ZZEF1 cDNA ORF clone, Felis catus(domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZZEF1
Entrez Gene ID	101086265
Full Name	zinc finger ZZ-type and EF-hand domain containing 1
Gene Type	protein-coding
Organism	Felis catus(domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011288812.1	XP_011287114.1	LOW QUALITY PROTEIN: zinc finger ZZ-type and EF-hand domain-containing protein 1
XM_023243633.1	XP_023099401.1	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X2
XM_023243635.1	XP_023099403.1	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X4
XM_011288812.2	XP_011287114.2	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X3
XM_023243632.1	XP_023099400.1	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following ZZEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZZEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb64463	XM_023243632.1 Latest version!	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFb64464	XM_023243633.1 Latest version!	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFb64465	XM_011288812.2 Latest version!	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFb64466	XM_023243635.1 Latest version!	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZZEF1 ( XM_023243632.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023099400.1 Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb64463
Clone ID Related Accession (Same CDS sequence)	XM_023243632.1
Accession Version	XM_023243632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018736.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAAGA ATGTCACCGA GCAAGAGCAC AAGCAGTCTC TGCAGCTCAC CTTCCGGTCA 
CTGTGCACGT ATTTTAGTGA TAAGGATCCA GGTGGCCTTC TGCTCTTGCC CGAGAAGGGT 
GACGTGGCCA GCATGAACAG CAGTGACGTG CTGGCGGTCA TGAGCGTCCT CCTGTCCGTC 
GCTGCTCGAG AGTGTGAGCT GTTGGTGCTC AGTGGGGCCC AAGGGGAGGC GGGCTCTGTG 
CTCTTCTCCC TGTTCTGGGC GGTCCAAGGC AGCCTGCTGT CCTGGTGCTA CCTGCAGCTG 
AAGAGCACGC ACTCCGGAGC CCGAGAGCTC GCCGCGGACC TCCTTGACCA GTATGTGGCA 
CAGTTTCTGG CGAGCTTGAG AGTGATTTTG GAATCCCTTT TGTCACAGTA TAGTGGAAAG 
ACCCTTGTAG AGAAATTGTG TAATTCCGTG TTTTCGATGG CAGCTCGTCA ACTGGTCATC 
TTTCTCCTGG ACTTCTGCAC TCTGGACATT TCGCACTGTG TGCTCCTGAG AGAGTTCAGC 
ACGCTCCCGG AATTGTTGAA GCAGCTGTGC GGTGACCCTG AAGGAGGGCC AAATACGCTC 
GATGTGGAGC CTTGGCCACA GGAGCGTCCG GTGGTGTTAC ACACGTGGAC GAAGGAGTCT 
GCACACAACT ATGAGAACAA CTGCCACGAG GTGTCCGTCT TTGTTAGCCC GGGCGCAACC 
TGTTTTGAGG TGGAATTTGA TGAGCGGTGT GAGACTGAGA AGCGGTACGA TTATCTGGAA 
TTTACCGACG CTAGAGGTGG AAAGACACGC TATGACACCA AAGTCGGCAC TGACAAGTGG 
CCCAAGAAAG TGACCTTTAA GGCCGGCCCG CGGCTCCAGT TCCTCTTTCA CTCTGACAGC 
AGTAACAATG AGTGGGGCTA CAAGTTCACA GTCACGGCCT ACGGTCTGCC CGACGTGGCC 
GTGTCGTGGG CGTCGGATTT ACAACTGCTT GTTTCCCGGC TGCTGGGACG CCTCGCTGCC 
CAGTGCATGG CGCTCAAGTC TACGCACAAG TTAGGGGGCG ACGTGGCCGT GCCCCCGGCA 
AAAATGGCGG CGGTCCTGAA CTCCCCGCTG TGGAAACCGG TCTTCCGGCA TCAGCTGTGT 
CTGGAGCTGG GATTAGCAGC CAGCTGGCCC ACTAGCCCGC GCCAGGATAG TAAGGAGGTC 
ACGTGCATCC CCGAGGATCC CTGCCGCCGT CTCCTTCTTG AGTTTGTACA GGCAGACCCT 
GCCCAGAGCT GCTGTGGGCC ACTGTCAGAA CTGTGCAAGG GACTCATACA GGCATGTAGA 
AAACAGGCCC CAAAGACAGA TAGAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC 
ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GACTTATATG AGAAGCTGCA GAAATATGTA 
AAAAGTGGGG GCCACACAGC CCTGAGCGAG GAGTTTGCAC AGGTGTACTA TTTGGCAGAT 
GGGATTCGGA TATGGATGCT AGAGATGAAG CAGAAGTCCC TGATGAGCCT GGGGAGCGAG 
GCAGAAGGAA GGCATGGGTC CGAGGCTGCC GAGCTGAACC CCGAGAGCCT GGCGAAAGAG 
TGTATTCAGA AGAGTCTCCT GTTGCTAAGA TTTTTGCCCA TGGGAGTGAG TTCAGAAGAA 
AGCCGTGACA AGTTGGCGGC CACAGATGAC GTGGATCACC TGCAGCCACG CGACAGGCGC 
CCGAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGCTGTCTCC CGTGGAAGCG 
GCCACACCTG CCGTGAGGGA CGGGGGTCCC GGCTCGGAAG CGCAGCCACA CCTGCCCCCC 
GGCAGCGGCC CCCCTGCCTC AGAGGTGCCC CCTGCCGCCC CTGAGGAGCC CTCGTCACCT 
TCCGCTCCCA CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TCCGGCTGCT CTCCTTCCGG 
TCAGTGGAGG AGGCCAGACC GGCGCCCACA GTGAAAGAGA AATACCCCCT GCTGAGGGAT 
GTCCTGGACT TCATCAAGGA CCAGTCCCTC TCCCACGAGA GTGTTGTGAA GGTTCTCTCC 
TGGAGGAAAG CCCAAGCGCA GAGCGTCCTC GAAGTCCTGA GGATAGTTCA GTACTGCGCG 
GAGTCCCTCG GGCAGCCGCA CTGCTTCCAG CCGCCGTGCA TTCTCTTCCT GCTGGAACTT 
CTGACCTGCC AGAAAGAGTT TACCAATTAT TTTGGGCACT TGGAGGGCTG TGGCGCAGAC 
CTGCACAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT CAAAGCGGTT 
AAAGGATTCA GCAGCATAAA CGACAGCGTC CACAAATTGC ACCGTCATGG GCTCTCTAGG 
TCCCTGCTGC CTGCCTTGTC CTGTGTGCAG ACAGCCCTGC TTCATCTTCT GGACATGGGC 
TGGGAACCTG GCGACCTCGC TTTCTTTGTG GACATTCAGC TACCAGATCT CCTCATGAAA 
ATGACACAAG AGAACATAAG TGTCCACGAC AGTGTGATCA GCCAGTGGAG TGAAGACGAT 
GAGCTTGCTG ATGCCAAGCA GAACTCAGAA TGGATGGACG AGTGTCAGGA TGGCATGTTT 
GAGGCCTGGT ATGAAAAAAT AGCCCAGGAA GACCCAGAGA AGCAGAGGAA AATGCACATG 
TTCATTGCCC GCTACTCTGA GCTGCTGCAC GTGGACATCT CCTGCGACGG GTGTGACGAG 
ATCGCGCCCT GGCACCGCTA CCGGTGTCTG CAGTGCAGCG ACATGGATCT CTGCACGACC 
TGCTTCCTAG GTGGGGTGAA GCCCGAGGGC CACGGAGATG ACCACGAGAT GGTCAGCATG 
GAGTTTACCT GTGACCAGTG CCAGGGTTTG ATCATCGGCC GGAGGATGAA TTGCAATGTG 
TGTGATGACT TCGATCTTTG CTATGGGTGC TATGCGGCGA AGAAATACTC CTATGGCCAT 
CTGCCCACCC ACAGTGTCAC GGCTCATCCG ATGGTGACCA TCCGGATCAG CGACCGGCAG 
AGGCTCATCC AGCCTTACAT CCACAACTAC GCCTGGCTGC TCTTCGCCGC GCTGGCTCTC 
TATAGCACCC ACCTGGCCAG CGCAGAGGAT GCGGGCGGTG AGAGGCTGGA TTCCCGGGCC 
CGCAGCAGCG CCGCGGCCCT GCACAGCCAG TGCATGCAGC TTGTGGGGGA CTGTCTGATG 
AAGGCTCACC AGGGAAAAGG TCTGAAAGCC CTAGCTCTTC TCGGGGTACT GCCAGATGGT 
GACGCTAGTC CTCAAAGTCA GGCCCCGGCC GTCGCTGTGC CCGCCCACAC ACCGGAGGAG 
GAGAGAGCGG TCCGGGTCGC TGAGGGCCCG TCACACACTG GCCCTTCTCC GCACGACACT 
GGAAAGAGAG ATGGTCACAA GGTCAGACCT TTCGACTGCA AGCAGAGGAG TAAGGAGGAC 
GAGGGTGCAT CTTTAATGAA GGATCCCGCA CGCCAAACTC AAGTTTCAGA CTCACCTGCA 
GGGCTTCCAG ATGCTGACAC TTCAGTCGTG TCCTCTCAGA AGCCCATCGA GGAAAAGGCA 
GTTACTCCCA GCCCGGAGCA GGTGTTTGCT GAGTGTTCCC AGAAGAGGAT TTTGGGATTA 
CTAGCAGCCA TGTTACCTCC CTTAAAGTTG GACCCCGCGG TCCCCGTGAT AGACCTGGAG 
CGTGCCCTCC CACTCATGTT TCAGGTTGTC ATCTCGAACG CAGGCCACCT GAACGAAACC 
TACCACCTCA CGCTGGGTCT TCTCGGCCAG TTAATCACCC GCCTTTTACC AGCAGAGGTG 
GATGCTGCCG TGGTGAAGGT CCTCTCCGCC AAGCACACCT TGTTTGCAGC GGGGGACAGT 
TCCGTCGTGC CAGATGGCTG GAAGACCACC CACCTGCTCT TCAGCCTGGG AGCCGTGTGT 
CTGGACAGCC GGGTGGGCTT GGACTGGGCC TGCTCCATGG CGGAGATCCT GCGGTCCCTC 
AGCGGTGCCC CCCTGTGGCG TGACGTCATC GCCACCTTCA CGGACCACTG CATCAAGCAG 
CTGCCGTTCC AGCTGAAGCA CACCAACATC TTCACCCTGC TTGTGCTGGT TGGCATCCCC 
CAGGTCCTCT GTGTGGGAAC TCGCTGTGTG TATGTGGATA ATGCCAATGA GCCCCATGAC 
GTGATCATCC TGAAGCACTT CACTGAGAAG AACAGGGCTG TCGTCGTCGA TGTCAAAACC 
CGGAAAAGGA AAACAGTGAA GGACTACCAG CTGGTCCAGA AAGGACAGGA ATGCAACGAC 
TGTCAGGCCC AGCTGAGCCA GTACTCCCAG CACTTTGCCT TTATCGCCAG TCACCTCCTG 
CAAACCAGCA TGGAGAGCCA TTGTCCTGAG GCAGTGGAGG CAACTTGGGT CCTGTCCCTG 
GCCCTCAAAG GACTGTATAA GACACTGAAG GCTCACGGTT TCGAAGAGAC CCATGCGACC 
TTCCTTCAGA CGGATTTGCT GAAGCTGCTG GTGAAAAAGT GCAGCAAAGG AACTGGTTTC 
AGTAAAACGT GGCTCCTCCG GGATCTAGAG ATTTTGTCCA TCATGCTGTA CTCCTCAAAG 
AAGGAGATCC ACACCTCGGT CGAGCACACA GGCCTGGAAC CGGATGAGCG AGGGGATCAA 
GGGGAGGACA CAGAAGGCGC GGTCAGCAGC CCTGGAGAGC AGGAGCAGAG GAAGCTAGAT 
CCTCTGGAAA GCCTGGATGA GCCCACCAGA ATATGTTTCT TGATGGCGCA CGACGCCCTC 
AATGCCCCTC TGCACGTTCT CCGGGCCATA TACGAGCTGC AGATGAAGAG GACCGATTCT 
TTCTTCCTGG AGGTTCAGAA GAGGTTTGAT GGGGATGAGC TCACCACGGA CGAAAGGATC 
CGGACCCTGG CTCAGAGGTG GCAGCCCAGT AGGAGTTTGA GACTGGACGA GCAGAGCGCC 
AAGGCCGTGG ACACAGACAT GATTATCTTA CCGTGTCTGT CGCGGCCCGC ACGCTGTGAC 
CAGGCCACTG CCGAGTCCAA CCCCGTGACC CAGAAGCTGA TCTCCAGCAC GGAGGGCGAA 
CTGCAGCAGA GCTATGCCCA GCAGCGCCGG AGCAAGAGTG CTGCCCTCCT GCACAAGGAG 
TTGGGCTGCA AGAGCAGGAG GGCCGTCCGC GAGTACCTCT TCCGCGTGAA TGAGGCCACG 
GCTGTCCTGT ATGCCCGCCA CGTGCTGGCG TCCTTGCTCG CTGAGTGGCC TGGGCACGTG 
CCCGTGAGCG AGGAGACCCT GGAGCTGAGC GGCCCGGCCC ACATGACCTA CATTTTGGAT 
ATGTTCATGC AGCTGGAAGA AAAGCATCAG TGGGAGAAGA TCCTGCGGAA GGTACTCCAG 
GGCTGCCGAG GGAGCATGCT GGGGACCATG GCCCTGGCTG CCTGCCAGTT CATGGAGGAG 
CCTGGGATGG AAGTGCAAGT GAGGGAGTCC AAACACCCGT ATAACAATAA CACCAACTTT 
GAGGATAAGG TCCACATCCC TGGTGCAATC TACCTCTCAA TCAAATTCGA CGCGCAGTGC 
AGCACAGAGG AGGGCTGTGA CGAGCTGGCC ATGTCCAGCA GCAGTGACTT CCAGCAGGAT 
CGACACACTT TCAGTGGGTC TCAGCAGAAA TGGAAGGATT TTGAGCTTCC AGGAGATACT 
CTGTATTATC GCTTCACCTC TGACATGAGC AACACGGAGT GGGGCTACAA ATTCACTGTG 
ACGGCTGGAC ACCTCGGGCG ATTCCAGACA GGGTTCGAGA TTTTGAAGCA GATGTTGTCA 
GAAGAAAGAG TTGTGCCACA CCTCCCACTG GCCCAGATTT GGGAATGGCT GGTGGGTGTG 
GCTTGTCGCC AGACTGGCCA TCAGCGATTG AAAGCCATTC ACCTGCTTCT GAGGATCGTC 
CGCTGCTGCA CCCACAGCGA CCTCTGTGAC CTGACGCTGC TAAAGCCCCT GTGGCAGCTC 
TTCACGCACA TGGAGTACAG CCTGTTTGAG GACGTGACAC AGCCTGGCAT CCTCCTCCCC 
CTGCACCGTG CCCTCACGGA GCTCTTCCTT GTCACCGAGA ACCGCGCCCA GGAGCTGGGG 
TTGCTGCAGG AGTACCTGCT GGCCTTAACC ACCGACGACC ACCTTCTCCG CTGCGCGGCA 
CAGGCTCTAC AGAACATCGC TGCTATCAGC CTGGCCATCA ACTACCCAAA CAAGGCAACC 
CGCCTCTGGA ATGTCAAGTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023099400.1
CDS	203..9106
Translation	MGNAPSHSSEDEAAAAGGEGWGPHQEWAADSGTIPGPGPAAPALPPAAALLEPARLREAAAALRPAPPCESLVSRHHGALLRWLEERLGRGEESVTLEQFRELLEARGAGCSGEQFEEAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELGHIIRQLQACSLVPGFIDIFSESKEGLGVHASLILRFLHRNRLSGMAVPCPMLDHCNNMCSMRVSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNPADIDKMTNGDTASYWQSDGSARSHWIRLKMKPDVVLRHLSIAVAATDQSYMPQQVTVAVGRSVGELQDVRDVHIPSNVTGYVTLLENANISQLYVQINIKRCLSDGCDTRIHGLRAVGFQRVKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALQHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVELTLLAFALARGSVAKVLSSLCAITDHLDTRYEASSLISSMASVRQNLLLKYGKPLQLILQACDVKGKEEKSGPENLLVEPWTRDGFLTETGKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSEKFHAEEHFKRFEKYDKWKLQEFRQFVKSRAGCSPGDLGEEDPVGWFELEEEWDEADVKLQQCRVAQYLMVKFLCTRQESAERLGVQGLSISGHLRPARTGAEQHVVCTRCVKDTEDSVCGATLLLRTLQFIQQLAHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECICAQTLLLRLLQSCFSVLRSDGPAASQDAKAPRQSPAGAGAAKELHTHLCDVVDRLDGDSVPMETLKQEVKNTLLNGAAIFFPDRQTRRDHLFAMMKNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKGDVASMNSSDVLAVMSVLLSVAARECELLVLSGAQGEAGSVLFSLFWAVQGSLLSWCYLQLKSTHSGARELAADLLDQYVAQFLASLRVILESLLSQYSGKTLVEKLCNSVFSMAARQLVIFLLDFCTLDISHCVLLREFSTLPELLKQLCGDPEGGPNTLDVEPWPQERPVVLHTWTKESAHNYENNCHEVSVFVSPGATCFEVEFDERCETEKRYDYLEFTDARGGKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSNNEWGYKFTVTAYGLPDVAVSWASDLQLLVSRLLGRLAAQCMALKSTHKLGGDVAVPPAKMAAVLNSPLWKPVFRHQLCLELGLAASWPTSPRQDSKEVTCIPEDPCRRLLLEFVQADPAQSCCGPLSELCKGLIQACRKQAPKTDRVAGSTIDQAVNATFAALVCRTPDLYEKLQKYVKSGGHTALSEEFAQVYYLADGIRIWMLEMKQKSLMSLGSEAEGRHGSEAAELNPESLAKECIQKSLLLLRFLPMGVSSEESRDKLAATDDVDHLQPRDRRPRTSSVVEEHFQASLSPVEAATPAVRDGGPGSEAQPHLPPGSGPPASEVPPAAPEEPSSPSAPTRRPPFTRGRLRLLSFRSVEEARPAPTVKEKYPLLRDVLDFIKDQSLSHESVVKVLSWRKAQAQSVLEVLRIVQYCAESLGQPHCFQPPCILFLLELLTCQKEFTNYFGHLEGCGADLHKEIRDTYYQFVLFLVKAVKGFSSINDSVHKLHRHGLSRSLLPALSCVQTALLHLLDMGWEPGDLAFFVDIQLPDLLMKMTQENISVHDSVISQWSEDDELADAKQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYSELLHVDISCDGCDEIAPWHRYRCLQCSDMDLCTTCFLGGVKPEGHGDDHEMVSMEFTCDQCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSVTAHPMVTIRISDRQRLIQPYIHNYAWLLFAALALYSTHLASAEDAGGERLDSRARSSAAALHSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDASPQSQAPAVAVPAHTPEEERAVRVAEGPSHTGPSPHDTGKRDGHKVRPFDCKQRSKEDEGASLMKDPARQTQVSDSPAGLPDADTSVVSSQKPIEEKAVTPSPEQVFAECSQKRILGLLAAMLPPLKLDPAVPVIDLERALPLMFQVVISNAGHLNETYHLTLGLLGQLITRLLPAEVDAAVVKVLSAKHTLFAAGDSSVVPDGWKTTHLLFSLGAVCLDSRVGLDWACSMAEILRSLSGAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGIPQVLCVGTRCVYVDNANEPHDVIILKHFTEKNRAVVVDVKTRKRKTVKDYQLVQKGQECNDCQAQLSQYSQHFAFIASHLLQTSMESHCPEAVEATWVLSLALKGLYKTLKAHGFEETHATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEIHTSVEHTGLEPDERGDQGEDTEGAVSSPGEQEQRKLDPLESLDEPTRICFLMAHDALNAPLHVLRAIYELQMKRTDSFFLEVQKRFDGDELTTDERIRTLAQRWQPSRSLRLDEQSAKAVDTDMIILPCLSRPARCDQATAESNPVTQKLISSTEGELQQSYAQQRRSKSAALLHKELGCKSRRAVREYLFRVNEATAVLYARHVLASLLAEWPGHVPVSEETLELSGPAHMTYILDMFMQLEEKHQWEKILRKVLQGCRGSMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGAIYLSIKFDAQCSTEEGCDELAMSSSSDFQQDRHTFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYKFTVTAGHLGRFQTGFEILKQMLSEERVVPHLPLAQIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCTHSDLCDLTLLKPLWQLFTHMEYSLFEDVTQPGILLPLHRALTELFLVTENRAQELGLLQEYLLALTTDDHLLRCAAQALQNIAAISLAINYPNKATRLWNVKC

