ESPL1 cDNA ORF clone, Felis catus(Domestic cat)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPL1
Entrez Gene ID	101084118
Full Name	extra spindle pole bodies like 1, separase
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003988757.3	XP_003988806.1	separin isoform X1
XM_003988757.4	XP_003988806.1	separin isoform X1
XM_019834989.1	XP_019690548.1	separin isoform X1
XM_019834990.2	XP_019690549.1	separin isoform X2
XM_019834990.1	XP_019690549.1	separin isoform X2

Pathways from BioSystems
Gene Ontology

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb45480	XM_019834990.2 Latest version!	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb09833	XM_003988757.4 Latest version!	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb09833	XM_019834989.1 Latest version!	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OFb09833	XM_003988757.3	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OFb45480	XM_019834990.1	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPL1 ( XM_019834990.2 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019690549.1 Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OFb45480
Clone ID Related Accession (Same CDS sequence)	XM_019834990.2 , XM_019834990.1
Accession Version	XM_019834990.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018729.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_019834990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGACCAGGAT CCTGATCACC AGTGTCTTCT CCTCAGTCGG GGGCCTCAGC 
AGTGCTAAGC CAAGGGGTGG ATCCACGCAA TACCTTCTAC GTCCTGAACC CTCACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019690549.1
CDS	175..6054
Translation	MRSFKGINFGTLLSSVKEAEELLPDLKEFLSKLPAGSNSCRSDAERRQACDAILRACNQQLTAKLACPRHLESLLELAELAYNGYLMSTPQRPPLYLERILFIFLRNIATQGIPEATLRFAKPLHACLVHCSRQAAPQDYDAVARGSFSLLWKAAETLEERRAALSTRLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDATGHSLNEADADFLDDLLSRHVIKVLVGEGGGSPGPLSPQRALCLLELTLERCRRLCWSGHHAKATRVVEKTHDYLRNTSLAPSLQLCQLGVELLQAGEGGSQALAELLIKASAVLNNSVEAPSPPLRALCDSCQFFLSGLERGIKRRYGLDAVLGLFAFLGGYCSLIRQLQDGVCGDSSKQQQSLVQMHFQGLHLYTVVVYDFAQSCQVADLADLAQLVESCKSTVVWMLEALQSLSGQELTDYLGMTASYTSNLAYSFYSHKLYSEACAISEPLCQHLGLVKPDMYPEVPPEKLHRCFRLHGESLKKLGKQAQGCEMVTLWLTALQPCGPQHMAEPVTFWVRIKMDAARAGDKELQLRTLRDSLSGWDPETLVFLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHDFAQQTNCSALDAIQEALQLLESVRPETQAKDRLLDDKAQALLWFYICTLETKMQEGIERDRRAQAPNNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKAQAPAVRCLQQTAASLQTLAALYQLAAKPLQALEVLLLVRIVSQRLEDHAKAAGSSCHASQLLLMLGCPSYAQLYLEEADSSLKLLDHNTDTYLLLSLTCNLLRSQLYCVHQKVTEGASLLLSVLRDPALQRSSKAWYLLRVQALQVVASYLSLSSDSLSASLREQLCAQGWQTPEIALVDSHKLLRSIILLLLGSDVLSIQKAAVETPFLDYGENLVQKWQVLTEVLSCSEKLVSRLGHLGCVSEAKAFCLDALKLTTKLQLPHQCALFLVLKAELELARTDIDLCESDLQQVLFLLESCTEFGGLAQHLDSVKKVHLQKGKQQTRVRRPPELPEEEPFLRGPALELVATVAKEPGPVAPSTNSSPVLKAKPQPSPGFLTHLPTCDCLLCASPVLSAVCLRWALVTAGVRLALGHQAQGLDLLQVVLKGCPAATERLTQALQTTLNHKAPPSPVPSLLDEILAQAYTQLALEGLSRPSHKSLGKVLESGLKFVAARVPHLEPWRASLLLIRALAKLAGLNCCTTQLFASSWGWQPPSVKPSPGSEPSKPRSQKLSGRGRQRAASSTQPLHNTSLKSLEGGGLPCTPKPPGQVRPAGTRVPFTVFEEVLPTKSKPEVPKAPRVQQRVQTRLKVNFSDDSDLEDPVSIEARPAEGPKRRGTASRGRGRGRGQARKNPSLKTVAVAASSSAPGLPGLGGRSRRAKKVTSGHCEELGSEIIRTIPEEEMTDNQMEMSFEILRGSDGEDSASGGKAPAPGPDTAIGECEVLRRDSSKEELPVPCPDKERDKDLGPRLRLSSAPVAIGLSTLDSICDLLTIAFRGVSHCPPSGLYAYLCRLLALCLGHRDPYATACLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEMADQVQRLSLQDRPGDVPLARIQHLFSFRASGSGQFPEPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQSKLPLSSALKEFDTIQKEQKENSSCTDKREWWTGRLELDHRMEALIISLEKHVLGCWRGLLLPSSEDPGPAQEASRLQKLLQECGWEYPDAALLKTMLSGASTLTPQDIETLAYGLCPAWPERAQELLSEAVRRLQGQTVPSNRHLVLVLDKDLQKLPWESIPSLRALPVTRLPSFHFLLSYSIIRETRILITSVFSSVGGLSSAKPRGGSTQYLLRPEPSQ

Target ORF information:

RefSeq Version	XM_019834990.2
Organism	Felis catus(domestic cat)
Definition	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019834990.2

ORF Insert Sequence:

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGACCAGGAT CCTGATCACC AGTGTCTTCT CCTCAGTCGG GGGCCTCAGC 
AGTGCTAAGC CAAGGGGTGG ATCCACGCAA TACCTTCTAC GTCCTGAACC CTCACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_019834990.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019690549.1 Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

