AFDN gene cDNA ORF clone, Felis catus(Domestic cat)

mRNA	Protein	Name
XM_023254581.1	XP_023110349.1	afadin isoform X4
XM_023254588.1	XP_023110356.1	afadin isoform X11
XM_023254592.1	XP_023110360.1	afadin isoform X15
XM_019831490.1	XP_019687049.1	afadin isoform X3
XM_019831492.1	XP_019687051.1	afadin isoform X5
XM_019831494.1	XP_019687053.1	afadin isoform X7
XM_019831500.1	XP_019687059.1	afadin isoform X13
XM_019831502.1	XP_019687061.1	afadin isoform X15
XM_019831505.1	XP_019687064.1	afadin isoform X18
XM_023254578.1	XP_023110346.1	afadin isoform X1
XM_023254583.1	XP_023110351.1	afadin isoform X6
XM_023254586.1	XP_023110354.1	afadin isoform X9
XM_019831491.1	XP_019687050.1	afadin isoform X4
XM_019831499.1	XP_019687058.1	afadin isoform X12
XM_019831501.1	XP_019687060.1	afadin isoform X14
XM_019831503.1	XP_019687062.1	afadin isoform X16
XM_019831504.1	XP_019687063.1	afadin isoform X17
XM_023254579.1	XP_023110347.1	afadin isoform X2
XM_023254582.1	XP_023110350.1	afadin isoform X5
XM_023254584.1	XP_023110352.1	afadin isoform X7
XM_023254585.1	XP_023110353.1	afadin isoform X8
XM_023254589.1	XP_023110357.1	afadin isoform X12
XM_023254591.1	XP_023110359.1	afadin isoform X14
XM_019831493.1	XP_019687052.1	afadin isoform X6
XM_019831495.1	XP_019687054.1	afadin isoform X8
XM_019831496.1	XP_019687055.1	afadin isoform X9
XM_019831497.1	XP_019687056.1	afadin isoform X10
XM_019831498.1	XP_019687057.1	afadin isoform X11
XM_023254590.1	XP_023110358.1	afadin isoform X13
XM_023254580.1	XP_023110348.1	afadin isoform X3
XM_023254587.1	XP_023110355.1	afadin isoform X10
XM_019831488.1	XP_019687047.1	afadin isoform X1
XM_019831489.1	XP_019687048.1	afadin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFb43224	XM_019831488.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43225	XM_019831489.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43226	XM_019831490.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43227	XM_019831491.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43228	XM_019831492.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43229	XM_019831493.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43230	XM_019831494.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43231	XM_019831495.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43232	XM_019831496.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43233	XM_019831497.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43234	XM_019831498.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43235	XM_019831499.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43236	XM_019831500.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43237	XM_019831501.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43238	XM_019831502.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43239	XM_019831503.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb43240	XM_019831504.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb43241	XM_019831505.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OFb76137	XM_023254578.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76138	XM_023254579.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76139	XM_023254580.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76140	XM_023254581.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76141	XM_023254582.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76142	XM_023254583.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76143	XM_023254584.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76144	XM_023254585.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76145	XM_023254586.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76146	XM_023254587.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76147	XM_023254588.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76148	XM_023254589.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76149	XM_023254590.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76150	XM_023254591.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OFb76151	XM_023254592.1 Latest version!	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

AFDN ( XM_019831488.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687047.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OFb43224
Clone ID Related Accession (Same CDS sequence)	XM_019831488.1
Accession Version	XM_019831488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGAAACA GAAGAATATC GCAGGGGCAC TTGCTTTCTG GATGGCCAAT 
GCATCTGAGC TTCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTTTAGCACA CTTGGTTCAA ATGGCGTTTA AATATCTGGT TCATTGTCTT 
CAGTCAGAAC TGAATAACTA CATGCCAGCT TTTCTGGATG ACCCTGAAGA GAACAGTCTA 
CAAAGACCAA AAATAGATGA CGTCTTGCAC ACGCTCACGG GAGCGATGGC GCTGCTGAGG 
CGCTGCAGGG TCAATGCTGC TCTGACCATC CAGCTTTTCT CTCAGCTCTT CCACTTCATC 
AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCCGAGT CGGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGG CACGTGGAAG CCTGGGCAGA GAAGCAGGGA 
TTAGAGCTGG CAGCCGACTG TCACCTGAGC CGGATAGTGC AGGCGACCAC TTTGCTCACC 
ATGGACAAAT ACGCTCCTGA GGACACCCCA AATATCAACA GCACCTGCTT TAAGTTAAAT 
TCGCTGCAGC TGCAGGCCTT GCTACAGAAT TACCACTGTG CGCCCGACGA GCCCTTTGTC 
CCCACGGACC TGATAGAGAA TGTCGTGGCC GTGGCTGAAA ACACGGCCGA CGAGCTGGCA 
CGCAGTGATG GGCGGGAGGT GCAGCTGGAA GAGGACCCTG ATCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGCTATTC TTGTGACGTC GTCAGGAACG TTCCAAACGG TTTACAAGAA 
TTTCTGGACC CTCTGTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCATGC ACGTTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCATCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GCGGAAAGAA CCTGAAATAA TCACTGTGAC TCTCAAAAAG 
CAAAACGGAA TGGGCCTCAG CATTGTCGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA 
ATCTATGTCA AGTCTGTCGT AAAAGGAGGT GCTGCAGATG TGGATGGACG CCTAGCCGCA 
GGTGACCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCA 
GCAGAACTCA TGACAAGGAC CAGTTCCGTG GTGACACTGG AAGTAGCCAA GCAAGGAGCG 
ATCTACCACG GCCTGGCCAC CCTGCTCAAC CAGCCGTCCC CGATGATGCA GAGAGTTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTCGAGCT CTATAACAAT 
TCAGCTCAAA ACGGGTCACC CGAGAGCCCT CAGCTGCCTT GGGCAGAATA CAGCGAACCG 
AAGAAGCTCC CTGGCGATGA CAGGCTGATG AAGAACCGGG CTGACCACCG CTCCAGCCCC 
AACGTGGCAA ATCAGCCCCC CAGTCCCGGA GGGAAAAGCG CGTACGCCCC TGGAGCAGCC 
GCTAAGATCA CGTCCGTCTC CACCGGAAAC CTCTGCACTG AGGAGCAGAC CCCTCCGCCT 
CGACCTGAGG CCTACCCCAT CCCCACCCAG ACATACACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGCTCCC CCTCAGAACC AGTGGCCCAA TTACGAGGAG 
AAGCCGCACG TGCACACAGA TGGTCATCAG TCCGGCCCCG CAATGCAGCG CGTCACCCGC 
TCCCAGGAGG AACTTCGGGA GGATAAAGCT TACCAACTCG AGCGGCATCG GCTGGAGGCG 
GTGCTGGATC GCAAGTCCGA CAGCGACGTG TGGATCCAGC CGAGCTCCTC GGTGGGCTCC 
AGCACGTCCA GCCAGGAGCA CCTGGACCCC TGCTCCAAGT CGGGCACCCC CGCCTCCACG 
CTGACCAAGA GCGGCCCCGG GCGCTGGAAG ACGCCAGCCT CCGCGCAGCC CCTGCCGGCG 
GCCCCCCCGC CCGCGCAGAC AGACCTGCCC CCACCACCCC CGCCGCCCCC CGCGCACTAC 
GCCGGAGACT TCGACGGGAT CCCCGTGGAC CTGCCCCTCC CGCCCCCTCC CAGCGGCCAG 
GCGGCGGCGG AGCGGAGGAA ACGGGAGGAG CAGCAGCGCT GGTACGAGAA GGAGAAGGCC 
CGCCTGGAGG AGGAGCGGGA CAGGAAGCGC CGGGAGCAGG AGCGGAAGCT GGGCCAGATG 
CGCTCCCAGG CCCTGAACCC GGCCGCGTTC TCCCCAGTGA CCGTCGCGCA CACGAAGCCG 
GAGAAACCTT CCACGCTCCA GAGGCCCCAG GACACGGTCA TCCGGGAGCT GCAGCCCCAG 
CAGCAGCCAC GCACCATCGA GCGCCGAGAC TTGCAGTACA TCACGGTCAG CAAAGAGGAG 
CTGTCCTCGG GGGACAGCCT GTCCCCCGAC CCCTGGAAGC GGGACGCCAG GGAGAAGCTG 
GAGAAGCAGC AGCAAATGCA CATCGTGGAC ATGCTGAGCA GGGAGATCCG GGAGCTCCAG 
AGCAAGCCCG AGCGCAGCGC CGAGGACAGC GACCGCCTGC GCAAGCTCAT GCTGGAGTGG 
CAGTTCCAGA AGAGACTGCA GGAGTCCAAG CAGAAGGACG AGGACGACGA GGAGGAGGAG 
GACGACGACG TGGACACCAT GCTGATCATG CAGCGCCTCG AGGCTGAGCG GAGAGCAAGG 
CAGACTGGTC TGCCAGCAAT CTCTGTTCTA GATTTGTTAC AGGACGAGGA GCGGCGGCGG 
CAGCGGCAGC TGGACGACAT GCGCAGGCGG GAAGCGGAGG ACCGCGCCAG GCAGGAGGAG 
GAGAGGACGA AGCGAGACGC CGAAGACAAG AGGCGACAGG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGCTGCT GGAGCCGGAG 
