FREM2 cDNA ORF clone, Felis catus(domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM2
Entrez Gene ID	101087292
Full Name	FRAS1 related extracellular matrix protein 2
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023250973.1	XP_023106741.1	FRAS1-related extracellular matrix protein 2 isoform X2
XM_003980340.4	XP_003980389.1	FRAS1-related extracellular matrix protein 2
XM_003980340.5	XP_003980389.1	FRAS1-related extracellular matrix protein 2 isoform X1

Pathways from BioSystems
Gene Ontology

The following FREM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb28387	XM_003980340.4 Latest version!	Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFb69998	XM_003980340.5 Latest version!	Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFb69999	XM_023250973.1 Latest version!	Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM2 ( XM_003980340.4 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_003980389.1 Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

Price & Availability↑

CloneID	OFb28387
Clone ID Related Accession (Same CDS sequence)	XM_003980340.4
Accession Version	XM_003980340.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	FRAS1-related extracellular matrix protein 2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018723.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003980340.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGCGG CCAGGACCCC CGGGATCGCC TCCCACGCCG GCGGGGTGGC CAGCCCCACC 
AGTTTTCAGC AGATCCCAAC GCTGCCGCCC CGCCTGGTGT TGCTGCTGCT GCTGCTTCTC 
CTGTCACTGG TAAGCTGCGT CCTGGCACAG CCCGCCGCCC CTGGCCCGGC GTTGCTGTCC 
CCGGATCTCG CGGGGGCTGC AGGGGTCCCC GCCGAGGAGG CCATCGTGCT GGCAAACCGT 
GGGCTCCGGG TGCCCTTTGG CCGTGAGGTC TGGCTGGATC CGCTGCAGGA CCTGGTGCTG 
CAGGTGCAGC CGGGGGACCG CTGCGCGGTG ACCGTGCTAG ACAACGACGC GCTGGCCCAG 
CGGCCCGGAC GCTTGAGTCC CAAGCGCTTC CCGTGCGACT TCGGCCCGAG AGAGGTGCGC 
TACTCGCACC TGGGCGCGCG CAGCCCGTCG CGGGATCGCA TTCGGCTGCA GTTGCGCTAC 
GACGCGCCGG GAGGGGCCGC AGTGCTACCC TTGGTGCTGG AGGTGGAGGT GGTCTTCACC 
CAGCTGGAGG TGGTGACTCG GAACCTGCCC CTAGTCGTGG AGGAGCTGCT GGGGACCAGC 
AACGCCCTGG ACGCGCGGAG CCTGGAGTTC GCCTACCAGC CGGAGACCGA GGAGTGTCGC 
GTAGGCATCC TGTCCGGCTT GAGCGCGCTG CCTCGCTATG GGGAGCTCCT CCACTACCCA 
CAGGCCCCGG GTCCAGCCAG CGAGGGGGGC GCCCCGGAGC CTCTCCTGAT GGACTGCAAA 
GCTTTCCAGG AGCTGGGGGT GCGCTACCGT CACACAGCCC CCAGTCGGTC ACCGAACAGG 
GACTGGGTGC CCATGGTGGT TGAGCTGCGC TCTCGGGGTT CGCCTGTGGG CAGACCCCCT 
TTGAAACGTG AGCACTTCCA GGTGCTGGTG AGAATCCGAG GAGGGGCGGA GAACACCGCA 
CCCAGGCCCA GTTTCGTGGC CATGATGATG ATGGAGGTGG ACCAGTTTGT ACTGACGGCT 
CTGACCCCAG ACATGCTGGC AGCCGAGGAT GCCGAGTCGC CCCCTGACCT GTTGATCTTC 
AACCTCACCT CTCCCTTCCA GCCTGGCCAG GGCTACCTAG TGAGTACTGA TGACCGCAGC 
CTGCCCCTCT CCTCCTTTAC TCAGAGGGAT CTGCGGCTCC TGAAGATTGC CTACCAGCCC 
CCCTCTGAGG ACTCTGACCA GGAGCGTCTC TTTGAACTGG AGTTGGAAGC AGTGGACCCG 
GAAGGAGCAG CCTCAGACCC TTTTGCCTTC ATGGTAGTGG TGAAGCCCAT GAACACACTG 
GCTCCCGTGG TCACCCGGAA CACCGGTCTC ATTCTCTATG AGGGCCAGTC TCGGCCCCTG 
ACGGGCCTTG CAGGCAGTGG ACCGCAGAAC TTGGTCGTCA GCGATGAGGA TGACCTAGAG 
GCAGTGCAGC TAGAGGTGGT GGCTGGGCTC CGCCACGGTC ACCTTGTCCT TCTGGGCGCC 
ACCGATGGCA ACTCTGCCCC TAAGACCTTC ACGGTGGCCG AGCTGGCCGC CGGCCAGGTG 
GTCTACCATC ACGATGACAG AGATGGCTCA CTGAGTGACA ACCTTGTACT TAGAATGGTG 
GACGGAGGAC GCAGGCACCA GGTGCAGTTC TTGTTCCCCA TCACCTTGGT GCCTGTGGAT 
GACCAGCCGC CAGTCCTCAA TGCTAACACG GGGCTGACGC TGGCGGAGGG TGAAACGGCG 
CCCATCCTTC CCCTGGCCCT GAGTGCAACG GACATCGACT CAGATGACCC TCTGCTGCTT 
TTTGTGCTGG AGTCGCCTTC TTCGACCATA GGGCATCTGC TTCTCCGCCA AACCCATCCT 
CCCCGCGAGG AAGAGGAGCT GAGCAGGGGC CCTTGGAGGA AACAGGGAGC GTTCTATGAG 
CAAACGGTGA CGGAGTGGAG GCAGCGGGAC ATAACAGAGG GGAGGCTGTT CTACAGGCAC 
TCCGGGCCCC ATAGTCCTGG GCCAGTGATG GACCAGTTCA CATTCCGGGT CCAGGATAAC 
CATGACCCCC CTAATCAGTC TGGACCACAC AGGTTTGTGA TTCGCATCCA TCCTGTGGAT 
CGCCTCCCTC CGGAGCTGGG CAGCGGCTGT CCCCTTCGGA TGGTGGTGCA AGAATCCCAG 
CTCACGCCGC TGAGGAAAAA GTGGCTGCGC TACACTGACC TGGACACAGA TGACCGAGAG 
CTGCGCTACA CCGTGACCCA GCCCCCCACG GACACAGATG AAAACCACTC ACCAGCCCCC 
CTGGGCACCT TGGTCCTGAC CGACAACCCC TCAGTCGTGG TGGCCCATTT TACCCAAGCC 
CAGATCAACC ACCATAAAAT TGCCTACAGA CCTCCGGATC GAGAACTGGG AGTGGCTGCC 
CGAGTGGCCC AGTTTCAATT CCAGGTGGAG GACCAAGCCG GGAATGTGGC TCCAGGCACC 
TTCACCCTTT ACTTGCAGCC CGTGGACAAC CAGCCGCCTG AGATCCTCAA CACGGGTTTC 
ACCGTTCAGG TGAAGGGCCA CCACATCCTG AGTGAGACTG AGTTACGTGT GAATGATGTA 
GACACCGATG TAGCCCACAT CTCTTTCACT CTCACACGGG CACCCGAACA TGGCCACATG 
AGAGTGTCTG GACAGATACT GCATGTGGGG GAGGTCTTCC GCTTGGAAGA TATAAAACAG 
GGTCGGATTT CCTATGTCCA TAATGGGGAC GGGTCCCTGA CTGACAGCTG CTCCTTGGAG 
GTCAGCGACA GACACCACGT GGTGCCCATC ACTCTCAGAG TGAATGTCCG GCCCGCGGGT 
GATGAGGTGC CCATGCTGAG CCTTCCTGCT GGTACTCTGG GGTCATATCT AGACGTTTTA 
GAAAATGGAG CTGCTGAACT CACTGCCAGT GTTATCAAGG GGACTGATGG GGACACTGAT 
GATTTGATGT TGACGTTCCT CTTGGAAGAC CCACCCTTGT ATGGAGAAAT CCTGGTCAAC 
GGAGTTCCGG CGGAGCAGTT TACCCAAAGG GACATCTTAG AGGGCTCTGT CGTCTATGCC 
CACACAAGCG GTGAGATAGG CCTGTTGCCC AAAGGGGATT CTTTTAACCT GAGTCTGTCA 
GATATGTCTC AAGAATGGGT AATAGGTGGC AGTACTATCC AAGGGGTTAC TGTGTGGGTG 
ACCATCCTCC CTGTCGATAG CCAAGCTCCA GAAATTTCTG TAGGTGAACG GTTTATAGTC 
ATGGAAGGTG ACAAAAGTGT TATAACCTCA ACACATATAA GTGCTGAAGA TATTGATTCC 
CTCAATGATG ACATCTTGTG CACTATAGTT ATTCAGCCTA CCTCTGGTTA TGTTGAAAAC 
ATTTCTCCAG CACCAGGCTC TGAGAAATCA AGAGCAGGAA TTGCCATAAG TGCCTTTACC 
CTGAAAGACC TCAGGCAGAG TCATATTAAC TATGTCCAGA GTGTCCACAA AGGGGTAGAA 
CCTGTGGAAG ACCGGTTTAT ATTTCGTTGC TCTGATGGCA TTAACTTTTC AGAGAGACAT 
TTTTTTCCCA TTATAATCAT TCCCACCAAT GATGAACAGC CAGAAATATT TATGAGAGAA 
TTTATGGTGA TGGAAGGCAT GAGTCTGGTG ATCGATACAC CCATCCTCAA TGCTGCTGAT 
GCTGACATTC CCCCGGATGA TCTAACTTTC ACCATTACCC AATTTCCCAC TCGTGGTCAC 
ATCATGAATC AGCTGATAAA TGGCACAGTG TTGGTTGAAA GCTTCACCTT GGACCAGATC 
ATAGAGAGTT CAAGCATTAT TTACGAGCAT GATGATTCTG AGACCCAGGA GGACAGTTTT 
GTGATTAAAC TAACAGACGG TAAGCACTCG GTGGAAAGGA TGGTCCTCAT TATGGTTATC 
CCAGTTGATG ATGAGACCCC CAGAATGACC ATCAATAATG GTCTAGAAAT AGAAATTGGG 
GAAACCAAAA TTATCAACAA CAAAATATTG ATGGCAACTG ATTTAGATTC TGAAGACAAA 
TCTTTGGTTT ATATTATTCG TTATGGGCCG GGACATGGCT TATTACAGAG ACAAAAACCC 
GCAGGTGCCT TTGAAAATAT CACAGTGGGC ATGAATTTTA CCCAGGATGA AGTGGACAGA 
AACTTAATTC AGTACGTCCA TTTTGGACAA GAGGGCATTC GGGACCTAAT TAAATTTGAT 
GTGACTGATG GAATAAATGC GCTTATAGAT CGCTACTTTT ATGTTTCTGT TGGGAGCGTT 
GACATTGTCT TCCCGGATGT GATAAGCAAG GGAGTATCCT TGAAAGAAGG TGGTAAAGTC 
ACCCTCACAA CAGACTTATT AAGTACTAGT GACTTGAACA GTCCTGATGA AAACTTAGTT 
TTTACCATCA CCAGGGCTCC CATGAGAGGT CACTTAGAGT GCACAGATCA ACCTGGAGCA 
TCCATCACAT CGTTCTCTCA GCTCCAACTG GCTGGCAACA AAATCTACTA CATCCACACA 
GCTGACGATG AGGTCAAGAT GGACAGTTTT GAGTTTCAAG TCACTGATGG ACATAACCCT 
GTCTTCCGGA CATTCCGTAT CTCCATTAGC GATGTGGACA ACAAAAAGCC AGTGGTCACC 
ATCCATAACC TGGTTGTCAG TGAGGGTGAA AACAAGCTGA TCACTCCCTT TGAACTCACT 
GTCGAAGATC AAGATACTCC AGACAAACTC CTGAAGTTCA CTGTCACGCA GGTGCCTGTT 
CATGGTCAAC TCCTATTCAA CAATACCAGA CCTGCCATGG TTTTTACCAA GCAAGACCTG 
AACGAAAACT TAATCAGCTA CAGACATGAT GGCACTGAGT CAAATGAAGA CAGCTTCTCC 
TTCACAGTGA CTGATGGTTC CCATACAGAG TTCTATGTTT TCCCCGATAC AATATTTGAA 
ACAAGGAGAC CCCAAGTGAT GAAAATCCAA GTCTTACCTG TTGACGATAG CGTCCCCCAA 
ATTGCAGTGA ACAAAGGGGC CTCTACACTT CGCACTCTGG CTACTGGCCA CTTGGGATTC 
ATGATCACAA GCAAAATATT GAAAGTGGAG GACAGAGACA GCTTACGTTT TTCTCTCAGG 
TTTATTGTGA CGGAGGCCCC TCAACACGGG TATCTCCTCA ACCTGGACAG AGGCAACCAC 
AGTGTCTCTC AGTTTACACA AGCTGACATC GAAGACATGA AAATATGCTA CGTTTTAAAA 
GATGGGGCCA ATGCCACAAG TGATATGTTC CATTTTACAG TTGAAGATGG TGGCGGAAAC 
AAATTAACCA AACAGCATTT TCGTCTGAAT TGGGCATGGA TCTCGTTTGA AAAGGAATAT 
TACCTGATCA ATGAGGATTC CAAATTTCTA GATGTCGTTC TTAAACGCAG AGGTTACTTG 
GGAGAGACTT CATTTATAAG TATCGGCACA AGAGATGGGA CTGCAGAAAA AGACAAAGAC 
TTCAAGGGCA AAGCACAGAA ACAAGTGCAG TTCAACCCAG GCCAGACCAG GGCCACCTGG 
AGAGTGCGGA TCATGAGCGA TGGAGAACAC GAGCAGTCGG AGACCTTCCA GGTGGTTCTC 
TCAGAGCCTG TGCTGGCTGC TCTGGAATTC CCTGCAGTGA CCACGGTGGA GATTGTTGAC 