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CloneID	OFb45480
Clone ID Related Accession (Same CDS sequence)	XM_019834990.2 , XM_019834990.1
Accession Version	XM_019834990.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	separin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018729.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGACCAGGAT CCTGATCACC AGTGTCTTCT CCTCAGTCGG GGGCCTCAGC 
AGTGCTAAGC CAAGGGGTGG ATCCACGCAA TACCTTCTAC GTCCTGAACC CTCACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019690549.1
CDS	166..6045
Translation	MRSFKGINFGTLLSSVKEAEELLPDLKEFLSKLPAGSNSCRSDAERRQACDAILRACNQQLTAKLACPRHLESLLELAELAYNGYLMSTPQRPPLYLERILFIFLRNIATQGIPEATLRFAKPLHACLVHCSRQAAPQDYDAVARGSFSLLWKAAETLEERRAALSTRLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDATGHSLNEADADFLDDLLSRHVIKVLVGEGGGSPGPLSPQRALCLLELTLERCRRLCWSGHHAKATRVVEKTHDYLRNTSLAPSLQLCQLGVELLQAGEGGSQALAELLIKASAVLNNSVEAPSPPLRALCDSCQFFLSGLERGIKRRYGLDAVLGLFAFLGGYCSLIRQLQDGVCGDSSKQQQSLVQMHFQGLHLYTVVVYDFAQSCQVADLADLAQLVESCKSTVVWMLEALQSLSGQELTDYLGMTASYTSNLAYSFYSHKLYSEACAISEPLCQHLGLVKPDMYPEVPPEKLHRCFRLHGESLKKLGKQAQGCEMVTLWLTALQPCGPQHMAEPVTFWVRIKMDAARAGDKELQLRTLRDSLSGWDPETLVFLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHDFAQQTNCSALDAIQEALQLLESVRPETQAKDRLLDDKAQALLWFYICTLETKMQEGIERDRRAQAPNNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKAQAPAVRCLQQTAASLQTLAALYQLAAKPLQALEVLLLVRIVSQRLEDHAKAAGSSCHASQLLLMLGCPSYAQLYLEEADSSLKLLDHNTDTYLLLSLTCNLLRSQLYCVHQKVTEGASLLLSVLRDPALQRSSKAWYLLRVQALQVVASYLSLSSDSLSASLREQLCAQGWQTPEIALVDSHKLLRSIILLLLGSDVLSIQKAAVETPFLDYGENLVQKWQVLTEVLSCSEKLVSRLGHLGCVSEAKAFCLDALKLTTKLQLPHQCALFLVLKAELELARTDIDLCESDLQQVLFLLESCTEFGGLAQHLDSVKKVHLQKGKQQTRVRRPPELPEEEPFLRGPALELVATVAKEPGPVAPSTNSSPVLKAKPQPSPGFLTHLPTCDCLLCASPVLSAVCLRWALVTAGVRLALGHQAQGLDLLQVVLKGCPAATERLTQALQTTLNHKAPPSPVPSLLDEILAQAYTQLALEGLSRPSHKSLGKVLESGLKFVAARVPHLEPWRASLLLIRALAKLAGLNCCTTQLFASSWGWQPPSVKPSPGSEPSKPRSQKLSGRGRQRAASSTQPLHNTSLKSLEGGGLPCTPKPPGQVRPAGTRVPFTVFEEVLPTKSKPEVPKAPRVQQRVQTRLKVNFSDDSDLEDPVSIEARPAEGPKRRGTASRGRGRGRGQARKNPSLKTVAVAASSSAPGLPGLGGRSRRAKKVTSGHCEELGSEIIRTIPEEEMTDNQMEMSFEILRGSDGEDSASGGKAPAPGPDTAIGECEVLRRDSSKEELPVPCPDKERDKDLGPRLRLSSAPVAIGLSTLDSICDLLTIAFRGVSHCPPSGLYAYLCRLLALCLGHRDPYATACLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEMADQVQRLSLQDRPGDVPLARIQHLFSFRASGSGQFPEPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQSKLPLSSALKEFDTIQKEQKENSSCTDKREWWTGRLELDHRMEALIISLEKHVLGCWRGLLLPSSEDPGPAQEASRLQKLLQECGWEYPDAALLKTMLSGASTLTPQDIETLAYGLCPAWPERAQELLSEAVRRLQGQTVPSNRHLVLVLDKDLQKLPWESIPSLRALPVTRLPSFHFLLSYSIIRETRILITSVFSSVGGLSSAKPRGGSTQYLLRPEPSQ

Target ORF information:

RefSeq Version	XM_019834990.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019834990.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGACCAGGAT CCTGATCACC AGTGTCTTCT CCTCAGTCGG GGGCCTCAGC 
AGTGCTAAGC CAAGGGGTGG ATCCACGCAA TACCTTCTAC GTCCTGAACC CTCACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_003988757.3 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003988806.1 Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

