F8 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F8
Entrez Gene ID	101092751
Full Name	coagulation factor VIII
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004001054.4	XP_004001103.1	coagulation factor VIII isoform X1
XM_019824515.1	XP_019680074.1	coagulation factor VIII isoform X2
XM_019824518.1	XP_019680077.1	coagulation factor VIII isoform X3
XM_019824516.1	XP_019680075.1	coagulation factor VIII isoform X2
XM_019824515.2	XP_019680074.1	coagulation factor VIII isoform X2
XM_019824517.1	XP_019680076.1	coagulation factor VIII isoform X3

Pathways from BioSystems
Gene Ontology

The following F8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb24325	XM_004001054.4 Latest version!	Felis catus coagulation factor VIII (F8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OFb58139	XM_019824516.1 Latest version!	Felis catus coagulation factor VIII (F8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb58139	XM_019824515.2 Latest version!	Felis catus coagulation factor VIII (F8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb58140	XM_019824517.1 Latest version!	Felis catus coagulation factor VIII (F8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb58141	XM_019824518.1 Latest version!	Felis catus coagulation factor VIII (F8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OFb58139	XM_019824515.1	Felis catus coagulation factor VIII (F8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F8 ( XM_004001054.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_004001103.1 Felis catus coagulation factor VIII (F8), transcript variant X1, mRNA.

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CloneID	OFb24325
Clone ID Related Accession (Same CDS sequence)	XM_004001054.4
Accession Version	XM_004001054.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	coagulation factor VIII isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_004001054.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAATAG AACTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCCATTCAG CTTCAGTGCC 
ACAAGAAAAT ACTACCTGGG CGCAGTGGAA CTGTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCACGTGGA CACAAGGTTA TCCCCTGGGG TGCCAAGATC TTTGCCATTC 
AACACATCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TTACAGATCG CCTTTTCAAC 
ATTGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CTACCATCCG GGCCGAAGTT 
TTTGACACAG TGGTCATTAC ACTCAAGAAC ATGGCTTCTC ACCCTGTCAG TCTTCATGCT 
GTTGGTGTAT CCTATTGGAA AGCTTCTGAA GGTGCTGAAT ATGAGGACCA GACCAGCCAG 
AAGGAGAAGG AAGATGATAA AGTCTTTCCT GGTGAGAGTC ATACCTACGT CTGGCAGGTC 
CTGAAAGAGA ATGGACCGAT GGCCTCGGAT CCTCCATGTC TCACCTACTC ATATTTTTCT 
CATGTGGACC TGGTGAAAGA CCTGAATTCA GGCCTCATTG GAGCCCTGCT GGTTTGCAAA 
GAAGGAAGTC TGGCTGAAGA AAGGACACAG ACCTTGCATG AATTTGTACT CCTGTTTGCT 
GTATTTGATG AAGGAAAAAG CTGGCACTCA TCTGCTCAGG CCCAGCATGA AATGCACACG 
GTCAACGGCT ATGTCAACAG GTCTCTGCCA GGTCTGACTG GATGCCACAA GAAATCAGTC 
TATTGGCATG TGATTGGAAT GGGCACCACC CCCGAAGTGC ACTCAATTTT TCTTGAAGGT 
CACACATTTC TCATCAGAAA CCACCGCCAG GCCTCCTTGG AGATCTCACC AATAACTTTC 
CTCACTGCTC AGACATTCCT GATGGACCTT GGCCAGTTTC TACTGTTTTG TCACATCTCT 
TCCCACCAGC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC AGAGGAACCC 
CAGCTACGGA TGAAAACTAA TGAAAAAGAG GAAGATTATG ATGATGATCT TTATGATTCT 
GACATGGACA TGGTTAGGTT TGATGGCGAC AGCCCTTCTC CCTTTATTCA AATCCGCTCA 
GTTGCCAAGA AGCATCCTAA AACTTGGATC CACTATATTG CCGCCGAAGA GGAGGACTGG 
GACTATGCTC CCTCGGCCCC CACCCCCATT GACAGAAGTT ATAAACATCT ATATTTGAAC 
AGTGGTCCTC AGCGGATTGG TAGGAAGTAC AAAAAAGTTC GATTTATGGC ATACACAGAT 
GAAACATTTA AGACTCGTGA AGCTATTCAG TATGAATCAG GAATCCTAGG ACCTTTACTT 
TATGGAGAAG TTGGAGACAC ACTGTTGATT ATATTTAAAA ATCAAGCAAG CCGACCATAT 
AACATCTACC CTCATGGAAT CACTGACGTC AGTCCTTTGT ACTCAGGGAG ATTGCCTAAA 
GGTATGAAAC ATTTGAAAGA TATGCCAATT ATGCCAGGAG AGATATTCAA GTATAAATGG 
ACAGTGACTG TAGAAGATGG GCCGACTAAA TCAGATCCTC GGTGCCTGAC TCGATATTAC 
TCGAGCTTCA TTAATCCAGA GAGAGATCTA GCTTCAGGAC TCATTGGCCC TCTCCTCATA 
TGCTACAAAG GATCTGTAGA TCAAAGAGGA AACCAGATGA TGTCAGACAA GAGAAACGTC 
ATCCTGTTTT CTGTATTTGA TGAGAACCAA AGCTGGTACC TTGCAGAGAA TATGAAGCGC 
TTCCTCCCGA ATGCAGATGC AGTGCCGCCC CACGACCCGG AGCTCCAGGT CTCTAACATC 
ATGCACAGCA TCAACGGCTA TGTTTTTGAC AACTTGCAGC TGTCAGTTTG TTTGCATGAG 
GTGGCATACT GGTACATTCT AAGTGTTGGA GCACAAACTG ACTTCCTGTC TGTCTTCTTC 
TCTGGATACA CCTTCAAACA CAAAATGGTC TATGAAGACA CACTTACCCT ATTCCCATTC 
TCAGGAGAAA CTGTCTTCAT GTCAATGGAA AATCCAGGTC TATGGGTGCT GGGGTGCCAC 
AACTCAGACT TTCGGAACAG AGGCATGACA GCTTTACTGA AGGTTTCTAG TTGTAATGGG 
AACATTGATG ATTATGATGA GGATAGATAT GAAGATATTC CAGCCTCCCT CCTGAATGAA 
AACAATGTCA TTGAACCCAG AAGCTTCTCC CAGAATTCAA GACATCATAG CCCTAGGCAA 
AAGCAATTCA AAGCCACCAC AACTCCAGAA AATGACATAG AGAAGATTGA CCCTCAGCCT 
GGAGAAAGAA CGCAGCTGCT TAACGTACAA AGTGTCTCCT CTAGTGATTT GCTGATGCTT 
CTGGGACAGA ATCCTACTCC ACGTGGATTA CTCTTATCTG ATTTCCAGGA AGCCACACAT 
GAAGCAGATG ATCATTTACT TGGAGCTATA GAAAGACACA AGGGCCCATC TGCAGTGGCA 
GATCTCAGAC CAGAGCTCCA TCACAGTGGG GACAGAGTAT TTACTCCTGA GCCAAAACTA 
AAATTAAGAT TAAATGAGAA TTTGGGGACA ACTGTAACAG CAGAGTTGAA GAACCTTGAT 
TTAAAAGTTT CTAGTTCATC AGACAACCTA ACAGCTTCAC CAACAATTCC ATCAGACAAG 
TTGGCAGCAG GTACTGAAAA GACAGGTTCC TTAGGACCCC CAAATATGTC AATTCACTTA 
AATAGTCATT TAGGTACTAT TGTATTTGGC AAAAATTCAT CTCACTCTAT TGACTCTGGT 
GTACCTTTGG GCTCGAGTGA AGGAGACAAT GATTCCAAGT TGTTAGAAGC AGCTTTAATG 
AATAGTCAAG AAAGTTCACT GGGAGAAAAT GTATTATCAG TGGAGAGAAA TGGGTTATTT 
AAAGAAGACA GAGTTCATGG ACCTGCTTCA TTAATTAAAG ATAATGCTTT ATTCAAAGTT 
AATATCTCTT TGATAAAGAC AAACAACGCA ACAATTAACT CAACAACTAA TAGCAAAAAT 
CATGTTGATG TCCCAACATT ATTAACTGAG AACAGTACAT CAGTCTGGAA AGATACTATA 
TTAGAAAGTA ATGCTGAGTT TCAAGAAATG ACTTCTTTGC TTCATAATGA AACATTTATG 
GACAGAAATA TTACAGCTTT GGAGCTAAAG CATGTGTCAA ATAAAACTAC TTCATCAAAA 
AATGTGGAAG TGGTCTACCA AAACAAAGAG GGCTCGGTGC CACTAGGTGC AGAAAATCCA 
GGTCTCTCAT TCTTCAAGAC ACTGTTCTCA CCAGATTCAG CAAAGTGGAT AAAATGGACC 
CATGGCGAGA ACTCCCTAAG CTCTGGGCAA AGGCCCATTC CAACACAATT AACATCTTTA 
GGATCAGAAA AATCTGTGAA AGATCAGAAG TTTTTGTCAG AAAAGAAGGT GGTAGTAGGA 
GAAGATGAAT TTACAAAGGA CACAGAACTC AAAGAGATGA GTTTTCCAAA CAGCAAGAGC 
ATGTTTTTTA CTAACTTGCC CAATGTCCAA GATAATGGTC CTTACAATCA AGAAGAAAAA 
TCTCAGGAAG AGATAGAAAG AAAGGAGAAA TTAACCCAAG AAAATGTGGT TTTGCCTCAG 
GAATATACAG TAGCTGACAC TAATAATTCC CTGAAGAACC TTTTCTTACT AAGCACTAAG 
CAAAATGTGG AAGGTTTAGA TGAGGGGCCA TATACTCCAA TACTTCAAGA CACCAGGTCA 
TTAAATGATT CAGCACATAG AGCAGGGATT CACATAGTCC ATTTCTCAAA AATAAGGGAG 
GAAGAAAACT TGGAAGGTTT GGGAAATCAA ACAAAGGAAA TGGCAGAGAG GTTTTCAAGC 
ACTCCAAGGA TGTCTCCTAA TCCAAGTCAG CATAATATTC TCACTCAGCG TGGGAAGCGG 
GCCTTGAAAC AATCCAGACT CTCACTAGAA GAAACTAAGC TTGCAAGGGG GGTAATTTTG 
AATGACACCT CAACCCAGTC ATCCAAAAAC ATGAACTTTT TGATGCATGG AACCCTCACA 
CAGATAGAGG ACAATGAGAA AGAAAAAGGG GCTGCCACTC AGTCCCTCCA ATCAGATTGT 
TCTATGGGGA ATCAGGGCAC CATTCAAATG AATGACTCTG CATTACCCAT TGCAAAGGAA 
TCAGCATTTC CATCAATCAG ACATACAGAT CTGACCAAGA TCCCATCCCA GGACAACTCT 
TCTCATCTTC CAGTATCAGC CTGCACTTAT ACCTTCAGGG AGAGTTCTGG GGTCCAAAAA 
AGCACTCATT TCTCACAAGG AGCCAAGAGA AATAAACTTT CTGTAGCCTT CCTAACCTCA 
GAAATGATTG TAGGTCAAGG AAAGTTCAGC TCCCTAGGGA AAAGTGCCAT GAACCAACGC 
ATGTCCCAGA AACGTGAAAA CACTCTTTTG CAACAAGGCT TGTCTGAAGC ATCTGGCAAA 
GTTGAATTAC TCCCTAAAGC TCATGTTCAT CAAGAAGACT CTTTCCCTAC AAAAACTAGC 
AATGATTCTT CTGGCCACCT GGATCTTATG AAGAAGATCT TCCTTCGGAA AACACAGGGA 
CCTAAGAAAA TAAATGGACC CGGAAAAGTG CCCTTCCTGA AATGGACAAC AGAGAGCTCT 
GAAAAGATTC CCTCTGAGCT GCTGGGTCCC CTTGCTTGGG ATAACAACAA TGCCACCCAG 
ATACCGAGAG AAGAGTGGAA ATCCCAAAAG TCACAGAAAA ACATAGCTTT TAAGAGCAAG 
GACACCATTT TGCCCTTGGG CCCTTGTGAA AACAGTCCTT CAGTAGCAGC AATAAATGAA 
GGGCAAGGTA AACTCCAAAG AGAAGCCACC AGGTCAAAGC AAGGAGAGAC TGGAAGATTG 
TGCTCTCAGA ACCCACCGGT CTCAAAACGC CATCAAAGAG AAATAACCCT TACTACTCTT 
CAGTCAGAGG AAGACAAAAT TGAGTATGAT GATACGTTTT CAATTGAAAT GAAGAGAGAA 
GATTTTGACA TCTATGGTGA AGATGAAAAT CAGGGCCTCC GCAGCTTTCA AAAGAGAACA 
CGACACTATT TCATTGCTGC AGTGGAGCGT CTCTGGGATT ATGGGATGAG TAGATCTCCC 
CATGCACTAA GAAACAGGGC TCCAGGTGGG GATGTCCCTC AGTTCAAGAA GGTGGTTTTC 
CAGGAATTTA CTGATGGATC CTTCACTCAG CCCTTATACC GTGGAGAGCT GAATGAACAT 
TTGGGACTCT TGGGGCCATA TATAAGAGCA GAAGTTGAAG ACAATATCAT GGTAACTTTC 
AAAAACCAGG CTTCTCGTCC CTACTCCTTC TATTCTAGCC TGATTTCTTA TGAGGAAGAT 
CAGAGGCAAG GAGCAGAACC TAGAAAAAAG TTTGTCAACC CTAATGAAAC CAGAGTTTAC 
TTCTGGAAAG TACAACATCA GATGGCACCC ACTGAAGATG AGTTTGACTG CAAGGCTTGG 
GCTTATTTTT CTGATGTTGA TTTGGAAAAA GACATGCACT CGGGCTTGAT TGGACCCCTT 
CTGATCTGCC GCAGTAACAC ACTGAGCCCC GCTCACGGGA GACAAGTGAC AGTGCAGGAG 
TTTGCTCTGT TTTTCACCAT TTTTGATGAG ACTAAGAGCT GGTACTTCAC GGAAAACATG 
GAAAGGAGCT GTAGAGCCCC CTGCAGTATC CAGAAGGAGG ATCCCACTTT TAAAGAAAAG 
TACCGCTTCC ACGCAATCAA CGGCTATGTG ATGGACACAC TACCCGGCTT GGTAGTGGCT 
CAGGATCAGA AGATCCGATG GTATCTGCTC AGCATGGGGA GCAATGAAAA CATTCATTCT 
ATTCATTTCA GTGGGCAGGT GTTCACTGTA CGGAAAAAAG AGGAATATAA GATGGCAGTC 
TACAACCTGT ATCCAGGTGT TTTTGAGACA GTGGAAATGC TACCATCCAA AGTTGGAATT 
TGGCGGATAG AATGCCTAAT TGGCGAACAC CTGCAAGCTG GGATGAGCAC TCTCTTTCTG 
GTATACAGCC CGAAGTGTCA GATTCCACTG GGAATGGCTT CTGGACGCAT TAGAGATTTC 
CAGATCACAG CTTCAGGACA GTATGGACAG TGGGCCCCAA AGCTGGCCAG ACTTCACTAT 
TCTGGATCAA TCAATGCCTG GAGCACCAAG GACCCCATTT CCTGGATCAA GGTGGATCTG 
TTGGCACCAA TGATCATTCA CAGCATCATG ACCCAGGGCG CCCGTCAGAA GTTGTCCAGC 
CTGTACATCT CTCAGTTTAT CATCATGTAT AGTCTTGATG GAAAGAAGTG GCAGAGTTAT 
CGAGGGAATT CCACTGGGAC CTTAATGGTG TTCTTTGGCA ATGTGGATTC ATCTGGGATA 
AAACACAATA TTTTTAACCC TCCGATTATT GCTCGGTACA TCCGTTTGCA CCCAACGCAT 
TACAGCATCC GCAGCACTCT CCGCATGGAG TTGATGGGCT GTGATTTAAA CAGCTGCAGC 
ATGCCACTGG GAATGGAGAG TAAAGCAATA TCAGACGCAC AGGTCACCGC CTCGTCCTAC 
TTAAACAGCA TGTTTGGCAC TTGGTCCCCT TCACAAGCCC GGCTGCACCT CCAGGGGAGG 
ACGAATGCCT GGAGGCCTCA GGCAAATAAT CCAAAAGAGT GGCTGCAAGT GGACTTCCAG 
AAGACAATGA AAGTCACAGG AATAGCCACC CAGGGGGTGA AATCACTCCT TACCAGCATG 
TATGTGAAGG AGTTCCTCGT TTCCAGTAGT CAAGATGGCC ATAACTGGGC TCTGTTTCTT 
CACAATGGCA AAGTCAAGGT TTTTCAGGGA AATCAAGACT CCTTCACACC CGTGGTGAAC 
TCTCTAGACC CCCCACTACT GACTCGCTAC CTTAGAATTC ACCCCCAGAG CTGGGCACAC 
CAGATCGCCC TGAGGCTGGA GGTTCTGGGC TGTGAGGCCC AGCAGGTATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004001103.1
CDS	190..7203
Translation	MQIELSTCFFLCLLPFSFSATRKYYLGAVELSWDYMQSELLSELHVDTRLSPGVPRSLPFNTSVMYRKTVFVEFTDRLFNIAKPRPPWMGLLGPTIRAEVFDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAEERTQTLHEFVLLFAVFDEGKSWHSSAQAQHEMHTVNGYVNRSLPGLTGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLIRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKTNEKEEDYDDDLYDSDMDMVRFDGDSPSPFIQIRSVAKKHPKTWIHYIAAEEEDWDYAPSAPTPIDRSYKHLYLNSGPQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLYSGRLPKGMKHLKDMPIMPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGLIGPLLICYKGSVDQRGNQMMSDKRNVILFSVFDENQSWYLAENMKRFLPNADAVPPHDPELQVSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNGNIDDYDEDRYEDIPASLLNENNVIEPRSFSQNSRHHSPRQKQFKATTTPENDIEKIDPQPGERTQLLNVQSVSSSDLLMLLGQNPTPRGLLLSDFQEATHEADDHLLGAIERHKGPSAVADLRPELHHSGDRVFTPEPKLKLRLNENLGTTVTAELKNLDLKVSSSSDNLTASPTIPSDKLAAGTEKTGSLGPPNMSIHLNSHLGTIVFGKNSSHSIDSGVPLGSSEGDNDSKLLEAALMNSQESSLGENVLSVERNGLFKEDRVHGPASLIKDNALFKVNISLIKTNNATINSTTNSKNHVDVPTLLTENSTSVWKDTILESNAEFQEMTSLLHNETFMDRNITALELKHVSNKTTSSKNVEVVYQNKEGSVPLGAENPGLSFFKTLFSPDSAKWIKWTHGENSLSSGQRPIPTQLTSLGSEKSVKDQKFLSEKKVVVGEDEFTKDTELKEMSFPNSKSMFFTNLPNVQDNGPYNQEEKSQEEIERKEKLTQENVVLPQEYTVADTNNSLKNLFLLSTKQNVEGLDEGPYTPILQDTRSLNDSAHRAGIHIVHFSKIREEENLEGLGNQTKEMAERFSSTPRMSPNPSQHNILTQRGKRALKQSRLSLEETKLARGVILNDTSTQSSKNMNFLMHGTLTQIEDNEKEKGAATQSLQSDCSMGNQGTIQMNDSALPIAKESAFPSIRHTDLTKIPSQDNSSHLPVSACTYTFRESSGVQKSTHFSQGAKRNKLSVAFLTSEMIVGQGKFSSLGKSAMNQRMSQKRENTLLQQGLSEASGKVELLPKAHVHQEDSFPTKTSNDSSGHLDLMKKIFLRKTQGPKKINGPGKVPFLKWTTESSEKIPSELLGPLAWDNNNATQIPREEWKSQKSQKNIAFKSKDTILPLGPCENSPSVAAINEGQGKLQREATRSKQGETGRLCSQNPPVSKRHQREITLTTLQSEEDKIEYDDTFSIEMKREDFDIYGEDENQGLRSFQKRTRHYFIAAVERLWDYGMSRSPHALRNRAPGGDVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKKFVNPNETRVYFWKVQHQMAPTEDEFDCKAWAYFSDVDLEKDMHSGLIGPLLICRSNTLSPAHGRQVTVQEFALFFTIFDETKSWYFTENMERSCRAPCSIQKEDPTFKEKYRFHAINGYVMDTLPGLVVAQDQKIRWYLLSMGSNENIHSIHFSGQVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKVGIWRIECLIGEHLQAGMSTLFLVYSPKCQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPISWIKVDLLAPMIIHSIMTQGARQKLSSLYISQFIIMYSLDGKKWQSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQVTASSYLNSMFGTWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFQKTMKVTGIATQGVKSLLTSMYVKEFLVSSSQDGHNWALFLHNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWAHQIALRLEVLGCEAQQVY

Target ORF information:

RefSeq Version	XM_004001054.4
Organism	Felis catus(domestic cat)
Definition	Felis catus coagulation factor VIII (F8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004001054.4

ORF Insert Sequence:

