATCA CAAGCAGGTC CAGAGAGAAT TGGGAAATGT TGTACTGCAC 
CTGCACAACC CCACCATCCT TTCTTCCTCT TCAATCGAAG TACACTGGAC AGTGGACCAA 
CAATCTCAGT ATATTCAAGG ATATAAAATT CTTTATCGGC CATCAGGAGC CAACCATGGT 
GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAATAGTGT GGTTATCCCT 
GATCTCAAAA AGGGAGTCAA CTATGAAATT AAGGCTCGTC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATTAA GTTCGCAAAG ACCTTAGAAG AAGCACCCAG TGCCCCACCC 
CAAAGTGTAA CTGTGTCAAA GAATGATGGA AATGGAACTG CAATTCTTGT TAGTTGGCAG 
GCGCCTCCAG AAGACACTCA AAACGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTA GATATCATAT AAACAAGACA GTAGATGGTT CCACTTTTTC TGTGGTCATC 
CCTTCTCTGG TTCCTGGGAT CCGGTACAGC ATAGAGGTGG CAGCCAGCAC TGGGGCCGGG 
GCCGGGGTGA AGAGCGAGCC ACAGTTCATC CAGTTAGATT CTCATGGAAA CCCTGTGTCG 
CCTGAAGACC AGGTCAGCCT TGCTCAGCAG ATCTCCGATG TGGTGAAGCA GCCAGCGTTC 
ATTGCCGGAA TCGGGGCAGC CTGCTGGATC ATCCTCATGG TGTTTAGCAT CTGGCTTTAT 
CGGCACCGCA AGAAGAGAAA CGGGCTGACT AGTACTTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAT CAGAGAGGCG GTGAAGCTGT AAGCAGTGGA 
GGGAGGCCAG GACTTCTGAA CATCGGTGAA CCTGCCACAC AGCCATGGCT GGCCGACACA 
TGGCCTAATA CTGGCAACAG TCACAATGAC TGCTCCATCA CTTGCTGCAC TGCAGCCAAT 
GGTAACAGTG ATAGCAACCT GACCACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCTT ACGCCACCAC CCAGCTCATC 
CAGTCCAACC TCAGCAACAA CATGAACAAT GGCAGTGGGG ACCCCAGCGA GAAGCACTGG 
AAGCCACCTG GACAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAACTGA ACAAAGATTA TCGAGCAAAT GACACAATTC CTCCAACTAT CCCATACAAC 
CAGTCATACG ACCAGAATAC AGGAGGATCT TACAACAGTT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AAGGGCACAA GAAAGGGGCA CGGACACCCA AAGTTCCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCGCCTCA CAGCAATAGT 
GAAGAGTACA GCATTTCTGT AGATGAAAGT TATGACCAAG AAATGCCGTG TCCTGTGCCA 
CCAGCCAGGA TGTATTTGCA ACAGGACGAA CTGGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACCCCCCCTG TACGGGGAGC AGCTTCTTCT CCAGCTGCTG TGTCTTACAG CCATCAGTCC 
ACTGCGACAC TGACCCCGTC TCCACAAGAA GAACTCCAGC CCATGTTACA GGATTGCCCA 
GAGGAGATTG GCCACATGCA GCACCAACCC GATAGGAGAC GGCAGCCTGT GAGTCCCCCT 
CCGCCACCAC GACCGATTTC CCCACCACAT ACCTATGGCT ACATTTCAGG ACCTCTTGTC 
TCAGATATGG ATACGGATGC TCCAGAAGAG GAAGAAGATG AAGCAGACAT GGAAGTGGCC 
AAGATGCAGA CCAGACGGCT TTTGTTACGT GGGCTTGAGC AGACCCCTGC CTCCAGTGTA 
GGGGACCTCG AGAGCTCTGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGTGG ACGCTCCAGT GTTAGTTCTT CCGATGGCTC CTTCTTCACC 
GATGCCGATT TTGCCCAGGC AGTGGCGGCA GCAGCAGAGT ATGCTGGCCT CAAAGTGGCA 
CGTCGCCAAA TGCAGGATGC CGCTGGCCGC CGACATTTTC ATGCATCCCA GTGCCCAAGA 
CCCACAAGTC CTGTTTCTAC AGACAGCAAC ATGAGTGCTG CGGTAATACA GAAGGCCAGA 
CCAGCCAAGA AGCAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGCCTA CACAGATGAT 
CTTCCACCTC CTCCAGTGCC ACCACCTGCT ATAAAGTCAC CCACTGTTCA ATCCAAGGCA 
CAGCTGGAGG TACGACCTGT AATGGCACCC AAACTACCTT CTATAGAAGC AAGAACAGAC 
AGGTCATCAG ATAGAAAAGT AGGCAGCTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAA 
GTTGCAGATA TGAGAACAAA TCCAGGTGAT ACCAGAGAAG CACAGGAGCA GCAAAATGAT 
GGGAAAGGAC GAGGAAACAA GGCAGCAAAA CGCGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTACTGTAGA CCTACTTTTC CGACATCAAA TAATCCCAGA 
GATCCTAGTT CTTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTTC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695107.1
CDS	1041..5879
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839548.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839548.1

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGAA CCTCCACATT TTGTTGTGAA ACCCCGTGAC 
CAGGTTGTTG CTTTGGGGCG AACTGTAACT TTTCAGTGTG AAGCAACTGG AAATCCTCAA 
CCAGCTATTT TTTGGAGACG AGAGGGGAGT CAGAATTTGC TTTTCTCATA TCAGCCACCA 
CAGTCAACCA GCCGTTTTTC AGTCTCCCAG ACTGGTGATC TCACTATCAC TAACGTCCAG 
CGATCTGATG TTGGTTATTA TATCTGCCAG ACTTTAAATG TTGCTGGAAG TATCATCACA 
AAAGCATATT TGGAAGTAAC TGATGTGATT GCAGACCGGC CTCCCCCGGT CATTCGACAA 
GGTCCTGTGA ATCAGACTGT GGCCGTGGAT GGTACTTTAG TGCTCAGCTG TGTGGCCACA 
GGCAGTCCAG CACCCACGAT TCTTTGGAGA AAGGATGGAG TGCTTGTTTC AACCCAAGAT 
TCTCGAATCA AACAGCTGGA GACAGGAGTG CTGCAGATCC GATATGCTAA GCTGGGTGAC 
ACAGGTCGGT ACACCTGCAT TGCATCAACC CCCAGTGGTG AGGCAACATG GAGTGCTTAC 
ATTGAAGTCC AAGAATTTGG AGTTCCAGTT CAGCCTCCAA GACCCACTGA CCCAAATTTA 
ATCCCTAGTG CCCCCTCAAA ACCTGAAGTT ACAGATGTCA GCAGAAATAC GGTAACCTTG 
TCATGGCAAC CAAATTTGAA TTCAGGTGCA ACTCCAACAT CTTACATTAT AGAAGCCTTC 
AGCCACGCAT CTGGTAGCAG CTGGCAGACC GTAGCAGAGA ATGTGAAAAC GGAAACATTC 
GCCATTAAAG GACTAAAACC TAATGCAATT TACCTTTTCC TTGTGAGAGC AGCAAATGCA 
TATGGAATTA GTGATCCAAG CCAGATATCA GATCCAGTGA AAACGCAAGA TGTTCCCCCA 
ACGAGTCAGG GAGTAGATCA CAAGCAGGTC CAGAGAGAAT TGGGAAATGT TGTACTGCAC 
CTGCACAACC CCACCATCCT TTCTTCCTCT TCAATCGAAG TACACTGGAC AGTGGACCAA 
CAATCTCAGT ATATTCAAGG ATATAAAATT CTTTATCGGC CATCAGGAGC CAACCATGGT 
GAATCGGAGT GGTTAGTTTT TGAAGTGAGG ACCCCAACCA AAAATAGTGT GGTTATCCCT 
GATCTCAAAA AGGGAGTCAA CTATGAAATT AAGGCTCGTC CTTTTTTTAA TGAATTTCAA 
GGAGCAGATA GTGAAATTAA GTTCGCAAAG ACCTTAGAAG AAGCACCCAG TGCCCCACCC 
CAAAGTGTAA CTGTGTCAAA GAATGATGGA AATGGAACTG CAATTCTTGT TAGTTGGCAG 
GCGCCTCCAG AAGACACTCA AAACGGAATG GTCCAAGAGT ATAAGGTTTG GTGTCTGGGC 
AATGAAACTA GATATCATAT AAACAAGACA GTAGATGGTT CCACTTTTTC TGTGGTCATC 
CCTTCTCTGG TTCCTGGGAT CCGGTACAGC ATAGAGGTGG CAGCCAGCAC TGGGGCCGGG 
GCCGGGGTGA AGAGCGAGCC ACAGTTCATC CAGTTAGATT CTCATGGAAA CCCTGTGTCG 
CCTGAAGACC AGGTCAGCCT TGCTCAGCAG ATCTCCGATG TGGTGAAGCA GCCAGCGTTC 
ATTGCCGGAA TCGGGGCAGC CTGCTGGATC ATCCTCATGG TGTTTAGCAT CTGGCTTTAT 
CGGCACCGCA AGAAGAGAAA CGGGCTGACT AGTACTTACG CGGGTATCAG AAAAGTCCCG 
TCTTTTACCT TCACACCAAC AGTAACTTAT CAGAGAGGCG GTGAAGCTGT AAGCAGTGGA 
GGGAGGCCAG GACTTCTGAA CATCGGTGAA CCTGCCACAC AGCCATGGCT GGCCGACACA 
TGGCCTAATA CTGGCAACAG TCACAATGAC TGCTCCATCA CTTGCTGCAC TGCAGCCAAT 
GGTAACAGTG ATAGCAACCT GACCACCTAC AGTCGCCCAG CTGATTGTAT AGCAAATTAT 
AACAACCAAC TGGATAACAA ACAAACAAAT CTGATGCTCC CTGAGTCAAC TGTTTATGGT 
GATGTGGACC TTAGTAACAA AATCAATGAG ATGAAAACCT TCAATAGCCC AAATCTGAAG 
GACGGGCGTT TTGTCAATCC ATCAGGGCAG CCCACTCCTT ACGCCACCAC CCAGCTCATC 