CCTGGAGATG AGTCGACTGT ATTTATTCCC CAGTCGGAAT ACTCCGTTGA AGAAGATGTG 
GGTGAGCTGT TCATTCCCAT CCGAAGAAGT GGAGACGTGA GCCAGGAATT GATGGTGGTC 
TGTTATACGC AACAAGGTAC GGCAGACGGA ACTGTGCCGA CGTCGGTGCT GTCCTACTCA 
GATTACATAT CCAGACCCGA AGACCACACC AGTGTCATCC ACTTTGACAG ACATGAGCGT 
GAAAAAACGT GTCGCATTGT TGTTATTGAC GACTCCCTGT ATGAAGAGGA GGAAACATTC 
CATGTCCTTC TGAGCGTGCC CATGGGCGGG AGGATTGGAT CAGAATACCC AAGGGCTCGG 
GTTACAATCG TCCCTGACAA AGATGATGAA CCCGTCTTCT ACTTCGGTGA TGTCGAGTAC 
TCCGTGGACG AGAGCGCCGG CTATGTAGAA GTGCGGGTGT GGAGGACGGG CACAGACCTG 
TCCAAGCCTT CCAGTGTCAC CGTGAGGTCT CGGAAAACAG ATCCCCCCTC TGCTGATGCT 
GGAACTGACT ATGTGGGAAT CAGCCGTAAT TTAGATTTTG CACCTGGAGT CAACATGCAG 
ACTGTTCGTG TTACCATCCT GGACGATCTC GGGCAACCAG TGCTGGAAGG AATTGAGAAA 
TTTGAACTGG TGCTTCGTAT GCCGATGAAC GCTGCCCTTG GCGAGCCCAG CAAAGCCACA 
GTGTCCATAA ATGATTCTGT CTCAGATTTG CCTAAGATGC AATTCAAAGA ACGGGTGTAT 
ATTGGCAATG AAAATGATGG GCAGATAGTC GCAATGATCC ACAGGAATGG AGACATTCAC 
TACAGGTCTT CAGTGAGATG CTACACACGC CAGGGGTCTG CACAGGTGAT GGCGGACTTT 
GAGGAACGTC CAAACACTGA TGGCTCCATC ATCACGTTCC TCCCTGGTGA GACTGAAAAG 
CCTTGTGTCC TCGAGCTGAT GGACGACAAA CTCTATGAGG AGGTAGAGGA GCTCCGCCTG 
GTTCTTGGCA CCCCACACAG CAACTCCCCC TTCGGGGCTG CGGTCGGGGA ACAAAATGAA 
ACTCTGATAA GGATCCAAGA TGATGCTGAT AAGGCCATCA TTAAATTTGG AGAAACCAAA 
TTTAGTGTCA GTGAACCCAA AGAACCAGGA CAGTCGGTGG TTATAAAAAT TCCAGTGATT 
CGCCAAGGAG ATACTTCGAA GGTTTCCATC GTGAGAGTCC ACACCAAGGA TGGATCAGCC 
ACCTCTGGAG AAGACTATCA CCCTGTGTCA GAAGAAATTG AGTTTAAGGA GGGAGAAACG 
CAGCATCTTG TTAACATTGA AGTAATCTTC GATGGGATCA GAGAAATGAG AGAGGCCTTC 
ACTGTTCACC TAAAACCCGA TGAGAACATG ATTGCAGAGA CTCAGGTCAC CAAAGCCATT 
GTGTATATAG AAGAAATGAA CAGCATGGCA GACGTCACTT TTCCTTCTGT CCCTCAAATT 
GTGTCGTTAC TGATGTATGA TGACACTTCC AGAGCTAAAG ATAGTGCTGG ACCTGTGTCT 
GGCTATCCTG TCGTCTGTAT CACGGCTTGC AACCCTAAAT ACTCAGACTA TGACAAAACA 
GGCTCCATTT GTGCAAGTGA GAACATCAAC GACACCTTGA CCCGGTACCG ATGGCTGACT 
AGCGCTCCCG CTGGCCCTGA TGGCGTGACC AGCCCCATGA GAGAAGTGGA CTTTGACACC 
TTCTTTACGT CATCCAAGAT GATCACCTTA GACTCCGTAT ACTTTCAGCC TGGCTCTCGG 
GTGCAGTGTG CGGCTCGTGC TGTGAATGCC GATGGCAATG AGGGCCTGGA GCTGATGAGC 
CCTGTCGTAA CCATCAGCAG AGAAGAAGGT CTTTGTCAGC CCCGAGTACC AGGGACTGTG 
GGAGCAGAAC CATTCTCCGC TAAACTGCGC TACACGGGCC CCGAGGACCC AGAGTACACA 
AACCTTATCA AGCTCACTGT CACGATGCCA CACGTAGACG GCATGCTCCC TGTGATCTCC 
ACTAAAGAGC TTTCCAACTT CGAGCTCACC CTCAGTCCTG ACGGCTCAAG AGTTGGAAAC 
CACCAGTGCT CCAATCTTCT GGACTATACT GAAGTGAAGA CCCACCATGG TTTCTTGACT 
GATGCTACAA AAAATCCAGA AATAATTGGA GAGACGCATC CTTACCAGTA CAGCTCGTCT 
ATCAGAGGTT CCAGTACCTT ACGCTTCTAT CGAAACCTGA ACCTGGAGGC CTGCTTATGG 
GAGTTTGTTA GCTACTATGA CATGTCAGAA CTTCTGACTG ACTGTGGAGG CACCATTGGG 
ACGGATGGAC AGGTCCTAAA TCTAGTACAG TCCTATGTGA CCCTTCGAGT GCCTCTGTAT 
GTTTCGTACG TGTTCCATTC TCCTGTTGGG GTAGGAGGCT GGCAACATTT TGACTTGAAG 
TCAGAGCTCC GACTGACTTT TGTGTATGAC ACTGCCATCT TGTGGAATGA TGGAATCGGC 
AGCCCACCAG AAGCGGAACT CCAAGGTTCT CTCTATCCCA CCAGCATGCG CATCGGCGAA 
GAGGGGCGCT TGGTCGTGAA CTTCAAGACG GAGGCTCAGT TCCACGGCTT GTTTGTACTC 
TCCCACCCGG CATCCTTCAC GAGCTCAATG ATCATGTCGG CTGACCATCC AGGCCTGACC 
TTTTCCCTGC GCCTCATAAG GAGCGAACCC ACCTACAACC AGCCAGTACA GCAATGGAGC 
TTTGTCTCTG ACTTTGCCGT GCGTGACTAT TCGGGGACCT ACACCGTAAA GCTGCTGCCA 
TGCACCATTC CATCGAATCA AGAGTACCGT CTGCCAGTCA CCTGCAACCC CAGAGAGCCT 
GTCACCTTTG ACCTTGACAT ACGGTTCCAA CAGGTCAGCG ACCCGGTAGC AGCTGAGTTT 
AGCTTGAACA CCCAAATGTA CCTACTCTCC AAGAAGAGTC TCTGGTTGTC TGATGGATCC 
ATGGGATTTG GGCAAGAGAG TGATGTCGCC TTTGCAGAAG GTGATATAAT TTATGGTCGA 
GTCATGGTAG ATCCTGTCCA GAATCTAGGT GATTCCTTTT ACTGCAGCAT TGAGAAGGTG 
TTCCTGTGCA CTGGAGCTGA TGGCTATGTT CCCAAATATA GTCCCACGAA TGCAGAATAT 
GGCTGCTTAG CTGATTCTCC TTCGCTCCTG TATAGATTTA AAATTGTGGA CAAAGCTCAG 
CCAGAGACAC AAGCCACCAG TTTTGGAAAT GTCCTGTTTA ATGCCAGACT AGCAGTGGAT 
GACCCTGAAG CTGTTCTCTT AGTGAATCAG CCTGGATCCG ATGGGTTTAA AGTCGACTCA 
ACAGCACTCT TTCAGGTGGC TCTGGGCCGA GAGTGGTACA TACACACCAT CTACACAGTA 
AGATCGAGAG ACAACGCCAA TCGAGGTATT GGCAAAAGAA GCGTGGAGTA CCAGCACCAC 
TCTTTGGTGA GTTCAGGGAA GCCCCGGGCA GGTACCAAAG GCCGGAAGAA GAGGGAGATG 
AGGAGCCCAC CCCCGCTGGC CTGGGAAATA GGTGCTGAAA ACAACCGGGG GACAAACATC 
CAGCACGTCG CCCTGGACCG CACCAGCAAG AAGCAGATCC CCCGAGGGAG AGTTCCTCCT 
GATGGCGTCC TCCCCCGGGA GCTCAACCCT CCCAGCTCTG AGGTCAGCCT GGCCACGGTG 
GTGGGAGGCA TCGCCGTGGG GTTACTCACC CTCTGCCTTG CCGCCATCAC GGTGACGATG 
TGCCAGAGCA AGAAGGGCGC CCGGAGAAAG GACGCCCTGA AGGGCTCGGG CAGCAGCAAG 
CCCATGATGC CCCCACAGAG CCACCACCAC GACAGCTCAG AGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003980389.1
CDS	258..9785
Translation	MHAARTPGIASHAGGVASPTSFQQIPTLPPRLVLLLLLLLLSLVSCVLAQPAAPGPALLSPDLAGAAGVPAEEAIVLANRGLRVPFGREVWLDPLQDLVLQVQPGDRCAVTVLDNDALAQRPGRLSPKRFPCDFGPREVRYSHLGARSPSRDRIRLQLRYDAPGGAAVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQAPGPASEGGAPEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVVELRSRGSPVGRPPLKREHFQVLVRIRGGAENTAPRPSFVAMMMMEVDQFVLTALTPDMLAAEDAESPPDLLIFNLTSPFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEAVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGLAGSGPQNLVVSDEDDLEAVQLEVVAGLRHGHLVLLGATDGNSAPKTFTVAELAAGQVVYHHDDRDGSLSDNLVLRMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETAPILPLALSATDIDSDDPLLLFVLESPSSTIGHLLLRQTHPPREEEELSRGPWRKQGAFYEQTVTEWRQRDITEGRLFYRHSGPHSPGPVMDQFTFRVQDNHDPPNQSGPHRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPTDTDENHSPAPLGTLVLTDNPSVVVAHFTQAQINHHKIAYRPPDRELGVAARVAQFQFQVEDQAGNVAPGTFTLYLQPVDNQPPEILNTGFTVQVKGHHILSETELRVNDVDTDVAHISFTLTRAPEHGHMRVSGQILHVGEVFRLEDIKQGRISYVHNGDGSLTDSCSLEVSDRHHVVPITLRVNVRPAGDEVPMLSLPAGTLGSYLDVLENGAAELTASVIKGTDGDTDDLMLTFLLEDPPLYGEILVNGVPAEQFTQRDILEGSVVYAHTSGEIGLLPKGDSFNLSLSDMSQEWVIGGSTIQGVTVWVTILPVDSQAPEISVGERFIVMEGDKSVITSTHISAEDIDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQSHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHFFPIIIIPTNDEQPEIFMREFMVMEGMSLVIDTPILNAADADIPPDDLTFTITQFPTRGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVERMVLIMVIPVDDETPRMTINNGLEIEIGETKIINNKILMATDLDSEDKSLVYIIRYGPGHGLLQRQKPAGAFENITVGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFYVSVGSVDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGASITSFSQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGHNPVFRTFRISISDVDNKKPVVTIHNLVVSEGENKLITPFELTVEDQDTPDKLLKFTVTQVPVHGQLLFNNTRPAMVFTKQDLNENLISYRHDGTESNEDSFSFTVTDGSHTEFYVFPDTIFETRRPQVMKIQVLPVDDSVPQIAVNKGASTLRTLATGHLGFMITSKILKVEDRDSLRFSLRFIVTEAPQHGYLLNLDRGNHSVSQFTQADIEDMKICYVLKDGANATSDMFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAEKDKDFKGKAQKQVQFNPGQTRATWRVRIMSDGEHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPQSEYSVEEDVGELFIPIRRSGDVSQELMVVCYTQQGTADGTVPTSVLSYSDYISRPEDHTSVIHFDRHEREKTCRIVVIDDSLYEEEETFHVLLSVPMGGRIGSEYPRARVTIVPDKDDEPVFYFGDVEYSVDESAGYVEVRVWRTGTDLSKPSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVTILDDLGQPVLEGIEKFELVLRMPMNAALGEPSKATVSINDSVSDLPKMQFKERVYIGNENDGQIVAMIHRNGDIHYRSSVRCYTRQGSAQVMADFEERPNTDGSIITFLPGETEKPCVLELMDDKLYEEVEELRLVLGTPHSNSPFGAAVGEQNETLIRIQDDADKAIIKFGETKFSVSEPKEPGQSVVIKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHLVNIEVIFDGIREMREAFTVHLKPDENMIAETQVTKAIVYIEEMNSMADVTFPSVPQIVSLLMYDDTSRAKDSAGPVSGYPVVCITACNPKYSDYDKTGSICASENINDTLTRYRWLTSAPAGPDGVTSPMREVDFDTFFTSSKMITLDSVYFQPGSRVQCAARAVNADGNEGLELMSPVVTISREEGLCQPRVPGTVGAEPFSAKLRYTGPEDPEYTNLIKLTVTMPHVDGMLPVISTKELSNFELTLSPDGSRVGNHQCSNLLDYTEVKTHHGFLTDATKNPEIIGETHPYQYSSSIRGSSTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNDGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSMIMSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTIPSNQEYRLPVTCNPREPVTFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYSPTNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNARLAVDDPEAVLLVNQPGSDGFKVDSTALFQVALGREWYIHTIYTVRSRDNANRGIGKRSVEYQHHSLVSSGKPRAGTKGRKKREMRSPPPLAWEIGAENNRGTNIQHVALDRTSKKQIPRGRVPPDGVLPRELNPPSSEVSLATVVGGIAVGLLTLCLAAITVTMCQSKKGARRKDALKGSGSSKPMMPPQSHHHDSSEV