GAGCCGGGCC TGGGCAGACC GCCGCTGCCG CGGGGCTACG AGCTCCCGGC CCCGTCCTCC 
CCGGCGGCTG CTCCTCCTCC CCCACCTCAG CGGACCGCCT CCTCCCTGCA GGCCCCGGCC 
CTGTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG 
GAGGACTGCA GTCTGGCAGG TCAGGACAAG CTCTCCCACA CCGGACCAAA CTCTTCCGTG 
GGATCTTCCG GGGCAGCGAT CGGTGCCCGT GACGGCTCCC GGGACCCGAG AGAGAGGCTG 
GCGAAAAGCC ATGATGCAGA TTTACCTGGA AATTCCGGAG CCCCTGAGAA CTTGACATTC 
AAAGAGCGCC AGCGGCTGTT CTCACAAGGT CAAGATGTGT CCAATAAAGT GAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687047.1
CDS	22..5403
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831488.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831488.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGAAACA GAAGAATATC GCAGGGGCAC TTGCTTTCTG GATGGCCAAT 
GCATCTGAGC TTCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTTTAGCACA CTTGGTTCAA ATGGCGTTTA AATATCTGGT TCATTGTCTT 
CAGTCAGAAC TGAATAACTA CATGCCAGCT TTTCTGGATG ACCCTGAAGA GAACAGTCTA 
CAAAGACCAA AAATAGATGA CGTCTTGCAC ACGCTCACGG GAGCGATGGC GCTGCTGAGG 
CGCTGCAGGG TCAATGCTGC TCTGACCATC CAGCTTTTCT CTCAGCTCTT CCACTTCATC 
AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCCGAGT CGGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGG CACGTGGAAG CCTGGGCAGA GAAGCAGGGA 
TTAGAGCTGG CAGCCGACTG TCACCTGAGC CGGATAGTGC AGGCGACCAC TTTGCTCACC 
ATGGACAAAT ACGCTCCTGA GGACACCCCA AATATCAACA GCACCTGCTT TAAGTTAAAT 
TCGCTGCAGC TGCAGGCCTT GCTACAGAAT TACCACTGTG CGCCCGACGA GCCCTTTGTC 
CCCACGGACC TGATAGAGAA TGTCGTGGCC GTGGCTGAAA ACACGGCCGA CGAGCTGGCA 
CGCAGTGATG GGCGGGAGGT GCAGCTGGAA GAGGACCCTG ATCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGCTATTC TTGTGACGTC GTCAGGAACG TTCCAAACGG TTTACAAGAA 
TTTCTGGACC CTCTGTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCATGC ACGTTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCATCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GCGGAAAGAA CCTGAAATAA TCACTGTGAC TCTCAAAAAG 
CAAAACGGAA TGGGCCTCAG CATTGTCGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA 
ATCTATGTCA AGTCTGTCGT AAAAGGAGGT GCTGCAGATG TGGATGGACG CCTAGCCGCA 
GGTGACCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCA 
GCAGAACTCA TGACAAGGAC CAGTTCCGTG GTGACACTGG AAGTAGCCAA GCAAGGAGCG 
ATCTACCACG GCCTGGCCAC CCTGCTCAAC CAGCCGTCCC CGATGATGCA GAGAGTTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTCGAGCT CTATAACAAT 
TCAGCTCAAA ACGGGTCACC CGAGAGCCCT CAGCTGCCTT GGGCAGAATA CAGCGAACCG 
AAGAAGCTCC CTGGCGATGA CAGGCTGATG AAGAACCGGG CTGACCACCG CTCCAGCCCC 
AACGTGGCAA ATCAGCCCCC CAGTCCCGGA GGGAAAAGCG CGTACGCCCC TGGAGCAGCC 
GCTAAGATCA CGTCCGTCTC CACCGGAAAC CTCTGCACTG AGGAGCAGAC CCCTCCGCCT 
CGACCTGAGG CCTACCCCAT CCCCACCCAG ACATACACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGCTCCC CCTCAGAACC AGTGGCCCAA TTACGAGGAG 
AAGCCGCACG TGCACACAGA TGGTCATCAG TCCGGCCCCG CAATGCAGCG CGTCACCCGC 
TCCCAGGAGG AACTTCGGGA GGATAAAGCT TACCAACTCG AGCGGCATCG GCTGGAGGCG 
GTGCTGGATC GCAAGTCCGA CAGCGACGTG TGGATCCAGC CGAGCTCCTC GGTGGGCTCC 
AGCACGTCCA GCCAGGAGCA CCTGGACCCC TGCTCCAAGT CGGGCACCCC CGCCTCCACG 
CTGACCAAGA GCGGCCCCGG GCGCTGGAAG ACGCCAGCCT CCGCGCAGCC CCTGCCGGCG 
GCCCCCCCGC CCGCGCAGAC AGACCTGCCC CCACCACCCC CGCCGCCCCC CGCGCACTAC 
GCCGGAGACT TCGACGGGAT CCCCGTGGAC CTGCCCCTCC CGCCCCCTCC CAGCGGCCAG 
GCGGCGGCGG AGCGGAGGAA ACGGGAGGAG CAGCAGCGCT GGTACGAGAA GGAGAAGGCC 
CGCCTGGAGG AGGAGCGGGA CAGGAAGCGC CGGGAGCAGG AGCGGAAGCT GGGCCAGATG 
CGCTCCCAGG CCCTGAACCC GGCCGCGTTC TCCCCAGTGA CCGTCGCGCA CACGAAGCCG 
GAGAAACCTT CCACGCTCCA GAGGCCCCAG GACACGGTCA TCCGGGAGCT GCAGCCCCAG 
CAGCAGCCAC GCACCATCGA GCGCCGAGAC TTGCAGTACA TCACGGTCAG CAAAGAGGAG 
CTGTCCTCGG GGGACAGCCT GTCCCCCGAC CCCTGGAAGC GGGACGCCAG GGAGAAGCTG 
GAGAAGCAGC AGCAAATGCA CATCGTGGAC ATGCTGAGCA GGGAGATCCG GGAGCTCCAG 
AGCAAGCCCG AGCGCAGCGC CGAGGACAGC GACCGCCTGC GCAAGCTCAT GCTGGAGTGG 
CAGTTCCAGA AGAGACTGCA GGAGTCCAAG CAGAAGGACG AGGACGACGA GGAGGAGGAG 
GACGACGACG TGGACACCAT GCTGATCATG CAGCGCCTCG AGGCTGAGCG GAGAGCAAGG 
CAGACTGGTC TGCCAGCAAT CTCTGTTCTA GATTTGTTAC AGGACGAGGA GCGGCGGCGG 
CAGCGGCAGC TGGACGACAT GCGCAGGCGG GAAGCGGAGG ACCGCGCCAG GCAGGAGGAG 
GAGAGGACGA AGCGAGACGC CGAAGACAAG AGGCGACAGG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGCTGCT GGAGCCGGAG 
GAGCCGGGCC TGGGCAGACC GCCGCTGCCG CGGGGCTACG AGCTCCCGGC CCCGTCCTCC 
CCGGCGGCTG CTCCTCCTCC CCCACCTCAG CGGACCGCCT CCTCCCTGCA GGCCCCGGCC 
CTGTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG 
GAGGACTGCA GTCTGGCAGG TCAGGACAAG CTCTCCCACA CCGGACCAAA CTCTTCCGTG 
GGATCTTCCG GGGCAGCGAT CGGTGCCCGT GACGGCTCCC GGGACCCGAG AGAGAGGCTG 
GCGAAAAGCC ATGATGCAGA TTTACCTGGA AATTCCGGAG CCCCTGAGAA CTTGACATTC 
AAAGAGCGCC AGCGGCTGTT CTCACAAGGT CAAGATGTGT CCAATAAAGT GAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831489.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687048.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OFb43225
Clone ID Related Accession (Same CDS sequence)	XM_019831489.1
Accession Version	XM_019831489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGAAACA GAAGAATATC GCAGGGGCAC TTGCTTTCTG GATGGCCAAT 
GCATCTGAGC TTCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTTTAGCACA CTTGGTTCAA ATGGCGTTTA AATATCTGGT TCATTGTCTT 
CAGTCAGAAC TGAATAACTA CATGCCAGCT TTTCTGGATG ACCCTGAAGA GAACAGTCTA 
CAAAGACCAA AAATAGATGA CGTCTTGCAC ACGCTCACGG GAGCGATGGC GCTGCTGAGG 
CGCTGCAGGG TCAATGCTGC TCTGACCATC CAGCTTTTCT CTCAGCTCTT CCACTTCATC 
AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCCGAGT CGGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGG CACGTGGAAG CCTGGGCAGA GAAGCAGGGA 
TTAGAGCTGG CAGCCGACTG TCACCTGAGC CGGATAGTGC AGGCGACCAC TTTGCTCACC 
ATGGACAAAT ACGCTCCTGA GGACACCCCA AATATCAACA GCACCTGCTT TAAGTTAAAT 
TCGCTGCAGC TGCAGGCCTT GCTACAGAAT TACCACTGTG CGCCCGACGA GCCCTTTGTC 
CCCACGGACC TGATAGAGAA TGTCGTGGCC GTGGCTGAAA ACACGGCCGA CGAGCTGGCA 
CGCAGTGATG GGCGGGAGGT GCAGCTGGAA GAGGACCCTG ATCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGCTATTC TTGTGACGTC GTCAGGAACG TTCCAAACGG TTTACAAGAA 
TTTCTGGACC CTCTGTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCATGC ACGTTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCATCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GCGGAAAGAA CCTGAAATAA TCACTGTGAC TCTCAAAAAG 
CAAAACGGAA TGGGCCTCAG CATTGTCGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA 
ATCTATGTCA AGTCTGTCGT AAAAGGAGGT GCTGCAGATG TGGATGGACG CCTAGCCGCA 
GGTGACCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCA 
GCAGAACTCA TGACAAGGAC CAGTTCCGTG GTGACACTGG AAGTAGCCAA GCAAGGAGCG 
ATCTACCACG GCCTGGCCAC CCTGCTCAAC CAGCCGTCCC CGATGATGCA GAGAGTTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTCGAGCT CTATAACAAT 
TCAGCTCAAA ACGGGTCACC CGAGAGCCCT CAGCTGCCTT GGGCAGAATA CAGCGAACCG 
AAGAAGCTCC CTGGCGATGA CAGGCTGATG AAGAACCGGG CTGACCACCG CTCCAGCCCC 
AACGTGGCAA ATCAGCCCCC CAGTCCCGGA GGGAAAAGCG CGTACGCCCC TGGAGCAGCC 
GCTAAGATCA CGTCCGTCTC CACCGGAAAC CTCTGCACTG AGGAGCAGAC CCCTCCGCCT 
CGACCTGAGG CCTACCCCAT CCCCACCCAG ACATACACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGCTCCC CCTCAGAACC AGTGGCCCAA TTACGAGGAG 
AAGCCGCACG TGCACACAGA TGGTCATCAG TCCGGCCCCG CAATGCAGCG CGTCACCCGC 
TCCCAGGAGG AACTTCGGGA GGATAAAGCT TACCAACTCG AGCGGCATCG GCTGGAGGCG 
GTGCTGGATC GCAAGTCCGA CAGCGACGTG TGGATCCAGC CGAGCTCCTC GGTGGGCTCC 
AGCACGTCCA GCCAGGAGCA CCTGGACCCC TGCTCCAAGT CGGGCACCCC CGCCTCCACG 
CTGACCAAGA GCGGCCCCGG GCGCTGGAAG ACGCCAGCCT CCGCGCAGCC CCTGCCGGCG 
GCCCCCCCGC CCGCGCAGAC AGACCTGCCC CCACCACCCC CGCCGCCCCC CGCGCACTAC 
GCCGGAGACT TCGACGGGAT CCCCGTGGAC CTGCCCCTCC CGCCCCCTCC CAGCGGCCAG 
GCGGCGGCGG AGCGGAGGAA ACGGGAGGAG CAGCAGCGCT GGTACGAGAA GGAGAAGGCC 
CGCCTGGAGG AGGAGCGGGA CAGGAAGCGC CGGGAGCAGG AGCGGAAGCT GGGCCAGATG 
CGCTCCCAGG CCCTGAACCC GGCCGCGTTC TCCCCAGTGA CCGTCGCGCA CACGAAGCCG 
GAGAAACCTT CCACGCTCCA GAGGCCCCAG GACACGGTCA TCCGGGAGCT GCAGCCCCAG 
CAGCAGCCAC GCACCATCGA GCGCCGAGAC TTGCAGTACA TCACGGTCAG CAAAGAGGAG 
CTGTCCTCGG GGGACAGCCT GTCCCCCGAC CCCTGGAAGC GGGACGCCAG GGAGAAGCTG 