CAGTCCAACC TCAGCAACAA CATGAACAAT GGCAGTGGGG ACCCCAGCGA GAAGCACTGG 
AAGCCACCTG GACAGCAGAA ACAGGAAGTG GCACCAATTC AGTATAACAT CATGGAGCAA 
AACAAACTGA ACAAAGATTA TCGAGCAAAT GACACAATTC CTCCAACTAT CCCATACAAC 
CAGTCATACG ACCAGAATAC AGGAGGATCT TACAACAGTT CAGACCGGGG CAGTAGTACA 
TCTGGGAGTC AAGGGCACAA GAAAGGGGCA CGGACACCCA AAGTTCCAAA ACAGGGTGGC 
ATGAACTGGG CAGACCTGCT TCCTCCTCCC CCAGCACATC CTCCGCCTCA CAGCAATAGT 
GAAGAGTACA GCATTTCTGT AGATGAAAGT TATGACCAAG AAATGCCGTG TCCTGTGCCA 
CCAGCCAGGA TGTATTTGCA ACAGGACGAA CTGGAAGAGG AGGAAGATGA ACGAGGCCCC 
ACCCCCCCTG TACGGGGAGC AGCTTCTTCT CCAGCTGCTG TGTCTTACAG CCATCAGTCC 
ACTGCGACAC TGACCCCGTC TCCACAAGAA GAACTCCAGC CCATGTTACA GGATTGCCCA 
GAGGAGATTG GCCACATGCA GCACCAACCC GATAGGAGAC GGCAGCCTGT GAGTCCCCCT 
CCGCCACCAC GACCGATTTC CCCACCACAT ACCTATGGCT ACATTTCAGG ACCTCTTGTC 
TCAGATATGG ATACGGATGC TCCAGAAGAG GAAGAAGATG AAGCAGACAT GGAAGTGGCC 
AAGATGCAGA CCAGACGGCT TTTGTTACGT GGGCTTGAGC AGACCCCTGC CTCCAGTGTA 
GGGGACCTCG AGAGCTCTGT CACTGGGTCC ATGATCAATG GCTGGGGCTC AGCCTCAGAG 
GAGGACAACA TTTCCAGTGG ACGCTCCAGT GTTAGTTCTT CCGATGGCTC CTTCTTCACC 
GATGCCGATT TTGCCCAGGC AGTGGCGGCA GCAGCAGAGT ATGCTGGCCT CAAAGTGGCA 
CGTCGCCAAA TGCAGGATGC CGCTGGCCGC CGACATTTTC ATGCATCCCA GTGCCCAAGA 
CCCACAAGTC CTGTTTCTAC AGACAGCAAC ATGAGTGCTG CGGTAATACA GAAGGCCAGA 
CCAGCCAAGA AGCAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGCCTA CACAGATGAT 
CTTCCACCTC CTCCAGTGCC ACCACCTGCT ATAAAGTCAC CCACTGTTCA ATCCAAGGCA 
CAGCTGGAGG TACGACCTGT AATGGCACCC AAACTACCTT CTATAGAAGC AAGAACAGAC 
AGGTCATCAG ATAGAAAAGT AGGCAGCTAC AAGGGGAGAG AAGTATTGGA TGGAAGACAA 
GTTGCAGATA TGAGAACAAA TCCAGGTGAT ACCAGAGAAG CACAGGAGCA GCAAAATGAT 
GGGAAAGGAC GAGGAAACAA GGCAGCAAAA CGCGACCTTC CACCAGCAAA GACTCATCTC 
ATCCAAGAGG ATATTCTACC TTACTGTAGA CCTACTTTTC CGACATCAAA TAATCCCAGA 
GATCCTAGTT CTTCAAGCTC AATGTCATCA AGAGGATCAG GAAGCAGACA AAGAGAACAA 
GCAAATGTAG GTCGAAGAAA TATTGCAGAA ATGCAGGTTC TTGGAGGATA TGAAAGAGGA 
GAAGATAATA ATGAAGAATT AGAGGAAACT GAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839549.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695108.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OFb48393
Clone ID Related Accession (Same CDS sequence)	XM_019839549.1
Accession Version	XM_019839549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGGCGA ACTGTAACTT TTCAGTGTGA AGCAACTGGA 
AATCCTCAAC CAGCTATTTT TTGGAGACGA GAGGGGAGTC AGAATTTGCT TTTCTCATAT 
CAGCCACCAC AGTCAACCAG CCGTTTTTCA GTCTCCCAGA CTGGTGATCT CACTATCACT 
AACGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTAACT GATGTGATTG CAGACCGGCC TCCCCCGGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTG GCCGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACGATT CTTTGGAGAA AGGATGGAGT GCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACAGGAGTGC TGCAGATCCG ATATGCTAAG 
CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA GGCAACATGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACTGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACG 
GTAACCTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTACATTATA 
GAAGCCTTCA GCCACGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACG 
GAAACATTCG CCATTAAAGG ACTAAAACCT AATGCAATTT ACCTTTTCCT TGTGAGAGCA 
GCAAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACGCAAGAT 
GTTCCCCCAA CGAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAATGTT 
GTACTGCACC TGCACAACCC CACCATCCTT TCTTCCTCTT CAATCGAAGT ACACTGGACA 
GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCAGGAGCC 
AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG 
GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAG TTCGCAAAGA CCTTAGAAGA AGCACCCAGT 
GCCCCACCCC AAAGTGTAAC TGTGTCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGG CGCCTCCAGA AGACACTCAA AACGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTAG ATATCATATA AACAAGACAG TAGATGGTTC CACTTTTTCT 
GTGGTCATCC CTTCTCTGGT TCCTGGGATC CGGTACAGCA TAGAGGTGGC AGCCAGCACT 
GGGGCCGGGG CCGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC 
CCTGTGTCGC CTGAAGACCA GGTCAGCCTT GCTCAGCAGA TCTCCGATGT GGTGAAGCAG 
CCAGCGTTCA TTGCCGGAAT CGGGGCAGCC TGCTGGATCA TCCTCATGGT GTTTAGCATC 
TGGCTTTATC GGCACCGCAA GAAGAGAAAC GGGCTGACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG CGGTGAAGCT GTAAGCAGTG GAGGGAGGCC AGGACTTCTG 
AACATCGGTG AACCTGCCAC ACAGCCATGG CTGGCCGACA CATGGCCTAA TACTGGCAAC 
AGTCACAATG ACTGCTCCAT CACTTGCTGC ACTGCAGCCA ATGGTAACAG TGATAGCAAC 
CTGACCACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGACGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCCACTCC TTACGCCACC ACCCAGCTCA TCCAGTCCAA CCTCAGCAAC 
AACATGAACA ATGGCAGTGG GGACCCCAGC GAGAAGCACT GGAAGCCACC TGGACAGCAG 
AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT 
TATCGAGCAA ATGACACAAT TCCTCCAACT ATCCCATACA ACCAGTCATA CGACCAGAAT 
ACAGGAGGAT CTTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC 
AAGAAAGGGG CACGGACACC CAAAGTTCCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCGCCT CACAGCAATA GTGAAGAGTA CAGCATTTCT 
GTAGATGAAA GTTATGACCA AGAAATGCCG TGTCCTGTGC CACCAGCCAG GATGTATTTG 
CAACAGGACG AACTGGAAGA GGAGGAAGAT GAACGAGGCC CCACCCCCCC TGTACGGGGA 
GCAGCTTCTT CTCCAGCTGC TGTGTCTTAC AGCCATCAGT CCACTGCGAC ACTGACCCCG 
TCTCCACAAG AAGAACTCCA GCCCATGTTA CAGGATTGCC CAGAGGAGAT TGGCCACATG 
CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCCC CTCCGCCACC ACGACCGATT 
TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACGGAT 
GCTCCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG 
CTTTTGTTAC GTGGGCTTGA GCAGACCCCT GCCTCCAGTG TAGGGGACCT CGAGAGCTCT 
GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT 
GGACGCTCCA GTGTTAGTTC TTCCGATGGC TCCTTCTTCA CCGATGCCGA TTTTGCCCAG 
GCAGTGGCGG CAGCAGCAGA GTATGCTGGC CTCAAAGTGG CACGTCGCCA AATGCAGGAT 
GCCGCTGGCC GCCGACATTT TCATGCATCC CAGTGCCCAA GACCCACAAG TCCTGTTTCT 
ACAGACAGCA ACATGAGTGC TGCGGTAATA CAGAAGGCCA GACCAGCCAA GAAGCAAAAA 
CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCTCCAGTG 
CCACCACCTG CTATAAAGTC ACCCACTGTT CAATCCAAGG CACAGCTGGA GGTACGACCT 