Target ORF information:

RefSeq Version	XM_003980340.4
Organism	Felis catus(Domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003980340.4

ORF Insert Sequence:

ATGCACGCGG CCAGGACCCC CGGGATCGCC TCCCACGCCG GCGGGGTGGC CAGCCCCACC 
AGTTTTCAGC AGATCCCAAC GCTGCCGCCC CGCCTGGTGT TGCTGCTGCT GCTGCTTCTC 
CTGTCACTGG TAAGCTGCGT CCTGGCACAG CCCGCCGCCC CTGGCCCGGC GTTGCTGTCC 
CCGGATCTCG CGGGGGCTGC AGGGGTCCCC GCCGAGGAGG CCATCGTGCT GGCAAACCGT 
GGGCTCCGGG TGCCCTTTGG CCGTGAGGTC TGGCTGGATC CGCTGCAGGA CCTGGTGCTG 
CAGGTGCAGC CGGGGGACCG CTGCGCGGTG ACCGTGCTAG ACAACGACGC GCTGGCCCAG 
CGGCCCGGAC GCTTGAGTCC CAAGCGCTTC CCGTGCGACT TCGGCCCGAG AGAGGTGCGC 
TACTCGCACC TGGGCGCGCG CAGCCCGTCG CGGGATCGCA TTCGGCTGCA GTTGCGCTAC 
GACGCGCCGG GAGGGGCCGC AGTGCTACCC TTGGTGCTGG AGGTGGAGGT GGTCTTCACC 
CAGCTGGAGG TGGTGACTCG GAACCTGCCC CTAGTCGTGG AGGAGCTGCT GGGGACCAGC 
AACGCCCTGG ACGCGCGGAG CCTGGAGTTC GCCTACCAGC CGGAGACCGA GGAGTGTCGC 
GTAGGCATCC TGTCCGGCTT GAGCGCGCTG CCTCGCTATG GGGAGCTCCT CCACTACCCA 
CAGGCCCCGG GTCCAGCCAG CGAGGGGGGC GCCCCGGAGC CTCTCCTGAT GGACTGCAAA 
GCTTTCCAGG AGCTGGGGGT GCGCTACCGT CACACAGCCC CCAGTCGGTC ACCGAACAGG 
GACTGGGTGC CCATGGTGGT TGAGCTGCGC TCTCGGGGTT CGCCTGTGGG CAGACCCCCT 
TTGAAACGTG AGCACTTCCA GGTGCTGGTG AGAATCCGAG GAGGGGCGGA GAACACCGCA 
CCCAGGCCCA GTTTCGTGGC CATGATGATG ATGGAGGTGG ACCAGTTTGT ACTGACGGCT 
CTGACCCCAG ACATGCTGGC AGCCGAGGAT GCCGAGTCGC CCCCTGACCT GTTGATCTTC 
AACCTCACCT CTCCCTTCCA GCCTGGCCAG GGCTACCTAG TGAGTACTGA TGACCGCAGC 
CTGCCCCTCT CCTCCTTTAC TCAGAGGGAT CTGCGGCTCC TGAAGATTGC CTACCAGCCC 
CCCTCTGAGG ACTCTGACCA GGAGCGTCTC TTTGAACTGG AGTTGGAAGC AGTGGACCCG 
GAAGGAGCAG CCTCAGACCC TTTTGCCTTC ATGGTAGTGG TGAAGCCCAT GAACACACTG 
GCTCCCGTGG TCACCCGGAA CACCGGTCTC ATTCTCTATG AGGGCCAGTC TCGGCCCCTG 
ACGGGCCTTG CAGGCAGTGG ACCGCAGAAC TTGGTCGTCA GCGATGAGGA TGACCTAGAG 
GCAGTGCAGC TAGAGGTGGT GGCTGGGCTC CGCCACGGTC ACCTTGTCCT TCTGGGCGCC 
ACCGATGGCA ACTCTGCCCC TAAGACCTTC ACGGTGGCCG AGCTGGCCGC CGGCCAGGTG 
GTCTACCATC ACGATGACAG AGATGGCTCA CTGAGTGACA ACCTTGTACT TAGAATGGTG 
GACGGAGGAC GCAGGCACCA GGTGCAGTTC TTGTTCCCCA TCACCTTGGT GCCTGTGGAT 
GACCAGCCGC CAGTCCTCAA TGCTAACACG GGGCTGACGC TGGCGGAGGG TGAAACGGCG 
CCCATCCTTC CCCTGGCCCT GAGTGCAACG GACATCGACT CAGATGACCC TCTGCTGCTT 
TTTGTGCTGG AGTCGCCTTC TTCGACCATA GGGCATCTGC TTCTCCGCCA AACCCATCCT 
CCCCGCGAGG AAGAGGAGCT GAGCAGGGGC CCTTGGAGGA AACAGGGAGC GTTCTATGAG 
CAAACGGTGA CGGAGTGGAG GCAGCGGGAC ATAACAGAGG GGAGGCTGTT CTACAGGCAC 
TCCGGGCCCC ATAGTCCTGG GCCAGTGATG GACCAGTTCA CATTCCGGGT CCAGGATAAC 
CATGACCCCC CTAATCAGTC TGGACCACAC AGGTTTGTGA TTCGCATCCA TCCTGTGGAT 
CGCCTCCCTC CGGAGCTGGG CAGCGGCTGT CCCCTTCGGA TGGTGGTGCA AGAATCCCAG 
CTCACGCCGC TGAGGAAAAA GTGGCTGCGC TACACTGACC TGGACACAGA TGACCGAGAG 
CTGCGCTACA CCGTGACCCA GCCCCCCACG GACACAGATG AAAACCACTC ACCAGCCCCC 
CTGGGCACCT TGGTCCTGAC CGACAACCCC TCAGTCGTGG TGGCCCATTT TACCCAAGCC 
CAGATCAACC ACCATAAAAT TGCCTACAGA CCTCCGGATC GAGAACTGGG AGTGGCTGCC 
CGAGTGGCCC AGTTTCAATT CCAGGTGGAG GACCAAGCCG GGAATGTGGC TCCAGGCACC 
TTCACCCTTT ACTTGCAGCC CGTGGACAAC CAGCCGCCTG AGATCCTCAA CACGGGTTTC 
ACCGTTCAGG TGAAGGGCCA CCACATCCTG AGTGAGACTG AGTTACGTGT GAATGATGTA 
GACACCGATG TAGCCCACAT CTCTTTCACT CTCACACGGG CACCCGAACA TGGCCACATG 
AGAGTGTCTG GACAGATACT GCATGTGGGG GAGGTCTTCC GCTTGGAAGA TATAAAACAG 
GGTCGGATTT CCTATGTCCA TAATGGGGAC GGGTCCCTGA CTGACAGCTG CTCCTTGGAG 
GTCAGCGACA GACACCACGT GGTGCCCATC ACTCTCAGAG TGAATGTCCG GCCCGCGGGT 
GATGAGGTGC CCATGCTGAG CCTTCCTGCT GGTACTCTGG GGTCATATCT AGACGTTTTA 
GAAAATGGAG CTGCTGAACT CACTGCCAGT GTTATCAAGG GGACTGATGG GGACACTGAT 
GATTTGATGT TGACGTTCCT CTTGGAAGAC CCACCCTTGT ATGGAGAAAT CCTGGTCAAC 
GGAGTTCCGG CGGAGCAGTT TACCCAAAGG GACATCTTAG AGGGCTCTGT CGTCTATGCC 
CACACAAGCG GTGAGATAGG CCTGTTGCCC AAAGGGGATT CTTTTAACCT GAGTCTGTCA 
GATATGTCTC AAGAATGGGT AATAGGTGGC AGTACTATCC AAGGGGTTAC TGTGTGGGTG 
ACCATCCTCC CTGTCGATAG CCAAGCTCCA GAAATTTCTG TAGGTGAACG GTTTATAGTC 
ATGGAAGGTG ACAAAAGTGT TATAACCTCA ACACATATAA GTGCTGAAGA TATTGATTCC 
CTCAATGATG ACATCTTGTG CACTATAGTT ATTCAGCCTA CCTCTGGTTA TGTTGAAAAC 
ATTTCTCCAG CACCAGGCTC TGAGAAATCA AGAGCAGGAA TTGCCATAAG TGCCTTTACC 
CTGAAAGACC TCAGGCAGAG TCATATTAAC TATGTCCAGA GTGTCCACAA AGGGGTAGAA 
CCTGTGGAAG ACCGGTTTAT ATTTCGTTGC TCTGATGGCA TTAACTTTTC AGAGAGACAT 
TTTTTTCCCA TTATAATCAT TCCCACCAAT GATGAACAGC CAGAAATATT TATGAGAGAA 
TTTATGGTGA TGGAAGGCAT GAGTCTGGTG ATCGATACAC CCATCCTCAA TGCTGCTGAT 
GCTGACATTC CCCCGGATGA TCTAACTTTC ACCATTACCC AATTTCCCAC TCGTGGTCAC 
ATCATGAATC AGCTGATAAA TGGCACAGTG TTGGTTGAAA GCTTCACCTT GGACCAGATC 
ATAGAGAGTT CAAGCATTAT TTACGAGCAT GATGATTCTG AGACCCAGGA GGACAGTTTT 
GTGATTAAAC TAACAGACGG TAAGCACTCG GTGGAAAGGA TGGTCCTCAT TATGGTTATC 
CCAGTTGATG ATGAGACCCC CAGAATGACC ATCAATAATG GTCTAGAAAT AGAAATTGGG 
GAAACCAAAA TTATCAACAA CAAAATATTG ATGGCAACTG ATTTAGATTC TGAAGACAAA 
TCTTTGGTTT ATATTATTCG TTATGGGCCG GGACATGGCT TATTACAGAG ACAAAAACCC 
GCAGGTGCCT TTGAAAATAT CACAGTGGGC ATGAATTTTA CCCAGGATGA AGTGGACAGA 
AACTTAATTC AGTACGTCCA TTTTGGACAA GAGGGCATTC GGGACCTAAT TAAATTTGAT 
GTGACTGATG GAATAAATGC GCTTATAGAT CGCTACTTTT ATGTTTCTGT TGGGAGCGTT 
GACATTGTCT TCCCGGATGT GATAAGCAAG GGAGTATCCT TGAAAGAAGG TGGTAAAGTC 
ACCCTCACAA CAGACTTATT AAGTACTAGT GACTTGAACA GTCCTGATGA AAACTTAGTT 
TTTACCATCA CCAGGGCTCC CATGAGAGGT CACTTAGAGT GCACAGATCA ACCTGGAGCA 
TCCATCACAT CGTTCTCTCA GCTCCAACTG GCTGGCAACA AAATCTACTA CATCCACACA 
GCTGACGATG AGGTCAAGAT GGACAGTTTT GAGTTTCAAG TCACTGATGG ACATAACCCT 
GTCTTCCGGA CATTCCGTAT CTCCATTAGC GATGTGGACA ACAAAAAGCC AGTGGTCACC 
ATCCATAACC TGGTTGTCAG TGAGGGTGAA AACAAGCTGA TCACTCCCTT TGAACTCACT 
GTCGAAGATC AAGATACTCC AGACAAACTC CTGAAGTTCA CTGTCACGCA GGTGCCTGTT 
CATGGTCAAC TCCTATTCAA CAATACCAGA CCTGCCATGG TTTTTACCAA GCAAGACCTG 
AACGAAAACT TAATCAGCTA CAGACATGAT GGCACTGAGT CAAATGAAGA CAGCTTCTCC 
TTCACAGTGA CTGATGGTTC CCATACAGAG TTCTATGTTT TCCCCGATAC AATATTTGAA 
ACAAGGAGAC CCCAAGTGAT GAAAATCCAA GTCTTACCTG TTGACGATAG CGTCCCCCAA 
ATTGCAGTGA ACAAAGGGGC CTCTACACTT CGCACTCTGG CTACTGGCCA CTTGGGATTC 
ATGATCACAA GCAAAATATT GAAAGTGGAG GACAGAGACA GCTTACGTTT TTCTCTCAGG 
TTTATTGTGA CGGAGGCCCC TCAACACGGG TATCTCCTCA ACCTGGACAG AGGCAACCAC 
AGTGTCTCTC AGTTTACACA AGCTGACATC GAAGACATGA AAATATGCTA CGTTTTAAAA 
GATGGGGCCA ATGCCACAAG TGATATGTTC CATTTTACAG TTGAAGATGG TGGCGGAAAC 
AAATTAACCA AACAGCATTT TCGTCTGAAT TGGGCATGGA TCTCGTTTGA AAAGGAATAT 
TACCTGATCA ATGAGGATTC CAAATTTCTA GATGTCGTTC TTAAACGCAG AGGTTACTTG 
GGAGAGACTT CATTTATAAG TATCGGCACA AGAGATGGGA CTGCAGAAAA AGACAAAGAC 
TTCAAGGGCA AAGCACAGAA ACAAGTGCAG TTCAACCCAG GCCAGACCAG GGCCACCTGG 
AGAGTGCGGA TCATGAGCGA TGGAGAACAC GAGCAGTCGG AGACCTTCCA GGTGGTTCTC 
TCAGAGCCTG TGCTGGCTGC TCTGGAATTC CCTGCAGTGA CCACGGTGGA GATTGTTGAC 
CCTGGAGATG AGTCGACTGT ATTTATTCCC CAGTCGGAAT ACTCCGTTGA AGAAGATGTG 
GGTGAGCTGT TCATTCCCAT CCGAAGAAGT GGAGACGTGA GCCAGGAATT GATGGTGGTC 
TGTTATACGC AACAAGGTAC GGCAGACGGA ACTGTGCCGA CGTCGGTGCT GTCCTACTCA 
GATTACATAT CCAGACCCGA AGACCACACC AGTGTCATCC ACTTTGACAG ACATGAGCGT 
GAAAAAACGT GTCGCATTGT TGTTATTGAC GACTCCCTGT ATGAAGAGGA GGAAACATTC 
CATGTCCTTC TGAGCGTGCC CATGGGCGGG AGGATTGGAT CAGAATACCC AAGGGCTCGG 
GTTACAATCG TCCCTGACAA AGATGATGAA CCCGTCTTCT ACTTCGGTGA TGTCGAGTAC 
TCCGTGGACG AGAGCGCCGG CTATGTAGAA GTGCGGGTGT GGAGGACGGG CACAGACCTG 
TCCAAGCCTT CCAGTGTCAC CGTGAGGTCT CGGAAAACAG ATCCCCCCTC TGCTGATGCT 
GGAACTGACT ATGTGGGAAT CAGCCGTAAT TTAGATTTTG CACCTGGAGT CAACATGCAG 
ACTGTTCGTG TTACCATCCT GGACGATCTC GGGCAACCAG TGCTGGAAGG AATTGAGAAA 
TTTGAACTGG TGCTTCGTAT GCCGATGAAC GCTGCCCTTG GCGAGCCCAG CAAAGCCACA 
GTGTCCATAA ATGATTCTGT CTCAGATTTG CCTAAGATGC AATTCAAAGA ACGGGTGTAT 
ATTGGCAATG AAAATGATGG GCAGATAGTC GCAATGATCC ACAGGAATGG AGACATTCAC 
TACAGGTCTT CAGTGAGATG CTACACACGC CAGGGGTCTG CACAGGTGAT GGCGGACTTT 
GAGGAACGTC CAAACACTGA TGGCTCCATC ATCACGTTCC TCCCTGGTGA GACTGAAAAG 
CCTTGTGTCC TCGAGCTGAT GGACGACAAA CTCTATGAGG AGGTAGAGGA GCTCCGCCTG 
GTTCTTGGCA CCCCACACAG CAACTCCCCC TTCGGGGCTG CGGTCGGGGA ACAAAATGAA 
ACTCTGATAA GGATCCAAGA TGATGCTGAT AAGGCCATCA TTAAATTTGG AGAAACCAAA 
TTTAGTGTCA GTGAACCCAA AGAACCAGGA CAGTCGGTGG TTATAAAAAT TCCAGTGATT 
CGCCAAGGAG ATACTTCGAA GGTTTCCATC GTGAGAGTCC ACACCAAGGA TGGATCAGCC 
ACCTCTGGAG AAGACTATCA CCCTGTGTCA GAAGAAATTG AGTTTAAGGA GGGAGAAACG 
CAGCATCTTG TTAACATTGA AGTAATCTTC GATGGGATCA GAGAAATGAG AGAGGCCTTC 
ACTGTTCACC TAAAACCCGA TGAGAACATG ATTGCAGAGA CTCAGGTCAC CAAAGCCATT 
GTGTATATAG AAGAAATGAA CAGCATGGCA GACGTCACTT TTCCTTCTGT CCCTCAAATT 
GTGTCGTTAC TGATGTATGA TGACACTTCC AGAGCTAAAG ATAGTGCTGG ACCTGTGTCT 
GGCTATCCTG TCGTCTGTAT CACGGCTTGC AACCCTAAAT ACTCAGACTA TGACAAAACA 
GGCTCCATTT GTGCAAGTGA GAACATCAAC GACACCTTGA CCCGGTACCG ATGGCTGACT 
AGCGCTCCCG CTGGCCCTGA TGGCGTGACC AGCCCCATGA GAGAAGTGGA CTTTGACACC 
TTCTTTACGT CATCCAAGAT GATCACCTTA GACTCCGTAT ACTTTCAGCC TGGCTCTCGG 
GTGCAGTGTG CGGCTCGTGC TGTGAATGCC GATGGCAATG AGGGCCTGGA GCTGATGAGC 
CCTGTCGTAA CCATCAGCAG AGAAGAAGGT CTTTGTCAGC CCCGAGTACC AGGGACTGTG 
GGAGCAGAAC CATTCTCCGC TAAACTGCGC TACACGGGCC CCGAGGACCC AGAGTACACA 
AACCTTATCA AGCTCACTGT CACGATGCCA CACGTAGACG GCATGCTCCC TGTGATCTCC 
ACTAAAGAGC TTTCCAACTT CGAGCTCACC CTCAGTCCTG ACGGCTCAAG AGTTGGAAAC 
CACCAGTGCT CCAATCTTCT GGACTATACT GAAGTGAAGA CCCACCATGG TTTCTTGACT 
GATGCTACAA AAAATCCAGA AATAATTGGA GAGACGCATC CTTACCAGTA CAGCTCGTCT 
ATCAGAGGTT CCAGTACCTT ACGCTTCTAT CGAAACCTGA ACCTGGAGGC CTGCTTATGG 
GAGTTTGTTA GCTACTATGA CATGTCAGAA CTTCTGACTG ACTGTGGAGG CACCATTGGG 
ACGGATGGAC AGGTCCTAAA TCTAGTACAG TCCTATGTGA CCCTTCGAGT GCCTCTGTAT 
GTTTCGTACG TGTTCCATTC TCCTGTTGGG GTAGGAGGCT GGCAACATTT TGACTTGAAG 
TCAGAGCTCC GACTGACTTT TGTGTATGAC ACTGCCATCT TGTGGAATGA TGGAATCGGC 
AGCCCACCAG AAGCGGAACT CCAAGGTTCT CTCTATCCCA CCAGCATGCG CATCGGCGAA 
GAGGGGCGCT TGGTCGTGAA CTTCAAGACG GAGGCTCAGT TCCACGGCTT GTTTGTACTC 
TCCCACCCGG CATCCTTCAC GAGCTCAATG ATCATGTCGG CTGACCATCC AGGCCTGACC 
TTTTCCCTGC GCCTCATAAG GAGCGAACCC ACCTACAACC AGCCAGTACA GCAATGGAGC 
TTTGTCTCTG ACTTTGCCGT GCGTGACTAT TCGGGGACCT ACACCGTAAA GCTGCTGCCA 
TGCACCATTC CATCGAATCA AGAGTACCGT CTGCCAGTCA CCTGCAACCC CAGAGAGCCT 
GTCACCTTTG ACCTTGACAT ACGGTTCCAA CAGGTCAGCG ACCCGGTAGC AGCTGAGTTT 
AGCTTGAACA CCCAAATGTA CCTACTCTCC AAGAAGAGTC TCTGGTTGTC TGATGGATCC 
ATGGGATTTG GGCAAGAGAG TGATGTCGCC TTTGCAGAAG GTGATATAAT TTATGGTCGA 
GTCATGGTAG ATCCTGTCCA GAATCTAGGT GATTCCTTTT ACTGCAGCAT TGAGAAGGTG 
TTCCTGTGCA CTGGAGCTGA TGGCTATGTT CCCAAATATA GTCCCACGAA TGCAGAATAT 
GGCTGCTTAG CTGATTCTCC TTCGCTCCTG TATAGATTTA AAATTGTGGA CAAAGCTCAG 
CCAGAGACAC AAGCCACCAG TTTTGGAAAT GTCCTGTTTA ATGCCAGACT AGCAGTGGAT 
GACCCTGAAG CTGTTCTCTT AGTGAATCAG CCTGGATCCG ATGGGTTTAA AGTCGACTCA 
ACAGCACTCT TTCAGGTGGC TCTGGGCCGA GAGTGGTACA TACACACCAT CTACACAGTA 
AGATCGAGAG ACAACGCCAA TCGAGGTATT GGCAAAAGAA GCGTGGAGTA CCAGCACCAC 
TCTTTGGTGA GTTCAGGGAA GCCCCGGGCA GGTACCAAAG GCCGGAAGAA GAGGGAGATG 
AGGAGCCCAC CCCCGCTGGC CTGGGAAATA GGTGCTGAAA ACAACCGGGG GACAAACATC 
CAGCACGTCG CCCTGGACCG CACCAGCAAG AAGCAGATCC CCCGAGGGAG AGTTCCTCCT 
GATGGCGTCC TCCCCCGGGA GCTCAACCCT CCCAGCTCTG AGGTCAGCCT GGCCACGGTG 
GTGGGAGGCA TCGCCGTGGG GTTACTCACC CTCTGCCTTG CCGCCATCAC GGTGACGATG 
TGCCAGAGCA AGAAGGGCGC CCGGAGAAAG GACGCCCTGA AGGGCTCGGG CAGCAGCAAG 
CCCATGATGC CCCCACAGAG CCACCACCAC GACAGCTCAG AGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM2 ( XM_003980340.5 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_003980389.1 Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA.