ATGCAAATAG AACTCTCCAC CTGCTTCTTT CTGTGCCTTT TGCCATTCAG CTTCAGTGCC 
ACAAGAAAAT ACTACCTGGG CGCAGTGGAA CTGTCCTGGG ACTATATGCA AAGTGAACTG 
CTCAGTGAGC TGCACGTGGA CACAAGGTTA TCCCCTGGGG TGCCAAGATC TTTGCCATTC 
AACACATCAG TCATGTACAG AAAGACTGTG TTTGTAGAGT TTACAGATCG CCTTTTCAAC 
ATTGCCAAGC CCAGGCCACC ATGGATGGGT CTGCTGGGTC CTACCATCCG GGCCGAAGTT 
TTTGACACAG TGGTCATTAC ACTCAAGAAC ATGGCTTCTC ACCCTGTCAG TCTTCATGCT 
GTTGGTGTAT CCTATTGGAA AGCTTCTGAA GGTGCTGAAT ATGAGGACCA GACCAGCCAG 
AAGGAGAAGG AAGATGATAA AGTCTTTCCT GGTGAGAGTC ATACCTACGT CTGGCAGGTC 
CTGAAAGAGA ATGGACCGAT GGCCTCGGAT CCTCCATGTC TCACCTACTC ATATTTTTCT 
CATGTGGACC TGGTGAAAGA CCTGAATTCA GGCCTCATTG GAGCCCTGCT GGTTTGCAAA 
GAAGGAAGTC TGGCTGAAGA AAGGACACAG ACCTTGCATG AATTTGTACT CCTGTTTGCT 
GTATTTGATG AAGGAAAAAG CTGGCACTCA TCTGCTCAGG CCCAGCATGA AATGCACACG 
GTCAACGGCT ATGTCAACAG GTCTCTGCCA GGTCTGACTG GATGCCACAA GAAATCAGTC 
TATTGGCATG TGATTGGAAT GGGCACCACC CCCGAAGTGC ACTCAATTTT TCTTGAAGGT 
CACACATTTC TCATCAGAAA CCACCGCCAG GCCTCCTTGG AGATCTCACC AATAACTTTC 
CTCACTGCTC AGACATTCCT GATGGACCTT GGCCAGTTTC TACTGTTTTG TCACATCTCT 
TCCCACCAGC ATGATGGCAT GGAAGCTTAT GTCAAAGTAG ATAGCTGCCC AGAGGAACCC 
CAGCTACGGA TGAAAACTAA TGAAAAAGAG GAAGATTATG ATGATGATCT TTATGATTCT 
GACATGGACA TGGTTAGGTT TGATGGCGAC AGCCCTTCTC CCTTTATTCA AATCCGCTCA 
GTTGCCAAGA AGCATCCTAA AACTTGGATC CACTATATTG CCGCCGAAGA GGAGGACTGG 
GACTATGCTC CCTCGGCCCC CACCCCCATT GACAGAAGTT ATAAACATCT ATATTTGAAC 
AGTGGTCCTC AGCGGATTGG TAGGAAGTAC AAAAAAGTTC GATTTATGGC ATACACAGAT 
GAAACATTTA AGACTCGTGA AGCTATTCAG TATGAATCAG GAATCCTAGG ACCTTTACTT 
TATGGAGAAG TTGGAGACAC ACTGTTGATT ATATTTAAAA ATCAAGCAAG CCGACCATAT 
AACATCTACC CTCATGGAAT CACTGACGTC AGTCCTTTGT ACTCAGGGAG ATTGCCTAAA 
GGTATGAAAC ATTTGAAAGA TATGCCAATT ATGCCAGGAG AGATATTCAA GTATAAATGG 
ACAGTGACTG TAGAAGATGG GCCGACTAAA TCAGATCCTC GGTGCCTGAC TCGATATTAC 
TCGAGCTTCA TTAATCCAGA GAGAGATCTA GCTTCAGGAC TCATTGGCCC TCTCCTCATA 
TGCTACAAAG GATCTGTAGA TCAAAGAGGA AACCAGATGA TGTCAGACAA GAGAAACGTC 
ATCCTGTTTT CTGTATTTGA TGAGAACCAA AGCTGGTACC TTGCAGAGAA TATGAAGCGC 
TTCCTCCCGA ATGCAGATGC AGTGCCGCCC CACGACCCGG AGCTCCAGGT CTCTAACATC 
ATGCACAGCA TCAACGGCTA TGTTTTTGAC AACTTGCAGC TGTCAGTTTG TTTGCATGAG 
GTGGCATACT GGTACATTCT AAGTGTTGGA GCACAAACTG ACTTCCTGTC TGTCTTCTTC 
TCTGGATACA CCTTCAAACA CAAAATGGTC TATGAAGACA CACTTACCCT ATTCCCATTC 
TCAGGAGAAA CTGTCTTCAT GTCAATGGAA AATCCAGGTC TATGGGTGCT GGGGTGCCAC 
AACTCAGACT TTCGGAACAG AGGCATGACA GCTTTACTGA AGGTTTCTAG TTGTAATGGG 
AACATTGATG ATTATGATGA GGATAGATAT GAAGATATTC CAGCCTCCCT CCTGAATGAA 
AACAATGTCA TTGAACCCAG AAGCTTCTCC CAGAATTCAA GACATCATAG CCCTAGGCAA 
AAGCAATTCA AAGCCACCAC AACTCCAGAA AATGACATAG AGAAGATTGA CCCTCAGCCT 
GGAGAAAGAA CGCAGCTGCT TAACGTACAA AGTGTCTCCT CTAGTGATTT GCTGATGCTT 
CTGGGACAGA ATCCTACTCC ACGTGGATTA CTCTTATCTG ATTTCCAGGA AGCCACACAT 
GAAGCAGATG ATCATTTACT TGGAGCTATA GAAAGACACA AGGGCCCATC TGCAGTGGCA 
GATCTCAGAC CAGAGCTCCA TCACAGTGGG GACAGAGTAT TTACTCCTGA GCCAAAACTA 
AAATTAAGAT TAAATGAGAA TTTGGGGACA ACTGTAACAG CAGAGTTGAA GAACCTTGAT 
TTAAAAGTTT CTAGTTCATC AGACAACCTA ACAGCTTCAC CAACAATTCC ATCAGACAAG 
TTGGCAGCAG GTACTGAAAA GACAGGTTCC TTAGGACCCC CAAATATGTC AATTCACTTA 
AATAGTCATT TAGGTACTAT TGTATTTGGC AAAAATTCAT CTCACTCTAT TGACTCTGGT 
GTACCTTTGG GCTCGAGTGA AGGAGACAAT GATTCCAAGT TGTTAGAAGC AGCTTTAATG 
AATAGTCAAG AAAGTTCACT GGGAGAAAAT GTATTATCAG TGGAGAGAAA TGGGTTATTT 
AAAGAAGACA GAGTTCATGG ACCTGCTTCA TTAATTAAAG ATAATGCTTT ATTCAAAGTT 
AATATCTCTT TGATAAAGAC AAACAACGCA ACAATTAACT CAACAACTAA TAGCAAAAAT 
CATGTTGATG TCCCAACATT ATTAACTGAG AACAGTACAT CAGTCTGGAA AGATACTATA 
TTAGAAAGTA ATGCTGAGTT TCAAGAAATG ACTTCTTTGC TTCATAATGA AACATTTATG 
GACAGAAATA TTACAGCTTT GGAGCTAAAG CATGTGTCAA ATAAAACTAC TTCATCAAAA 
AATGTGGAAG TGGTCTACCA AAACAAAGAG GGCTCGGTGC CACTAGGTGC AGAAAATCCA 
GGTCTCTCAT TCTTCAAGAC ACTGTTCTCA CCAGATTCAG CAAAGTGGAT AAAATGGACC 
CATGGCGAGA ACTCCCTAAG CTCTGGGCAA AGGCCCATTC CAACACAATT AACATCTTTA 
GGATCAGAAA AATCTGTGAA AGATCAGAAG TTTTTGTCAG AAAAGAAGGT GGTAGTAGGA 
GAAGATGAAT TTACAAAGGA CACAGAACTC AAAGAGATGA GTTTTCCAAA CAGCAAGAGC 
ATGTTTTTTA CTAACTTGCC CAATGTCCAA GATAATGGTC CTTACAATCA AGAAGAAAAA 
TCTCAGGAAG AGATAGAAAG AAAGGAGAAA TTAACCCAAG AAAATGTGGT TTTGCCTCAG 
GAATATACAG TAGCTGACAC TAATAATTCC CTGAAGAACC TTTTCTTACT AAGCACTAAG 
CAAAATGTGG AAGGTTTAGA TGAGGGGCCA TATACTCCAA TACTTCAAGA CACCAGGTCA 
TTAAATGATT CAGCACATAG AGCAGGGATT CACATAGTCC ATTTCTCAAA AATAAGGGAG 
GAAGAAAACT TGGAAGGTTT GGGAAATCAA ACAAAGGAAA TGGCAGAGAG GTTTTCAAGC 
ACTCCAAGGA TGTCTCCTAA TCCAAGTCAG CATAATATTC TCACTCAGCG TGGGAAGCGG 
GCCTTGAAAC AATCCAGACT CTCACTAGAA GAAACTAAGC TTGCAAGGGG GGTAATTTTG 
AATGACACCT CAACCCAGTC ATCCAAAAAC ATGAACTTTT TGATGCATGG AACCCTCACA 
CAGATAGAGG ACAATGAGAA AGAAAAAGGG GCTGCCACTC AGTCCCTCCA ATCAGATTGT 
TCTATGGGGA ATCAGGGCAC CATTCAAATG AATGACTCTG CATTACCCAT TGCAAAGGAA 
TCAGCATTTC CATCAATCAG ACATACAGAT CTGACCAAGA TCCCATCCCA GGACAACTCT 
TCTCATCTTC CAGTATCAGC CTGCACTTAT ACCTTCAGGG AGAGTTCTGG GGTCCAAAAA 
AGCACTCATT TCTCACAAGG AGCCAAGAGA AATAAACTTT CTGTAGCCTT CCTAACCTCA 
GAAATGATTG TAGGTCAAGG AAAGTTCAGC TCCCTAGGGA AAAGTGCCAT GAACCAACGC 
ATGTCCCAGA AACGTGAAAA CACTCTTTTG CAACAAGGCT TGTCTGAAGC ATCTGGCAAA 
GTTGAATTAC TCCCTAAAGC TCATGTTCAT CAAGAAGACT CTTTCCCTAC AAAAACTAGC 
AATGATTCTT CTGGCCACCT GGATCTTATG AAGAAGATCT TCCTTCGGAA AACACAGGGA 
CCTAAGAAAA TAAATGGACC CGGAAAAGTG CCCTTCCTGA AATGGACAAC AGAGAGCTCT 
GAAAAGATTC CCTCTGAGCT GCTGGGTCCC CTTGCTTGGG ATAACAACAA TGCCACCCAG 
ATACCGAGAG AAGAGTGGAA ATCCCAAAAG TCACAGAAAA ACATAGCTTT TAAGAGCAAG 
GACACCATTT TGCCCTTGGG CCCTTGTGAA AACAGTCCTT CAGTAGCAGC AATAAATGAA 
GGGCAAGGTA AACTCCAAAG AGAAGCCACC AGGTCAAAGC AAGGAGAGAC TGGAAGATTG 
TGCTCTCAGA ACCCACCGGT CTCAAAACGC CATCAAAGAG AAATAACCCT TACTACTCTT 
CAGTCAGAGG AAGACAAAAT TGAGTATGAT GATACGTTTT CAATTGAAAT GAAGAGAGAA 
GATTTTGACA TCTATGGTGA AGATGAAAAT CAGGGCCTCC GCAGCTTTCA AAAGAGAACA 
CGACACTATT TCATTGCTGC AGTGGAGCGT CTCTGGGATT ATGGGATGAG TAGATCTCCC 
CATGCACTAA GAAACAGGGC TCCAGGTGGG GATGTCCCTC AGTTCAAGAA GGTGGTTTTC 
CAGGAATTTA CTGATGGATC CTTCACTCAG CCCTTATACC GTGGAGAGCT GAATGAACAT 
TTGGGACTCT TGGGGCCATA TATAAGAGCA GAAGTTGAAG ACAATATCAT GGTAACTTTC 
AAAAACCAGG CTTCTCGTCC CTACTCCTTC TATTCTAGCC TGATTTCTTA TGAGGAAGAT 
CAGAGGCAAG GAGCAGAACC TAGAAAAAAG TTTGTCAACC CTAATGAAAC CAGAGTTTAC 
TTCTGGAAAG TACAACATCA GATGGCACCC ACTGAAGATG AGTTTGACTG CAAGGCTTGG 
GCTTATTTTT CTGATGTTGA TTTGGAAAAA GACATGCACT CGGGCTTGAT TGGACCCCTT 
CTGATCTGCC GCAGTAACAC ACTGAGCCCC GCTCACGGGA GACAAGTGAC AGTGCAGGAG 
TTTGCTCTGT TTTTCACCAT TTTTGATGAG ACTAAGAGCT GGTACTTCAC GGAAAACATG 
GAAAGGAGCT GTAGAGCCCC CTGCAGTATC CAGAAGGAGG ATCCCACTTT TAAAGAAAAG 
TACCGCTTCC ACGCAATCAA CGGCTATGTG ATGGACACAC TACCCGGCTT GGTAGTGGCT 
CAGGATCAGA AGATCCGATG GTATCTGCTC AGCATGGGGA GCAATGAAAA CATTCATTCT 
ATTCATTTCA GTGGGCAGGT GTTCACTGTA CGGAAAAAAG AGGAATATAA GATGGCAGTC 
TACAACCTGT ATCCAGGTGT TTTTGAGACA GTGGAAATGC TACCATCCAA AGTTGGAATT 
TGGCGGATAG AATGCCTAAT TGGCGAACAC CTGCAAGCTG GGATGAGCAC TCTCTTTCTG 
GTATACAGCC CGAAGTGTCA GATTCCACTG GGAATGGCTT CTGGACGCAT TAGAGATTTC 
CAGATCACAG CTTCAGGACA GTATGGACAG TGGGCCCCAA AGCTGGCCAG ACTTCACTAT 
TCTGGATCAA TCAATGCCTG GAGCACCAAG GACCCCATTT CCTGGATCAA GGTGGATCTG 
TTGGCACCAA TGATCATTCA CAGCATCATG ACCCAGGGCG CCCGTCAGAA GTTGTCCAGC 
CTGTACATCT CTCAGTTTAT CATCATGTAT AGTCTTGATG GAAAGAAGTG GCAGAGTTAT 
CGAGGGAATT CCACTGGGAC CTTAATGGTG TTCTTTGGCA ATGTGGATTC ATCTGGGATA 
AAACACAATA TTTTTAACCC TCCGATTATT GCTCGGTACA TCCGTTTGCA CCCAACGCAT 
TACAGCATCC GCAGCACTCT CCGCATGGAG TTGATGGGCT GTGATTTAAA CAGCTGCAGC 
ATGCCACTGG GAATGGAGAG TAAAGCAATA TCAGACGCAC AGGTCACCGC CTCGTCCTAC 
TTAAACAGCA TGTTTGGCAC TTGGTCCCCT TCACAAGCCC GGCTGCACCT CCAGGGGAGG 
ACGAATGCCT GGAGGCCTCA GGCAAATAAT CCAAAAGAGT GGCTGCAAGT GGACTTCCAG 
AAGACAATGA AAGTCACAGG AATAGCCACC CAGGGGGTGA AATCACTCCT TACCAGCATG 
TATGTGAAGG AGTTCCTCGT TTCCAGTAGT CAAGATGGCC ATAACTGGGC TCTGTTTCTT 
CACAATGGCA AAGTCAAGGT TTTTCAGGGA AATCAAGACT CCTTCACACC CGTGGTGAAC 
TCTCTAGACC CCCCACTACT GACTCGCTAC CTTAGAATTC ACCCCCAGAG CTGGGCACAC 
CAGATCGCCC TGAGGCTGGA GGTTCTGGGC TGTGAGGCCC AGCAGGTATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_019824515.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019680074.1 Felis catus coagulation factor VIII (F8), transcript variant X2, mRNA.

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CloneID	OFb58139
Clone ID Related Accession (Same CDS sequence)	XM_019824515.1 , XM_019824516.1 , XM_019824515.2
Accession Version	XM_019824515.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	coagulation factor VIII isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGTG AACTGCTCAG TGAGCTGCAC GTGGACACAA GGTTATCCCC TGGGGTGCCA 
AGATCTTTGC CATTCAACAC ATCAGTCATG TACAGAAAGA CTGTGTTTGT AGAGTTTACA 
GATCGCCTTT TCAACATTGC CAAGCCCAGG CCACCATGGA TGGGTCTGCT GGGTCCTACC 
ATCCGGGCCG AAGTTTTTGA CACAGTGGTC ATTACACTCA AGAACATGGC TTCTCACCCT 
GTCAGTCTTC ATGCTGTTGG TGTATCCTAT TGGAAAGCTT CTGAAGGTGC TGAATATGAG 
GACCAGACCA GCCAGAAGGA GAAGGAAGAT GATAAAGTCT TTCCTGGTGA GAGTCATACC 
TACGTCTGGC AGGTCCTGAA AGAGAATGGA CCGATGGCCT CGGATCCTCC ATGTCTCACC 
TACTCATATT TTTCTCATGT GGACCTGGTG AAAGACCTGA ATTCAGGCCT CATTGGAGCC 
CTGCTGGTTT GCAAAGAAGG AAGTCTGGCT GAAGAAAGGA CACAGACCTT GCATGAATTT 
GTACTCCTGT TTGCTGTATT TGATGAAGGA AAAAGCTGGC ACTCATCTGC TCAGGCCCAG 
CATGAAATGC ACACGGTCAA CGGCTATGTC AACAGGTCTC TGCCAGGTCT GACTGGATGC 
CACAAGAAAT CAGTCTATTG GCATGTGATT GGAATGGGCA CCACCCCCGA AGTGCACTCA 
ATTTTTCTTG AAGGTCACAC ATTTCTCATC AGAAACCACC GCCAGGCCTC CTTGGAGATC 
TCACCAATAA CTTTCCTCAC TGCTCAGACA TTCCTGATGG ACCTTGGCCA GTTTCTACTG 
TTTTGTCACA TCTCTTCCCA CCAGCATGAT GGCATGGAAG CTTATGTCAA AGTAGATAGC 
TGCCCAGAGG AACCCCAGCT ACGGATGAAA ACTAATGAAA AAGAGGAAGA TTATGATGAT 
GATCTTTATG ATTCTGACAT GGACATGGTT AGGTTTGATG GCGACAGCCC TTCTCCCTTT 
ATTCAAATCC GCTCAGTTGC CAAGAAGCAT CCTAAAACTT GGATCCACTA TATTGCCGCC 
GAAGAGGAGG ACTGGGACTA TGCTCCCTCG GCCCCCACCC CCATTGACAG AAGTTATAAA 
CATCTATATT TGAACAGTGG TCCTCAGCGG ATTGGTAGGA AGTACAAAAA AGTTCGATTT 
ATGGCATACA CAGATGAAAC ATTTAAGACT CGTGAAGCTA TTCAGTATGA ATCAGGAATC 
CTAGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATATT TAAAAATCAA 
GCAAGCCGAC CATATAACAT CTACCCTCAT GGAATCACTG ACGTCAGTCC TTTGTACTCA 
GGGAGATTGC CTAAAGGTAT GAAACATTTG AAAGATATGC CAATTATGCC AGGAGAGATA 
TTCAAGTATA AATGGACAGT GACTGTAGAA GATGGGCCGA CTAAATCAGA TCCTCGGTGC 
CTGACTCGAT ATTACTCGAG CTTCATTAAT CCAGAGAGAG ATCTAGCTTC AGGACTCATT 
GGCCCTCTCC TCATATGCTA CAAAGGATCT GTAGATCAAA GAGGAAACCA GATGATGTCA 
GACAAGAGAA ACGTCATCCT GTTTTCTGTA TTTGATGAGA ACCAAAGCTG GTACCTTGCA 
GAGAATATGA AGCGCTTCCT CCCGAATGCA GATGCAGTGC CGCCCCACGA CCCGGAGCTC 
CAGGTCTCTA ACATCATGCA CAGCATCAAC GGCTATGTTT TTGACAACTT GCAGCTGTCA 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCACA AACTGACTTC 
CTGTCTGTCT TCTTCTCTGG ATACACCTTC AAACACAAAA TGGTCTATGA AGACACACTT 
ACCCTATTCC CATTCTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTATGG 
GTGCTGGGGT GCCACAACTC AGACTTTCGG AACAGAGGCA TGACAGCTTT ACTGAAGGTT 
TCTAGTTGTA ATGGGAACAT TGATGATTAT GATGAGGATA GATATGAAGA TATTCCAGCC 
TCCCTCCTGA ATGAAAACAA TGTCATTGAA CCCAGAAGCT TCTCCCAGAA TTCAAGACAT 
CATAGCCCTA GGCAAAAGCA ATTCAAAGCC ACCACAACTC CAGAAAATGA CATAGAGAAG 
ATTGACCCTC AGCCTGGAGA AAGAACGCAG CTGCTTAACG TACAAAGTGT CTCCTCTAGT 
GATTTGCTGA TGCTTCTGGG ACAGAATCCT ACTCCACGTG GATTACTCTT ATCTGATTTC 
CAGGAAGCCA CACATGAAGC AGATGATCAT TTACTTGGAG CTATAGAAAG ACACAAGGGC 
CCATCTGCAG TGGCAGATCT CAGACCAGAG CTCCATCACA GTGGGGACAG AGTATTTACT 
CCTGAGCCAA AACTAAAATT AAGATTAAAT GAGAATTTGG GGACAACTGT AACAGCAGAG 
TTGAAGAACC TTGATTTAAA AGTTTCTAGT TCATCAGACA ACCTAACAGC TTCACCAACA 
ATTCCATCAG ACAAGTTGGC AGCAGGTACT GAAAAGACAG GTTCCTTAGG ACCCCCAAAT 
ATGTCAATTC ACTTAAATAG TCATTTAGGT ACTATTGTAT TTGGCAAAAA TTCATCTCAC 
TCTATTGACT CTGGTGTACC TTTGGGCTCG AGTGAAGGAG ACAATGATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGTATT ATCAGTGGAG 
AGAAATGGGT TATTTAAAGA AGACAGAGTT CATGGACCTG CTTCATTAAT TAAAGATAAT 
GCTTTATTCA AAGTTAATAT CTCTTTGATA AAGACAAACA ACGCAACAAT TAACTCAACA 
ACTAATAGCA AAAATCATGT TGATGTCCCA ACATTATTAA CTGAGAACAG TACATCAGTC 
TGGAAAGATA CTATATTAGA AAGTAATGCT GAGTTTCAAG AAATGACTTC TTTGCTTCAT 
AATGAAACAT TTATGGACAG AAATATTACA GCTTTGGAGC TAAAGCATGT GTCAAATAAA 
ACTACTTCAT CAAAAAATGT GGAAGTGGTC TACCAAAACA AAGAGGGCTC GGTGCCACTA 
GGTGCAGAAA ATCCAGGTCT CTCATTCTTC AAGACACTGT TCTCACCAGA TTCAGCAAAG 
TGGATAAAAT GGACCCATGG CGAGAACTCC CTAAGCTCTG GGCAAAGGCC CATTCCAACA 
CAATTAACAT CTTTAGGATC AGAAAAATCT GTGAAAGATC AGAAGTTTTT GTCAGAAAAG 
AAGGTGGTAG TAGGAGAAGA TGAATTTACA AAGGACACAG AACTCAAAGA GATGAGTTTT 
CCAAACAGCA AGAGCATGTT TTTTACTAAC TTGCCCAATG TCCAAGATAA TGGTCCTTAC 
AATCAAGAAG AAAAATCTCA GGAAGAGATA GAAAGAAAGG AGAAATTAAC CCAAGAAAAT 
GTGGTTTTGC CTCAGGAATA TACAGTAGCT GACACTAATA ATTCCCTGAA GAACCTTTTC 
TTACTAAGCA CTAAGCAAAA TGTGGAAGGT TTAGATGAGG GGCCATATAC TCCAATACTT 
CAAGACACCA GGTCATTAAA TGATTCAGCA CATAGAGCAG GGATTCACAT AGTCCATTTC 
TCAAAAATAA GGGAGGAAGA AAACTTGGAA GGTTTGGGAA ATCAAACAAA GGAAATGGCA 
GAGAGGTTTT CAAGCACTCC AAGGATGTCT CCTAATCCAA GTCAGCATAA TATTCTCACT 
CAGCGTGGGA AGCGGGCCTT GAAACAATCC AGACTCTCAC TAGAAGAAAC TAAGCTTGCA 
AGGGGGGTAA TTTTGAATGA CACCTCAACC CAGTCATCCA AAAACATGAA CTTTTTGATG 
CATGGAACCC TCACACAGAT AGAGGACAAT GAGAAAGAAA AAGGGGCTGC CACTCAGTCC 
CTCCAATCAG ATTGTTCTAT GGGGAATCAG GGCACCATTC AAATGAATGA CTCTGCATTA 
CCCATTGCAA AGGAATCAGC ATTTCCATCA ATCAGACATA CAGATCTGAC CAAGATCCCA 
TCCCAGGACA ACTCTTCTCA TCTTCCAGTA TCAGCCTGCA CTTATACCTT CAGGGAGAGT 
TCTGGGGTCC AAAAAAGCAC TCATTTCTCA CAAGGAGCCA AGAGAAATAA ACTTTCTGTA 
GCCTTCCTAA CCTCAGAAAT GATTGTAGGT CAAGGAAAGT TCAGCTCCCT AGGGAAAAGT 
GCCATGAACC AACGCATGTC CCAGAAACGT GAAAACACTC TTTTGCAACA AGGCTTGTCT 
GAAGCATCTG GCAAAGTTGA ATTACTCCCT AAAGCTCATG TTCATCAAGA AGACTCTTTC 
CCTACAAAAA CTAGCAATGA TTCTTCTGGC CACCTGGATC TTATGAAGAA GATCTTCCTT 
CGGAAAACAC AGGGACCTAA GAAAATAAAT GGACCCGGAA AAGTGCCCTT CCTGAAATGG 
ACAACAGAGA GCTCTGAAAA GATTCCCTCT GAGCTGCTGG GTCCCCTTGC TTGGGATAAC 
AACAATGCCA CCCAGATACC GAGAGAAGAG TGGAAATCCC AAAAGTCACA GAAAAACATA 
GCTTTTAAGA GCAAGGACAC CATTTTGCCC TTGGGCCCTT GTGAAAACAG TCCTTCAGTA 
GCAGCAATAA ATGAAGGGCA AGGTAAACTC CAAAGAGAAG CCACCAGGTC AAAGCAAGGA 
GAGACTGGAA GATTGTGCTC TCAGAACCCA CCGGTCTCAA AACGCCATCA AAGAGAAATA 
ACCCTTACTA CTCTTCAGTC AGAGGAAGAC AAAATTGAGT ATGATGATAC GTTTTCAATT 
GAAATGAAGA GAGAAGATTT TGACATCTAT GGTGAAGATG AAAATCAGGG CCTCCGCAGC 
TTTCAAAAGA GAACACGACA CTATTTCATT GCTGCAGTGG AGCGTCTCTG GGATTATGGG 
ATGAGTAGAT CTCCCCATGC ACTAAGAAAC AGGGCTCCAG GTGGGGATGT CCCTCAGTTC 
AAGAAGGTGG TTTTCCAGGA ATTTACTGAT GGATCCTTCA CTCAGCCCTT ATACCGTGGA 
GAGCTGAATG AACATTTGGG ACTCTTGGGG CCATATATAA GAGCAGAAGT TGAAGACAAT 
ATCATGGTAA CTTTCAAAAA CCAGGCTTCT CGTCCCTACT CCTTCTATTC TAGCCTGATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGAA AAAAGTTTGT CAACCCTAAT 
GAAACCAGAG TTTACTTCTG GAAAGTACAA CATCAGATGG CACCCACTGA AGATGAGTTT 
GACTGCAAGG CTTGGGCTTA TTTTTCTGAT GTTGATTTGG AAAAAGACAT GCACTCGGGC 
TTGATTGGAC CCCTTCTGAT CTGCCGCAGT AACACACTGA GCCCCGCTCA CGGGAGACAA 
GTGACAGTGC AGGAGTTTGC TCTGTTTTTC ACCATTTTTG ATGAGACTAA GAGCTGGTAC 
TTCACGGAAA ACATGGAAAG GAGCTGTAGA GCCCCCTGCA GTATCCAGAA GGAGGATCCC 
ACTTTTAAAG AAAAGTACCG CTTCCACGCA ATCAACGGCT ATGTGATGGA CACACTACCC 
GGCTTGGTAG TGGCTCAGGA TCAGAAGATC CGATGGTATC TGCTCAGCAT GGGGAGCAAT 
GAAAACATTC ATTCTATTCA TTTCAGTGGG CAGGTGTTCA CTGTACGGAA AAAAGAGGAA 
TATAAGATGG CAGTCTACAA CCTGTATCCA GGTGTTTTTG AGACAGTGGA AATGCTACCA 
TCCAAAGTTG GAATTTGGCG GATAGAATGC CTAATTGGCG AACACCTGCA AGCTGGGATG 
AGCACTCTCT TTCTGGTATA CAGCCCGAAG TGTCAGATTC CACTGGGAAT GGCTTCTGGA 
CGCATTAGAG ATTTCCAGAT CACAGCTTCA GGACAGTATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ACTATTCTGG ATCAATCAAT GCCTGGAGCA CCAAGGACCC CATTTCCTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATC ATTCACAGCA TCATGACCCA GGGCGCCCGT 
CAGAAGTTGT CCAGCCTGTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGAAAG 
AAGTGGCAGA GTTATCGAGG GAATTCCACT GGGACCTTAA TGGTGTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCGA TTATTGCTCG GTACATCCGT 
TTGCACCCAA CGCATTACAG CATCCGCAGC ACTCTCCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAACAGCT GCAGCATGCC ACTGGGAATG GAGAGTAAAG CAATATCAGA CGCACAGGTC 
ACCGCCTCGT CCTACTTAAA CAGCATGTTT GGCACTTGGT CCCCTTCACA AGCCCGGCTG 
CACCTCCAGG GGAGGACGAA TGCCTGGAGG CCTCAGGCAA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAG CCACCCAGGG GGTGAAATCA 
CTCCTTACCA GCATGTATGT GAAGGAGTTC CTCGTTTCCA GTAGTCAAGA TGGCCATAAC 
TGGGCTCTGT TTCTTCACAA TGGCAAAGTC AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCCGTGG TGAACTCTCT AGACCCCCCA CTACTGACTC GCTACCTTAG AATTCACCCC 
CAGAGCTGGG CACACCAGAT CGCCCTGAGG CTGGAGGTTC TGGGCTGTGA GGCCCAGCAG 
GTATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019680074.1
CDS	611..7519
Translation	MQSELLSELHVDTRLSPGVPRSLPFNTSVMYRKTVFVEFTDRLFNIAKPRPPWMGLLGPTIRAEVFDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAEERTQTLHEFVLLFAVFDEGKSWHSSAQAQHEMHTVNGYVNRSLPGLTGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLIRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKTNEKEEDYDDDLYDSDMDMVRFDGDSPSPFIQIRSVAKKHPKTWIHYIAAEEEDWDYAPSAPTPIDRSYKHLYLNSGPQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLYSGRLPKGMKHLKDMPIMPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGLIGPLLICYKGSVDQRGNQMMSDKRNVILFSVFDENQSWYLAENMKRFLPNADAVPPHDPELQVSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNGNIDDYDEDRYEDIPASLLNENNVIEPRSFSQNSRHHSPRQKQFKATTTPENDIEKIDPQPGERTQLLNVQSVSSSDLLMLLGQNPTPRGLLLSDFQEATHEADDHLLGAIERHKGPSAVADLRPELHHSGDRVFTPEPKLKLRLNENLGTTVTAELKNLDLKVSSSSDNLTASPTIPSDKLAAGTEKTGSLGPPNMSIHLNSHLGTIVFGKNSSHSIDSGVPLGSSEGDNDSKLLEAALMNSQESSLGENVLSVERNGLFKEDRVHGPASLIKDNALFKVNISLIKTNNATINSTTNSKNHVDVPTLLTENSTSVWKDTILESNAEFQEMTSLLHNETFMDRNITALELKHVSNKTTSSKNVEVVYQNKEGSVPLGAENPGLSFFKTLFSPDSAKWIKWTHGENSLSSGQRPIPTQLTSLGSEKSVKDQKFLSEKKVVVGEDEFTKDTELKEMSFPNSKSMFFTNLPNVQDNGPYNQEEKSQEEIERKEKLTQENVVLPQEYTVADTNNSLKNLFLLSTKQNVEGLDEGPYTPILQDTRSLNDSAHRAGIHIVHFSKIREEENLEGLGNQTKEMAERFSSTPRMSPNPSQHNILTQRGKRALKQSRLSLEETKLARGVILNDTSTQSSKNMNFLMHGTLTQIEDNEKEKGAATQSLQSDCSMGNQGTIQMNDSALPIAKESAFPSIRHTDLTKIPSQDNSSHLPVSACTYTFRESSGVQKSTHFSQGAKRNKLSVAFLTSEMIVGQGKFSSLGKSAMNQRMSQKRENTLLQQGLSEASGKVELLPKAHVHQEDSFPTKTSNDSSGHLDLMKKIFLRKTQGPKKINGPGKVPFLKWTTESSEKIPSELLGPLAWDNNNATQIPREEWKSQKSQKNIAFKSKDTILPLGPCENSPSVAAINEGQGKLQREATRSKQGETGRLCSQNPPVSKRHQREITLTTLQSEEDKIEYDDTFSIEMKREDFDIYGEDENQGLRSFQKRTRHYFIAAVERLWDYGMSRSPHALRNRAPGGDVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKKFVNPNETRVYFWKVQHQMAPTEDEFDCKAWAYFSDVDLEKDMHSGLIGPLLICRSNTLSPAHGRQVTVQEFALFFTIFDETKSWYFTENMERSCRAPCSIQKEDPTFKEKYRFHAINGYVMDTLPGLVVAQDQKIRWYLLSMGSNENIHSIHFSGQVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKVGIWRIECLIGEHLQAGMSTLFLVYSPKCQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPISWIKVDLLAPMIIHSIMTQGARQKLSSLYISQFIIMYSLDGKKWQSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQVTASSYLNSMFGTWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFQKTMKVTGIATQGVKSLLTSMYVKEFLVSSSQDGHNWALFLHNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWAHQIALRLEVLGCEAQQVY

Target ORF information:

RefSeq Version	XM_019824515.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus coagulation factor VIII (F8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019824515.1

ORF Insert Sequence:

ATGCAAAGTG AACTGCTCAG TGAGCTGCAC GTGGACACAA GGTTATCCCC TGGGGTGCCA 
AGATCTTTGC CATTCAACAC ATCAGTCATG TACAGAAAGA CTGTGTTTGT AGAGTTTACA 
GATCGCCTTT TCAACATTGC CAAGCCCAGG CCACCATGGA TGGGTCTGCT GGGTCCTACC 
ATCCGGGCCG AAGTTTTTGA CACAGTGGTC ATTACACTCA AGAACATGGC TTCTCACCCT 
GTCAGTCTTC ATGCTGTTGG TGTATCCTAT TGGAAAGCTT CTGAAGGTGC TGAATATGAG 
GACCAGACCA GCCAGAAGGA GAAGGAAGAT GATAAAGTCT TTCCTGGTGA GAGTCATACC 
TACGTCTGGC AGGTCCTGAA AGAGAATGGA CCGATGGCCT CGGATCCTCC ATGTCTCACC 
TACTCATATT TTTCTCATGT GGACCTGGTG AAAGACCTGA ATTCAGGCCT CATTGGAGCC 
CTGCTGGTTT GCAAAGAAGG AAGTCTGGCT GAAGAAAGGA CACAGACCTT GCATGAATTT 
GTACTCCTGT TTGCTGTATT TGATGAAGGA AAAAGCTGGC ACTCATCTGC TCAGGCCCAG 
CATGAAATGC ACACGGTCAA CGGCTATGTC AACAGGTCTC TGCCAGGTCT GACTGGATGC 
CACAAGAAAT CAGTCTATTG GCATGTGATT GGAATGGGCA CCACCCCCGA AGTGCACTCA 
ATTTTTCTTG AAGGTCACAC ATTTCTCATC AGAAACCACC GCCAGGCCTC CTTGGAGATC 
TCACCAATAA CTTTCCTCAC TGCTCAGACA TTCCTGATGG ACCTTGGCCA GTTTCTACTG 
TTTTGTCACA TCTCTTCCCA CCAGCATGAT GGCATGGAAG CTTATGTCAA AGTAGATAGC 
TGCCCAGAGG AACCCCAGCT ACGGATGAAA ACTAATGAAA AAGAGGAAGA TTATGATGAT 
GATCTTTATG ATTCTGACAT GGACATGGTT AGGTTTGATG GCGACAGCCC TTCTCCCTTT 
ATTCAAATCC GCTCAGTTGC CAAGAAGCAT CCTAAAACTT GGATCCACTA TATTGCCGCC 
GAAGAGGAGG ACTGGGACTA TGCTCCCTCG GCCCCCACCC CCATTGACAG AAGTTATAAA 
CATCTATATT TGAACAGTGG TCCTCAGCGG ATTGGTAGGA AGTACAAAAA AGTTCGATTT 
ATGGCATACA CAGATGAAAC ATTTAAGACT CGTGAAGCTA TTCAGTATGA ATCAGGAATC 
CTAGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATATT TAAAAATCAA 
GCAAGCCGAC CATATAACAT CTACCCTCAT GGAATCACTG ACGTCAGTCC TTTGTACTCA 
GGGAGATTGC CTAAAGGTAT GAAACATTTG AAAGATATGC CAATTATGCC AGGAGAGATA 
TTCAAGTATA AATGGACAGT GACTGTAGAA GATGGGCCGA CTAAATCAGA TCCTCGGTGC 
CTGACTCGAT ATTACTCGAG CTTCATTAAT CCAGAGAGAG ATCTAGCTTC AGGACTCATT 
GGCCCTCTCC TCATATGCTA CAAAGGATCT GTAGATCAAA GAGGAAACCA GATGATGTCA 
GACAAGAGAA ACGTCATCCT GTTTTCTGTA TTTGATGAGA ACCAAAGCTG GTACCTTGCA 
GAGAATATGA AGCGCTTCCT CCCGAATGCA GATGCAGTGC CGCCCCACGA CCCGGAGCTC 
CAGGTCTCTA ACATCATGCA CAGCATCAAC GGCTATGTTT TTGACAACTT GCAGCTGTCA 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCACA AACTGACTTC 
CTGTCTGTCT TCTTCTCTGG ATACACCTTC AAACACAAAA TGGTCTATGA AGACACACTT 
ACCCTATTCC CATTCTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTATGG 
GTGCTGGGGT GCCACAACTC AGACTTTCGG AACAGAGGCA TGACAGCTTT ACTGAAGGTT 
TCTAGTTGTA ATGGGAACAT TGATGATTAT GATGAGGATA GATATGAAGA TATTCCAGCC 
TCCCTCCTGA ATGAAAACAA TGTCATTGAA CCCAGAAGCT TCTCCCAGAA TTCAAGACAT 
CATAGCCCTA GGCAAAAGCA ATTCAAAGCC ACCACAACTC CAGAAAATGA CATAGAGAAG 
ATTGACCCTC AGCCTGGAGA AAGAACGCAG CTGCTTAACG TACAAAGTGT CTCCTCTAGT 
GATTTGCTGA TGCTTCTGGG ACAGAATCCT ACTCCACGTG GATTACTCTT ATCTGATTTC 
CAGGAAGCCA CACATGAAGC AGATGATCAT TTACTTGGAG CTATAGAAAG ACACAAGGGC 
CCATCTGCAG TGGCAGATCT CAGACCAGAG CTCCATCACA GTGGGGACAG AGTATTTACT 
CCTGAGCCAA AACTAAAATT AAGATTAAAT GAGAATTTGG GGACAACTGT AACAGCAGAG 
TTGAAGAACC TTGATTTAAA AGTTTCTAGT TCATCAGACA ACCTAACAGC TTCACCAACA 
ATTCCATCAG ACAAGTTGGC AGCAGGTACT GAAAAGACAG GTTCCTTAGG ACCCCCAAAT 
ATGTCAATTC ACTTAAATAG TCATTTAGGT ACTATTGTAT TTGGCAAAAA TTCATCTCAC 
TCTATTGACT CTGGTGTACC TTTGGGCTCG AGTGAAGGAG ACAATGATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGTATT ATCAGTGGAG 
AGAAATGGGT TATTTAAAGA AGACAGAGTT CATGGACCTG CTTCATTAAT TAAAGATAAT 
GCTTTATTCA AAGTTAATAT CTCTTTGATA AAGACAAACA ACGCAACAAT TAACTCAACA 
ACTAATAGCA AAAATCATGT TGATGTCCCA ACATTATTAA CTGAGAACAG TACATCAGTC 
TGGAAAGATA CTATATTAGA AAGTAATGCT GAGTTTCAAG AAATGACTTC TTTGCTTCAT 
AATGAAACAT TTATGGACAG AAATATTACA GCTTTGGAGC TAAAGCATGT GTCAAATAAA 
ACTACTTCAT CAAAAAATGT GGAAGTGGTC TACCAAAACA AAGAGGGCTC GGTGCCACTA 
GGTGCAGAAA ATCCAGGTCT CTCATTCTTC AAGACACTGT TCTCACCAGA TTCAGCAAAG 
TGGATAAAAT GGACCCATGG CGAGAACTCC CTAAGCTCTG GGCAAAGGCC CATTCCAACA 
CAATTAACAT CTTTAGGATC AGAAAAATCT GTGAAAGATC AGAAGTTTTT GTCAGAAAAG 
AAGGTGGTAG TAGGAGAAGA TGAATTTACA AAGGACACAG AACTCAAAGA GATGAGTTTT 
CCAAACAGCA AGAGCATGTT TTTTACTAAC TTGCCCAATG TCCAAGATAA TGGTCCTTAC 
AATCAAGAAG AAAAATCTCA GGAAGAGATA GAAAGAAAGG AGAAATTAAC CCAAGAAAAT 
GTGGTTTTGC CTCAGGAATA TACAGTAGCT GACACTAATA ATTCCCTGAA GAACCTTTTC 
TTACTAAGCA CTAAGCAAAA TGTGGAAGGT TTAGATGAGG GGCCATATAC TCCAATACTT 
CAAGACACCA GGTCATTAAA TGATTCAGCA CATAGAGCAG GGATTCACAT AGTCCATTTC 
TCAAAAATAA GGGAGGAAGA AAACTTGGAA GGTTTGGGAA ATCAAACAAA GGAAATGGCA 
GAGAGGTTTT CAAGCACTCC AAGGATGTCT CCTAATCCAA GTCAGCATAA TATTCTCACT 
CAGCGTGGGA AGCGGGCCTT GAAACAATCC AGACTCTCAC TAGAAGAAAC TAAGCTTGCA 
AGGGGGGTAA TTTTGAATGA CACCTCAACC CAGTCATCCA AAAACATGAA CTTTTTGATG 
CATGGAACCC TCACACAGAT AGAGGACAAT GAGAAAGAAA AAGGGGCTGC CACTCAGTCC 
CTCCAATCAG ATTGTTCTAT GGGGAATCAG GGCACCATTC AAATGAATGA CTCTGCATTA 
CCCATTGCAA AGGAATCAGC ATTTCCATCA ATCAGACATA CAGATCTGAC CAAGATCCCA 
TCCCAGGACA ACTCTTCTCA TCTTCCAGTA TCAGCCTGCA CTTATACCTT CAGGGAGAGT 
TCTGGGGTCC AAAAAAGCAC TCATTTCTCA CAAGGAGCCA AGAGAAATAA ACTTTCTGTA 
GCCTTCCTAA CCTCAGAAAT GATTGTAGGT CAAGGAAAGT TCAGCTCCCT AGGGAAAAGT 
GCCATGAACC AACGCATGTC CCAGAAACGT GAAAACACTC TTTTGCAACA AGGCTTGTCT 
GAAGCATCTG GCAAAGTTGA ATTACTCCCT AAAGCTCATG TTCATCAAGA AGACTCTTTC 
CCTACAAAAA CTAGCAATGA TTCTTCTGGC CACCTGGATC TTATGAAGAA GATCTTCCTT 
CGGAAAACAC AGGGACCTAA GAAAATAAAT GGACCCGGAA AAGTGCCCTT CCTGAAATGG 
ACAACAGAGA GCTCTGAAAA GATTCCCTCT GAGCTGCTGG GTCCCCTTGC TTGGGATAAC 
AACAATGCCA CCCAGATACC GAGAGAAGAG TGGAAATCCC AAAAGTCACA GAAAAACATA 
GCTTTTAAGA GCAAGGACAC CATTTTGCCC TTGGGCCCTT GTGAAAACAG TCCTTCAGTA 
GCAGCAATAA ATGAAGGGCA AGGTAAACTC CAAAGAGAAG CCACCAGGTC AAAGCAAGGA 
GAGACTGGAA GATTGTGCTC TCAGAACCCA CCGGTCTCAA AACGCCATCA AAGAGAAATA 
ACCCTTACTA CTCTTCAGTC AGAGGAAGAC AAAATTGAGT ATGATGATAC GTTTTCAATT 
GAAATGAAGA GAGAAGATTT TGACATCTAT GGTGAAGATG AAAATCAGGG CCTCCGCAGC 
TTTCAAAAGA GAACACGACA CTATTTCATT GCTGCAGTGG AGCGTCTCTG GGATTATGGG 
ATGAGTAGAT CTCCCCATGC ACTAAGAAAC AGGGCTCCAG GTGGGGATGT CCCTCAGTTC 
AAGAAGGTGG TTTTCCAGGA ATTTACTGAT GGATCCTTCA CTCAGCCCTT ATACCGTGGA 
GAGCTGAATG AACATTTGGG ACTCTTGGGG CCATATATAA GAGCAGAAGT TGAAGACAAT 
ATCATGGTAA CTTTCAAAAA CCAGGCTTCT CGTCCCTACT CCTTCTATTC TAGCCTGATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGAA AAAAGTTTGT CAACCCTAAT 
GAAACCAGAG TTTACTTCTG GAAAGTACAA CATCAGATGG CACCCACTGA AGATGAGTTT 
GACTGCAAGG CTTGGGCTTA TTTTTCTGAT GTTGATTTGG AAAAAGACAT GCACTCGGGC 
TTGATTGGAC CCCTTCTGAT CTGCCGCAGT AACACACTGA GCCCCGCTCA CGGGAGACAA 
GTGACAGTGC AGGAGTTTGC TCTGTTTTTC ACCATTTTTG ATGAGACTAA GAGCTGGTAC 
TTCACGGAAA ACATGGAAAG GAGCTGTAGA GCCCCCTGCA GTATCCAGAA GGAGGATCCC 
ACTTTTAAAG AAAAGTACCG CTTCCACGCA ATCAACGGCT ATGTGATGGA CACACTACCC 
GGCTTGGTAG TGGCTCAGGA TCAGAAGATC CGATGGTATC TGCTCAGCAT GGGGAGCAAT 
GAAAACATTC ATTCTATTCA TTTCAGTGGG CAGGTGTTCA CTGTACGGAA AAAAGAGGAA 
TATAAGATGG CAGTCTACAA CCTGTATCCA GGTGTTTTTG AGACAGTGGA AATGCTACCA 
TCCAAAGTTG GAATTTGGCG GATAGAATGC CTAATTGGCG AACACCTGCA AGCTGGGATG 
AGCACTCTCT TTCTGGTATA CAGCCCGAAG TGTCAGATTC CACTGGGAAT GGCTTCTGGA 
CGCATTAGAG ATTTCCAGAT CACAGCTTCA GGACAGTATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ACTATTCTGG ATCAATCAAT GCCTGGAGCA CCAAGGACCC CATTTCCTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATC ATTCACAGCA TCATGACCCA GGGCGCCCGT 
CAGAAGTTGT CCAGCCTGTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGAAAG 
AAGTGGCAGA GTTATCGAGG GAATTCCACT GGGACCTTAA TGGTGTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCGA TTATTGCTCG GTACATCCGT 
TTGCACCCAA CGCATTACAG CATCCGCAGC ACTCTCCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAACAGCT GCAGCATGCC ACTGGGAATG GAGAGTAAAG CAATATCAGA CGCACAGGTC 
ACCGCCTCGT CCTACTTAAA CAGCATGTTT GGCACTTGGT CCCCTTCACA AGCCCGGCTG 
CACCTCCAGG GGAGGACGAA TGCCTGGAGG CCTCAGGCAA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAG CCACCCAGGG GGTGAAATCA 
CTCCTTACCA GCATGTATGT GAAGGAGTTC CTCGTTTCCA GTAGTCAAGA TGGCCATAAC 
TGGGCTCTGT TTCTTCACAA TGGCAAAGTC AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCCGTGG TGAACTCTCT AGACCCCCCA CTACTGACTC GCTACCTTAG AATTCACCCC 
CAGAGCTGGG CACACCAGAT CGCCCTGAGG CTGGAGGTTC TGGGCTGTGA GGCCCAGCAG 
GTATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_019824516.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019680075.1 Felis catus coagulation factor VIII (F8), transcript variant X3, mRNA.