Target ORF information:

RefSeq Version	XM_023243632.1
Organism	Felis catus(domestic cat)
Definition	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023243632.1

ORF Insert Sequence:

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAAGA ATGTCACCGA GCAAGAGCAC AAGCAGTCTC TGCAGCTCAC CTTCCGGTCA 
CTGTGCACGT ATTTTAGTGA TAAGGATCCA GGTGGCCTTC TGCTCTTGCC CGAGAAGGGT 
GACGTGGCCA GCATGAACAG CAGTGACGTG CTGGCGGTCA TGAGCGTCCT CCTGTCCGTC 
GCTGCTCGAG AGTGTGAGCT GTTGGTGCTC AGTGGGGCCC AAGGGGAGGC GGGCTCTGTG 
CTCTTCTCCC TGTTCTGGGC GGTCCAAGGC AGCCTGCTGT CCTGGTGCTA CCTGCAGCTG 
AAGAGCACGC ACTCCGGAGC CCGAGAGCTC GCCGCGGACC TCCTTGACCA GTATGTGGCA 
CAGTTTCTGG CGAGCTTGAG AGTGATTTTG GAATCCCTTT TGTCACAGTA TAGTGGAAAG 
ACCCTTGTAG AGAAATTGTG TAATTCCGTG TTTTCGATGG CAGCTCGTCA ACTGGTCATC 
TTTCTCCTGG ACTTCTGCAC TCTGGACATT TCGCACTGTG TGCTCCTGAG AGAGTTCAGC 
ACGCTCCCGG AATTGTTGAA GCAGCTGTGC GGTGACCCTG AAGGAGGGCC AAATACGCTC 
GATGTGGAGC CTTGGCCACA GGAGCGTCCG GTGGTGTTAC ACACGTGGAC GAAGGAGTCT 
GCACACAACT ATGAGAACAA CTGCCACGAG GTGTCCGTCT TTGTTAGCCC GGGCGCAACC 
TGTTTTGAGG TGGAATTTGA TGAGCGGTGT GAGACTGAGA AGCGGTACGA TTATCTGGAA 
TTTACCGACG CTAGAGGTGG AAAGACACGC TATGACACCA AAGTCGGCAC TGACAAGTGG 
CCCAAGAAAG TGACCTTTAA GGCCGGCCCG CGGCTCCAGT TCCTCTTTCA CTCTGACAGC 
AGTAACAATG AGTGGGGCTA CAAGTTCACA GTCACGGCCT ACGGTCTGCC CGACGTGGCC 
GTGTCGTGGG CGTCGGATTT ACAACTGCTT GTTTCCCGGC TGCTGGGACG CCTCGCTGCC 
CAGTGCATGG CGCTCAAGTC TACGCACAAG TTAGGGGGCG ACGTGGCCGT GCCCCCGGCA 
AAAATGGCGG CGGTCCTGAA CTCCCCGCTG TGGAAACCGG TCTTCCGGCA TCAGCTGTGT 
CTGGAGCTGG GATTAGCAGC CAGCTGGCCC ACTAGCCCGC GCCAGGATAG TAAGGAGGTC 
ACGTGCATCC CCGAGGATCC CTGCCGCCGT CTCCTTCTTG AGTTTGTACA GGCAGACCCT 
GCCCAGAGCT GCTGTGGGCC ACTGTCAGAA CTGTGCAAGG GACTCATACA GGCATGTAGA 
AAACAGGCCC CAAAGACAGA TAGAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC 
ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GACTTATATG AGAAGCTGCA GAAATATGTA 
AAAAGTGGGG GCCACACAGC CCTGAGCGAG GAGTTTGCAC AGGTGTACTA TTTGGCAGAT 
GGGATTCGGA TATGGATGCT AGAGATGAAG CAGAAGTCCC TGATGAGCCT GGGGAGCGAG 
GCAGAAGGAA GGCATGGGTC CGAGGCTGCC GAGCTGAACC CCGAGAGCCT GGCGAAAGAG 
TGTATTCAGA AGAGTCTCCT GTTGCTAAGA TTTTTGCCCA TGGGAGTGAG TTCAGAAGAA 
AGCCGTGACA AGTTGGCGGC CACAGATGAC GTGGATCACC TGCAGCCACG CGACAGGCGC 
CCGAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGCTGTCTCC CGTGGAAGCG 
GCCACACCTG CCGTGAGGGA CGGGGGTCCC GGCTCGGAAG CGCAGCCACA CCTGCCCCCC 
GGCAGCGGCC CCCCTGCCTC AGAGGTGCCC CCTGCCGCCC CTGAGGAGCC CTCGTCACCT 
TCCGCTCCCA CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TCCGGCTGCT CTCCTTCCGG 
TCAGTGGAGG AGGCCAGACC GGCGCCCACA GTGAAAGAGA AATACCCCCT GCTGAGGGAT 
GTCCTGGACT TCATCAAGGA CCAGTCCCTC TCCCACGAGA GTGTTGTGAA GGTTCTCTCC 
TGGAGGAAAG CCCAAGCGCA GAGCGTCCTC GAAGTCCTGA GGATAGTTCA GTACTGCGCG 
GAGTCCCTCG GGCAGCCGCA CTGCTTCCAG CCGCCGTGCA TTCTCTTCCT GCTGGAACTT 
CTGACCTGCC AGAAAGAGTT TACCAATTAT TTTGGGCACT TGGAGGGCTG TGGCGCAGAC 
CTGCACAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT CAAAGCGGTT 
AAAGGATTCA GCAGCATAAA CGACAGCGTC CACAAATTGC ACCGTCATGG GCTCTCTAGG 
TCCCTGCTGC CTGCCTTGTC CTGTGTGCAG ACAGCCCTGC TTCATCTTCT GGACATGGGC 
TGGGAACCTG GCGACCTCGC TTTCTTTGTG GACATTCAGC TACCAGATCT CCTCATGAAA 
ATGACACAAG AGAACATAAG TGTCCACGAC AGTGTGATCA GCCAGTGGAG TGAAGACGAT 
GAGCTTGCTG ATGCCAAGCA GAACTCAGAA TGGATGGACG AGTGTCAGGA TGGCATGTTT 
GAGGCCTGGT ATGAAAAAAT AGCCCAGGAA GACCCAGAGA AGCAGAGGAA AATGCACATG 
TTCATTGCCC GCTACTCTGA GCTGCTGCAC GTGGACATCT CCTGCGACGG GTGTGACGAG 
ATCGCGCCCT GGCACCGCTA CCGGTGTCTG CAGTGCAGCG ACATGGATCT CTGCACGACC 
TGCTTCCTAG GTGGGGTGAA GCCCGAGGGC CACGGAGATG ACCACGAGAT GGTCAGCATG 
GAGTTTACCT GTGACCAGTG CCAGGGTTTG ATCATCGGCC GGAGGATGAA TTGCAATGTG 
TGTGATGACT TCGATCTTTG CTATGGGTGC TATGCGGCGA AGAAATACTC CTATGGCCAT 
CTGCCCACCC ACAGTGTCAC GGCTCATCCG ATGGTGACCA TCCGGATCAG CGACCGGCAG 
AGGCTCATCC AGCCTTACAT CCACAACTAC GCCTGGCTGC TCTTCGCCGC GCTGGCTCTC 
TATAGCACCC ACCTGGCCAG CGCAGAGGAT GCGGGCGGTG AGAGGCTGGA TTCCCGGGCC 
CGCAGCAGCG CCGCGGCCCT GCACAGCCAG TGCATGCAGC TTGTGGGGGA CTGTCTGATG 
AAGGCTCACC AGGGAAAAGG TCTGAAAGCC CTAGCTCTTC TCGGGGTACT GCCAGATGGT 
GACGCTAGTC CTCAAAGTCA GGCCCCGGCC GTCGCTGTGC CCGCCCACAC ACCGGAGGAG 
GAGAGAGCGG TCCGGGTCGC TGAGGGCCCG TCACACACTG GCCCTTCTCC GCACGACACT 
GGAAAGAGAG ATGGTCACAA GGTCAGACCT TTCGACTGCA AGCAGAGGAG TAAGGAGGAC 
GAGGGTGCAT CTTTAATGAA GGATCCCGCA CGCCAAACTC AAGTTTCAGA CTCACCTGCA 
GGGCTTCCAG ATGCTGACAC TTCAGTCGTG TCCTCTCAGA AGCCCATCGA GGAAAAGGCA 
GTTACTCCCA GCCCGGAGCA GGTGTTTGCT GAGTGTTCCC AGAAGAGGAT TTTGGGATTA 
CTAGCAGCCA TGTTACCTCC CTTAAAGTTG GACCCCGCGG TCCCCGTGAT AGACCTGGAG 
CGTGCCCTCC CACTCATGTT TCAGGTTGTC ATCTCGAACG CAGGCCACCT GAACGAAACC 
TACCACCTCA CGCTGGGTCT TCTCGGCCAG TTAATCACCC GCCTTTTACC AGCAGAGGTG 
GATGCTGCCG TGGTGAAGGT CCTCTCCGCC AAGCACACCT TGTTTGCAGC GGGGGACAGT 
TCCGTCGTGC CAGATGGCTG GAAGACCACC CACCTGCTCT TCAGCCTGGG AGCCGTGTGT 
CTGGACAGCC GGGTGGGCTT GGACTGGGCC TGCTCCATGG CGGAGATCCT GCGGTCCCTC 
AGCGGTGCCC CCCTGTGGCG TGACGTCATC GCCACCTTCA CGGACCACTG CATCAAGCAG 
CTGCCGTTCC AGCTGAAGCA CACCAACATC TTCACCCTGC TTGTGCTGGT TGGCATCCCC 
CAGGTCCTCT GTGTGGGAAC TCGCTGTGTG TATGTGGATA ATGCCAATGA GCCCCATGAC 
GTGATCATCC TGAAGCACTT CACTGAGAAG AACAGGGCTG TCGTCGTCGA TGTCAAAACC 
CGGAAAAGGA AAACAGTGAA GGACTACCAG CTGGTCCAGA AAGGACAGGA ATGCAACGAC 
TGTCAGGCCC AGCTGAGCCA GTACTCCCAG CACTTTGCCT TTATCGCCAG TCACCTCCTG 
CAAACCAGCA TGGAGAGCCA TTGTCCTGAG GCAGTGGAGG CAACTTGGGT CCTGTCCCTG 
GCCCTCAAAG GACTGTATAA GACACTGAAG GCTCACGGTT TCGAAGAGAC CCATGCGACC 
TTCCTTCAGA CGGATTTGCT GAAGCTGCTG GTGAAAAAGT GCAGCAAAGG AACTGGTTTC 
AGTAAAACGT GGCTCCTCCG GGATCTAGAG ATTTTGTCCA TCATGCTGTA CTCCTCAAAG 
AAGGAGATCC ACACCTCGGT CGAGCACACA GGCCTGGAAC CGGATGAGCG AGGGGATCAA 
GGGGAGGACA CAGAAGGCGC GGTCAGCAGC CCTGGAGAGC AGGAGCAGAG GAAGCTAGAT 
CCTCTGGAAA GCCTGGATGA GCCCACCAGA ATATGTTTCT TGATGGCGCA CGACGCCCTC 
AATGCCCCTC TGCACGTTCT CCGGGCCATA TACGAGCTGC AGATGAAGAG GACCGATTCT 
TTCTTCCTGG AGGTTCAGAA GAGGTTTGAT GGGGATGAGC TCACCACGGA CGAAAGGATC 
CGGACCCTGG CTCAGAGGTG GCAGCCCAGT AGGAGTTTGA GACTGGACGA GCAGAGCGCC 
AAGGCCGTGG ACACAGACAT GATTATCTTA CCGTGTCTGT CGCGGCCCGC ACGCTGTGAC 
CAGGCCACTG CCGAGTCCAA CCCCGTGACC CAGAAGCTGA TCTCCAGCAC GGAGGGCGAA 
CTGCAGCAGA GCTATGCCCA GCAGCGCCGG AGCAAGAGTG CTGCCCTCCT GCACAAGGAG 
TTGGGCTGCA AGAGCAGGAG GGCCGTCCGC GAGTACCTCT TCCGCGTGAA TGAGGCCACG 
GCTGTCCTGT ATGCCCGCCA CGTGCTGGCG TCCTTGCTCG CTGAGTGGCC TGGGCACGTG 
CCCGTGAGCG AGGAGACCCT GGAGCTGAGC GGCCCGGCCC ACATGACCTA CATTTTGGAT 
ATGTTCATGC AGCTGGAAGA AAAGCATCAG TGGGAGAAGA TCCTGCGGAA GGTACTCCAG 
GGCTGCCGAG GGAGCATGCT GGGGACCATG GCCCTGGCTG CCTGCCAGTT CATGGAGGAG 
CCTGGGATGG AAGTGCAAGT GAGGGAGTCC AAACACCCGT ATAACAATAA CACCAACTTT 
GAGGATAAGG TCCACATCCC TGGTGCAATC TACCTCTCAA TCAAATTCGA CGCGCAGTGC 
AGCACAGAGG AGGGCTGTGA CGAGCTGGCC ATGTCCAGCA GCAGTGACTT CCAGCAGGAT 
CGACACACTT TCAGTGGGTC TCAGCAGAAA TGGAAGGATT TTGAGCTTCC AGGAGATACT 
CTGTATTATC GCTTCACCTC TGACATGAGC AACACGGAGT GGGGCTACAA ATTCACTGTG 
ACGGCTGGAC ACCTCGGGCG ATTCCAGACA GGGTTCGAGA TTTTGAAGCA GATGTTGTCA 
GAAGAAAGAG TTGTGCCACA CCTCCCACTG GCCCAGATTT GGGAATGGCT GGTGGGTGTG 
GCTTGTCGCC AGACTGGCCA TCAGCGATTG AAAGCCATTC ACCTGCTTCT GAGGATCGTC 
CGCTGCTGCA CCCACAGCGA CCTCTGTGAC CTGACGCTGC TAAAGCCCCT GTGGCAGCTC 
TTCACGCACA TGGAGTACAG CCTGTTTGAG GACGTGACAC AGCCTGGCAT CCTCCTCCCC 
CTGCACCGTG CCCTCACGGA GCTCTTCCTT GTCACCGAGA ACCGCGCCCA GGAGCTGGGG 
TTGCTGCAGG AGTACCTGCT GGCCTTAACC ACCGACGACC ACCTTCTCCG CTGCGCGGCA 
CAGGCTCTAC AGAACATCGC TGCTATCAGC CTGGCCATCA ACTACCCAAA CAAGGCAACC 
CGCCTCTGGA ATGTCAAGTG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZZEF1 ( XM_023243633.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023099401.1 Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X2, mRNA.