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CloneID	OFb69998
Clone ID Related Accession (Same CDS sequence)	XM_003980340.5
Accession Version	XM_003980340.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	FRAS1-related extracellular matrix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018723.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_003980340.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGCGG CCAGGACCCC CGGGATCGCC TCCCACGCCG GCGGGGTGGC CAGCCCCACC 
AGTTTTCAGC AGATCCCAAC GCTGCCGCCC CGCCTGGTGT TGCTGCTGCT GCTGCTTCTC 
CTGTCACTGG TAAGCTGCGT CCTGGCACAG CCCGCCGCCC CTGGCCCGGC GTTGCTGTCC 
CCGGATCTCG CGGGGGCTGC AGGGGTCCCC GCCGAGGAGG CCATCGTGCT GGCAAACCGT 
GGGCTCCGGG TGCCCTTTGG CCGTGAGGTC TGGCTGGATC CGCTGCAGGA CCTGGTGCTG 
CAGGTGCAGC CGGGGGACCG CTGCGCGGTG ACCGTGCTAG ACAACGACGC GCTGGCCCAG 
CGGCCCGGAC GCTTGAGTCC CAAGCGCTTC CCGTGCGACT TCGGCCCGAG AGAGGTGCGC 
TACTCGCACC TGGGCGCGCG CAGCCCGTCG CGGGATCGCA TTCGGCTGCA GTTGCGCTAC 
GACGCGCCGG GAGGGGCCGC AGTGCTACCC TTGGTGCTGG AGGTGGAGGT GGTCTTCACC 
CAGCTGGAGG TGGTGACTCG GAACCTGCCC CTAGTCGTGG AGGAGCTGCT GGGGACCAGC 
AACGCCCTGG ACGCGCGGAG CCTGGAGTTC GCCTACCAGC CGGAGACCGA GGAGTGTCGC 
GTAGGCATCC TGTCCGGCTT GAGCGCGCTG CCTCGCTATG GGGAGCTCCT CCACTACCCA 
CAGGCCCCGG GTCCAGCCAG CGAGGGGGGC GCCCCGGAGC CTCTCCTGAT GGACTGCAAA 
GCTTTCCAGG AGCTGGGGGT GCGCTACCGT CACACAGCCC CCAGTCGGTC ACCGAACAGG 
GACTGGGTGC CCATGGTGGT TGAGCTGCGC TCTCGGGGTT CGCCTGTGGG CAGACCCCCT 
TTGAAACGTG AGCACTTCCA GGTGCTGGTG AGAATCCGAG GAGGGGCGGA GAACACCGCA 
CCCAGGCCCA GTTTCGTGGC CATGATGATG ATGGAGGTGG ACCAGTTTGT ACTGACGGCT 
CTGACCCCAG ACATGCTGGC AGCCGAGGAT GCCGAGTCGC CCCCTGACCT GTTGATCTTC 
AACCTCACCT CTCCCTTCCA GCCTGGCCAG GGCTACCTAG TGAGTACTGA TGACCGCAGC 
CTGCCCCTCT CCTCCTTTAC TCAGAGGGAT CTGCGGCTCC TGAAGATTGC CTACCAGCCC 
CCCTCTGAGG ACTCTGACCA GGAGCGTCTC TTTGAACTGG AGTTGGAAGC AGTGGACCCG 
GAAGGAGCAG CCTCAGACCC TTTTGCCTTC ATGGTAGTGG TGAAGCCCAT GAACACACTG 
GCTCCCGTGG TCACCCGGAA CACCGGTCTC ATTCTCTATG AGGGCCAGTC TCGGCCCCTG 
ACGGGCCTTG CAGGCAGTGG ACCGCAGAAC TTGGTCGTCA GCGATGAGGA TGACCTAGAG 
GCAGTGCAGC TAGAGGTGGT GGCTGGGCTC CGCCACGGTC ACCTTGTCCT TCTGGGCGCC 
ACCGATGGCA ACTCTGCCCC TAAGACCTTC ACGGTGGCCG AGCTGGCCGC CGGCCAGGTG 
GTCTACCATC ACGATGACAG AGATGGCTCA CTGAGTGACA ACCTTGTACT TAGAATGGTG 
GACGGAGGAC GCAGGCACCA GGTGCAGTTC TTGTTCCCCA TCACCTTGGT GCCTGTGGAT 
GACCAGCCGC CAGTCCTCAA TGCTAACACG GGGCTGACGC TGGCGGAGGG TGAAACGGCG 
CCCATCCTTC CCCTGGCCCT GAGTGCAACG GACATCGACT CAGATGACCC TCTGCTGCTT 
TTTGTGCTGG AGTCGCCTTC TTCGACCATA GGGCATCTGC TTCTCCGCCA AACCCATCCT 
CCCCGCGAGG AAGAGGAGCT GAGCAGGGGC CCTTGGAGGA AACAGGGAGC GTTCTATGAG 
CAAACGGTGA CGGAGTGGAG GCAGCGGGAC ATAACAGAGG GGAGGCTGTT CTACAGGCAC 
TCCGGGCCCC ATAGTCCTGG GCCAGTGATG GACCAGTTCA CATTCCGGGT CCAGGATAAC 
CATGACCCCC CTAATCAGTC TGGACCACAC AGGTTTGTGA TTCGCATCCA TCCTGTGGAT 
CGCCTCCCTC CGGAGCTGGG CAGCGGCTGT CCCCTTCGGA TGGTGGTGCA AGAATCCCAG 
CTCACGCCGC TGAGGAAAAA GTGGCTGCGC TACACTGACC TGGACACAGA TGACCGAGAG 
CTGCGCTACA CCGTGACCCA GCCCCCCACG GACACAGATG AAAACCACTC ACCAGCCCCC 
CTGGGCACCT TGGTCCTGAC CGACAACCCC TCAGTCGTGG TGGCCCATTT TACCCAAGCC 
CAGATCAACC ACCATAAAAT TGCCTACAGA CCTCCGGATC GAGAACTGGG AGTGGCTGCC 
CGAGTGGCCC AGTTTCAATT CCAGGTGGAG GACCAAGCCG GGAATGTGGC TCCAGGCACC 
TTCACCCTTT ACTTGCAGCC CGTGGACAAC CAGCCGCCTG AGATCCTCAA CACGGGTTTC 
ACCGTTCAGG TGAAGGGCCA CCACATCCTG AGTGAGACTG AGTTACGTGT GAATGATGTA 
GACACCGATG TAGCCCACAT CTCTTTCACT CTCACACGGG CACCCGAACA TGGCCACATG 
AGAGTGTCTG GACAGATACT GCATGTGGGG GAGGTCTTCC GCTTGGAAGA TATAAAACAG 
GGTCGGATTT CCTATGTCCA TAATGGGGAC GGGTCCCTGA CTGACAGCTG CTCCTTGGAG 
GTCAGCGACA GACACCACGT GGTGCCCATC ACTCTCAGAG TGAATGTCCG GCCCGCGGGT 
GATGAGGTGC CCATGCTGAG CCTTCCTGCT GGTACTCTGG GGTCATATCT AGACGTTTTA 
GAAAATGGAG CTGCTGAACT CACTGCCAGT GTTATCAAGG GGACTGATGG GGACACTGAT 
GATTTGATGT TGACGTTCCT CTTGGAAGAC CCACCCTTGT ATGGAGAAAT CCTGGTCAAC 
GGAGTTCCGG CGGAGCAGTT TACCCAAAGG GACATCTTAG AGGGCTCTGT CGTCTATGCC 
CACACAAGCG GTGAGATAGG CCTGTTGCCC AAAGGGGATT CTTTTAACCT GAGTCTGTCA 
GATATGTCTC AAGAATGGGT AATAGGTGGC AGTACTATCC AAGGGGTTAC TGTGTGGGTG 
ACCATCCTCC CTGTCGATAG CCAAGCTCCA GAAATTTCTG TAGGTGAACG GTTTATAGTC 
ATGGAAGGTG ACAAAAGTGT TATAACCTCA ACACATATAA GTGCTGAAGA TATTGATTCC 
CTCAATGATG ACATCTTGTG CACTATAGTT ATTCAGCCTA CCTCTGGTTA TGTTGAAAAC 
ATTTCTCCAG CACCAGGCTC TGAGAAATCA AGAGCAGGAA TTGCCATAAG TGCCTTTACC 
CTGAAAGACC TCAGGCAGAG TCATATTAAC TATGTCCAGA GTGTCCACAA AGGGGTAGAA 
CCTGTGGAAG ACCGGTTTAT ATTTCGTTGC TCTGATGGCA TTAACTTTTC AGAGAGACAT 
TTTTTTCCCA TTATAATCAT TCCCACCAAT GATGAACAGC CAGAAATATT TATGAGAGAA 
TTTATGGTGA TGGAAGGCAT GAGTCTGGTG ATCGATACAC CCATCCTCAA TGCTGCTGAT 
GCTGACATTC CCCCGGATGA TCTAACTTTC ACCATTACCC AATTTCCCAC TCGTGGTCAC 
ATCATGAATC AGCTGATAAA TGGCACAGTG TTGGTTGAAA GCTTCACCTT GGACCAGATC 
ATAGAGAGTT CAAGCATTAT TTACGAGCAT GATGATTCTG AGACCCAGGA GGACAGTTTT 
GTGATTAAAC TAACAGACGG TAAGCACTCG GTGGAAAGGA TGGTCCTCAT TATGGTTATC 
CCAGTTGATG ATGAGACCCC CAGAATGACC ATCAATAATG GTCTAGAAAT AGAAATTGGG 
GAAACCAAAA TTATCAACAA CAAAATATTG ATGGCAACTG ATTTAGATTC TGAAGACAAA 
TCTTTGGTTT ATATTATTCG TTATGGGCCG GGACATGGCT TATTACAGAG ACAAAAACCC 
GCAGGTGCCT TTGAAAATAT CACAGTGGGC ATGAATTTTA CCCAGGATGA AGTGGACAGA 
AACTTAATTC AGTACGTCCA TTTTGGACAA GAGGGCATTC GGGACCTAAT TAAATTTGAT 
GTGACTGATG GAATAAATGC GCTTATAGAT CGCTACTTTT ATGTTTCTGT TGGGAGCGTT 
GACATTGTCT TCCCGGATGT GATAAGCAAG GGAGTATCCT TGAAAGAAGG TGGTAAAGTC 
ACCCTCACAA CAGACTTATT AAGTACTAGT GACTTGAACA GTCCTGATGA AAACTTAGTT 
TTTACCATCA CCAGGGCTCC CATGAGAGGT CACTTAGAGT GCACAGATCA ACCTGGAGCA 
TCCATCACAT CGTTCTCTCA GCTCCAACTG GCTGGCAACA AAATCTACTA CATCCACACA 
GCTGACGATG AGGTCAAGAT GGACAGTTTT GAGTTTCAAG TCACTGATGG ACATAACCCT 
GTCTTCCGGA CATTCCGTAT CTCCATTAGC GATGTGGACA ACAAAAAGCC AGTGGTCACC 
ATCCATAACC TGGTTGTCAG TGAGGGTGAA AACAAGCTGA TCACTCCCTT TGAACTCACT 
GTCGAAGATC AAGATACTCC AGACAAACTC CTGAAGTTCA CTGTCACGCA GGTGCCTGTT 
CATGGTCAAC TCCTATTCAA CAATACCAGA CCTGCCATGG TTTTTACCAA GCAAGACCTG 
AACGAAAACT TAATCAGCTA CAGACATGAT GGCACTGAGT CAAATGAAGA CAGCTTCTCC 
TTCACAGTGA CTGATGGTTC CCATACAGAG TTCTATGTTT TCCCCGATAC AATATTTGAA 
ACAAGGAGAC CCCAAGTGAT GAAAATCCAA GTCTTACCTG TTGACGATAG CGTCCCCCAA 
ATTGCAGTGA ACAAAGGGGC CTCTACACTT CGCACTCTGG CTACTGGCCA CTTGGGATTC 
ATGATCACAA GCAAAATATT GAAAGTGGAG GACAGAGACA GCTTACGTTT TTCTCTCAGG 
TTTATTGTGA CGGAGGCCCC TCAACACGGG TATCTCCTCA ACCTGGACAG AGGCAACCAC 
AGTGTCTCTC AGTTTACACA AGCTGACATC GAAGACATGA AAATATGCTA CGTTTTAAAA 
GATGGGGCCA ATGCCACAAG TGATATGTTC CATTTTACAG TTGAAGATGG TGGTGGAAAC 
AAATTAACCA AACAGCATTT TCGTCTGAAT TGGGCATGGA TCTCGTTTGA AAAGGAATAT 
TACCTGATCA ATGAGGATTC CAAATTTCTA GATGTCGTTC TTAAACGCAG AGGTTACTTG 
GGAGAGACTT CATTTATAAG TATCGGCACA AGAGATGGGA CTGCAGAAAA AGACAAAGAC 
TTCAAGGGCA AAGCACAGAA ACAAGTGCAG TTCAACCCAG GCCAGACCAG GGCCACCTGG 
AGAGTGCGGA TCATGAGCGA TGGAGAACAC GAGCAGTCGG AGACCTTCCA GGTGGTTCTC 
TCAGAGCCTG TGCTGGCTGC TCTGGAATTC CCTGCAGTGA CCACGGTGGA GATTGTTGAC 
CCTGGAGATG AGTCGACTGT ATTTATTCCC CAGTCGGAAT ACTCCGTTGA AGAAGATGTG 
GGTGAGCTGT TCATTCCCAT CCGAAGAAGT GGAGACGTGA GCCAGGAATT GATGGTGGTC 
TGTTATACGC AACAAGGTAC GGCAGACGGA ACTGTGCCGA CGTCGGTGCT GTCCTACTCA 
GATTACATAT CCAGACCCGA AGACCACACC AGTGTCATCC ACTTTGACAG ACATGAGCGT 
GAAAAAACGT GTCGCATCGT TGTTATTGAC GACTCCCTGT ATGAAGAGGA GGAAACATTC 
CATGTCCTTC TGAGCGTGCC CATGGGCGGG AGGATTGGAT CAGAATACCC AAGGGCTCGG 
GTTACAATCG TCCCTGACAA AGATGATGAA CCCGTCTTCT ACTTCGGCGA TGTCGAGTAC 