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CloneID	OFb58139
Clone ID Related Accession (Same CDS sequence)	XM_019824515.1 , XM_019824516.1 , XM_019824515.2
Accession Version	XM_019824516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	coagulation factor VIII isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGTG AACTGCTCAG TGAGCTGCAC GTGGACACAA GGTTATCCCC TGGGGTGCCA 
AGATCTTTGC CATTCAACAC ATCAGTCATG TACAGAAAGA CTGTGTTTGT AGAGTTTACA 
GATCGCCTTT TCAACATTGC CAAGCCCAGG CCACCATGGA TGGGTCTGCT GGGTCCTACC 
ATCCGGGCCG AAGTTTTTGA CACAGTGGTC ATTACACTCA AGAACATGGC TTCTCACCCT 
GTCAGTCTTC ATGCTGTTGG TGTATCCTAT TGGAAAGCTT CTGAAGGTGC TGAATATGAG 
GACCAGACCA GCCAGAAGGA GAAGGAAGAT GATAAAGTCT TTCCTGGTGA GAGTCATACC 
TACGTCTGGC AGGTCCTGAA AGAGAATGGA CCGATGGCCT CGGATCCTCC ATGTCTCACC 
TACTCATATT TTTCTCATGT GGACCTGGTG AAAGACCTGA ATTCAGGCCT CATTGGAGCC 
CTGCTGGTTT GCAAAGAAGG AAGTCTGGCT GAAGAAAGGA CACAGACCTT GCATGAATTT 
GTACTCCTGT TTGCTGTATT TGATGAAGGA AAAAGCTGGC ACTCATCTGC TCAGGCCCAG 
CATGAAATGC ACACGGTCAA CGGCTATGTC AACAGGTCTC TGCCAGGTCT GACTGGATGC 
CACAAGAAAT CAGTCTATTG GCATGTGATT GGAATGGGCA CCACCCCCGA AGTGCACTCA 
ATTTTTCTTG AAGGTCACAC ATTTCTCATC AGAAACCACC GCCAGGCCTC CTTGGAGATC 
TCACCAATAA CTTTCCTCAC TGCTCAGACA TTCCTGATGG ACCTTGGCCA GTTTCTACTG 
TTTTGTCACA TCTCTTCCCA CCAGCATGAT GGCATGGAAG CTTATGTCAA AGTAGATAGC 
TGCCCAGAGG AACCCCAGCT ACGGATGAAA ACTAATGAAA AAGAGGAAGA TTATGATGAT 
GATCTTTATG ATTCTGACAT GGACATGGTT AGGTTTGATG GCGACAGCCC TTCTCCCTTT 
ATTCAAATCC GCTCAGTTGC CAAGAAGCAT CCTAAAACTT GGATCCACTA TATTGCCGCC 
GAAGAGGAGG ACTGGGACTA TGCTCCCTCG GCCCCCACCC CCATTGACAG AAGTTATAAA 
CATCTATATT TGAACAGTGG TCCTCAGCGG ATTGGTAGGA AGTACAAAAA AGTTCGATTT 
ATGGCATACA CAGATGAAAC ATTTAAGACT CGTGAAGCTA TTCAGTATGA ATCAGGAATC 
CTAGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATATT TAAAAATCAA 
GCAAGCCGAC CATATAACAT CTACCCTCAT GGAATCACTG ACGTCAGTCC TTTGTACTCA 
GGGAGATTGC CTAAAGGTAT GAAACATTTG AAAGATATGC CAATTATGCC AGGAGAGATA 
TTCAAGTATA AATGGACAGT GACTGTAGAA GATGGGCCGA CTAAATCAGA TCCTCGGTGC 
CTGACTCGAT ATTACTCGAG CTTCATTAAT CCAGAGAGAG ATCTAGCTTC AGGACTCATT 
GGCCCTCTCC TCATATGCTA CAAAGGATCT GTAGATCAAA GAGGAAACCA GATGATGTCA 
GACAAGAGAA ACGTCATCCT GTTTTCTGTA TTTGATGAGA ACCAAAGCTG GTACCTTGCA 
GAGAATATGA AGCGCTTCCT CCCGAATGCA GATGCAGTGC CGCCCCACGA CCCGGAGCTC 
CAGGTCTCTA ACATCATGCA CAGCATCAAC GGCTATGTTT TTGACAACTT GCAGCTGTCA 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCACA AACTGACTTC 
CTGTCTGTCT TCTTCTCTGG ATACACCTTC AAACACAAAA TGGTCTATGA AGACACACTT 
ACCCTATTCC CATTCTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTATGG 
GTGCTGGGGT GCCACAACTC AGACTTTCGG AACAGAGGCA TGACAGCTTT ACTGAAGGTT 
TCTAGTTGTA ATGGGAACAT TGATGATTAT GATGAGGATA GATATGAAGA TATTCCAGCC 
TCCCTCCTGA ATGAAAACAA TGTCATTGAA CCCAGAAGCT TCTCCCAGAA TTCAAGACAT 
CATAGCCCTA GGCAAAAGCA ATTCAAAGCC ACCACAACTC CAGAAAATGA CATAGAGAAG 
ATTGACCCTC AGCCTGGAGA AAGAACGCAG CTGCTTAACG TACAAAGTGT CTCCTCTAGT 
GATTTGCTGA TGCTTCTGGG ACAGAATCCT ACTCCACGTG GATTACTCTT ATCTGATTTC 
CAGGAAGCCA CACATGAAGC AGATGATCAT TTACTTGGAG CTATAGAAAG ACACAAGGGC 
CCATCTGCAG TGGCAGATCT CAGACCAGAG CTCCATCACA GTGGGGACAG AGTATTTACT 
CCTGAGCCAA AACTAAAATT AAGATTAAAT GAGAATTTGG GGACAACTGT AACAGCAGAG 
TTGAAGAACC TTGATTTAAA AGTTTCTAGT TCATCAGACA ACCTAACAGC TTCACCAACA 
ATTCCATCAG ACAAGTTGGC AGCAGGTACT GAAAAGACAG GTTCCTTAGG ACCCCCAAAT 
ATGTCAATTC ACTTAAATAG TCATTTAGGT ACTATTGTAT TTGGCAAAAA TTCATCTCAC 
TCTATTGACT CTGGTGTACC TTTGGGCTCG AGTGAAGGAG ACAATGATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGTATT ATCAGTGGAG 
AGAAATGGGT TATTTAAAGA AGACAGAGTT CATGGACCTG CTTCATTAAT TAAAGATAAT 
GCTTTATTCA AAGTTAATAT CTCTTTGATA AAGACAAACA ACGCAACAAT TAACTCAACA 
ACTAATAGCA AAAATCATGT TGATGTCCCA ACATTATTAA CTGAGAACAG TACATCAGTC 
TGGAAAGATA CTATATTAGA AAGTAATGCT GAGTTTCAAG AAATGACTTC TTTGCTTCAT 
AATGAAACAT TTATGGACAG AAATATTACA GCTTTGGAGC TAAAGCATGT GTCAAATAAA 
ACTACTTCAT CAAAAAATGT GGAAGTGGTC TACCAAAACA AAGAGGGCTC GGTGCCACTA 
GGTGCAGAAA ATCCAGGTCT CTCATTCTTC AAGACACTGT TCTCACCAGA TTCAGCAAAG 
TGGATAAAAT GGACCCATGG CGAGAACTCC CTAAGCTCTG GGCAAAGGCC CATTCCAACA 
CAATTAACAT CTTTAGGATC AGAAAAATCT GTGAAAGATC AGAAGTTTTT GTCAGAAAAG 
AAGGTGGTAG TAGGAGAAGA TGAATTTACA AAGGACACAG AACTCAAAGA GATGAGTTTT 
CCAAACAGCA AGAGCATGTT TTTTACTAAC TTGCCCAATG TCCAAGATAA TGGTCCTTAC 
AATCAAGAAG AAAAATCTCA GGAAGAGATA GAAAGAAAGG AGAAATTAAC CCAAGAAAAT 
GTGGTTTTGC CTCAGGAATA TACAGTAGCT GACACTAATA ATTCCCTGAA GAACCTTTTC 
TTACTAAGCA CTAAGCAAAA TGTGGAAGGT TTAGATGAGG GGCCATATAC TCCAATACTT 
CAAGACACCA GGTCATTAAA TGATTCAGCA CATAGAGCAG GGATTCACAT AGTCCATTTC 
TCAAAAATAA GGGAGGAAGA AAACTTGGAA GGTTTGGGAA ATCAAACAAA GGAAATGGCA 
GAGAGGTTTT CAAGCACTCC AAGGATGTCT CCTAATCCAA GTCAGCATAA TATTCTCACT 
CAGCGTGGGA AGCGGGCCTT GAAACAATCC AGACTCTCAC TAGAAGAAAC TAAGCTTGCA 
AGGGGGGTAA TTTTGAATGA CACCTCAACC CAGTCATCCA AAAACATGAA CTTTTTGATG 
CATGGAACCC TCACACAGAT AGAGGACAAT GAGAAAGAAA AAGGGGCTGC CACTCAGTCC 
CTCCAATCAG ATTGTTCTAT GGGGAATCAG GGCACCATTC AAATGAATGA CTCTGCATTA 
CCCATTGCAA AGGAATCAGC ATTTCCATCA ATCAGACATA CAGATCTGAC CAAGATCCCA 
TCCCAGGACA ACTCTTCTCA TCTTCCAGTA TCAGCCTGCA CTTATACCTT CAGGGAGAGT 
TCTGGGGTCC AAAAAAGCAC TCATTTCTCA CAAGGAGCCA AGAGAAATAA ACTTTCTGTA 
GCCTTCCTAA CCTCAGAAAT GATTGTAGGT CAAGGAAAGT TCAGCTCCCT AGGGAAAAGT 
GCCATGAACC AACGCATGTC CCAGAAACGT GAAAACACTC TTTTGCAACA AGGCTTGTCT 
GAAGCATCTG GCAAAGTTGA ATTACTCCCT AAAGCTCATG TTCATCAAGA AGACTCTTTC 
CCTACAAAAA CTAGCAATGA TTCTTCTGGC CACCTGGATC TTATGAAGAA GATCTTCCTT 
CGGAAAACAC AGGGACCTAA GAAAATAAAT GGACCCGGAA AAGTGCCCTT CCTGAAATGG 
ACAACAGAGA GCTCTGAAAA GATTCCCTCT GAGCTGCTGG GTCCCCTTGC TTGGGATAAC 
AACAATGCCA CCCAGATACC GAGAGAAGAG TGGAAATCCC AAAAGTCACA GAAAAACATA 
GCTTTTAAGA GCAAGGACAC CATTTTGCCC TTGGGCCCTT GTGAAAACAG TCCTTCAGTA 
GCAGCAATAA ATGAAGGGCA AGGTAAACTC CAAAGAGAAG CCACCAGGTC AAAGCAAGGA 
GAGACTGGAA GATTGTGCTC TCAGAACCCA CCGGTCTCAA AACGCCATCA AAGAGAAATA 
ACCCTTACTA CTCTTCAGTC AGAGGAAGAC AAAATTGAGT ATGATGATAC GTTTTCAATT 
GAAATGAAGA GAGAAGATTT TGACATCTAT GGTGAAGATG AAAATCAGGG CCTCCGCAGC 
TTTCAAAAGA GAACACGACA CTATTTCATT GCTGCAGTGG AGCGTCTCTG GGATTATGGG 
ATGAGTAGAT CTCCCCATGC ACTAAGAAAC AGGGCTCCAG GTGGGGATGT CCCTCAGTTC 
AAGAAGGTGG TTTTCCAGGA ATTTACTGAT GGATCCTTCA CTCAGCCCTT ATACCGTGGA 
GAGCTGAATG AACATTTGGG ACTCTTGGGG CCATATATAA GAGCAGAAGT TGAAGACAAT 
ATCATGGTAA CTTTCAAAAA CCAGGCTTCT CGTCCCTACT CCTTCTATTC TAGCCTGATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGAA AAAAGTTTGT CAACCCTAAT 
GAAACCAGAG TTTACTTCTG GAAAGTACAA CATCAGATGG CACCCACTGA AGATGAGTTT 
GACTGCAAGG CTTGGGCTTA TTTTTCTGAT GTTGATTTGG AAAAAGACAT GCACTCGGGC 
TTGATTGGAC CCCTTCTGAT CTGCCGCAGT AACACACTGA GCCCCGCTCA CGGGAGACAA 
GTGACAGTGC AGGAGTTTGC TCTGTTTTTC ACCATTTTTG ATGAGACTAA GAGCTGGTAC 
TTCACGGAAA ACATGGAAAG GAGCTGTAGA GCCCCCTGCA GTATCCAGAA GGAGGATCCC 
ACTTTTAAAG AAAAGTACCG CTTCCACGCA ATCAACGGCT ATGTGATGGA CACACTACCC 
GGCTTGGTAG TGGCTCAGGA TCAGAAGATC CGATGGTATC TGCTCAGCAT GGGGAGCAAT 
GAAAACATTC ATTCTATTCA TTTCAGTGGG CAGGTGTTCA CTGTACGGAA AAAAGAGGAA 
TATAAGATGG CAGTCTACAA CCTGTATCCA GGTGTTTTTG AGACAGTGGA AATGCTACCA 
TCCAAAGTTG GAATTTGGCG GATAGAATGC CTAATTGGCG AACACCTGCA AGCTGGGATG 
AGCACTCTCT TTCTGGTATA CAGCCCGAAG TGTCAGATTC CACTGGGAAT GGCTTCTGGA 
CGCATTAGAG ATTTCCAGAT CACAGCTTCA GGACAGTATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ACTATTCTGG ATCAATCAAT GCCTGGAGCA CCAAGGACCC CATTTCCTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATC ATTCACAGCA TCATGACCCA GGGCGCCCGT 
CAGAAGTTGT CCAGCCTGTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGAAAG 
AAGTGGCAGA GTTATCGAGG GAATTCCACT GGGACCTTAA TGGTGTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCGA TTATTGCTCG GTACATCCGT 
TTGCACCCAA CGCATTACAG CATCCGCAGC ACTCTCCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAACAGCT GCAGCATGCC ACTGGGAATG GAGAGTAAAG CAATATCAGA CGCACAGGTC 
ACCGCCTCGT CCTACTTAAA CAGCATGTTT GGCACTTGGT CCCCTTCACA AGCCCGGCTG 
CACCTCCAGG GGAGGACGAA TGCCTGGAGG CCTCAGGCAA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAG CCACCCAGGG GGTGAAATCA 
CTCCTTACCA GCATGTATGT GAAGGAGTTC CTCGTTTCCA GTAGTCAAGA TGGCCATAAC 
TGGGCTCTGT TTCTTCACAA TGGCAAAGTC AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCCGTGG TGAACTCTCT AGACCCCCCA CTACTGACTC GCTACCTTAG AATTCACCCC 
CAGAGCTGGG CACACCAGAT CGCCCTGAGG CTGGAGGTTC TGGGCTGTGA GGCCCAGCAG 
GTATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019680075.1
CDS	96..7004
Translation	MQSELLSELHVDTRLSPGVPRSLPFNTSVMYRKTVFVEFTDRLFNIAKPRPPWMGLLGPTIRAEVFDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAEERTQTLHEFVLLFAVFDEGKSWHSSAQAQHEMHTVNGYVNRSLPGLTGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLIRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKTNEKEEDYDDDLYDSDMDMVRFDGDSPSPFIQIRSVAKKHPKTWIHYIAAEEEDWDYAPSAPTPIDRSYKHLYLNSGPQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLYSGRLPKGMKHLKDMPIMPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGLIGPLLICYKGSVDQRGNQMMSDKRNVILFSVFDENQSWYLAENMKRFLPNADAVPPHDPELQVSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNGNIDDYDEDRYEDIPASLLNENNVIEPRSFSQNSRHHSPRQKQFKATTTPENDIEKIDPQPGERTQLLNVQSVSSSDLLMLLGQNPTPRGLLLSDFQEATHEADDHLLGAIERHKGPSAVADLRPELHHSGDRVFTPEPKLKLRLNENLGTTVTAELKNLDLKVSSSSDNLTASPTIPSDKLAAGTEKTGSLGPPNMSIHLNSHLGTIVFGKNSSHSIDSGVPLGSSEGDNDSKLLEAALMNSQESSLGENVLSVERNGLFKEDRVHGPASLIKDNALFKVNISLIKTNNATINSTTNSKNHVDVPTLLTENSTSVWKDTILESNAEFQEMTSLLHNETFMDRNITALELKHVSNKTTSSKNVEVVYQNKEGSVPLGAENPGLSFFKTLFSPDSAKWIKWTHGENSLSSGQRPIPTQLTSLGSEKSVKDQKFLSEKKVVVGEDEFTKDTELKEMSFPNSKSMFFTNLPNVQDNGPYNQEEKSQEEIERKEKLTQENVVLPQEYTVADTNNSLKNLFLLSTKQNVEGLDEGPYTPILQDTRSLNDSAHRAGIHIVHFSKIREEENLEGLGNQTKEMAERFSSTPRMSPNPSQHNILTQRGKRALKQSRLSLEETKLARGVILNDTSTQSSKNMNFLMHGTLTQIEDNEKEKGAATQSLQSDCSMGNQGTIQMNDSALPIAKESAFPSIRHTDLTKIPSQDNSSHLPVSACTYTFRESSGVQKSTHFSQGAKRNKLSVAFLTSEMIVGQGKFSSLGKSAMNQRMSQKRENTLLQQGLSEASGKVELLPKAHVHQEDSFPTKTSNDSSGHLDLMKKIFLRKTQGPKKINGPGKVPFLKWTTESSEKIPSELLGPLAWDNNNATQIPREEWKSQKSQKNIAFKSKDTILPLGPCENSPSVAAINEGQGKLQREATRSKQGETGRLCSQNPPVSKRHQREITLTTLQSEEDKIEYDDTFSIEMKREDFDIYGEDENQGLRSFQKRTRHYFIAAVERLWDYGMSRSPHALRNRAPGGDVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKKFVNPNETRVYFWKVQHQMAPTEDEFDCKAWAYFSDVDLEKDMHSGLIGPLLICRSNTLSPAHGRQVTVQEFALFFTIFDETKSWYFTENMERSCRAPCSIQKEDPTFKEKYRFHAINGYVMDTLPGLVVAQDQKIRWYLLSMGSNENIHSIHFSGQVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKVGIWRIECLIGEHLQAGMSTLFLVYSPKCQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPISWIKVDLLAPMIIHSIMTQGARQKLSSLYISQFIIMYSLDGKKWQSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQVTASSYLNSMFGTWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFQKTMKVTGIATQGVKSLLTSMYVKEFLVSSSQDGHNWALFLHNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWAHQIALRLEVLGCEAQQVY

Target ORF information:

RefSeq Version	XM_019824516.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus coagulation factor VIII (F8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019824516.1

ORF Insert Sequence:

ATGCAAAGTG AACTGCTCAG TGAGCTGCAC GTGGACACAA GGTTATCCCC TGGGGTGCCA 
AGATCTTTGC CATTCAACAC ATCAGTCATG TACAGAAAGA CTGTGTTTGT AGAGTTTACA 
GATCGCCTTT TCAACATTGC CAAGCCCAGG CCACCATGGA TGGGTCTGCT GGGTCCTACC 
ATCCGGGCCG AAGTTTTTGA CACAGTGGTC ATTACACTCA AGAACATGGC TTCTCACCCT 
GTCAGTCTTC ATGCTGTTGG TGTATCCTAT TGGAAAGCTT CTGAAGGTGC TGAATATGAG 
GACCAGACCA GCCAGAAGGA GAAGGAAGAT GATAAAGTCT TTCCTGGTGA GAGTCATACC 
TACGTCTGGC AGGTCCTGAA AGAGAATGGA CCGATGGCCT CGGATCCTCC ATGTCTCACC 
TACTCATATT TTTCTCATGT GGACCTGGTG AAAGACCTGA ATTCAGGCCT CATTGGAGCC 
CTGCTGGTTT GCAAAGAAGG AAGTCTGGCT GAAGAAAGGA CACAGACCTT GCATGAATTT 
GTACTCCTGT TTGCTGTATT TGATGAAGGA AAAAGCTGGC ACTCATCTGC TCAGGCCCAG 
CATGAAATGC ACACGGTCAA CGGCTATGTC AACAGGTCTC TGCCAGGTCT GACTGGATGC 
CACAAGAAAT CAGTCTATTG GCATGTGATT GGAATGGGCA CCACCCCCGA AGTGCACTCA 
ATTTTTCTTG AAGGTCACAC ATTTCTCATC AGAAACCACC GCCAGGCCTC CTTGGAGATC 
TCACCAATAA CTTTCCTCAC TGCTCAGACA TTCCTGATGG ACCTTGGCCA GTTTCTACTG 
TTTTGTCACA TCTCTTCCCA CCAGCATGAT GGCATGGAAG CTTATGTCAA AGTAGATAGC 
TGCCCAGAGG AACCCCAGCT ACGGATGAAA ACTAATGAAA AAGAGGAAGA TTATGATGAT 
GATCTTTATG ATTCTGACAT GGACATGGTT AGGTTTGATG GCGACAGCCC TTCTCCCTTT 
ATTCAAATCC GCTCAGTTGC CAAGAAGCAT CCTAAAACTT GGATCCACTA TATTGCCGCC 
GAAGAGGAGG ACTGGGACTA TGCTCCCTCG GCCCCCACCC CCATTGACAG AAGTTATAAA 
CATCTATATT TGAACAGTGG TCCTCAGCGG ATTGGTAGGA AGTACAAAAA AGTTCGATTT 
ATGGCATACA CAGATGAAAC ATTTAAGACT CGTGAAGCTA TTCAGTATGA ATCAGGAATC 
CTAGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATATT TAAAAATCAA 
GCAAGCCGAC CATATAACAT CTACCCTCAT GGAATCACTG ACGTCAGTCC TTTGTACTCA 
GGGAGATTGC CTAAAGGTAT GAAACATTTG AAAGATATGC CAATTATGCC AGGAGAGATA 
TTCAAGTATA AATGGACAGT GACTGTAGAA GATGGGCCGA CTAAATCAGA TCCTCGGTGC 
CTGACTCGAT ATTACTCGAG CTTCATTAAT CCAGAGAGAG ATCTAGCTTC AGGACTCATT 
GGCCCTCTCC TCATATGCTA CAAAGGATCT GTAGATCAAA GAGGAAACCA GATGATGTCA 
GACAAGAGAA ACGTCATCCT GTTTTCTGTA TTTGATGAGA ACCAAAGCTG GTACCTTGCA 
GAGAATATGA AGCGCTTCCT CCCGAATGCA GATGCAGTGC CGCCCCACGA CCCGGAGCTC 
CAGGTCTCTA ACATCATGCA CAGCATCAAC GGCTATGTTT TTGACAACTT GCAGCTGTCA 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCACA AACTGACTTC 
CTGTCTGTCT TCTTCTCTGG ATACACCTTC AAACACAAAA TGGTCTATGA AGACACACTT 
ACCCTATTCC CATTCTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTATGG 
GTGCTGGGGT GCCACAACTC AGACTTTCGG AACAGAGGCA TGACAGCTTT ACTGAAGGTT 
TCTAGTTGTA ATGGGAACAT TGATGATTAT GATGAGGATA GATATGAAGA TATTCCAGCC 
TCCCTCCTGA ATGAAAACAA TGTCATTGAA CCCAGAAGCT TCTCCCAGAA TTCAAGACAT 
CATAGCCCTA GGCAAAAGCA ATTCAAAGCC ACCACAACTC CAGAAAATGA CATAGAGAAG 
ATTGACCCTC AGCCTGGAGA AAGAACGCAG CTGCTTAACG TACAAAGTGT CTCCTCTAGT 
GATTTGCTGA TGCTTCTGGG ACAGAATCCT ACTCCACGTG GATTACTCTT ATCTGATTTC 
CAGGAAGCCA CACATGAAGC AGATGATCAT TTACTTGGAG CTATAGAAAG ACACAAGGGC 
CCATCTGCAG TGGCAGATCT CAGACCAGAG CTCCATCACA GTGGGGACAG AGTATTTACT 
CCTGAGCCAA AACTAAAATT AAGATTAAAT GAGAATTTGG GGACAACTGT AACAGCAGAG 
TTGAAGAACC TTGATTTAAA AGTTTCTAGT TCATCAGACA ACCTAACAGC TTCACCAACA 
ATTCCATCAG ACAAGTTGGC AGCAGGTACT GAAAAGACAG GTTCCTTAGG ACCCCCAAAT 
ATGTCAATTC ACTTAAATAG TCATTTAGGT ACTATTGTAT TTGGCAAAAA TTCATCTCAC 
TCTATTGACT CTGGTGTACC TTTGGGCTCG AGTGAAGGAG ACAATGATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGTATT ATCAGTGGAG 
AGAAATGGGT TATTTAAAGA AGACAGAGTT CATGGACCTG CTTCATTAAT TAAAGATAAT 
GCTTTATTCA AAGTTAATAT CTCTTTGATA AAGACAAACA ACGCAACAAT TAACTCAACA 
ACTAATAGCA AAAATCATGT TGATGTCCCA ACATTATTAA CTGAGAACAG TACATCAGTC 
TGGAAAGATA CTATATTAGA AAGTAATGCT GAGTTTCAAG AAATGACTTC TTTGCTTCAT 
AATGAAACAT TTATGGACAG AAATATTACA GCTTTGGAGC TAAAGCATGT GTCAAATAAA 
ACTACTTCAT CAAAAAATGT GGAAGTGGTC TACCAAAACA AAGAGGGCTC GGTGCCACTA 
GGTGCAGAAA ATCCAGGTCT CTCATTCTTC AAGACACTGT TCTCACCAGA TTCAGCAAAG 
TGGATAAAAT GGACCCATGG CGAGAACTCC CTAAGCTCTG GGCAAAGGCC CATTCCAACA 
CAATTAACAT CTTTAGGATC AGAAAAATCT GTGAAAGATC AGAAGTTTTT GTCAGAAAAG 
AAGGTGGTAG TAGGAGAAGA TGAATTTACA AAGGACACAG AACTCAAAGA GATGAGTTTT 
CCAAACAGCA AGAGCATGTT TTTTACTAAC TTGCCCAATG TCCAAGATAA TGGTCCTTAC 
AATCAAGAAG AAAAATCTCA GGAAGAGATA GAAAGAAAGG AGAAATTAAC CCAAGAAAAT 
GTGGTTTTGC CTCAGGAATA TACAGTAGCT GACACTAATA ATTCCCTGAA GAACCTTTTC 
TTACTAAGCA CTAAGCAAAA TGTGGAAGGT TTAGATGAGG GGCCATATAC TCCAATACTT 
CAAGACACCA GGTCATTAAA TGATTCAGCA CATAGAGCAG GGATTCACAT AGTCCATTTC 
TCAAAAATAA GGGAGGAAGA AAACTTGGAA GGTTTGGGAA ATCAAACAAA GGAAATGGCA 
GAGAGGTTTT CAAGCACTCC AAGGATGTCT CCTAATCCAA GTCAGCATAA TATTCTCACT 
CAGCGTGGGA AGCGGGCCTT GAAACAATCC AGACTCTCAC TAGAAGAAAC TAAGCTTGCA 
AGGGGGGTAA TTTTGAATGA CACCTCAACC CAGTCATCCA AAAACATGAA CTTTTTGATG 
CATGGAACCC TCACACAGAT AGAGGACAAT GAGAAAGAAA AAGGGGCTGC CACTCAGTCC 
CTCCAATCAG ATTGTTCTAT GGGGAATCAG GGCACCATTC AAATGAATGA CTCTGCATTA 
CCCATTGCAA AGGAATCAGC ATTTCCATCA ATCAGACATA CAGATCTGAC CAAGATCCCA 
TCCCAGGACA ACTCTTCTCA TCTTCCAGTA TCAGCCTGCA CTTATACCTT CAGGGAGAGT 
TCTGGGGTCC AAAAAAGCAC TCATTTCTCA CAAGGAGCCA AGAGAAATAA ACTTTCTGTA 
GCCTTCCTAA CCTCAGAAAT GATTGTAGGT CAAGGAAAGT TCAGCTCCCT AGGGAAAAGT 
GCCATGAACC AACGCATGTC CCAGAAACGT GAAAACACTC TTTTGCAACA AGGCTTGTCT 
GAAGCATCTG GCAAAGTTGA ATTACTCCCT AAAGCTCATG TTCATCAAGA AGACTCTTTC 
CCTACAAAAA CTAGCAATGA TTCTTCTGGC CACCTGGATC TTATGAAGAA GATCTTCCTT 
CGGAAAACAC AGGGACCTAA GAAAATAAAT GGACCCGGAA AAGTGCCCTT CCTGAAATGG 
ACAACAGAGA GCTCTGAAAA GATTCCCTCT GAGCTGCTGG GTCCCCTTGC TTGGGATAAC 
AACAATGCCA CCCAGATACC GAGAGAAGAG TGGAAATCCC AAAAGTCACA GAAAAACATA 
GCTTTTAAGA GCAAGGACAC CATTTTGCCC TTGGGCCCTT GTGAAAACAG TCCTTCAGTA 
GCAGCAATAA ATGAAGGGCA AGGTAAACTC CAAAGAGAAG CCACCAGGTC AAAGCAAGGA 
GAGACTGGAA GATTGTGCTC TCAGAACCCA CCGGTCTCAA AACGCCATCA AAGAGAAATA 
ACCCTTACTA CTCTTCAGTC AGAGGAAGAC AAAATTGAGT ATGATGATAC GTTTTCAATT 
GAAATGAAGA GAGAAGATTT TGACATCTAT GGTGAAGATG AAAATCAGGG CCTCCGCAGC 
TTTCAAAAGA GAACACGACA CTATTTCATT GCTGCAGTGG AGCGTCTCTG GGATTATGGG 
ATGAGTAGAT CTCCCCATGC ACTAAGAAAC AGGGCTCCAG GTGGGGATGT CCCTCAGTTC 
AAGAAGGTGG TTTTCCAGGA ATTTACTGAT GGATCCTTCA CTCAGCCCTT ATACCGTGGA 
GAGCTGAATG AACATTTGGG ACTCTTGGGG CCATATATAA GAGCAGAAGT TGAAGACAAT 
ATCATGGTAA CTTTCAAAAA CCAGGCTTCT CGTCCCTACT CCTTCTATTC TAGCCTGATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGAA AAAAGTTTGT CAACCCTAAT 
GAAACCAGAG TTTACTTCTG GAAAGTACAA CATCAGATGG CACCCACTGA AGATGAGTTT 
GACTGCAAGG CTTGGGCTTA TTTTTCTGAT GTTGATTTGG AAAAAGACAT GCACTCGGGC 
TTGATTGGAC CCCTTCTGAT CTGCCGCAGT AACACACTGA GCCCCGCTCA CGGGAGACAA 
GTGACAGTGC AGGAGTTTGC TCTGTTTTTC ACCATTTTTG ATGAGACTAA GAGCTGGTAC 
TTCACGGAAA ACATGGAAAG GAGCTGTAGA GCCCCCTGCA GTATCCAGAA GGAGGATCCC 
ACTTTTAAAG AAAAGTACCG CTTCCACGCA ATCAACGGCT ATGTGATGGA CACACTACCC 
GGCTTGGTAG TGGCTCAGGA TCAGAAGATC CGATGGTATC TGCTCAGCAT GGGGAGCAAT 
GAAAACATTC ATTCTATTCA TTTCAGTGGG CAGGTGTTCA CTGTACGGAA AAAAGAGGAA 
TATAAGATGG CAGTCTACAA CCTGTATCCA GGTGTTTTTG AGACAGTGGA AATGCTACCA 
TCCAAAGTTG GAATTTGGCG GATAGAATGC CTAATTGGCG AACACCTGCA AGCTGGGATG 
AGCACTCTCT TTCTGGTATA CAGCCCGAAG TGTCAGATTC CACTGGGAAT GGCTTCTGGA 
CGCATTAGAG ATTTCCAGAT CACAGCTTCA GGACAGTATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ACTATTCTGG ATCAATCAAT GCCTGGAGCA CCAAGGACCC CATTTCCTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATC ATTCACAGCA TCATGACCCA GGGCGCCCGT 
CAGAAGTTGT CCAGCCTGTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGAAAG 
AAGTGGCAGA GTTATCGAGG GAATTCCACT GGGACCTTAA TGGTGTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCGA TTATTGCTCG GTACATCCGT 
TTGCACCCAA CGCATTACAG CATCCGCAGC ACTCTCCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAACAGCT GCAGCATGCC ACTGGGAATG GAGAGTAAAG CAATATCAGA CGCACAGGTC 
ACCGCCTCGT CCTACTTAAA CAGCATGTTT GGCACTTGGT CCCCTTCACA AGCCCGGCTG 
CACCTCCAGG GGAGGACGAA TGCCTGGAGG CCTCAGGCAA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAG CCACCCAGGG GGTGAAATCA 
CTCCTTACCA GCATGTATGT GAAGGAGTTC CTCGTTTCCA GTAGTCAAGA TGGCCATAAC 
TGGGCTCTGT TTCTTCACAA TGGCAAAGTC AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCCGTGG TGAACTCTCT AGACCCCCCA CTACTGACTC GCTACCTTAG AATTCACCCC 
CAGAGCTGGG CACACCAGAT CGCCCTGAGG CTGGAGGTTC TGGGCTGTGA GGCCCAGCAG 
GTATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_019824515.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019680074.1 Felis catus coagulation factor VIII (F8), transcript variant X2, mRNA.