GAGAAGCAGC AGCAAATGCA CATCGTGGAC ATGCTGAGCA GGGAGATCCG GGAGCTCCAG 
AGCAAGCCCG AGCGCAGCGC CGAGGACAGC GACCGCCTGC GCAAGCTCAT GCTGGAGTGG 
CAGTTCCAGA AGAGACTGCA GGAGTCCAAG CAGAAGGACG AGGACGACGA GGAGGAGGAG 
GACGACGACG TGGACACCAT GCTGATCATG CAGCGCCTCG AGGCTGAGCG GAGAGCAAGG 
CAGACTGGTC TGCCAGCAAT CTCTGTTCTA GATTTGTTAC AGGACGAGGA GCGGCGGCGG 
CAGCGGCAGC TGGACGACAT GCGCAGGCGG GAAGCGGAGG ACCGCGCCAG GCAGGAGGAG 
GAGAGGACGA AGCGAGACGC CGAAGACAAG AGGCGACAGG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGCTGCT GGAGCCGGAG 
GAGCCGGGCC TGGGCAGACC GCCGCTGCCG CGGGGCTACG AGCTCCCGGC CCCGTCCTCC 
CCGGCGGCTG CTCCTCCTCC CCCACCTCAG CGGACCGCCT CCTCCCTGCA GGCCCCGGCC 
CTGTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG 
GAGGACTGCA GTCTGGCAGG TCAGGACAAG CTCTCCCACA CCGGACCAAA CTCTTCCGTG 
GGATCTTCCG GGGCAGCGAT CGGTGCCCGT GACGGCTCCC GGGACCCGAG AGAGAGGCTG 
GCGAAAAGCC ATGATGCAGA TTTACCTGGA AATTCCGGAG CCCCTGAGAA CTTGACATTC 
AAAGAGCGCC AGCGGCTGTT CTCACAAGGT CAAGATGTGT CCAATAAAGT GAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687048.1
CDS	22..5403
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831489.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831489.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGAAACA GAAGAATATC GCAGGGGCAC TTGCTTTCTG GATGGCCAAT 
GCATCTGAGC TTCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTTTAGCACA CTTGGTTCAA ATGGCGTTTA AATATCTGGT TCATTGTCTT 
CAGTCAGAAC TGAATAACTA CATGCCAGCT TTTCTGGATG ACCCTGAAGA GAACAGTCTA 
CAAAGACCAA AAATAGATGA CGTCTTGCAC ACGCTCACGG GAGCGATGGC GCTGCTGAGG 
CGCTGCAGGG TCAATGCTGC TCTGACCATC CAGCTTTTCT CTCAGCTCTT CCACTTCATC 
AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCCGAGT CGGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGG CACGTGGAAG CCTGGGCAGA GAAGCAGGGA 
TTAGAGCTGG CAGCCGACTG TCACCTGAGC CGGATAGTGC AGGCGACCAC TTTGCTCACC 
ATGGACAAAT ACGCTCCTGA GGACACCCCA AATATCAACA GCACCTGCTT TAAGTTAAAT 
TCGCTGCAGC TGCAGGCCTT GCTACAGAAT TACCACTGTG CGCCCGACGA GCCCTTTGTC 
CCCACGGACC TGATAGAGAA TGTCGTGGCC GTGGCTGAAA ACACGGCCGA CGAGCTGGCA 
CGCAGTGATG GGCGGGAGGT GCAGCTGGAA GAGGACCCTG ATCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGCTATTC TTGTGACGTC GTCAGGAACG TTCCAAACGG TTTACAAGAA 
TTTCTGGACC CTCTGTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCATGC ACGTTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCATCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GCGGAAAGAA CCTGAAATAA TCACTGTGAC TCTCAAAAAG 
CAAAACGGAA TGGGCCTCAG CATTGTCGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA 
ATCTATGTCA AGTCTGTCGT AAAAGGAGGT GCTGCAGATG TGGATGGACG CCTAGCCGCA 
GGTGACCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCA 
GCAGAACTCA TGACAAGGAC CAGTTCCGTG GTGACACTGG AAGTAGCCAA GCAAGGAGCG 
ATCTACCACG GCCTGGCCAC CCTGCTCAAC CAGCCGTCCC CGATGATGCA GAGAGTTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTCGAGCT CTATAACAAT 
TCAGCTCAAA ACGGGTCACC CGAGAGCCCT CAGCTGCCTT GGGCAGAATA CAGCGAACCG 
AAGAAGCTCC CTGGCGATGA CAGGCTGATG AAGAACCGGG CTGACCACCG CTCCAGCCCC 
AACGTGGCAA ATCAGCCCCC CAGTCCCGGA GGGAAAAGCG CGTACGCCCC TGGAGCAGCC 
GCTAAGATCA CGTCCGTCTC CACCGGAAAC CTCTGCACTG AGGAGCAGAC CCCTCCGCCT 
CGACCTGAGG CCTACCCCAT CCCCACCCAG ACATACACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGCTCCC CCTCAGAACC AGTGGCCCAA TTACGAGGAG 
AAGCCGCACG TGCACACAGA TGGTCATCAG TCCGGCCCCG CAATGCAGCG CGTCACCCGC 
TCCCAGGAGG AACTTCGGGA GGATAAAGCT TACCAACTCG AGCGGCATCG GCTGGAGGCG 
GTGCTGGATC GCAAGTCCGA CAGCGACGTG TGGATCCAGC CGAGCTCCTC GGTGGGCTCC 
AGCACGTCCA GCCAGGAGCA CCTGGACCCC TGCTCCAAGT CGGGCACCCC CGCCTCCACG 
CTGACCAAGA GCGGCCCCGG GCGCTGGAAG ACGCCAGCCT CCGCGCAGCC CCTGCCGGCG 
GCCCCCCCGC CCGCGCAGAC AGACCTGCCC CCACCACCCC CGCCGCCCCC CGCGCACTAC 
GCCGGAGACT TCGACGGGAT CCCCGTGGAC CTGCCCCTCC CGCCCCCTCC CAGCGGCCAG 
GCGGCGGCGG AGCGGAGGAA ACGGGAGGAG CAGCAGCGCT GGTACGAGAA GGAGAAGGCC 
CGCCTGGAGG AGGAGCGGGA CAGGAAGCGC CGGGAGCAGG AGCGGAAGCT GGGCCAGATG 
CGCTCCCAGG CCCTGAACCC GGCCGCGTTC TCCCCAGTGA CCGTCGCGCA CACGAAGCCG 
GAGAAACCTT CCACGCTCCA GAGGCCCCAG GACACGGTCA TCCGGGAGCT GCAGCCCCAG 
CAGCAGCCAC GCACCATCGA GCGCCGAGAC TTGCAGTACA TCACGGTCAG CAAAGAGGAG 
CTGTCCTCGG GGGACAGCCT GTCCCCCGAC CCCTGGAAGC GGGACGCCAG GGAGAAGCTG 
GAGAAGCAGC AGCAAATGCA CATCGTGGAC ATGCTGAGCA GGGAGATCCG GGAGCTCCAG 
AGCAAGCCCG AGCGCAGCGC CGAGGACAGC GACCGCCTGC GCAAGCTCAT GCTGGAGTGG 
CAGTTCCAGA AGAGACTGCA GGAGTCCAAG CAGAAGGACG AGGACGACGA GGAGGAGGAG 
GACGACGACG TGGACACCAT GCTGATCATG CAGCGCCTCG AGGCTGAGCG GAGAGCAAGG 
CAGACTGGTC TGCCAGCAAT CTCTGTTCTA GATTTGTTAC AGGACGAGGA GCGGCGGCGG 
CAGCGGCAGC TGGACGACAT GCGCAGGCGG GAAGCGGAGG ACCGCGCCAG GCAGGAGGAG 
GAGAGGACGA AGCGAGACGC CGAAGACAAG AGGCGACAGG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGCTGCT GGAGCCGGAG 
GAGCCGGGCC TGGGCAGACC GCCGCTGCCG CGGGGCTACG AGCTCCCGGC CCCGTCCTCC 
CCGGCGGCTG CTCCTCCTCC CCCACCTCAG CGGACCGCCT CCTCCCTGCA GGCCCCGGCC 
CTGTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG 
GAGGACTGCA GTCTGGCAGG TCAGGACAAG CTCTCCCACA CCGGACCAAA CTCTTCCGTG 
GGATCTTCCG GGGCAGCGAT CGGTGCCCGT GACGGCTCCC GGGACCCGAG AGAGAGGCTG 
GCGAAAAGCC ATGATGCAGA TTTACCTGGA AATTCCGGAG CCCCTGAGAA CTTGACATTC 
AAAGAGCGCC AGCGGCTGTT CTCACAAGGT CAAGATGTGT CCAATAAAGT GAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831490.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687049.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OFb43226
Clone ID Related Accession (Same CDS sequence)	XM_019831490.1
Accession Version	XM_019831490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGCAGACTG GTCTGCCAGC AATCTCTGTT CTAGATTTGG ACGAGGAGCG GCGGCGGCAG 
CGGCAGCTGG ACGACATGCG CAGGCGGGAA GCGGAGGACC GCGCCAGGCA GGAGGAGGAG 
AGGACGAAGC GAGACGCCGA AGACAAGAGG CGACAGGAAG AAGGTTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGCGGAG GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG 
CCGGGCCTGG GCAGACCGCC GCTGCCGCGG GGCTACGAGC TCCCGGCCCC GTCCTCCCCG 
GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG 
TCCCCAGACT CCCTGTACAC CGCCAGGCTC GTTGCGTACC ACGAGGAGGA GGAGGAGGAG 
GACTGCAGTC TGGCAGGTCA GGACAAGCTC TCCCACACCG GACCAAACTC TTCCGTGGGA 
TCTTCCGGGG CAGCGATCGG TGCCCGTGAC GGCTCCCGGG ACCCGAGAGA GAGGCTGGCG 
AAAAGCCATG ATGCAGATTT ACCTGGAAAT TCCGGAGCCC CTGAGAACTT GACATTCAAA 
GAGCGCCAGC GGCTGTTCTC ACAAGGTCAA GATGTGTCCA ATAAAGTGAA AGCCTCTCGT 
AAATTAACAG AACTGGAAAA CGAACTGAAC ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687049.1
CDS	22..5400
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831490.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831490.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGCAGACTG GTCTGCCAGC AATCTCTGTT CTAGATTTGG ACGAGGAGCG GCGGCGGCAG 
CGGCAGCTGG ACGACATGCG CAGGCGGGAA GCGGAGGACC GCGCCAGGCA GGAGGAGGAG 
AGGACGAAGC GAGACGCCGA AGACAAGAGG CGACAGGAAG AAGGTTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGCGGAG GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG 
CCGGGCCTGG GCAGACCGCC GCTGCCGCGG GGCTACGAGC TCCCGGCCCC GTCCTCCCCG 
GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG 
TCCCCAGACT CCCTGTACAC CGCCAGGCTC GTTGCGTACC ACGAGGAGGA GGAGGAGGAG 
GACTGCAGTC TGGCAGGTCA GGACAAGCTC TCCCACACCG GACCAAACTC TTCCGTGGGA 
TCTTCCGGGG CAGCGATCGG TGCCCGTGAC GGCTCCCGGG ACCCGAGAGA GAGGCTGGCG 
AAAAGCCATG ATGCAGATTT ACCTGGAAAT TCCGGAGCCC CTGAGAACTT GACATTCAAA 
GAGCGCCAGC GGCTGTTCTC ACAAGGTCAA GATGTGTCCA ATAAAGTGAA AGCCTCTCGT 
AAATTAACAG AACTGGAAAA CGAACTGAAC ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831491.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687050.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

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CloneID	OFb43227
Clone ID Related Accession (Same CDS sequence)	XM_019831491.1
Accession Version	XM_019831491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGAAA 
CAGAAGAATA TCGCAGGGGC ACTTGCTTTC TGGATGGCCA ATGCATCTGA GCTTCTCAAC 
TTCATTAAGC AGGACCGAGA TCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTTTAGCA 
CACTTGGTTC AAATGGCGTT TAAATATCTG GTTCATTGTC TTCAGTCAGA ACTGAATAAC 