TCCGTGGACG AGAGCGCCGG CTACGTGGAA GTGCGGGTGT GGAGGACGGG CACAGACCTG 
TCCAAGCCTT CCAGTGTCAC CGTGAGGTCT CGGAAAACAG ATCCCCCCTC TGCTGATGCT 
GGAACTGACT ATGTGGGAAT CAGCCGTAAT TTAGATTTTG CACCTGGAGT CAACATGCAG 
ACTGTTCGTG TTACCATCCT GGACGATCTC GGGCAACCAG TGCTGGAAGG AATTGAGAAA 
TTTGAACTGG TGCTTCGTAT GCCGATGAAC GCTGCCCTTG GCGAGCCCAG CAAAGCCACA 
GTGTCCATAA ATGATTCTGT CTCAGATTTG CCTAAGATGC AATTCAAAGA ACGGGTGTAT 
ATTGGCAATG AAAATGATGG GCAGATAGTC GCAATGATCC ACAGGAATGG AGACATTCAC 
TACAGGTCTT CAGTGAGATG CTACACACGC CAGGGGTCTG CACAGGTGAT GGCGGACTTT 
GAGGAACGTC CAAACACTGA TGGCTCCATC ATCACGTTCC TCCCTGGTGA GACTGAAAAG 
CCTTGTGTCC TCGAGCTGAT GGACGACAAA CTCTATGAGG AGGTAGAGGA GCTCCGCCTG 
GTTCTTGGCA CCCCACACAG CAACTCCCCC TTCGGGGCTG CGGTCGGGGA ACAAAATGAA 
ACTCTGATAA GGATCCAAGA TGATGCTGAT AAGGCCATCA TTAAATTTGG AGAAACCAAA 
TTTAGTGTCA GTGAACCCAA AGAACCAGGA CAGTCGGTGG TTATAAAAAT TCCAGTGATT 
CGCCAAGGAG ATACTTCGAA GGTTTCCATC GTGAGAGTCC ACACCAAGGA TGGATCAGCC 
ACCTCTGGAG AAGACTATCA CCCTGTGTCA GAAGAAATTG AGTTTAAGGA GGGAGAAACG 
CAGCATCTTG TTAACATTGA AGTAATCTTC GATGGGATCA GAGAAATGAG AGAGGCCTTC 
ACTGTTCACC TAAAACCCGA TGAGAACATG ATTGCAGAGA CTCAGGTCAC CAAAGCCATT 
GTGTATATAG AAGAAATGAA CAGCATGGCA GACGTCACTT TTCCTTCTGT CCCTCAAATT 
GTGTCATTAC TGATGTATGA TGACACTTCC AGAGCTAAAG ATAGTGCTGG ACCTGTGTCT 
GGCTATCCTG TCGTCTGTAT CACGGCTTGC AACCCTAAAT ACTCAGACTA TGACAAAACA 
GGCTCCATTT GTGCAAGTGA GAACATCAAC GACACCTTGA CCCGGTACCG ATGGCTGACT 
AGCGCTCCCG CTGGCCCTGA TGGCGTGACC AGCCCCATGA GAGAAGTGGA CTTTGACACC 
TTCTTTACGT CATCCAAGAT GATCACCTTA GACTCCGTAT ACTTTCAGCC TGGCTCTCGG 
GTGCAGTGTG CGGCTCGTGC TGTGAATGCC GATGGCAATG AGGGCCTGGA GCTGATGAGC 
CCTGTCGTAA CCATCAGCAG AGAAGAAGGT CTTTGTCAGC CCCGAGTACC AGGGACTGTG 
GGAGCAGAAC CATTCTCCGC TAAACTGCGC TACACGGGCC CCGAGGACCC AGAGTACACA 
AACCTTATCA AGCTCACTGT CACGATGCCA CACGTAGACG GCATGCTCCC TGTGATCTCC 
ACTAAAGAGC TTTCCAACTT CGAGCTCACC CTCAGTCCTG ACGGCTCAAG AGTTGGAAAC 
CACCAGTGCT CCAATCTTCT GGACTATACT GAAGTGAAGA CCCACCATGG TTTCTTGACT 
GATGCTACAA AAAATCCAGA AATAATTGGA GAGACGCATC CTTACCAGTA CAGCTCGTCT 
ATCAGAGGTT CCAGTACCTT ACGCTTCTAT CGAAACCTGA ACCTGGAGGC CTGCTTATGG 
GAGTTTGTTA GCTACTATGA CATGTCAGAA CTTCTGACTG ACTGTGGAGG CACCATCGGG 
ACGGATGGAC AGGTCCTAAA TCTAGTACAG TCCTATGTGA CCCTTCGAGT GCCTCTGTAT 
GTTTCGTACG TGTTCCATTC TCCTGTTGGG GTAGGAGGCT GGCAACATTT TGACTTGAAG 
TCAGAGCTCC GACTGACTTT TGTGTATGAC ACTGCCATCT TGTGGAATGA TGGAATCGGC 
AGCCCACCAG AAGCGGAACT CCAAGGTTCT CTCTATCCCA CCAGCATGCG CATCGGCGAA 
GAGGGGCGCT TGGTCGTGAA CTTCAAGACG GAGGCTCAGT TCCACGGCTT GTTTGTACTC 
TCCCACCCGG CATCCTTCAC GAGCTCAATG ATCATGTCGG CTGACCATCC AGGCCTGACC 
TTTTCCCTGC GCCTCATAAG GAGCGAACCC ACCTACAACC AGCCAGTACA GCAATGGAGC 
TTTGTCTCTG ACTTTGCCGT GCGTGACTAT TCGGGGACCT ACACCGTAAA GCTGCTGCCA 
TGCACCATTC CATCGAATCA AGAGTACCGT CTGCCAGTCA CCTGCAACCC CAGAGAGCCT 
GTCACCTTTG ACCTTGACAT ACGGTTCCAA CAGGTCAGCG ACCCGGTAGC AGCTGAGTTT 
AGCTTGAACA CCCAAATGTA CCTACTCTCC AAGAAGAGTC TCTGGTTGTC TGATGGATCC 
ATGGGATTTG GGCAAGAGAG TGATGTCGCC TTTGCAGAAG GTGATATAAT TTATGGTCGA 
GTCATGGTAG ATCCTGTCCA GAATCTAGGT GATTCCTTTT ACTGCAGCAT TGAGAAGGTG 
TTCCTGTGCA CTGGAGCTGA TGGCTATGTT CCCAAATATA GTCCCACGAA TGCAGAATAT 
GGCTGCTTAG CTGATTCTCC TTCGCTCCTG TATAGATTTA AAATTGTGGA CAAAGCTCAG 
CCAGAGACAC AAGCCACCAG TTTTGGAAAT GTCCTGTTTA ATGCCAGACT AGCAGTGGAT 
GACCCTGAAG CTGTTCTCTT AGTGAATCAG CCTGGATCCG ATGGGTTTAA AGTCGACTCA 
ACAGCACTCT TTCAGGTGGC TCTGGGCCGA GAGTGGTACA TACACACCAT CTACACAGTA 
AGATCGAGAG ACAACGCCAA TCGAGGTATT GGCAAAAGAA GCGTGGAGTA CCAGCACCAC 
TCTTTGGTGA GTTCAGGGAA GCCCCGGGCA GGTACCAAAG GCCGGAAGAA GAGGGAGATG 
AGGAGCCCAC CCCCGCTGGC CTGGGAAATA GGTGCTGAAA ACAACCGGGG GACAAACATC 
CAGCACGTCG CCCTGGACCG CACCAGCAAG AAGCAGATCC CCCGAGGGAG AGTTCCTCCT 
GATGGCGTCC TCCCCCGGGA GCTCAACCCT CCCAGCTCTG AGGTCAGCCT GGCCACGGTG 
GTGGGAGGCA TCGCCGTGGG GTTACTCACC CTCTGCCTTG CCGCCATCAC GGTGACGATG 
TGCCAGAGCA AGAAGGGCGC CCGGAGAAAG GACGCCCTGA AGGGCTCGGG CAGCAGCAAG 
CCCATGATGC CCCCACAGAG CCACCACCAC GACAGCTCAG AGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003980389.1
CDS	258..9785
Translation	MHAARTPGIASHAGGVASPTSFQQIPTLPPRLVLLLLLLLLSLVSCVLAQPAAPGPALLSPDLAGAAGVPAEEAIVLANRGLRVPFGREVWLDPLQDLVLQVQPGDRCAVTVLDNDALAQRPGRLSPKRFPCDFGPREVRYSHLGARSPSRDRIRLQLRYDAPGGAAVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQAPGPASEGGAPEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVVELRSRGSPVGRPPLKREHFQVLVRIRGGAENTAPRPSFVAMMMMEVDQFVLTALTPDMLAAEDAESPPDLLIFNLTSPFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEAVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGLAGSGPQNLVVSDEDDLEAVQLEVVAGLRHGHLVLLGATDGNSAPKTFTVAELAAGQVVYHHDDRDGSLSDNLVLRMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETAPILPLALSATDIDSDDPLLLFVLESPSSTIGHLLLRQTHPPREEEELSRGPWRKQGAFYEQTVTEWRQRDITEGRLFYRHSGPHSPGPVMDQFTFRVQDNHDPPNQSGPHRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPTDTDENHSPAPLGTLVLTDNPSVVVAHFTQAQINHHKIAYRPPDRELGVAARVAQFQFQVEDQAGNVAPGTFTLYLQPVDNQPPEILNTGFTVQVKGHHILSETELRVNDVDTDVAHISFTLTRAPEHGHMRVSGQILHVGEVFRLEDIKQGRISYVHNGDGSLTDSCSLEVSDRHHVVPITLRVNVRPAGDEVPMLSLPAGTLGSYLDVLENGAAELTASVIKGTDGDTDDLMLTFLLEDPPLYGEILVNGVPAEQFTQRDILEGSVVYAHTSGEIGLLPKGDSFNLSLSDMSQEWVIGGSTIQGVTVWVTILPVDSQAPEISVGERFIVMEGDKSVITSTHISAEDIDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQSHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHFFPIIIIPTNDEQPEIFMREFMVMEGMSLVIDTPILNAADADIPPDDLTFTITQFPTRGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVERMVLIMVIPVDDETPRMTINNGLEIEIGETKIINNKILMATDLDSEDKSLVYIIRYGPGHGLLQRQKPAGAFENITVGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFYVSVGSVDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGASITSFSQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGHNPVFRTFRISISDVDNKKPVVTIHNLVVSEGENKLITPFELTVEDQDTPDKLLKFTVTQVPVHGQLLFNNTRPAMVFTKQDLNENLISYRHDGTESNEDSFSFTVTDGSHTEFYVFPDTIFETRRPQVMKIQVLPVDDSVPQIAVNKGASTLRTLATGHLGFMITSKILKVEDRDSLRFSLRFIVTEAPQHGYLLNLDRGNHSVSQFTQADIEDMKICYVLKDGANATSDMFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAEKDKDFKGKAQKQVQFNPGQTRATWRVRIMSDGEHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPQSEYSVEEDVGELFIPIRRSGDVSQELMVVCYTQQGTADGTVPTSVLSYSDYISRPEDHTSVIHFDRHEREKTCRIVVIDDSLYEEEETFHVLLSVPMGGRIGSEYPRARVTIVPDKDDEPVFYFGDVEYSVDESAGYVEVRVWRTGTDLSKPSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVTILDDLGQPVLEGIEKFELVLRMPMNAALGEPSKATVSINDSVSDLPKMQFKERVYIGNENDGQIVAMIHRNGDIHYRSSVRCYTRQGSAQVMADFEERPNTDGSIITFLPGETEKPCVLELMDDKLYEEVEELRLVLGTPHSNSPFGAAVGEQNETLIRIQDDADKAIIKFGETKFSVSEPKEPGQSVVIKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHLVNIEVIFDGIREMREAFTVHLKPDENMIAETQVTKAIVYIEEMNSMADVTFPSVPQIVSLLMYDDTSRAKDSAGPVSGYPVVCITACNPKYSDYDKTGSICASENINDTLTRYRWLTSAPAGPDGVTSPMREVDFDTFFTSSKMITLDSVYFQPGSRVQCAARAVNADGNEGLELMSPVVTISREEGLCQPRVPGTVGAEPFSAKLRYTGPEDPEYTNLIKLTVTMPHVDGMLPVISTKELSNFELTLSPDGSRVGNHQCSNLLDYTEVKTHHGFLTDATKNPEIIGETHPYQYSSSIRGSSTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNDGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSMIMSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTIPSNQEYRLPVTCNPREPVTFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYSPTNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNARLAVDDPEAVLLVNQPGSDGFKVDSTALFQVALGREWYIHTIYTVRSRDNANRGIGKRSVEYQHHSLVSSGKPRAGTKGRKKREMRSPPPLAWEIGAENNRGTNIQHVALDRTSKKQIPRGRVPPDGVLPRELNPPSSEVSLATVVGGIAVGLLTLCLAAITVTMCQSKKGARRKDALKGSGSSKPMMPPQSHHHDSSEV