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CloneID	OFb64464
Clone ID Related Accession (Same CDS sequence)	XM_023243633.1
Accession Version	XM_023243633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018736.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAATG TCACCGAGCA AGAGCACAAG CAGTCTCTGC AGCTCACCTT CCGGTCACTG 
TGCACGTATT TTAGTGATAA GGATCCAGGT GGCCTTCTGC TCTTGCCCGA GAAGGGTGAC 
GTGGCCAGCA TGAACAGCAG TGACGTGCTG GCGGTCATGA GCGTCCTCCT GTCCGTCGCT 
GCTCGAGAGT GTGAGCTGTT GGTGCTCAGT GGGGCCCAAG GGGAGGCGGG CTCTGTGCTC 
TTCTCCCTGT TCTGGGCGGT CCAAGGCAGC CTGCTGTCCT GGTGCTACCT GCAGCTGAAG 
AGCACGCACT CCGGAGCCCG AGAGCTCGCC GCGGACCTCC TTGACCAGTA TGTGGCACAG 
TTTCTGGCGA GCTTGAGAGT GATTTTGGAA TCCCTTTTGT CACAGTATAG TGGAAAGACC 
CTTGTAGAGA AATTGTGTAA TTCCGTGTTT TCGATGGCAG CTCGTCAACT GGTCATCTTT 
CTCCTGGACT TCTGCACTCT GGACATTTCG CACTGTGTGC TCCTGAGAGA GTTCAGCACG 
CTCCCGGAAT TGTTGAAGCA GCTGTGCGGT GACCCTGAAG GAGGGCCAAA TACGCTCGAT 
GTGGAGCCTT GGCCACAGGA GCGTCCGGTG GTGTTACACA CGTGGACGAA GGAGTCTGCA 
CACAACTATG AGAACAACTG CCACGAGGTG TCCGTCTTTG TTAGCCCGGG CGCAACCTGT 
TTTGAGGTGG AATTTGATGA GCGGTGTGAG ACTGAGAAGC GGTACGATTA TCTGGAATTT 
ACCGACGCTA GAGGTGGAAA GACACGCTAT GACACCAAAG TCGGCACTGA CAAGTGGCCC 
AAGAAAGTGA CCTTTAAGGC CGGCCCGCGG CTCCAGTTCC TCTTTCACTC TGACAGCAGT 
AACAATGAGT GGGGCTACAA GTTCACAGTC ACGGCCTACG GTCTGCCCGA CGTGGCCGTG 
TCGTGGGCGT CGGATTTACA ACTGCTTGTT TCCCGGCTGC TGGGACGCCT CGCTGCCCAG 
TGCATGGCGC TCAAGTCTAC GCACAAGTTA GGGGGCGACG TGGCCGTGCC CCCGGCAAAA 
ATGGCGGCGG TCCTGAACTC CCCGCTGTGG AAACCGGTCT TCCGGCATCA GCTGTGTCTG 
GAGCTGGGAT TAGCAGCCAG CTGGCCCACT AGCCCGCGCC AGGATAGTAA GGAGGTCACG 
TGCATCCCCG AGGATCCCTG CCGCCGTCTC CTTCTTGAGT TTGTACAGGC AGACCCTGCC 
CAGAGCTGCT GTGGGCCACT GTCAGAACTG TGCAAGGGAC TCATACAGGC ATGTAGAAAA 
CAGGCCCCAA AGACAGATAG AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC 
TTTGCTGCTC TGGTGTGTCG TACTCCAGAC TTATATGAGA AGCTGCAGAA ATATGTAAAA 
AGTGGGGGCC ACACAGCCCT GAGCGAGGAG TTTGCACAGG TGTACTATTT GGCAGATGGG 
ATTCGGATAT GGATGCTAGA GATGAAGCAG AAGTCCCTGA TGAGCCTGGG GAGCGAGGCA 
GAAGGAAGGC ATGGGTCCGA GGCTGCCGAG CTGAACCCCG AGAGCCTGGC GAAAGAGTGT 
ATTCAGAAGA GTCTCCTGTT GCTAAGATTT TTGCCCATGG GAGTGAGTTC AGAAGAAAGC 
CGTGACAAGT TGGCGGCCAC AGATGACGTG GATCACCTGC AGCCACGCGA CAGGCGCCCG 
AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGC TGTCTCCCGT GGAAGCGGCC 
ACACCTGCCG TGAGGGACGG GGGTCCCGGC TCGGAAGCGC AGCCACACCT GCCCCCCGGC 
AGCGGCCCCC CTGCCTCAGA GGTGCCCCCT GCCGCCCCTG AGGAGCCCTC GTCACCTTCC 
GCTCCCACCC GGCGGCCTCC CTTCACCCGA GGGCGACTCC GGCTGCTCTC CTTCCGGTCA 
GTGGAGGAGG CCAGACCGGC GCCCACAGTG AAAGAGAAAT ACCCCCTGCT GAGGGATGTC 
CTGGACTTCA TCAAGGACCA GTCCCTCTCC CACGAGAGTG TTGTGAAGGT TCTCTCCTGG 
AGGAAAGCCC AAGCGCAGAG CGTCCTCGAA GTCCTGAGGA TAGTTCAGTA CTGCGCGGAG 
TCCCTCGGGC AGCCGCACTG CTTCCAGCCG CCGTGCATTC TCTTCCTGCT GGAACTTCTG 
ACCTGCCAGA AAGAGTTTAC CAATTATTTT GGGCACTTGG AGGGCTGTGG CGCAGACCTG 
CACAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTCAA AGCGGTTAAA 
GGATTCAGCA GCATAAACGA CAGCGTCCAC AAATTGCACC GTCATGGGCT CTCTAGGTCC 
CTGCTGCCTG CCTTGTCCTG TGTGCAGACA GCCCTGCTTC ATCTTCTGGA CATGGGCTGG 
GAACCTGGCG ACCTCGCTTT CTTTGTGGAC ATTCAGCTAC CAGATCTCCT CATGAAAATG 
ACACAAGAGA ACATAAGTGT CCACGACAGT GTGATCAGCC AGTGGAGTGA AGACGATGAG 
CTTGCTGATG CCAAGCAGAA CTCAGAATGG ATGGACGAGT GTCAGGATGG CATGTTTGAG 
GCCTGGTATG AAAAAATAGC CCAGGAAGAC CCAGAGAAGC AGAGGAAAAT GCACATGTTC 
ATTGCCCGCT ACTCTGAGCT GCTGCACGTG GACATCTCCT GCGACGGGTG TGACGAGATC 
GCGCCCTGGC ACCGCTACCG GTGTCTGCAG TGCAGCGACA TGGATCTCTG CACGACCTGC 
TTCCTAGGTG GGGTGAAGCC CGAGGGCCAC GGAGATGACC ACGAGATGGT CAGCATGGAG 
TTTACCTGTG ACCAGTGCCA GGGTTTGATC ATCGGCCGGA GGATGAATTG CAATGTGTGT 
GATGACTTCG ATCTTTGCTA TGGGTGCTAT GCGGCGAAGA AATACTCCTA TGGCCATCTG 
CCCACCCACA GTGTCACGGC TCATCCGATG GTGACCATCC GGATCAGCGA CCGGCAGAGG 
CTCATCCAGC CTTACATCCA CAACTACGCC TGGCTGCTCT TCGCCGCGCT GGCTCTCTAT 
AGCACCCACC TGGCCAGCGC AGAGGATGCG GGCGGTGAGA GGCTGGATTC CCGGGCCCGC 
AGCAGCGCCG CGGCCCTGCA CAGCCAGTGC ATGCAGCTTG TGGGGGACTG TCTGATGAAG 
GCTCACCAGG GAAAAGGTCT GAAAGCCCTA GCTCTTCTCG GGGTACTGCC AGATGGTGAC 
GCTAGTCCTC AAAGTCAGGC CCCGGCCGTC GCTGTGCCCG CCCACACACC GGAGGAGGAG 
AGAGCGGTCC GGGTCGCTGA GGGCCCGTCA CACACTGGCC CTTCTCCGCA CGACACTGGA 
AAGAGAGATG GTCACAAGGT CAGACCTTTC GACTGCAAGC AGAGGAGTAA GGAGGACGAG 
GGTGCATCTT TAATGAAGGA TCCCGCACGC CAAACTCAAG TTTCAGACTC ACCTGCAGGG 
CTTCCAGATG CTGACACTTC AGTCGTGTCC TCTCAGAAGC CCATCGAGGA AAAGGCAGTT 
ACTCCCAGCC CGGAGCAGGT GTTTGCTGAG TGTTCCCAGA AGAGGATTTT GGGATTACTA 
GCAGCCATGT TACCTCCCTT AAAGTTGGAC CCCGCGGTCC CCGTGATAGA CCTGGAGCGT 
GCCCTCCCAC TCATGTTTCA GGTTGTCATC TCGAACGCAG GCCACCTGAA CGAAACCTAC 
CACCTCACGC TGGGTCTTCT CGGCCAGTTA ATCACCCGCC TTTTACCAGC AGAGGTGGAT 
GCTGCCGTGG TGAAGGTCCT CTCCGCCAAG CACACCTTGT TTGCAGCGGG GGACAGTTCC 
GTCGTGCCAG ATGGCTGGAA GACCACCCAC CTGCTCTTCA GCCTGGGAGC CGTGTGTCTG 
GACAGCCGGG TGGGCTTGGA CTGGGCCTGC TCCATGGCGG AGATCCTGCG GTCCCTCAGC 
GGTGCCCCCC TGTGGCGTGA CGTCATCGCC ACCTTCACGG ACCACTGCAT CAAGCAGCTG 
CCGTTCCAGC TGAAGCACAC CAACATCTTC ACCCTGCTTG TGCTGGTTGG CATCCCCCAG 
GTCCTCTGTG TGGGAACTCG CTGTGTGTAT GTGGATAATG CCAATGAGCC CCATGACGTG 
ATCATCCTGA AGCACTTCAC TGAGAAGAAC AGGGCTGTCG TCGTCGATGT CAAAACCCGG 
AAAAGGAAAA CAGTGAAGGA CTACCAGCTG GTCCAGAAAG GACAGGAATG CAACGACTGT 
CAGGCCCAGC TGAGCCAGTA CTCCCAGCAC TTTGCCTTTA TCGCCAGTCA CCTCCTGCAA 
ACCAGCATGG AGAGCCATTG TCCTGAGGCA GTGGAGGCAA CTTGGGTCCT GTCCCTGGCC 
CTCAAAGGAC TGTATAAGAC ACTGAAGGCT CACGGTTTCG AAGAGACCCA TGCGACCTTC 
CTTCAGACGG ATTTGCTGAA GCTGCTGGTG AAAAAGTGCA GCAAAGGAAC TGGTTTCAGT 
AAAACGTGGC TCCTCCGGGA TCTAGAGATT TTGTCCATCA TGCTGTACTC CTCAAAGAAG 
GAGATCCACA CCTCGGTCGA GCACACAGGC CTGGAACCGG ATGAGCGAGG GGATCAAGGG 
GAGGACACAG AAGGCGCGGT CAGCAGCCCT GGAGAGCAGG AGCAGAGGAA GCTAGATCCT 
CTGGAAAGCC TGGATGAGCC CACCAGAATA TGTTTCTTGA TGGCGCACGA CGCCCTCAAT 
GCCCCTCTGC ACGTTCTCCG GGCCATATAC GAGCTGCAGA TGAAGAGGAC CGATTCTTTC 
TTCCTGGAGG TTCAGAAGAG GTTTGATGGG GATGAGCTCA CCACGGACGA AAGGATCCGG 
ACCCTGGCTC AGAGGTGGCA GCCCAGTAGG AGTTTGAGAC TGGACGAGCA GAGCGCCAAG 
GCCGTGGACA CAGACATGAT TATCTTACCG TGTCTGTCGC GGCCCGCACG CTGTGACCAG 
GCCACTGCCG AGTCCAACCC CGTGACCCAG AAGCTGATCT CCAGCACGGA GGGCGAACTG 
CAGCAGAGCT ATGCCCAGCA GCGCCGGAGC AAGAGTGCTG CCCTCCTGCA CAAGGAGTTG 
GGCTGCAAGA GCAGGAGGGC CGTCCGCGAG TACCTCTTCC GCGTGAATGA GGCCACGGCT 
GTCCTGTATG CCCGCCACGT GCTGGCGTCC TTGCTCGCTG AGTGGCCTGG GCACGTGCCC 
GTGAGCGAGG AGACCCTGGA GCTGAGCGGC CCGGCCCACA TGACCTACAT TTTGGATATG 
TTCATGCAGC TGGAAGAAAA GCATCAGTGG GAGAAGATCC TGCGGAAGGT ACTCCAGGGC 
TGCCGAGGGA GCATGCTGGG GACCATGGCC CTGGCTGCCT GCCAGTTCAT GGAGGAGCCT 
GGGATGGAAG TGCAAGTGAG GGAGTCCAAA CACCCGTATA ACAATAACAC CAACTTTGAG 
GATAAGGTCC ACATCCCTGG TGCAATCTAC CTCTCAATCA AATTCGACGC GCAGTGCAGC 
ACAGAGGAGG GCTGTGACGA GCTGGCCATG TCCAGCAGCA GTGACTTCCA GCAGGATCGA 
CACACTTTCA GTGGGTCTCA GCAGAAATGG AAGGATTTTG AGCTTCCAGG AGATACTCTG 
TATTATCGCT TCACCTCTGA CATGAGCAAC ACGGAGTGGG GCTACAAATT CACTGTGACG 
GCTGGACACC TCGGGCGATT CCAGACAGGG TTCGAGATTT TGAAGCAGAT GTTGTCAGAA 
GAAAGAGTTG TGCCACACCT CCCACTGGCC CAGATTTGGG AATGGCTGGT GGGTGTGGCT 
TGTCGCCAGA CTGGCCATCA GCGATTGAAA GCCATTCACC TGCTTCTGAG GATCGTCCGC 
TGCTGCACCC ACAGCGACCT CTGTGACCTG ACGCTGCTAA AGCCCCTGTG GCAGCTCTTC 
ACGCACATGG AGTACAGCCT GTTTGAGGAC GTGACACAGC CTGGCATCCT CCTCCCCCTG 
CACCGTGCCC TCACGGAGCT CTTCCTTGTC ACCGAGAACC GCGCCCAGGA GCTGGGGTTG 
CTGCAGGAGT ACCTGCTGGC CTTAACCACC GACGACCACC TTCTCCGCTG CGCGGCACAG 
GCTCTACAGA ACATCGCTGC TATCAGCCTG GCCATCAACT ACCCAAACAA GGCAACCCGC 
CTCTGGAATG TCAAGTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023099401.1
CDS	203..9103
Translation	MGNAPSHSSEDEAAAAGGEGWGPHQEWAADSGTIPGPGPAAPALPPAAALLEPARLREAAAALRPAPPCESLVSRHHGALLRWLEERLGRGEESVTLEQFRELLEARGAGCSGEQFEEAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELGHIIRQLQACSLVPGFIDIFSESKEGLGVHASLILRFLHRNRLSGMAVPCPMLDHCNNMCSMRVSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNPADIDKMTNGDTASYWQSDGSARSHWIRLKMKPDVVLRHLSIAVAATDQSYMPQQVTVAVGRSVGELQDVRDVHIPSNVTGYVTLLENANISQLYVQINIKRCLSDGCDTRIHGLRAVGFQRVKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALQHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVELTLLAFALARGSVAKVLSSLCAITDHLDTRYEASSLISSMASVRQNLLLKYGKPLQLILQACDVKGKEEKSGPENLLVEPWTRDGFLTETGKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSEKFHAEEHFKRFEKYDKWKLQEFRQFVKSRAGCSPGDLGEEDPVGWFELEEEWDEADVKLQQCRVAQYLMVKFLCTRQESAERLGVQGLSISGHLRPARTGAEQHVVCTRCVKDTEDSVCGATLLLRTLQFIQQLAHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECICAQTLLLRLLQSCFSVLRSDGPAASQDAKAPRQSPAGAGAAKELHTHLCDVVDRLDGDSVPMETLKQEVKNTLLNGAAIFFPDRQTRRDHLFAMMNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKGDVASMNSSDVLAVMSVLLSVAARECELLVLSGAQGEAGSVLFSLFWAVQGSLLSWCYLQLKSTHSGARELAADLLDQYVAQFLASLRVILESLLSQYSGKTLVEKLCNSVFSMAARQLVIFLLDFCTLDISHCVLLREFSTLPELLKQLCGDPEGGPNTLDVEPWPQERPVVLHTWTKESAHNYENNCHEVSVFVSPGATCFEVEFDERCETEKRYDYLEFTDARGGKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSNNEWGYKFTVTAYGLPDVAVSWASDLQLLVSRLLGRLAAQCMALKSTHKLGGDVAVPPAKMAAVLNSPLWKPVFRHQLCLELGLAASWPTSPRQDSKEVTCIPEDPCRRLLLEFVQADPAQSCCGPLSELCKGLIQACRKQAPKTDRVAGSTIDQAVNATFAALVCRTPDLYEKLQKYVKSGGHTALSEEFAQVYYLADGIRIWMLEMKQKSLMSLGSEAEGRHGSEAAELNPESLAKECIQKSLLLLRFLPMGVSSEESRDKLAATDDVDHLQPRDRRPRTSSVVEEHFQASLSPVEAATPAVRDGGPGSEAQPHLPPGSGPPASEVPPAAPEEPSSPSAPTRRPPFTRGRLRLLSFRSVEEARPAPTVKEKYPLLRDVLDFIKDQSLSHESVVKVLSWRKAQAQSVLEVLRIVQYCAESLGQPHCFQPPCILFLLELLTCQKEFTNYFGHLEGCGADLHKEIRDTYYQFVLFLVKAVKGFSSINDSVHKLHRHGLSRSLLPALSCVQTALLHLLDMGWEPGDLAFFVDIQLPDLLMKMTQENISVHDSVISQWSEDDELADAKQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYSELLHVDISCDGCDEIAPWHRYRCLQCSDMDLCTTCFLGGVKPEGHGDDHEMVSMEFTCDQCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSVTAHPMVTIRISDRQRLIQPYIHNYAWLLFAALALYSTHLASAEDAGGERLDSRARSSAAALHSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDASPQSQAPAVAVPAHTPEEERAVRVAEGPSHTGPSPHDTGKRDGHKVRPFDCKQRSKEDEGASLMKDPARQTQVSDSPAGLPDADTSVVSSQKPIEEKAVTPSPEQVFAECSQKRILGLLAAMLPPLKLDPAVPVIDLERALPLMFQVVISNAGHLNETYHLTLGLLGQLITRLLPAEVDAAVVKVLSAKHTLFAAGDSSVVPDGWKTTHLLFSLGAVCLDSRVGLDWACSMAEILRSLSGAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGIPQVLCVGTRCVYVDNANEPHDVIILKHFTEKNRAVVVDVKTRKRKTVKDYQLVQKGQECNDCQAQLSQYSQHFAFIASHLLQTSMESHCPEAVEATWVLSLALKGLYKTLKAHGFEETHATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEIHTSVEHTGLEPDERGDQGEDTEGAVSSPGEQEQRKLDPLESLDEPTRICFLMAHDALNAPLHVLRAIYELQMKRTDSFFLEVQKRFDGDELTTDERIRTLAQRWQPSRSLRLDEQSAKAVDTDMIILPCLSRPARCDQATAESNPVTQKLISSTEGELQQSYAQQRRSKSAALLHKELGCKSRRAVREYLFRVNEATAVLYARHVLASLLAEWPGHVPVSEETLELSGPAHMTYILDMFMQLEEKHQWEKILRKVLQGCRGSMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGAIYLSIKFDAQCSTEEGCDELAMSSSSDFQQDRHTFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYKFTVTAGHLGRFQTGFEILKQMLSEERVVPHLPLAQIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCTHSDLCDLTLLKPLWQLFTHMEYSLFEDVTQPGILLPLHRALTELFLVTENRAQELGLLQEYLLALTTDDHLLRCAAQALQNIAAISLAINYPNKATRLWNVKC