TACATGCCAG CTTTTCTGGA TGACCCTGAA GAGAACAGTC TACAAAGACC AAAAATAGAT 
GACGTCTTGC ACACGCTCAC GGGAGCGATG GCGCTGCTGA GGCGCTGCAG GGTCAATGCT 
GCTCTGACCA TCCAGCTTTT CTCTCAGCTC TTCCACTTCA TCAACATGTG GCTCTTCAAC 
AGATTGGTGA CCGACCCCGA GTCGGGACTG TGCTCTCACT ACTGGGGGGC AATCATCCGC 
CAGCAGCTGG GGCACGTGGA AGCCTGGGCA GAGAAGCAGG GATTAGAGCT GGCAGCCGAC 
TGTCACCTGA GCCGGATAGT GCAGGCGACC ACTTTGCTCA CCATGGACAA ATACGCTCCT 
GAGGACACCC CAAATATCAA CAGCACCTGC TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC 
TTGCTACAGA ATTACCACTG TGCGCCCGAC GAGCCCTTTG TCCCCACGGA CCTGATAGAG 
AATGTCGTGG CCGTGGCTGA AAACACGGCC GACGAGCTGG CACGCAGTGA TGGGCGGGAG 
GTGCAGCTGG AAGAGGACCC TGATCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGCTAT 
TCTTGTGACG TCGTCAGGAA CGTTCCAAAC GGTTTACAAG AATTTCTGGA CCCTCTGTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAT GCACGTTCAC CAGGTACTTG GACGATATAT 
TTTGAAGGTG CAGATTATGA GAGCCATCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGCGGAAAG AACCTGAAAT AATCACTGTG ACTCTCAAAA AGCAAAACGG AATGGGCCTC 
AGCATTGTCG CAGCAAAGGG TGCTGGTCAA GATAAACTTG GAATCTATGT CAAGTCTGTC 
GTAAAAGGAG GTGCTGCAGA TGTGGATGGA CGCCTAGCCG CAGGTGACCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT AGGACTCTCT CAAGAAAGGG CAGCAGAACT CATGACAAGG 
ACCAGTTCCG TGGTGACACT GGAAGTAGCC AAGCAAGGAG CGATCTACCA CGGCCTGGCC 
ACCCTGCTCA ACCAGCCGTC CCCGATGATG CAGAGAGTTT CAGACCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTCGAG CTCTATAACA ATTCAGCTCA AAACGGGTCA 
CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA TACAGCGAAC CGAAGAAGCT CCCTGGCGAT 
GACAGGCTGA TGAAGAACCG GGCTGACCAC CGCTCCAGCC CCAACGTGGC AAATCAGCCC 
CCCAGTCCCG GAGGGAAAAG CGCGTACGCC CCTGGAGCAG CCGCTAAGAT CACGTCCGTC 
TCCACCGGAA ACCTCTGCAC TGAGGAGCAG ACCCCTCCGC CTCGACCTGA GGCCTACCCC 
ATCCCCACCC AGACATACAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC AATTACGAGG AGAAGCCGCA CGTGCACACA 
GATGGTCATC AGTCCGGCCC CGCAATGCAG CGCGTCACCC GCTCCCAGGA GGAACTTCGG 
GAGGATAAAG CTTACCAACT CGAGCGGCAT CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC 
GACAGCGACG TGTGGATCCA GCCGAGCTCC TCGGTGGGCT CCAGCACGTC CAGCCAGGAG 
CACCTGGACC CCTGCTCCAA GTCGGGCACC CCCGCCTCCA CGCTGACCAA GAGCGGCCCC 
GGGCGCTGGA AGACGCCAGC CTCCGCGCAG CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG 
ACAGACCTGC CCCCACCACC CCCGCCGCCC CCCGCGCACT ACGCCGGAGA CTTCGACGGG 
ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG 
AAACGGGAGG AGCAGCAGCG CTGGTACGAG AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG 
GACAGGAAGC GCCGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC 
CCGGCCGCGT TCTCCCCAGT GACCGTCGCG CACACGAAGC CGGAGAAACC TTCCACGCTC 
CAGAGGCCCC AGGACACGGT CATCCGGGAG CTGCAGCCCC AGCAGCAGCC ACGCACCATC 
GAGCGCCGAG ACTTGCAGTA CATCACGGTC AGCAAAGAGG AGCTGTCCTC GGGGGACAGC 
CTGTCCCCCG ACCCCTGGAA GCGGGACGCC AGGGAGAAGC TGGAGAAGCA GCAGCAAATG 
CACATCGTGG ACATGCTGAG CAGGGAGATC CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC 
GCCGAGGACA GCGACCGCCT GCGCAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTG 
CAGGAGTCCA AGCAGAAGGA CGAGGACGAC GAGGAGGAGG AGGACGACGA CGTGGACACC 
ATGCTGATCA TGCAGCGCCT CGAGGCTGAG CGGAGAGCAA GGCAGACTGG TCTGCCAGCA 
ATCTCTGTTC TAGATTTGTT ACAGGACGAG GAGCGGCGGC GGCAGCGGCA GCTGGACGAC 
ATGCGCAGGC GGGAAGCGGA GGACCGCGCC AGGCAGGAGG AGGAGAGGAC GAAGCGAGAC 
GCCGAAGACA AGAGGCGACA GGAAGAAGGT TATTACAGCC GCCTGGAAGC CGAGAGGCGC 
AGACAGCACG CGGAGGCCGA GCGCAGGCTG CTGGAGCCGG AGGAGCCGGG CCTGGGCAGA 
CCGCCGCTGC CGCGGGGCTA CGAGCTCCCG GCCCCGTCCT CCCCGGCGGC TGCTCCTCCT 
CCCCCACCTC AGCGGACCGC CTCCTCCCTG CAGGCCCCGG CCCTGTCCCC AGACTCCCTG 
TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGGACTG CAGTCTGGCA 
GGTCAGGACA AGCTCTCCCA CACCGGACCA AACTCTTCCG TGGGATCTTC CGGGGCAGCG 
ATCGGTGCCC GTGACGGCTC CCGGGACCCG AGAGAGAGGC TGGCGAAAAG CCATGATGCA 
GATTTACCTG GAAATTCCGG AGCCCCTGAG AACTTGACAT TCAAAGAGCG CCAGCGGCTG 
TTCTCACAAG GTCAAGATGT GTCCAATAAA GTGAAAGCCT CTCGTAAATT AACAGAACTG 
GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687050.1
CDS	22..5385
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831491.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831491.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGAAA 
CAGAAGAATA TCGCAGGGGC ACTTGCTTTC TGGATGGCCA ATGCATCTGA GCTTCTCAAC 
TTCATTAAGC AGGACCGAGA TCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTTTAGCA 
CACTTGGTTC AAATGGCGTT TAAATATCTG GTTCATTGTC TTCAGTCAGA ACTGAATAAC 
TACATGCCAG CTTTTCTGGA TGACCCTGAA GAGAACAGTC TACAAAGACC AAAAATAGAT 
GACGTCTTGC ACACGCTCAC GGGAGCGATG GCGCTGCTGA GGCGCTGCAG GGTCAATGCT 
GCTCTGACCA TCCAGCTTTT CTCTCAGCTC TTCCACTTCA TCAACATGTG GCTCTTCAAC 
AGATTGGTGA CCGACCCCGA GTCGGGACTG TGCTCTCACT ACTGGGGGGC AATCATCCGC 
CAGCAGCTGG GGCACGTGGA AGCCTGGGCA GAGAAGCAGG GATTAGAGCT GGCAGCCGAC 
TGTCACCTGA GCCGGATAGT GCAGGCGACC ACTTTGCTCA CCATGGACAA ATACGCTCCT 
GAGGACACCC CAAATATCAA CAGCACCTGC TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC 
TTGCTACAGA ATTACCACTG TGCGCCCGAC GAGCCCTTTG TCCCCACGGA CCTGATAGAG 
AATGTCGTGG CCGTGGCTGA AAACACGGCC GACGAGCTGG CACGCAGTGA TGGGCGGGAG 
GTGCAGCTGG AAGAGGACCC TGATCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGCTAT 
TCTTGTGACG TCGTCAGGAA CGTTCCAAAC GGTTTACAAG AATTTCTGGA CCCTCTGTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAT GCACGTTCAC CAGGTACTTG GACGATATAT 
TTTGAAGGTG CAGATTATGA GAGCCATCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGCGGAAAG AACCTGAAAT AATCACTGTG ACTCTCAAAA AGCAAAACGG AATGGGCCTC 
AGCATTGTCG CAGCAAAGGG TGCTGGTCAA GATAAACTTG GAATCTATGT CAAGTCTGTC 
GTAAAAGGAG GTGCTGCAGA TGTGGATGGA CGCCTAGCCG CAGGTGACCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT AGGACTCTCT CAAGAAAGGG CAGCAGAACT CATGACAAGG 
ACCAGTTCCG TGGTGACACT GGAAGTAGCC AAGCAAGGAG CGATCTACCA CGGCCTGGCC 
ACCCTGCTCA ACCAGCCGTC CCCGATGATG CAGAGAGTTT CAGACCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTCGAG CTCTATAACA ATTCAGCTCA AAACGGGTCA 
CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA TACAGCGAAC CGAAGAAGCT CCCTGGCGAT 
GACAGGCTGA TGAAGAACCG GGCTGACCAC CGCTCCAGCC CCAACGTGGC AAATCAGCCC 
CCCAGTCCCG GAGGGAAAAG CGCGTACGCC CCTGGAGCAG CCGCTAAGAT CACGTCCGTC 
TCCACCGGAA ACCTCTGCAC TGAGGAGCAG ACCCCTCCGC CTCGACCTGA GGCCTACCCC 
ATCCCCACCC AGACATACAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC AATTACGAGG AGAAGCCGCA CGTGCACACA 
GATGGTCATC AGTCCGGCCC CGCAATGCAG CGCGTCACCC GCTCCCAGGA GGAACTTCGG 
GAGGATAAAG CTTACCAACT CGAGCGGCAT CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC 
GACAGCGACG TGTGGATCCA GCCGAGCTCC TCGGTGGGCT CCAGCACGTC CAGCCAGGAG 
CACCTGGACC CCTGCTCCAA GTCGGGCACC CCCGCCTCCA CGCTGACCAA GAGCGGCCCC 
GGGCGCTGGA AGACGCCAGC CTCCGCGCAG CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG 
ACAGACCTGC CCCCACCACC CCCGCCGCCC CCCGCGCACT ACGCCGGAGA CTTCGACGGG 
ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG 
AAACGGGAGG AGCAGCAGCG CTGGTACGAG AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG 
GACAGGAAGC GCCGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC 
CCGGCCGCGT TCTCCCCAGT GACCGTCGCG CACACGAAGC CGGAGAAACC TTCCACGCTC 
CAGAGGCCCC AGGACACGGT CATCCGGGAG CTGCAGCCCC AGCAGCAGCC ACGCACCATC 
GAGCGCCGAG ACTTGCAGTA CATCACGGTC AGCAAAGAGG AGCTGTCCTC GGGGGACAGC 
CTGTCCCCCG ACCCCTGGAA GCGGGACGCC AGGGAGAAGC TGGAGAAGCA GCAGCAAATG 
CACATCGTGG ACATGCTGAG CAGGGAGATC CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC 
GCCGAGGACA GCGACCGCCT GCGCAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTG 
CAGGAGTCCA AGCAGAAGGA CGAGGACGAC GAGGAGGAGG AGGACGACGA CGTGGACACC 
ATGCTGATCA TGCAGCGCCT CGAGGCTGAG CGGAGAGCAA GGCAGACTGG TCTGCCAGCA 
ATCTCTGTTC TAGATTTGTT ACAGGACGAG GAGCGGCGGC GGCAGCGGCA GCTGGACGAC 
ATGCGCAGGC GGGAAGCGGA GGACCGCGCC AGGCAGGAGG AGGAGAGGAC GAAGCGAGAC 
GCCGAAGACA AGAGGCGACA GGAAGAAGGT TATTACAGCC GCCTGGAAGC CGAGAGGCGC 
AGACAGCACG CGGAGGCCGA GCGCAGGCTG CTGGAGCCGG AGGAGCCGGG CCTGGGCAGA 
CCGCCGCTGC CGCGGGGCTA CGAGCTCCCG GCCCCGTCCT CCCCGGCGGC TGCTCCTCCT 
CCCCCACCTC AGCGGACCGC CTCCTCCCTG CAGGCCCCGG CCCTGTCCCC AGACTCCCTG 