GTAATGGCAC CCAAACTACC TTCTATAGAA GCAAGAACAG ACAGGTCATC AGATAGAAAA 
GTAGGCAGCT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCAGA TATGAGAACA 
AATCCAGGTG ATACCAGAGA AGCACAGGAG CAGCAAAATG ATGGGAAAGG ACGAGGAAAC 
AAGGCAGCAA AACGCGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695108.1
CDS	1041..5861
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839549.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839549.1

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGGCGA ACTGTAACTT TTCAGTGTGA AGCAACTGGA 
AATCCTCAAC CAGCTATTTT TTGGAGACGA GAGGGGAGTC AGAATTTGCT TTTCTCATAT 
CAGCCACCAC AGTCAACCAG CCGTTTTTCA GTCTCCCAGA CTGGTGATCT CACTATCACT 
AACGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTAACT GATGTGATTG CAGACCGGCC TCCCCCGGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTG GCCGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACGATT CTTTGGAGAA AGGATGGAGT GCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACAGGAGTGC TGCAGATCCG ATATGCTAAG 
CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA GGCAACATGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACTGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACG 
GTAACCTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTACATTATA 
GAAGCCTTCA GCCACGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACG 
GAAACATTCG CCATTAAAGG ACTAAAACCT AATGCAATTT ACCTTTTCCT TGTGAGAGCA 
GCAAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACGCAAGAT 
GTTCCCCCAA CGAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAATGTT 
GTACTGCACC TGCACAACCC CACCATCCTT TCTTCCTCTT CAATCGAAGT ACACTGGACA 
GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCAGGAGCC 
AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG 
GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAG TTCGCAAAGA CCTTAGAAGA AGCACCCAGT 
GCCCCACCCC AAAGTGTAAC TGTGTCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGG CGCCTCCAGA AGACACTCAA AACGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTAG ATATCATATA AACAAGACAG TAGATGGTTC CACTTTTTCT 
GTGGTCATCC CTTCTCTGGT TCCTGGGATC CGGTACAGCA TAGAGGTGGC AGCCAGCACT 
GGGGCCGGGG CCGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC 
CCTGTGTCGC CTGAAGACCA GGTCAGCCTT GCTCAGCAGA TCTCCGATGT GGTGAAGCAG 
CCAGCGTTCA TTGCCGGAAT CGGGGCAGCC TGCTGGATCA TCCTCATGGT GTTTAGCATC 
TGGCTTTATC GGCACCGCAA GAAGAGAAAC GGGCTGACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG CGGTGAAGCT GTAAGCAGTG GAGGGAGGCC AGGACTTCTG 
AACATCGGTG AACCTGCCAC ACAGCCATGG CTGGCCGACA CATGGCCTAA TACTGGCAAC 
AGTCACAATG ACTGCTCCAT CACTTGCTGC ACTGCAGCCA ATGGTAACAG TGATAGCAAC 
CTGACCACCT ACAGTCGCCC AGCTGATTGT ATAGCAAATT ATAACAACCA ACTGGATAAC 
AAACAAACAA ATCTGATGCT CCCTGAGTCA ACTGTTTATG GTGATGTGGA CCTTAGTAAC 
AAAATCAATG AGATGAAAAC CTTCAATAGC CCAAATCTGA AGGACGGGCG TTTTGTCAAT 
CCATCAGGGC AGCCCACTCC TTACGCCACC ACCCAGCTCA TCCAGTCCAA CCTCAGCAAC 
AACATGAACA ATGGCAGTGG GGACCCCAGC GAGAAGCACT GGAAGCCACC TGGACAGCAG 
AAACAGGAAG TGGCACCAAT TCAGTATAAC ATCATGGAGC AAAACAAACT GAACAAAGAT 
TATCGAGCAA ATGACACAAT TCCTCCAACT ATCCCATACA ACCAGTCATA CGACCAGAAT 
ACAGGAGGAT CTTACAACAG TTCAGACCGG GGCAGTAGTA CATCTGGGAG TCAAGGGCAC 
AAGAAAGGGG CACGGACACC CAAAGTTCCA AAACAGGGTG GCATGAACTG GGCAGACCTG 
CTTCCTCCTC CCCCAGCACA TCCTCCGCCT CACAGCAATA GTGAAGAGTA CAGCATTTCT 
GTAGATGAAA GTTATGACCA AGAAATGCCG TGTCCTGTGC CACCAGCCAG GATGTATTTG 
CAACAGGACG AACTGGAAGA GGAGGAAGAT GAACGAGGCC CCACCCCCCC TGTACGGGGA 
GCAGCTTCTT CTCCAGCTGC TGTGTCTTAC AGCCATCAGT CCACTGCGAC ACTGACCCCG 
TCTCCACAAG AAGAACTCCA GCCCATGTTA CAGGATTGCC CAGAGGAGAT TGGCCACATG 
CAGCACCAAC CCGATAGGAG ACGGCAGCCT GTGAGTCCCC CTCCGCCACC ACGACCGATT 
TCCCCACCAC ATACCTATGG CTACATTTCA GGACCTCTTG TCTCAGATAT GGATACGGAT 
GCTCCAGAAG AGGAAGAAGA TGAAGCAGAC ATGGAAGTGG CCAAGATGCA GACCAGACGG 
CTTTTGTTAC GTGGGCTTGA GCAGACCCCT GCCTCCAGTG TAGGGGACCT CGAGAGCTCT 
GTCACTGGGT CCATGATCAA TGGCTGGGGC TCAGCCTCAG AGGAGGACAA CATTTCCAGT 
GGACGCTCCA GTGTTAGTTC TTCCGATGGC TCCTTCTTCA CCGATGCCGA TTTTGCCCAG 
GCAGTGGCGG CAGCAGCAGA GTATGCTGGC CTCAAAGTGG CACGTCGCCA AATGCAGGAT 
GCCGCTGGCC GCCGACATTT TCATGCATCC CAGTGCCCAA GACCCACAAG TCCTGTTTCT 
ACAGACAGCA ACATGAGTGC TGCGGTAATA CAGAAGGCCA GACCAGCCAA GAAGCAAAAA 
CACCAGCCAG GACATCTGCG CAGAGAAGCC TACACAGATG ATCTTCCACC TCCTCCAGTG 
CCACCACCTG CTATAAAGTC ACCCACTGTT CAATCCAAGG CACAGCTGGA GGTACGACCT 
GTAATGGCAC CCAAACTACC TTCTATAGAA GCAAGAACAG ACAGGTCATC AGATAGAAAA 
GTAGGCAGCT ACAAGGGGAG AGAAGTATTG GATGGAAGAC AAGTTGCAGA TATGAGAACA 
AATCCAGGTG ATACCAGAGA AGCACAGGAG CAGCAAAATG ATGGGAAAGG ACGAGGAAAC 
AAGGCAGCAA AACGCGACCT TCCACCAGCA AAGACTCATC TCATCCAAGA GGATATTCTA 
CCTTACTGTA GACCTACTTT TCCGACATCA AATAATCCCA GAGATCCTAG TTCTTCAAGC 
TCAATGTCAT CAAGAGGATC AGGAAGCAGA CAAAGAGAAC AAGCAAATGT AGGTCGAAGA 
AATATTGCAG AAATGCAGGT TCTTGGAGGA TATGAAAGAG GAGAAGATAA TAATGAAGAA 
TTAGAGGAAA CTGAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839550.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695109.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OFb48394
Clone ID Related Accession (Same CDS sequence)	XM_019839550.1
Accession Version	XM_019839550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGGCGAACT GTAACTTTTC AGTGTGAAGC AACTGGAAAT 
CCTCAACCAG CTATTTTTTG GAGACGAGAG GGGAGTCAGA ATTTGCTTTT CTCATATCAG 
CCACCACAGT CAACCAGCCG TTTTTCAGTC TCCCAGACTG GTGATCTCAC TATCACTAAC 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC TGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTAACTGAT GTGATTGCAG ACCGGCCTCC CCCGGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTGGCC GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGCACC CACGATTCTT TGGAGAAAGG ATGGAGTGCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACA GGAGTGCTGC AGATCCGATA TGCTAAGCTG 
GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAGGC AACATGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACTGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACGGTA 
ACCTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA CATTATAGAA 
GCCTTCAGCC ACGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACGGAA 
ACATTCGCCA TTAAAGGACT AAAACCTAAT GCAATTTACC TTTTCCTTGT GAGAGCAGCA 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC GCAAGATGTT 
CCCCCAACGA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAATGTTGTA 
CTGCACCTGC ACAACCCCAC CATCCTTTCT TCCTCTTCAA TCGAAGTACA CTGGACAGTG 
GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC AGGAGCCAAC 
CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTT 
ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAGTTC GCAAAGACCT TAGAAGAAGC ACCCAGTGCC 
CCACCCCAAA GTGTAACTGT GTCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGGCGC CTCCAGAAGA CACTCAAAAC GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTAGATA TCATATAAAC AAGACAGTAG ATGGTTCCAC TTTTTCTGTG 
GTCATCCCTT CTCTGGTTCC TGGGATCCGG TACAGCATAG AGGTGGCAGC CAGCACTGGG 
GCCGGGGCCG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCT 
GTGTCGCCTG AAGACCAGGT CAGCCTTGCT CAGCAGATCT CCGATGTGGT GAAGCAGCCA 
GCGTTCATTG CCGGAATCGG GGCAGCCTGC TGGATCATCC TCATGGTGTT TAGCATCTGG 
CTTTATCGGC ACCGCAAGAA GAGAAACGGG CTGACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGCGGTGA AGCTGTAAGC 
AGTGGAGGGA GGCCAGGACT TCTGAACATC GGTGAACCTG CCACACAGCC ATGGCTGGCC 
GACACATGGC CTAATACTGG CAACAGTCAC AATGACTGCT CCATCACTTG CTGCACTGCA 
GCCAATGGTA ACAGTGATAG CAACCTGACC ACCTACAGTC GCCCAGGGCA GCCCACTCCT 
TACGCCACCA CCCAGCTCAT CCAGTCCAAC CTCAGCAACA ACATGAACAA TGGCAGTGGG 
GACCCCAGCG AGAAGCACTG GAAGCCACCT GGACAGCAGA AACAGGAAGT GGCACCAATT 
CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGAGCAAA TGACACAATT 
CCTCCAACTA TCCCATACAA CCAGTCATAC GACCAGAATA CAGGAGGATC TTACAACAGT 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAAGGGCACA AGAAAGGGGC ACGGACACCC 
AAAGTTCCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCGCCTC ACAGCAATAG TGAAGAGTAC AGCATTTCTG TAGATGAAAG TTATGACCAA 
GAAATGCCGT GTCCTGTGCC ACCAGCCAGG ATGTATTTGC AACAGGACGA ACTGGAAGAG 
GAGGAAGATG AACGAGGCCC CACCCCCCCT GTACGGGGAG CAGCTTCTTC TCCAGCTGCT 
GTGTCTTACA GCCATCAGTC CACTGCGACA CTGACCCCGT CTCCACAAGA AGAACTCCAG 
CCCATGTTAC AGGATTGCCC AGAGGAGATT GGCCACATGC AGCACCAACC CGATAGGAGA 
CGGCAGCCTG TGAGTCCCCC TCCGCCACCA CGACCGATTT CCCCACCACA TACCTATGGC 
TACATTTCAG GACCTCTTGT CTCAGATATG GATACGGATG CTCCAGAAGA GGAAGAAGAT 
GAAGCAGACA TGGAAGTGGC CAAGATGCAG ACCAGACGGC TTTTGTTACG TGGGCTTGAG 
CAGACCCCTG CCTCCAGTGT AGGGGACCTC GAGAGCTCTG TCACTGGGTC CATGATCAAT 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGTG GACGCTCCAG TGTTAGTTCT 
TCCGATGGCT CCTTCTTCAC CGATGCCGAT TTTGCCCAGG CAGTGGCGGC AGCAGCAGAG 
TATGCTGGCC TCAAAGTGGC ACGTCGCCAA ATGCAGGATG CCGCTGGCCG CCGACATTTT 
CATGCATCCC AGTGCCCAAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CATGAGTGCT 
GCGGTAATAC AGAAGGCCAG ACCAGCCAAG AAGCAAAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAGCCT ACACAGATGA TCTTCCACCT CCTCCAGTGC CACCACCTGC TATAAAGTCA 
CCCACTGTTC AATCCAAGGC ACAGCTGGAG GTACGACCTG TAATGGCACC CAAACTACCT 
TCTATAGAAG CAAGAACAGA CAGGTCATCA GATAGAAAAG TAGGCAGCTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA AGTTGCAGAT ATGAGAACAA ATCCAGGTGA TACCAGAGAA 
GCACAGGAGC AGCAAAATGA TGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGCGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTACTGTAG ACCTACTTTT 
CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTT 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695109.1
CDS	146..4945
Translation	MKWKHVPFLVIISLLSLTEDHLFLAQLIPDPEDLERGNDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839550.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839550.1

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGTTGGG TCTGAACCTC CACATTTTGT TGTGAAACCC 
CGTGACCAGG TTGTTGCTTT GGGGCGAACT GTAACTTTTC AGTGTGAAGC AACTGGAAAT 
CCTCAACCAG CTATTTTTTG GAGACGAGAG GGGAGTCAGA ATTTGCTTTT CTCATATCAG 
CCACCACAGT CAACCAGCCG TTTTTCAGTC TCCCAGACTG GTGATCTCAC TATCACTAAC 
GTCCAGCGAT CTGATGTTGG TTATTATATC TGCCAGACTT TAAATGTTGC TGGAAGTATC 
ATCACAAAAG CATATTTGGA AGTAACTGAT GTGATTGCAG ACCGGCCTCC CCCGGTCATT 
CGACAAGGTC CTGTGAATCA GACTGTGGCC GTGGATGGTA CTTTAGTGCT CAGCTGTGTG 
GCCACAGGCA GTCCAGCACC CACGATTCTT TGGAGAAAGG ATGGAGTGCT TGTTTCAACC 
CAAGATTCTC GAATCAAACA GCTGGAGACA GGAGTGCTGC AGATCCGATA TGCTAAGCTG 
GGTGACACAG GTCGGTACAC CTGCATTGCA TCAACCCCCA GTGGTGAGGC AACATGGAGT 
GCTTACATTG AAGTCCAAGA ATTTGGAGTT CCAGTTCAGC CTCCAAGACC CACTGACCCA 
AATTTAATCC CTAGTGCCCC CTCAAAACCT GAAGTTACAG ATGTCAGCAG AAATACGGTA 
ACCTTGTCAT GGCAACCAAA TTTGAATTCA GGTGCAACTC CAACATCTTA CATTATAGAA 
GCCTTCAGCC ACGCATCTGG TAGCAGCTGG CAGACCGTAG CAGAGAATGT GAAAACGGAA 
ACATTCGCCA TTAAAGGACT AAAACCTAAT GCAATTTACC TTTTCCTTGT GAGAGCAGCA 
AATGCATATG GAATTAGTGA TCCAAGCCAG ATATCAGATC CAGTGAAAAC GCAAGATGTT 
CCCCCAACGA GTCAGGGAGT AGATCACAAG CAGGTCCAGA GAGAATTGGG AAATGTTGTA 
CTGCACCTGC ACAACCCCAC CATCCTTTCT TCCTCTTCAA TCGAAGTACA CTGGACAGTG 
GACCAACAAT CTCAGTATAT TCAAGGATAT AAAATTCTTT ATCGGCCATC AGGAGCCAAC 
CATGGTGAAT CGGAGTGGTT AGTTTTTGAA GTGAGGACCC CAACCAAAAA TAGTGTGGTT 
ATCCCTGATC TCAAAAAGGG AGTCAACTAT GAAATTAAGG CTCGTCCTTT TTTTAATGAA 
TTTCAAGGAG CAGATAGTGA AATTAAGTTC GCAAAGACCT TAGAAGAAGC ACCCAGTGCC 
CCACCCCAAA GTGTAACTGT GTCAAAGAAT GATGGAAATG GAACTGCAAT TCTTGTTAGT 
TGGCAGGCGC CTCCAGAAGA CACTCAAAAC GGAATGGTCC AAGAGTATAA GGTTTGGTGT 
CTGGGCAATG AAACTAGATA TCATATAAAC AAGACAGTAG ATGGTTCCAC TTTTTCTGTG 
GTCATCCCTT CTCTGGTTCC TGGGATCCGG TACAGCATAG AGGTGGCAGC CAGCACTGGG 
GCCGGGGCCG GGGTGAAGAG CGAGCCACAG TTCATCCAGT TAGATTCTCA TGGAAACCCT 
GTGTCGCCTG AAGACCAGGT CAGCCTTGCT CAGCAGATCT CCGATGTGGT GAAGCAGCCA 
GCGTTCATTG CCGGAATCGG GGCAGCCTGC TGGATCATCC TCATGGTGTT TAGCATCTGG 
CTTTATCGGC ACCGCAAGAA GAGAAACGGG CTGACTAGTA CTTACGCGGG TATCAGAAAA 
GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACTTATCAGA GAGGCGGTGA AGCTGTAAGC 
AGTGGAGGGA GGCCAGGACT TCTGAACATC GGTGAACCTG CCACACAGCC ATGGCTGGCC 
GACACATGGC CTAATACTGG CAACAGTCAC AATGACTGCT CCATCACTTG CTGCACTGCA 