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CloneID	OFb09833
Clone ID Related Accession (Same CDS sequence)	XM_003988757.3 , XM_003988757.4 , XM_019834989.1
Accession Version	XM_003988757.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	separin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018729.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 29, 2016 this sequence version replaced XM_003988757.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGTCGGGGGC CTCAGCAGTG CTAAGCCAAG GGGTGGATCC ACGCAATACC 
TTCTACGTCC TGAACCCTCA CAATAATCTG TCAAGCACAG AGGAGCAATT TCGAGCCAAT 
TTTAGCAGTG AAGCTGGCTG GAAAGGGGTG GTTGGGGAGG TGCCGAGCCC AGAACAAGTG 
CAAGCAGCCT TGACAGAGCA TGACTTATAT ATCTATGCAG GGCATGGGGC AGGTGCCCGC 
TTCCTGGATG GACAGGCCGT CCTGCGGCTC GGCTGCCGGG CAGTGGCCCT GCTGTTTGGC 
TGCAGCAGCG CAGCCCTGGC TGTGCATGGA AACCTGGAGG GGGCTGGCAT CGTGCTCAAG 
TATATCATGG CTGGCTGTCC CCTGTTTCTG GGTAACCTCT GGGATGTGAC TGACCGTGAC 
ATTGACCGCT ACACGGAGGC TCTGCTGCAG GGTTGGCTTG GAGCAGGCCC AGGAGCCCCT 
CTTCTCTACT ATGTCAACCA GGCGCGCCAG GCTCCCCGAC TCAAGTATCT TATTGGGGCT 
GCACCTGTGG CATATGGCTT GCCTGTCTCC CTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003988806.1
CDS	164..6502
Translation	MRSFKGINFGTLLSSVKEAEELLPDLKEFLSKLPAGSNSCRSDAERRQACDAILRACNQQLTAKLACPRHLESLLELAELAYNGYLMSTPQRPPLYLERILFIFLRNIATQGIPEATLRFAKPLHACLVHCSRQAAPQDYDAVARGSFSLLWKAAETLEERRAALSTRLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDATGHSLNEADADFLDDLLSRHVIKVLVGEGGGSPGPLSPQRALCLLELTLERCRRLCWSGHHAKATRVVEKTHDYLRNTSLAPSLQLCQLGVELLQAGEGGSQALAELLIKASAVLNNSVEAPSPPLRALCDSCQFFLSGLERGIKRRYGLDAVLGLFAFLGGYCSLIRQLQDGVCGDSSKQQQSLVQMHFQGLHLYTVVVYDFAQSCQVADLADLAQLVESCKSTVVWMLEALQSLSGQELTDYLGMTASYTSNLAYSFYSHKLYSEACAISEPLCQHLGLVKPDMYPEVPPEKLHRCFRLHGESLKKLGKQAQGCEMVTLWLTALQPCGPQHMAEPVTFWVRIKMDAARAGDKELQLRTLRDSLSGWDPETLVFLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHDFAQQTNCSALDAIQEALQLLESVRPETQAKDRLLDDKAQALLWFYICTLETKMQEGIERDRRAQAPNNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKAQAPAVRCLQQTAASLQTLAALYQLAAKPLQALEVLLLVRIVSQRLEDHAKAAGSSCHASQLLLMLGCPSYAQLYLEEADSSLKLLDHNTDTYLLLSLTCNLLRSQLYCVHQKVTEGASLLLSVLRDPALQRSSKAWYLLRVQALQVVASYLSLSSDSLSASLREQLCAQGWQTPEIALVDSHKLLRSIILLLLGSDVLSIQKAAVETPFLDYGENLVQKWQVLTEVLSCSEKLVSRLGHLGCVSEAKAFCLDALKLTTKLQLPHQCALFLVLKAELELARTDIDLCESDLQQVLFLLESCTEFGGLAQHLDSVKKVHLQKGKQQTRVRRPPELPEEEPFLRGPALELVATVAKEPGPVAPSTNSSPVLKAKPQPSPGFLTHLPTCDCLLCASPVLSAVCLRWALVTAGVRLALGHQAQGLDLLQVVLKGCPAATERLTQALQTTLNHKAPPSPVPSLLDEILAQAYTQLALEGLSRPSHKSLGKVLESGLKFVAARVPHLEPWRASLLLIRALAKLAGLNCCTTQLFASSWGWQPPSVKPSPGSEPSKPRSQKLSGRGRQRAASSTQPLHNTSLKSLEGGGLPCTPKPPGQVRPAGTRVPFTVFEEVLPTKSKPEVPKAPRVQQRVQTRLKVNFSDDSDLEDPVSIEARPAEGPKRRGTASRGRGRGRGQARKNPSLKTVAVAASSSAPGLPGLGGRSRRAKKVTSGHCEELGSEIIRTIPEEEMTDNQMEMSFEILRGSDGEDSASGGKAPAPGPDTAIGECEVLRRDSSKEELPVPCPDKERDKDLGPRLRLSSAPVAIGLSTLDSICDLLTIAFRGVSHCPPSGLYAYLCRLLALCLGHRDPYATACLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEMADQVQRLSLQDRPGDVPLARIQHLFSFRASGSGQFPEPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQSKLPLSSALKEFDTIQKEQKENSSCTDKREWWTGRLELDHRMEALIISLEKHVLGCWRGLLLPSSEDPGPAQEASRLQKLLQECGWEYPDAALLKTMLSGASTLTPQDIETLAYGLCPAWPERAQELLSEAVRRLQGQTVPSNRHLVLVLDKDLQKLPWESIPSLRALPVTRLPSFHFLLSYSIIRESGASAVLSQGVDPRNTFYVLNPHNNLSSTEEQFRANFSSEAGWKGVVGEVPSPEQVQAALTEHDLYIYAGHGAGARFLDGQAVLRLGCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPVAYGLPVSLR

Target ORF information:

RefSeq Version	XM_003988757.3
Organism	Felis catus(Domestic cat)
Definition	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003988757.3

ORF Insert Sequence:

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGTCGGGGGC CTCAGCAGTG CTAAGCCAAG GGGTGGATCC ACGCAATACC 
TTCTACGTCC TGAACCCTCA CAATAATCTG TCAAGCACAG AGGAGCAATT TCGAGCCAAT 
TTTAGCAGTG AAGCTGGCTG GAAAGGGGTG GTTGGGGAGG TGCCGAGCCC AGAACAAGTG 
CAAGCAGCCT TGACAGAGCA TGACTTATAT ATCTATGCAG GGCATGGGGC AGGTGCCCGC 
TTCCTGGATG GACAGGCCGT CCTGCGGCTC GGCTGCCGGG CAGTGGCCCT GCTGTTTGGC 
TGCAGCAGCG CAGCCCTGGC TGTGCATGGA AACCTGGAGG GGGCTGGCAT CGTGCTCAAG 
TATATCATGG CTGGCTGTCC CCTGTTTCTG GGTAACCTCT GGGATGTGAC TGACCGTGAC 
ATTGACCGCT ACACGGAGGC TCTGCTGCAG GGTTGGCTTG GAGCAGGCCC AGGAGCCCCT 
CTTCTCTACT ATGTCAACCA GGCGCGCCAG GCTCCCCGAC TCAAGTATCT TATTGGGGCT 
GCACCTGTGG CATATGGCTT GCCTGTCTCC CTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_003988757.4 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_003988806.1 Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