Target ORF information:

RefSeq Version	XM_003980340.5
Organism	Felis catus(domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003980340.5

ORF Insert Sequence:

ATGCACGCGG CCAGGACCCC CGGGATCGCC TCCCACGCCG GCGGGGTGGC CAGCCCCACC 
AGTTTTCAGC AGATCCCAAC GCTGCCGCCC CGCCTGGTGT TGCTGCTGCT GCTGCTTCTC 
CTGTCACTGG TAAGCTGCGT CCTGGCACAG CCCGCCGCCC CTGGCCCGGC GTTGCTGTCC 
CCGGATCTCG CGGGGGCTGC AGGGGTCCCC GCCGAGGAGG CCATCGTGCT GGCAAACCGT 
GGGCTCCGGG TGCCCTTTGG CCGTGAGGTC TGGCTGGATC CGCTGCAGGA CCTGGTGCTG 
CAGGTGCAGC CGGGGGACCG CTGCGCGGTG ACCGTGCTAG ACAACGACGC GCTGGCCCAG 
CGGCCCGGAC GCTTGAGTCC CAAGCGCTTC CCGTGCGACT TCGGCCCGAG AGAGGTGCGC 
TACTCGCACC TGGGCGCGCG CAGCCCGTCG CGGGATCGCA TTCGGCTGCA GTTGCGCTAC 
GACGCGCCGG GAGGGGCCGC AGTGCTACCC TTGGTGCTGG AGGTGGAGGT GGTCTTCACC 
CAGCTGGAGG TGGTGACTCG GAACCTGCCC CTAGTCGTGG AGGAGCTGCT GGGGACCAGC 
AACGCCCTGG ACGCGCGGAG CCTGGAGTTC GCCTACCAGC CGGAGACCGA GGAGTGTCGC 
GTAGGCATCC TGTCCGGCTT GAGCGCGCTG CCTCGCTATG GGGAGCTCCT CCACTACCCA 
CAGGCCCCGG GTCCAGCCAG CGAGGGGGGC GCCCCGGAGC CTCTCCTGAT GGACTGCAAA 
GCTTTCCAGG AGCTGGGGGT GCGCTACCGT CACACAGCCC CCAGTCGGTC ACCGAACAGG 
GACTGGGTGC CCATGGTGGT TGAGCTGCGC TCTCGGGGTT CGCCTGTGGG CAGACCCCCT 
TTGAAACGTG AGCACTTCCA GGTGCTGGTG AGAATCCGAG GAGGGGCGGA GAACACCGCA 
CCCAGGCCCA GTTTCGTGGC CATGATGATG ATGGAGGTGG ACCAGTTTGT ACTGACGGCT 
CTGACCCCAG ACATGCTGGC AGCCGAGGAT GCCGAGTCGC CCCCTGACCT GTTGATCTTC 
AACCTCACCT CTCCCTTCCA GCCTGGCCAG GGCTACCTAG TGAGTACTGA TGACCGCAGC 
CTGCCCCTCT CCTCCTTTAC TCAGAGGGAT CTGCGGCTCC TGAAGATTGC CTACCAGCCC 
CCCTCTGAGG ACTCTGACCA GGAGCGTCTC TTTGAACTGG AGTTGGAAGC AGTGGACCCG 
GAAGGAGCAG CCTCAGACCC TTTTGCCTTC ATGGTAGTGG TGAAGCCCAT GAACACACTG 
GCTCCCGTGG TCACCCGGAA CACCGGTCTC ATTCTCTATG AGGGCCAGTC TCGGCCCCTG 
ACGGGCCTTG CAGGCAGTGG ACCGCAGAAC TTGGTCGTCA GCGATGAGGA TGACCTAGAG 
GCAGTGCAGC TAGAGGTGGT GGCTGGGCTC CGCCACGGTC ACCTTGTCCT TCTGGGCGCC 
ACCGATGGCA ACTCTGCCCC TAAGACCTTC ACGGTGGCCG AGCTGGCCGC CGGCCAGGTG 
GTCTACCATC ACGATGACAG AGATGGCTCA CTGAGTGACA ACCTTGTACT TAGAATGGTG 
GACGGAGGAC GCAGGCACCA GGTGCAGTTC TTGTTCCCCA TCACCTTGGT GCCTGTGGAT 
GACCAGCCGC CAGTCCTCAA TGCTAACACG GGGCTGACGC TGGCGGAGGG TGAAACGGCG 
CCCATCCTTC CCCTGGCCCT GAGTGCAACG GACATCGACT CAGATGACCC TCTGCTGCTT 
TTTGTGCTGG AGTCGCCTTC TTCGACCATA GGGCATCTGC TTCTCCGCCA AACCCATCCT 
CCCCGCGAGG AAGAGGAGCT GAGCAGGGGC CCTTGGAGGA AACAGGGAGC GTTCTATGAG 
CAAACGGTGA CGGAGTGGAG GCAGCGGGAC ATAACAGAGG GGAGGCTGTT CTACAGGCAC 
TCCGGGCCCC ATAGTCCTGG GCCAGTGATG GACCAGTTCA CATTCCGGGT CCAGGATAAC 
CATGACCCCC CTAATCAGTC TGGACCACAC AGGTTTGTGA TTCGCATCCA TCCTGTGGAT 
CGCCTCCCTC CGGAGCTGGG CAGCGGCTGT CCCCTTCGGA TGGTGGTGCA AGAATCCCAG 
CTCACGCCGC TGAGGAAAAA GTGGCTGCGC TACACTGACC TGGACACAGA TGACCGAGAG 
CTGCGCTACA CCGTGACCCA GCCCCCCACG GACACAGATG AAAACCACTC ACCAGCCCCC 
CTGGGCACCT TGGTCCTGAC CGACAACCCC TCAGTCGTGG TGGCCCATTT TACCCAAGCC 
CAGATCAACC ACCATAAAAT TGCCTACAGA CCTCCGGATC GAGAACTGGG AGTGGCTGCC 
CGAGTGGCCC AGTTTCAATT CCAGGTGGAG GACCAAGCCG GGAATGTGGC TCCAGGCACC 
TTCACCCTTT ACTTGCAGCC CGTGGACAAC CAGCCGCCTG AGATCCTCAA CACGGGTTTC 
ACCGTTCAGG TGAAGGGCCA CCACATCCTG AGTGAGACTG AGTTACGTGT GAATGATGTA 
GACACCGATG TAGCCCACAT CTCTTTCACT CTCACACGGG CACCCGAACA TGGCCACATG 
AGAGTGTCTG GACAGATACT GCATGTGGGG GAGGTCTTCC GCTTGGAAGA TATAAAACAG 
GGTCGGATTT CCTATGTCCA TAATGGGGAC GGGTCCCTGA CTGACAGCTG CTCCTTGGAG 
GTCAGCGACA GACACCACGT GGTGCCCATC ACTCTCAGAG TGAATGTCCG GCCCGCGGGT 
GATGAGGTGC CCATGCTGAG CCTTCCTGCT GGTACTCTGG GGTCATATCT AGACGTTTTA 
GAAAATGGAG CTGCTGAACT CACTGCCAGT GTTATCAAGG GGACTGATGG GGACACTGAT 
GATTTGATGT TGACGTTCCT CTTGGAAGAC CCACCCTTGT ATGGAGAAAT CCTGGTCAAC 
GGAGTTCCGG CGGAGCAGTT TACCCAAAGG GACATCTTAG AGGGCTCTGT CGTCTATGCC 
CACACAAGCG GTGAGATAGG CCTGTTGCCC AAAGGGGATT CTTTTAACCT GAGTCTGTCA 
GATATGTCTC AAGAATGGGT AATAGGTGGC AGTACTATCC AAGGGGTTAC TGTGTGGGTG 
ACCATCCTCC CTGTCGATAG CCAAGCTCCA GAAATTTCTG TAGGTGAACG GTTTATAGTC 
ATGGAAGGTG ACAAAAGTGT TATAACCTCA ACACATATAA GTGCTGAAGA TATTGATTCC 
CTCAATGATG ACATCTTGTG CACTATAGTT ATTCAGCCTA CCTCTGGTTA TGTTGAAAAC 
ATTTCTCCAG CACCAGGCTC TGAGAAATCA AGAGCAGGAA TTGCCATAAG TGCCTTTACC 
CTGAAAGACC TCAGGCAGAG TCATATTAAC TATGTCCAGA GTGTCCACAA AGGGGTAGAA 
CCTGTGGAAG ACCGGTTTAT ATTTCGTTGC TCTGATGGCA TTAACTTTTC AGAGAGACAT 
TTTTTTCCCA TTATAATCAT TCCCACCAAT GATGAACAGC CAGAAATATT TATGAGAGAA 
TTTATGGTGA TGGAAGGCAT GAGTCTGGTG ATCGATACAC CCATCCTCAA TGCTGCTGAT 
GCTGACATTC CCCCGGATGA TCTAACTTTC ACCATTACCC AATTTCCCAC TCGTGGTCAC 
ATCATGAATC AGCTGATAAA TGGCACAGTG TTGGTTGAAA GCTTCACCTT GGACCAGATC 
ATAGAGAGTT CAAGCATTAT TTACGAGCAT GATGATTCTG AGACCCAGGA GGACAGTTTT 
GTGATTAAAC TAACAGACGG TAAGCACTCG GTGGAAAGGA TGGTCCTCAT TATGGTTATC 
CCAGTTGATG ATGAGACCCC CAGAATGACC ATCAATAATG GTCTAGAAAT AGAAATTGGG 
GAAACCAAAA TTATCAACAA CAAAATATTG ATGGCAACTG ATTTAGATTC TGAAGACAAA 
TCTTTGGTTT ATATTATTCG TTATGGGCCG GGACATGGCT TATTACAGAG ACAAAAACCC 
GCAGGTGCCT TTGAAAATAT CACAGTGGGC ATGAATTTTA CCCAGGATGA AGTGGACAGA 
AACTTAATTC AGTACGTCCA TTTTGGACAA GAGGGCATTC GGGACCTAAT TAAATTTGAT 
GTGACTGATG GAATAAATGC GCTTATAGAT CGCTACTTTT ATGTTTCTGT TGGGAGCGTT 
GACATTGTCT TCCCGGATGT GATAAGCAAG GGAGTATCCT TGAAAGAAGG TGGTAAAGTC 
ACCCTCACAA CAGACTTATT AAGTACTAGT GACTTGAACA GTCCTGATGA AAACTTAGTT 
TTTACCATCA CCAGGGCTCC CATGAGAGGT CACTTAGAGT GCACAGATCA ACCTGGAGCA 
TCCATCACAT CGTTCTCTCA GCTCCAACTG GCTGGCAACA AAATCTACTA CATCCACACA 
GCTGACGATG AGGTCAAGAT GGACAGTTTT GAGTTTCAAG TCACTGATGG ACATAACCCT 
GTCTTCCGGA CATTCCGTAT CTCCATTAGC GATGTGGACA ACAAAAAGCC AGTGGTCACC 
ATCCATAACC TGGTTGTCAG TGAGGGTGAA AACAAGCTGA TCACTCCCTT TGAACTCACT 
GTCGAAGATC AAGATACTCC AGACAAACTC CTGAAGTTCA CTGTCACGCA GGTGCCTGTT 
CATGGTCAAC TCCTATTCAA CAATACCAGA CCTGCCATGG TTTTTACCAA GCAAGACCTG 
AACGAAAACT TAATCAGCTA CAGACATGAT GGCACTGAGT CAAATGAAGA CAGCTTCTCC 
TTCACAGTGA CTGATGGTTC CCATACAGAG TTCTATGTTT TCCCCGATAC AATATTTGAA 
ACAAGGAGAC CCCAAGTGAT GAAAATCCAA GTCTTACCTG TTGACGATAG CGTCCCCCAA 
ATTGCAGTGA ACAAAGGGGC CTCTACACTT CGCACTCTGG CTACTGGCCA CTTGGGATTC 
ATGATCACAA GCAAAATATT GAAAGTGGAG GACAGAGACA GCTTACGTTT TTCTCTCAGG 
TTTATTGTGA CGGAGGCCCC TCAACACGGG TATCTCCTCA ACCTGGACAG AGGCAACCAC 
AGTGTCTCTC AGTTTACACA AGCTGACATC GAAGACATGA AAATATGCTA CGTTTTAAAA 
GATGGGGCCA ATGCCACAAG TGATATGTTC CATTTTACAG TTGAAGATGG TGGTGGAAAC 
AAATTAACCA AACAGCATTT TCGTCTGAAT TGGGCATGGA TCTCGTTTGA AAAGGAATAT 
TACCTGATCA ATGAGGATTC CAAATTTCTA GATGTCGTTC TTAAACGCAG AGGTTACTTG 
GGAGAGACTT CATTTATAAG TATCGGCACA AGAGATGGGA CTGCAGAAAA AGACAAAGAC 
TTCAAGGGCA AAGCACAGAA ACAAGTGCAG TTCAACCCAG GCCAGACCAG GGCCACCTGG 
AGAGTGCGGA TCATGAGCGA TGGAGAACAC GAGCAGTCGG AGACCTTCCA GGTGGTTCTC 
TCAGAGCCTG TGCTGGCTGC TCTGGAATTC CCTGCAGTGA CCACGGTGGA GATTGTTGAC 
CCTGGAGATG AGTCGACTGT ATTTATTCCC CAGTCGGAAT ACTCCGTTGA AGAAGATGTG 
GGTGAGCTGT TCATTCCCAT CCGAAGAAGT GGAGACGTGA GCCAGGAATT GATGGTGGTC 
TGTTATACGC AACAAGGTAC GGCAGACGGA ACTGTGCCGA CGTCGGTGCT GTCCTACTCA 
GATTACATAT CCAGACCCGA AGACCACACC AGTGTCATCC ACTTTGACAG ACATGAGCGT 
GAAAAAACGT GTCGCATCGT TGTTATTGAC GACTCCCTGT ATGAAGAGGA GGAAACATTC 
CATGTCCTTC TGAGCGTGCC CATGGGCGGG AGGATTGGAT CAGAATACCC AAGGGCTCGG 
GTTACAATCG TCCCTGACAA AGATGATGAA CCCGTCTTCT ACTTCGGCGA TGTCGAGTAC 
TCCGTGGACG AGAGCGCCGG CTACGTGGAA GTGCGGGTGT GGAGGACGGG CACAGACCTG 
TCCAAGCCTT CCAGTGTCAC CGTGAGGTCT CGGAAAACAG ATCCCCCCTC TGCTGATGCT 
GGAACTGACT ATGTGGGAAT CAGCCGTAAT TTAGATTTTG CACCTGGAGT CAACATGCAG 
ACTGTTCGTG TTACCATCCT GGACGATCTC GGGCAACCAG TGCTGGAAGG AATTGAGAAA 
TTTGAACTGG TGCTTCGTAT GCCGATGAAC GCTGCCCTTG GCGAGCCCAG CAAAGCCACA 
GTGTCCATAA ATGATTCTGT CTCAGATTTG CCTAAGATGC AATTCAAAGA ACGGGTGTAT 
ATTGGCAATG AAAATGATGG GCAGATAGTC GCAATGATCC ACAGGAATGG AGACATTCAC 
TACAGGTCTT CAGTGAGATG CTACACACGC CAGGGGTCTG CACAGGTGAT GGCGGACTTT 
GAGGAACGTC CAAACACTGA TGGCTCCATC ATCACGTTCC TCCCTGGTGA GACTGAAAAG 
CCTTGTGTCC TCGAGCTGAT GGACGACAAA CTCTATGAGG AGGTAGAGGA GCTCCGCCTG 
GTTCTTGGCA CCCCACACAG CAACTCCCCC TTCGGGGCTG CGGTCGGGGA ACAAAATGAA 
ACTCTGATAA GGATCCAAGA TGATGCTGAT AAGGCCATCA TTAAATTTGG AGAAACCAAA 
TTTAGTGTCA GTGAACCCAA AGAACCAGGA CAGTCGGTGG TTATAAAAAT TCCAGTGATT 
CGCCAAGGAG ATACTTCGAA GGTTTCCATC GTGAGAGTCC ACACCAAGGA TGGATCAGCC 
ACCTCTGGAG AAGACTATCA CCCTGTGTCA GAAGAAATTG AGTTTAAGGA GGGAGAAACG 
CAGCATCTTG TTAACATTGA AGTAATCTTC GATGGGATCA GAGAAATGAG AGAGGCCTTC 
ACTGTTCACC TAAAACCCGA TGAGAACATG ATTGCAGAGA CTCAGGTCAC CAAAGCCATT 
GTGTATATAG AAGAAATGAA CAGCATGGCA GACGTCACTT TTCCTTCTGT CCCTCAAATT 
GTGTCATTAC TGATGTATGA TGACACTTCC AGAGCTAAAG ATAGTGCTGG ACCTGTGTCT 
GGCTATCCTG TCGTCTGTAT CACGGCTTGC AACCCTAAAT ACTCAGACTA TGACAAAACA 
GGCTCCATTT GTGCAAGTGA GAACATCAAC GACACCTTGA CCCGGTACCG ATGGCTGACT 
AGCGCTCCCG CTGGCCCTGA TGGCGTGACC AGCCCCATGA GAGAAGTGGA CTTTGACACC 
TTCTTTACGT CATCCAAGAT GATCACCTTA GACTCCGTAT ACTTTCAGCC TGGCTCTCGG 
GTGCAGTGTG CGGCTCGTGC TGTGAATGCC GATGGCAATG AGGGCCTGGA GCTGATGAGC 
CCTGTCGTAA CCATCAGCAG AGAAGAAGGT CTTTGTCAGC CCCGAGTACC AGGGACTGTG 
GGAGCAGAAC CATTCTCCGC TAAACTGCGC TACACGGGCC CCGAGGACCC AGAGTACACA 
AACCTTATCA AGCTCACTGT CACGATGCCA CACGTAGACG GCATGCTCCC TGTGATCTCC 
ACTAAAGAGC TTTCCAACTT CGAGCTCACC CTCAGTCCTG ACGGCTCAAG AGTTGGAAAC 
CACCAGTGCT CCAATCTTCT GGACTATACT GAAGTGAAGA CCCACCATGG TTTCTTGACT 
GATGCTACAA AAAATCCAGA AATAATTGGA GAGACGCATC CTTACCAGTA CAGCTCGTCT 
ATCAGAGGTT CCAGTACCTT ACGCTTCTAT CGAAACCTGA ACCTGGAGGC CTGCTTATGG 
GAGTTTGTTA GCTACTATGA CATGTCAGAA CTTCTGACTG ACTGTGGAGG CACCATCGGG 
ACGGATGGAC AGGTCCTAAA TCTAGTACAG TCCTATGTGA CCCTTCGAGT GCCTCTGTAT 
GTTTCGTACG TGTTCCATTC TCCTGTTGGG GTAGGAGGCT GGCAACATTT TGACTTGAAG 
TCAGAGCTCC GACTGACTTT TGTGTATGAC ACTGCCATCT TGTGGAATGA TGGAATCGGC 
AGCCCACCAG AAGCGGAACT CCAAGGTTCT CTCTATCCCA CCAGCATGCG CATCGGCGAA 
GAGGGGCGCT TGGTCGTGAA CTTCAAGACG GAGGCTCAGT TCCACGGCTT GTTTGTACTC 
TCCCACCCGG CATCCTTCAC GAGCTCAATG ATCATGTCGG CTGACCATCC AGGCCTGACC 
TTTTCCCTGC GCCTCATAAG GAGCGAACCC ACCTACAACC AGCCAGTACA GCAATGGAGC 
TTTGTCTCTG ACTTTGCCGT GCGTGACTAT TCGGGGACCT ACACCGTAAA GCTGCTGCCA 
TGCACCATTC CATCGAATCA AGAGTACCGT CTGCCAGTCA CCTGCAACCC CAGAGAGCCT 
GTCACCTTTG ACCTTGACAT ACGGTTCCAA CAGGTCAGCG ACCCGGTAGC AGCTGAGTTT 
AGCTTGAACA CCCAAATGTA CCTACTCTCC AAGAAGAGTC TCTGGTTGTC TGATGGATCC 
ATGGGATTTG GGCAAGAGAG TGATGTCGCC TTTGCAGAAG GTGATATAAT TTATGGTCGA 
GTCATGGTAG ATCCTGTCCA GAATCTAGGT GATTCCTTTT ACTGCAGCAT TGAGAAGGTG 
TTCCTGTGCA CTGGAGCTGA TGGCTATGTT CCCAAATATA GTCCCACGAA TGCAGAATAT 
GGCTGCTTAG CTGATTCTCC TTCGCTCCTG TATAGATTTA AAATTGTGGA CAAAGCTCAG 
CCAGAGACAC AAGCCACCAG TTTTGGAAAT GTCCTGTTTA ATGCCAGACT AGCAGTGGAT 
GACCCTGAAG CTGTTCTCTT AGTGAATCAG CCTGGATCCG ATGGGTTTAA AGTCGACTCA 
ACAGCACTCT TTCAGGTGGC TCTGGGCCGA GAGTGGTACA TACACACCAT CTACACAGTA 
AGATCGAGAG ACAACGCCAA TCGAGGTATT GGCAAAAGAA GCGTGGAGTA CCAGCACCAC 
TCTTTGGTGA GTTCAGGGAA GCCCCGGGCA GGTACCAAAG GCCGGAAGAA GAGGGAGATG 
AGGAGCCCAC CCCCGCTGGC CTGGGAAATA GGTGCTGAAA ACAACCGGGG GACAAACATC 
CAGCACGTCG CCCTGGACCG CACCAGCAAG AAGCAGATCC CCCGAGGGAG AGTTCCTCCT 
GATGGCGTCC TCCCCCGGGA GCTCAACCCT CCCAGCTCTG AGGTCAGCCT GGCCACGGTG 
GTGGGAGGCA TCGCCGTGGG GTTACTCACC CTCTGCCTTG CCGCCATCAC GGTGACGATG 
TGCCAGAGCA AGAAGGGCGC CCGGAGAAAG GACGCCCTGA AGGGCTCGGG CAGCAGCAAG 
CCCATGATGC CCCCACAGAG CCACCACCAC GACAGCTCAG AGGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM2 ( XM_023250973.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023106741.1 Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