GCCAATGGTA ACAGTGATAG CAACCTGACC ACCTACAGTC GCCCAGGGCA GCCCACTCCT 
TACGCCACCA CCCAGCTCAT CCAGTCCAAC CTCAGCAACA ACATGAACAA TGGCAGTGGG 
GACCCCAGCG AGAAGCACTG GAAGCCACCT GGACAGCAGA AACAGGAAGT GGCACCAATT 
CAGTATAACA TCATGGAGCA AAACAAACTG AACAAAGATT ATCGAGCAAA TGACACAATT 
CCTCCAACTA TCCCATACAA CCAGTCATAC GACCAGAATA CAGGAGGATC TTACAACAGT 
TCAGACCGGG GCAGTAGTAC ATCTGGGAGT CAAGGGCACA AGAAAGGGGC ACGGACACCC 
AAAGTTCCAA AACAGGGTGG CATGAACTGG GCAGACCTGC TTCCTCCTCC CCCAGCACAT 
CCTCCGCCTC ACAGCAATAG TGAAGAGTAC AGCATTTCTG TAGATGAAAG TTATGACCAA 
GAAATGCCGT GTCCTGTGCC ACCAGCCAGG ATGTATTTGC AACAGGACGA ACTGGAAGAG 
GAGGAAGATG AACGAGGCCC CACCCCCCCT GTACGGGGAG CAGCTTCTTC TCCAGCTGCT 
GTGTCTTACA GCCATCAGTC CACTGCGACA CTGACCCCGT CTCCACAAGA AGAACTCCAG 
CCCATGTTAC AGGATTGCCC AGAGGAGATT GGCCACATGC AGCACCAACC CGATAGGAGA 
CGGCAGCCTG TGAGTCCCCC TCCGCCACCA CGACCGATTT CCCCACCACA TACCTATGGC 
TACATTTCAG GACCTCTTGT CTCAGATATG GATACGGATG CTCCAGAAGA GGAAGAAGAT 
GAAGCAGACA TGGAAGTGGC CAAGATGCAG ACCAGACGGC TTTTGTTACG TGGGCTTGAG 
CAGACCCCTG CCTCCAGTGT AGGGGACCTC GAGAGCTCTG TCACTGGGTC CATGATCAAT 
GGCTGGGGCT CAGCCTCAGA GGAGGACAAC ATTTCCAGTG GACGCTCCAG TGTTAGTTCT 
TCCGATGGCT CCTTCTTCAC CGATGCCGAT TTTGCCCAGG CAGTGGCGGC AGCAGCAGAG 
TATGCTGGCC TCAAAGTGGC ACGTCGCCAA ATGCAGGATG CCGCTGGCCG CCGACATTTT 
CATGCATCCC AGTGCCCAAG ACCCACAAGT CCTGTTTCTA CAGACAGCAA CATGAGTGCT 
GCGGTAATAC AGAAGGCCAG ACCAGCCAAG AAGCAAAAAC ACCAGCCAGG ACATCTGCGC 
AGAGAAGCCT ACACAGATGA TCTTCCACCT CCTCCAGTGC CACCACCTGC TATAAAGTCA 
CCCACTGTTC AATCCAAGGC ACAGCTGGAG GTACGACCTG TAATGGCACC CAAACTACCT 
TCTATAGAAG CAAGAACAGA CAGGTCATCA GATAGAAAAG TAGGCAGCTA CAAGGGGAGA 
GAAGTATTGG ATGGAAGACA AGTTGCAGAT ATGAGAACAA ATCCAGGTGA TACCAGAGAA 
GCACAGGAGC AGCAAAATGA TGGGAAAGGA CGAGGAAACA AGGCAGCAAA ACGCGACCTT 
CCACCAGCAA AGACTCATCT CATCCAAGAG GATATTCTAC CTTACTGTAG ACCTACTTTT 
CCGACATCAA ATAATCCCAG AGATCCTAGT TCTTCAAGCT CAATGTCATC AAGAGGATCA 
GGAAGCAGAC AAAGAGAACA AGCAAATGTA GGTCGAAGAA ATATTGCAGA AATGCAGGTT 
CTTGGAGGAT ATGAAAGAGG AGAAGATAAT AATGAAGAAT TAGAGGAAAC TGAAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839551.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695110.1 Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

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CloneID	OFb48395
Clone ID Related Accession (Same CDS sequence)	XM_019839551.1
Accession Version	XM_019839551.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	roundabout homolog 1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGGCGA ACTGTAACTT TTCAGTGTGA AGCAACTGGA 
AATCCTCAAC CAGCTATTTT TTGGAGACGA GAGGGGAGTC AGAATTTGCT TTTCTCATAT 
CAGCCACCAC AGTCAACCAG CCGTTTTTCA GTCTCCCAGA CTGGTGATCT CACTATCACT 
AACGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTAACT GATGTGATTG CAGACCGGCC TCCCCCGGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTG GCCGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACGATT CTTTGGAGAA AGGATGGAGT GCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACAGGAGTGC TGCAGATCCG ATATGCTAAG 
CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA GGCAACATGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACTGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACG 
GTAACCTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTACATTATA 
GAAGCCTTCA GCCACGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACG 
GAAACATTCG CCATTAAAGG ACTAAAACCT AATGCAATTT ACCTTTTCCT TGTGAGAGCA 
GCAAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACGCAAGAT 
GTTCCCCCAA CGAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAATGTT 
GTACTGCACC TGCACAACCC CACCATCCTT TCTTCCTCTT CAATCGAAGT ACACTGGACA 
GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCAGGAGCC 
AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG 
GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAG TTCGCAAAGA CCTTAGAAGA AGCACCCAGT 
GCCCCACCCC AAAGTGTAAC TGTGTCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGG CGCCTCCAGA AGACACTCAA AACGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTAG ATATCATATA AACAAGACAG TAGATGGTTC CACTTTTTCT 
GTGGTCATCC CTTCTCTGGT TCCTGGGATC CGGTACAGCA TAGAGGTGGC AGCCAGCACT 
GGGGCCGGGG CCGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC 
CCTGTGTCGC CTGAAGACCA GGTCAGCCTT GCTCAGCAGA TCTCCGATGT GGTGAAGCAG 
CCAGCGTTCA TTGCCGGAAT CGGGGCAGCC TGCTGGATCA TCCTCATGGT GTTTAGCATC 
TGGCTTTATC GGCACCGCAA GAAGAGAAAC GGGCTGACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG CGGTGAAGCT GTAAGCAGTG GAGGGAGGCC AGGACTTCTG 
AACATCGGTG AACCTGCCAC ACAGCCATGG CTGGCCGACA CATGGCCTAA TACTGGCAAC 
AGTCACAATG ACTGCTCCAT CACTTGCTGC ACTGCAGCCA ATGGTAACAG TGATAGCAAC 
CTGACCACCT ACAGTCGCCC AGGGCAGCCC ACTCCTTACG CCACCACCCA GCTCATCCAG 
TCCAACCTCA GCAACAACAT GAACAATGGC AGTGGGGACC CCAGCGAGAA GCACTGGAAG 
CCACCTGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG AGCAAATGAC ACAATTCCTC CAACTATCCC ATACAACCAG 
TCATACGACC AGAATACAGG AGGATCTTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TTCCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CGCCTCACAG CAATAGTGAA 
GAGTACAGCA TTTCTGTAGA TGAAAGTTAT GACCAAGAAA TGCCGTGTCC TGTGCCACCA 
GCCAGGATGT ATTTGCAACA GGACGAACTG GAAGAGGAGG AAGATGAACG AGGCCCCACC 
CCCCCTGTAC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTACAGCCA TCAGTCCACT 
GCGACACTGA CCCCGTCTCC ACAAGAAGAA CTCCAGCCCA TGTTACAGGA TTGCCCAGAG 
GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCCCCTCCG 
CCACCACGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA 
GATATGGATA CGGATGCTCC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG 
ATGCAGACCA GACGGCTTTT GTTACGTGGG CTTGAGCAGA CCCCTGCCTC CAGTGTAGGG 
GACCTCGAGA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGTGGACG CTCCAGTGTT AGTTCTTCCG ATGGCTCCTT CTTCACCGAT 
GCCGATTTTG CCCAGGCAGT GGCGGCAGCA GCAGAGTATG CTGGCCTCAA AGTGGCACGT 
CGCCAAATGC AGGATGCCGC TGGCCGCCGA CATTTTCATG CATCCCAGTG CCCAAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACATG AGTGCTGCGG TAATACAGAA GGCCAGACCA 
GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCTC CAGTGCCACC ACCTGCTATA AAGTCACCCA CTGTTCAATC CAAGGCACAG 
CTGGAGGTAC GACCTGTAAT GGCACCCAAA CTACCTTCTA TAGAAGCAAG AACAGACAGG 
TCATCAGATA GAAAAGTAGG CAGCTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCAGATATGA GAACAAATCC AGGTGATACC AGAGAAGCAC AGGAGCAGCA AAATGATGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGC GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTTCTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695110.1
CDS	1042..5697
Translation	MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVPPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839551.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839551.1

ORF Insert Sequence:

ATGATCGCGG AGCCTGCTCA CTTTTACCTC TTTGGATTAA TATGTCTCTG TTCAGGCTCC 
CGTCTTCGTC AGGAAGATTT TCCACCTCGC ATTGTTGAAC ACCCTTCGGA CCTAATTGTG 
TCAAAAGGAG AACCTGCAAC TTTGAACTGT AAAGCTGAAG GCCGCCCCAC ACCCACTATC 
GAATGGTACA AAGGGGGGGA GAGAGTGGAG ACGGACAAAG ATGACCCTCG ATCACACCGA 
ATGTTGCTGC CAAGTGGATC TTTATTTTTC TTACGTATAG TACATGGACG AAAAAGCAGG 
CCTGATGAAG GGGTCTACGT CTGTGTAGCA AGGAATTACC TCGGAGAGGC CGTGAGCCAT 
AATGCATCGC TGGAGGTGGC TATACTCCGG GATGACTTCA GACAAAATCC TTCCGATGTC 
ATGGTGGCAG TGGGGGAGCC TGCAGTGATG GAATGCCAGC CTCCACGAGG CCATCCTGAA 
CCCACCATTT CATGGAAGAA AGATGGCTCT CCGCTGGATG ACAAAGATGA ACGAATAACT 
ATTCGAGGAG GAAAGCTCAT GATCACTTAC ACCCGCAAAA GTGATGCTGG CAAATACGTT 
TGTGTTGGTA CCAACATGGT TGGGGAACGG GAGAGTGAAG TGGCTGAGCT AACGGTTTTG 
GAGAGACCAT CGTTTGTAAA GAGACCTAGT AACTTGGCGG TAACTGTGGA CGACAGTGCG 
GAATTTAAAT GTGAGGCCCG AGGTGACCCT GTGCCTACGG TACGATGGAG GAAAGATGAT 
GGAGAACTGC CCAAATCCAG ATATGAAATC AGAGATGATC ATACCTTGAA AATTCGGAAG 
GTGATGGCTG GTGACATGGG GTCATATACT TGTGTTGCGG AAAATATGGT TGGAAAAGCG 
GAAGCATCAG CTACTCTGAC TGTCCAAGTT GGGTCTGAAC CTCCACATTT TGTTGTGAAA 
CCCCGTGACC AGGTTGTTGC TTTGGGGCGA ACTGTAACTT TTCAGTGTGA AGCAACTGGA 
AATCCTCAAC CAGCTATTTT TTGGAGACGA GAGGGGAGTC AGAATTTGCT TTTCTCATAT 
CAGCCACCAC AGTCAACCAG CCGTTTTTCA GTCTCCCAGA CTGGTGATCT CACTATCACT 
AACGTCCAGC GATCTGATGT TGGTTATTAT ATCTGCCAGA CTTTAAATGT TGCTGGAAGT 
ATCATCACAA AAGCATATTT GGAAGTAACT GATGTGATTG CAGACCGGCC TCCCCCGGTC 
ATTCGACAAG GTCCTGTGAA TCAGACTGTG GCCGTGGATG GTACTTTAGT GCTCAGCTGT 
GTGGCCACAG GCAGTCCAGC ACCCACGATT CTTTGGAGAA AGGATGGAGT GCTTGTTTCA 
ACCCAAGATT CTCGAATCAA ACAGCTGGAG ACAGGAGTGC TGCAGATCCG ATATGCTAAG 
CTGGGTGACA CAGGTCGGTA CACCTGCATT GCATCAACCC CCAGTGGTGA GGCAACATGG 
AGTGCTTACA TTGAAGTCCA AGAATTTGGA GTTCCAGTTC AGCCTCCAAG ACCCACTGAC 
CCAAATTTAA TCCCTAGTGC CCCCTCAAAA CCTGAAGTTA CAGATGTCAG CAGAAATACG 
GTAACCTTGT CATGGCAACC AAATTTGAAT TCAGGTGCAA CTCCAACATC TTACATTATA 
GAAGCCTTCA GCCACGCATC TGGTAGCAGC TGGCAGACCG TAGCAGAGAA TGTGAAAACG 
GAAACATTCG CCATTAAAGG ACTAAAACCT AATGCAATTT ACCTTTTCCT TGTGAGAGCA 
GCAAATGCAT ATGGAATTAG TGATCCAAGC CAGATATCAG ATCCAGTGAA AACGCAAGAT 
GTTCCCCCAA CGAGTCAGGG AGTAGATCAC AAGCAGGTCC AGAGAGAATT GGGAAATGTT 
GTACTGCACC TGCACAACCC CACCATCCTT TCTTCCTCTT CAATCGAAGT ACACTGGACA 
GTGGACCAAC AATCTCAGTA TATTCAAGGA TATAAAATTC TTTATCGGCC ATCAGGAGCC 
AACCATGGTG AATCGGAGTG GTTAGTTTTT GAAGTGAGGA CCCCAACCAA AAATAGTGTG 
GTTATCCCTG ATCTCAAAAA GGGAGTCAAC TATGAAATTA AGGCTCGTCC TTTTTTTAAT 
GAATTTCAAG GAGCAGATAG TGAAATTAAG TTCGCAAAGA CCTTAGAAGA AGCACCCAGT 
GCCCCACCCC AAAGTGTAAC TGTGTCAAAG AATGATGGAA ATGGAACTGC AATTCTTGTT 
AGTTGGCAGG CGCCTCCAGA AGACACTCAA AACGGAATGG TCCAAGAGTA TAAGGTTTGG 
TGTCTGGGCA ATGAAACTAG ATATCATATA AACAAGACAG TAGATGGTTC CACTTTTTCT 
GTGGTCATCC CTTCTCTGGT TCCTGGGATC CGGTACAGCA TAGAGGTGGC AGCCAGCACT 
GGGGCCGGGG CCGGGGTGAA GAGCGAGCCA CAGTTCATCC AGTTAGATTC TCATGGAAAC 
CCTGTGTCGC CTGAAGACCA GGTCAGCCTT GCTCAGCAGA TCTCCGATGT GGTGAAGCAG 
CCAGCGTTCA TTGCCGGAAT CGGGGCAGCC TGCTGGATCA TCCTCATGGT GTTTAGCATC 
TGGCTTTATC GGCACCGCAA GAAGAGAAAC GGGCTGACTA GTACTTACGC GGGTATCAGA 
AAAGTAACTT ATCAGAGAGG CGGTGAAGCT GTAAGCAGTG GAGGGAGGCC AGGACTTCTG 
AACATCGGTG AACCTGCCAC ACAGCCATGG CTGGCCGACA CATGGCCTAA TACTGGCAAC 
AGTCACAATG ACTGCTCCAT CACTTGCTGC ACTGCAGCCA ATGGTAACAG TGATAGCAAC 
CTGACCACCT ACAGTCGCCC AGGGCAGCCC ACTCCTTACG CCACCACCCA GCTCATCCAG 
TCCAACCTCA GCAACAACAT GAACAATGGC AGTGGGGACC CCAGCGAGAA GCACTGGAAG 
CCACCTGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG AGCAAATGAC ACAATTCCTC CAACTATCCC ATACAACCAG 
TCATACGACC AGAATACAGG AGGATCTTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TTCCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CGCCTCACAG CAATAGTGAA 
GAGTACAGCA TTTCTGTAGA TGAAAGTTAT GACCAAGAAA TGCCGTGTCC TGTGCCACCA 
GCCAGGATGT ATTTGCAACA GGACGAACTG GAAGAGGAGG AAGATGAACG AGGCCCCACC 
CCCCCTGTAC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTACAGCCA TCAGTCCACT 
GCGACACTGA CCCCGTCTCC ACAAGAAGAA CTCCAGCCCA TGTTACAGGA TTGCCCAGAG 
GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCCCCTCCG 
CCACCACGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA 
GATATGGATA CGGATGCTCC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG 
ATGCAGACCA GACGGCTTTT GTTACGTGGG CTTGAGCAGA CCCCTGCCTC CAGTGTAGGG 
GACCTCGAGA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGTGGACG CTCCAGTGTT AGTTCTTCCG ATGGCTCCTT CTTCACCGAT 
GCCGATTTTG CCCAGGCAGT GGCGGCAGCA GCAGAGTATG CTGGCCTCAA AGTGGCACGT 
CGCCAAATGC AGGATGCCGC TGGCCGCCGA CATTTTCATG CATCCCAGTG CCCAAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACATG AGTGCTGCGG TAATACAGAA GGCCAGACCA 
GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCTC CAGTGCCACC ACCTGCTATA AAGTCACCCA CTGTTCAATC CAAGGCACAG 
CTGGAGGTAC GACCTGTAAT GGCACCCAAA CTACCTTCTA TAGAAGCAAG AACAGACAGG 
TCATCAGATA GAAAAGTAGG CAGCTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCAGATATGA GAACAAATCC AGGTGATACC AGAGAAGCAC AGGAGCAGCA AAATGATGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGC GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTTCTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO1 ( XM_019839543.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019695102.2 Felis catus roundabout guidance receptor 1 (ROBO1), mRNA.