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CloneID	OFb09833
Clone ID Related Accession (Same CDS sequence)	XM_003988757.3 , XM_003988757.4 , XM_019834989.1
Accession Version	XM_003988757.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018729.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 12, 2017 this sequence version replaced XM_003988757.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGTCGGGGGC CTCAGCAGTG CTAAGCCAAG GGGTGGATCC ACGCAATACC 
TTCTACGTCC TGAACCCTCA CAATAATCTG TCAAGCACAG AGGAGCAATT TCGAGCCAAT 
TTTAGCAGTG AAGCTGGCTG GAAAGGGGTG GTTGGGGAGG TGCCGAGCCC AGAACAAGTG 
CAAGCAGCCT TGACAGAGCA TGACTTATAT ATCTATGCAG GGCATGGGGC AGGTGCCCGC 
TTCCTGGATG GACAGGCCGT CCTGCGGCTC GGCTGCCGGG CAGTGGCCCT GCTGTTTGGC 
TGCAGCAGCG CAGCCCTGGC TGTGCATGGA AACCTGGAGG GGGCTGGCAT CGTGCTCAAG 
TATATCATGG CTGGCTGTCC CCTGTTTCTG GGTAACCTCT GGGATGTGAC TGACCGTGAC 
ATTGACCGCT ACACGGAGGC TCTGCTGCAG GGTTGGCTTG GAGCAGGCCC AGGAGCCCCT 
CTTCTCTACT ATGTCAACCA GGCGCGCCAG GCTCCCCGAC TCAAGTATCT TATTGGGGCT 
GCACCTGTGG CATATGGCTT GCCTGTCTCC CTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003988806.1
CDS	173..6511
Translation	MRSFKGINFGTLLSSVKEAEELLPDLKEFLSKLPAGSNSCRSDAERRQACDAILRACNQQLTAKLACPRHLESLLELAELAYNGYLMSTPQRPPLYLERILFIFLRNIATQGIPEATLRFAKPLHACLVHCSRQAAPQDYDAVARGSFSLLWKAAETLEERRAALSTRLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDATGHSLNEADADFLDDLLSRHVIKVLVGEGGGSPGPLSPQRALCLLELTLERCRRLCWSGHHAKATRVVEKTHDYLRNTSLAPSLQLCQLGVELLQAGEGGSQALAELLIKASAVLNNSVEAPSPPLRALCDSCQFFLSGLERGIKRRYGLDAVLGLFAFLGGYCSLIRQLQDGVCGDSSKQQQSLVQMHFQGLHLYTVVVYDFAQSCQVADLADLAQLVESCKSTVVWMLEALQSLSGQELTDYLGMTASYTSNLAYSFYSHKLYSEACAISEPLCQHLGLVKPDMYPEVPPEKLHRCFRLHGESLKKLGKQAQGCEMVTLWLTALQPCGPQHMAEPVTFWVRIKMDAARAGDKELQLRTLRDSLSGWDPETLVFLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHDFAQQTNCSALDAIQEALQLLESVRPETQAKDRLLDDKAQALLWFYICTLETKMQEGIERDRRAQAPNNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKAQAPAVRCLQQTAASLQTLAALYQLAAKPLQALEVLLLVRIVSQRLEDHAKAAGSSCHASQLLLMLGCPSYAQLYLEEADSSLKLLDHNTDTYLLLSLTCNLLRSQLYCVHQKVTEGASLLLSVLRDPALQRSSKAWYLLRVQALQVVASYLSLSSDSLSASLREQLCAQGWQTPEIALVDSHKLLRSIILLLLGSDVLSIQKAAVETPFLDYGENLVQKWQVLTEVLSCSEKLVSRLGHLGCVSEAKAFCLDALKLTTKLQLPHQCALFLVLKAELELARTDIDLCESDLQQVLFLLESCTEFGGLAQHLDSVKKVHLQKGKQQTRVRRPPELPEEEPFLRGPALELVATVAKEPGPVAPSTNSSPVLKAKPQPSPGFLTHLPTCDCLLCASPVLSAVCLRWALVTAGVRLALGHQAQGLDLLQVVLKGCPAATERLTQALQTTLNHKAPPSPVPSLLDEILAQAYTQLALEGLSRPSHKSLGKVLESGLKFVAARVPHLEPWRASLLLIRALAKLAGLNCCTTQLFASSWGWQPPSVKPSPGSEPSKPRSQKLSGRGRQRAASSTQPLHNTSLKSLEGGGLPCTPKPPGQVRPAGTRVPFTVFEEVLPTKSKPEVPKAPRVQQRVQTRLKVNFSDDSDLEDPVSIEARPAEGPKRRGTASRGRGRGRGQARKNPSLKTVAVAASSSAPGLPGLGGRSRRAKKVTSGHCEELGSEIIRTIPEEEMTDNQMEMSFEILRGSDGEDSASGGKAPAPGPDTAIGECEVLRRDSSKEELPVPCPDKERDKDLGPRLRLSSAPVAIGLSTLDSICDLLTIAFRGVSHCPPSGLYAYLCRLLALCLGHRDPYATACLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEMADQVQRLSLQDRPGDVPLARIQHLFSFRASGSGQFPEPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQSKLPLSSALKEFDTIQKEQKENSSCTDKREWWTGRLELDHRMEALIISLEKHVLGCWRGLLLPSSEDPGPAQEASRLQKLLQECGWEYPDAALLKTMLSGASTLTPQDIETLAYGLCPAWPERAQELLSEAVRRLQGQTVPSNRHLVLVLDKDLQKLPWESIPSLRALPVTRLPSFHFLLSYSIIRESGASAVLSQGVDPRNTFYVLNPHNNLSSTEEQFRANFSSEAGWKGVVGEVPSPEQVQAALTEHDLYIYAGHGAGARFLDGQAVLRLGCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPVAYGLPVSLR

Target ORF information:

RefSeq Version	XM_003988757.4
Organism	Felis catus(domestic cat)
Definition	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003988757.4

ORF Insert Sequence:

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGTCGGGGGC CTCAGCAGTG CTAAGCCAAG GGGTGGATCC ACGCAATACC 
TTCTACGTCC TGAACCCTCA CAATAATCTG TCAAGCACAG AGGAGCAATT TCGAGCCAAT 
TTTAGCAGTG AAGCTGGCTG GAAAGGGGTG GTTGGGGAGG TGCCGAGCCC AGAACAAGTG 
CAAGCAGCCT TGACAGAGCA TGACTTATAT ATCTATGCAG GGCATGGGGC AGGTGCCCGC 
TTCCTGGATG GACAGGCCGT CCTGCGGCTC GGCTGCCGGG CAGTGGCCCT GCTGTTTGGC 
TGCAGCAGCG CAGCCCTGGC TGTGCATGGA AACCTGGAGG GGGCTGGCAT CGTGCTCAAG 
TATATCATGG CTGGCTGTCC CCTGTTTCTG GGTAACCTCT GGGATGTGAC TGACCGTGAC 
ATTGACCGCT ACACGGAGGC TCTGCTGCAG GGTTGGCTTG GAGCAGGCCC AGGAGCCCCT 
CTTCTCTACT ATGTCAACCA GGCGCGCCAG GCTCCCCGAC TCAAGTATCT TATTGGGGCT 
GCACCTGTGG CATATGGCTT GCCTGTCTCC CTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_019834989.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019690548.1 Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OFb09833
Clone ID Related Accession (Same CDS sequence)	XM_003988757.3 , XM_003988757.4 , XM_019834989.1
Accession Version	XM_019834989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	separin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018729.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGTCGGGGGC CTCAGCAGTG CTAAGCCAAG GGGTGGATCC ACGCAATACC 
TTCTACGTCC TGAACCCTCA CAATAATCTG TCAAGCACAG AGGAGCAATT TCGAGCCAAT 
TTTAGCAGTG AAGCTGGCTG GAAAGGGGTG GTTGGGGAGG TGCCGAGCCC AGAACAAGTG 
CAAGCAGCCT TGACAGAGCA TGACTTATAT ATCTATGCAG GGCATGGGGC AGGTGCCCGC 
TTCCTGGATG GACAGGCCGT CCTGCGGCTC GGCTGCCGGG CAGTGGCCCT GCTGTTTGGC 
TGCAGCAGCG CAGCCCTGGC TGTGCATGGA AACCTGGAGG GGGCTGGCAT CGTGCTCAAG 
TATATCATGG CTGGCTGTCC CCTGTTTCTG GGTAACCTCT GGGATGTGAC TGACCGTGAC 
ATTGACCGCT ACACGGAGGC TCTGCTGCAG GGTTGGCTTG GAGCAGGCCC AGGAGCCCCT 
CTTCTCTACT ATGTCAACCA GGCGCGCCAG GCTCCCCGAC TCAAGTATCT TATTGGGGCT 
GCACCTGTGG CATATGGCTT GCCTGTCTCC CTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019690548.1
CDS	110..6448
Translation	MRSFKGINFGTLLSSVKEAEELLPDLKEFLSKLPAGSNSCRSDAERRQACDAILRACNQQLTAKLACPRHLESLLELAELAYNGYLMSTPQRPPLYLERILFIFLRNIATQGIPEATLRFAKPLHACLVHCSRQAAPQDYDAVARGSFSLLWKAAETLEERRAALSTRLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDATGHSLNEADADFLDDLLSRHVIKVLVGEGGGSPGPLSPQRALCLLELTLERCRRLCWSGHHAKATRVVEKTHDYLRNTSLAPSLQLCQLGVELLQAGEGGSQALAELLIKASAVLNNSVEAPSPPLRALCDSCQFFLSGLERGIKRRYGLDAVLGLFAFLGGYCSLIRQLQDGVCGDSSKQQQSLVQMHFQGLHLYTVVVYDFAQSCQVADLADLAQLVESCKSTVVWMLEALQSLSGQELTDYLGMTASYTSNLAYSFYSHKLYSEACAISEPLCQHLGLVKPDMYPEVPPEKLHRCFRLHGESLKKLGKQAQGCEMVTLWLTALQPCGPQHMAEPVTFWVRIKMDAARAGDKELQLRTLRDSLSGWDPETLVFLLREELQAYKAVRADTGQERFNVICDLLELSPEETPAGAWARATHLVELAQVLCYHDFAQQTNCSALDAIQEALQLLESVRPETQAKDRLLDDKAQALLWFYICTLETKMQEGIERDRRAQAPNNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKEVLTKAQAPAVRCLQQTAASLQTLAALYQLAAKPLQALEVLLLVRIVSQRLEDHAKAAGSSCHASQLLLMLGCPSYAQLYLEEADSSLKLLDHNTDTYLLLSLTCNLLRSQLYCVHQKVTEGASLLLSVLRDPALQRSSKAWYLLRVQALQVVASYLSLSSDSLSASLREQLCAQGWQTPEIALVDSHKLLRSIILLLLGSDVLSIQKAAVETPFLDYGENLVQKWQVLTEVLSCSEKLVSRLGHLGCVSEAKAFCLDALKLTTKLQLPHQCALFLVLKAELELARTDIDLCESDLQQVLFLLESCTEFGGLAQHLDSVKKVHLQKGKQQTRVRRPPELPEEEPFLRGPALELVATVAKEPGPVAPSTNSSPVLKAKPQPSPGFLTHLPTCDCLLCASPVLSAVCLRWALVTAGVRLALGHQAQGLDLLQVVLKGCPAATERLTQALQTTLNHKAPPSPVPSLLDEILAQAYTQLALEGLSRPSHKSLGKVLESGLKFVAARVPHLEPWRASLLLIRALAKLAGLNCCTTQLFASSWGWQPPSVKPSPGSEPSKPRSQKLSGRGRQRAASSTQPLHNTSLKSLEGGGLPCTPKPPGQVRPAGTRVPFTVFEEVLPTKSKPEVPKAPRVQQRVQTRLKVNFSDDSDLEDPVSIEARPAEGPKRRGTASRGRGRGRGQARKNPSLKTVAVAASSSAPGLPGLGGRSRRAKKVTSGHCEELGSEIIRTIPEEEMTDNQMEMSFEILRGSDGEDSASGGKAPAPGPDTAIGECEVLRRDSSKEELPVPCPDKERDKDLGPRLRLSSAPVAIGLSTLDSICDLLTIAFRGVSHCPPSGLYAYLCRLLALCLGHRDPYATACLVTESVSITCRHQLLTHLHRQLSKAQKHRGSLEMADQVQRLSLQDRPGDVPLARIQHLFSFRASGSGQFPEPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDNPPVTVQIPTAQSKLPLSSALKEFDTIQKEQKENSSCTDKREWWTGRLELDHRMEALIISLEKHVLGCWRGLLLPSSEDPGPAQEASRLQKLLQECGWEYPDAALLKTMLSGASTLTPQDIETLAYGLCPAWPERAQELLSEAVRRLQGQTVPSNRHLVLVLDKDLQKLPWESIPSLRALPVTRLPSFHFLLSYSIIRESGASAVLSQGVDPRNTFYVLNPHNNLSSTEEQFRANFSSEAGWKGVVGEVPSPEQVQAALTEHDLYIYAGHGAGARFLDGQAVLRLGCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPVAYGLPVSLR

Target ORF information:

RefSeq Version	XM_019834989.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019834989.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAGGAAT CAACTTTGGG ACCTTGCTAA GCAGCGTGAA GGAAGCTGAA 
GAGTTGCTGC CTGACTTGAA GGAGTTCCTG TCCAAGCTTC CAGCTGGTTC AAACAGCTGC 
CGATCTGATG CCGAGAGGAG ACAAGCTTGT GATGCCATCT TGAGGGCTTG CAACCAGCAG 
CTGACTGCCA AGCTGGCTTG CCCTAGGCAC CTGGAGAGCC TGCTGGAACT GGCAGAGCTG 
GCTTACAATG GCTACTTGAT GTCTACCCCC CAGCGCCCAC CCCTGTACCT GGAACGAATT 
CTCTTCATCT TTTTGCGGAA CATTGCCACA CAGGGAATCC CAGAGGCCAC GCTCCGATTC 
GCAAAACCCC TTCATGCCTG TTTGGTGCAT TGCTCTCGCC AAGCTGCTCC CCAGGACTAT 
GATGCTGTGG CGCGAGGCAG CTTTTCTCTG CTTTGGAAGG CAGCAGAAAC CCTGGAGGAG 
CGGCGAGCTG CATTATCAAC TCGGCTGAAG GCCTTGAGCT TTCTTGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCCCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CACTGGGCAC AGTCTGAATG AAGCCGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA TGTGATCAAA GTTTTGGTGG GTGAGGGAGG GGGTTCTCCT 
GGTCCTCTTT CTCCCCAGAG GGCCCTCTGT CTCTTGGAAC TCACCCTGGA ACGTTGCCGT 
CGCCTCTGCT GGAGTGGCCA CCATGCCAAG GCCACGAGAG TAGTGGAGAA GACCCATGAT 
TACCTAAGGA ACACCAGTCT GGCTCCCAGC CTCCAGTTAT GCCAACTTGG GGTTGAACTG 
CTACAGGCTG GGGAGGGAGG ATCCCAGGCA CTGGCCGAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAACA ACAGTGTGGA GGCACCATCA CCCCCATTGC GGGCATTATG TGACAGCTGC 
CAGTTCTTCC TCTCAGGACT GGAGCGAGGC ATCAAGAGAC GCTATGGACT GGATGCTGTT 
CTGGGCCTCT TTGCTTTTCT TGGCGGGTAC TGCTCTCTCA TCCGGCAGCT GCAGGATGGT 
GTCTGTGGGG ATTCCTCCAA GCAGCAGCAG TCTCTGGTTC AGATGCACTT TCAGGGACTT 
CACCTCTACA CTGTGGTGGT TTATGACTTT GCACAAAGCT GTCAGGTAGC TGATTTGGCT 
GACCTGGCCC AGCTGGTGGA AAGTTGCAAA TCTACTGTGG TCTGGATGCT GGAGGCCTTA 
CAGAGCCTGT CAGGCCAAGA GCTGACTGAC TACCTGGGCA TGACTGCCTC TTACACCAGT 
AACTTGGCCT ACAGCTTCTA TAGTCACAAG CTGTATTCCG AGGCCTGTGC CATCTCTGAG 
CCCCTCTGTC AGCACCTGGG GTTGGTGAAG CCGGACATGT ATCCTGAGGT GCCTCCTGAG 
AAGTTGCACA GGTGCTTCCG GCTGCATGGA GAGAGTTTGA AGAAACTGGG TAAACAGGCC 
CAGGGCTGCG AGATGGTGAC TTTGTGGCTG ACAGCCCTGC AGCCCTGTGG CCCCCAACAC 
ATGGCTGAGC CTGTCACCTT CTGGGTTCGG ATCAAGATGG ATGCAGCCAG AGCAGGAGAC 
AAGGAGCTAC AGCTGAGGAC TCTGCGAGAC AGCCTGAGTG GCTGGGACCC AGAGACCCTG 
GTCTTCCTGC TGAGGGAGGA GCTGCAGGCC TATAAGGCTG TGCGAGCGGA CACAGGGCAG 
GAACGGTTCA ACGTCATCTG TGACCTGCTG GAGCTGAGCC CTGAGGAGAC ACCGGCTGGG 
GCCTGGGCTC GAGCCACCCA CCTGGTGGAA CTGGCTCAGG TGCTCTGCTA CCATGACTTT 
GCCCAGCAGA CCAACTGCTC TGCCCTGGAT GCTATCCAGG AAGCCCTACA GCTTCTGGAG 
TCTGTGAGGC CTGAGACCCA GGCCAAGGAT CGGCTTCTGG ATGATAAAGC ACAGGCCCTC 