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CloneID	OFb58139
Clone ID Related Accession (Same CDS sequence)	XM_019824515.1 , XM_019824516.1 , XM_019824515.2
Accession Version	XM_019824515.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	coagulation factor VIII isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019824515.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGTG AACTGCTCAG TGAGCTGCAC GTGGACACAA GGTTATCCCC TGGGGTGCCA 
AGATCTTTGC CATTCAACAC ATCAGTCATG TACAGAAAGA CTGTGTTTGT AGAGTTTACA 
GATCGCCTTT TCAACATTGC CAAGCCCAGG CCACCATGGA TGGGTCTGCT GGGTCCTACC 
ATCCGGGCCG AAGTTTTTGA CACAGTGGTC ATTACACTCA AGAACATGGC TTCTCACCCT 
GTCAGTCTTC ATGCTGTTGG TGTATCCTAT TGGAAAGCTT CTGAAGGTGC TGAATATGAG 
GACCAGACCA GCCAGAAGGA GAAGGAAGAT GATAAAGTCT TTCCTGGTGA GAGTCATACC 
TACGTCTGGC AGGTCCTGAA AGAGAATGGA CCGATGGCCT CGGATCCTCC ATGTCTCACC 
TACTCATATT TTTCTCATGT GGACCTGGTG AAAGACCTGA ATTCAGGCCT CATTGGAGCC 
CTGCTGGTTT GCAAAGAAGG AAGTCTGGCT GAAGAAAGGA CACAGACCTT GCATGAATTT 
GTACTCCTGT TTGCTGTATT TGATGAAGGA AAAAGCTGGC ACTCATCTGC TCAGGCCCAG 
CATGAAATGC ACACGGTCAA CGGCTATGTC AACAGGTCTC TGCCAGGTCT GACTGGATGC 
CACAAGAAAT CAGTCTATTG GCATGTGATT GGAATGGGCA CCACCCCCGA AGTGCACTCA 
ATTTTTCTTG AAGGTCACAC ATTTCTCATC AGAAACCACC GCCAGGCCTC CTTGGAGATC 
TCACCAATAA CTTTCCTCAC TGCTCAGACA TTCCTGATGG ACCTTGGCCA GTTTCTACTG 
TTTTGTCACA TCTCTTCCCA CCAGCATGAT GGCATGGAAG CTTATGTCAA AGTAGATAGC 
TGCCCAGAGG AACCCCAGCT ACGGATGAAA ACTAATGAAA AAGAGGAAGA TTATGATGAT 
GATCTTTATG ATTCTGACAT GGACATGGTT AGGTTTGATG GCGACAGCCC TTCTCCCTTT 
ATTCAAATCC GCTCAGTTGC CAAGAAGCAT CCTAAAACTT GGATCCACTA TATTGCCGCC 
GAAGAGGAGG ACTGGGACTA TGCTCCCTCG GCCCCCACCC CCATTGACAG AAGTTATAAA 
CATCTATATT TGAACAGTGG TCCTCAGCGG ATTGGTAGGA AGTACAAAAA AGTTCGATTT 
ATGGCATACA CAGATGAAAC ATTTAAGACT CGTGAAGCTA TTCAGTATGA ATCAGGAATC 
CTAGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATATT TAAAAATCAA 
GCAAGCCGAC CATATAACAT CTACCCTCAT GGAATCACTG ACGTCAGTCC TTTGTACTCA 
GGGAGATTGC CTAAAGGTAT GAAACATTTG AAAGATATGC CAATTATGCC AGGAGAGATA 
TTCAAGTATA AATGGACAGT GACTGTAGAA GATGGGCCGA CTAAATCAGA TCCTCGGTGC 
CTGACTCGAT ATTACTCGAG CTTCATTAAT CCAGAGAGAG ATCTAGCTTC AGGACTCATT 
GGCCCTCTCC TCATATGCTA CAAAGGATCT GTAGATCAAA GAGGAAACCA GATGATGTCA 
GACAAGAGAA ACGTCATCCT GTTTTCTGTA TTTGATGAGA ACCAAAGCTG GTACCTTGCA 
GAGAATATGA AGCGCTTCCT CCCGAATGCA GATGCAGTGC CGCCCCACGA CCCGGAGCTC 
CAGGTCTCTA ACATCATGCA CAGCATCAAC GGCTATGTTT TTGACAACTT GCAGCTGTCA 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCACA AACTGACTTC 
CTGTCTGTCT TCTTCTCTGG ATACACCTTC AAACACAAAA TGGTCTATGA AGACACACTT 
ACCCTATTCC CATTCTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTATGG 
GTGCTGGGGT GCCACAACTC AGACTTTCGG AACAGAGGCA TGACAGCTTT ACTGAAGGTT 
TCTAGTTGTA ATGGGAACAT TGATGATTAT GATGAGGATA GATATGAAGA TATTCCAGCC 
TCCCTCCTGA ATGAAAACAA TGTCATTGAA CCCAGAAGCT TCTCCCAGAA TTCAAGACAT 
CATAGCCCTA GGCAAAAGCA ATTCAAAGCC ACCACAACTC CAGAAAATGA CATAGAGAAG 
ATTGACCCTC AGCCTGGAGA AAGAACGCAG CTGCTTAACG TACAAAGTGT CTCCTCTAGT 
GATTTGCTGA TGCTTCTGGG ACAGAATCCT ACTCCACGTG GATTACTCTT ATCTGATTTC 
CAGGAAGCCA CACATGAAGC AGATGATCAT TTACTTGGAG CTATAGAAAG ACACAAGGGC 
CCATCTGCAG TGGCAGATCT CAGACCAGAG CTCCATCACA GTGGGGACAG AGTATTTACT 
CCTGAGCCAA AACTAAAATT AAGATTAAAT GAGAATTTGG GGACAACTGT AACAGCAGAG 
TTGAAGAACC TTGATTTAAA AGTTTCTAGT TCATCAGACA ACCTAACAGC TTCACCAACA 
ATTCCATCAG ACAAGTTGGC AGCAGGTACT GAAAAGACAG GTTCCTTAGG ACCCCCAAAT 
ATGTCAATTC ACTTAAATAG TCATTTAGGT ACTATTGTAT TTGGCAAAAA TTCATCTCAC 
TCTATTGACT CTGGTGTACC TTTGGGCTCG AGTGAAGGAG ACAATGATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGTATT ATCAGTGGAG 
AGAAATGGGT TATTTAAAGA AGACAGAGTT CATGGACCTG CTTCATTAAT TAAAGATAAT 
GCTTTATTCA AAGTTAATAT CTCTTTGATA AAGACAAACA ACGCAACAAT TAACTCAACA 
ACTAATAGCA AAAATCATGT TGATGTCCCA ACATTATTAA CTGAGAACAG TACATCAGTC 
TGGAAAGATA CTATATTAGA AAGTAATGCT GAGTTTCAAG AAATGACTTC TTTGCTTCAT 
AATGAAACAT TTATGGACAG AAATATTACA GCTTTGGAGC TAAAGCATGT GTCAAATAAA 
ACTACTTCAT CAAAAAATGT GGAAGTGGTC TACCAAAACA AAGAGGGCTC GGTGCCACTA 
GGTGCAGAAA ATCCAGGTCT CTCATTCTTC AAGACACTGT TCTCACCAGA TTCAGCAAAG 
TGGATAAAAT GGACCCATGG CGAGAACTCC CTAAGCTCTG GGCAAAGGCC CATTCCAACA 
CAATTAACAT CTTTAGGATC AGAAAAATCT GTGAAAGATC AGAAGTTTTT GTCAGAAAAG 
AAGGTGGTAG TAGGAGAAGA TGAATTTACA AAGGACACAG AACTCAAAGA GATGAGTTTT 
CCAAACAGCA AGAGCATGTT TTTTACTAAC TTGCCCAATG TCCAAGATAA TGGTCCTTAC 
AATCAAGAAG AAAAATCTCA GGAAGAGATA GAAAGAAAGG AGAAATTAAC CCAAGAAAAT 
GTGGTTTTGC CTCAGGAATA TACAGTAGCT GACACTAATA ATTCCCTGAA GAACCTTTTC 
TTACTAAGCA CTAAGCAAAA TGTGGAAGGT TTAGATGAGG GGCCATATAC TCCAATACTT 
CAAGACACCA GGTCATTAAA TGATTCAGCA CATAGAGCAG GGATTCACAT AGTCCATTTC 
TCAAAAATAA GGGAGGAAGA AAACTTGGAA GGTTTGGGAA ATCAAACAAA GGAAATGGCA 
GAGAGGTTTT CAAGCACTCC AAGGATGTCT CCTAATCCAA GTCAGCATAA TATTCTCACT 
CAGCGTGGGA AGCGGGCCTT GAAACAATCC AGACTCTCAC TAGAAGAAAC TAAGCTTGCA 
AGGGGGGTAA TTTTGAATGA CACCTCAACC CAGTCATCCA AAAACATGAA CTTTTTGATG 
CATGGAACCC TCACACAGAT AGAGGACAAT GAGAAAGAAA AAGGGGCTGC CACTCAGTCC 
CTCCAATCAG ATTGTTCTAT GGGGAATCAG GGCACCATTC AAATGAATGA CTCTGCATTA 
CCCATTGCAA AGGAATCAGC ATTTCCATCA ATCAGACATA CAGATCTGAC CAAGATCCCA 
TCCCAGGACA ACTCTTCTCA TCTTCCAGTA TCAGCCTGCA CTTATACCTT CAGGGAGAGT 
TCTGGGGTCC AAAAAAGCAC TCATTTCTCA CAAGGAGCCA AGAGAAATAA ACTTTCTGTA 
GCCTTCCTAA CCTCAGAAAT GATTGTAGGT CAAGGAAAGT TCAGCTCCCT AGGGAAAAGT 
GCCATGAACC AACGCATGTC CCAGAAACGT GAAAACACTC TTTTGCAACA AGGCTTGTCT 
GAAGCATCTG GCAAAGTTGA ATTACTCCCT AAAGCTCATG TTCATCAAGA AGACTCTTTC 
CCTACAAAAA CTAGCAATGA TTCTTCTGGC CACCTGGATC TTATGAAGAA GATCTTCCTT 
CGGAAAACAC AGGGACCTAA GAAAATAAAT GGACCCGGAA AAGTGCCCTT CCTGAAATGG 
ACAACAGAGA GCTCTGAAAA GATTCCCTCT GAGCTGCTGG GTCCCCTTGC TTGGGATAAC 
AACAATGCCA CCCAGATACC GAGAGAAGAG TGGAAATCCC AAAAGTCACA GAAAAACATA 
GCTTTTAAGA GCAAGGACAC CATTTTGCCC TTGGGCCCTT GTGAAAACAG TCCTTCAGTA 
GCAGCAATAA ATGAAGGGCA AGGTAAACTC CAAAGAGAAG CCACCAGGTC AAAGCAAGGA 
GAGACTGGAA GATTGTGCTC TCAGAACCCA CCGGTCTCAA AACGCCATCA AAGAGAAATA 
ACCCTTACTA CTCTTCAGTC AGAGGAAGAC AAAATTGAGT ATGATGATAC GTTTTCAATT 
GAAATGAAGA GAGAAGATTT TGACATCTAT GGTGAAGATG AAAATCAGGG CCTCCGCAGC 
TTTCAAAAGA GAACACGACA CTATTTCATT GCTGCAGTGG AGCGTCTCTG GGATTATGGG 
ATGAGTAGAT CTCCCCATGC ACTAAGAAAC AGGGCTCCAG GTGGGGATGT CCCTCAGTTC 
AAGAAGGTGG TTTTCCAGGA ATTTACTGAT GGATCCTTCA CTCAGCCCTT ATACCGTGGA 
GAGCTGAATG AACATTTGGG ACTCTTGGGG CCATATATAA GAGCAGAAGT TGAAGACAAT 
ATCATGGTAA CTTTCAAAAA CCAGGCTTCT CGTCCCTACT CCTTCTATTC TAGCCTGATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGAA AAAAGTTTGT CAACCCTAAT 
GAAACCAGAG TTTACTTCTG GAAAGTACAA CATCAGATGG CACCCACTGA AGATGAGTTT 
GACTGCAAGG CTTGGGCTTA TTTTTCTGAT GTTGATTTGG AAAAAGACAT GCACTCGGGC 
TTGATTGGAC CCCTTCTGAT CTGCCGCAGT AACACACTGA GCCCCGCTCA CGGGAGACAA 
GTGACAGTGC AGGAGTTTGC TCTGTTTTTC ACCATTTTTG ATGAGACTAA GAGCTGGTAC 
TTCACGGAAA ACATGGAAAG GAGCTGTAGA GCCCCCTGCA GTATCCAGAA GGAGGATCCC 
ACTTTTAAAG AAAAGTACCG CTTCCACGCA ATCAACGGCT ATGTGATGGA CACACTACCC 
GGCTTGGTAG TGGCTCAGGA TCAGAAGATC CGATGGTATC TGCTCAGCAT GGGGAGCAAT 
GAAAACATTC ATTCTATTCA TTTCAGTGGG CAGGTGTTCA CTGTACGGAA AAAAGAGGAA 
TATAAGATGG CAGTCTACAA CCTGTATCCA GGTGTTTTTG AGACAGTGGA AATGCTACCA 
TCCAAAGTTG GAATTTGGCG GATAGAATGC CTAATTGGCG AACACCTGCA AGCTGGGATG 
AGCACTCTCT TTCTGGTATA CAGCCCGAAG TGTCAGATTC CACTGGGAAT GGCTTCTGGA 
CGCATTAGAG ATTTCCAGAT CACAGCTTCA GGACAGTATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ACTATTCTGG ATCAATCAAT GCCTGGAGCA CCAAGGACCC CATTTCCTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATC ATTCACAGCA TCATGACCCA GGGCGCCCGT 
CAGAAGTTGT CCAGCCTGTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGAAAG 
AAGTGGCAGA GTTATCGAGG GAATTCCACT GGGACCTTAA TGGTGTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCGA TTATTGCTCG GTACATCCGT 
TTGCACCCAA CGCATTACAG CATCCGCAGC ACTCTCCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAACAGCT GCAGCATGCC ACTGGGAATG GAGAGTAAAG CAATATCAGA CGCACAGGTC 
ACCGCCTCGT CCTACTTAAA CAGCATGTTT GGCACTTGGT CCCCTTCACA AGCCCGGCTG 
CACCTCCAGG GGAGGACGAA TGCCTGGAGG CCTCAGGCAA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAG CCACCCAGGG GGTGAAATCA 
CTCCTTACCA GCATGTATGT GAAGGAGTTC CTCGTTTCCA GTAGTCAAGA TGGCCATAAC 
TGGGCTCTGT TTCTTCACAA TGGCAAAGTC AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCCGTGG TGAACTCTCT AGACCCCCCA CTACTGACTC GCTACCTTAG AATTCACCCC 
CAGAGCTGGG CACACCAGAT CGCCCTGAGG CTGGAGGTTC TGGGCTGTGA GGCCCAGCAG 
GTATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019680074.1
CDS	624..7532
Translation	MQSELLSELHVDTRLSPGVPRSLPFNTSVMYRKTVFVEFTDRLFNIAKPRPPWMGLLGPTIRAEVFDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAEERTQTLHEFVLLFAVFDEGKSWHSSAQAQHEMHTVNGYVNRSLPGLTGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLIRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKTNEKEEDYDDDLYDSDMDMVRFDGDSPSPFIQIRSVAKKHPKTWIHYIAAEEEDWDYAPSAPTPIDRSYKHLYLNSGPQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLYSGRLPKGMKHLKDMPIMPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGLIGPLLICYKGSVDQRGNQMMSDKRNVILFSVFDENQSWYLAENMKRFLPNADAVPPHDPELQVSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNGNIDDYDEDRYEDIPASLLNENNVIEPRSFSQNSRHHSPRQKQFKATTTPENDIEKIDPQPGERTQLLNVQSVSSSDLLMLLGQNPTPRGLLLSDFQEATHEADDHLLGAIERHKGPSAVADLRPELHHSGDRVFTPEPKLKLRLNENLGTTVTAELKNLDLKVSSSSDNLTASPTIPSDKLAAGTEKTGSLGPPNMSIHLNSHLGTIVFGKNSSHSIDSGVPLGSSEGDNDSKLLEAALMNSQESSLGENVLSVERNGLFKEDRVHGPASLIKDNALFKVNISLIKTNNATINSTTNSKNHVDVPTLLTENSTSVWKDTILESNAEFQEMTSLLHNETFMDRNITALELKHVSNKTTSSKNVEVVYQNKEGSVPLGAENPGLSFFKTLFSPDSAKWIKWTHGENSLSSGQRPIPTQLTSLGSEKSVKDQKFLSEKKVVVGEDEFTKDTELKEMSFPNSKSMFFTNLPNVQDNGPYNQEEKSQEEIERKEKLTQENVVLPQEYTVADTNNSLKNLFLLSTKQNVEGLDEGPYTPILQDTRSLNDSAHRAGIHIVHFSKIREEENLEGLGNQTKEMAERFSSTPRMSPNPSQHNILTQRGKRALKQSRLSLEETKLARGVILNDTSTQSSKNMNFLMHGTLTQIEDNEKEKGAATQSLQSDCSMGNQGTIQMNDSALPIAKESAFPSIRHTDLTKIPSQDNSSHLPVSACTYTFRESSGVQKSTHFSQGAKRNKLSVAFLTSEMIVGQGKFSSLGKSAMNQRMSQKRENTLLQQGLSEASGKVELLPKAHVHQEDSFPTKTSNDSSGHLDLMKKIFLRKTQGPKKINGPGKVPFLKWTTESSEKIPSELLGPLAWDNNNATQIPREEWKSQKSQKNIAFKSKDTILPLGPCENSPSVAAINEGQGKLQREATRSKQGETGRLCSQNPPVSKRHQREITLTTLQSEEDKIEYDDTFSIEMKREDFDIYGEDENQGLRSFQKRTRHYFIAAVERLWDYGMSRSPHALRNRAPGGDVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKKFVNPNETRVYFWKVQHQMAPTEDEFDCKAWAYFSDVDLEKDMHSGLIGPLLICRSNTLSPAHGRQVTVQEFALFFTIFDETKSWYFTENMERSCRAPCSIQKEDPTFKEKYRFHAINGYVMDTLPGLVVAQDQKIRWYLLSMGSNENIHSIHFSGQVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKVGIWRIECLIGEHLQAGMSTLFLVYSPKCQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPISWIKVDLLAPMIIHSIMTQGARQKLSSLYISQFIIMYSLDGKKWQSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQVTASSYLNSMFGTWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFQKTMKVTGIATQGVKSLLTSMYVKEFLVSSSQDGHNWALFLHNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWAHQIALRLEVLGCEAQQVY

Target ORF information:

RefSeq Version	XM_019824515.2
Organism	Felis catus(domestic cat)
Definition	Felis catus coagulation factor VIII (F8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019824515.2

ORF Insert Sequence:

ATGCAAAGTG AACTGCTCAG TGAGCTGCAC GTGGACACAA GGTTATCCCC TGGGGTGCCA 
AGATCTTTGC CATTCAACAC ATCAGTCATG TACAGAAAGA CTGTGTTTGT AGAGTTTACA 
GATCGCCTTT TCAACATTGC CAAGCCCAGG CCACCATGGA TGGGTCTGCT GGGTCCTACC 
ATCCGGGCCG AAGTTTTTGA CACAGTGGTC ATTACACTCA AGAACATGGC TTCTCACCCT 
GTCAGTCTTC ATGCTGTTGG TGTATCCTAT TGGAAAGCTT CTGAAGGTGC TGAATATGAG 
GACCAGACCA GCCAGAAGGA GAAGGAAGAT GATAAAGTCT TTCCTGGTGA GAGTCATACC 
TACGTCTGGC AGGTCCTGAA AGAGAATGGA CCGATGGCCT CGGATCCTCC ATGTCTCACC 
TACTCATATT TTTCTCATGT GGACCTGGTG AAAGACCTGA ATTCAGGCCT CATTGGAGCC 
CTGCTGGTTT GCAAAGAAGG AAGTCTGGCT GAAGAAAGGA CACAGACCTT GCATGAATTT 
GTACTCCTGT TTGCTGTATT TGATGAAGGA AAAAGCTGGC ACTCATCTGC TCAGGCCCAG 
CATGAAATGC ACACGGTCAA CGGCTATGTC AACAGGTCTC TGCCAGGTCT GACTGGATGC 
CACAAGAAAT CAGTCTATTG GCATGTGATT GGAATGGGCA CCACCCCCGA AGTGCACTCA 
ATTTTTCTTG AAGGTCACAC ATTTCTCATC AGAAACCACC GCCAGGCCTC CTTGGAGATC 
TCACCAATAA CTTTCCTCAC TGCTCAGACA TTCCTGATGG ACCTTGGCCA GTTTCTACTG 
TTTTGTCACA TCTCTTCCCA CCAGCATGAT GGCATGGAAG CTTATGTCAA AGTAGATAGC 
TGCCCAGAGG AACCCCAGCT ACGGATGAAA ACTAATGAAA AAGAGGAAGA TTATGATGAT 
GATCTTTATG ATTCTGACAT GGACATGGTT AGGTTTGATG GCGACAGCCC TTCTCCCTTT 
ATTCAAATCC GCTCAGTTGC CAAGAAGCAT CCTAAAACTT GGATCCACTA TATTGCCGCC 
GAAGAGGAGG ACTGGGACTA TGCTCCCTCG GCCCCCACCC CCATTGACAG AAGTTATAAA 
CATCTATATT TGAACAGTGG TCCTCAGCGG ATTGGTAGGA AGTACAAAAA AGTTCGATTT 
ATGGCATACA CAGATGAAAC ATTTAAGACT CGTGAAGCTA TTCAGTATGA ATCAGGAATC 
CTAGGACCTT TACTTTATGG AGAAGTTGGA GACACACTGT TGATTATATT TAAAAATCAA 
GCAAGCCGAC CATATAACAT CTACCCTCAT GGAATCACTG ACGTCAGTCC TTTGTACTCA 
GGGAGATTGC CTAAAGGTAT GAAACATTTG AAAGATATGC CAATTATGCC AGGAGAGATA 
TTCAAGTATA AATGGACAGT GACTGTAGAA GATGGGCCGA CTAAATCAGA TCCTCGGTGC 
CTGACTCGAT ATTACTCGAG CTTCATTAAT CCAGAGAGAG ATCTAGCTTC AGGACTCATT 
GGCCCTCTCC TCATATGCTA CAAAGGATCT GTAGATCAAA GAGGAAACCA GATGATGTCA 
GACAAGAGAA ACGTCATCCT GTTTTCTGTA TTTGATGAGA ACCAAAGCTG GTACCTTGCA 
GAGAATATGA AGCGCTTCCT CCCGAATGCA GATGCAGTGC CGCCCCACGA CCCGGAGCTC 
CAGGTCTCTA ACATCATGCA CAGCATCAAC GGCTATGTTT TTGACAACTT GCAGCTGTCA 
GTTTGTTTGC ATGAGGTGGC ATACTGGTAC ATTCTAAGTG TTGGAGCACA AACTGACTTC 
CTGTCTGTCT TCTTCTCTGG ATACACCTTC AAACACAAAA TGGTCTATGA AGACACACTT 
ACCCTATTCC CATTCTCAGG AGAAACTGTC TTCATGTCAA TGGAAAATCC AGGTCTATGG 
GTGCTGGGGT GCCACAACTC AGACTTTCGG AACAGAGGCA TGACAGCTTT ACTGAAGGTT 
TCTAGTTGTA ATGGGAACAT TGATGATTAT GATGAGGATA GATATGAAGA TATTCCAGCC 
TCCCTCCTGA ATGAAAACAA TGTCATTGAA CCCAGAAGCT TCTCCCAGAA TTCAAGACAT 
CATAGCCCTA GGCAAAAGCA ATTCAAAGCC ACCACAACTC CAGAAAATGA CATAGAGAAG 
ATTGACCCTC AGCCTGGAGA AAGAACGCAG CTGCTTAACG TACAAAGTGT CTCCTCTAGT 
GATTTGCTGA TGCTTCTGGG ACAGAATCCT ACTCCACGTG GATTACTCTT ATCTGATTTC 
CAGGAAGCCA CACATGAAGC AGATGATCAT TTACTTGGAG CTATAGAAAG ACACAAGGGC 
CCATCTGCAG TGGCAGATCT CAGACCAGAG CTCCATCACA GTGGGGACAG AGTATTTACT 
CCTGAGCCAA AACTAAAATT AAGATTAAAT GAGAATTTGG GGACAACTGT AACAGCAGAG 
TTGAAGAACC TTGATTTAAA AGTTTCTAGT TCATCAGACA ACCTAACAGC TTCACCAACA 
ATTCCATCAG ACAAGTTGGC AGCAGGTACT GAAAAGACAG GTTCCTTAGG ACCCCCAAAT 
ATGTCAATTC ACTTAAATAG TCATTTAGGT ACTATTGTAT TTGGCAAAAA TTCATCTCAC 
TCTATTGACT CTGGTGTACC TTTGGGCTCG AGTGAAGGAG ACAATGATTC CAAGTTGTTA 
GAAGCAGCTT TAATGAATAG TCAAGAAAGT TCACTGGGAG AAAATGTATT ATCAGTGGAG 
AGAAATGGGT TATTTAAAGA AGACAGAGTT CATGGACCTG CTTCATTAAT TAAAGATAAT 
GCTTTATTCA AAGTTAATAT CTCTTTGATA AAGACAAACA ACGCAACAAT TAACTCAACA 
ACTAATAGCA AAAATCATGT TGATGTCCCA ACATTATTAA CTGAGAACAG TACATCAGTC 
TGGAAAGATA CTATATTAGA AAGTAATGCT GAGTTTCAAG AAATGACTTC TTTGCTTCAT 
AATGAAACAT TTATGGACAG AAATATTACA GCTTTGGAGC TAAAGCATGT GTCAAATAAA 
ACTACTTCAT CAAAAAATGT GGAAGTGGTC TACCAAAACA AAGAGGGCTC GGTGCCACTA 
GGTGCAGAAA ATCCAGGTCT CTCATTCTTC AAGACACTGT TCTCACCAGA TTCAGCAAAG 
TGGATAAAAT GGACCCATGG CGAGAACTCC CTAAGCTCTG GGCAAAGGCC CATTCCAACA 
CAATTAACAT CTTTAGGATC AGAAAAATCT GTGAAAGATC AGAAGTTTTT GTCAGAAAAG 
AAGGTGGTAG TAGGAGAAGA TGAATTTACA AAGGACACAG AACTCAAAGA GATGAGTTTT 
CCAAACAGCA AGAGCATGTT TTTTACTAAC TTGCCCAATG TCCAAGATAA TGGTCCTTAC 
AATCAAGAAG AAAAATCTCA GGAAGAGATA GAAAGAAAGG AGAAATTAAC CCAAGAAAAT 
GTGGTTTTGC CTCAGGAATA TACAGTAGCT GACACTAATA ATTCCCTGAA GAACCTTTTC 
TTACTAAGCA CTAAGCAAAA TGTGGAAGGT TTAGATGAGG GGCCATATAC TCCAATACTT 
CAAGACACCA GGTCATTAAA TGATTCAGCA CATAGAGCAG GGATTCACAT AGTCCATTTC 
TCAAAAATAA GGGAGGAAGA AAACTTGGAA GGTTTGGGAA ATCAAACAAA GGAAATGGCA 
GAGAGGTTTT CAAGCACTCC AAGGATGTCT CCTAATCCAA GTCAGCATAA TATTCTCACT 
CAGCGTGGGA AGCGGGCCTT GAAACAATCC AGACTCTCAC TAGAAGAAAC TAAGCTTGCA 
AGGGGGGTAA TTTTGAATGA CACCTCAACC CAGTCATCCA AAAACATGAA CTTTTTGATG 
CATGGAACCC TCACACAGAT AGAGGACAAT GAGAAAGAAA AAGGGGCTGC CACTCAGTCC 
CTCCAATCAG ATTGTTCTAT GGGGAATCAG GGCACCATTC AAATGAATGA CTCTGCATTA 
CCCATTGCAA AGGAATCAGC ATTTCCATCA ATCAGACATA CAGATCTGAC CAAGATCCCA 
TCCCAGGACA ACTCTTCTCA TCTTCCAGTA TCAGCCTGCA CTTATACCTT CAGGGAGAGT 
TCTGGGGTCC AAAAAAGCAC TCATTTCTCA CAAGGAGCCA AGAGAAATAA ACTTTCTGTA 
GCCTTCCTAA CCTCAGAAAT GATTGTAGGT CAAGGAAAGT TCAGCTCCCT AGGGAAAAGT 
GCCATGAACC AACGCATGTC CCAGAAACGT GAAAACACTC TTTTGCAACA AGGCTTGTCT 
GAAGCATCTG GCAAAGTTGA ATTACTCCCT AAAGCTCATG TTCATCAAGA AGACTCTTTC 
CCTACAAAAA CTAGCAATGA TTCTTCTGGC CACCTGGATC TTATGAAGAA GATCTTCCTT 
CGGAAAACAC AGGGACCTAA GAAAATAAAT GGACCCGGAA AAGTGCCCTT CCTGAAATGG 
ACAACAGAGA GCTCTGAAAA GATTCCCTCT GAGCTGCTGG GTCCCCTTGC TTGGGATAAC 
AACAATGCCA CCCAGATACC GAGAGAAGAG TGGAAATCCC AAAAGTCACA GAAAAACATA 
GCTTTTAAGA GCAAGGACAC CATTTTGCCC TTGGGCCCTT GTGAAAACAG TCCTTCAGTA 
GCAGCAATAA ATGAAGGGCA AGGTAAACTC CAAAGAGAAG CCACCAGGTC AAAGCAAGGA 
GAGACTGGAA GATTGTGCTC TCAGAACCCA CCGGTCTCAA AACGCCATCA AAGAGAAATA 
ACCCTTACTA CTCTTCAGTC AGAGGAAGAC AAAATTGAGT ATGATGATAC GTTTTCAATT 
GAAATGAAGA GAGAAGATTT TGACATCTAT GGTGAAGATG AAAATCAGGG CCTCCGCAGC 
TTTCAAAAGA GAACACGACA CTATTTCATT GCTGCAGTGG AGCGTCTCTG GGATTATGGG 
ATGAGTAGAT CTCCCCATGC ACTAAGAAAC AGGGCTCCAG GTGGGGATGT CCCTCAGTTC 
AAGAAGGTGG TTTTCCAGGA ATTTACTGAT GGATCCTTCA CTCAGCCCTT ATACCGTGGA 
GAGCTGAATG AACATTTGGG ACTCTTGGGG CCATATATAA GAGCAGAAGT TGAAGACAAT 
ATCATGGTAA CTTTCAAAAA CCAGGCTTCT CGTCCCTACT CCTTCTATTC TAGCCTGATT 
TCTTATGAGG AAGATCAGAG GCAAGGAGCA GAACCTAGAA AAAAGTTTGT CAACCCTAAT 
GAAACCAGAG TTTACTTCTG GAAAGTACAA CATCAGATGG CACCCACTGA AGATGAGTTT 
GACTGCAAGG CTTGGGCTTA TTTTTCTGAT GTTGATTTGG AAAAAGACAT GCACTCGGGC 
TTGATTGGAC CCCTTCTGAT CTGCCGCAGT AACACACTGA GCCCCGCTCA CGGGAGACAA 
GTGACAGTGC AGGAGTTTGC TCTGTTTTTC ACCATTTTTG ATGAGACTAA GAGCTGGTAC 
TTCACGGAAA ACATGGAAAG GAGCTGTAGA GCCCCCTGCA GTATCCAGAA GGAGGATCCC 
ACTTTTAAAG AAAAGTACCG CTTCCACGCA ATCAACGGCT ATGTGATGGA CACACTACCC 
GGCTTGGTAG TGGCTCAGGA TCAGAAGATC CGATGGTATC TGCTCAGCAT GGGGAGCAAT 
GAAAACATTC ATTCTATTCA TTTCAGTGGG CAGGTGTTCA CTGTACGGAA AAAAGAGGAA 
TATAAGATGG CAGTCTACAA CCTGTATCCA GGTGTTTTTG AGACAGTGGA AATGCTACCA 
TCCAAAGTTG GAATTTGGCG GATAGAATGC CTAATTGGCG AACACCTGCA AGCTGGGATG 
AGCACTCTCT TTCTGGTATA CAGCCCGAAG TGTCAGATTC CACTGGGAAT GGCTTCTGGA 
CGCATTAGAG ATTTCCAGAT CACAGCTTCA GGACAGTATG GACAGTGGGC CCCAAAGCTG 
GCCAGACTTC ACTATTCTGG ATCAATCAAT GCCTGGAGCA CCAAGGACCC CATTTCCTGG 
ATCAAGGTGG ATCTGTTGGC ACCAATGATC ATTCACAGCA TCATGACCCA GGGCGCCCGT 
CAGAAGTTGT CCAGCCTGTA CATCTCTCAG TTTATCATCA TGTATAGTCT TGATGGAAAG 
AAGTGGCAGA GTTATCGAGG GAATTCCACT GGGACCTTAA TGGTGTTCTT TGGCAATGTG 
GATTCATCTG GGATAAAACA CAATATTTTT AACCCTCCGA TTATTGCTCG GTACATCCGT 
TTGCACCCAA CGCATTACAG CATCCGCAGC ACTCTCCGCA TGGAGTTGAT GGGCTGTGAT 
TTAAACAGCT GCAGCATGCC ACTGGGAATG GAGAGTAAAG CAATATCAGA CGCACAGGTC 
ACCGCCTCGT CCTACTTAAA CAGCATGTTT GGCACTTGGT CCCCTTCACA AGCCCGGCTG 
CACCTCCAGG GGAGGACGAA TGCCTGGAGG CCTCAGGCAA ATAATCCAAA AGAGTGGCTG 
CAAGTGGACT TCCAGAAGAC AATGAAAGTC ACAGGAATAG CCACCCAGGG GGTGAAATCA 
CTCCTTACCA GCATGTATGT GAAGGAGTTC CTCGTTTCCA GTAGTCAAGA TGGCCATAAC 
TGGGCTCTGT TTCTTCACAA TGGCAAAGTC AAGGTTTTTC AGGGAAATCA AGACTCCTTC 
ACACCCGTGG TGAACTCTCT AGACCCCCCA CTACTGACTC GCTACCTTAG AATTCACCCC 
CAGAGCTGGG CACACCAGAT CGCCCTGAGG CTGGAGGTTC TGGGCTGTGA GGCCCAGCAG 
GTATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_019824517.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019680076.1 Felis catus coagulation factor VIII (F8), transcript variant X4, mRNA.