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CloneID	OFb60452
Clone ID Related Accession (Same CDS sequence)	XM_019839543.2
Accession Version	XM_019839543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	LOW QUALITY PROTEIN: roundabout homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018731.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019839543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 3 indels ##RefSeq-Attributes-END##

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGGCGAAC TGTAACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA 
GCTATTTTTT GGAGACGAGA GGGGAGTCAG AATTTGCTTT TCTCATATCA GCCACCACAG 
TCAACCAGCC GTTTTTCAGT CTCCCAGACT GGTGATCTCA CTATCACTAA CGTCCAGCGA 
TCTGATGTTG GTTATTATAT CTGCCAGACT TTAAATGTTG CTGGAAGTAT CATCACAAAA 
GCATATTTGG AAGTAACTGA TGTGATTGCA GACCGGCCTC CCCCGGTCAT TCGACAAGGT 
CCTGTGAATC AGACTGTGGC CGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC 
AGTCCAGCAC CCACGATTCT TTGGAGAAAG GATGGAGTGC TTGTTTCAAC CCAAGATTCT 
CGAATCAAAC AGCTGGAGAC AGGAGTGCTG CAGATCCGAT ATGCTAAGCT GGGTGACACA 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAGG CAACATGGAG TGCTTACATT 
GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACTGACCC AAATTTAATC 
CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCA GAAATACGGT AACCTTGTCA 
TGGCAACCAA ATTTGAATTC AGGTGCAACT CCAACATCTT ACATTATAGA AGCCTTCAGC 
CACGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACGGA AACATTCGCC 
ATTAAAGGAC TAAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGAGCAGC AAATGCATAT 
GGAATTAGTG ATCCAAGCCA GATATCAGAT CCAGTGAAAA CGCAAGATGT TCCCCCAACG 
AGTCAGGGAG TAGATCACAA GCAGGTCCAG AGAGAATTGG GAAATGTTGT ACTGCACCTG 
CACAACCCCA CCATCCTTTC TTCCTCTTCA ATCGAAGTAC ACTGGACAGT GGACCAACAA 
TCTCAGTATA TTCAAGGATA TAAAATTCTT TATCGGCCAT CAGGAGCCAA CCATGGTGAA 
TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT TATCCCTGAT 
CTCAAAAAGG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATTAAGTT CGCAAAGACC TTAGAAGAAG CACCCAGTGC CCCACCCCAA 
AGTGTAACTG TGTCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGGCG 
CCTCCAGAAG ACACTCAAAA CGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTAGAT ATCATATAAA CAAGACAGTA GATGGTTCCA CTTTTTCTGT GGTCATCCCT 
TCTCTGGTTC CTGGGATCCG GTACAGCATA GAGGTGGCAG CCAGCACTGG GGCCGGGGCC 
GGGGTGAAGA GCGAGCCACA GTTCATCCAG TTAGATTCTC ATGGAAACCC TGTGTCGCCT 
GAAGACCAGG TCAGCCTTGC TCAGCAGATC TCCGATGTGG TGAAGCAGCC AGCGTTCATT 
GCCGGAATCG GGGCAGCCTG CTGGATCATC CTCATGGTGT TTAGCATCTG GCTTTATCGG 
CACCGCAAGA AGAGAAACGG GCTGACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGCGGTG AAGCTGTAAG CAGTGGAGGG 
AGGCCAGGAC TTCTGAACAT CGGTGAACCT GCCACACAGC CATGGCTGGC CGACACATGG 
CCTAATACTG GCAACAGTCA CAATGACTGC TCCATCACTT GCTGCACTGC AGCCAATGGT 
AACAGTGATA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA ANACAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAC 
GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCTTACG CCACCACCCA GCTCATCCAG 
TCCAACCTCA GCAACAACAT GAACAATGGC AGTGGGGACC CCAGCGAGAA GCACTGGAAG 
CCACCTGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG AGCAAATGAC ACAATTCCTC CAACTATCCC ATACAACCAG 
TCATACGACC AGAATACAGG AGGATCTTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TTCCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CGCCTCACAG CAATAGTGAA 
GAGTACAGCA TTTCTGTAGA TGAAAGTTAT GACCAAGAAA TGCCGTGTCC TGTGCCACCA 
GCCAGGATGT ATTTGCAACA GGACGAACTG GAAGAGGAGG AAGATGAACG AGGCCCCACC 
CCCCCTGTAC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTACAGCCA ACAGTCCACT 
GCGACACTGA CCCCGTCTCC ACAAGAAGAA CTCCAGCCCA TGTTACAGGA TTGCCCAGAG 
GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCCCCTCCG 
CCACCACGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA 
GATATGGATA CGGATGCTCC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG 
ATGCAGACCA GACGGCTTTT GTTACGTGGG CTTGAGCAGA CCCCTGCCTC CAGTGTAGGG 
GACCTCGAGA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGTGGACG CTCCAGTGTT AGTTCTTCCG ATGGCTCCTT CTTCACCGAT 
GCCGATTTTG CCCAGGCAGT GGCGGCAGCA GCAGAGTATG CTGGCCTCAA AGTGGCACGT 
CGCCAAATGC AGGATGCCGC TGGCCGCCGA CATTTTCATG CATCCCAGTG CCCAAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACATG AGTGCTGCGG TAATACAGAA GGCCAGACCA 
GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCTC CAGTGNCACC ACCTGCTATA AAGTCACCCA CTGTTCAATC CAAGGCACAG 
CTGGAGGTAC GACCTGTAAT GGCACCCAAA CTACCTTCTA TAGAAGCAAG AACAGACAGG 
TCATCAGATA GAAAAGTAGG CAGCTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCAGATATGA GAACAAATCC AGGTGATACC AGAGAAGCAC AGGAGCAGCA AAATGATGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGC GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTTCTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019695102.2
CDS	147..5102
Translation	MKWKHVPFLVIISLLSLTEDHLFLAQLIPDPEDLERGNDNGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVMAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSTSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLVLSCVATGSPAPTILWRKDGVLVSTQDSRIKQLETGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGVDHKQVQRELGNVVLHLHNPTILSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESEWLVFEVRTPTKNSVVIPDLKKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQSVTVSKNDGNGTAILVSWQAPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPSLVPGIRYSIEVAASTGAGAGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNIGEPATQPWLADTWPNTGNSHNDCSITCCTAANGNSDSNLTTYSRPADCIANYNNQLDNKQnullNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDPSEKHWKPPGQQKQEVAPIQYNIMEQNKLNKDYRANDTIPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYSISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSQQSTATLTPSPQEELQPMLQDCPEEIGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPAKKQKHQPGHLRREAYTDDLPPPPVnullPPAIKSPTVQSKAQLEVRPVMAPKLPSIEARTDRSSDRKVGSYKGREVLDGRQVADMRTNPGDTREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

Target ORF information:

RefSeq Version	XM_019839543.2
Organism	Felis catus(domestic cat)