TACACCGCCA GGCTCGTTGC GTACCACGAG GAGGAGGAGG AGGAGGACTG CAGTCTGGCA 
GGTCAGGACA AGCTCTCCCA CACCGGACCA AACTCTTCCG TGGGATCTTC CGGGGCAGCG 
ATCGGTGCCC GTGACGGCTC CCGGGACCCG AGAGAGAGGC TGGCGAAAAG CCATGATGCA 
GATTTACCTG GAAATTCCGG AGCCCCTGAG AACTTGACAT TCAAAGAGCG CCAGCGGCTG 
TTCTCACAAG GTCAAGATGT GTCCAATAAA GTGAAAGCCT CTCGTAAATT AACAGAACTG 
GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831492.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687051.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OFb43228
Clone ID Related Accession (Same CDS sequence)	XM_019831492.1
Accession Version	XM_019831492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGCAGACTG GTCTGCCAGC AATCTCTGTT CTAGATTTGT TACAGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGACCAAAC TCTTCCGTGG GATCTTCCGG GGCAGCGATC 
GGTGCCCGTG ACGGCTCCCG GGACCCGAGA GAGAGGCTGG CGAAAAGCCA TGATGCAGAT 
TTACCTGGAA ATTCCGGAGC CCCTGAGAAC TTGACATTCA AAGAGCGCCA GCGGCTGTTC 
TCACAAGGTC AAGATGTGTC CAATAAAGTG AAAGCCTCTC GTAAATTAAC AGAACTGGAA 
AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687051.1
CDS	22..5382
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831492.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831492.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGCAGACTG GTCTGCCAGC AATCTCTGTT CTAGATTTGT TACAGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGACCAAAC TCTTCCGTGG GATCTTCCGG GGCAGCGATC 
GGTGCCCGTG ACGGCTCCCG GGACCCGAGA GAGAGGCTGG CGAAAAGCCA TGATGCAGAT 
TTACCTGGAA ATTCCGGAGC CCCTGAGAAC TTGACATTCA AAGAGCGCCA GCGGCTGTTC 
TCACAAGGTC AAGATGTGTC CAATAAAGTG AAAGCCTCTC GTAAATTAAC AGAACTGGAA 
AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831493.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687052.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

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CloneID	OFb43229
Clone ID Related Accession (Same CDS sequence)	XM_019831493.1
Accession Version	XM_019831493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGTTACAGG ACGAGGAGCG GCGGCGGCAG CGGCAGCTGG ACGACATGCG CAGGCGGGAA 
GCGGAGGACC GCGCCAGGCA GGAGGAGGAG AGGACGAAGC GAGACGCCGA AGACAAGAGG 
CGACAGGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG CCGGGCCTGG GCAGACCGCC GCTGCCGCGG 
GGCTACGAGC TCCCGGCCCC GTCCTCCCCG GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG 
ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG TCCCCAGACT CCCTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGA GGAGGAGGAG GACTGCAGTC TGGCAGGTCA GGACAAGCTC 
TCCCACACCG GACCAAACTC TTCCGTGGGA TCTTCCGGGG CAGCGATCGG TGCCCGTGAC 
GGCTCCCGGG ACCCGAGAGA GAGGCTGGCG AAAAGCCATG ATGCAGATTT ACCTGGAAAT 
TCCGGAGCCC CTGAGAACTT GACATTCAAA GAGCGCCAGC GGCTGTTCTC ACAAGGTCAA 
GATGTGTCCA ATAAAGTGAA AGCCTCTCGT AAATTAACAG AACTGGAAAA CGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687052.1
CDS	22..5370
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831493.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831493.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGTTACAGG ACGAGGAGCG GCGGCGGCAG CGGCAGCTGG ACGACATGCG CAGGCGGGAA 
GCGGAGGACC GCGCCAGGCA GGAGGAGGAG AGGACGAAGC GAGACGCCGA AGACAAGAGG 
CGACAGGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG CCGGGCCTGG GCAGACCGCC GCTGCCGCGG 
GGCTACGAGC TCCCGGCCCC GTCCTCCCCG GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG 
ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG TCCCCAGACT CCCTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGA GGAGGAGGAG GACTGCAGTC TGGCAGGTCA GGACAAGCTC 
TCCCACACCG GACCAAACTC TTCCGTGGGA TCTTCCGGGG CAGCGATCGG TGCCCGTGAC 
GGCTCCCGGG ACCCGAGAGA GAGGCTGGCG AAAAGCCATG ATGCAGATTT ACCTGGAAAT 
TCCGGAGCCC CTGAGAACTT GACATTCAAA GAGCGCCAGC GGCTGTTCTC ACAAGGTCAA 
GATGTGTCCA ATAAAGTGAA AGCCTCTCGT AAATTAACAG AACTGGAAAA CGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831494.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687053.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OFb43230
Clone ID Related Accession (Same CDS sequence)	XM_019831494.1
Accession Version	XM_019831494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGGACGAGG AGCGGCGGCG GCAGCGGCAG CTGGACGACA TGCGCAGGCG GGAAGCGGAG 
GACCGCGCCA GGCAGGAGGA GGAGAGGACG AAGCGAGACG CCGAAGACAA GAGGCGACAG 
GAAGAAGGTT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGC GGAGGCCGAG 
CGCAGGCTGC TGGAGCCGGA GGAGCCGGGC CTGGGCAGAC CGCCGCTGCC GCGGGGCTAC 
GAGCTCCCGG CCCCGTCCTC CCCGGCGGCT GCTCCTCCTC CCCCACCTCA GCGGACCGCC 
TCCTCCCTGC AGGCCCCGGC CCTGTCCCCA GACTCCCTGT ACACCGCCAG GCTCGTTGCG 
TACCACGAGG AGGAGGAGGA GGAGGACTGC AGTCTGGCAG GTCAGGACAA GCTCTCCCAC 
ACCGGACCAA ACTCTTCCGT GGGATCTTCC GGGGCAGCGA TCGGTGCCCG TGACGGCTCC 
CGGGACCCGA GAGAGAGGCT GGCGAAAAGC CATGATGCAG ATTTACCTGG AAATTCCGGA 
GCCCCTGAGA ACTTGACATT CAAAGAGCGC CAGCGGCTGT TCTCACAAGG TCAAGATGTG 
TCCAATAAAG TGAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687053.1
CDS	22..5364
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831494.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831494.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGGACGAGG AGCGGCGGCG GCAGCGGCAG CTGGACGACA TGCGCAGGCG GGAAGCGGAG 
GACCGCGCCA GGCAGGAGGA GGAGAGGACG AAGCGAGACG CCGAAGACAA GAGGCGACAG 
GAAGAAGGTT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGC GGAGGCCGAG 
CGCAGGCTGC TGGAGCCGGA GGAGCCGGGC CTGGGCAGAC CGCCGCTGCC GCGGGGCTAC 
GAGCTCCCGG CCCCGTCCTC CCCGGCGGCT GCTCCTCCTC CCCCACCTCA GCGGACCGCC 
TCCTCCCTGC AGGCCCCGGC CCTGTCCCCA GACTCCCTGT ACACCGCCAG GCTCGTTGCG 
TACCACGAGG AGGAGGAGGA GGAGGACTGC AGTCTGGCAG GTCAGGACAA GCTCTCCCAC 
ACCGGACCAA ACTCTTCCGT GGGATCTTCC GGGGCAGCGA TCGGTGCCCG TGACGGCTCC 
CGGGACCCGA GAGAGAGGCT GGCGAAAAGC CATGATGCAG ATTTACCTGG AAATTCCGGA 
GCCCCTGAGA ACTTGACATT CAAAGAGCGC CAGCGGCTGT TCTCACAAGG TCAAGATGTG 
TCCAATAAAG TGAAAGCCTC TCGTAAATTA ACAGAACTGG AAAACGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831495.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687054.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

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CloneID	OFb43231
Clone ID Related Accession (Same CDS sequence)	XM_019831495.1
Accession Version	XM_019831495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGATGGTT CTGACTCCAG GGACAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTCGGGA CAGAGAAGTT CGAGGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGAGTTGTCA CCGTGACTCC GAGAAGCATG GACGCCGAGA CGTACGTGGA AGGACAGCGC 
ATCTCCGAGA CAACCATGCT GCAGAGTGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCAGTGGCCA AGAGACCTGT GGATGGAGGC 
CTGACGGCCA AGGGCCCCAG ACAGAAGCCT GGAATTGTTC AGGAGACAAC TTTCGATTTG 
GGGGGAGATG TTCACAGCGG GGCAGCGCTG CCAGCGAGCA AGAGCGCCAC CAGGCTGGAC 
GGCGACAGGG CACTGCCTGC CCCCAGCACG GCCGAGCGCG GGACAGTGAA GCCGATGCTG 
AGACTGGAGC AGCAGGACTA CCGCCGGCAA GAAAACAGAA CTCAGGATGC TCTTGGGCCT 
GAACTCATAC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC ATTTTTATCT 
GCCATTATAA ATTATACTAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTA 
TTATATATGG CATGTCGGTA TGTATTGTCG AGCCAGTACA GACCCGATGT CAGTCCTACA 
GAGCGTACTC ACAAAGTGAT TGCCATAGTC AACAAGATGG TGAGCATGAT GGAGGGGGTG 
ATTCAGGAAG TAGACCAGGT TGATCAGAAA CAGAAGAATA TCGCAGGGGC ACTTGCTTTC 
TGGATGGCCA ATGCATCTGA GCTTCTCAAC TTCATTAAGC AGGACCGAGA TCTTAGTCGA 
ATCACATTGG ATGCCCAAGA TGTTTTAGCA CACTTGGTTC AAATGGCGTT TAAATATCTG 
GTTCATTGTC TTCAGTCAGA ACTGAATAAC TACATGCCAG CTTTTCTGGA TGACCCTGAA 
GAGAACAGTC TACAAAGACC AAAAATAGAT GACGTCTTGC ACACGCTCAC GGGAGCGATG 
GCGCTGCTGA GGCGCTGCAG GGTCAATGCT GCTCTGACCA TCCAGCTTTT CTCTCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AGATTGGTGA CCGACCCCGA GTCGGGACTG 
TGCTCTCACT ACTGGGGGGC AATCATCCGC CAGCAGCTGG GGCACGTGGA AGCCTGGGCA 
GAGAAGCAGG GATTAGAGCT GGCAGCCGAC TGTCACCTGA GCCGGATAGT GCAGGCGACC 
ACTTTGCTCA CCATGGACAA ATACGCTCCT GAGGACACCC CAAATATCAA CAGCACCTGC 
TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC TTGCTACAGA ATTACCACTG TGCGCCCGAC 