CTATGGTTCT ACATTTGTAC TCTGGAGACC AAAATGCAGG AAGGTATTGA GCGGGATCGG 
AGAGCCCAGG CCCCTAATAA CCTGGAGGAA TTTGAAGTCA ATGACCTGAA CTATGAAGAT 
AAACTCCAAG AAGATCGTTT CCTATACAGT AACATTGCCT TCAACCTGGC TGCAGATGCT 
GCTCAGTCCA AATGCCTGGA CCAAGCACTG GCCCTTTGGA AGGAGGTGCT TACAAAGGCA 
CAAGCCCCCG CTGTACGGTG TCTCCAGCAG ACAGCAGCCT CACTGCAGAC CCTAGCAGCC 
CTCTATCAGC TGGCGGCAAA GCCCCTGCAG GCTCTGGAGG TCCTCCTGCT CGTACGGATC 
GTCTCCCAGA GACTGGAGGA CCACGCGAAG GCAGCCGGCT CCTCCTGCCA CGCCAGCCAG 
CTGCTCCTGA TGCTCGGCTG TCCCAGCTAT GCCCAGCTAT ACCTGGAAGA GGCAGACTCA 
AGCCTGAAGC TTCTGGATCA CAACACTGAC ACGTACCTGC TCCTTTCGCT GACCTGTAAC 
CTACTTCGAA GTCAACTCTA CTGTGTTCAC CAGAAGGTGA CTGAGGGTGC CTCTCTGTTG 
CTGTCTGTGC TTCGGGACCC TGCCCTCCAG AGATCATCCA AGGCCTGGTA CTTGCTGCGT 
GTCCAGGCCT TGCAGGTGGT GGCATCGTAC CTCAGCCTCT CATCAGACAG CCTCTCAGCC 
TCCCTGCGGG AGCAGCTCTG TGCCCAAGGC TGGCAGACGC CTGAGATTGC ACTCGTCGAC 
TCCCATAAGC TCCTCCGAAG CATTATCCTC CTGCTCCTGG GCAGTGACGT GCTCTCGATT 
CAGAAGGCGG CTGTGGAGAC CCCATTTCTG GACTATGGTG AAAATCTGGT ACAAAAATGG 
CAGGTTCTGA CAGAGGTGCT GAGCTGCTCA GAGAAGCTGG TCTCCCGCCT GGGCCATCTG 
GGTTGTGTGA GCGAAGCCAA GGCCTTTTGC TTGGATGCCC TGAAACTTAC GACGAAACTG 
CAGCTACCAC ACCAGTGTGC CCTGTTCCTG GTGCTGAAGG CGGAGCTGGA GCTGGCACGC 
ACTGACATTG ACCTCTGTGA GTCGGACCTG CAGCAGGTTC TGTTCTTACT GGAGTCTTGC 
ACAGAGTTTG GCGGGCTAGC CCAGCACCTG GACTCAGTGA AGAAAGTCCA CCTGCAGAAG 
GGCAAGCAGC AGACCCGGGT CCGTCGTCCT CCAGAGCTCC CTGAAGAGGA GCCCTTCCTA 
AGAGGCCCTG CCCTGGAGCT GGTAGCCACT GTGGCCAAGG AGCCAGGTCC TGTGGCACCT 
TCTACTAACT CCTCCCCTGT CCTGAAAGCG AAGCCCCAGC CCAGCCCCGG CTTCCTGACC 
CACCTGCCCA CGTGTGACTG CTTGCTCTGT GCCAGCCCCG TGCTCTCGGC AGTCTGTCTG 
CGCTGGGCAT TGGTCACAGC GGGGGTGAGG CTGGCTTTGG GCCATCAGGC CCAGGGTCTG 
GATCTGCTGC AGGTCGTGCT AAAGGGTTGC CCTGCAGCCA CTGAGCGCCT CACCCAAGCT 
CTACAAACTA CCCTGAATCA CAAAGCACCT CCCTCCCCTG TCCCGAGCCT CTTGGATGAG 
ATCTTGGCTC AGGCCTACAC ACAGCTGGCA CTGGAGGGGC TGAGCCGGCC ATCACACAAG 
AGTCTGGGGA AGGTCCTGGA GTCGGGGCTG AAGTTTGTGG CAGCACGAGT ACCCCACCTG 
GAGCCCTGGC GAGCCAGCCT GCTCTTGATT CGGGCCCTTG CAAAGCTGGC CGGCCTCAAC 
TGCTGCACTA CCCAACTTTT TGCAAGCTCC TGGGGCTGGC AGCCACCATC AGTAAAACCC 
TCCCCAGGCT CAGAGCCCTC TAAGCCGCGA AGCCAAAAAC TTTCTGGAAG GGGGCGTCAG 
CGGGCAGCCT CATCTACCCA GCCCCTCCAT AATACCTCTC TGAAGAGTCT GGAAGGTGGA 
GGACTGCCCT GTACACCGAA GCCCCCAGGC CAGGTCAGGC CAGCAGGCAC TCGAGTCCCT 
TTTACTGTGT TTGAGGAAGT CCTCCCTACA AAGAGCAAGC CTGAGGTTCC CAAGGCCCCC 
AGAGTACAAC AGAGGGTCCA GACGCGCCTC AAGGTGAACT TCAGTGATGA CAGTGATTTG 
GAAGACCCTG TCTCAATTGA AGCACGGCCT GCAGAGGGGC CCAAGAGACG GGGCACTGCT 