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CloneID	OFb69999
Clone ID Related Accession (Same CDS sequence)	XM_023250973.1
Accession Version	XM_023250973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	FRAS1-related extracellular matrix protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018723.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGCGG CCAGGACCCC CGGGATCGCC TCCCACGCCG GCGGGGTGGC CAGCCCCACC 
AGTTTTCAGC AGATCCCAAC GCTGCCGCCC CGCCTGGTGT TGCTGCTGCT GCTGCTTCTC 
CTGTCACTGG TAAGCTGCGT CCTGGCACAG CCCGCCGCCC CTGGCCCGGC GTTGCTGTCC 
CCGGATCTCG CGGGGGCTGC AGGGGTCCCC GCCGAGGAGG CCATCGTGCT GGCAAACCGT 
GGGCTCCGGG TGCCCTTTGG CCGTGAGGTC TGGCTGGATC CGCTGCAGGA CCTGGTGCTG 
CAGGTGCAGC CGGGGGACCG CTGCGCGGTG ACCGTGCTAG ACAACGACGC GCTGGCCCAG 
CGGCCCGGAC GCTTGAGTCC CAAGCGCTTC CCGTGCGACT TCGGCCCGAG AGAGGTGCGC 
TACTCGCACC TGGGCGCGCG CAGCCCGTCG CGGGATCGCA TTCGGCTGCA GTTGCGCTAC 
GACGCGCCGG GAGGGGCCGC AGTGCTACCC TTGGTGCTGG AGGTGGAGGT GGTCTTCACC 
CAGCTGGAGG TGGTGACTCG GAACCTGCCC CTAGTCGTGG AGGAGCTGCT GGGGACCAGC 
AACGCCCTGG ACGCGCGGAG CCTGGAGTTC GCCTACCAGC CGGAGACCGA GGAGTGTCGC 
GTAGGCATCC TGTCCGGCTT GAGCGCGCTG CCTCGCTATG GGGAGCTCCT CCACTACCCA 
CAGGCCCCGG GTCCAGCCAG CGAGGGGGGC GCCCCGGAGC CTCTCCTGAT GGACTGCAAA 
GCTTTCCAGG AGCTGGGGGT GCGCTACCGT CACACAGCCC CCAGTCGGTC ACCGAACAGG 
GACTGGGTGC CCATGGTGGT TGAGCTGCGC TCTCGGGGTT CGCCTGTGGG CAGACCCCCT 
TTGAAACGTG AGCACTTCCA GGTGCTGGTG AGAATCCGAG GAGGGGCGGA GAACACCGCA 
CCCAGGCCCA GTTTCGTGGC CATGATGATG ATGGAGGTGG ACCAGTTTGT ACTGACGGCT 
CTGACCCCAG ACATGCTGGC AGCCGAGGAT GCCGAGTCGC CCCCTGACCT GTTGATCTTC 
AACCTCACCT CTCCCTTCCA GCCTGGCCAG GGCTACCTAG TGAGTACTGA TGACCGCAGC 
CTGCCCCTCT CCTCCTTTAC TCAGAGGGAT CTGCGGCTCC TGAAGATTGC CTACCAGCCC 
CCCTCTGAGG ACTCTGACCA GGAGCGTCTC TTTGAACTGG AGTTGGAAGC AGTGGACCCG 
GAAGGAGCAG CCTCAGACCC TTTTGCCTTC ATGGTAGTGG TGAAGCCCAT GAACACACTG 
GCTCCCGTGG TCACCCGGAA CACCGGTCTC ATTCTCTATG AGGGCCAGTC TCGGCCCCTG 
ACGGGCCTTG CAGGCAGTGG ACCGCAGAAC TTGGTCGTCA GCGATGAGGA TGACCTAGAG 
GCAGTGCAGC TAGAGGTGGT GGCTGGGCTC CGCCACGGTC ACCTTGTCCT TCTGGGCGCC 
ACCGATGGCA ACTCTGCCCC TAAGACCTTC ACGGTGGCCG AGCTGGCCGC CGGCCAGGTG 
GTCTACCATC ACGATGACAG AGATGGCTCA CTGAGTGACA ACCTTGTACT TAGAATGGTG 
GACGGAGGAC GCAGGCACCA GGTGCAGTTC TTGTTCCCCA TCACCTTGGT GCCTGTGGAT 
GACCAGCCGC CAGTCCTCAA TGCTAACACG GGGCTGACGC TGGCGGAGGG TGAAACGGCG 
CCCATCCTTC CCCTGGCCCT GAGTGCAACG GACATCGACT CAGATGACCC TCTGCTGCTT 
TTTGTGCTGG AGTCGCCTTC TTCGACCATA GGGCATCTGC TTCTCCGCCA AACCCATCCT 
CCCCGCGAGG AAGAGGAGCT GAGCAGGGGC CCTTGGAGGA AACAGGGAGC GTTCTATGAG 
CAAACGGTGA CGGAGTGGAG GCAGCGGGAC ATAACAGAGG GGAGGCTGTT CTACAGGCAC 
TCCGGGCCCC ATAGTCCTGG GCCAGTGATG GACCAGTTCA CATTCCGGGT CCAGGATAAC 
CATGACCCCC CTAATCAGTC TGGACCACAC AGGTTTGTGA TTCGCATCCA TCCTGTGGAT 
CGCCTCCCTC CGGAGCTGGG CAGCGGCTGT CCCCTTCGGA TGGTGGTGCA AGAATCCCAG 
CTCACGCCGC TGAGGAAAAA GTGGCTGCGC TACACTGACC TGGACACAGA TGACCGAGAG 
CTGCGCTACA CCGTGACCCA GCCCCCCACG GACACAGATG AAAACCACTC ACCAGCCCCC 
CTGGGCACCT TGGTCCTGAC CGACAACCCC TCAGTCGTGG TGGCCCATTT TACCCAAGCC 
CAGATCAACC ACCATAAAAT TGCCTACAGA CCTCCGGATC GAGAACTGGG AGTGGCTGCC 
CGAGTGGCCC AGTTTCAATT CCAGGTGGAG GACCAAGCCG GGAATGTGGC TCCAGGCACC 
TTCACCCTTT ACTTGCAGCC CGTGGACAAC CAGCCGCCTG AGATCCTCAA CACGGGTTTC 
ACCGTTCAGG TGAAGGGCCA CCACATCCTG AGTGAGACTG AGTTACGTGT GAATGATGTA 
GACACCGATG TAGCCCACAT CTCTTTCACT CTCACACGGG CACCCGAACA TGGCCACATG 
AGAGTGTCTG GACAGATACT GCATGTGGGG GAGGTCTTCC GCTTGGAAGA TATAAAACAG 
GGTCGGATTT CCTATGTCCA TAATGGGGAC GGGTCCCTGA CTGACAGCTG CTCCTTGGAG 
GTCAGCGACA GACACCACGT GGTGCCCATC ACTCTCAGAG TGAATGTCCG GCCCGCGGGT 
GATGAGGTGC CCATGCTGAG CCTTCCTGCT GGTACTCTGG GGTCATATCT AGACGTTTTA 
GAAAATGGAG CTGCTGAACT CACTGCCAGT GTTATCAAGG GGACTGATGG GGACACTGAT 
GATTTGATGT TGACGTTCCT CTTGGAAGAC CCACCCTTGT ATGGAGAAAT CCTGGTCAAC 
GGAGTTCCGG CGGAGCAGTT TACCCAAAGG GACATCTTAG AGGGCTCTGT CGTCTATGCC 
CACACAAGCG GTGAGATAGG CCTGTTGCCC AAAGGGGATT CTTTTAACCT GAGTCTGTCA 
GATATGTCTC AAGAATGGGT AATAGGTGGC AGTACTATCC AAGGGGTTAC TGTGTGGGTG 
ACCATCCTCC CTGTCGATAG CCAAGCTCCA GAAATTTCTG TAGGTGAACG GTTTATAGTC 
ATGGAAGGTG ACAAAAGTGT TATAACCTCA ACACATATAA GTGCTGAAGA TATTGATTCC 
CTCAATGATG ACATCTTGTG CACTATAGTT ATTCAGCCTA CCTCTGGTTA TGTTGAAAAC 
ATTTCTCCAG CACCAGGCTC TGAGAAATCA AGAGCAGGAA TTGCCATAAG TGCCTTTACC 
CTGAAAGACC TCAGGCAGAG TCATATTAAC TATGTCCAGA GTGTCCACAA AGGGGTAGAA 
CCTGTGGAAG ACCGGTTTAT ATTTCGTTGC TCTGATGGCA TTAACTTTTC AGAGAGACAT 
TTTTTTCCCA TTATAATCAT TCCCACCAAT GATGAACAGC CAGAAATATT TATGAGAGAA 
TTTATGGTGA TGGAAGGCAT GAGTCTGGTG ATCGATACAC CCATCCTCAA TGCTGCTGAT 
GCTGACATTC CCCCGGATGA TCTAACTTTC ACCATTACCC AATTTCCCAC TCGTGGTCAC 
ATCATGAATC AGCTGATAAA TGGCACAGTG TTGGTTGAAA GCTTCACCTT GGACCAGATC 
ATAGAGAGTT CAAGCATTAT TTACGAGCAT GATGATTCTG AGACCCAGGA GGACAGTTTT 
GTGATTAAAC TAACAGACGG TAAGCACTCG GTGGAAAGGA TGGTCCTCAT TATGGTTATC 
CCAGTTGATG ATGAGACCCC CAGAATGACC ATCAATAATG GTCTAGAAAT AGAAATTGGG 
GAAACCAAAA TTATCAACAA CAAAATATTG ATGGCAACTG ATTTAGATTC TGAAGACAAA 
TCTTTGGTTT ATATTATTCG TTATGGGCCG GGACATGGCT TATTACAGAG ACAAAAACCC 
GCAGGTGCCT TTGAAAATAT CACAGTGGGC ATGAATTTTA CCCAGGATGA AGTGGACAGA 
AACTTAATTC AGTACGTCCA TTTTGGACAA GAGGGCATTC GGGACCTAAT TAAATTTGAT 
GTGACTGATG GAATAAATGC GCTTATAGAT CGCTACTTTT ATGTTTCTGT TGGGAGCGTT 
GACATTGTCT TCCCGGATGT GATAAGCAAG GGAGTATCCT TGAAAGAAGG TGGTAAAGTC 
ACCCTCACAA CAGACTTATT AAGTACTAGT GACTTGAACA GTCCTGATGA AAACTTAGTT 
TTTACCATCA CCAGGGCTCC CATGAGAGGT CACTTAGAGT GCACAGATCA ACCTGGAGCA 
TCCATCACAT CGTTCTCTCA GCTCCAACTG GCTGGCAACA AAATCTACTA CATCCACACA 
GCTGACGATG AGGTCAAGAT GGACAGTTTT GAGTTTCAAG TCACTGATGG ACATAACCCT 
GTCTTCCGGA CATTCCGTAT CTCCATTAGC GATGTGGACA ACAAAAAGCC AGTGGTCACC 
ATCCATAACC TGGTTGTCAG TGAGGGTGAA AACAAGCTGA TCACTCCCTT TGAACTCACT 
GTCGAAGATC AAGATACTCC AGACAAACTC CTGAAGTTCA CTGTCACGCA GGTGCCTGTT 
CATGGTCAAC TCCTATTCAA CAATACCAGA CCTGCCATGG TTTTTACCAA GCAAGACCTG 
AACGAAAACT TAATCAGCTA CAGACATGAT GGCACTGAGT CAAATGAAGA CAGCTTCTCC 
TTCACAGTGA CTGATGGTTC CCATACAGAG TTCTATGTTT TCCCCGATAC AATATTTGAA 
ACAAGGAGAC CCCAAGTGAT GAAAATCCAA GTCTTACCTG TTGACGATAG CGTCCCCCAA 
ATTGCAGTGA ACAAAGGGGC CTCTACACTT CGCACTCTGG CTACTGGCCA CTTGGGATTC 
ATGATCACAA GCAAAATATT GAAAGTGGAG GACAGAGACA GCTTACGTTT TTCTCTCAGG 
TTTATTGTGA CGGAGGCCCC TCAACACGGG TATCTCCTCA ACCTGGACAG AGGCAACCAC 
AGTGTCTCTC AGTTTACACA AGCTGACATC GAAGACATGA AAATATGCTA CGTTTTAAAA 
GATGGGGCCA ATGCCACAAG TGATATGTTC CATTTTACAG TTGAAGATGG TGGTGGAAAC 
AAATTAACCA AACAGCATTT TCGTCTGAAT TGGGCATGGA TCTCGTTTGA AAAGGAATAT 
TACCTGATCA ATGAGGATTC CAAATTTCTA GATGTCGTTC TTAAACGCAG AGGTTACTTG 
GGAGAGACTT CATTTATAAG TATCGGCACA AGAGATGGGA CTGCAGAAAA AGACAAAGAC 
TTCAAGGGCA AAGCACAGAA ACAAGTGCAG TTCAACCCAG GCCAGACCAG GGCCACCTGG 
AGAGTGCGGA TCATGAGCGA TGGAGAACAC GAGCAGTCGG AGACCTTCCA GGTGGTTCTC 
TCAGAGCCTG TGCTGGCTGC TCTGGAATTC CCTGCAGTGA CCACGGTGGA GATTGTTGAC 
CCTGGAGATG AGTCGACTGT ATTTATTCCC CAGTCGGAAT ACTCCGTTGA AGAAGATGTG 
GGTGAGCTGT TCATTCCCAT CCGAAGAAGT GGAGACGTGA GCCAGGAATT GATGGTGGTC 
TGTTATACGC AACAAGGTAC GGCAGACGGA ACTGTGCCGA CGTCGGTGCT GTCCTACTCA 
GATTACATAT CCAGACCCGA AGACCACACC AGTGTCATCC ACTTTGACAG ACATGAGCGT 
GAAAAAACGT GTCGCATCGT TGTTATTGAC GACTCCCTGT ATGAAGAGGA GGAAACATTC 
CATGTCCTTC TGAGCGTGCC CATGGGCGGG AGGATTGGAT CAGAATACCC AAGGGCTCGG 
GTTACAATCG TCCCTGACAA AGATGATGAA CCCGTCTTCT ACTTCGGCGA TGTCGAGTAC 
TCCGTGGACG AGAGCGCCGG CTACGTGGAA GTGCGGGTGT GGAGGACGGG CACAGACCTG 
TCCAAGCCTT CCAGTGTCAC CGTGAGGTCT CGGAAAACAG ATCCCCCCTC TGCTGATGCT 
GGAACTGACT ATGTGGGAAT CAGCCGTAAT TTAGATTTTG CACCTGGAGT CAACATGCAG 
ACTGTTCGTG TTACCATCCT GGACGATCTC GGGCAACCAG TGCTGGAAGG AATTGAGAAA 
TTTGAACTGG TGCTTCGTAT GCCGATGAAC GCTGCCCTTG GCGAGCCCAG CAAAGCCACA 
GTGTCCATAA ATGATTCTGT CTCAGATTTG CCTAAGATGC AATTCAAAGA ACGGGTGTAT 
ATTGGCAATG AAAATGATGG GCAGATAGTC GCAATGATCC ACAGGAATGG AGACATTCAC 
TACAGGTCTT CAGTGAGATG CTACACACGC CAGGGGTCTG CACAGGTGAT GGCGGACTTT 
GAGGAACGTC CAAACACTGA TGGCTCCATC ATCACGTTCC TCCCTGGTGA GACTGAAAAG 
CCTTGTGTCC TCGAGCTGAT GGACGACAAA CTCTATGAGG AGGTAGAGGA GCTCCGCCTG 
GTTCTTGGCA CCCCACACAG CAACTCCCCC TTCGGGGCTG CGGTCGGGGA ACAAAATGAA 
ACTCTGATAA GGATCCAAGA TGATGCTGAT AAGGCCATCA TTAAATTTGG AGAAACCAAA 
TTTAGTGTCA GTGAACCCAA AGAACCAGGA CAGTCGGTGG TTATAAAAAT TCCAGTGATT 
CGCCAAGGAG ATACTTCGAA GGTTTCCATC GTGAGAGTCC ACACCAAGGA TGGATCAGCC 
ACCTCTGGAG AAGACTATCA CCCTGTGTCA GAAGAAATTG AGTTTAAGGA GGGAGAAACG 
CAGCATCTTG TTAACATTGA AGTAATCTTC GATGGGATCA GAGAAATGAG AGAGGCCTTC 
ACTGTTCACC TAAAACCCGA TGAGAACATG ATTGCAGAGA CTCAGGTCAC CAAAGCCATT 
GTGTATATAG AAGAAATGAA CAGCATGGCA GACGTCACTT TTCCTTCTGT CCCTCAAATT 
GTGTCATTAC TGATGTATGA TGACACTTCC AGAGCTAAAG ATAGTGCTGG ACCTGTGTCT 
GGCTATCCTG TCGTCTGTAT CACGGCTTGC AACCCTAAAT ACTCAGACTA TGACAAAACA 
GGCTCCATTT GTGCAAGTGA GAACATCAAC GACACCTTGA CCCGGTACCG ATGGCTGACT 
AGCGCTCCCG CTGGCCCTGA TGGCGTGACC AGCCCCATGA GAGAAGTGGA CTTTGACACC 
TTCTTTACGT CATCCAAGAT GATCACCTTA GACTCCGTAT ACTTTCAGCC TGGCTCTCGG 
GTGCAGTGTG CGGCTCGTGC TGTGAATGCC GATGGCAATG AGGGCCTGGA GCTGATGAGC 
CCTGTCGTAA CCATCAGCAG AGAAGAAGGT CTTTGTCAGC CCCGAGTACC AGGGACTGTG 
GGAGCAGAAC CATTCTCCGC TAAACTGCGC TACACGGGCC CCGAGGACCC AGAGTACACA 
AACCTTATCA AGCTCACTGT CACGATGCCA CACGTAGACG GCATGCTCCC TGTGATCTCC 
ACTAAAGAGC TTTCCAACTT CGAGCTCACC CTCAGTCCTG ACGGCTCAAG AGTTGGAAAC 
CACCAGTGCT CCAATCTTCT GGACTATACT GAAGTGAAGA CCCACCATGG TTTCTTGACT 
GATGCTACAA AAAATCCAGA AATAATTGGA GAGACGCATC CTTACCAGTA CAGCTCGTCT 
ATCAGAGGTT CCAGTACCTT ACGCTTCTAT CGAAACCTGA ACCTGGAGGC CTGCTTATGG 
GAGTTTGTTA GCTACTATGA CATGTCAGAA CTTCTGACTG ACTGTGGAGG CACCATCGGG 
ACGGATGGAC AGGTCCTAAA TCTAGTACAG TCCTATGTGA CCCTTCGAGT GCCTCTGTAT 
GTTTCGTACG TGTTCCATTC TCCTGTTGGG GTAGGAGGCT GGCAACATTT TGACTTGAAG 
TCAGAGCTCC GACTGACTTT TGTGTATGAC ACTGCCATCT TGTGGAATGA TGGAATCGGC 
AGCCCACCAG AAGCGGAACT CCAAGGTTCT CTCTATCCCA CCAGCATGCG CATCGGCGAA 
GAGGGGCGCT TGGTCGTGAA CTTCAAGACG GAGGCTCAGT TCCACGGCTT GTTTGTACTC 
TCCCACCCGG CATCCTTCAC GAGCTCAATG ATCATGTCGG CTGACCATCC AGGCCTGACC 
TTTTCCCTGC GCCTCATAAG GAGCGAACCC ACCTACAACC AGCCAGTACA GCAATGGAGC 
TTTGTCTCTG ACTTTGCCGT GCGTGACTAT TCGGGGACCT ACACCGTAAA GCTGCTGCCA 
TGCACCATTC CATCGAATCA AGAGTACCGT CTGCCAGTCA CCTGCAACCC CAGAGAGCCT 
GTCACCTTTG ACCTTGACAT ACGGTTCCAA CAGGTCAGCG ACCCGGTAGC AGCTGAGTTT 
AGCTTGAACA CCCAAATGTA CCTACTCTCC AAGAAGAGTC TCTGGTTGTC TGATGGATCC 
ATGGGATTTG GGCAAGAGAG TGATGTCGCC TTTGCAGAAG GTGATATAAT TTATGGTCGA 
GTCATGGTAG ATCCTGTCCA GAATCTAGGT GATTCCTTTT ACTGCAGCAT TGAGAAGGTG 
TTCCTGTGCA CTGGAGCTGA TGGCTATGTT CCCAAATATA GTCCCACGAA TGCAGAATAT 
GGCTGCTTAG CTGATTCTCC TTCGCTCCTG TATAGATTTA AAATTGTGGA CAAAGCTCAG 
CCAGAGACAC AAGCCACCAG TTTTGGAAAT GTCCTGTTTA ATGCCAGACT AGCAGTGGAT 
GACCCTGAAG CTGTTCTCTT AGTGAATCAG CCTGGATCCG ATGGGTTTAA AGTCGACTCA 
ACAGCACTCT TTCAGGCCCT GGAAAAGCAG AAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023106741.1
CDS	258..9296
Translation	MHAARTPGIASHAGGVASPTSFQQIPTLPPRLVLLLLLLLLSLVSCVLAQPAAPGPALLSPDLAGAAGVPAEEAIVLANRGLRVPFGREVWLDPLQDLVLQVQPGDRCAVTVLDNDALAQRPGRLSPKRFPCDFGPREVRYSHLGARSPSRDRIRLQLRYDAPGGAAVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLSALPRYGELLHYPQAPGPASEGGAPEPLLMDCKAFQELGVRYRHTAPSRSPNRDWVPMVVELRSRGSPVGRPPLKREHFQVLVRIRGGAENTAPRPSFVAMMMMEVDQFVLTALTPDMLAAEDAESPPDLLIFNLTSPFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEAVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQSRPLTGLAGSGPQNLVVSDEDDLEAVQLEVVAGLRHGHLVLLGATDGNSAPKTFTVAELAAGQVVYHHDDRDGSLSDNLVLRMVDGGRRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETAPILPLALSATDIDSDDPLLLFVLESPSSTIGHLLLRQTHPPREEEELSRGPWRKQGAFYEQTVTEWRQRDITEGRLFYRHSGPHSPGPVMDQFTFRVQDNHDPPNQSGPHRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPTDTDENHSPAPLGTLVLTDNPSVVVAHFTQAQINHHKIAYRPPDRELGVAARVAQFQFQVEDQAGNVAPGTFTLYLQPVDNQPPEILNTGFTVQVKGHHILSETELRVNDVDTDVAHISFTLTRAPEHGHMRVSGQILHVGEVFRLEDIKQGRISYVHNGDGSLTDSCSLEVSDRHHVVPITLRVNVRPAGDEVPMLSLPAGTLGSYLDVLENGAAELTASVIKGTDGDTDDLMLTFLLEDPPLYGEILVNGVPAEQFTQRDILEGSVVYAHTSGEIGLLPKGDSFNLSLSDMSQEWVIGGSTIQGVTVWVTILPVDSQAPEISVGERFIVMEGDKSVITSTHISAEDIDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQSHINYVQSVHKGVEPVEDRFIFRCSDGINFSERHFFPIIIIPTNDEQPEIFMREFMVMEGMSLVIDTPILNAADADIPPDDLTFTITQFPTRGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVERMVLIMVIPVDDETPRMTINNGLEIEIGETKIINNKILMATDLDSEDKSLVYIIRYGPGHGLLQRQKPAGAFENITVGMNFTQDEVDRNLIQYVHFGQEGIRDLIKFDVTDGINALIDRYFYVSVGSVDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGASITSFSQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGHNPVFRTFRISISDVDNKKPVVTIHNLVVSEGENKLITPFELTVEDQDTPDKLLKFTVTQVPVHGQLLFNNTRPAMVFTKQDLNENLISYRHDGTESNEDSFSFTVTDGSHTEFYVFPDTIFETRRPQVMKIQVLPVDDSVPQIAVNKGASTLRTLATGHLGFMITSKILKVEDRDSLRFSLRFIVTEAPQHGYLLNLDRGNHSVSQFTQADIEDMKICYVLKDGANATSDMFHFTVEDGGGNKLTKQHFRLNWAWISFEKEYYLINEDSKFLDVVLKRRGYLGETSFISIGTRDGTAEKDKDFKGKAQKQVQFNPGQTRATWRVRIMSDGEHEQSETFQVVLSEPVLAALEFPAVTTVEIVDPGDESTVFIPQSEYSVEEDVGELFIPIRRSGDVSQELMVVCYTQQGTADGTVPTSVLSYSDYISRPEDHTSVIHFDRHEREKTCRIVVIDDSLYEEEETFHVLLSVPMGGRIGSEYPRARVTIVPDKDDEPVFYFGDVEYSVDESAGYVEVRVWRTGTDLSKPSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQTVRVTILDDLGQPVLEGIEKFELVLRMPMNAALGEPSKATVSINDSVSDLPKMQFKERVYIGNENDGQIVAMIHRNGDIHYRSSVRCYTRQGSAQVMADFEERPNTDGSIITFLPGETEKPCVLELMDDKLYEEVEELRLVLGTPHSNSPFGAAVGEQNETLIRIQDDADKAIIKFGETKFSVSEPKEPGQSVVIKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHLVNIEVIFDGIREMREAFTVHLKPDENMIAETQVTKAIVYIEEMNSMADVTFPSVPQIVSLLMYDDTSRAKDSAGPVSGYPVVCITACNPKYSDYDKTGSICASENINDTLTRYRWLTSAPAGPDGVTSPMREVDFDTFFTSSKMITLDSVYFQPGSRVQCAARAVNADGNEGLELMSPVVTISREEGLCQPRVPGTVGAEPFSAKLRYTGPEDPEYTNLIKLTVTMPHVDGMLPVISTKELSNFELTLSPDGSRVGNHQCSNLLDYTEVKTHHGFLTDATKNPEIIGETHPYQYSSSIRGSSTLRFYRNLNLEACLWEFVSYYDMSELLTDCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNDGIGSPPEAELQGSLYPTSMRIGEEGRLVVNFKTEAQFHGLFVLSHPASFTSSMIMSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLLPCTIPSNQEYRLPVTCNPREPVTFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYSPTNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNARLAVDDPEAVLLVNQPGSDGFKVDSTALFQALEKQKN