GAGCCCTTTG TCCCCACGGA CCTGATAGAG AATGTCGTGG CCGTGGCTGA AAACACGGCC 
GACGAGCTGG CACGCAGTGA TGGGCGGGAG GTGCAGCTGG AAGAGGACCC TGATCTGCAG 
CTGCCTTTTC TTTTGCCGGA AGACGGCTAT TCTTGTGACG TCGTCAGGAA CGTTCCAAAC 
GGTTTACAAG AATTTCTGGA CCCTCTGTGC CAGAGAGGGT TTTGCAGGTT AATTCCTCAT 
GCACGTTCAC CAGGTACTTG GACGATATAT TTTGAAGGTG CAGATTATGA GAGCCATCTT 
CTGCGTGAGA ACACAGAGCT GGCTCAGCCT CTGCGGAAAG AACCTGAAAT AATCACTGTG 
ACTCTCAAAA AGCAAAACGG AATGGGCCTC AGCATTGTCG CAGCAAAGGG TGCTGGTCAA 
GATAAACTTG GAATCTATGT CAAGTCTGTC GTAAAAGGAG GTGCTGCAGA TGTGGATGGA 
CGCCTAGCCG CAGGTGACCA GCTCCTCAGT GTGGATGGAC GAAGTCTGGT AGGACTCTCT 
CAAGAAAGGG CAGCAGAACT CATGACAAGG ACCAGTTCCG TGGTGACACT GGAAGTAGCC 
AAGCAAGGAG CGATCTACCA CGGCCTGGCC ACCCTGCTCA ACCAGCCGTC CCCGATGATG 
CAGAGAGTTT CAGACCGTCG TGGCTCAGGT AAACCCCGAC CAAAGAGCGA AGGTTTCGAG 
CTCTATAACA ATTCAGCTCA AAACGGGTCA CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA 
TACAGCGAAC CGAAGAAGCT CCCTGGCGAT GACAGGCTGA TGAAGAACCG GGCTGACCAC 
CGCTCCAGCC CCAACGTGGC AAATCAGCCC CCCAGTCCCG GAGGGAAAAG CGCGTACGCC 
CCTGGAGCAG CCGCTAAGAT CACGTCCGTC TCCACCGGAA ACCTCTGCAC TGAGGAGCAG 
ACCCCTCCGC CTCGACCTGA GGCCTACCCC ATCCCCACCC AGACATACAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAG GTCCCAGGAC CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC 
AATTACGAGG AGAAGCCGCA CGTGCACACA GATGGTCATC AGTCCGGCCC CGCAATGCAG 
CGCGTCACCC GCTCCCAGGA GGAACTTCGG GAGGATAAAG CTTACCAACT CGAGCGGCAT 
CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC GACAGCGACG TGTGGATCCA GCCGAGCTCC 
TCGGTGGGCT CCAGCACGTC CAGCCAGGAG CACCTGGACC CCTGCTCCAA GTCGGGCACC 
CCCGCCTCCA CGCTGACCAA GAGCGGCCCC GGGCGCTGGA AGACGCCAGC CTCCGCGCAG 
CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG ACAGACCTGC CCCCACCACC CCCGCCGCCC 
CCCGCGCACT ACGCCGGAGA CTTCGACGGG ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT 
CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG AAACGGGAGG AGCAGCAGCG CTGGTACGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GACAGGAAGC GCCGGGAGCA GGAGCGGAAG 
CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC CCGGCCGCGT TCTCCCCAGT GACCGTCGCG 
CACACGAAGC CGGAGAAACC TTCCACGCTC CAGAGGCCCC AGGACACGGT CATCCGGGAG 
CTGCAGCCCC AGCAGCAGCC ACGCACCATC GAGCGCCGAG ACTTGCAGTA CATCACGGTC 
AGCAAAGAGG AGCTGTCCTC GGGGGACAGC CTGTCCCCCG ACCCCTGGAA GCGGGACGCC 
AGGGAGAAGC TGGAGAAGCA GCAGCAAATG CACATCGTGG ACATGCTGAG CAGGGAGATC 
CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC GCCGAGGACA GCGACCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT CGAGGCTGAG 
CGGAGAGCAA GGCAGACTGG TCTGCCAGCA ATCTCTGTTC TAGATTTGTT ACAGGACGAG 
GAGCGGCGGC GGCAGCGGCA GCTGGACGAC ATGCGCAGGC GGGAAGCGGA GGACCGCGCC 
AGGCAGGAGG AGGAGAGGAC GAAGCGAGAC GCCGAAGACA AGAGGCGACA GGAAGAAGGT 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGCTG 
CTGGAGCCGG AGGAGCCGGG CCTGGGCAGA CCGCCGCTGC CGCGGGGCTA CGAGCTCCCG 
GCCCCGTCCT CCCCGGCGGC TGCTCCTCCT CCCCCACCTC AGCGGACCGC CTCCTCCCTG 
CAGGCCCCGG CCCTGTCCCC AGACTCCCTG TACACCGCCA GGCTCGTTGC GTACCACGAG 
GAGGAGGAGG AGGAGGACTG CAGTCTGGCA GGTCAGGACA AGCTCTCCCA CACCGGACCA 
AACTCTTCCG TGGGATCTTC CGGGGCAGCG ATCGGTGCCC GTGACGGCTC CCGGGACCCG 
AGAGAGAGGC TGGCGAAAAG CCATGATGCA GATTTACCTG GAAATTCCGG AGCCCCTGAG 
AACTTGACAT TCAAAGAGCG CCAGCGGCTG TTCTCACAAG GTCAAGATGT GTCCAATAAA 
GTGAAAGCCT CTCGTAAATT AACAGAACTG GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687054.1
CDS	22..5355
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831495.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831495.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGATGGTT CTGACTCCAG GGACAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTCGGGA CAGAGAAGTT CGAGGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGAGTTGTCA CCGTGACTCC GAGAAGCATG GACGCCGAGA CGTACGTGGA AGGACAGCGC 
ATCTCCGAGA CAACCATGCT GCAGAGTGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCAGTGGCCA AGAGACCTGT GGATGGAGGC 
CTGACGGCCA AGGGCCCCAG ACAGAAGCCT GGAATTGTTC AGGAGACAAC TTTCGATTTG 
GGGGGAGATG TTCACAGCGG GGCAGCGCTG CCAGCGAGCA AGAGCGCCAC CAGGCTGGAC 
GGCGACAGGG CACTGCCTGC CCCCAGCACG GCCGAGCGCG GGACAGTGAA GCCGATGCTG 
AGACTGGAGC AGCAGGACTA CCGCCGGCAA GAAAACAGAA CTCAGGATGC TCTTGGGCCT 
GAACTCATAC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC ATTTTTATCT 
GCCATTATAA ATTATACTAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTA 
TTATATATGG CATGTCGGTA TGTATTGTCG AGCCAGTACA GACCCGATGT CAGTCCTACA 
GAGCGTACTC ACAAAGTGAT TGCCATAGTC AACAAGATGG TGAGCATGAT GGAGGGGGTG 
ATTCAGGAAG TAGACCAGGT TGATCAGAAA CAGAAGAATA TCGCAGGGGC ACTTGCTTTC 
TGGATGGCCA ATGCATCTGA GCTTCTCAAC TTCATTAAGC AGGACCGAGA TCTTAGTCGA 
ATCACATTGG ATGCCCAAGA TGTTTTAGCA CACTTGGTTC AAATGGCGTT TAAATATCTG 
GTTCATTGTC TTCAGTCAGA ACTGAATAAC TACATGCCAG CTTTTCTGGA TGACCCTGAA 
GAGAACAGTC TACAAAGACC AAAAATAGAT GACGTCTTGC ACACGCTCAC GGGAGCGATG 
GCGCTGCTGA GGCGCTGCAG GGTCAATGCT GCTCTGACCA TCCAGCTTTT CTCTCAGCTC 
TTCCACTTCA TCAACATGTG GCTCTTCAAC AGATTGGTGA CCGACCCCGA GTCGGGACTG 
TGCTCTCACT ACTGGGGGGC AATCATCCGC CAGCAGCTGG GGCACGTGGA AGCCTGGGCA 
GAGAAGCAGG GATTAGAGCT GGCAGCCGAC TGTCACCTGA GCCGGATAGT GCAGGCGACC 
ACTTTGCTCA CCATGGACAA ATACGCTCCT GAGGACACCC CAAATATCAA CAGCACCTGC 
TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC TTGCTACAGA ATTACCACTG TGCGCCCGAC 
GAGCCCTTTG TCCCCACGGA CCTGATAGAG AATGTCGTGG CCGTGGCTGA AAACACGGCC 
GACGAGCTGG CACGCAGTGA TGGGCGGGAG GTGCAGCTGG AAGAGGACCC TGATCTGCAG 
CTGCCTTTTC TTTTGCCGGA AGACGGCTAT TCTTGTGACG TCGTCAGGAA CGTTCCAAAC 
GGTTTACAAG AATTTCTGGA CCCTCTGTGC CAGAGAGGGT TTTGCAGGTT AATTCCTCAT 
GCACGTTCAC CAGGTACTTG GACGATATAT TTTGAAGGTG CAGATTATGA GAGCCATCTT 
CTGCGTGAGA ACACAGAGCT GGCTCAGCCT CTGCGGAAAG AACCTGAAAT AATCACTGTG 
ACTCTCAAAA AGCAAAACGG AATGGGCCTC AGCATTGTCG CAGCAAAGGG TGCTGGTCAA 
GATAAACTTG GAATCTATGT CAAGTCTGTC GTAAAAGGAG GTGCTGCAGA TGTGGATGGA 
CGCCTAGCCG CAGGTGACCA GCTCCTCAGT GTGGATGGAC GAAGTCTGGT AGGACTCTCT 
CAAGAAAGGG CAGCAGAACT CATGACAAGG ACCAGTTCCG TGGTGACACT GGAAGTAGCC 
AAGCAAGGAG CGATCTACCA CGGCCTGGCC ACCCTGCTCA ACCAGCCGTC CCCGATGATG 
CAGAGAGTTT CAGACCGTCG TGGCTCAGGT AAACCCCGAC CAAAGAGCGA AGGTTTCGAG 
CTCTATAACA ATTCAGCTCA AAACGGGTCA CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA 
TACAGCGAAC CGAAGAAGCT CCCTGGCGAT GACAGGCTGA TGAAGAACCG GGCTGACCAC 
CGCTCCAGCC CCAACGTGGC AAATCAGCCC CCCAGTCCCG GAGGGAAAAG CGCGTACGCC 
CCTGGAGCAG CCGCTAAGAT CACGTCCGTC TCCACCGGAA ACCTCTGCAC TGAGGAGCAG 
ACCCCTCCGC CTCGACCTGA GGCCTACCCC ATCCCCACCC AGACATACAC CAGGGAGTAC 
TTCACCTTCC CGGCCTCCAG GTCCCAGGAC CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC 
AATTACGAGG AGAAGCCGCA CGTGCACACA GATGGTCATC AGTCCGGCCC CGCAATGCAG 
CGCGTCACCC GCTCCCAGGA GGAACTTCGG GAGGATAAAG CTTACCAACT CGAGCGGCAT 
CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC GACAGCGACG TGTGGATCCA GCCGAGCTCC 
TCGGTGGGCT CCAGCACGTC CAGCCAGGAG CACCTGGACC CCTGCTCCAA GTCGGGCACC 
CCCGCCTCCA CGCTGACCAA GAGCGGCCCC GGGCGCTGGA AGACGCCAGC CTCCGCGCAG 
CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG ACAGACCTGC CCCCACCACC CCCGCCGCCC 
CCCGCGCACT ACGCCGGAGA CTTCGACGGG ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT 
CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG AAACGGGAGG AGCAGCAGCG CTGGTACGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GACAGGAAGC GCCGGGAGCA GGAGCGGAAG 
CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC CCGGCCGCGT TCTCCCCAGT GACCGTCGCG 
CACACGAAGC CGGAGAAACC TTCCACGCTC CAGAGGCCCC AGGACACGGT CATCCGGGAG 
CTGCAGCCCC AGCAGCAGCC ACGCACCATC GAGCGCCGAG ACTTGCAGTA CATCACGGTC 
AGCAAAGAGG AGCTGTCCTC GGGGGACAGC CTGTCCCCCG ACCCCTGGAA GCGGGACGCC 
AGGGAGAAGC TGGAGAAGCA GCAGCAAATG CACATCGTGG ACATGCTGAG CAGGGAGATC 
CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC GCCGAGGACA GCGACCGCCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTG CAGGAGTCCA AGCAGAAGGA CGAGGACGAC 
GAGGAGGAGG AGGACGACGA CGTGGACACC ATGCTGATCA TGCAGCGCCT CGAGGCTGAG 
CGGAGAGCAA GGCAGACTGG TCTGCCAGCA ATCTCTGTTC TAGATTTGTT ACAGGACGAG 