Target ORF information:

RefSeq Version	XM_023243633.1
Organism	Felis catus(domestic cat)
Definition	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023243633.1

ORF Insert Sequence:

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAATG TCACCGAGCA AGAGCACAAG CAGTCTCTGC AGCTCACCTT CCGGTCACTG 
TGCACGTATT TTAGTGATAA GGATCCAGGT GGCCTTCTGC TCTTGCCCGA GAAGGGTGAC 
GTGGCCAGCA TGAACAGCAG TGACGTGCTG GCGGTCATGA GCGTCCTCCT GTCCGTCGCT 
GCTCGAGAGT GTGAGCTGTT GGTGCTCAGT GGGGCCCAAG GGGAGGCGGG CTCTGTGCTC 
TTCTCCCTGT TCTGGGCGGT CCAAGGCAGC CTGCTGTCCT GGTGCTACCT GCAGCTGAAG 
AGCACGCACT CCGGAGCCCG AGAGCTCGCC GCGGACCTCC TTGACCAGTA TGTGGCACAG 
TTTCTGGCGA GCTTGAGAGT GATTTTGGAA TCCCTTTTGT CACAGTATAG TGGAAAGACC 
CTTGTAGAGA AATTGTGTAA TTCCGTGTTT TCGATGGCAG CTCGTCAACT GGTCATCTTT 
CTCCTGGACT TCTGCACTCT GGACATTTCG CACTGTGTGC TCCTGAGAGA GTTCAGCACG 
CTCCCGGAAT TGTTGAAGCA GCTGTGCGGT GACCCTGAAG GAGGGCCAAA TACGCTCGAT 
GTGGAGCCTT GGCCACAGGA GCGTCCGGTG GTGTTACACA CGTGGACGAA GGAGTCTGCA 
CACAACTATG AGAACAACTG CCACGAGGTG TCCGTCTTTG TTAGCCCGGG CGCAACCTGT 
TTTGAGGTGG AATTTGATGA GCGGTGTGAG ACTGAGAAGC GGTACGATTA TCTGGAATTT 
ACCGACGCTA GAGGTGGAAA GACACGCTAT GACACCAAAG TCGGCACTGA CAAGTGGCCC 
AAGAAAGTGA CCTTTAAGGC CGGCCCGCGG CTCCAGTTCC TCTTTCACTC TGACAGCAGT 
AACAATGAGT GGGGCTACAA GTTCACAGTC ACGGCCTACG GTCTGCCCGA CGTGGCCGTG 
TCGTGGGCGT CGGATTTACA ACTGCTTGTT TCCCGGCTGC TGGGACGCCT CGCTGCCCAG 
TGCATGGCGC TCAAGTCTAC GCACAAGTTA GGGGGCGACG TGGCCGTGCC CCCGGCAAAA 
ATGGCGGCGG TCCTGAACTC CCCGCTGTGG AAACCGGTCT TCCGGCATCA GCTGTGTCTG 
GAGCTGGGAT TAGCAGCCAG CTGGCCCACT AGCCCGCGCC AGGATAGTAA GGAGGTCACG 
TGCATCCCCG AGGATCCCTG CCGCCGTCTC CTTCTTGAGT TTGTACAGGC AGACCCTGCC 
CAGAGCTGCT GTGGGCCACT GTCAGAACTG TGCAAGGGAC TCATACAGGC ATGTAGAAAA 
CAGGCCCCAA AGACAGATAG AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC 
TTTGCTGCTC TGGTGTGTCG TACTCCAGAC TTATATGAGA AGCTGCAGAA ATATGTAAAA 
AGTGGGGGCC ACACAGCCCT GAGCGAGGAG TTTGCACAGG TGTACTATTT GGCAGATGGG 
ATTCGGATAT GGATGCTAGA GATGAAGCAG AAGTCCCTGA TGAGCCTGGG GAGCGAGGCA 
GAAGGAAGGC ATGGGTCCGA GGCTGCCGAG CTGAACCCCG AGAGCCTGGC GAAAGAGTGT 
ATTCAGAAGA GTCTCCTGTT GCTAAGATTT TTGCCCATGG GAGTGAGTTC AGAAGAAAGC 
CGTGACAAGT TGGCGGCCAC AGATGACGTG GATCACCTGC AGCCACGCGA CAGGCGCCCG 
AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGC TGTCTCCCGT GGAAGCGGCC 
ACACCTGCCG TGAGGGACGG GGGTCCCGGC TCGGAAGCGC AGCCACACCT GCCCCCCGGC 
AGCGGCCCCC CTGCCTCAGA GGTGCCCCCT GCCGCCCCTG AGGAGCCCTC GTCACCTTCC 
GCTCCCACCC GGCGGCCTCC CTTCACCCGA GGGCGACTCC GGCTGCTCTC CTTCCGGTCA 
GTGGAGGAGG CCAGACCGGC GCCCACAGTG AAAGAGAAAT ACCCCCTGCT GAGGGATGTC 
CTGGACTTCA TCAAGGACCA GTCCCTCTCC CACGAGAGTG TTGTGAAGGT TCTCTCCTGG 
AGGAAAGCCC AAGCGCAGAG CGTCCTCGAA GTCCTGAGGA TAGTTCAGTA CTGCGCGGAG 
TCCCTCGGGC AGCCGCACTG CTTCCAGCCG CCGTGCATTC TCTTCCTGCT GGAACTTCTG 
ACCTGCCAGA AAGAGTTTAC CAATTATTTT GGGCACTTGG AGGGCTGTGG CGCAGACCTG 
CACAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTCAA AGCGGTTAAA 
GGATTCAGCA GCATAAACGA CAGCGTCCAC AAATTGCACC GTCATGGGCT CTCTAGGTCC 
CTGCTGCCTG CCTTGTCCTG TGTGCAGACA GCCCTGCTTC ATCTTCTGGA CATGGGCTGG 
GAACCTGGCG ACCTCGCTTT CTTTGTGGAC ATTCAGCTAC CAGATCTCCT CATGAAAATG 
ACACAAGAGA ACATAAGTGT CCACGACAGT GTGATCAGCC AGTGGAGTGA AGACGATGAG 
CTTGCTGATG CCAAGCAGAA CTCAGAATGG ATGGACGAGT GTCAGGATGG CATGTTTGAG 
GCCTGGTATG AAAAAATAGC CCAGGAAGAC CCAGAGAAGC AGAGGAAAAT GCACATGTTC 
ATTGCCCGCT ACTCTGAGCT GCTGCACGTG GACATCTCCT GCGACGGGTG TGACGAGATC 
GCGCCCTGGC ACCGCTACCG GTGTCTGCAG TGCAGCGACA TGGATCTCTG CACGACCTGC 
TTCCTAGGTG GGGTGAAGCC CGAGGGCCAC GGAGATGACC ACGAGATGGT CAGCATGGAG 
TTTACCTGTG ACCAGTGCCA GGGTTTGATC ATCGGCCGGA GGATGAATTG CAATGTGTGT 
GATGACTTCG ATCTTTGCTA TGGGTGCTAT GCGGCGAAGA AATACTCCTA TGGCCATCTG 
CCCACCCACA GTGTCACGGC TCATCCGATG GTGACCATCC GGATCAGCGA CCGGCAGAGG 
CTCATCCAGC CTTACATCCA CAACTACGCC TGGCTGCTCT TCGCCGCGCT GGCTCTCTAT 
AGCACCCACC TGGCCAGCGC AGAGGATGCG GGCGGTGAGA GGCTGGATTC CCGGGCCCGC 
AGCAGCGCCG CGGCCCTGCA CAGCCAGTGC ATGCAGCTTG TGGGGGACTG TCTGATGAAG 
GCTCACCAGG GAAAAGGTCT GAAAGCCCTA GCTCTTCTCG GGGTACTGCC AGATGGTGAC 
GCTAGTCCTC AAAGTCAGGC CCCGGCCGTC GCTGTGCCCG CCCACACACC GGAGGAGGAG 
AGAGCGGTCC GGGTCGCTGA GGGCCCGTCA CACACTGGCC CTTCTCCGCA CGACACTGGA 
AAGAGAGATG GTCACAAGGT CAGACCTTTC GACTGCAAGC AGAGGAGTAA GGAGGACGAG 
GGTGCATCTT TAATGAAGGA TCCCGCACGC CAAACTCAAG TTTCAGACTC ACCTGCAGGG 
CTTCCAGATG CTGACACTTC AGTCGTGTCC TCTCAGAAGC CCATCGAGGA AAAGGCAGTT 
ACTCCCAGCC CGGAGCAGGT GTTTGCTGAG TGTTCCCAGA AGAGGATTTT GGGATTACTA 
GCAGCCATGT TACCTCCCTT AAAGTTGGAC CCCGCGGTCC CCGTGATAGA CCTGGAGCGT 
GCCCTCCCAC TCATGTTTCA GGTTGTCATC TCGAACGCAG GCCACCTGAA CGAAACCTAC 
CACCTCACGC TGGGTCTTCT CGGCCAGTTA ATCACCCGCC TTTTACCAGC AGAGGTGGAT 
GCTGCCGTGG TGAAGGTCCT CTCCGCCAAG CACACCTTGT TTGCAGCGGG GGACAGTTCC 
GTCGTGCCAG ATGGCTGGAA GACCACCCAC CTGCTCTTCA GCCTGGGAGC CGTGTGTCTG 
GACAGCCGGG TGGGCTTGGA CTGGGCCTGC TCCATGGCGG AGATCCTGCG GTCCCTCAGC 
GGTGCCCCCC TGTGGCGTGA CGTCATCGCC ACCTTCACGG ACCACTGCAT CAAGCAGCTG 
CCGTTCCAGC TGAAGCACAC CAACATCTTC ACCCTGCTTG TGCTGGTTGG CATCCCCCAG 
GTCCTCTGTG TGGGAACTCG CTGTGTGTAT GTGGATAATG CCAATGAGCC CCATGACGTG 
ATCATCCTGA AGCACTTCAC TGAGAAGAAC AGGGCTGTCG TCGTCGATGT CAAAACCCGG 
AAAAGGAAAA CAGTGAAGGA CTACCAGCTG GTCCAGAAAG GACAGGAATG CAACGACTGT 
CAGGCCCAGC TGAGCCAGTA CTCCCAGCAC TTTGCCTTTA TCGCCAGTCA CCTCCTGCAA 
ACCAGCATGG AGAGCCATTG TCCTGAGGCA GTGGAGGCAA CTTGGGTCCT GTCCCTGGCC 
CTCAAAGGAC TGTATAAGAC ACTGAAGGCT CACGGTTTCG AAGAGACCCA TGCGACCTTC 
CTTCAGACGG ATTTGCTGAA GCTGCTGGTG AAAAAGTGCA GCAAAGGAAC TGGTTTCAGT 
AAAACGTGGC TCCTCCGGGA TCTAGAGATT TTGTCCATCA TGCTGTACTC CTCAAAGAAG 
GAGATCCACA CCTCGGTCGA GCACACAGGC CTGGAACCGG ATGAGCGAGG GGATCAAGGG 
GAGGACACAG AAGGCGCGGT CAGCAGCCCT GGAGAGCAGG AGCAGAGGAA GCTAGATCCT 
CTGGAAAGCC TGGATGAGCC CACCAGAATA TGTTTCTTGA TGGCGCACGA CGCCCTCAAT 
GCCCCTCTGC ACGTTCTCCG GGCCATATAC GAGCTGCAGA TGAAGAGGAC CGATTCTTTC 
TTCCTGGAGG TTCAGAAGAG GTTTGATGGG GATGAGCTCA CCACGGACGA AAGGATCCGG 
ACCCTGGCTC AGAGGTGGCA GCCCAGTAGG AGTTTGAGAC TGGACGAGCA GAGCGCCAAG 
GCCGTGGACA CAGACATGAT TATCTTACCG TGTCTGTCGC GGCCCGCACG CTGTGACCAG 
GCCACTGCCG AGTCCAACCC CGTGACCCAG AAGCTGATCT CCAGCACGGA GGGCGAACTG 
CAGCAGAGCT ATGCCCAGCA GCGCCGGAGC AAGAGTGCTG CCCTCCTGCA CAAGGAGTTG 
GGCTGCAAGA GCAGGAGGGC CGTCCGCGAG TACCTCTTCC GCGTGAATGA GGCCACGGCT 
GTCCTGTATG CCCGCCACGT GCTGGCGTCC TTGCTCGCTG AGTGGCCTGG GCACGTGCCC 
GTGAGCGAGG AGACCCTGGA GCTGAGCGGC CCGGCCCACA TGACCTACAT TTTGGATATG 
TTCATGCAGC TGGAAGAAAA GCATCAGTGG GAGAAGATCC TGCGGAAGGT ACTCCAGGGC 
TGCCGAGGGA GCATGCTGGG GACCATGGCC CTGGCTGCCT GCCAGTTCAT GGAGGAGCCT 
GGGATGGAAG TGCAAGTGAG GGAGTCCAAA CACCCGTATA ACAATAACAC CAACTTTGAG 
GATAAGGTCC ACATCCCTGG TGCAATCTAC CTCTCAATCA AATTCGACGC GCAGTGCAGC 
ACAGAGGAGG GCTGTGACGA GCTGGCCATG TCCAGCAGCA GTGACTTCCA GCAGGATCGA 
CACACTTTCA GTGGGTCTCA GCAGAAATGG AAGGATTTTG AGCTTCCAGG AGATACTCTG 
TATTATCGCT TCACCTCTGA CATGAGCAAC ACGGAGTGGG GCTACAAATT CACTGTGACG 
GCTGGACACC TCGGGCGATT CCAGACAGGG TTCGAGATTT TGAAGCAGAT GTTGTCAGAA 
GAAAGAGTTG TGCCACACCT CCCACTGGCC CAGATTTGGG AATGGCTGGT GGGTGTGGCT 
TGTCGCCAGA CTGGCCATCA GCGATTGAAA GCCATTCACC TGCTTCTGAG GATCGTCCGC 
TGCTGCACCC ACAGCGACCT CTGTGACCTG ACGCTGCTAA AGCCCCTGTG GCAGCTCTTC 
ACGCACATGG AGTACAGCCT GTTTGAGGAC GTGACACAGC CTGGCATCCT CCTCCCCCTG 
CACCGTGCCC TCACGGAGCT CTTCCTTGTC ACCGAGAACC GCGCCCAGGA GCTGGGGTTG 
CTGCAGGAGT ACCTGCTGGC CTTAACCACC GACGACCACC TTCTCCGCTG CGCGGCACAG 
GCTCTACAGA ACATCGCTGC TATCAGCCTG GCCATCAACT ACCCAAACAA GGCAACCCGC 
CTCTGGAATG TCAAGTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZZEF1 ( XM_011288812.2 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_011287114.2 Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X3, mRNA.