Definition	Felis catus roundabout guidance receptor 1 (ROBO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019839543.2

ORF Insert Sequence:

ATGAAATGGA AACATGTTCC TTTTTTGGTC ATAATATCAC TCCTCAGCTT GACAGAAGAT 
CACCTGTTCC TGGCACAGCT TATTCCAGAC CCTGAAGACC TAGAGAGGGG GAACGACAAC 
GGGACGCCCA TCCCCACCTC TGATAATGAT GACAATTCGC TGGGCTACAC AGGCTCCCGT 
CTTCGTCAGG AAGATTTTCC ACCTCGCATT GTTGAACACC CTTCGGACCT AATTGTGTCA 
AAAGGAGAAC CTGCAACTTT GAACTGTAAA GCTGAAGGCC GCCCCACACC CACTATCGAA 
TGGTACAAAG GGGGGGAGAG AGTGGAGACG GACAAAGATG ACCCTCGATC ACACCGAATG 
TTGCTGCCAA GTGGATCTTT ATTTTTCTTA CGTATAGTAC ATGGACGAAA AAGCAGGCCT 
GATGAAGGGG TCTACGTCTG TGTAGCAAGG AATTACCTCG GAGAGGCCGT GAGCCATAAT 
GCATCGCTGG AGGTGGCTAT ACTCCGGGAT GACTTCAGAC AAAATCCTTC CGATGTCATG 
GTGGCAGTGG GGGAGCCTGC AGTGATGGAA TGCCAGCCTC CACGAGGCCA TCCTGAACCC 
ACCATTTCAT GGAAGAAAGA TGGCTCTCCG CTGGATGACA AAGATGAACG AATAACTATT 
CGAGGAGGAA AGCTCATGAT CACTTACACC CGCAAAAGTG ATGCTGGCAA ATACGTTTGT 
GTTGGTACCA ACATGGTTGG GGAACGGGAG AGTGAAGTGG CTGAGCTAAC GGTTTTGGAG 
AGACCATCGT TTGTAAAGAG ACCTAGTAAC TTGGCGGTAA CTGTGGACGA CAGTGCGGAA 
TTTAAATGTG AGGCCCGAGG TGACCCTGTG CCTACGGTAC GATGGAGGAA AGATGATGGA 
GAACTGCCCA AATCCAGATA TGAAATCAGA GATGATCATA CCTTGAAAAT TCGGAAGGTG 
ATGGCTGGTG ACATGGGGTC ATATACTTGT GTTGCGGAAA ATATGGTTGG AAAAGCGGAA 
GCATCAGCTA CTCTGACTGT CCAAGAACCT CCACATTTTG TTGTGAAACC CCGTGACCAG 
GTTGTTGCTT TGGGGCGAAC TGTAACTTTT CAGTGTGAAG CAACTGGAAA TCCTCAACCA 
GCTATTTTTT GGAGACGAGA GGGGAGTCAG AATTTGCTTT TCTCATATCA GCCACCACAG 
TCAACCAGCC GTTTTTCAGT CTCCCAGACT GGTGATCTCA CTATCACTAA CGTCCAGCGA 
TCTGATGTTG GTTATTATAT CTGCCAGACT TTAAATGTTG CTGGAAGTAT CATCACAAAA 
GCATATTTGG AAGTAACTGA TGTGATTGCA GACCGGCCTC CCCCGGTCAT TCGACAAGGT 
CCTGTGAATC AGACTGTGGC CGTGGATGGT ACTTTAGTGC TCAGCTGTGT GGCCACAGGC 
AGTCCAGCAC CCACGATTCT TTGGAGAAAG GATGGAGTGC TTGTTTCAAC CCAAGATTCT 
CGAATCAAAC AGCTGGAGAC AGGAGTGCTG CAGATCCGAT ATGCTAAGCT GGGTGACACA 
GGTCGGTACA CCTGCATTGC ATCAACCCCC AGTGGTGAGG CAACATGGAG TGCTTACATT 
GAAGTCCAAG AATTTGGAGT TCCAGTTCAG CCTCCAAGAC CCACTGACCC AAATTTAATC 
CCTAGTGCCC CCTCAAAACC TGAAGTTACA GATGTCAGCA GAAATACGGT AACCTTGTCA 
TGGCAACCAA ATTTGAATTC AGGTGCAACT CCAACATCTT ACATTATAGA AGCCTTCAGC 
CACGCATCTG GTAGCAGCTG GCAGACCGTA GCAGAGAATG TGAAAACGGA AACATTCGCC 
ATTAAAGGAC TAAAACCTAA TGCAATTTAC CTTTTCCTTG TGAGAGCAGC AAATGCATAT 
GGAATTAGTG ATCCAAGCCA GATATCAGAT CCAGTGAAAA CGCAAGATGT TCCCCCAACG 
AGTCAGGGAG TAGATCACAA GCAGGTCCAG AGAGAATTGG GAAATGTTGT ACTGCACCTG 
CACAACCCCA CCATCCTTTC TTCCTCTTCA ATCGAAGTAC ACTGGACAGT GGACCAACAA 
TCTCAGTATA TTCAAGGATA TAAAATTCTT TATCGGCCAT CAGGAGCCAA CCATGGTGAA 
TCGGAGTGGT TAGTTTTTGA AGTGAGGACC CCAACCAAAA ATAGTGTGGT TATCCCTGAT 
CTCAAAAAGG GAGTCAACTA TGAAATTAAG GCTCGTCCTT TTTTTAATGA ATTTCAAGGA 
GCAGATAGTG AAATTAAGTT CGCAAAGACC TTAGAAGAAG CACCCAGTGC CCCACCCCAA 
AGTGTAACTG TGTCAAAGAA TGATGGAAAT GGAACTGCAA TTCTTGTTAG TTGGCAGGCG 
CCTCCAGAAG ACACTCAAAA CGGAATGGTC CAAGAGTATA AGGTTTGGTG TCTGGGCAAT 
GAAACTAGAT ATCATATAAA CAAGACAGTA GATGGTTCCA CTTTTTCTGT GGTCATCCCT 
TCTCTGGTTC CTGGGATCCG GTACAGCATA GAGGTGGCAG CCAGCACTGG GGCCGGGGCC 
GGGGTGAAGA GCGAGCCACA GTTCATCCAG TTAGATTCTC ATGGAAACCC TGTGTCGCCT 
GAAGACCAGG TCAGCCTTGC TCAGCAGATC TCCGATGTGG TGAAGCAGCC AGCGTTCATT 
GCCGGAATCG GGGCAGCCTG CTGGATCATC CTCATGGTGT TTAGCATCTG GCTTTATCGG 
CACCGCAAGA AGAGAAACGG GCTGACTAGT ACTTACGCGG GTATCAGAAA AGTCCCGTCT 
TTTACCTTCA CACCAACAGT AACTTATCAG AGAGGCGGTG AAGCTGTAAG CAGTGGAGGG 
AGGCCAGGAC TTCTGAACAT CGGTGAACCT GCCACACAGC CATGGCTGGC CGACACATGG 
CCTAATACTG GCAACAGTCA CAATGACTGC TCCATCACTT GCTGCACTGC AGCCAATGGT 
AACAGTGATA GCAACCTGAC CACCTACAGT CGCCCAGCTG ATTGTATAGC AAATTATAAC 
AACCAACTGG ATAACAAACA ANACAATCTG ATGCTCCCTG AGTCAACTGT TTATGGTGAT 
GTGGACCTTA GTAACAAAAT CAATGAGATG AAAACCTTCA ATAGCCCAAA TCTGAAGGAC 
GGGCGTTTTG TCAATCCATC AGGGCAGCCC ACTCCTTACG CCACCACCCA GCTCATCCAG 
TCCAACCTCA GCAACAACAT GAACAATGGC AGTGGGGACC CCAGCGAGAA GCACTGGAAG 
CCACCTGGAC AGCAGAAACA GGAAGTGGCA CCAATTCAGT ATAACATCAT GGAGCAAAAC 
AAACTGAACA AAGATTATCG AGCAAATGAC ACAATTCCTC CAACTATCCC ATACAACCAG 
TCATACGACC AGAATACAGG AGGATCTTAC AACAGTTCAG ACCGGGGCAG TAGTACATCT 
GGGAGTCAAG GGCACAAGAA AGGGGCACGG ACACCCAAAG TTCCAAAACA GGGTGGCATG 
AACTGGGCAG ACCTGCTTCC TCCTCCCCCA GCACATCCTC CGCCTCACAG CAATAGTGAA 
GAGTACAGCA TTTCTGTAGA TGAAAGTTAT GACCAAGAAA TGCCGTGTCC TGTGCCACCA 
GCCAGGATGT ATTTGCAACA GGACGAACTG GAAGAGGAGG AAGATGAACG AGGCCCCACC 
CCCCCTGTAC GGGGAGCAGC TTCTTCTCCA GCTGCTGTGT CTTACAGCCA ACAGTCCACT 
GCGACACTGA CCCCGTCTCC ACAAGAAGAA CTCCAGCCCA TGTTACAGGA TTGCCCAGAG 
GAGATTGGCC ACATGCAGCA CCAACCCGAT AGGAGACGGC AGCCTGTGAG TCCCCCTCCG 
CCACCACGAC CGATTTCCCC ACCACATACC TATGGCTACA TTTCAGGACC TCTTGTCTCA 
GATATGGATA CGGATGCTCC AGAAGAGGAA GAAGATGAAG CAGACATGGA AGTGGCCAAG 
ATGCAGACCA GACGGCTTTT GTTACGTGGG CTTGAGCAGA CCCCTGCCTC CAGTGTAGGG 
GACCTCGAGA GCTCTGTCAC TGGGTCCATG ATCAATGGCT GGGGCTCAGC CTCAGAGGAG 
GACAACATTT CCAGTGGACG CTCCAGTGTT AGTTCTTCCG ATGGCTCCTT CTTCACCGAT 
GCCGATTTTG CCCAGGCAGT GGCGGCAGCA GCAGAGTATG CTGGCCTCAA AGTGGCACGT 
CGCCAAATGC AGGATGCCGC TGGCCGCCGA CATTTTCATG CATCCCAGTG CCCAAGACCC 
ACAAGTCCTG TTTCTACAGA CAGCAACATG AGTGCTGCGG TAATACAGAA GGCCAGACCA 
GCCAAGAAGC AAAAACACCA GCCAGGACAT CTGCGCAGAG AAGCCTACAC AGATGATCTT 
CCACCTCCTC CAGTGNCACC ACCTGCTATA AAGTCACCCA CTGTTCAATC CAAGGCACAG 
CTGGAGGTAC GACCTGTAAT GGCACCCAAA CTACCTTCTA TAGAAGCAAG AACAGACAGG 
TCATCAGATA GAAAAGTAGG CAGCTACAAG GGGAGAGAAG TATTGGATGG AAGACAAGTT 
GCAGATATGA GAACAAATCC AGGTGATACC AGAGAAGCAC AGGAGCAGCA AAATGATGGG 
AAAGGACGAG GAAACAAGGC AGCAAAACGC GACCTTCCAC CAGCAAAGAC TCATCTCATC 
CAAGAGGATA TTCTACCTTA CTGTAGACCT ACTTTTCCGA CATCAAATAA TCCCAGAGAT 
CCTAGTTCTT CAAGCTCAAT GTCATCAAGA GGATCAGGAA GCAGACAAAG AGAACAAGCA 
AATGTAGGTC GAAGAAATAT TGCAGAAATG CAGGTTCTTG GAGGATATGA AAGAGGAGAA 
GATAATAATG AAGAATTAGA GGAAACTGAA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ROBO1
Entrez Gene ID	101093131
Full Name	roundabout guidance receptor 1
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ROBO1 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

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