GAGCGGCGGC GGCAGCGGCA GCTGGACGAC ATGCGCAGGC GGGAAGCGGA GGACCGCGCC 
AGGCAGGAGG AGGAGAGGAC GAAGCGAGAC GCCGAAGACA AGAGGCGACA GGAAGAAGGT 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGCTG 
CTGGAGCCGG AGGAGCCGGG CCTGGGCAGA CCGCCGCTGC CGCGGGGCTA CGAGCTCCCG 
GCCCCGTCCT CCCCGGCGGC TGCTCCTCCT CCCCCACCTC AGCGGACCGC CTCCTCCCTG 
CAGGCCCCGG CCCTGTCCCC AGACTCCCTG TACACCGCCA GGCTCGTTGC GTACCACGAG 
GAGGAGGAGG AGGAGGACTG CAGTCTGGCA GGTCAGGACA AGCTCTCCCA CACCGGACCA 
AACTCTTCCG TGGGATCTTC CGGGGCAGCG ATCGGTGCCC GTGACGGCTC CCGGGACCCG 
AGAGAGAGGC TGGCGAAAAG CCATGATGCA GATTTACCTG GAAATTCCGG AGCCCCTGAG 
AACTTGACAT TCAAAGAGCG CCAGCGGCTG TTCTCACAAG GTCAAGATGT GTCCAATAAA 
GTGAAAGCCT CTCGTAAATT AACAGAACTG GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831496.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687055.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

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CloneID	OFb43232
Clone ID Related Accession (Same CDS sequence)	XM_019831496.1
Accession Version	XM_019831496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGTTACAGG ACGAGGAGCG GCGGCGGCAG CGGCAGCTGG ACGACATGCG CAGGCGGGAA 
GCGGAGGACC GCGCCAGGCA GGAGGAGGAG AGGACGAAGC GAGACGCCGA AGACAAGAGG 
CGACAGGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG CCGGGCCTGG GCAGACCGCC GCTGCCGCGG 
GGCTACGAGC TCCCGGCCCC GTCCTCCCCG GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG 
ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG TCCCCAGACT CCCTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGA GGAGGAGGAG GACTGCAGTC TGGCAGGACC AAACTCTTCC 
GTGGGATCTT CCGGGGCAGC GATCGGTGCC CGTGACGGCT CCCGGGACCC GAGAGAGAGG 
CTGGCGAAAA GCCATGATGC AGATTTACCT GGAAATTCCG GAGCCCCTGA GAACTTGACA 
TTCAAAGAGC GCCAGCGGCT GTTCTCACAA GGTCAAGATG TGTCCAATAA AGTGAAAGCC 
TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687055.1
CDS	22..5346
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831496.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831496.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGTTACAGG ACGAGGAGCG GCGGCGGCAG CGGCAGCTGG ACGACATGCG CAGGCGGGAA 
GCGGAGGACC GCGCCAGGCA GGAGGAGGAG AGGACGAAGC GAGACGCCGA AGACAAGAGG 
CGACAGGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG CCGGGCCTGG GCAGACCGCC GCTGCCGCGG 
GGCTACGAGC TCCCGGCCCC GTCCTCCCCG GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG 
ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG TCCCCAGACT CCCTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGA GGAGGAGGAG GACTGCAGTC TGGCAGGACC AAACTCTTCC 
GTGGGATCTT CCGGGGCAGC GATCGGTGCC CGTGACGGCT CCCGGGACCC GAGAGAGAGG 
CTGGCGAAAA GCCATGATGC AGATTTACCT GGAAATTCCG GAGCCCCTGA GAACTTGACA 
TTCAAAGAGC GCCAGCGGCT GTTCTCACAA GGTCAAGATG TGTCCAATAA AGTGAAAGCC 
TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831497.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687056.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OFb43233
Clone ID Related Accession (Same CDS sequence)	XM_019831497.1
Accession Version	XM_019831497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAGAAGA AGGAGAAAAA GAAGAGAGAG AAAGAGGCAG TGAGGCAGGC ATCTGAGAAA 
GATGATCGGC CTTTCCAAGG GGATGACCTC GAGAATTCCC GACTGGCCGC CGAGGTTTAC 
AAAGACATGC CCGAGACCAG CTTCACTCGC ACGATCTCCA ACCCCGAGGT GGTCATGAAG 
CGCCGGCGGC AGCAGAAGCT GGAGAGGCGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCGGACTCAG GTGGAACACT GAGAATTTAT GCAGATAGCT TAAAACCGAA CATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGATCCT GCTGACTTCG CTGTGGCCGA GGCTCTAGAG 
AAATACGGTC TGGAGAAGGA GAACCCCAAA GATTACTGCA TCGCCCGGGT TATGCTGCCT 
CCTGGAGCCC AGCATTCGGA TGAAAACGGT GCTAAAGAAA CGATCCTCGA TGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAGTGGCCA ACTGACCGAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGGC CGCCAGACTA CGTCCCGAAG AAAACGAAGA AACATGTCGA CGGGAAGCTG 
GTGAAAGGGA AGGAGAGAGC CGAGGGTTCC GGCTATGGCT CAGCACTTCC GCCTGAGAAG 
TTGCCCTACC TGGTGGAGCT CAGCCCAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC TGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACATA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCAATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGCG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCACC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAGAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGCAGAC TGGTCTGCCA 
GCAATCTCTG TTCTAGATTT GTTACAGGAC GAGGAGCGGC GGCGGCAGCG GCAGCTGGAC 
GACATGCGCA GGCGGGAAGC GGAGGACCGC GCCAGGCAGG AGGAGGAGAG GACGAAGCGA 
GACGCCGAAG ACAAGAGGCG ACAGGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG CTGCTGGAGC CGGAGGAGCC GGGCCTGGGC 
AGACCGCCGC TGCCGCGGGG CTACGAGCTC CCGGCCCCGT CCTCCCCGGC GGCTGCTCCT 
CCTCCCCCAC CTCAGCGGAC CGCCTCCTCC CTGCAGGCCC CGGCCCTGTC CCCAGACTCC 
CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGGA CTGCAGTCTG 
GCAGGTCAGG ACAAGCTCTC CCACACCGGA CCAAACTCTT CCGTGGGATC TTCCGGGGCA 
GCGATCGGTG CCCGTGACGG CTCCCGGGAC CCGAGAGAGA GGCTGGCGAA AAGCCATGAT 
GCAGATTTAC CTGGAAATTC CGGAGCCCCT GAGAACTTGA CATTCAAAGA GCGCCAGCGG 
CTGTTCTCAC AAGGTCAAGA TGTGTCCAAT AAAGTGAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687056.1
CDS	22..5328
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831497.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831497.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAGAAGA AGGAGAAAAA GAAGAGAGAG AAAGAGGCAG TGAGGCAGGC ATCTGAGAAA 
GATGATCGGC CTTTCCAAGG GGATGACCTC GAGAATTCCC GACTGGCCGC CGAGGTTTAC 
AAAGACATGC CCGAGACCAG CTTCACTCGC ACGATCTCCA ACCCCGAGGT GGTCATGAAG 
CGCCGGCGGC AGCAGAAGCT GGAGAGGCGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCGGACTCAG GTGGAACACT GAGAATTTAT GCAGATAGCT TAAAACCGAA CATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGATCCT GCTGACTTCG CTGTGGCCGA GGCTCTAGAG 
AAATACGGTC TGGAGAAGGA GAACCCCAAA GATTACTGCA TCGCCCGGGT TATGCTGCCT 
CCTGGAGCCC AGCATTCGGA TGAAAACGGT GCTAAAGAAA CGATCCTCGA TGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAGTGGCCA ACTGACCGAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGGC CGCCAGACTA CGTCCCGAAG AAAACGAAGA AACATGTCGA CGGGAAGCTG 
GTGAAAGGGA AGGAGAGAGC CGAGGGTTCC GGCTATGGCT CAGCACTTCC GCCTGAGAAG 
TTGCCCTACC TGGTGGAGCT CAGCCCAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC TGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACATA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCAATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGCG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCACC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAGAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGCAGAC TGGTCTGCCA 
GCAATCTCTG TTCTAGATTT GTTACAGGAC GAGGAGCGGC GGCGGCAGCG GCAGCTGGAC 
GACATGCGCA GGCGGGAAGC GGAGGACCGC GCCAGGCAGG AGGAGGAGAG GACGAAGCGA 
GACGCCGAAG ACAAGAGGCG ACAGGAAGAA GGTTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGCGGAGGC CGAGCGCAGG CTGCTGGAGC CGGAGGAGCC GGGCCTGGGC 
AGACCGCCGC TGCCGCGGGG CTACGAGCTC CCGGCCCCGT CCTCCCCGGC GGCTGCTCCT 
CCTCCCCCAC CTCAGCGGAC CGCCTCCTCC CTGCAGGCCC CGGCCCTGTC CCCAGACTCC 
CTGTACACCG CCAGGCTCGT TGCGTACCAC GAGGAGGAGG AGGAGGAGGA CTGCAGTCTG 
GCAGGTCAGG ACAAGCTCTC CCACACCGGA CCAAACTCTT CCGTGGGATC TTCCGGGGCA 
GCGATCGGTG CCCGTGACGG CTCCCGGGAC CCGAGAGAGA GGCTGGCGAA AAGCCATGAT 
GCAGATTTAC CTGGAAATTC CGGAGCCCCT GAGAACTTGA CATTCAAAGA GCGCCAGCGG 
CTGTTCTCAC AAGGTCAAGA TGTGTCCAAT AAAGTGAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831498.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687057.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OFb43234
Clone ID Related Accession (Same CDS sequence)	XM_019831498.1
Accession Version	XM_019831498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGAAA 
CAGAAGAATA TCGCAGGGGC ACTTGCTTTC TGGATGGCCA ATGCATCTGA GCTTCTCAAC 
TTCATTAAGC AGGACCGAGA TCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTTTAGCA 
CACTTGGTTC AAATGGCGTT TAAATATCTG GTTCATTGTC TTCAGTCAGA ACTGAATAAC 
TACATGCCAG CTTTTCTGGA TGACCCTGAA GAGAACAGTC TACAAAGACC AAAAATAGAT 