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CloneID	OFb64465
Clone ID Related Accession (Same CDS sequence)	XM_011288812.2
Accession Version	XM_011288812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018736.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_011288812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAAGA ATGTCACCGA GCAAGAGCAC AAGCAGTCTC TGCAGCTCAC CTTCCGGTCA 
CTGTGCACGT ATTTTAGTGA TAAGGATCCA GGTGGCCTTC TGCTCTTGCC CGAGAAGGGT 
GACGTGGCCA GCATGAACAG CAGTGACGTG CTGGCGGTCA TGAGCGTCCT CCTGTCCGTC 
GCTGCTCGAG AGTGTGAGCT GTTGGTGCTC AGTGGGGCCC AAGGGGAGGC GGGCTCTGTG 
CTCTTCTCCC TGTTCTGGGC GGTCCAAGGC AGCCTGCTGT CCTGGTGCTA CCTGCAGCTG 
AAGAGCACGC ACTCCGGAGC CCGAGAGCTC GCCGCGGACC TCCTTGACCA GTATGTGGCA 
CAGTTTCTGG CGAGCTTGAG AGTGATTTTG GAATCCCTTT TGTCACAGTA TAGTGGAAAG 
ACCCTTGTAG AGAAATTGTG TAATTCCGTG TTTTCGATGG CAGCTCGTCA ACTGGTCATC 
TTTCTCCTGG ACTTCTGCAC TCTGGACATT TCGCACTGTG TGCTCCTGAG AGAGTTCAGC 
ACGCTCCCGG AATTGTTGAA GCAGCTGTGC GGTGACCCTG AAGGAGGGCC AAATACGCTC 
GATGTGGAGC CTTGGCCACA GGAGCGTCCG GTGGTGTTAC ACACGTGGAC GAAGGAGTCT 
GCACACAACT ATGAGAACAA CTGCCACGAG GTGTCCGTCT TTGTTAGCCC GGGCGCAACC 
TGTTTTGAGG TGGAATTTGA TGAGCGGTGT GAGACTGAGA AGCGGTACGA TTATCTGGAA 
TTTACCGACG CTAGAGGTGG AAAGACACGC TATGACACCA AAGTCGGCAC TGACAAGTGG 
CCCAAGAAAG TGACCTTTAA GGCCGGCCCG CGGCTCCAGT TCCTCTTTCA CTCTGACAGC 
AGTAACAATG AGTGGGGCTA CAAGTTCACA GTCACGGCCT ACGGTCTGCC CGACGTGGCC 
GTGTCGTGGG CGTCGGATTT ACAACTGCTT GTTTCCCGGC TGCTGGGACG CCTCGCTGCC 
CAGTGCATGG CGCTCAAGTC TACGCACAAG TTAGGGGGCG ACGTGGCCGT GCCCCCGGCA 
AAAATGGCGG CGGTCCTGAA CTCCCCGCTG TGGAAACCGG TCTTCCGGCA TCAGCTGTGT 
CTGGAGCTGG GATTAGCAGC CAGCTGGCCC ACTAGCCCGC GCCAGGATAG TAAGGAGGTC 
ACGTGCATCC CCGAGGATCC CTGCCGCCGT CTCCTTCTTG AGTTTGTACA GGCAGACCCT 
GCCCAGAGCT GCTGTGGGCC ACTGTCAGAA CTGTGCAAGG GACTCATACA GGCATGTAGA 
AAACAGGCCC CAAAGACAGA TAGAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC 
ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GACTTATATG AGAAGCTGCA GAAATATGTA 
AAAAGTGGGG GCCACACAGC CCTGAGCGAG GAGTTTGCAC AGGTGTACTA TTTGGCAGAT 
GGGATTCGGA TATGGATGCT AGAGATGAAG CAGAAGTCCC TGATGAGCCT GGGGAGCGAG 
GCAGAAGGAA GGCATGGGTC CGAGGCTGCC GAGCTGAACC CCGAGAGCCT GGCGAAAGAG 
TGTATTCAGA AGAGTCTCCT GTTGCTAAGA TTTTTGCCCA TGGGAGTGAG TTCAGAAGAA 
AGCCGTGACA AGTTGGCGGC CACAGATGAC GTGGATCACC TGCAGCCACG CGACAGGCGC 
CCGAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGCTGTCTCC CGTGGAAGCG 
GCCACACCTG CCGTGAGGGA CGGGGGTCCC GGCTCGGAAG CGCAGCCACA CCTGCCCCCC 
GGCAGCGGCC CCCCTGCCTC AGAGGTGCCC CCTGCCGCCC CTGAGGAGCC CTCGTCACCT 
TCCGCTCCCA CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TCCGGCTGCT CTCCTTCCGG 
TCAGTGGAGG AGGCCAGACC GGCGCCCACA GTGAAAGAGA AATACCCCCT GCTGAGGGAT 
GTCCTGGACT TCATCAAGGA CCAGTCCCTC TCCCACGAGA GTGTTGTGAA GGTTCTCTCC 
TGGAGGAAAG CCCAAGCGCA GAGCGTCCTC GAAGTCCTGA GGATAGTTCA GTACTGCGCG 
GAGTCCCTCG GGCAGCCGCA CTGCTTCCAG CCGCCGTGCA TTCTCTTCCT GCTGGAACTT 
CTGACCTGCC AGAAAGAGTT TACCAATTAT TTTGGGCACT TGGAGGGCTG TGGCGCAGAC 
CTGCACAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT CAAAGCGGTT 
AAAGGATTCA GCAGCATAAA CGACAGGTCC CTGCTGCCTG CCTTGTCCTG TGTGCAGACA 
GCCCTGCTTC ATCTTCTGGA CATGGGCTGG GAACCTGGCG ACCTCGCTTT CTTTGTGGAC 
ATTCAGCTAC CAGATCTCCT CATGAAAATG ACACAAGAGA ACATAAGTGT CCACGACAGT 
GTGATCAGCC AGTGGAGTGA AGACGATGAG CTTGCTGATG CCAAGCAGAA CTCAGAATGG 
ATGGACGAGT GTCAGGATGG CATGTTTGAG GCCTGGTATG AAAAAATAGC CCAGGAAGAC 
CCAGAGAAGC AGAGGAAAAT GCACATGTTC ATTGCCCGCT ACTCTGAGCT GCTGCACGTG 
GACATCTCCT GCGACGGGTG TGACGAGATC GCGCCCTGGC ACCGCTACCG GTGTCTGCAG 
TGCAGCGACA TGGATCTCTG CACGACCTGC TTCCTAGGTG GGGTGAAGCC CGAGGGCCAC 
GGAGATGACC ACGAGATGGT CAGCATGGAG TTTACCTGTG ACCAGTGCCA GGGTTTGATC 
ATCGGCCGGA GGATGAATTG CAATGTGTGT GATGACTTCG ATCTTTGCTA TGGGTGCTAT 
GCGGCGAAGA AATACTCCTA TGGCCATCTG CCCACCCACA GTGTCACGGC TCATCCGATG 
GTGACCATCC GGATCAGCGA CCGGCAGAGG CTCATCCAGC CTTACATCCA CAACTACGCC 
TGGCTGCTCT TCGCCGCGCT GGCTCTCTAT AGCACCCACC TGGCCAGCGC AGAGGATGCG 
GGCGGTGAGA GGCTGGATTC CCGGGCCCGC AGCAGCGCCG CGGCCCTGCA CAGCCAGTGC 
ATGCAGCTTG TGGGGGACTG TCTGATGAAG GCTCACCAGG GAAAAGGTCT GAAAGCCCTA 
GCTCTTCTCG GGGTACTGCC AGATGGTGAC GCTAGTCCTC AAAGTCAGGC CCCGGCCGTC 
GCTGTGCCCG CCCACACACC GGAGGAGGAG AGAGCGGTCC GGGTCGCTGA GGGCCCGTCA 
CACACTGGCC CTTCTCCGCA CGACACTGGA AAGAGAGATG GTCACAAGGT CAGACCTTTC 
GACTGCAAGC AGAGGAGTAA GGAGGACGAG GGTGCATCTT TAATGAAGGA TCCCGCACGC 
CAAACTCAAG TTTCAGACTC ACCTGCAGGG CTTCCAGATG CTGACACTTC AGTCGTGTCC 
TCTCAGAAGC CCATCGAGGA AAAGGCAGTT ACTCCCAGCC CGGAGCAGGT GTTTGCTGAG 
TGTTCCCAGA AGAGGATTTT GGGATTACTA GCAGCCATGT TACCTCCCTT AAAGTTGGAC 
CCCGCGGTCC CCGTGATAGA CCTGGAGCGT GCCCTCCCAC TCATGTTTCA GGTTGTCATC 
TCGAACGCAG GCCACCTGAA CGAAACCTAC CACCTCACGC TGGGTCTTCT CGGCCAGTTA 
ATCACCCGCC TTTTACCAGC AGAGGTGGAT GCTGCCGTGG TGAAGGTCCT CTCCGCCAAG 
CACACCTTGT TTGCAGCGGG GGACAGTTCC GTCGTGCCAG ATGGCTGGAA GACCACCCAC 
CTGCTCTTCA GCCTGGGAGC CGTGTGTCTG GACAGCCGGG TGGGCTTGGA CTGGGCCTGC 
TCCATGGCGG AGATCCTGCG GTCCCTCAGC GGTGCCCCCC TGTGGCGTGA CGTCATCGCC 
ACCTTCACGG ACCACTGCAT CAAGCAGCTG CCGTTCCAGC TGAAGCACAC CAACATCTTC 
ACCCTGCTTG TGCTGGTTGG CATCCCCCAG GTCCTCTGTG TGGGAACTCG CTGTGTGTAT 
GTGGATAATG CCAATGAGCC CCATGACGTG ATCATCCTGA AGCACTTCAC TGAGAAGAAC 
AGGGCTGTCG TCGTCGATGT CAAAACCCGG AAAAGGAAAA CAGTGAAGGA CTACCAGCTG 
GTCCAGAAAG GACAGGAATG CAACGACTGT CAGGCCCAGC TGAGCCAGTA CTCCCAGCAC 
TTTGCCTTTA TCGCCAGTCA CCTCCTGCAA ACCAGCATGG AGAGCCATTG TCCTGAGGCA 
GTGGAGGCAA CTTGGGTCCT GTCCCTGGCC CTCAAAGGAC TGTATAAGAC ACTGAAGGCT 
CACGGTTTCG AAGAGACCCA TGCGACCTTC CTTCAGACGG ATTTGCTGAA GCTGCTGGTG 
AAAAAGTGCA GCAAAGGAAC TGGTTTCAGT AAAACGTGGC TCCTCCGGGA TCTAGAGATT 
TTGTCCATCA TGCTGTACTC CTCAAAGAAG GAGATCCACA CCTCGGTCGA GCACACAGGC 
CTGGAACCGG ATGAGCGAGG GGATCAAGGG GAGGACACAG AAGGCGCGGT CAGCAGCCCT 
GGAGAGCAGG AGCAGAGGAA GCTAGATCCT CTGGAAAGCC TGGATGAGCC CACCAGAATA 
TGTTTCTTGA TGGCGCACGA CGCCCTCAAT GCCCCTCTGC ACGTTCTCCG GGCCATATAC 
GAGCTGCAGA TGAAGAGGAC CGATTCTTTC TTCCTGGAGG TTCAGAAGAG GTTTGATGGG 
GATGAGCTCA CCACGGACGA AAGGATCCGG ACCCTGGCTC AGAGGTGGCA GCCCAGTAGG 
AGTTTGAGAC TGGACGAGCA GAGCGCCAAG GCCGTGGACA CAGACATGAT TATCTTACCG 
TGTCTGTCGC GGCCCGCACG CTGTGACCAG GCCACTGCCG AGTCCAACCC CGTGACCCAG 
AAGCTGATCT CCAGCACGGA GGGCGAACTG CAGCAGAGCT ATGCCCAGCA GCGCCGGAGC 
AAGAGTGCTG CCCTCCTGCA CAAGGAGTTG GGCTGCAAGA GCAGGAGGGC CGTCCGCGAG 
TACCTCTTCC GCGTGAATGA GGCCACGGCT GTCCTGTATG CCCGCCACGT GCTGGCGTCC 
TTGCTCGCTG AGTGGCCTGG GCACGTGCCC GTGAGCGAGG AGACCCTGGA GCTGAGCGGC 
CCGGCCCACA TGACCTACAT TTTGGATATG TTCATGCAGC TGGAAGAAAA GCATCAGTGG 
GAGAAGATCC TGCGGAAGGT ACTCCAGGGC TGCCGAGGGA GCATGCTGGG GACCATGGCC 
CTGGCTGCCT GCCAGTTCAT GGAGGAGCCT GGGATGGAAG TGCAAGTGAG GGAGTCCAAA 
CACCCGTATA ACAATAACAC CAACTTTGAG GATAAGGTCC ACATCCCTGG TGCAATCTAC 
CTCTCAATCA AATTCGACGC GCAGTGCAGC ACAGAGGAGG GCTGTGACGA GCTGGCCATG 
TCCAGCAGCA GTGACTTCCA GCAGGATCGA CACACTTTCA GTGGGTCTCA GCAGAAATGG 
AAGGATTTTG AGCTTCCAGG AGATACTCTG TATTATCGCT TCACCTCTGA CATGAGCAAC 
ACGGAGTGGG GCTACAAATT CACTGTGACG GCTGGACACC TCGGGCGATT CCAGACAGGG 
TTCGAGATTT TGAAGCAGAT GTTGTCAGAA GAAAGAGTTG TGCCACACCT CCCACTGGCC 
CAGATTTGGG AATGGCTGGT GGGTGTGGCT TGTCGCCAGA CTGGCCATCA GCGATTGAAA 
GCCATTCACC TGCTTCTGAG GATCGTCCGC TGCTGCACCC ACAGCGACCT CTGTGACCTG 
ACGCTGCTAA AGCCCCTGTG GCAGCTCTTC ACGCACATGG AGTACAGCCT GTTTGAGGAC 
GTGACACAGC CTGGCATCCT CCTCCCCCTG CACCGTGCCC TCACGGAGCT CTTCCTTGTC 
ACCGAGAACC GCGCCCAGGA GCTGGGGTTG CTGCAGGAGT ACCTGCTGGC CTTAACCACC 
GACGACCACC TTCTCCGCTG CGCGGCACAG GCTCTACAGA ACATCGCTGC TATCAGCCTG 
GCCATCAACT ACCCAAACAA GGCAACCCGC CTCTGGAATG TCAAGTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011287114.2
CDS	203..9073
Translation	MGNAPSHSSEDEAAAAGGEGWGPHQEWAADSGTIPGPGPAAPALPPAAALLEPARLREAAAALRPAPPCESLVSRHHGALLRWLEERLGRGEESVTLEQFRELLEARGAGCSGEQFEEAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELGHIIRQLQACSLVPGFIDIFSESKEGLGVHASLILRFLHRNRLSGMAVPCPMLDHCNNMCSMRVSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNPADIDKMTNGDTASYWQSDGSARSHWIRLKMKPDVVLRHLSIAVAATDQSYMPQQVTVAVGRSVGELQDVRDVHIPSNVTGYVTLLENANISQLYVQINIKRCLSDGCDTRIHGLRAVGFQRVKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALQHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVELTLLAFALARGSVAKVLSSLCAITDHLDTRYEASSLISSMASVRQNLLLKYGKPLQLILQACDVKGKEEKSGPENLLVEPWTRDGFLTETGKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSEKFHAEEHFKRFEKYDKWKLQEFRQFVKSRAGCSPGDLGEEDPVGWFELEEEWDEADVKLQQCRVAQYLMVKFLCTRQESAERLGVQGLSISGHLRPARTGAEQHVVCTRCVKDTEDSVCGATLLLRTLQFIQQLAHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECICAQTLLLRLLQSCFSVLRSDGPAASQDAKAPRQSPAGAGAAKELHTHLCDVVDRLDGDSVPMETLKQEVKNTLLNGAAIFFPDRQTRRDHLFAMMKNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKGDVASMNSSDVLAVMSVLLSVAARECELLVLSGAQGEAGSVLFSLFWAVQGSLLSWCYLQLKSTHSGARELAADLLDQYVAQFLASLRVILESLLSQYSGKTLVEKLCNSVFSMAARQLVIFLLDFCTLDISHCVLLREFSTLPELLKQLCGDPEGGPNTLDVEPWPQERPVVLHTWTKESAHNYENNCHEVSVFVSPGATCFEVEFDERCETEKRYDYLEFTDARGGKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSNNEWGYKFTVTAYGLPDVAVSWASDLQLLVSRLLGRLAAQCMALKSTHKLGGDVAVPPAKMAAVLNSPLWKPVFRHQLCLELGLAASWPTSPRQDSKEVTCIPEDPCRRLLLEFVQADPAQSCCGPLSELCKGLIQACRKQAPKTDRVAGSTIDQAVNATFAALVCRTPDLYEKLQKYVKSGGHTALSEEFAQVYYLADGIRIWMLEMKQKSLMSLGSEAEGRHGSEAAELNPESLAKECIQKSLLLLRFLPMGVSSEESRDKLAATDDVDHLQPRDRRPRTSSVVEEHFQASLSPVEAATPAVRDGGPGSEAQPHLPPGSGPPASEVPPAAPEEPSSPSAPTRRPPFTRGRLRLLSFRSVEEARPAPTVKEKYPLLRDVLDFIKDQSLSHESVVKVLSWRKAQAQSVLEVLRIVQYCAESLGQPHCFQPPCILFLLELLTCQKEFTNYFGHLEGCGADLHKEIRDTYYQFVLFLVKAVKGFSSINDRSLLPALSCVQTALLHLLDMGWEPGDLAFFVDIQLPDLLMKMTQENISVHDSVISQWSEDDELADAKQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYSELLHVDISCDGCDEIAPWHRYRCLQCSDMDLCTTCFLGGVKPEGHGDDHEMVSMEFTCDQCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSVTAHPMVTIRISDRQRLIQPYIHNYAWLLFAALALYSTHLASAEDAGGERLDSRARSSAAALHSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDASPQSQAPAVAVPAHTPEEERAVRVAEGPSHTGPSPHDTGKRDGHKVRPFDCKQRSKEDEGASLMKDPARQTQVSDSPAGLPDADTSVVSSQKPIEEKAVTPSPEQVFAECSQKRILGLLAAMLPPLKLDPAVPVIDLERALPLMFQVVISNAGHLNETYHLTLGLLGQLITRLLPAEVDAAVVKVLSAKHTLFAAGDSSVVPDGWKTTHLLFSLGAVCLDSRVGLDWACSMAEILRSLSGAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGIPQVLCVGTRCVYVDNANEPHDVIILKHFTEKNRAVVVDVKTRKRKTVKDYQLVQKGQECNDCQAQLSQYSQHFAFIASHLLQTSMESHCPEAVEATWVLSLALKGLYKTLKAHGFEETHATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEIHTSVEHTGLEPDERGDQGEDTEGAVSSPGEQEQRKLDPLESLDEPTRICFLMAHDALNAPLHVLRAIYELQMKRTDSFFLEVQKRFDGDELTTDERIRTLAQRWQPSRSLRLDEQSAKAVDTDMIILPCLSRPARCDQATAESNPVTQKLISSTEGELQQSYAQQRRSKSAALLHKELGCKSRRAVREYLFRVNEATAVLYARHVLASLLAEWPGHVPVSEETLELSGPAHMTYILDMFMQLEEKHQWEKILRKVLQGCRGSMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGAIYLSIKFDAQCSTEEGCDELAMSSSSDFQQDRHTFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYKFTVTAGHLGRFQTGFEILKQMLSEERVVPHLPLAQIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCTHSDLCDLTLLKPLWQLFTHMEYSLFEDVTQPGILLPLHRALTELFLVTENRAQELGLLQEYLLALTTDDHLLRCAAQALQNIAAISLAINYPNKATRLWNVKC

Target ORF information:

RefSeq Version	XM_011288812.2
Organism	Felis catus(domestic cat)
Definition	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011288812.2

ORF Insert Sequence:

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAAGA ATGTCACCGA GCAAGAGCAC AAGCAGTCTC TGCAGCTCAC CTTCCGGTCA 
CTGTGCACGT ATTTTAGTGA TAAGGATCCA GGTGGCCTTC TGCTCTTGCC CGAGAAGGGT 
GACGTGGCCA GCATGAACAG CAGTGACGTG CTGGCGGTCA TGAGCGTCCT CCTGTCCGTC 
GCTGCTCGAG AGTGTGAGCT GTTGGTGCTC AGTGGGGCCC AAGGGGAGGC GGGCTCTGTG 
CTCTTCTCCC TGTTCTGGGC GGTCCAAGGC AGCCTGCTGT CCTGGTGCTA CCTGCAGCTG 
AAGAGCACGC ACTCCGGAGC CCGAGAGCTC GCCGCGGACC TCCTTGACCA GTATGTGGCA 
CAGTTTCTGG CGAGCTTGAG AGTGATTTTG GAATCCCTTT TGTCACAGTA TAGTGGAAAG 
ACCCTTGTAG AGAAATTGTG TAATTCCGTG TTTTCGATGG CAGCTCGTCA ACTGGTCATC 
TTTCTCCTGG ACTTCTGCAC TCTGGACATT TCGCACTGTG TGCTCCTGAG AGAGTTCAGC 
ACGCTCCCGG AATTGTTGAA GCAGCTGTGC GGTGACCCTG AAGGAGGGCC AAATACGCTC 
GATGTGGAGC CTTGGCCACA GGAGCGTCCG GTGGTGTTAC ACACGTGGAC GAAGGAGTCT 
GCACACAACT ATGAGAACAA CTGCCACGAG GTGTCCGTCT TTGTTAGCCC GGGCGCAACC 
TGTTTTGAGG TGGAATTTGA TGAGCGGTGT GAGACTGAGA AGCGGTACGA TTATCTGGAA 
TTTACCGACG CTAGAGGTGG AAAGACACGC TATGACACCA AAGTCGGCAC TGACAAGTGG 
CCCAAGAAAG TGACCTTTAA GGCCGGCCCG CGGCTCCAGT TCCTCTTTCA CTCTGACAGC 
AGTAACAATG AGTGGGGCTA CAAGTTCACA GTCACGGCCT ACGGTCTGCC CGACGTGGCC 
GTGTCGTGGG CGTCGGATTT ACAACTGCTT GTTTCCCGGC TGCTGGGACG CCTCGCTGCC 
CAGTGCATGG CGCTCAAGTC TACGCACAAG TTAGGGGGCG ACGTGGCCGT GCCCCCGGCA 
AAAATGGCGG CGGTCCTGAA CTCCCCGCTG TGGAAACCGG TCTTCCGGCA TCAGCTGTGT 
CTGGAGCTGG GATTAGCAGC CAGCTGGCCC ACTAGCCCGC GCCAGGATAG TAAGGAGGTC 
ACGTGCATCC CCGAGGATCC CTGCCGCCGT CTCCTTCTTG AGTTTGTACA GGCAGACCCT 
GCCCAGAGCT GCTGTGGGCC ACTGTCAGAA CTGTGCAAGG GACTCATACA GGCATGTAGA 
AAACAGGCCC CAAAGACAGA TAGAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC 
ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GACTTATATG AGAAGCTGCA GAAATATGTA 
AAAAGTGGGG GCCACACAGC CCTGAGCGAG GAGTTTGCAC AGGTGTACTA TTTGGCAGAT 
GGGATTCGGA TATGGATGCT AGAGATGAAG CAGAAGTCCC TGATGAGCCT GGGGAGCGAG 
GCAGAAGGAA GGCATGGGTC CGAGGCTGCC GAGCTGAACC CCGAGAGCCT GGCGAAAGAG 
TGTATTCAGA AGAGTCTCCT GTTGCTAAGA TTTTTGCCCA TGGGAGTGAG TTCAGAAGAA 
AGCCGTGACA AGTTGGCGGC CACAGATGAC GTGGATCACC TGCAGCCACG CGACAGGCGC 
CCGAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGCTGTCTCC CGTGGAAGCG 
GCCACACCTG CCGTGAGGGA CGGGGGTCCC GGCTCGGAAG CGCAGCCACA CCTGCCCCCC 
GGCAGCGGCC CCCCTGCCTC AGAGGTGCCC CCTGCCGCCC CTGAGGAGCC CTCGTCACCT 
TCCGCTCCCA CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TCCGGCTGCT CTCCTTCCGG 
TCAGTGGAGG AGGCCAGACC GGCGCCCACA GTGAAAGAGA AATACCCCCT GCTGAGGGAT 
GTCCTGGACT TCATCAAGGA CCAGTCCCTC TCCCACGAGA GTGTTGTGAA GGTTCTCTCC 
TGGAGGAAAG CCCAAGCGCA GAGCGTCCTC GAAGTCCTGA GGATAGTTCA GTACTGCGCG 
GAGTCCCTCG GGCAGCCGCA CTGCTTCCAG CCGCCGTGCA TTCTCTTCCT GCTGGAACTT 
CTGACCTGCC AGAAAGAGTT TACCAATTAT TTTGGGCACT TGGAGGGCTG TGGCGCAGAC 
CTGCACAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT CAAAGCGGTT 
AAAGGATTCA GCAGCATAAA CGACAGGTCC CTGCTGCCTG CCTTGTCCTG TGTGCAGACA 
GCCCTGCTTC ATCTTCTGGA CATGGGCTGG GAACCTGGCG ACCTCGCTTT CTTTGTGGAC 
ATTCAGCTAC CAGATCTCCT CATGAAAATG ACACAAGAGA ACATAAGTGT CCACGACAGT 
GTGATCAGCC AGTGGAGTGA AGACGATGAG CTTGCTGATG CCAAGCAGAA CTCAGAATGG 
ATGGACGAGT GTCAGGATGG CATGTTTGAG GCCTGGTATG AAAAAATAGC CCAGGAAGAC 
CCAGAGAAGC AGAGGAAAAT GCACATGTTC ATTGCCCGCT ACTCTGAGCT GCTGCACGTG 
GACATCTCCT GCGACGGGTG TGACGAGATC GCGCCCTGGC ACCGCTACCG GTGTCTGCAG 
TGCAGCGACA TGGATCTCTG CACGACCTGC TTCCTAGGTG GGGTGAAGCC CGAGGGCCAC 
GGAGATGACC ACGAGATGGT CAGCATGGAG TTTACCTGTG ACCAGTGCCA GGGTTTGATC 
ATCGGCCGGA GGATGAATTG CAATGTGTGT GATGACTTCG ATCTTTGCTA TGGGTGCTAT 
GCGGCGAAGA AATACTCCTA TGGCCATCTG CCCACCCACA GTGTCACGGC TCATCCGATG 
GTGACCATCC GGATCAGCGA CCGGCAGAGG CTCATCCAGC CTTACATCCA CAACTACGCC 
TGGCTGCTCT TCGCCGCGCT GGCTCTCTAT AGCACCCACC TGGCCAGCGC AGAGGATGCG 
GGCGGTGAGA GGCTGGATTC CCGGGCCCGC AGCAGCGCCG CGGCCCTGCA CAGCCAGTGC 
ATGCAGCTTG TGGGGGACTG TCTGATGAAG GCTCACCAGG GAAAAGGTCT GAAAGCCCTA 
GCTCTTCTCG GGGTACTGCC AGATGGTGAC GCTAGTCCTC AAAGTCAGGC CCCGGCCGTC 
GCTGTGCCCG CCCACACACC GGAGGAGGAG AGAGCGGTCC GGGTCGCTGA GGGCCCGTCA 
CACACTGGCC CTTCTCCGCA CGACACTGGA AAGAGAGATG GTCACAAGGT CAGACCTTTC 
GACTGCAAGC AGAGGAGTAA GGAGGACGAG GGTGCATCTT TAATGAAGGA TCCCGCACGC 
CAAACTCAAG TTTCAGACTC ACCTGCAGGG CTTCCAGATG CTGACACTTC AGTCGTGTCC 
TCTCAGAAGC CCATCGAGGA AAAGGCAGTT ACTCCCAGCC CGGAGCAGGT GTTTGCTGAG 
TGTTCCCAGA AGAGGATTTT GGGATTACTA GCAGCCATGT TACCTCCCTT AAAGTTGGAC 
CCCGCGGTCC CCGTGATAGA CCTGGAGCGT GCCCTCCCAC TCATGTTTCA GGTTGTCATC 
TCGAACGCAG GCCACCTGAA CGAAACCTAC CACCTCACGC TGGGTCTTCT CGGCCAGTTA 
ATCACCCGCC TTTTACCAGC AGAGGTGGAT GCTGCCGTGG TGAAGGTCCT CTCCGCCAAG 
CACACCTTGT TTGCAGCGGG GGACAGTTCC GTCGTGCCAG ATGGCTGGAA GACCACCCAC 
CTGCTCTTCA GCCTGGGAGC CGTGTGTCTG GACAGCCGGG TGGGCTTGGA CTGGGCCTGC 
TCCATGGCGG AGATCCTGCG GTCCCTCAGC GGTGCCCCCC TGTGGCGTGA CGTCATCGCC 
ACCTTCACGG ACCACTGCAT CAAGCAGCTG CCGTTCCAGC TGAAGCACAC CAACATCTTC 
ACCCTGCTTG TGCTGGTTGG CATCCCCCAG GTCCTCTGTG TGGGAACTCG CTGTGTGTAT 
GTGGATAATG CCAATGAGCC CCATGACGTG ATCATCCTGA AGCACTTCAC TGAGAAGAAC 
AGGGCTGTCG TCGTCGATGT CAAAACCCGG AAAAGGAAAA CAGTGAAGGA CTACCAGCTG 
GTCCAGAAAG GACAGGAATG CAACGACTGT CAGGCCCAGC TGAGCCAGTA CTCCCAGCAC 
TTTGCCTTTA TCGCCAGTCA CCTCCTGCAA ACCAGCATGG AGAGCCATTG TCCTGAGGCA 
GTGGAGGCAA CTTGGGTCCT GTCCCTGGCC CTCAAAGGAC TGTATAAGAC ACTGAAGGCT 
CACGGTTTCG AAGAGACCCA TGCGACCTTC CTTCAGACGG ATTTGCTGAA GCTGCTGGTG 
AAAAAGTGCA GCAAAGGAAC TGGTTTCAGT AAAACGTGGC TCCTCCGGGA TCTAGAGATT 
TTGTCCATCA TGCTGTACTC CTCAAAGAAG GAGATCCACA CCTCGGTCGA GCACACAGGC 
CTGGAACCGG ATGAGCGAGG GGATCAAGGG GAGGACACAG AAGGCGCGGT CAGCAGCCCT 
GGAGAGCAGG AGCAGAGGAA GCTAGATCCT CTGGAAAGCC TGGATGAGCC CACCAGAATA 
TGTTTCTTGA TGGCGCACGA CGCCCTCAAT GCCCCTCTGC ACGTTCTCCG GGCCATATAC 
GAGCTGCAGA TGAAGAGGAC CGATTCTTTC TTCCTGGAGG TTCAGAAGAG GTTTGATGGG 
GATGAGCTCA CCACGGACGA AAGGATCCGG ACCCTGGCTC AGAGGTGGCA GCCCAGTAGG 
AGTTTGAGAC TGGACGAGCA GAGCGCCAAG GCCGTGGACA CAGACATGAT TATCTTACCG 
TGTCTGTCGC GGCCCGCACG CTGTGACCAG GCCACTGCCG AGTCCAACCC CGTGACCCAG 
AAGCTGATCT CCAGCACGGA GGGCGAACTG CAGCAGAGCT ATGCCCAGCA GCGCCGGAGC 
AAGAGTGCTG CCCTCCTGCA CAAGGAGTTG GGCTGCAAGA GCAGGAGGGC CGTCCGCGAG 
TACCTCTTCC GCGTGAATGA GGCCACGGCT GTCCTGTATG CCCGCCACGT GCTGGCGTCC 
TTGCTCGCTG AGTGGCCTGG GCACGTGCCC GTGAGCGAGG AGACCCTGGA GCTGAGCGGC 
CCGGCCCACA TGACCTACAT TTTGGATATG TTCATGCAGC TGGAAGAAAA GCATCAGTGG 
GAGAAGATCC TGCGGAAGGT ACTCCAGGGC TGCCGAGGGA GCATGCTGGG GACCATGGCC 
CTGGCTGCCT GCCAGTTCAT GGAGGAGCCT GGGATGGAAG TGCAAGTGAG GGAGTCCAAA 
CACCCGTATA ACAATAACAC CAACTTTGAG GATAAGGTCC ACATCCCTGG TGCAATCTAC 
CTCTCAATCA AATTCGACGC GCAGTGCAGC ACAGAGGAGG GCTGTGACGA GCTGGCCATG 
TCCAGCAGCA GTGACTTCCA GCAGGATCGA CACACTTTCA GTGGGTCTCA GCAGAAATGG 
AAGGATTTTG AGCTTCCAGG AGATACTCTG TATTATCGCT TCACCTCTGA CATGAGCAAC 
ACGGAGTGGG GCTACAAATT CACTGTGACG GCTGGACACC TCGGGCGATT CCAGACAGGG 
TTCGAGATTT TGAAGCAGAT GTTGTCAGAA GAAAGAGTTG TGCCACACCT CCCACTGGCC 
CAGATTTGGG AATGGCTGGT GGGTGTGGCT TGTCGCCAGA CTGGCCATCA GCGATTGAAA 
GCCATTCACC TGCTTCTGAG GATCGTCCGC TGCTGCACCC ACAGCGACCT CTGTGACCTG 
ACGCTGCTAA AGCCCCTGTG GCAGCTCTTC ACGCACATGG AGTACAGCCT GTTTGAGGAC 
GTGACACAGC CTGGCATCCT CCTCCCCCTG CACCGTGCCC TCACGGAGCT CTTCCTTGTC 
ACCGAGAACC GCGCCCAGGA GCTGGGGTTG CTGCAGGAGT ACCTGCTGGC CTTAACCACC 
GACGACCACC TTCTCCGCTG CGCGGCACAG GCTCTACAGA ACATCGCTGC TATCAGCCTG 
GCCATCAACT ACCCAAACAA GGCAACCCGC CTCTGGAATG TCAAGTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZZEF1 ( XM_023243635.1 ) cDNA ORF clone, Felis catus(domestic cat) -> XP_023099403.1 Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X4, mRNA.