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CloneID	OFb58140
Clone ID Related Accession (Same CDS sequence)	XM_019824517.1
Accession Version	XM_019824517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	coagulation factor VIII isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA AGACTGTGTT TGTAGAGTTT ACAGATCGCC TTTTCAACAT TGCCAAGCCC 
AGGCCACCAT GGATGGGTCT GCTGGGTCCT ACCATCCGGG CCGAAGTTTT TGACACAGTG 
GTCATTACAC TCAAGAACAT GGCTTCTCAC CCTGTCAGTC TTCATGCTGT TGGTGTATCC 
TATTGGAAAG CTTCTGAAGG TGCTGAATAT GAGGACCAGA CCAGCCAGAA GGAGAAGGAA 
GATGATAAAG TCTTTCCTGG TGAGAGTCAT ACCTACGTCT GGCAGGTCCT GAAAGAGAAT 
GGACCGATGG CCTCGGATCC TCCATGTCTC ACCTACTCAT ATTTTTCTCA TGTGGACCTG 
GTGAAAGACC TGAATTCAGG CCTCATTGGA GCCCTGCTGG TTTGCAAAGA AGGAAGTCTG 
GCTGAAGAAA GGACACAGAC CTTGCATGAA TTTGTACTCC TGTTTGCTGT ATTTGATGAA 
GGAAAAAGCT GGCACTCATC TGCTCAGGCC CAGCATGAAA TGCACACGGT CAACGGCTAT 
GTCAACAGGT CTCTGCCAGG TCTGACTGGA TGCCACAAGA AATCAGTCTA TTGGCATGTG 
ATTGGAATGG GCACCACCCC CGAAGTGCAC TCAATTTTTC TTGAAGGTCA CACATTTCTC 
ATCAGAAACC ACCGCCAGGC CTCCTTGGAG ATCTCACCAA TAACTTTCCT CACTGCTCAG 
ACATTCCTGA TGGACCTTGG CCAGTTTCTA CTGTTTTGTC ACATCTCTTC CCACCAGCAT 
GATGGCATGG AAGCTTATGT CAAAGTAGAT AGCTGCCCAG AGGAACCCCA GCTACGGATG 
AAAACTAATG AAAAAGAGGA AGATTATGAT GATGATCTTT ATGATTCTGA CATGGACATG 
GTTAGGTTTG ATGGCGACAG CCCTTCTCCC TTTATTCAAA TCCGCTCAGT TGCCAAGAAG 
CATCCTAAAA CTTGGATCCA CTATATTGCC GCCGAAGAGG AGGACTGGGA CTATGCTCCC 
TCGGCCCCCA CCCCCATTGA CAGAAGTTAT AAACATCTAT ATTTGAACAG TGGTCCTCAG 
CGGATTGGTA GGAAGTACAA AAAAGTTCGA TTTATGGCAT ACACAGATGA AACATTTAAG 
ACTCGTGAAG CTATTCAGTA TGAATCAGGA ATCCTAGGAC CTTTACTTTA TGGAGAAGTT 
GGAGACACAC TGTTGATTAT ATTTAAAAAT CAAGCAAGCC GACCATATAA CATCTACCCT 
CATGGAATCA CTGACGTCAG TCCTTTGTAC TCAGGGAGAT TGCCTAAAGG TATGAAACAT 
TTGAAAGATA TGCCAATTAT GCCAGGAGAG ATATTCAAGT ATAAATGGAC AGTGACTGTA 
GAAGATGGGC CGACTAAATC AGATCCTCGG TGCCTGACTC GATATTACTC GAGCTTCATT 
AATCCAGAGA GAGATCTAGC TTCAGGACTC ATTGGCCCTC TCCTCATATG CTACAAAGGA 
TCTGTAGATC AAAGAGGAAA CCAGATGATG TCAGACAAGA GAAACGTCAT CCTGTTTTCT 
GTATTTGATG AGAACCAAAG CTGGTACCTT GCAGAGAATA TGAAGCGCTT CCTCCCGAAT 
GCAGATGCAG TGCCGCCCCA CGACCCGGAG CTCCAGGTCT CTAACATCAT GCACAGCATC 
AACGGCTATG TTTTTGACAA CTTGCAGCTG TCAGTTTGTT TGCATGAGGT GGCATACTGG 
TACATTCTAA GTGTTGGAGC ACAAACTGAC TTCCTGTCTG TCTTCTTCTC TGGATACACC 
TTCAAACACA AAATGGTCTA TGAAGACACA CTTACCCTAT TCCCATTCTC AGGAGAAACT 
GTCTTCATGT CAATGGAAAA TCCAGGTCTA TGGGTGCTGG GGTGCCACAA CTCAGACTTT 
CGGAACAGAG GCATGACAGC TTTACTGAAG GTTTCTAGTT GTAATGGGAA CATTGATGAT 
TATGATGAGG ATAGATATGA AGATATTCCA GCCTCCCTCC TGAATGAAAA CAATGTCATT 
GAACCCAGAA GCTTCTCCCA GAATTCAAGA CATCATAGCC CTAGGCAAAA GCAATTCAAA 
GCCACCACAA CTCCAGAAAA TGACATAGAG AAGATTGACC CTCAGCCTGG AGAAAGAACG 
CAGCTGCTTA ACGTACAAAG TGTCTCCTCT AGTGATTTGC TGATGCTTCT GGGACAGAAT 
CCTACTCCAC GTGGATTACT CTTATCTGAT TTCCAGGAAG CCACACATGA AGCAGATGAT 
CATTTACTTG GAGCTATAGA AAGACACAAG GGCCCATCTG CAGTGGCAGA TCTCAGACCA 
GAGCTCCATC ACAGTGGGGA CAGAGTATTT ACTCCTGAGC CAAAACTAAA ATTAAGATTA 
AATGAGAATT TGGGGACAAC TGTAACAGCA GAGTTGAAGA ACCTTGATTT AAAAGTTTCT 
AGTTCATCAG ACAACCTAAC AGCTTCACCA ACAATTCCAT CAGACAAGTT GGCAGCAGGT 
ACTGAAAAGA CAGGTTCCTT AGGACCCCCA AATATGTCAA TTCACTTAAA TAGTCATTTA 
GGTACTATTG TATTTGGCAA AAATTCATCT CACTCTATTG ACTCTGGTGT ACCTTTGGGC 
TCGAGTGAAG GAGACAATGA TTCCAAGTTG TTAGAAGCAG CTTTAATGAA TAGTCAAGAA 
AGTTCACTGG GAGAAAATGT ATTATCAGTG GAGAGAAATG GGTTATTTAA AGAAGACAGA 
GTTCATGGAC CTGCTTCATT AATTAAAGAT AATGCTTTAT TCAAAGTTAA TATCTCTTTG 
ATAAAGACAA ACAACGCAAC AATTAACTCA ACAACTAATA GCAAAAATCA TGTTGATGTC 
CCAACATTAT TAACTGAGAA CAGTACATCA GTCTGGAAAG ATACTATATT AGAAAGTAAT 
GCTGAGTTTC AAGAAATGAC TTCTTTGCTT CATAATGAAA CATTTATGGA CAGAAATATT 
ACAGCTTTGG AGCTAAAGCA TGTGTCAAAT AAAACTACTT CATCAAAAAA TGTGGAAGTG 
GTCTACCAAA ACAAAGAGGG CTCGGTGCCA CTAGGTGCAG AAAATCCAGG TCTCTCATTC 
TTCAAGACAC TGTTCTCACC AGATTCAGCA AAGTGGATAA AATGGACCCA TGGCGAGAAC 
TCCCTAAGCT CTGGGCAAAG GCCCATTCCA ACACAATTAA CATCTTTAGG ATCAGAAAAA 
TCTGTGAAAG ATCAGAAGTT TTTGTCAGAA AAGAAGGTGG TAGTAGGAGA AGATGAATTT 
ACAAAGGACA CAGAACTCAA AGAGATGAGT TTTCCAAACA GCAAGAGCAT GTTTTTTACT 
AACTTGCCCA ATGTCCAAGA TAATGGTCCT TACAATCAAG AAGAAAAATC TCAGGAAGAG 
ATAGAAAGAA AGGAGAAATT AACCCAAGAA AATGTGGTTT TGCCTCAGGA ATATACAGTA 
GCTGACACTA ATAATTCCCT GAAGAACCTT TTCTTACTAA GCACTAAGCA AAATGTGGAA 
GGTTTAGATG AGGGGCCATA TACTCCAATA CTTCAAGACA CCAGGTCATT AAATGATTCA 
GCACATAGAG CAGGGATTCA CATAGTCCAT TTCTCAAAAA TAAGGGAGGA AGAAAACTTG 
GAAGGTTTGG GAAATCAAAC AAAGGAAATG GCAGAGAGGT TTTCAAGCAC TCCAAGGATG 
TCTCCTAATC CAAGTCAGCA TAATATTCTC ACTCAGCGTG GGAAGCGGGC CTTGAAACAA 
TCCAGACTCT CACTAGAAGA AACTAAGCTT GCAAGGGGGG TAATTTTGAA TGACACCTCA 
ACCCAGTCAT CCAAAAACAT GAACTTTTTG ATGCATGGAA CCCTCACACA GATAGAGGAC 
AATGAGAAAG AAAAAGGGGC TGCCACTCAG TCCCTCCAAT CAGATTGTTC TATGGGGAAT 
CAGGGCACCA TTCAAATGAA TGACTCTGCA TTACCCATTG CAAAGGAATC AGCATTTCCA 
TCAATCAGAC ATACAGATCT GACCAAGATC CCATCCCAGG ACAACTCTTC TCATCTTCCA 
GTATCAGCCT GCACTTATAC CTTCAGGGAG AGTTCTGGGG TCCAAAAAAG CACTCATTTC 
TCACAAGGAG CCAAGAGAAA TAAACTTTCT GTAGCCTTCC TAACCTCAGA AATGATTGTA 
GGTCAAGGAA AGTTCAGCTC CCTAGGGAAA AGTGCCATGA ACCAACGCAT GTCCCAGAAA 
CGTGAAAACA CTCTTTTGCA ACAAGGCTTG TCTGAAGCAT CTGGCAAAGT TGAATTACTC 
CCTAAAGCTC ATGTTCATCA AGAAGACTCT TTCCCTACAA AAACTAGCAA TGATTCTTCT 
GGCCACCTGG ATCTTATGAA GAAGATCTTC CTTCGGAAAA CACAGGGACC TAAGAAAATA 
AATGGACCCG GAAAAGTGCC CTTCCTGAAA TGGACAACAG AGAGCTCTGA AAAGATTCCC 
TCTGAGCTGC TGGGTCCCCT TGCTTGGGAT AACAACAATG CCACCCAGAT ACCGAGAGAA 
GAGTGGAAAT CCCAAAAGTC ACAGAAAAAC ATAGCTTTTA AGAGCAAGGA CACCATTTTG 
CCCTTGGGCC CTTGTGAAAA CAGTCCTTCA GTAGCAGCAA TAAATGAAGG GCAAGGTAAA 
CTCCAAAGAG AAGCCACCAG GTCAAAGCAA GGAGAGACTG GAAGATTGTG CTCTCAGAAC 
CCACCGGTCT CAAAACGCCA TCAAAGAGAA ATAACCCTTA CTACTCTTCA GTCAGAGGAA 
GACAAAATTG AGTATGATGA TACGTTTTCA ATTGAAATGA AGAGAGAAGA TTTTGACATC 
TATGGTGAAG ATGAAAATCA GGGCCTCCGC AGCTTTCAAA AGAGAACACG ACACTATTTC 
ATTGCTGCAG TGGAGCGTCT CTGGGATTAT GGGATGAGTA GATCTCCCCA TGCACTAAGA 
AACAGGGCTC CAGGTGGGGA TGTCCCTCAG TTCAAGAAGG TGGTTTTCCA GGAATTTACT 
GATGGATCCT TCACTCAGCC CTTATACCGT GGAGAGCTGA ATGAACATTT GGGACTCTTG 
GGGCCATATA TAAGAGCAGA AGTTGAAGAC AATATCATGG TAACTTTCAA AAACCAGGCT 
TCTCGTCCCT ACTCCTTCTA TTCTAGCCTG ATTTCTTATG AGGAAGATCA GAGGCAAGGA 
GCAGAACCTA GAAAAAAGTT TGTCAACCCT AATGAAACCA GAGTTTACTT CTGGAAAGTA 
CAACATCAGA TGGCACCCAC TGAAGATGAG TTTGACTGCA AGGCTTGGGC TTATTTTTCT 
GATGTTGATT TGGAAAAAGA CATGCACTCG GGCTTGATTG GACCCCTTCT GATCTGCCGC 
AGTAACACAC TGAGCCCCGC TCACGGGAGA CAAGTGACAG TGCAGGAGTT TGCTCTGTTT 
TTCACCATTT TTGATGAGAC TAAGAGCTGG TACTTCACGG AAAACATGGA AAGGAGCTGT 
AGAGCCCCCT GCAGTATCCA GAAGGAGGAT CCCACTTTTA AAGAAAAGTA CCGCTTCCAC 
GCAATCAACG GCTATGTGAT GGACACACTA CCCGGCTTGG TAGTGGCTCA GGATCAGAAG 
ATCCGATGGT ATCTGCTCAG CATGGGGAGC AATGAAAACA TTCATTCTAT TCATTTCAGT 
GGGCAGGTGT TCACTGTACG GAAAAAAGAG GAATATAAGA TGGCAGTCTA CAACCTGTAT 
CCAGGTGTTT TTGAGACAGT GGAAATGCTA CCATCCAAAG TTGGAATTTG GCGGATAGAA 
TGCCTAATTG GCGAACACCT GCAAGCTGGG ATGAGCACTC TCTTTCTGGT ATACAGCCCG 
AAGTGTCAGA TTCCACTGGG AATGGCTTCT GGACGCATTA GAGATTTCCA GATCACAGCT 
TCAGGACAGT ATGGACAGTG GGCCCCAAAG CTGGCCAGAC TTCACTATTC TGGATCAATC 
AATGCCTGGA GCACCAAGGA CCCCATTTCC TGGATCAAGG TGGATCTGTT GGCACCAATG 
ATCATTCACA GCATCATGAC CCAGGGCGCC CGTCAGAAGT TGTCCAGCCT GTACATCTCT 
CAGTTTATCA TCATGTATAG TCTTGATGGA AAGAAGTGGC AGAGTTATCG AGGGAATTCC 
ACTGGGACCT TAATGGTGTT CTTTGGCAAT GTGGATTCAT CTGGGATAAA ACACAATATT 
TTTAACCCTC CGATTATTGC TCGGTACATC CGTTTGCACC CAACGCATTA CAGCATCCGC 
AGCACTCTCC GCATGGAGTT GATGGGCTGT GATTTAAACA GCTGCAGCAT GCCACTGGGA 
ATGGAGAGTA AAGCAATATC AGACGCACAG GTCACCGCCT CGTCCTACTT AAACAGCATG 
TTTGGCACTT GGTCCCCTTC ACAAGCCCGG CTGCACCTCC AGGGGAGGAC GAATGCCTGG 
AGGCCTCAGG CAAATAATCC AAAAGAGTGG CTGCAAGTGG ACTTCCAGAA GACAATGAAA 
GTCACAGGAA TAGCCACCCA GGGGGTGAAA TCACTCCTTA CCAGCATGTA TGTGAAGGAG 
TTCCTCGTTT CCAGTAGTCA AGATGGCCAT AACTGGGCTC TGTTTCTTCA CAATGGCAAA 
GTCAAGGTTT TTCAGGGAAA TCAAGACTCC TTCACACCCG TGGTGAACTC TCTAGACCCC 
CCACTACTGA CTCGCTACCT TAGAATTCAC CCCCAGAGCT GGGCACACCA GATCGCCCTG 
AGGCTGGAGG TTCTGGGCTG TGAGGCCCAG CAGGTATACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019680076.1
CDS	601..7422
Translation	MYRKTVFVEFTDRLFNIAKPRPPWMGLLGPTIRAEVFDTVVITLKNMASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAEERTQTLHEFVLLFAVFDEGKSWHSSAQAQHEMHTVNGYVNRSLPGLTGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLIRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKTNEKEEDYDDDLYDSDMDMVRFDGDSPSPFIQIRSVAKKHPKTWIHYIAAEEEDWDYAPSAPTPIDRSYKHLYLNSGPQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLYSGRLPKGMKHLKDMPIMPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGLIGPLLICYKGSVDQRGNQMMSDKRNVILFSVFDENQSWYLAENMKRFLPNADAVPPHDPELQVSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNGNIDDYDEDRYEDIPASLLNENNVIEPRSFSQNSRHHSPRQKQFKATTTPENDIEKIDPQPGERTQLLNVQSVSSSDLLMLLGQNPTPRGLLLSDFQEATHEADDHLLGAIERHKGPSAVADLRPELHHSGDRVFTPEPKLKLRLNENLGTTVTAELKNLDLKVSSSSDNLTASPTIPSDKLAAGTEKTGSLGPPNMSIHLNSHLGTIVFGKNSSHSIDSGVPLGSSEGDNDSKLLEAALMNSQESSLGENVLSVERNGLFKEDRVHGPASLIKDNALFKVNISLIKTNNATINSTTNSKNHVDVPTLLTENSTSVWKDTILESNAEFQEMTSLLHNETFMDRNITALELKHVSNKTTSSKNVEVVYQNKEGSVPLGAENPGLSFFKTLFSPDSAKWIKWTHGENSLSSGQRPIPTQLTSLGSEKSVKDQKFLSEKKVVVGEDEFTKDTELKEMSFPNSKSMFFTNLPNVQDNGPYNQEEKSQEEIERKEKLTQENVVLPQEYTVADTNNSLKNLFLLSTKQNVEGLDEGPYTPILQDTRSLNDSAHRAGIHIVHFSKIREEENLEGLGNQTKEMAERFSSTPRMSPNPSQHNILTQRGKRALKQSRLSLEETKLARGVILNDTSTQSSKNMNFLMHGTLTQIEDNEKEKGAATQSLQSDCSMGNQGTIQMNDSALPIAKESAFPSIRHTDLTKIPSQDNSSHLPVSACTYTFRESSGVQKSTHFSQGAKRNKLSVAFLTSEMIVGQGKFSSLGKSAMNQRMSQKRENTLLQQGLSEASGKVELLPKAHVHQEDSFPTKTSNDSSGHLDLMKKIFLRKTQGPKKINGPGKVPFLKWTTESSEKIPSELLGPLAWDNNNATQIPREEWKSQKSQKNIAFKSKDTILPLGPCENSPSVAAINEGQGKLQREATRSKQGETGRLCSQNPPVSKRHQREITLTTLQSEEDKIEYDDTFSIEMKREDFDIYGEDENQGLRSFQKRTRHYFIAAVERLWDYGMSRSPHALRNRAPGGDVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKKFVNPNETRVYFWKVQHQMAPTEDEFDCKAWAYFSDVDLEKDMHSGLIGPLLICRSNTLSPAHGRQVTVQEFALFFTIFDETKSWYFTENMERSCRAPCSIQKEDPTFKEKYRFHAINGYVMDTLPGLVVAQDQKIRWYLLSMGSNENIHSIHFSGQVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKVGIWRIECLIGEHLQAGMSTLFLVYSPKCQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPISWIKVDLLAPMIIHSIMTQGARQKLSSLYISQFIIMYSLDGKKWQSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQVTASSYLNSMFGTWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFQKTMKVTGIATQGVKSLLTSMYVKEFLVSSSQDGHNWALFLHNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWAHQIALRLEVLGCEAQQVY

Target ORF information:

RefSeq Version	XM_019824517.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus coagulation factor VIII (F8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019824517.1

ORF Insert Sequence:

ATGTACAGAA AGACTGTGTT TGTAGAGTTT ACAGATCGCC TTTTCAACAT TGCCAAGCCC 
AGGCCACCAT GGATGGGTCT GCTGGGTCCT ACCATCCGGG CCGAAGTTTT TGACACAGTG 
GTCATTACAC TCAAGAACAT GGCTTCTCAC CCTGTCAGTC TTCATGCTGT TGGTGTATCC 
TATTGGAAAG CTTCTGAAGG TGCTGAATAT GAGGACCAGA CCAGCCAGAA GGAGAAGGAA 
GATGATAAAG TCTTTCCTGG TGAGAGTCAT ACCTACGTCT GGCAGGTCCT GAAAGAGAAT 
GGACCGATGG CCTCGGATCC TCCATGTCTC ACCTACTCAT ATTTTTCTCA TGTGGACCTG 
GTGAAAGACC TGAATTCAGG CCTCATTGGA GCCCTGCTGG TTTGCAAAGA AGGAAGTCTG 
GCTGAAGAAA GGACACAGAC CTTGCATGAA TTTGTACTCC TGTTTGCTGT ATTTGATGAA 
GGAAAAAGCT GGCACTCATC TGCTCAGGCC CAGCATGAAA TGCACACGGT CAACGGCTAT 
GTCAACAGGT CTCTGCCAGG TCTGACTGGA TGCCACAAGA AATCAGTCTA TTGGCATGTG 
ATTGGAATGG GCACCACCCC CGAAGTGCAC TCAATTTTTC TTGAAGGTCA CACATTTCTC 
ATCAGAAACC ACCGCCAGGC CTCCTTGGAG ATCTCACCAA TAACTTTCCT CACTGCTCAG 
ACATTCCTGA TGGACCTTGG CCAGTTTCTA CTGTTTTGTC ACATCTCTTC CCACCAGCAT 
GATGGCATGG AAGCTTATGT CAAAGTAGAT AGCTGCCCAG AGGAACCCCA GCTACGGATG 
AAAACTAATG AAAAAGAGGA AGATTATGAT GATGATCTTT ATGATTCTGA CATGGACATG 
GTTAGGTTTG ATGGCGACAG CCCTTCTCCC TTTATTCAAA TCCGCTCAGT TGCCAAGAAG 
CATCCTAAAA CTTGGATCCA CTATATTGCC GCCGAAGAGG AGGACTGGGA CTATGCTCCC 
TCGGCCCCCA CCCCCATTGA CAGAAGTTAT AAACATCTAT ATTTGAACAG TGGTCCTCAG 
CGGATTGGTA GGAAGTACAA AAAAGTTCGA TTTATGGCAT ACACAGATGA AACATTTAAG 
ACTCGTGAAG CTATTCAGTA TGAATCAGGA ATCCTAGGAC CTTTACTTTA TGGAGAAGTT 
GGAGACACAC TGTTGATTAT ATTTAAAAAT CAAGCAAGCC GACCATATAA CATCTACCCT 
CATGGAATCA CTGACGTCAG TCCTTTGTAC TCAGGGAGAT TGCCTAAAGG TATGAAACAT 
TTGAAAGATA TGCCAATTAT GCCAGGAGAG ATATTCAAGT ATAAATGGAC AGTGACTGTA 
GAAGATGGGC CGACTAAATC AGATCCTCGG TGCCTGACTC GATATTACTC GAGCTTCATT 
AATCCAGAGA GAGATCTAGC TTCAGGACTC ATTGGCCCTC TCCTCATATG CTACAAAGGA 
TCTGTAGATC AAAGAGGAAA CCAGATGATG TCAGACAAGA GAAACGTCAT CCTGTTTTCT 
GTATTTGATG AGAACCAAAG CTGGTACCTT GCAGAGAATA TGAAGCGCTT CCTCCCGAAT 
GCAGATGCAG TGCCGCCCCA CGACCCGGAG CTCCAGGTCT CTAACATCAT GCACAGCATC 
AACGGCTATG TTTTTGACAA CTTGCAGCTG TCAGTTTGTT TGCATGAGGT GGCATACTGG 
TACATTCTAA GTGTTGGAGC ACAAACTGAC TTCCTGTCTG TCTTCTTCTC TGGATACACC 
TTCAAACACA AAATGGTCTA TGAAGACACA CTTACCCTAT TCCCATTCTC AGGAGAAACT 
GTCTTCATGT CAATGGAAAA TCCAGGTCTA TGGGTGCTGG GGTGCCACAA CTCAGACTTT 
CGGAACAGAG GCATGACAGC TTTACTGAAG GTTTCTAGTT GTAATGGGAA CATTGATGAT 
TATGATGAGG ATAGATATGA AGATATTCCA GCCTCCCTCC TGAATGAAAA CAATGTCATT 
GAACCCAGAA GCTTCTCCCA GAATTCAAGA CATCATAGCC CTAGGCAAAA GCAATTCAAA 
GCCACCACAA CTCCAGAAAA TGACATAGAG AAGATTGACC CTCAGCCTGG AGAAAGAACG 
CAGCTGCTTA ACGTACAAAG TGTCTCCTCT AGTGATTTGC TGATGCTTCT GGGACAGAAT 
CCTACTCCAC GTGGATTACT CTTATCTGAT TTCCAGGAAG CCACACATGA AGCAGATGAT 
CATTTACTTG GAGCTATAGA AAGACACAAG GGCCCATCTG CAGTGGCAGA TCTCAGACCA 
GAGCTCCATC ACAGTGGGGA CAGAGTATTT ACTCCTGAGC CAAAACTAAA ATTAAGATTA 
AATGAGAATT TGGGGACAAC TGTAACAGCA GAGTTGAAGA ACCTTGATTT AAAAGTTTCT 
AGTTCATCAG ACAACCTAAC AGCTTCACCA ACAATTCCAT CAGACAAGTT GGCAGCAGGT 
ACTGAAAAGA CAGGTTCCTT AGGACCCCCA AATATGTCAA TTCACTTAAA TAGTCATTTA 
GGTACTATTG TATTTGGCAA AAATTCATCT CACTCTATTG ACTCTGGTGT ACCTTTGGGC 
TCGAGTGAAG GAGACAATGA TTCCAAGTTG TTAGAAGCAG CTTTAATGAA TAGTCAAGAA 
AGTTCACTGG GAGAAAATGT ATTATCAGTG GAGAGAAATG GGTTATTTAA AGAAGACAGA 
GTTCATGGAC CTGCTTCATT AATTAAAGAT AATGCTTTAT TCAAAGTTAA TATCTCTTTG 
ATAAAGACAA ACAACGCAAC AATTAACTCA ACAACTAATA GCAAAAATCA TGTTGATGTC 
CCAACATTAT TAACTGAGAA CAGTACATCA GTCTGGAAAG ATACTATATT AGAAAGTAAT 
GCTGAGTTTC AAGAAATGAC TTCTTTGCTT CATAATGAAA CATTTATGGA CAGAAATATT 
ACAGCTTTGG AGCTAAAGCA TGTGTCAAAT AAAACTACTT CATCAAAAAA TGTGGAAGTG 
GTCTACCAAA ACAAAGAGGG CTCGGTGCCA CTAGGTGCAG AAAATCCAGG TCTCTCATTC 
TTCAAGACAC TGTTCTCACC AGATTCAGCA AAGTGGATAA AATGGACCCA TGGCGAGAAC 
TCCCTAAGCT CTGGGCAAAG GCCCATTCCA ACACAATTAA CATCTTTAGG ATCAGAAAAA 
TCTGTGAAAG ATCAGAAGTT TTTGTCAGAA AAGAAGGTGG TAGTAGGAGA AGATGAATTT 
ACAAAGGACA CAGAACTCAA AGAGATGAGT TTTCCAAACA GCAAGAGCAT GTTTTTTACT 
AACTTGCCCA ATGTCCAAGA TAATGGTCCT TACAATCAAG AAGAAAAATC TCAGGAAGAG 
ATAGAAAGAA AGGAGAAATT AACCCAAGAA AATGTGGTTT TGCCTCAGGA ATATACAGTA 
GCTGACACTA ATAATTCCCT GAAGAACCTT TTCTTACTAA GCACTAAGCA AAATGTGGAA 
GGTTTAGATG AGGGGCCATA TACTCCAATA CTTCAAGACA CCAGGTCATT AAATGATTCA 
GCACATAGAG CAGGGATTCA CATAGTCCAT TTCTCAAAAA TAAGGGAGGA AGAAAACTTG 
GAAGGTTTGG GAAATCAAAC AAAGGAAATG GCAGAGAGGT TTTCAAGCAC TCCAAGGATG 
TCTCCTAATC CAAGTCAGCA TAATATTCTC ACTCAGCGTG GGAAGCGGGC CTTGAAACAA 
TCCAGACTCT CACTAGAAGA AACTAAGCTT GCAAGGGGGG TAATTTTGAA TGACACCTCA 
ACCCAGTCAT CCAAAAACAT GAACTTTTTG ATGCATGGAA CCCTCACACA GATAGAGGAC 
AATGAGAAAG AAAAAGGGGC TGCCACTCAG TCCCTCCAAT CAGATTGTTC TATGGGGAAT 
CAGGGCACCA TTCAAATGAA TGACTCTGCA TTACCCATTG CAAAGGAATC AGCATTTCCA 
TCAATCAGAC ATACAGATCT GACCAAGATC CCATCCCAGG ACAACTCTTC TCATCTTCCA 
GTATCAGCCT GCACTTATAC CTTCAGGGAG AGTTCTGGGG TCCAAAAAAG CACTCATTTC 
TCACAAGGAG CCAAGAGAAA TAAACTTTCT GTAGCCTTCC TAACCTCAGA AATGATTGTA 
GGTCAAGGAA AGTTCAGCTC CCTAGGGAAA AGTGCCATGA ACCAACGCAT GTCCCAGAAA 
CGTGAAAACA CTCTTTTGCA ACAAGGCTTG TCTGAAGCAT CTGGCAAAGT TGAATTACTC 
CCTAAAGCTC ATGTTCATCA AGAAGACTCT TTCCCTACAA AAACTAGCAA TGATTCTTCT 
GGCCACCTGG ATCTTATGAA GAAGATCTTC CTTCGGAAAA CACAGGGACC TAAGAAAATA 
AATGGACCCG GAAAAGTGCC CTTCCTGAAA TGGACAACAG AGAGCTCTGA AAAGATTCCC 
TCTGAGCTGC TGGGTCCCCT TGCTTGGGAT AACAACAATG CCACCCAGAT ACCGAGAGAA 
GAGTGGAAAT CCCAAAAGTC ACAGAAAAAC ATAGCTTTTA AGAGCAAGGA CACCATTTTG 
CCCTTGGGCC CTTGTGAAAA CAGTCCTTCA GTAGCAGCAA TAAATGAAGG GCAAGGTAAA 
CTCCAAAGAG AAGCCACCAG GTCAAAGCAA GGAGAGACTG GAAGATTGTG CTCTCAGAAC 
CCACCGGTCT CAAAACGCCA TCAAAGAGAA ATAACCCTTA CTACTCTTCA GTCAGAGGAA 
GACAAAATTG AGTATGATGA TACGTTTTCA ATTGAAATGA AGAGAGAAGA TTTTGACATC 
TATGGTGAAG ATGAAAATCA GGGCCTCCGC AGCTTTCAAA AGAGAACACG ACACTATTTC 
ATTGCTGCAG TGGAGCGTCT CTGGGATTAT GGGATGAGTA GATCTCCCCA TGCACTAAGA 
AACAGGGCTC CAGGTGGGGA TGTCCCTCAG TTCAAGAAGG TGGTTTTCCA GGAATTTACT 
GATGGATCCT TCACTCAGCC CTTATACCGT GGAGAGCTGA ATGAACATTT GGGACTCTTG 
GGGCCATATA TAAGAGCAGA AGTTGAAGAC AATATCATGG TAACTTTCAA AAACCAGGCT 
TCTCGTCCCT ACTCCTTCTA TTCTAGCCTG ATTTCTTATG AGGAAGATCA GAGGCAAGGA 
GCAGAACCTA GAAAAAAGTT TGTCAACCCT AATGAAACCA GAGTTTACTT CTGGAAAGTA 
CAACATCAGA TGGCACCCAC TGAAGATGAG TTTGACTGCA AGGCTTGGGC TTATTTTTCT 
GATGTTGATT TGGAAAAAGA CATGCACTCG GGCTTGATTG GACCCCTTCT GATCTGCCGC 
AGTAACACAC TGAGCCCCGC TCACGGGAGA CAAGTGACAG TGCAGGAGTT TGCTCTGTTT 
TTCACCATTT TTGATGAGAC TAAGAGCTGG TACTTCACGG AAAACATGGA AAGGAGCTGT 
AGAGCCCCCT GCAGTATCCA GAAGGAGGAT CCCACTTTTA AAGAAAAGTA CCGCTTCCAC 
GCAATCAACG GCTATGTGAT GGACACACTA CCCGGCTTGG TAGTGGCTCA GGATCAGAAG 
ATCCGATGGT ATCTGCTCAG CATGGGGAGC AATGAAAACA TTCATTCTAT TCATTTCAGT 
GGGCAGGTGT TCACTGTACG GAAAAAAGAG GAATATAAGA TGGCAGTCTA CAACCTGTAT 
CCAGGTGTTT TTGAGACAGT GGAAATGCTA CCATCCAAAG TTGGAATTTG GCGGATAGAA 
TGCCTAATTG GCGAACACCT GCAAGCTGGG ATGAGCACTC TCTTTCTGGT ATACAGCCCG 
AAGTGTCAGA TTCCACTGGG AATGGCTTCT GGACGCATTA GAGATTTCCA GATCACAGCT 
TCAGGACAGT ATGGACAGTG GGCCCCAAAG CTGGCCAGAC TTCACTATTC TGGATCAATC 
AATGCCTGGA GCACCAAGGA CCCCATTTCC TGGATCAAGG TGGATCTGTT GGCACCAATG 
ATCATTCACA GCATCATGAC CCAGGGCGCC CGTCAGAAGT TGTCCAGCCT GTACATCTCT 
CAGTTTATCA TCATGTATAG TCTTGATGGA AAGAAGTGGC AGAGTTATCG AGGGAATTCC 
ACTGGGACCT TAATGGTGTT CTTTGGCAAT GTGGATTCAT CTGGGATAAA ACACAATATT 
TTTAACCCTC CGATTATTGC TCGGTACATC CGTTTGCACC CAACGCATTA CAGCATCCGC 
AGCACTCTCC GCATGGAGTT GATGGGCTGT GATTTAAACA GCTGCAGCAT GCCACTGGGA 
ATGGAGAGTA AAGCAATATC AGACGCACAG GTCACCGCCT CGTCCTACTT AAACAGCATG 
TTTGGCACTT GGTCCCCTTC ACAAGCCCGG CTGCACCTCC AGGGGAGGAC GAATGCCTGG 
AGGCCTCAGG CAAATAATCC AAAAGAGTGG CTGCAAGTGG ACTTCCAGAA GACAATGAAA 
GTCACAGGAA TAGCCACCCA GGGGGTGAAA TCACTCCTTA CCAGCATGTA TGTGAAGGAG 
TTCCTCGTTT CCAGTAGTCA AGATGGCCAT AACTGGGCTC TGTTTCTTCA CAATGGCAAA 
GTCAAGGTTT TTCAGGGAAA TCAAGACTCC TTCACACCCG TGGTGAACTC TCTAGACCCC 
CCACTACTGA CTCGCTACCT TAGAATTCAC CCCCAGAGCT GGGCACACCA GATCGCCCTG 
AGGCTGGAGG TTCTGGGCTG TGAGGCCCAG CAGGTATACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F8 ( XM_019824518.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019680077.1 Felis catus coagulation factor VIII (F8), transcript variant X3, mRNA.