Target ORF information:

RefSeq Version	XM_023250973.1
Organism	Felis catus(domestic cat)
Definition	Felis catus FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023250973.1

ORF Insert Sequence:

ATGCACGCGG CCAGGACCCC CGGGATCGCC TCCCACGCCG GCGGGGTGGC CAGCCCCACC 
AGTTTTCAGC AGATCCCAAC GCTGCCGCCC CGCCTGGTGT TGCTGCTGCT GCTGCTTCTC 
CTGTCACTGG TAAGCTGCGT CCTGGCACAG CCCGCCGCCC CTGGCCCGGC GTTGCTGTCC 
CCGGATCTCG CGGGGGCTGC AGGGGTCCCC GCCGAGGAGG CCATCGTGCT GGCAAACCGT 
GGGCTCCGGG TGCCCTTTGG CCGTGAGGTC TGGCTGGATC CGCTGCAGGA CCTGGTGCTG 
CAGGTGCAGC CGGGGGACCG CTGCGCGGTG ACCGTGCTAG ACAACGACGC GCTGGCCCAG 
CGGCCCGGAC GCTTGAGTCC CAAGCGCTTC CCGTGCGACT TCGGCCCGAG AGAGGTGCGC 
TACTCGCACC TGGGCGCGCG CAGCCCGTCG CGGGATCGCA TTCGGCTGCA GTTGCGCTAC 
GACGCGCCGG GAGGGGCCGC AGTGCTACCC TTGGTGCTGG AGGTGGAGGT GGTCTTCACC 
CAGCTGGAGG TGGTGACTCG GAACCTGCCC CTAGTCGTGG AGGAGCTGCT GGGGACCAGC 
AACGCCCTGG ACGCGCGGAG CCTGGAGTTC GCCTACCAGC CGGAGACCGA GGAGTGTCGC 
GTAGGCATCC TGTCCGGCTT GAGCGCGCTG CCTCGCTATG GGGAGCTCCT CCACTACCCA 
CAGGCCCCGG GTCCAGCCAG CGAGGGGGGC GCCCCGGAGC CTCTCCTGAT GGACTGCAAA 
GCTTTCCAGG AGCTGGGGGT GCGCTACCGT CACACAGCCC CCAGTCGGTC ACCGAACAGG 
GACTGGGTGC CCATGGTGGT TGAGCTGCGC TCTCGGGGTT CGCCTGTGGG CAGACCCCCT 
TTGAAACGTG AGCACTTCCA GGTGCTGGTG AGAATCCGAG GAGGGGCGGA GAACACCGCA 
CCCAGGCCCA GTTTCGTGGC CATGATGATG ATGGAGGTGG ACCAGTTTGT ACTGACGGCT 
CTGACCCCAG ACATGCTGGC AGCCGAGGAT GCCGAGTCGC CCCCTGACCT GTTGATCTTC 
AACCTCACCT CTCCCTTCCA GCCTGGCCAG GGCTACCTAG TGAGTACTGA TGACCGCAGC 
CTGCCCCTCT CCTCCTTTAC TCAGAGGGAT CTGCGGCTCC TGAAGATTGC CTACCAGCCC 
CCCTCTGAGG ACTCTGACCA GGAGCGTCTC TTTGAACTGG AGTTGGAAGC AGTGGACCCG 
GAAGGAGCAG CCTCAGACCC TTTTGCCTTC ATGGTAGTGG TGAAGCCCAT GAACACACTG 
GCTCCCGTGG TCACCCGGAA CACCGGTCTC ATTCTCTATG AGGGCCAGTC TCGGCCCCTG 
ACGGGCCTTG CAGGCAGTGG ACCGCAGAAC TTGGTCGTCA GCGATGAGGA TGACCTAGAG 
GCAGTGCAGC TAGAGGTGGT GGCTGGGCTC CGCCACGGTC ACCTTGTCCT TCTGGGCGCC 
ACCGATGGCA ACTCTGCCCC TAAGACCTTC ACGGTGGCCG AGCTGGCCGC CGGCCAGGTG 
GTCTACCATC ACGATGACAG AGATGGCTCA CTGAGTGACA ACCTTGTACT TAGAATGGTG 
GACGGAGGAC GCAGGCACCA GGTGCAGTTC TTGTTCCCCA TCACCTTGGT GCCTGTGGAT 
GACCAGCCGC CAGTCCTCAA TGCTAACACG GGGCTGACGC TGGCGGAGGG TGAAACGGCG 
CCCATCCTTC CCCTGGCCCT GAGTGCAACG GACATCGACT CAGATGACCC TCTGCTGCTT 
TTTGTGCTGG AGTCGCCTTC TTCGACCATA GGGCATCTGC TTCTCCGCCA AACCCATCCT 
CCCCGCGAGG AAGAGGAGCT GAGCAGGGGC CCTTGGAGGA AACAGGGAGC GTTCTATGAG 
CAAACGGTGA CGGAGTGGAG GCAGCGGGAC ATAACAGAGG GGAGGCTGTT CTACAGGCAC 
TCCGGGCCCC ATAGTCCTGG GCCAGTGATG GACCAGTTCA CATTCCGGGT CCAGGATAAC 
CATGACCCCC CTAATCAGTC TGGACCACAC AGGTTTGTGA TTCGCATCCA TCCTGTGGAT 
CGCCTCCCTC CGGAGCTGGG CAGCGGCTGT CCCCTTCGGA TGGTGGTGCA AGAATCCCAG 
CTCACGCCGC TGAGGAAAAA GTGGCTGCGC TACACTGACC TGGACACAGA TGACCGAGAG 
CTGCGCTACA CCGTGACCCA GCCCCCCACG GACACAGATG AAAACCACTC ACCAGCCCCC 
CTGGGCACCT TGGTCCTGAC CGACAACCCC TCAGTCGTGG TGGCCCATTT TACCCAAGCC 
CAGATCAACC ACCATAAAAT TGCCTACAGA CCTCCGGATC GAGAACTGGG AGTGGCTGCC 
CGAGTGGCCC AGTTTCAATT CCAGGTGGAG GACCAAGCCG GGAATGTGGC TCCAGGCACC 
TTCACCCTTT ACTTGCAGCC CGTGGACAAC CAGCCGCCTG AGATCCTCAA CACGGGTTTC 
ACCGTTCAGG TGAAGGGCCA CCACATCCTG AGTGAGACTG AGTTACGTGT GAATGATGTA 
GACACCGATG TAGCCCACAT CTCTTTCACT CTCACACGGG CACCCGAACA TGGCCACATG 
AGAGTGTCTG GACAGATACT GCATGTGGGG GAGGTCTTCC GCTTGGAAGA TATAAAACAG 
GGTCGGATTT CCTATGTCCA TAATGGGGAC GGGTCCCTGA CTGACAGCTG CTCCTTGGAG 
GTCAGCGACA GACACCACGT GGTGCCCATC ACTCTCAGAG TGAATGTCCG GCCCGCGGGT 
GATGAGGTGC CCATGCTGAG CCTTCCTGCT GGTACTCTGG GGTCATATCT AGACGTTTTA 
GAAAATGGAG CTGCTGAACT CACTGCCAGT GTTATCAAGG GGACTGATGG GGACACTGAT 
GATTTGATGT TGACGTTCCT CTTGGAAGAC CCACCCTTGT ATGGAGAAAT CCTGGTCAAC 
GGAGTTCCGG CGGAGCAGTT TACCCAAAGG GACATCTTAG AGGGCTCTGT CGTCTATGCC 
CACACAAGCG GTGAGATAGG CCTGTTGCCC AAAGGGGATT CTTTTAACCT GAGTCTGTCA 
GATATGTCTC AAGAATGGGT AATAGGTGGC AGTACTATCC AAGGGGTTAC TGTGTGGGTG 
ACCATCCTCC CTGTCGATAG CCAAGCTCCA GAAATTTCTG TAGGTGAACG GTTTATAGTC 
ATGGAAGGTG ACAAAAGTGT TATAACCTCA ACACATATAA GTGCTGAAGA TATTGATTCC 
CTCAATGATG ACATCTTGTG CACTATAGTT ATTCAGCCTA CCTCTGGTTA TGTTGAAAAC 
ATTTCTCCAG CACCAGGCTC TGAGAAATCA AGAGCAGGAA TTGCCATAAG TGCCTTTACC 
CTGAAAGACC TCAGGCAGAG TCATATTAAC TATGTCCAGA GTGTCCACAA AGGGGTAGAA 
CCTGTGGAAG ACCGGTTTAT ATTTCGTTGC TCTGATGGCA TTAACTTTTC AGAGAGACAT 
TTTTTTCCCA TTATAATCAT TCCCACCAAT GATGAACAGC CAGAAATATT TATGAGAGAA 
TTTATGGTGA TGGAAGGCAT GAGTCTGGTG ATCGATACAC CCATCCTCAA TGCTGCTGAT 
GCTGACATTC CCCCGGATGA TCTAACTTTC ACCATTACCC AATTTCCCAC TCGTGGTCAC 
ATCATGAATC AGCTGATAAA TGGCACAGTG TTGGTTGAAA GCTTCACCTT GGACCAGATC 
ATAGAGAGTT CAAGCATTAT TTACGAGCAT GATGATTCTG AGACCCAGGA GGACAGTTTT 
GTGATTAAAC TAACAGACGG TAAGCACTCG GTGGAAAGGA TGGTCCTCAT TATGGTTATC 
CCAGTTGATG ATGAGACCCC CAGAATGACC ATCAATAATG GTCTAGAAAT AGAAATTGGG 
GAAACCAAAA TTATCAACAA CAAAATATTG ATGGCAACTG ATTTAGATTC TGAAGACAAA 
TCTTTGGTTT ATATTATTCG TTATGGGCCG GGACATGGCT TATTACAGAG ACAAAAACCC 
GCAGGTGCCT TTGAAAATAT CACAGTGGGC ATGAATTTTA CCCAGGATGA AGTGGACAGA 
AACTTAATTC AGTACGTCCA TTTTGGACAA GAGGGCATTC GGGACCTAAT TAAATTTGAT 
GTGACTGATG GAATAAATGC GCTTATAGAT CGCTACTTTT ATGTTTCTGT TGGGAGCGTT 
GACATTGTCT TCCCGGATGT GATAAGCAAG GGAGTATCCT TGAAAGAAGG TGGTAAAGTC 
ACCCTCACAA CAGACTTATT AAGTACTAGT GACTTGAACA GTCCTGATGA AAACTTAGTT 
TTTACCATCA CCAGGGCTCC CATGAGAGGT CACTTAGAGT GCACAGATCA ACCTGGAGCA 
TCCATCACAT CGTTCTCTCA GCTCCAACTG GCTGGCAACA AAATCTACTA CATCCACACA 
GCTGACGATG AGGTCAAGAT GGACAGTTTT GAGTTTCAAG TCACTGATGG ACATAACCCT 
GTCTTCCGGA CATTCCGTAT CTCCATTAGC GATGTGGACA ACAAAAAGCC AGTGGTCACC 
ATCCATAACC TGGTTGTCAG TGAGGGTGAA AACAAGCTGA TCACTCCCTT TGAACTCACT 
GTCGAAGATC AAGATACTCC AGACAAACTC CTGAAGTTCA CTGTCACGCA GGTGCCTGTT 
CATGGTCAAC TCCTATTCAA CAATACCAGA CCTGCCATGG TTTTTACCAA GCAAGACCTG 
AACGAAAACT TAATCAGCTA CAGACATGAT GGCACTGAGT CAAATGAAGA CAGCTTCTCC 
TTCACAGTGA CTGATGGTTC CCATACAGAG TTCTATGTTT TCCCCGATAC AATATTTGAA 
ACAAGGAGAC CCCAAGTGAT GAAAATCCAA GTCTTACCTG TTGACGATAG CGTCCCCCAA 
ATTGCAGTGA ACAAAGGGGC CTCTACACTT CGCACTCTGG CTACTGGCCA CTTGGGATTC 
ATGATCACAA GCAAAATATT GAAAGTGGAG GACAGAGACA GCTTACGTTT TTCTCTCAGG 
TTTATTGTGA CGGAGGCCCC TCAACACGGG TATCTCCTCA ACCTGGACAG AGGCAACCAC 
AGTGTCTCTC AGTTTACACA AGCTGACATC GAAGACATGA AAATATGCTA CGTTTTAAAA 
GATGGGGCCA ATGCCACAAG TGATATGTTC CATTTTACAG TTGAAGATGG TGGTGGAAAC 
AAATTAACCA AACAGCATTT TCGTCTGAAT TGGGCATGGA TCTCGTTTGA AAAGGAATAT 
TACCTGATCA ATGAGGATTC CAAATTTCTA GATGTCGTTC TTAAACGCAG AGGTTACTTG 
GGAGAGACTT CATTTATAAG TATCGGCACA AGAGATGGGA CTGCAGAAAA AGACAAAGAC 
TTCAAGGGCA AAGCACAGAA ACAAGTGCAG TTCAACCCAG GCCAGACCAG GGCCACCTGG 
AGAGTGCGGA TCATGAGCGA TGGAGAACAC GAGCAGTCGG AGACCTTCCA GGTGGTTCTC 
TCAGAGCCTG TGCTGGCTGC TCTGGAATTC CCTGCAGTGA CCACGGTGGA GATTGTTGAC 
CCTGGAGATG AGTCGACTGT ATTTATTCCC CAGTCGGAAT ACTCCGTTGA AGAAGATGTG 
GGTGAGCTGT TCATTCCCAT CCGAAGAAGT GGAGACGTGA GCCAGGAATT GATGGTGGTC 
TGTTATACGC AACAAGGTAC GGCAGACGGA ACTGTGCCGA CGTCGGTGCT GTCCTACTCA 
GATTACATAT CCAGACCCGA AGACCACACC AGTGTCATCC ACTTTGACAG ACATGAGCGT 
GAAAAAACGT GTCGCATCGT TGTTATTGAC GACTCCCTGT ATGAAGAGGA GGAAACATTC 
CATGTCCTTC TGAGCGTGCC CATGGGCGGG AGGATTGGAT CAGAATACCC AAGGGCTCGG 
GTTACAATCG TCCCTGACAA AGATGATGAA CCCGTCTTCT ACTTCGGCGA TGTCGAGTAC 
TCCGTGGACG AGAGCGCCGG CTACGTGGAA GTGCGGGTGT GGAGGACGGG CACAGACCTG 
TCCAAGCCTT CCAGTGTCAC CGTGAGGTCT CGGAAAACAG ATCCCCCCTC TGCTGATGCT 
GGAACTGACT ATGTGGGAAT CAGCCGTAAT TTAGATTTTG CACCTGGAGT CAACATGCAG 
ACTGTTCGTG TTACCATCCT GGACGATCTC GGGCAACCAG TGCTGGAAGG AATTGAGAAA 
TTTGAACTGG TGCTTCGTAT GCCGATGAAC GCTGCCCTTG GCGAGCCCAG CAAAGCCACA 
GTGTCCATAA ATGATTCTGT CTCAGATTTG CCTAAGATGC AATTCAAAGA ACGGGTGTAT 
ATTGGCAATG AAAATGATGG GCAGATAGTC GCAATGATCC ACAGGAATGG AGACATTCAC 
TACAGGTCTT CAGTGAGATG CTACACACGC CAGGGGTCTG CACAGGTGAT GGCGGACTTT 
GAGGAACGTC CAAACACTGA TGGCTCCATC ATCACGTTCC TCCCTGGTGA GACTGAAAAG 
CCTTGTGTCC TCGAGCTGAT GGACGACAAA CTCTATGAGG AGGTAGAGGA GCTCCGCCTG 
GTTCTTGGCA CCCCACACAG CAACTCCCCC TTCGGGGCTG CGGTCGGGGA ACAAAATGAA 
ACTCTGATAA GGATCCAAGA TGATGCTGAT AAGGCCATCA TTAAATTTGG AGAAACCAAA 
TTTAGTGTCA GTGAACCCAA AGAACCAGGA CAGTCGGTGG TTATAAAAAT TCCAGTGATT 
CGCCAAGGAG ATACTTCGAA GGTTTCCATC GTGAGAGTCC ACACCAAGGA TGGATCAGCC 
ACCTCTGGAG AAGACTATCA CCCTGTGTCA GAAGAAATTG AGTTTAAGGA GGGAGAAACG 
CAGCATCTTG TTAACATTGA AGTAATCTTC GATGGGATCA GAGAAATGAG AGAGGCCTTC 
ACTGTTCACC TAAAACCCGA TGAGAACATG ATTGCAGAGA CTCAGGTCAC CAAAGCCATT 
GTGTATATAG AAGAAATGAA CAGCATGGCA GACGTCACTT TTCCTTCTGT CCCTCAAATT 
GTGTCATTAC TGATGTATGA TGACACTTCC AGAGCTAAAG ATAGTGCTGG ACCTGTGTCT 
GGCTATCCTG TCGTCTGTAT CACGGCTTGC AACCCTAAAT ACTCAGACTA TGACAAAACA 
GGCTCCATTT GTGCAAGTGA GAACATCAAC GACACCTTGA CCCGGTACCG ATGGCTGACT 
AGCGCTCCCG CTGGCCCTGA TGGCGTGACC AGCCCCATGA GAGAAGTGGA CTTTGACACC 
TTCTTTACGT CATCCAAGAT GATCACCTTA GACTCCGTAT ACTTTCAGCC TGGCTCTCGG 
GTGCAGTGTG CGGCTCGTGC TGTGAATGCC GATGGCAATG AGGGCCTGGA GCTGATGAGC 
CCTGTCGTAA CCATCAGCAG AGAAGAAGGT CTTTGTCAGC CCCGAGTACC AGGGACTGTG 
GGAGCAGAAC CATTCTCCGC TAAACTGCGC TACACGGGCC CCGAGGACCC AGAGTACACA 
AACCTTATCA AGCTCACTGT CACGATGCCA CACGTAGACG GCATGCTCCC TGTGATCTCC 
ACTAAAGAGC TTTCCAACTT CGAGCTCACC CTCAGTCCTG ACGGCTCAAG AGTTGGAAAC 
CACCAGTGCT CCAATCTTCT GGACTATACT GAAGTGAAGA CCCACCATGG TTTCTTGACT 
GATGCTACAA AAAATCCAGA AATAATTGGA GAGACGCATC CTTACCAGTA CAGCTCGTCT 
ATCAGAGGTT CCAGTACCTT ACGCTTCTAT CGAAACCTGA ACCTGGAGGC CTGCTTATGG 
GAGTTTGTTA GCTACTATGA CATGTCAGAA CTTCTGACTG ACTGTGGAGG CACCATCGGG 
ACGGATGGAC AGGTCCTAAA TCTAGTACAG TCCTATGTGA CCCTTCGAGT GCCTCTGTAT 
GTTTCGTACG TGTTCCATTC TCCTGTTGGG GTAGGAGGCT GGCAACATTT TGACTTGAAG 
TCAGAGCTCC GACTGACTTT TGTGTATGAC ACTGCCATCT TGTGGAATGA TGGAATCGGC 
AGCCCACCAG AAGCGGAACT CCAAGGTTCT CTCTATCCCA CCAGCATGCG CATCGGCGAA 
GAGGGGCGCT TGGTCGTGAA CTTCAAGACG GAGGCTCAGT TCCACGGCTT GTTTGTACTC 
TCCCACCCGG CATCCTTCAC GAGCTCAATG ATCATGTCGG CTGACCATCC AGGCCTGACC 
TTTTCCCTGC GCCTCATAAG GAGCGAACCC ACCTACAACC AGCCAGTACA GCAATGGAGC 
TTTGTCTCTG ACTTTGCCGT GCGTGACTAT TCGGGGACCT ACACCGTAAA GCTGCTGCCA 
TGCACCATTC CATCGAATCA AGAGTACCGT CTGCCAGTCA CCTGCAACCC CAGAGAGCCT 
GTCACCTTTG ACCTTGACAT ACGGTTCCAA CAGGTCAGCG ACCCGGTAGC AGCTGAGTTT 
AGCTTGAACA CCCAAATGTA CCTACTCTCC AAGAAGAGTC TCTGGTTGTC TGATGGATCC 
ATGGGATTTG GGCAAGAGAG TGATGTCGCC TTTGCAGAAG GTGATATAAT TTATGGTCGA 
GTCATGGTAG ATCCTGTCCA GAATCTAGGT GATTCCTTTT ACTGCAGCAT TGAGAAGGTG 
TTCCTGTGCA CTGGAGCTGA TGGCTATGTT CCCAAATATA GTCCCACGAA TGCAGAATAT 
GGCTGCTTAG CTGATTCTCC TTCGCTCCTG TATAGATTTA AAATTGTGGA CAAAGCTCAG 
CCAGAGACAC AAGCCACCAG TTTTGGAAAT GTCCTGTTTA ATGCCAGACT AGCAGTGGAT 
GACCCTGAAG CTGTTCTCTT AGTGAATCAG CCTGGATCCG ATGGGTTTAA AGTCGACTCA 
ACAGCACTCT TTCAGGCCCT GGAAAAGCAG AAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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FREM2 cDNA ORF clone, Felis catus(domestic cat)

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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