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CloneID	OFb64466
Clone ID Related Accession (Same CDS sequence)	XM_023243635.1
Accession Version	XM_023243635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018736.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAATG TCACCGAGCA AGAGCACAAG CAGTCTCTGC AGCTCACCTT CCGGTCACTG 
TGCACGTATT TTAGTGATAA GGATCCAGGT GGCCTTCTGC TCTTGCCCGA GAAGGGTGAC 
GTGGCCAGCA TGAACAGCAG TGACGTGCTG GCGGTCATGA GCGTCCTCCT GTCCGTCGCT 
GCTCGAGAGT GTGAGCTGTT GGTGCTCAGT GGGGCCCAAG GGGAGGCGGG CTCTGTGCTC 
TTCTCCCTGT TCTGGGCGGT CCAAGGCAGC CTGCTGTCCT GGTGCTACCT GCAGCTGAAG 
AGCACGCACT CCGGAGCCCG AGAGCTCGCC GCGGACCTCC TTGACCAGTA TGTGGCACAG 
TTTCTGGCGA GCTTGAGAGT GATTTTGGAA TCCCTTTTGT CACAGTATAG TGGAAAGACC 
CTTGTAGAGA AATTGTGTAA TTCCGTGTTT TCGATGGCAG CTCGTCAACT GGTCATCTTT 
CTCCTGGACT TCTGCACTCT GGACATTTCG CACTGTGTGC TCCTGAGAGA GTTCAGCACG 
CTCCCGGAAT TGTTGAAGCA GCTGTGCGGT GACCCTGAAG GAGGGCCAAA TACGCTCGAT 
GTGGAGCCTT GGCCACAGGA GCGTCCGGTG GTGTTACACA CGTGGACGAA GGAGTCTGCA 
CACAACTATG AGAACAACTG CCACGAGGTG TCCGTCTTTG TTAGCCCGGG CGCAACCTGT 
TTTGAGGTGG AATTTGATGA GCGGTGTGAG ACTGAGAAGC GGTACGATTA TCTGGAATTT 
ACCGACGCTA GAGGTGGAAA GACACGCTAT GACACCAAAG TCGGCACTGA CAAGTGGCCC 
AAGAAAGTGA CCTTTAAGGC CGGCCCGCGG CTCCAGTTCC TCTTTCACTC TGACAGCAGT 
AACAATGAGT GGGGCTACAA GTTCACAGTC ACGGCCTACG GTCTGCCCGA CGTGGCCGTG 
TCGTGGGCGT CGGATTTACA ACTGCTTGTT TCCCGGCTGC TGGGACGCCT CGCTGCCCAG 
TGCATGGCGC TCAAGTCTAC GCACAAGTTA GGGGGCGACG TGGCCGTGCC CCCGGCAAAA 
ATGGCGGCGG TCCTGAACTC CCCGCTGTGG AAACCGGTCT TCCGGCATCA GCTGTGTCTG 
GAGCTGGGAT TAGCAGCCAG CTGGCCCACT AGCCCGCGCC AGGATAGTAA GGAGGTCACG 
TGCATCCCCG AGGATCCCTG CCGCCGTCTC CTTCTTGAGT TTGTACAGGC AGACCCTGCC 
CAGAGCTGCT GTGGGCCACT GTCAGAACTG TGCAAGGGAC TCATACAGGC ATGTAGAAAA 
CAGGCCCCAA AGACAGATAG AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC 
TTTGCTGCTC TGGTGTGTCG TACTCCAGAC TTATATGAGA AGCTGCAGAA ATATGTAAAA 
AGTGGGGGCC ACACAGCCCT GAGCGAGGAG TTTGCACAGG TGTACTATTT GGCAGATGGG 
ATTCGGATAT GGATGCTAGA GATGAAGCAG AAGTCCCTGA TGAGCCTGGG GAGCGAGGCA 
GAAGGAAGGC ATGGGTCCGA GGCTGCCGAG CTGAACCCCG AGAGCCTGGC GAAAGAGTGT 
ATTCAGAAGA GTCTCCTGTT GCTAAGATTT TTGCCCATGG GAGTGAGTTC AGAAGAAAGC 
CGTGACAAGT TGGCGGCCAC AGATGACGTG GATCACCTGC AGCCACGCGA CAGGCGCCCG 
AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGC TGTCTCCCGT GGAAGCGGCC 
ACACCTGCCG TGAGGGACGG GGGTCCCGGC TCGGAAGCGC AGCCACACCT GCCCCCCGGC 
AGCGGCCCCC CTGCCTCAGA GGTGCCCCCT GCCGCCCCTG AGGAGCCCTC GTCACCTTCC 
GCTCCCACCC GGCGGCCTCC CTTCACCCGA GGGCGACTCC GGCTGCTCTC CTTCCGGTCA 
GTGGAGGAGG CCAGACCGGC GCCCACAGTG AAAGAGAAAT ACCCCCTGCT GAGGGATGTC 
CTGGACTTCA TCAAGGACCA GTCCCTCTCC CACGAGAGTG TTGTGAAGGT TCTCTCCTGG 
AGGAAAGCCC AAGCGCAGAG CGTCCTCGAA GTCCTGAGGA TAGTTCAGTA CTGCGCGGAG 
TCCCTCGGGC AGCCGCACTG CTTCCAGCCG CCGTGCATTC TCTTCCTGCT GGAACTTCTG 
ACCTGCCAGA AAGAGTTTAC CAATTATTTT GGGCACTTGG AGGGCTGTGG CGCAGACCTG 
CACAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTCAA AGCGGTTAAA 
GGATTCAGCA GCATAAACGA CAGGTCCCTG CTGCCTGCCT TGTCCTGTGT GCAGACAGCC 
CTGCTTCATC TTCTGGACAT GGGCTGGGAA CCTGGCGACC TCGCTTTCTT TGTGGACATT 
CAGCTACCAG ATCTCCTCAT GAAAATGACA CAAGAGAACA TAAGTGTCCA CGACAGTGTG 
ATCAGCCAGT GGAGTGAAGA CGATGAGCTT GCTGATGCCA AGCAGAACTC AGAATGGATG 
GACGAGTGTC AGGATGGCAT GTTTGAGGCC TGGTATGAAA AAATAGCCCA GGAAGACCCA 
GAGAAGCAGA GGAAAATGCA CATGTTCATT GCCCGCTACT CTGAGCTGCT GCACGTGGAC 
ATCTCCTGCG ACGGGTGTGA CGAGATCGCG CCCTGGCACC GCTACCGGTG TCTGCAGTGC 
AGCGACATGG ATCTCTGCAC GACCTGCTTC CTAGGTGGGG TGAAGCCCGA GGGCCACGGA 
GATGACCACG AGATGGTCAG CATGGAGTTT ACCTGTGACC AGTGCCAGGG TTTGATCATC 
GGCCGGAGGA TGAATTGCAA TGTGTGTGAT GACTTCGATC TTTGCTATGG GTGCTATGCG 
GCGAAGAAAT ACTCCTATGG CCATCTGCCC ACCCACAGTG TCACGGCTCA TCCGATGGTG 
ACCATCCGGA TCAGCGACCG GCAGAGGCTC ATCCAGCCTT ACATCCACAA CTACGCCTGG 
CTGCTCTTCG CCGCGCTGGC TCTCTATAGC ACCCACCTGG CCAGCGCAGA GGATGCGGGC 
GGTGAGAGGC TGGATTCCCG GGCCCGCAGC AGCGCCGCGG CCCTGCACAG CCAGTGCATG 
CAGCTTGTGG GGGACTGTCT GATGAAGGCT CACCAGGGAA AAGGTCTGAA AGCCCTAGCT 
CTTCTCGGGG TACTGCCAGA TGGTGACGCT AGTCCTCAAA GTCAGGCCCC GGCCGTCGCT 
GTGCCCGCCC ACACACCGGA GGAGGAGAGA GCGGTCCGGG TCGCTGAGGG CCCGTCACAC 
ACTGGCCCTT CTCCGCACGA CACTGGAAAG AGAGATGGTC ACAAGGTCAG ACCTTTCGAC 
TGCAAGCAGA GGAGTAAGGA GGACGAGGGT GCATCTTTAA TGAAGGATCC CGCACGCCAA 
ACTCAAGTTT CAGACTCACC TGCAGGGCTT CCAGATGCTG ACACTTCAGT CGTGTCCTCT 
CAGAAGCCCA TCGAGGAAAA GGCAGTTACT CCCAGCCCGG AGCAGGTGTT TGCTGAGTGT 
TCCCAGAAGA GGATTTTGGG ATTACTAGCA GCCATGTTAC CTCCCTTAAA GTTGGACCCC 
GCGGTCCCCG TGATAGACCT GGAGCGTGCC CTCCCACTCA TGTTTCAGGT TGTCATCTCG 
AACGCAGGCC ACCTGAACGA AACCTACCAC CTCACGCTGG GTCTTCTCGG CCAGTTAATC 
ACCCGCCTTT TACCAGCAGA GGTGGATGCT GCCGTGGTGA AGGTCCTCTC CGCCAAGCAC 
ACCTTGTTTG CAGCGGGGGA CAGTTCCGTC GTGCCAGATG GCTGGAAGAC CACCCACCTG 
CTCTTCAGCC TGGGAGCCGT GTGTCTGGAC AGCCGGGTGG GCTTGGACTG GGCCTGCTCC 
ATGGCGGAGA TCCTGCGGTC CCTCAGCGGT GCCCCCCTGT GGCGTGACGT CATCGCCACC 
TTCACGGACC ACTGCATCAA GCAGCTGCCG TTCCAGCTGA AGCACACCAA CATCTTCACC 
CTGCTTGTGC TGGTTGGCAT CCCCCAGGTC CTCTGTGTGG GAACTCGCTG TGTGTATGTG 
GATAATGCCA ATGAGCCCCA TGACGTGATC ATCCTGAAGC ACTTCACTGA GAAGAACAGG 
GCTGTCGTCG TCGATGTCAA AACCCGGAAA AGGAAAACAG TGAAGGACTA CCAGCTGGTC 
CAGAAAGGAC AGGAATGCAA CGACTGTCAG GCCCAGCTGA GCCAGTACTC CCAGCACTTT 
GCCTTTATCG CCAGTCACCT CCTGCAAACC AGCATGGAGA GCCATTGTCC TGAGGCAGTG 
GAGGCAACTT GGGTCCTGTC CCTGGCCCTC AAAGGACTGT ATAAGACACT GAAGGCTCAC 
GGTTTCGAAG AGACCCATGC GACCTTCCTT CAGACGGATT TGCTGAAGCT GCTGGTGAAA 
AAGTGCAGCA AAGGAACTGG TTTCAGTAAA ACGTGGCTCC TCCGGGATCT AGAGATTTTG 
TCCATCATGC TGTACTCCTC AAAGAAGGAG ATCCACACCT CGGTCGAGCA CACAGGCCTG 
GAACCGGATG AGCGAGGGGA TCAAGGGGAG GACACAGAAG GCGCGGTCAG CAGCCCTGGA 
GAGCAGGAGC AGAGGAAGCT AGATCCTCTG GAAAGCCTGG ATGAGCCCAC CAGAATATGT 
TTCTTGATGG CGCACGACGC CCTCAATGCC CCTCTGCACG TTCTCCGGGC CATATACGAG 
CTGCAGATGA AGAGGACCGA TTCTTTCTTC CTGGAGGTTC AGAAGAGGTT TGATGGGGAT 
GAGCTCACCA CGGACGAAAG GATCCGGACC CTGGCTCAGA GGTGGCAGCC CAGTAGGAGT 
TTGAGACTGG ACGAGCAGAG CGCCAAGGCC GTGGACACAG ACATGATTAT CTTACCGTGT 
CTGTCGCGGC CCGCACGCTG TGACCAGGCC ACTGCCGAGT CCAACCCCGT GACCCAGAAG 
CTGATCTCCA GCACGGAGGG CGAACTGCAG CAGAGCTATG CCCAGCAGCG CCGGAGCAAG 
AGTGCTGCCC TCCTGCACAA GGAGTTGGGC TGCAAGAGCA GGAGGGCCGT CCGCGAGTAC 
CTCTTCCGCG TGAATGAGGC CACGGCTGTC CTGTATGCCC GCCACGTGCT GGCGTCCTTG 
CTCGCTGAGT GGCCTGGGCA CGTGCCCGTG AGCGAGGAGA CCCTGGAGCT GAGCGGCCCG 
GCCCACATGA CCTACATTTT GGATATGTTC ATGCAGCTGG AAGAAAAGCA TCAGTGGGAG 
AAGATCCTGC GGAAGGTACT CCAGGGCTGC CGAGGGAGCA TGCTGGGGAC CATGGCCCTG 
GCTGCCTGCC AGTTCATGGA GGAGCCTGGG ATGGAAGTGC AAGTGAGGGA GTCCAAACAC 
CCGTATAACA ATAACACCAA CTTTGAGGAT AAGGTCCACA TCCCTGGTGC AATCTACCTC 
TCAATCAAAT TCGACGCGCA GTGCAGCACA GAGGAGGGCT GTGACGAGCT GGCCATGTCC 
AGCAGCAGTG ACTTCCAGCA GGATCGACAC ACTTTCAGTG GGTCTCAGCA GAAATGGAAG 
GATTTTGAGC TTCCAGGAGA TACTCTGTAT TATCGCTTCA CCTCTGACAT GAGCAACACG 
GAGTGGGGCT ACAAATTCAC TGTGACGGCT GGACACCTCG GGCGATTCCA GACAGGGTTC 
GAGATTTTGA AGCAGATGTT GTCAGAAGAA AGAGTTGTGC CACACCTCCC ACTGGCCCAG 
ATTTGGGAAT GGCTGGTGGG TGTGGCTTGT CGCCAGACTG GCCATCAGCG ATTGAAAGCC 
ATTCACCTGC TTCTGAGGAT CGTCCGCTGC TGCACCCACA GCGACCTCTG TGACCTGACG 
CTGCTAAAGC CCCTGTGGCA GCTCTTCACG CACATGGAGT ACAGCCTGTT TGAGGACGTG 
ACACAGCCTG GCATCCTCCT CCCCCTGCAC CGTGCCCTCA CGGAGCTCTT CCTTGTCACC 
GAGAACCGCG CCCAGGAGCT GGGGTTGCTG CAGGAGTACC TGCTGGCCTT AACCACCGAC 
GACCACCTTC TCCGCTGCGC GGCACAGGCT CTACAGAACA TCGCTGCTAT CAGCCTGGCC 
ATCAACTACC CAAACAAGGC AACCCGCCTC TGGAATGTCA AGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023099403.1
CDS	203..9070
Translation	MGNAPSHSSEDEAAAAGGEGWGPHQEWAADSGTIPGPGPAAPALPPAAALLEPARLREAAAALRPAPPCESLVSRHHGALLRWLEERLGRGEESVTLEQFRELLEARGAGCSGEQFEEAFAQFDAEGDGTVDAENMLEALKNSSGANLQGELGHIIRQLQACSLVPGFIDIFSESKEGLGVHASLILRFLHRNRLSGMAVPCPMLDHCNNMCSMRVSVLKESLDQLVQKEKESPGDLTRSPEMDKLKSVAKCYAYIETSSNPADIDKMTNGDTASYWQSDGSARSHWIRLKMKPDVVLRHLSIAVAATDQSYMPQQVTVAVGRSVGELQDVRDVHIPSNVTGYVTLLENANISQLYVQINIKRCLSDGCDTRIHGLRAVGFQRVKKSGVSVSDASAIWYWSLLTSLVTASMETNPAFVQTVLHNTQKALQHMPPLSLSPGSTDFSTFLSPNVLEEVDSFLIRITSCCSTPEVELTLLAFALARGSVAKVLSSLCAITDHLDTRYEASSLISSMASVRQNLLLKYGKPLQLILQACDVKGKEEKSGPENLLVEPWTRDGFLTETGKTRASTIFSTGTESAFQVTQIRIMVRRGGIGAQCGLVFAYNSSSEKFHAEEHFKRFEKYDKWKLQEFRQFVKSRAGCSPGDLGEEDPVGWFELEEEWDEADVKLQQCRVAQYLMVKFLCTRQESAERLGVQGLSISGHLRPARTGAEQHVVCTRCVKDTEDSVCGATLLLRTLQFIQQLAHDLVQQKESGLKYKSFLDFAGLDLQIFWNFYSKLKQNPREECICAQTLLLRLLQSCFSVLRSDGPAASQDAKAPRQSPAGAGAAKELHTHLCDVVDRLDGDSVPMETLKQEVKNTLLNGAAIFFPDRQTRRDHLFAMMNVTEQEHKQSLQLTFRSLCTYFSDKDPGGLLLLPEKGDVASMNSSDVLAVMSVLLSVAARECELLVLSGAQGEAGSVLFSLFWAVQGSLLSWCYLQLKSTHSGARELAADLLDQYVAQFLASLRVILESLLSQYSGKTLVEKLCNSVFSMAARQLVIFLLDFCTLDISHCVLLREFSTLPELLKQLCGDPEGGPNTLDVEPWPQERPVVLHTWTKESAHNYENNCHEVSVFVSPGATCFEVEFDERCETEKRYDYLEFTDARGGKTRYDTKVGTDKWPKKVTFKAGPRLQFLFHSDSSNNEWGYKFTVTAYGLPDVAVSWASDLQLLVSRLLGRLAAQCMALKSTHKLGGDVAVPPAKMAAVLNSPLWKPVFRHQLCLELGLAASWPTSPRQDSKEVTCIPEDPCRRLLLEFVQADPAQSCCGPLSELCKGLIQACRKQAPKTDRVAGSTIDQAVNATFAALVCRTPDLYEKLQKYVKSGGHTALSEEFAQVYYLADGIRIWMLEMKQKSLMSLGSEAEGRHGSEAAELNPESLAKECIQKSLLLLRFLPMGVSSEESRDKLAATDDVDHLQPRDRRPRTSSVVEEHFQASLSPVEAATPAVRDGGPGSEAQPHLPPGSGPPASEVPPAAPEEPSSPSAPTRRPPFTRGRLRLLSFRSVEEARPAPTVKEKYPLLRDVLDFIKDQSLSHESVVKVLSWRKAQAQSVLEVLRIVQYCAESLGQPHCFQPPCILFLLELLTCQKEFTNYFGHLEGCGADLHKEIRDTYYQFVLFLVKAVKGFSSINDRSLLPALSCVQTALLHLLDMGWEPGDLAFFVDIQLPDLLMKMTQENISVHDSVISQWSEDDELADAKQNSEWMDECQDGMFEAWYEKIAQEDPEKQRKMHMFIARYSELLHVDISCDGCDEIAPWHRYRCLQCSDMDLCTTCFLGGVKPEGHGDDHEMVSMEFTCDQCQGLIIGRRMNCNVCDDFDLCYGCYAAKKYSYGHLPTHSVTAHPMVTIRISDRQRLIQPYIHNYAWLLFAALALYSTHLASAEDAGGERLDSRARSSAAALHSQCMQLVGDCLMKAHQGKGLKALALLGVLPDGDASPQSQAPAVAVPAHTPEEERAVRVAEGPSHTGPSPHDTGKRDGHKVRPFDCKQRSKEDEGASLMKDPARQTQVSDSPAGLPDADTSVVSSQKPIEEKAVTPSPEQVFAECSQKRILGLLAAMLPPLKLDPAVPVIDLERALPLMFQVVISNAGHLNETYHLTLGLLGQLITRLLPAEVDAAVVKVLSAKHTLFAAGDSSVVPDGWKTTHLLFSLGAVCLDSRVGLDWACSMAEILRSLSGAPLWRDVIATFTDHCIKQLPFQLKHTNIFTLLVLVGIPQVLCVGTRCVYVDNANEPHDVIILKHFTEKNRAVVVDVKTRKRKTVKDYQLVQKGQECNDCQAQLSQYSQHFAFIASHLLQTSMESHCPEAVEATWVLSLALKGLYKTLKAHGFEETHATFLQTDLLKLLVKKCSKGTGFSKTWLLRDLEILSIMLYSSKKEIHTSVEHTGLEPDERGDQGEDTEGAVSSPGEQEQRKLDPLESLDEPTRICFLMAHDALNAPLHVLRAIYELQMKRTDSFFLEVQKRFDGDELTTDERIRTLAQRWQPSRSLRLDEQSAKAVDTDMIILPCLSRPARCDQATAESNPVTQKLISSTEGELQQSYAQQRRSKSAALLHKELGCKSRRAVREYLFRVNEATAVLYARHVLASLLAEWPGHVPVSEETLELSGPAHMTYILDMFMQLEEKHQWEKILRKVLQGCRGSMLGTMALAACQFMEEPGMEVQVRESKHPYNNNTNFEDKVHIPGAIYLSIKFDAQCSTEEGCDELAMSSSSDFQQDRHTFSGSQQKWKDFELPGDTLYYRFTSDMSNTEWGYKFTVTAGHLGRFQTGFEILKQMLSEERVVPHLPLAQIWEWLVGVACRQTGHQRLKAIHLLLRIVRCCTHSDLCDLTLLKPLWQLFTHMEYSLFEDVTQPGILLPLHRALTELFLVTENRAQELGLLQEYLLALTTDDHLLRCAAQALQNIAAISLAINYPNKATRLWNVKC

Target ORF information:

RefSeq Version	XM_023243635.1
Organism	Felis catus(domestic cat)
Definition	Felis catus zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023243635.1

ORF Insert Sequence:

ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCCG CCGCCGCCGG TGGCGAGGGC 
TGGGGCCCGC ACCAGGAGTG GGCCGCAGAC TCGGGCACGA TCCCCGGCCC GGGCCCCGCG 
GCGCCCGCGC TGCCGCCCGC CGCGGCGCTG CTGGAGCCGG CCCGGCTGCG GGAGGCGGCG 
GCGGCGTTGC GGCCCGCGCC GCCCTGCGAG TCTCTGGTGT CTAGGCACCA CGGCGCGCTG 
CTGCGCTGGC TGGAAGAGCG GCTGGGCCGC GGCGAAGAGT CCGTCACCTT GGAGCAGTTC 
CGGGAGCTGC TGGAGGCTCG TGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT 
GCCCAGTTTG ACGCCGAGGG TGATGGTACA GTGGATGCCG AGAACATGCT GGAGGCCCTC 
AAGAACTCCA GTGGGGCTAA CCTCCAGGGG GAGCTAGGCC ACATCATCAG GCAACTGCAG 
GCCTGCTCTC TGGTCCCAGG TTTTATAGAC ATATTTTCAG AGTCCAAGGA GGGCCTGGGT 
GTTCACGCGT CCCTGATCCT GCGTTTCCTG CATCGCAACC GGCTCTCCGG CATGGCCGTC 
CCCTGCCCCA TGTTGGACCA CTGCAATAAC ATGTGCTCCA TGAGGGTTTC GGTTCTGAAG 
GAGTCCCTGG ACCAGCTGGT ACAGAAGGAG AAGGAGAGCC CTGGAGACCT AACTAGGAGC 
CCTGAGATGG ATAAACTCAA GTCGGTGGCA AAGTGCTACG CTTACATAGA AACGTCCTCC 
AACCCCGCGG ACATCGACAA GATGACAAAT GGAGACACGG CGTCATACTG GCAGTCGGAC 
GGCAGTGCCC GCTCACACTG GATCCGCTTA AAAATGAAGC CAGATGTTGT GCTTAGACAC 
TTGTCCATCG CAGTGGCTGC CACAGACCAG AGCTACATGC CGCAGCAGGT GACCGTGGCT 
GTGGGGAGGA GTGTCGGTGA GCTCCAGGAT GTCCGCGACG TGCACATCCC CAGCAATGTC 
ACCGGCTACG TGACGCTGCT GGAAAATGCC AACATCAGCC AGCTCTATGT CCAGATTAAC 
ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC 
TTTCAGAGAG TTAAGAAGTC TGGGGTCTCA GTCTCAGATG CTTCTGCAAT CTGGTACTGG 
TCTCTGCTGA CATCTCTGGT GACGGCGTCC ATGGAGACAA ACCCCGCCTT CGTCCAGACC 
GTGCTGCACA ATACTCAGAA GGCGCTGCAG CACATGCCTC CACTTTCCCT CTCACCAGGA 
TCCACGGATT TCTCGACTTT CCTGTCTCCC AACGTTCTTG AAGAAGTGGA CAGCTTCCTC 
ATAAGAATAA CGAGCTGCTG TTCGACTCCA GAGGTGGAGC TGACCCTTCT GGCTTTTGCA 
CTAGCAAGAG GAAGTGTCGC CAAAGTGCTG AGCTCTCTGT GTGCCATCAC TGACCACCTG 
GACACACGCT ACGAAGCCTC GTCCCTCATC TCCTCCATGG CGTCAGTCAG GCAGAACCTG 
CTCCTTAAAT ACGGTAAACC TCTCCAGTTG ATCCTTCAGG CCTGTGATGT CAAAGGGAAG 
GAAGAGAAAT CAGGGCCTGA AAACCTCCTC GTCGAGCCGT GGACGAGAGA CGGGTTTCTT 
ACAGAGACTG GAAAAACCAG AGCAAGTACT ATTTTTTCTA CAGGAACTGA ATCTGCCTTC 
CAAGTTACAC AGATCAGAAT TATGGTTCGA CGTGGTGGCA TTGGGGCCCA GTGCGGGTTG 
GTGTTTGCCT ACAACTCATC TTCTGAGAAA TTTCACGCAG AAGAACACTT CAAAAGGTTT 
GAAAAGTATG ACAAATGGAA ACTTCAGGAA TTCAGGCAGT TTGTAAAAAG CAGGGCTGGT 
TGCTCACCTG GTGACCTTGG AGAGGAAGAC CCTGTTGGCT GGTTTGAGCT CGAAGAGGAA 
TGGGACGAAG CAGACGTGAA GCTGCAACAG TGCAGAGTCG CCCAATATTT GATGGTGAAG 
TTCCTCTGCA CCCGCCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGCCT CAGCATCAGT 
GGGCATCTCC GGCCGGCGAG AACCGGGGCA GAGCAGCACG TCGTCTGCAC CCGCTGCGTA 
AAGGACACGG AGGACAGCGT CTGCGGGGCC ACCCTGCTGC TCAGGACCCT TCAGTTCATT 
CAGCAGCTCG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA CAAATCTTTT 
CTGGACTTCG CGGGTCTTGA TTTACAGATC TTCTGGAATT TTTACAGTAA ATTAAAACAA 
AATCCGAGGG AAGAATGCAT CTGTGCCCAA ACCCTGCTTC TGCGCCTCCT GCAGAGCTGT 
TTCTCCGTGC TGCGGAGCGA CGGGCCGGCC GCCTCTCAGG ACGCAAAGGC TCCCCGGCAG 
AGCCCTGCAG GGGCGGGGGC CGCCAAGGAG CTCCACACGC ACTTGTGTGA CGTGGTGGAT 
AGGCTAGATG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAA GAACACCCTT 
CTTAATGGGG CTGCCATCTT CTTTCCCGAT CGACAGACCC GACGGGACCA CCTCTTTGCC 
ATGATGAATG TCACCGAGCA AGAGCACAAG CAGTCTCTGC AGCTCACCTT CCGGTCACTG 
TGCACGTATT TTAGTGATAA GGATCCAGGT GGCCTTCTGC TCTTGCCCGA GAAGGGTGAC 
GTGGCCAGCA TGAACAGCAG TGACGTGCTG GCGGTCATGA GCGTCCTCCT GTCCGTCGCT 
GCTCGAGAGT GTGAGCTGTT GGTGCTCAGT GGGGCCCAAG GGGAGGCGGG CTCTGTGCTC 
TTCTCCCTGT TCTGGGCGGT CCAAGGCAGC CTGCTGTCCT GGTGCTACCT GCAGCTGAAG 
AGCACGCACT CCGGAGCCCG AGAGCTCGCC GCGGACCTCC TTGACCAGTA TGTGGCACAG 
TTTCTGGCGA GCTTGAGAGT GATTTTGGAA TCCCTTTTGT CACAGTATAG TGGAAAGACC 
CTTGTAGAGA AATTGTGTAA TTCCGTGTTT TCGATGGCAG CTCGTCAACT GGTCATCTTT 
CTCCTGGACT TCTGCACTCT GGACATTTCG CACTGTGTGC TCCTGAGAGA GTTCAGCACG 
CTCCCGGAAT TGTTGAAGCA GCTGTGCGGT GACCCTGAAG GAGGGCCAAA TACGCTCGAT 
GTGGAGCCTT GGCCACAGGA GCGTCCGGTG GTGTTACACA CGTGGACGAA GGAGTCTGCA 
CACAACTATG AGAACAACTG CCACGAGGTG TCCGTCTTTG TTAGCCCGGG CGCAACCTGT 
TTTGAGGTGG AATTTGATGA GCGGTGTGAG ACTGAGAAGC GGTACGATTA TCTGGAATTT 
ACCGACGCTA GAGGTGGAAA GACACGCTAT GACACCAAAG TCGGCACTGA CAAGTGGCCC 
AAGAAAGTGA CCTTTAAGGC CGGCCCGCGG CTCCAGTTCC TCTTTCACTC TGACAGCAGT 
AACAATGAGT GGGGCTACAA GTTCACAGTC ACGGCCTACG GTCTGCCCGA CGTGGCCGTG 
TCGTGGGCGT CGGATTTACA ACTGCTTGTT TCCCGGCTGC TGGGACGCCT CGCTGCCCAG 
TGCATGGCGC TCAAGTCTAC GCACAAGTTA GGGGGCGACG TGGCCGTGCC CCCGGCAAAA 
ATGGCGGCGG TCCTGAACTC CCCGCTGTGG AAACCGGTCT TCCGGCATCA GCTGTGTCTG 
GAGCTGGGAT TAGCAGCCAG CTGGCCCACT AGCCCGCGCC AGGATAGTAA GGAGGTCACG 
TGCATCCCCG AGGATCCCTG CCGCCGTCTC CTTCTTGAGT TTGTACAGGC AGACCCTGCC 
CAGAGCTGCT GTGGGCCACT GTCAGAACTG TGCAAGGGAC TCATACAGGC ATGTAGAAAA 
CAGGCCCCAA AGACAGATAG AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC 
TTTGCTGCTC TGGTGTGTCG TACTCCAGAC TTATATGAGA AGCTGCAGAA ATATGTAAAA 
AGTGGGGGCC ACACAGCCCT GAGCGAGGAG TTTGCACAGG TGTACTATTT GGCAGATGGG 
ATTCGGATAT GGATGCTAGA GATGAAGCAG AAGTCCCTGA TGAGCCTGGG GAGCGAGGCA 
GAAGGAAGGC ATGGGTCCGA GGCTGCCGAG CTGAACCCCG AGAGCCTGGC GAAAGAGTGT 
ATTCAGAAGA GTCTCCTGTT GCTAAGATTT TTGCCCATGG GAGTGAGTTC AGAAGAAAGC 
CGTGACAAGT TGGCGGCCAC AGATGACGTG GATCACCTGC AGCCACGCGA CAGGCGCCCG 
AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGC TGTCTCCCGT GGAAGCGGCC 
ACACCTGCCG TGAGGGACGG GGGTCCCGGC TCGGAAGCGC AGCCACACCT GCCCCCCGGC 
AGCGGCCCCC CTGCCTCAGA GGTGCCCCCT GCCGCCCCTG AGGAGCCCTC GTCACCTTCC 
GCTCCCACCC GGCGGCCTCC CTTCACCCGA GGGCGACTCC GGCTGCTCTC CTTCCGGTCA 
GTGGAGGAGG CCAGACCGGC GCCCACAGTG AAAGAGAAAT ACCCCCTGCT GAGGGATGTC 
CTGGACTTCA TCAAGGACCA GTCCCTCTCC CACGAGAGTG TTGTGAAGGT TCTCTCCTGG 
AGGAAAGCCC AAGCGCAGAG CGTCCTCGAA GTCCTGAGGA TAGTTCAGTA CTGCGCGGAG 
TCCCTCGGGC AGCCGCACTG CTTCCAGCCG CCGTGCATTC TCTTCCTGCT GGAACTTCTG 
ACCTGCCAGA AAGAGTTTAC CAATTATTTT GGGCACTTGG AGGGCTGTGG CGCAGACCTG 
CACAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTCAA AGCGGTTAAA 
GGATTCAGCA GCATAAACGA CAGGTCCCTG CTGCCTGCCT TGTCCTGTGT GCAGACAGCC 
CTGCTTCATC TTCTGGACAT GGGCTGGGAA CCTGGCGACC TCGCTTTCTT TGTGGACATT 
CAGCTACCAG ATCTCCTCAT GAAAATGACA CAAGAGAACA TAAGTGTCCA CGACAGTGTG 
ATCAGCCAGT GGAGTGAAGA CGATGAGCTT GCTGATGCCA AGCAGAACTC AGAATGGATG 
GACGAGTGTC AGGATGGCAT GTTTGAGGCC TGGTATGAAA AAATAGCCCA GGAAGACCCA 
GAGAAGCAGA GGAAAATGCA CATGTTCATT GCCCGCTACT CTGAGCTGCT GCACGTGGAC 
ATCTCCTGCG ACGGGTGTGA CGAGATCGCG CCCTGGCACC GCTACCGGTG TCTGCAGTGC 
AGCGACATGG ATCTCTGCAC GACCTGCTTC CTAGGTGGGG TGAAGCCCGA GGGCCACGGA 
GATGACCACG AGATGGTCAG CATGGAGTTT ACCTGTGACC AGTGCCAGGG TTTGATCATC 
GGCCGGAGGA TGAATTGCAA TGTGTGTGAT GACTTCGATC TTTGCTATGG GTGCTATGCG 
GCGAAGAAAT ACTCCTATGG CCATCTGCCC ACCCACAGTG TCACGGCTCA TCCGATGGTG 
ACCATCCGGA TCAGCGACCG GCAGAGGCTC ATCCAGCCTT ACATCCACAA CTACGCCTGG 
CTGCTCTTCG CCGCGCTGGC TCTCTATAGC ACCCACCTGG CCAGCGCAGA GGATGCGGGC 
GGTGAGAGGC TGGATTCCCG GGCCCGCAGC AGCGCCGCGG CCCTGCACAG CCAGTGCATG 
CAGCTTGTGG GGGACTGTCT GATGAAGGCT CACCAGGGAA AAGGTCTGAA AGCCCTAGCT 
CTTCTCGGGG TACTGCCAGA TGGTGACGCT AGTCCTCAAA GTCAGGCCCC GGCCGTCGCT 
GTGCCCGCCC ACACACCGGA GGAGGAGAGA GCGGTCCGGG TCGCTGAGGG CCCGTCACAC 
ACTGGCCCTT CTCCGCACGA CACTGGAAAG AGAGATGGTC ACAAGGTCAG ACCTTTCGAC 
TGCAAGCAGA GGAGTAAGGA GGACGAGGGT GCATCTTTAA TGAAGGATCC CGCACGCCAA 
ACTCAAGTTT CAGACTCACC TGCAGGGCTT CCAGATGCTG ACACTTCAGT CGTGTCCTCT 
CAGAAGCCCA TCGAGGAAAA GGCAGTTACT CCCAGCCCGG AGCAGGTGTT TGCTGAGTGT 
TCCCAGAAGA GGATTTTGGG ATTACTAGCA GCCATGTTAC CTCCCTTAAA GTTGGACCCC 
GCGGTCCCCG TGATAGACCT GGAGCGTGCC CTCCCACTCA TGTTTCAGGT TGTCATCTCG 
AACGCAGGCC ACCTGAACGA AACCTACCAC CTCACGCTGG GTCTTCTCGG CCAGTTAATC 
ACCCGCCTTT TACCAGCAGA GGTGGATGCT GCCGTGGTGA AGGTCCTCTC CGCCAAGCAC 
ACCTTGTTTG CAGCGGGGGA CAGTTCCGTC GTGCCAGATG GCTGGAAGAC CACCCACCTG 
CTCTTCAGCC TGGGAGCCGT GTGTCTGGAC AGCCGGGTGG GCTTGGACTG GGCCTGCTCC 
ATGGCGGAGA TCCTGCGGTC CCTCAGCGGT GCCCCCCTGT GGCGTGACGT CATCGCCACC 
TTCACGGACC ACTGCATCAA GCAGCTGCCG TTCCAGCTGA AGCACACCAA CATCTTCACC 
CTGCTTGTGC TGGTTGGCAT CCCCCAGGTC CTCTGTGTGG GAACTCGCTG TGTGTATGTG 
GATAATGCCA ATGAGCCCCA TGACGTGATC ATCCTGAAGC ACTTCACTGA GAAGAACAGG 
GCTGTCGTCG TCGATGTCAA AACCCGGAAA AGGAAAACAG TGAAGGACTA CCAGCTGGTC 
CAGAAAGGAC AGGAATGCAA CGACTGTCAG GCCCAGCTGA GCCAGTACTC CCAGCACTTT 
GCCTTTATCG CCAGTCACCT CCTGCAAACC AGCATGGAGA GCCATTGTCC TGAGGCAGTG 
GAGGCAACTT GGGTCCTGTC CCTGGCCCTC AAAGGACTGT ATAAGACACT GAAGGCTCAC 
GGTTTCGAAG AGACCCATGC GACCTTCCTT CAGACGGATT TGCTGAAGCT GCTGGTGAAA 
AAGTGCAGCA AAGGAACTGG TTTCAGTAAA ACGTGGCTCC TCCGGGATCT AGAGATTTTG 
TCCATCATGC TGTACTCCTC AAAGAAGGAG ATCCACACCT CGGTCGAGCA CACAGGCCTG 
GAACCGGATG AGCGAGGGGA TCAAGGGGAG GACACAGAAG GCGCGGTCAG CAGCCCTGGA 
GAGCAGGAGC AGAGGAAGCT AGATCCTCTG GAAAGCCTGG ATGAGCCCAC CAGAATATGT 
TTCTTGATGG CGCACGACGC CCTCAATGCC CCTCTGCACG TTCTCCGGGC CATATACGAG 
CTGCAGATGA AGAGGACCGA TTCTTTCTTC CTGGAGGTTC AGAAGAGGTT TGATGGGGAT 
GAGCTCACCA CGGACGAAAG GATCCGGACC CTGGCTCAGA GGTGGCAGCC CAGTAGGAGT 
TTGAGACTGG ACGAGCAGAG CGCCAAGGCC GTGGACACAG ACATGATTAT CTTACCGTGT 
CTGTCGCGGC CCGCACGCTG TGACCAGGCC ACTGCCGAGT CCAACCCCGT GACCCAGAAG 
CTGATCTCCA GCACGGAGGG CGAACTGCAG CAGAGCTATG CCCAGCAGCG CCGGAGCAAG 
AGTGCTGCCC TCCTGCACAA GGAGTTGGGC TGCAAGAGCA GGAGGGCCGT CCGCGAGTAC 
CTCTTCCGCG TGAATGAGGC CACGGCTGTC CTGTATGCCC GCCACGTGCT GGCGTCCTTG 
CTCGCTGAGT GGCCTGGGCA CGTGCCCGTG AGCGAGGAGA CCCTGGAGCT GAGCGGCCCG 
GCCCACATGA CCTACATTTT GGATATGTTC ATGCAGCTGG AAGAAAAGCA TCAGTGGGAG 
AAGATCCTGC GGAAGGTACT CCAGGGCTGC CGAGGGAGCA TGCTGGGGAC CATGGCCCTG 
GCTGCCTGCC AGTTCATGGA GGAGCCTGGG ATGGAAGTGC AAGTGAGGGA GTCCAAACAC 
CCGTATAACA ATAACACCAA CTTTGAGGAT AAGGTCCACA TCCCTGGTGC AATCTACCTC 
TCAATCAAAT TCGACGCGCA GTGCAGCACA GAGGAGGGCT GTGACGAGCT GGCCATGTCC 
AGCAGCAGTG ACTTCCAGCA GGATCGACAC ACTTTCAGTG GGTCTCAGCA GAAATGGAAG 
GATTTTGAGC TTCCAGGAGA TACTCTGTAT TATCGCTTCA CCTCTGACAT GAGCAACACG 
GAGTGGGGCT ACAAATTCAC TGTGACGGCT GGACACCTCG GGCGATTCCA GACAGGGTTC 
GAGATTTTGA AGCAGATGTT GTCAGAAGAA AGAGTTGTGC CACACCTCCC ACTGGCCCAG 
ATTTGGGAAT GGCTGGTGGG TGTGGCTTGT CGCCAGACTG GCCATCAGCG ATTGAAAGCC 
ATTCACCTGC TTCTGAGGAT CGTCCGCTGC TGCACCCACA GCGACCTCTG TGACCTGACG 
CTGCTAAAGC CCCTGTGGCA GCTCTTCACG CACATGGAGT ACAGCCTGTT TGAGGACGTG 
ACACAGCCTG GCATCCTCCT CCCCCTGCAC CGTGCCCTCA CGGAGCTCTT CCTTGTCACC 
GAGAACCGCG CCCAGGAGCT GGGGTTGCTG CAGGAGTACC TGCTGGCCTT AACCACCGAC 
GACCACCTTC TCCGCTGCGC GGCACAGGCT CTACAGAACA TCGCTGCTAT CAGCCTGGCC 
ATCAACTACC CAAACAAGGC AACCCGCCTC TGGAATGTCA AGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ZZEF1 cDNA ORF clone, Felis catus(domestic cat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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