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CloneID	OFb58141
Clone ID Related Accession (Same CDS sequence)	XM_019824518.1
Accession Version	XM_019824518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	coagulation factor VIII isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018741.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC ACCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTATTGGAA AGCTTCTGAA 
GGTGCTGAAT ATGAGGACCA GACCAGCCAG AAGGAGAAGG AAGATGATAA AGTCTTTCCT 
GGTGAGAGTC ATACCTACGT CTGGCAGGTC CTGAAAGAGA ATGGACCGAT GGCCTCGGAT 
CCTCCATGTC TCACCTACTC ATATTTTTCT CATGTGGACC TGGTGAAAGA CCTGAATTCA 
GGCCTCATTG GAGCCCTGCT GGTTTGCAAA GAAGGAAGTC TGGCTGAAGA AAGGACACAG 
ACCTTGCATG AATTTGTACT CCTGTTTGCT GTATTTGATG AAGGAAAAAG CTGGCACTCA 
TCTGCTCAGG CCCAGCATGA AATGCACACG GTCAACGGCT ATGTCAACAG GTCTCTGCCA 
GGTCTGACTG GATGCCACAA GAAATCAGTC TATTGGCATG TGATTGGAAT GGGCACCACC 
CCCGAAGTGC ACTCAATTTT TCTTGAAGGT CACACATTTC TCATCAGAAA CCACCGCCAG 
GCCTCCTTGG AGATCTCACC AATAACTTTC CTCACTGCTC AGACATTCCT GATGGACCTT 
GGCCAGTTTC TACTGTTTTG TCACATCTCT TCCCACCAGC ATGATGGCAT GGAAGCTTAT 
GTCAAAGTAG ATAGCTGCCC AGAGGAACCC CAGCTACGGA TGAAAACTAA TGAAAAAGAG 
GAAGATTATG ATGATGATCT TTATGATTCT GACATGGACA TGGTTAGGTT TGATGGCGAC 
AGCCCTTCTC CCTTTATTCA AATCCGCTCA GTTGCCAAGA AGCATCCTAA AACTTGGATC 
CACTATATTG CCGCCGAAGA GGAGGACTGG GACTATGCTC CCTCGGCCCC CACCCCCATT 
GACAGAAGTT ATAAACATCT ATATTTGAAC AGTGGTCCTC AGCGGATTGG TAGGAAGTAC 
AAAAAAGTTC GATTTATGGC ATACACAGAT GAAACATTTA AGACTCGTGA AGCTATTCAG 
TATGAATCAG GAATCCTAGG ACCTTTACTT TATGGAGAAG TTGGAGACAC ACTGTTGATT 
ATATTTAAAA ATCAAGCAAG CCGACCATAT AACATCTACC CTCATGGAAT CACTGACGTC 
AGTCCTTTGT ACTCAGGGAG ATTGCCTAAA GGTATGAAAC ATTTGAAAGA TATGCCAATT 
ATGCCAGGAG AGATATTCAA GTATAAATGG ACAGTGACTG TAGAAGATGG GCCGACTAAA 
TCAGATCCTC GGTGCCTGAC TCGATATTAC TCGAGCTTCA TTAATCCAGA GAGAGATCTA 
GCTTCAGGAC TCATTGGCCC TCTCCTCATA TGCTACAAAG GATCTGTAGA TCAAAGAGGA 
AACCAGATGA TGTCAGACAA GAGAAACGTC ATCCTGTTTT CTGTATTTGA TGAGAACCAA 
AGCTGGTACC TTGCAGAGAA TATGAAGCGC TTCCTCCCGA ATGCAGATGC AGTGCCGCCC 
CACGACCCGG AGCTCCAGGT CTCTAACATC ATGCACAGCA TCAACGGCTA TGTTTTTGAC 
AACTTGCAGC TGTCAGTTTG TTTGCATGAG GTGGCATACT GGTACATTCT AAGTGTTGGA 
GCACAAACTG ACTTCCTGTC TGTCTTCTTC TCTGGATACA CCTTCAAACA CAAAATGGTC 
TATGAAGACA CACTTACCCT ATTCCCATTC TCAGGAGAAA CTGTCTTCAT GTCAATGGAA 
AATCCAGGTC TATGGGTGCT GGGGTGCCAC AACTCAGACT TTCGGAACAG AGGCATGACA 
GCTTTACTGA AGGTTTCTAG TTGTAATGGG AACATTGATG ATTATGATGA GGATAGATAT 
GAAGATATTC CAGCCTCCCT CCTGAATGAA AACAATGTCA TTGAACCCAG AAGCTTCTCC 
CAGAATTCAA GACATCATAG CCCTAGGCAA AAGCAATTCA AAGCCACCAC AACTCCAGAA 
AATGACATAG AGAAGATTGA CCCTCAGCCT GGAGAAAGAA CGCAGCTGCT TAACGTACAA 
AGTGTCTCCT CTAGTGATTT GCTGATGCTT CTGGGACAGA ATCCTACTCC ACGTGGATTA 
CTCTTATCTG ATTTCCAGGA AGCCACACAT GAAGCAGATG ATCATTTACT TGGAGCTATA 
GAAAGACACA AGGGCCCATC TGCAGTGGCA GATCTCAGAC CAGAGCTCCA TCACAGTGGG 
GACAGAGTAT TTACTCCTGA GCCAAAACTA AAATTAAGAT TAAATGAGAA TTTGGGGACA 
ACTGTAACAG CAGAGTTGAA GAACCTTGAT TTAAAAGTTT CTAGTTCATC AGACAACCTA 
ACAGCTTCAC CAACAATTCC ATCAGACAAG TTGGCAGCAG GTACTGAAAA GACAGGTTCC 
TTAGGACCCC CAAATATGTC AATTCACTTA AATAGTCATT TAGGTACTAT TGTATTTGGC 
AAAAATTCAT CTCACTCTAT TGACTCTGGT GTACCTTTGG GCTCGAGTGA AGGAGACAAT 
GATTCCAAGT TGTTAGAAGC AGCTTTAATG AATAGTCAAG AAAGTTCACT GGGAGAAAAT 
GTATTATCAG TGGAGAGAAA TGGGTTATTT AAAGAAGACA GAGTTCATGG ACCTGCTTCA 
TTAATTAAAG ATAATGCTTT ATTCAAAGTT AATATCTCTT TGATAAAGAC AAACAACGCA 
ACAATTAACT CAACAACTAA TAGCAAAAAT CATGTTGATG TCCCAACATT ATTAACTGAG 
AACAGTACAT CAGTCTGGAA AGATACTATA TTAGAAAGTA ATGCTGAGTT TCAAGAAATG 
ACTTCTTTGC TTCATAATGA AACATTTATG GACAGAAATA TTACAGCTTT GGAGCTAAAG 
CATGTGTCAA ATAAAACTAC TTCATCAAAA AATGTGGAAG TGGTCTACCA AAACAAAGAG 
GGCTCGGTGC CACTAGGTGC AGAAAATCCA GGTCTCTCAT TCTTCAAGAC ACTGTTCTCA 
CCAGATTCAG CAAAGTGGAT AAAATGGACC CATGGCGAGA ACTCCCTAAG CTCTGGGCAA 
AGGCCCATTC CAACACAATT AACATCTTTA GGATCAGAAA AATCTGTGAA AGATCAGAAG 
TTTTTGTCAG AAAAGAAGGT GGTAGTAGGA GAAGATGAAT TTACAAAGGA CACAGAACTC 
AAAGAGATGA GTTTTCCAAA CAGCAAGAGC ATGTTTTTTA CTAACTTGCC CAATGTCCAA 
GATAATGGTC CTTACAATCA AGAAGAAAAA TCTCAGGAAG AGATAGAAAG AAAGGAGAAA 
TTAACCCAAG AAAATGTGGT TTTGCCTCAG GAATATACAG TAGCTGACAC TAATAATTCC 
CTGAAGAACC TTTTCTTACT AAGCACTAAG CAAAATGTGG AAGGTTTAGA TGAGGGGCCA 
TATACTCCAA TACTTCAAGA CACCAGGTCA TTAAATGATT CAGCACATAG AGCAGGGATT 
CACATAGTCC ATTTCTCAAA AATAAGGGAG GAAGAAAACT TGGAAGGTTT GGGAAATCAA 
ACAAAGGAAA TGGCAGAGAG GTTTTCAAGC ACTCCAAGGA TGTCTCCTAA TCCAAGTCAG 
CATAATATTC TCACTCAGCG TGGGAAGCGG GCCTTGAAAC AATCCAGACT CTCACTAGAA 
GAAACTAAGC TTGCAAGGGG GGTAATTTTG AATGACACCT CAACCCAGTC ATCCAAAAAC 
ATGAACTTTT TGATGCATGG AACCCTCACA CAGATAGAGG ACAATGAGAA AGAAAAAGGG 
GCTGCCACTC AGTCCCTCCA ATCAGATTGT TCTATGGGGA ATCAGGGCAC CATTCAAATG 
AATGACTCTG CATTACCCAT TGCAAAGGAA TCAGCATTTC CATCAATCAG ACATACAGAT 
CTGACCAAGA TCCCATCCCA GGACAACTCT TCTCATCTTC CAGTATCAGC CTGCACTTAT 
ACCTTCAGGG AGAGTTCTGG GGTCCAAAAA AGCACTCATT TCTCACAAGG AGCCAAGAGA 
AATAAACTTT CTGTAGCCTT CCTAACCTCA GAAATGATTG TAGGTCAAGG AAAGTTCAGC 
TCCCTAGGGA AAAGTGCCAT GAACCAACGC ATGTCCCAGA AACGTGAAAA CACTCTTTTG 
CAACAAGGCT TGTCTGAAGC ATCTGGCAAA GTTGAATTAC TCCCTAAAGC TCATGTTCAT 
CAAGAAGACT CTTTCCCTAC AAAAACTAGC AATGATTCTT CTGGCCACCT GGATCTTATG 
AAGAAGATCT TCCTTCGGAA AACACAGGGA CCTAAGAAAA TAAATGGACC CGGAAAAGTG 
CCCTTCCTGA AATGGACAAC AGAGAGCTCT GAAAAGATTC CCTCTGAGCT GCTGGGTCCC 
CTTGCTTGGG ATAACAACAA TGCCACCCAG ATACCGAGAG AAGAGTGGAA ATCCCAAAAG 
TCACAGAAAA ACATAGCTTT TAAGAGCAAG GACACCATTT TGCCCTTGGG CCCTTGTGAA 
AACAGTCCTT CAGTAGCAGC AATAAATGAA GGGCAAGGTA AACTCCAAAG AGAAGCCACC 
AGGTCAAAGC AAGGAGAGAC TGGAAGATTG TGCTCTCAGA ACCCACCGGT CTCAAAACGC 
CATCAAAGAG AAATAACCCT TACTACTCTT CAGTCAGAGG AAGACAAAAT TGAGTATGAT 
GATACGTTTT CAATTGAAAT GAAGAGAGAA GATTTTGACA TCTATGGTGA AGATGAAAAT 
CAGGGCCTCC GCAGCTTTCA AAAGAGAACA CGACACTATT TCATTGCTGC AGTGGAGCGT 
CTCTGGGATT ATGGGATGAG TAGATCTCCC CATGCACTAA GAAACAGGGC TCCAGGTGGG 
GATGTCCCTC AGTTCAAGAA GGTGGTTTTC CAGGAATTTA CTGATGGATC CTTCACTCAG 
CCCTTATACC GTGGAGAGCT GAATGAACAT TTGGGACTCT TGGGGCCATA TATAAGAGCA 
GAAGTTGAAG ACAATATCAT GGTAACTTTC AAAAACCAGG CTTCTCGTCC CTACTCCTTC 
TATTCTAGCC TGATTTCTTA TGAGGAAGAT CAGAGGCAAG GAGCAGAACC TAGAAAAAAG 
TTTGTCAACC CTAATGAAAC CAGAGTTTAC TTCTGGAAAG TACAACATCA GATGGCACCC 
ACTGAAGATG AGTTTGACTG CAAGGCTTGG GCTTATTTTT CTGATGTTGA TTTGGAAAAA 
GACATGCACT CGGGCTTGAT TGGACCCCTT CTGATCTGCC GCAGTAACAC ACTGAGCCCC 
GCTCACGGGA GACAAGTGAC AGTGCAGGAG TTTGCTCTGT TTTTCACCAT TTTTGATGAG 
ACTAAGAGCT GGTACTTCAC GGAAAACATG GAAAGGAGCT GTAGAGCCCC CTGCAGTATC 
CAGAAGGAGG ATCCCACTTT TAAAGAAAAG TACCGCTTCC ACGCAATCAA CGGCTATGTG 
ATGGACACAC TACCCGGCTT GGTAGTGGCT CAGGATCAGA AGATCCGATG GTATCTGCTC 
AGCATGGGGA GCAATGAAAA CATTCATTCT ATTCATTTCA GTGGGCAGGT GTTCACTGTA 
CGGAAAAAAG AGGAATATAA GATGGCAGTC TACAACCTGT ATCCAGGTGT TTTTGAGACA 
GTGGAAATGC TACCATCCAA AGTTGGAATT TGGCGGATAG AATGCCTAAT TGGCGAACAC 
CTGCAAGCTG GGATGAGCAC TCTCTTTCTG GTATACAGCC CGAAGTGTCA GATTCCACTG 
GGAATGGCTT CTGGACGCAT TAGAGATTTC CAGATCACAG CTTCAGGACA GTATGGACAG 
TGGGCCCCAA AGCTGGCCAG ACTTCACTAT TCTGGATCAA TCAATGCCTG GAGCACCAAG 
GACCCCATTT CCTGGATCAA GGTGGATCTG TTGGCACCAA TGATCATTCA CAGCATCATG 
ACCCAGGGCG CCCGTCAGAA GTTGTCCAGC CTGTACATCT CTCAGTTTAT CATCATGTAT 
AGTCTTGATG GAAAGAAGTG GCAGAGTTAT CGAGGGAATT CCACTGGGAC CTTAATGGTG 
TTCTTTGGCA ATGTGGATTC ATCTGGGATA AAACACAATA TTTTTAACCC TCCGATTATT 
GCTCGGTACA TCCGTTTGCA CCCAACGCAT TACAGCATCC GCAGCACTCT CCGCATGGAG 
TTGATGGGCT GTGATTTAAA CAGCTGCAGC ATGCCACTGG GAATGGAGAG TAAAGCAATA 
TCAGACGCAC AGGTCACCGC CTCGTCCTAC TTAAACAGCA TGTTTGGCAC TTGGTCCCCT 
TCACAAGCCC GGCTGCACCT CCAGGGGAGG ACGAATGCCT GGAGGCCTCA GGCAAATAAT 
CCAAAAGAGT GGCTGCAAGT GGACTTCCAG AAGACAATGA AAGTCACAGG AATAGCCACC 
CAGGGGGTGA AATCACTCCT TACCAGCATG TATGTGAAGG AGTTCCTCGT TTCCAGTAGT 
CAAGATGGCC ATAACTGGGC TCTGTTTCTT CACAATGGCA AAGTCAAGGT TTTTCAGGGA 
AATCAAGACT CCTTCACACC CGTGGTGAAC TCTCTAGACC CCCCACTACT GACTCGCTAC 
CTTAGAATTC ACCCCCAGAG CTGGGCACAC CAGATCGCCC TGAGGCTGGA GGTTCTGGGC 
TGTGAGGCCC AGCAGGTATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019680077.1
CDS	259..6942
Translation	MASHPVSLHAVGVSYWKASEGAEYEDQTSQKEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYFSHVDLVKDLNSGLIGALLVCKEGSLAEERTQTLHEFVLLFAVFDEGKSWHSSAQAQHEMHTVNGYVNRSLPGLTGCHKKSVYWHVIGMGTTPEVHSIFLEGHTFLIRNHRQASLEISPITFLTAQTFLMDLGQFLLFCHISSHQHDGMEAYVKVDSCPEEPQLRMKTNEKEEDYDDDLYDSDMDMVRFDGDSPSPFIQIRSVAKKHPKTWIHYIAAEEEDWDYAPSAPTPIDRSYKHLYLNSGPQRIGRKYKKVRFMAYTDETFKTREAIQYESGILGPLLYGEVGDTLLIIFKNQASRPYNIYPHGITDVSPLYSGRLPKGMKHLKDMPIMPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPERDLASGLIGPLLICYKGSVDQRGNQMMSDKRNVILFSVFDENQSWYLAENMKRFLPNADAVPPHDPELQVSNIMHSINGYVFDNLQLSVCLHEVAYWYILSVGAQTDFLSVFFSGYTFKHKMVYEDTLTLFPFSGETVFMSMENPGLWVLGCHNSDFRNRGMTALLKVSSCNGNIDDYDEDRYEDIPASLLNENNVIEPRSFSQNSRHHSPRQKQFKATTTPENDIEKIDPQPGERTQLLNVQSVSSSDLLMLLGQNPTPRGLLLSDFQEATHEADDHLLGAIERHKGPSAVADLRPELHHSGDRVFTPEPKLKLRLNENLGTTVTAELKNLDLKVSSSSDNLTASPTIPSDKLAAGTEKTGSLGPPNMSIHLNSHLGTIVFGKNSSHSIDSGVPLGSSEGDNDSKLLEAALMNSQESSLGENVLSVERNGLFKEDRVHGPASLIKDNALFKVNISLIKTNNATINSTTNSKNHVDVPTLLTENSTSVWKDTILESNAEFQEMTSLLHNETFMDRNITALELKHVSNKTTSSKNVEVVYQNKEGSVPLGAENPGLSFFKTLFSPDSAKWIKWTHGENSLSSGQRPIPTQLTSLGSEKSVKDQKFLSEKKVVVGEDEFTKDTELKEMSFPNSKSMFFTNLPNVQDNGPYNQEEKSQEEIERKEKLTQENVVLPQEYTVADTNNSLKNLFLLSTKQNVEGLDEGPYTPILQDTRSLNDSAHRAGIHIVHFSKIREEENLEGLGNQTKEMAERFSSTPRMSPNPSQHNILTQRGKRALKQSRLSLEETKLARGVILNDTSTQSSKNMNFLMHGTLTQIEDNEKEKGAATQSLQSDCSMGNQGTIQMNDSALPIAKESAFPSIRHTDLTKIPSQDNSSHLPVSACTYTFRESSGVQKSTHFSQGAKRNKLSVAFLTSEMIVGQGKFSSLGKSAMNQRMSQKRENTLLQQGLSEASGKVELLPKAHVHQEDSFPTKTSNDSSGHLDLMKKIFLRKTQGPKKINGPGKVPFLKWTTESSEKIPSELLGPLAWDNNNATQIPREEWKSQKSQKNIAFKSKDTILPLGPCENSPSVAAINEGQGKLQREATRSKQGETGRLCSQNPPVSKRHQREITLTTLQSEEDKIEYDDTFSIEMKREDFDIYGEDENQGLRSFQKRTRHYFIAAVERLWDYGMSRSPHALRNRAPGGDVPQFKKVVFQEFTDGSFTQPLYRGELNEHLGLLGPYIRAEVEDNIMVTFKNQASRPYSFYSSLISYEEDQRQGAEPRKKFVNPNETRVYFWKVQHQMAPTEDEFDCKAWAYFSDVDLEKDMHSGLIGPLLICRSNTLSPAHGRQVTVQEFALFFTIFDETKSWYFTENMERSCRAPCSIQKEDPTFKEKYRFHAINGYVMDTLPGLVVAQDQKIRWYLLSMGSNENIHSIHFSGQVFTVRKKEEYKMAVYNLYPGVFETVEMLPSKVGIWRIECLIGEHLQAGMSTLFLVYSPKCQIPLGMASGRIRDFQITASGQYGQWAPKLARLHYSGSINAWSTKDPISWIKVDLLAPMIIHSIMTQGARQKLSSLYISQFIIMYSLDGKKWQSYRGNSTGTLMVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMELMGCDLNSCSMPLGMESKAISDAQVTASSYLNSMFGTWSPSQARLHLQGRTNAWRPQANNPKEWLQVDFQKTMKVTGIATQGVKSLLTSMYVKEFLVSSSQDGHNWALFLHNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWAHQIALRLEVLGCEAQQVY

Target ORF information:

RefSeq Version	XM_019824518.1
Organism	Felis catus(domestic cat)
Definition	Felis catus coagulation factor VIII (F8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019824518.1

ORF Insert Sequence:

ATGGCTTCTC ACCCTGTCAG TCTTCATGCT GTTGGTGTAT CCTATTGGAA AGCTTCTGAA 
GGTGCTGAAT ATGAGGACCA GACCAGCCAG AAGGAGAAGG AAGATGATAA AGTCTTTCCT 
GGTGAGAGTC ATACCTACGT CTGGCAGGTC CTGAAAGAGA ATGGACCGAT GGCCTCGGAT 
CCTCCATGTC TCACCTACTC ATATTTTTCT CATGTGGACC TGGTGAAAGA CCTGAATTCA 
GGCCTCATTG GAGCCCTGCT GGTTTGCAAA GAAGGAAGTC TGGCTGAAGA AAGGACACAG 
ACCTTGCATG AATTTGTACT CCTGTTTGCT GTATTTGATG AAGGAAAAAG CTGGCACTCA 
TCTGCTCAGG CCCAGCATGA AATGCACACG GTCAACGGCT ATGTCAACAG GTCTCTGCCA 
GGTCTGACTG GATGCCACAA GAAATCAGTC TATTGGCATG TGATTGGAAT GGGCACCACC 
CCCGAAGTGC ACTCAATTTT TCTTGAAGGT CACACATTTC TCATCAGAAA CCACCGCCAG 
GCCTCCTTGG AGATCTCACC AATAACTTTC CTCACTGCTC AGACATTCCT GATGGACCTT 
GGCCAGTTTC TACTGTTTTG TCACATCTCT TCCCACCAGC ATGATGGCAT GGAAGCTTAT 
GTCAAAGTAG ATAGCTGCCC AGAGGAACCC CAGCTACGGA TGAAAACTAA TGAAAAAGAG 
GAAGATTATG ATGATGATCT TTATGATTCT GACATGGACA TGGTTAGGTT TGATGGCGAC 
AGCCCTTCTC CCTTTATTCA AATCCGCTCA GTTGCCAAGA AGCATCCTAA AACTTGGATC 
CACTATATTG CCGCCGAAGA GGAGGACTGG GACTATGCTC CCTCGGCCCC CACCCCCATT 
GACAGAAGTT ATAAACATCT ATATTTGAAC AGTGGTCCTC AGCGGATTGG TAGGAAGTAC 
AAAAAAGTTC GATTTATGGC ATACACAGAT GAAACATTTA AGACTCGTGA AGCTATTCAG 
TATGAATCAG GAATCCTAGG ACCTTTACTT TATGGAGAAG TTGGAGACAC ACTGTTGATT 
ATATTTAAAA ATCAAGCAAG CCGACCATAT AACATCTACC CTCATGGAAT CACTGACGTC 
AGTCCTTTGT ACTCAGGGAG ATTGCCTAAA GGTATGAAAC ATTTGAAAGA TATGCCAATT 
ATGCCAGGAG AGATATTCAA GTATAAATGG ACAGTGACTG TAGAAGATGG GCCGACTAAA 
TCAGATCCTC GGTGCCTGAC TCGATATTAC TCGAGCTTCA TTAATCCAGA GAGAGATCTA 
GCTTCAGGAC TCATTGGCCC TCTCCTCATA TGCTACAAAG GATCTGTAGA TCAAAGAGGA 
AACCAGATGA TGTCAGACAA GAGAAACGTC ATCCTGTTTT CTGTATTTGA TGAGAACCAA 
AGCTGGTACC TTGCAGAGAA TATGAAGCGC TTCCTCCCGA ATGCAGATGC AGTGCCGCCC 
CACGACCCGG AGCTCCAGGT CTCTAACATC ATGCACAGCA TCAACGGCTA TGTTTTTGAC 
AACTTGCAGC TGTCAGTTTG TTTGCATGAG GTGGCATACT GGTACATTCT AAGTGTTGGA 
GCACAAACTG ACTTCCTGTC TGTCTTCTTC TCTGGATACA CCTTCAAACA CAAAATGGTC 
TATGAAGACA CACTTACCCT ATTCCCATTC TCAGGAGAAA CTGTCTTCAT GTCAATGGAA 
AATCCAGGTC TATGGGTGCT GGGGTGCCAC AACTCAGACT TTCGGAACAG AGGCATGACA 
GCTTTACTGA AGGTTTCTAG TTGTAATGGG AACATTGATG ATTATGATGA GGATAGATAT 
GAAGATATTC CAGCCTCCCT CCTGAATGAA AACAATGTCA TTGAACCCAG AAGCTTCTCC 
CAGAATTCAA GACATCATAG CCCTAGGCAA AAGCAATTCA AAGCCACCAC AACTCCAGAA 
AATGACATAG AGAAGATTGA CCCTCAGCCT GGAGAAAGAA CGCAGCTGCT TAACGTACAA 
AGTGTCTCCT CTAGTGATTT GCTGATGCTT CTGGGACAGA ATCCTACTCC ACGTGGATTA 
CTCTTATCTG ATTTCCAGGA AGCCACACAT GAAGCAGATG ATCATTTACT TGGAGCTATA 
GAAAGACACA AGGGCCCATC TGCAGTGGCA GATCTCAGAC CAGAGCTCCA TCACAGTGGG 
GACAGAGTAT TTACTCCTGA GCCAAAACTA AAATTAAGAT TAAATGAGAA TTTGGGGACA 
ACTGTAACAG CAGAGTTGAA GAACCTTGAT TTAAAAGTTT CTAGTTCATC AGACAACCTA 
ACAGCTTCAC CAACAATTCC ATCAGACAAG TTGGCAGCAG GTACTGAAAA GACAGGTTCC 
TTAGGACCCC CAAATATGTC AATTCACTTA AATAGTCATT TAGGTACTAT TGTATTTGGC 
AAAAATTCAT CTCACTCTAT TGACTCTGGT GTACCTTTGG GCTCGAGTGA AGGAGACAAT 
GATTCCAAGT TGTTAGAAGC AGCTTTAATG AATAGTCAAG AAAGTTCACT GGGAGAAAAT 
GTATTATCAG TGGAGAGAAA TGGGTTATTT AAAGAAGACA GAGTTCATGG ACCTGCTTCA 
TTAATTAAAG ATAATGCTTT ATTCAAAGTT AATATCTCTT TGATAAAGAC AAACAACGCA 
ACAATTAACT CAACAACTAA TAGCAAAAAT CATGTTGATG TCCCAACATT ATTAACTGAG 
AACAGTACAT CAGTCTGGAA AGATACTATA TTAGAAAGTA ATGCTGAGTT TCAAGAAATG 
ACTTCTTTGC TTCATAATGA AACATTTATG GACAGAAATA TTACAGCTTT GGAGCTAAAG 
CATGTGTCAA ATAAAACTAC TTCATCAAAA AATGTGGAAG TGGTCTACCA AAACAAAGAG 
GGCTCGGTGC CACTAGGTGC AGAAAATCCA GGTCTCTCAT TCTTCAAGAC ACTGTTCTCA 
CCAGATTCAG CAAAGTGGAT AAAATGGACC CATGGCGAGA ACTCCCTAAG CTCTGGGCAA 
AGGCCCATTC CAACACAATT AACATCTTTA GGATCAGAAA AATCTGTGAA AGATCAGAAG 
TTTTTGTCAG AAAAGAAGGT GGTAGTAGGA GAAGATGAAT TTACAAAGGA CACAGAACTC 
AAAGAGATGA GTTTTCCAAA CAGCAAGAGC ATGTTTTTTA CTAACTTGCC CAATGTCCAA 
GATAATGGTC CTTACAATCA AGAAGAAAAA TCTCAGGAAG AGATAGAAAG AAAGGAGAAA 
TTAACCCAAG AAAATGTGGT TTTGCCTCAG GAATATACAG TAGCTGACAC TAATAATTCC 
CTGAAGAACC TTTTCTTACT AAGCACTAAG CAAAATGTGG AAGGTTTAGA TGAGGGGCCA 
TATACTCCAA TACTTCAAGA CACCAGGTCA TTAAATGATT CAGCACATAG AGCAGGGATT 
CACATAGTCC ATTTCTCAAA AATAAGGGAG GAAGAAAACT TGGAAGGTTT GGGAAATCAA 
ACAAAGGAAA TGGCAGAGAG GTTTTCAAGC ACTCCAAGGA TGTCTCCTAA TCCAAGTCAG 
CATAATATTC TCACTCAGCG TGGGAAGCGG GCCTTGAAAC AATCCAGACT CTCACTAGAA 
GAAACTAAGC TTGCAAGGGG GGTAATTTTG AATGACACCT CAACCCAGTC ATCCAAAAAC 
ATGAACTTTT TGATGCATGG AACCCTCACA CAGATAGAGG ACAATGAGAA AGAAAAAGGG 
GCTGCCACTC AGTCCCTCCA ATCAGATTGT TCTATGGGGA ATCAGGGCAC CATTCAAATG 
AATGACTCTG CATTACCCAT TGCAAAGGAA TCAGCATTTC CATCAATCAG ACATACAGAT 
CTGACCAAGA TCCCATCCCA GGACAACTCT TCTCATCTTC CAGTATCAGC CTGCACTTAT 
ACCTTCAGGG AGAGTTCTGG GGTCCAAAAA AGCACTCATT TCTCACAAGG AGCCAAGAGA 
AATAAACTTT CTGTAGCCTT CCTAACCTCA GAAATGATTG TAGGTCAAGG AAAGTTCAGC 
TCCCTAGGGA AAAGTGCCAT GAACCAACGC ATGTCCCAGA AACGTGAAAA CACTCTTTTG 
CAACAAGGCT TGTCTGAAGC ATCTGGCAAA GTTGAATTAC TCCCTAAAGC TCATGTTCAT 
CAAGAAGACT CTTTCCCTAC AAAAACTAGC AATGATTCTT CTGGCCACCT GGATCTTATG 
AAGAAGATCT TCCTTCGGAA AACACAGGGA CCTAAGAAAA TAAATGGACC CGGAAAAGTG 
CCCTTCCTGA AATGGACAAC AGAGAGCTCT GAAAAGATTC CCTCTGAGCT GCTGGGTCCC 
CTTGCTTGGG ATAACAACAA TGCCACCCAG ATACCGAGAG AAGAGTGGAA ATCCCAAAAG 
TCACAGAAAA ACATAGCTTT TAAGAGCAAG GACACCATTT TGCCCTTGGG CCCTTGTGAA 
AACAGTCCTT CAGTAGCAGC AATAAATGAA GGGCAAGGTA AACTCCAAAG AGAAGCCACC 
AGGTCAAAGC AAGGAGAGAC TGGAAGATTG TGCTCTCAGA ACCCACCGGT CTCAAAACGC 
CATCAAAGAG AAATAACCCT TACTACTCTT CAGTCAGAGG AAGACAAAAT TGAGTATGAT 
GATACGTTTT CAATTGAAAT GAAGAGAGAA GATTTTGACA TCTATGGTGA AGATGAAAAT 
CAGGGCCTCC GCAGCTTTCA AAAGAGAACA CGACACTATT TCATTGCTGC AGTGGAGCGT 
CTCTGGGATT ATGGGATGAG TAGATCTCCC CATGCACTAA GAAACAGGGC TCCAGGTGGG 
GATGTCCCTC AGTTCAAGAA GGTGGTTTTC CAGGAATTTA CTGATGGATC CTTCACTCAG 
CCCTTATACC GTGGAGAGCT GAATGAACAT TTGGGACTCT TGGGGCCATA TATAAGAGCA 
GAAGTTGAAG ACAATATCAT GGTAACTTTC AAAAACCAGG CTTCTCGTCC CTACTCCTTC 
TATTCTAGCC TGATTTCTTA TGAGGAAGAT CAGAGGCAAG GAGCAGAACC TAGAAAAAAG 
TTTGTCAACC CTAATGAAAC CAGAGTTTAC TTCTGGAAAG TACAACATCA GATGGCACCC 
ACTGAAGATG AGTTTGACTG CAAGGCTTGG GCTTATTTTT CTGATGTTGA TTTGGAAAAA 
GACATGCACT CGGGCTTGAT TGGACCCCTT CTGATCTGCC GCAGTAACAC ACTGAGCCCC 
GCTCACGGGA GACAAGTGAC AGTGCAGGAG TTTGCTCTGT TTTTCACCAT TTTTGATGAG 
ACTAAGAGCT GGTACTTCAC GGAAAACATG GAAAGGAGCT GTAGAGCCCC CTGCAGTATC 
CAGAAGGAGG ATCCCACTTT TAAAGAAAAG TACCGCTTCC ACGCAATCAA CGGCTATGTG 
ATGGACACAC TACCCGGCTT GGTAGTGGCT CAGGATCAGA AGATCCGATG GTATCTGCTC 
AGCATGGGGA GCAATGAAAA CATTCATTCT ATTCATTTCA GTGGGCAGGT GTTCACTGTA 
CGGAAAAAAG AGGAATATAA GATGGCAGTC TACAACCTGT ATCCAGGTGT TTTTGAGACA 
GTGGAAATGC TACCATCCAA AGTTGGAATT TGGCGGATAG AATGCCTAAT TGGCGAACAC 
CTGCAAGCTG GGATGAGCAC TCTCTTTCTG GTATACAGCC CGAAGTGTCA GATTCCACTG 
GGAATGGCTT CTGGACGCAT TAGAGATTTC CAGATCACAG CTTCAGGACA GTATGGACAG 
TGGGCCCCAA AGCTGGCCAG ACTTCACTAT TCTGGATCAA TCAATGCCTG GAGCACCAAG 
GACCCCATTT CCTGGATCAA GGTGGATCTG TTGGCACCAA TGATCATTCA CAGCATCATG 
ACCCAGGGCG CCCGTCAGAA GTTGTCCAGC CTGTACATCT CTCAGTTTAT CATCATGTAT 
AGTCTTGATG GAAAGAAGTG GCAGAGTTAT CGAGGGAATT CCACTGGGAC CTTAATGGTG 
TTCTTTGGCA ATGTGGATTC ATCTGGGATA AAACACAATA TTTTTAACCC TCCGATTATT 
GCTCGGTACA TCCGTTTGCA CCCAACGCAT TACAGCATCC GCAGCACTCT CCGCATGGAG 
TTGATGGGCT GTGATTTAAA CAGCTGCAGC ATGCCACTGG GAATGGAGAG TAAAGCAATA 
TCAGACGCAC AGGTCACCGC CTCGTCCTAC TTAAACAGCA TGTTTGGCAC TTGGTCCCCT 
TCACAAGCCC GGCTGCACCT CCAGGGGAGG ACGAATGCCT GGAGGCCTCA GGCAAATAAT 
CCAAAAGAGT GGCTGCAAGT GGACTTCCAG AAGACAATGA AAGTCACAGG AATAGCCACC 
CAGGGGGTGA AATCACTCCT TACCAGCATG TATGTGAAGG AGTTCCTCGT TTCCAGTAGT 
CAAGATGGCC ATAACTGGGC TCTGTTTCTT CACAATGGCA AAGTCAAGGT TTTTCAGGGA 
AATCAAGACT CCTTCACACC CGTGGTGAAC TCTCTAGACC CCCCACTACT GACTCGCTAC 
CTTAGAATTC ACCCCCAGAG CTGGGCACAC CAGATCGCCC TGAGGCTGGA GGTTCTGGGC 
TGTGAGGCCC AGCAGGTATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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F8 cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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