TCCCGGGGCC GGGGCCGGGG CCGGGGCCAG GCCAGGAAGA ACCCGAGCCT GAAGACAGTT 
GCAGTGGCAG CCTCAAGTAG TGCCCCTGGG CTCCCAGGCC TGGGTGGTAG GAGCCGGAGG 
GCCAAGAAGG TGACATCAGG ACACTGTGAG GAGCTGGGCT CTGAGATCAT AAGGACCATC 
CCTGAGGAGG AAATGACTGA CAACCAGATG GAAATGAGCT TTGAGATCCT CAGGGGCTCT 
GACGGGGAAG ACTCAGCCTC AGGTGGGAAG GCACCAGCTC CAGGACCTGA TACAGCCATA 
GGAGAATGTG AAGTGTTGAG ACGTGATTCC AGTAAAGAGG AGCTGCCAGT CCCATGCCCA 
GACAAGGAGA GAGACAAGGA TCTTGGTCCT CGGCTCCGAC TCTCCTCTGC CCCTGTAGCC 
ATTGGTCTCT CTACCCTGGA TTCCATCTGT GACTTATTGA CCATTGCTTT CCGTGGGGTT 
AGTCATTGCC CTCCTAGTGG ACTTTATGCT TACCTCTGCC GCCTCCTGGC TTTGTGCTTG 
GGCCACCGGG ATCCCTATGC CACAGCCTGC CTTGTCACTG AGTCTGTGTC CATCACCTGT 
CGCCACCAGC TGCTCACCCA CCTCCACAGG CAGCTCAGCA AGGCCCAGAA GCACCGGGGA 
TCACTCGAAA TGGCAGACCA GGTGCAGAGG CTGAGCCTGC AGGACAGGCC TGGAGATGTC 
CCCCTGGCCC GCATCCAGCA CCTCTTTTCC TTCAGGGCTT CAGGATCTGG TCAATTCCCT 
GAGCCTGAGA AGGAGAGTTT CCAGGAGCGC CTGGCTCTGA TCCCCAGCGG GGTAACTGTG 
TGTGTGTTAG CTCTGGCCAC ACTTCAGCCT GGAACCGTGG GCAACACCCT CTTGCTGACC 
CGACTGGAAA AAGACAATCC CCCAGTCACT GTGCAGATCC CCACCGCCCA GAGCAAGCTT 
CCTCTGAGTT CGGCCCTGAA AGAGTTTGAC ACCATCCAGA AGGAACAGAA AGAGAACAGC 
AGCTGTACTG ACAAGCGAGA ATGGTGGACA GGACGGCTAG AACTGGACCA TAGGATGGAG 
GCACTCATCA TTTCCCTAGA GAAACATGTA TTGGGCTGCT GGAGGGGACT GTTGCTGCCA 
TCCAGTGAGG ACCCTGGCCC TGCCCAGGAG GCCTCCCGCC TACAAAAGTT GCTGCAGGAA 
TGTGGCTGGG AATATCCTGA TGCAGCTCTG CTGAAAACCA TGCTCAGTGG TGCCAGTACC 
CTCACCCCTC AGGACATTGA GACCTTGGCC TATGGGCTGT GCCCAGCCTG GCCAGAACGA 
GCCCAAGAGC TTCTAAGTGA GGCAGTACGA CGTCTTCAGG GTCAGACAGT ACCAAGCAAT 
AGGCATCTTG TCTTAGTGCT AGACAAGGAC CTGCAAAAGC TGCCATGGGA GAGCATCCCC 
AGTCTCCGGG CACTGCCTGT CACTCGGCTG CCCTCCTTCC ACTTCTTGCT CAGCTATTCC 
ATCATCAGAG AGTCGGGGGC CTCAGCAGTG CTAAGCCAAG GGGTGGATCC ACGCAATACC 
TTCTACGTCC TGAACCCTCA CAATAATCTG TCAAGCACAG AGGAGCAATT TCGAGCCAAT 
TTTAGCAGTG AAGCTGGCTG GAAAGGGGTG GTTGGGGAGG TGCCGAGCCC AGAACAAGTG 
CAAGCAGCCT TGACAGAGCA TGACTTATAT ATCTATGCAG GGCATGGGGC AGGTGCCCGC 
TTCCTGGATG GACAGGCCGT CCTGCGGCTC GGCTGCCGGG CAGTGGCCCT GCTGTTTGGC 
TGCAGCAGCG CAGCCCTGGC TGTGCATGGA AACCTGGAGG GGGCTGGCAT CGTGCTCAAG 
TATATCATGG CTGGCTGTCC CCTGTTTCTG GGTAACCTCT GGGATGTGAC TGACCGTGAC 
ATTGACCGCT ACACGGAGGC TCTGCTGCAG GGTTGGCTTG GAGCAGGCCC AGGAGCCCCT 
CTTCTCTACT ATGTCAACCA GGCGCGCCAG GCTCCCCGAC TCAAGTATCT TATTGGGGCT 
GCACCTGTGG CATATGGCTT GCCTGTCTCC CTGCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ESPL1 cDNA ORF clone, Felis catus(Domestic cat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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