GACGTCTTGC ACACGCTCAC GGGAGCGATG GCGCTGCTGA GGCGCTGCAG GGTCAATGCT 
GCTCTGACCA TCCAGCTTTT CTCTCAGCTC TTCCACTTCA TCAACATGTG GCTCTTCAAC 
AGATTGGTGA CCGACCCCGA GTCGGGACTG TGCTCTCACT ACTGGGGGGC AATCATCCGC 
CAGCAGCTGG GGCACGTGGA AGCCTGGGCA GAGAAGCAGG GATTAGAGCT GGCAGCCGAC 
TGTCACCTGA GCCGGATAGT GCAGGCGACC ACTTTGCTCA CCATGGACAA ATACGCTCCT 
GAGGACACCC CAAATATCAA CAGCACCTGC TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC 
TTGCTACAGA ATTACCACTG TGCGCCCGAC GAGCCCTTTG TCCCCACGGA CCTGATAGAG 
AATGTCGTGG CCGTGGCTGA AAACACGGCC GACGAGCTGG CACGCAGTGA TGGGCGGGAG 
GTGCAGCTGG AAGAGGACCC TGATCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGCTAT 
TCTTGTGACG TCGTCAGGAA CGTTCCAAAC GGTTTACAAG AATTTCTGGA CCCTCTGTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAT GCACGTTCAC CAGGTACTTG GACGATATAT 
TTTGAAGGTG CAGATTATGA GAGCCATCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGCGGAAAG AACCTGAAAT AATCACTGTG ACTCTCAAAA AGCAAAACGG AATGGGCCTC 
AGCATTGTCG CAGCAAAGGG TGCTGGTCAA GATAAACTTG GAATCTATGT CAAGTCTGTC 
GTAAAAGGAG GTGCTGCAGA TGTGGATGGA CGCCTAGCCG CAGGTGACCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT AGGACTCTCT CAAGAAAGGG CAGCAGAACT CATGACAAGG 
ACCAGTTCCG TGGTGACACT GGAAGTAGCC AAGCAAGGAG CGATCTACCA CGGCCTGGCC 
ACCCTGCTCA ACCAGCCGTC CCCGATGATG CAGAGAGTTT CAGACCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTCGAG CTCTATAACA ATTCAGCTCA AAACGGGTCA 
CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA TACAGCGAAC CGAAGAAGCT CCCTGGCGAT 
GACAGGCTGA TGAAGAACCG GGCTGACCAC CGCTCCAGCC CCAACGTGGC AAATCAGCCC 
CCCAGTCCCG GAGGGAAAAG CGCGTACGCC CCTGGAGCAG CCGCTAAGAT CACGTCCGTC 
TCCACCGGAA ACCTCTGCAC TGAGGAGCAG ACCCCTCCGC CTCGACCTGA GGCCTACCCC 
ATCCCCACCC AGACATACAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC AATTACGAGG AGAAGCCGCA CGTGCACACA 
GATGGTCATC AGTCCGGCCC CGCAATGCAG CGCGTCACCC GCTCCCAGGA GGAACTTCGG 
GAGGATAAAG CTTACCAACT CGAGCGGCAT CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC 
GACAGCGACG TGTGGATCCA GCCGAGCTCC TCGGTGGGCT CCAGCACGTC CAGCCAGGAG 
CACCTGGACC CCTGCTCCAA GTCGGGCACC CCCGCCTCCA CGCTGACCAA GAGCGGCCCC 
GGGCGCTGGA AGACGCCAGC CTCCGCGCAG CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG 
ACAGACCTGC CCCCACCACC CCCGCCGCCC CCCGCGCACT ACGCCGGAGA CTTCGACGGG 
ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG 
AAACGGGAGG AGCAGCAGCG CTGGTACGAG AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG 
GACAGGAAGC GCCGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC 
CCGGCCGCGT TCTCCCCAGT GACCGTCGCG CACACGAAGC CGGAGAAACC TTCCACGCTC 
CAGAGGCCCC AGGACACGGT CATCCGGGAG CTGCAGCCCC AGCAGCAGCC ACGCACCATC 
GAGCGCCGAG ACTTGCAGTA CATCACGGTC AGCAAAGAGG AGCTGTCCTC GGGGGACAGC 
CTGTCCCCCG ACCCCTGGAA GCGGGACGCC AGGGAGAAGC TGGAGAAGCA GCAGCAAATG 
CACATCGTGG ACATGCTGAG CAGGGAGATC CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC 
GCCGAGGACA GCGACCGCCT GCGCAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTG 
CAGGAGTCCA AGCAGAAGGA CGAGGACGAC GAGGAGGAGG AGGACGACGA CGTGGACACC 
ATGCTGATCA TGCAGCGCCT CGAGGCTGAG CGGAGAGCAA GGTTACAGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGACCA AACTCTTCCG TGGGATCTTC CGGGGCAGCG 
ATCGGTGCCC GTGACGGCTC CCGGGACCCG AGAGAGAGGC TGGCGAAAAG CCATGATGCA 
GATTTACCTG GAAATTCCGG AGCCCCTGAG AACTTGACAT TCAAAGAGCG CCAGCGGCTG 
TTCTCACAAG GTCAAGATGT GTCCAATAAA GTGAAAGCCT CTCGTAAATT AACAGAACTG 
GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687057.1
CDS	22..5325
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831498.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831498.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGAAA 
CAGAAGAATA TCGCAGGGGC ACTTGCTTTC TGGATGGCCA ATGCATCTGA GCTTCTCAAC 
TTCATTAAGC AGGACCGAGA TCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTTTAGCA 
CACTTGGTTC AAATGGCGTT TAAATATCTG GTTCATTGTC TTCAGTCAGA ACTGAATAAC 
TACATGCCAG CTTTTCTGGA TGACCCTGAA GAGAACAGTC TACAAAGACC AAAAATAGAT 
GACGTCTTGC ACACGCTCAC GGGAGCGATG GCGCTGCTGA GGCGCTGCAG GGTCAATGCT 
GCTCTGACCA TCCAGCTTTT CTCTCAGCTC TTCCACTTCA TCAACATGTG GCTCTTCAAC 
AGATTGGTGA CCGACCCCGA GTCGGGACTG TGCTCTCACT ACTGGGGGGC AATCATCCGC 
CAGCAGCTGG GGCACGTGGA AGCCTGGGCA GAGAAGCAGG GATTAGAGCT GGCAGCCGAC 
TGTCACCTGA GCCGGATAGT GCAGGCGACC ACTTTGCTCA CCATGGACAA ATACGCTCCT 
GAGGACACCC CAAATATCAA CAGCACCTGC TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC 
TTGCTACAGA ATTACCACTG TGCGCCCGAC GAGCCCTTTG TCCCCACGGA CCTGATAGAG 
AATGTCGTGG CCGTGGCTGA AAACACGGCC GACGAGCTGG CACGCAGTGA TGGGCGGGAG 
GTGCAGCTGG AAGAGGACCC TGATCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGCTAT 
TCTTGTGACG TCGTCAGGAA CGTTCCAAAC GGTTTACAAG AATTTCTGGA CCCTCTGTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAT GCACGTTCAC CAGGTACTTG GACGATATAT 
TTTGAAGGTG CAGATTATGA GAGCCATCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGCGGAAAG AACCTGAAAT AATCACTGTG ACTCTCAAAA AGCAAAACGG AATGGGCCTC 
AGCATTGTCG CAGCAAAGGG TGCTGGTCAA GATAAACTTG GAATCTATGT CAAGTCTGTC 
GTAAAAGGAG GTGCTGCAGA TGTGGATGGA CGCCTAGCCG CAGGTGACCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT AGGACTCTCT CAAGAAAGGG CAGCAGAACT CATGACAAGG 
ACCAGTTCCG TGGTGACACT GGAAGTAGCC AAGCAAGGAG CGATCTACCA CGGCCTGGCC 
ACCCTGCTCA ACCAGCCGTC CCCGATGATG CAGAGAGTTT CAGACCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTCGAG CTCTATAACA ATTCAGCTCA AAACGGGTCA 
CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA TACAGCGAAC CGAAGAAGCT CCCTGGCGAT 
GACAGGCTGA TGAAGAACCG GGCTGACCAC CGCTCCAGCC CCAACGTGGC AAATCAGCCC 
CCCAGTCCCG GAGGGAAAAG CGCGTACGCC CCTGGAGCAG CCGCTAAGAT CACGTCCGTC 
TCCACCGGAA ACCTCTGCAC TGAGGAGCAG ACCCCTCCGC CTCGACCTGA GGCCTACCCC 
ATCCCCACCC AGACATACAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC AATTACGAGG AGAAGCCGCA CGTGCACACA 
GATGGTCATC AGTCCGGCCC CGCAATGCAG CGCGTCACCC GCTCCCAGGA GGAACTTCGG 
GAGGATAAAG CTTACCAACT CGAGCGGCAT CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC 
GACAGCGACG TGTGGATCCA GCCGAGCTCC TCGGTGGGCT CCAGCACGTC CAGCCAGGAG 
CACCTGGACC CCTGCTCCAA GTCGGGCACC CCCGCCTCCA CGCTGACCAA GAGCGGCCCC 
GGGCGCTGGA AGACGCCAGC CTCCGCGCAG CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG 
ACAGACCTGC CCCCACCACC CCCGCCGCCC CCCGCGCACT ACGCCGGAGA CTTCGACGGG 
ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG 
AAACGGGAGG AGCAGCAGCG CTGGTACGAG AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG 
GACAGGAAGC GCCGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC 
CCGGCCGCGT TCTCCCCAGT GACCGTCGCG CACACGAAGC CGGAGAAACC TTCCACGCTC 
CAGAGGCCCC AGGACACGGT CATCCGGGAG CTGCAGCCCC AGCAGCAGCC ACGCACCATC 
GAGCGCCGAG ACTTGCAGTA CATCACGGTC AGCAAAGAGG AGCTGTCCTC GGGGGACAGC 
CTGTCCCCCG ACCCCTGGAA GCGGGACGCC AGGGAGAAGC TGGAGAAGCA GCAGCAAATG 
CACATCGTGG ACATGCTGAG CAGGGAGATC CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC 
GCCGAGGACA GCGACCGCCT GCGCAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTG 
CAGGAGTCCA AGCAGAAGGA CGAGGACGAC GAGGAGGAGG AGGACGACGA CGTGGACACC 
ATGCTGATCA TGCAGCGCCT CGAGGCTGAG CGGAGAGCAA GGTTACAGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGACCA AACTCTTCCG TGGGATCTTC CGGGGCAGCG 
ATCGGTGCCC GTGACGGCTC CCGGGACCCG AGAGAGAGGC TGGCGAAAAG CCATGATGCA 
GATTTACCTG GAAATTCCGG AGCCCCTGAG AACTTGACAT TCAAAGAGCG CCAGCGGCTG 
TTCTCACAAG GTCAAGATGT GTCCAATAAA GTGAAAGCCT CTCGTAAATT AACAGAACTG 
GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831499.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687058.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OFb43235
Clone ID Related Accession (Same CDS sequence)	XM_019831499.1
Accession Version	XM_019831499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGAAA 
CAGAAGAATA TCGCAGGGGC ACTTGCTTTC TGGATGGCCA ATGCATCTGA GCTTCTCAAC 
TTCATTAAGC AGGACCGAGA TCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTTTAGCA 
CACTTGGTTC AAATGGCGTT TAAATATCTG GTTCATTGTC TTCAGTCAGA ACTGAATAAC 
TACATGCCAG CTTTTCTGGA TGACCCTGAA GAGAACAGTC TACAAAGACC AAAAATAGAT 
GACGTCTTGC ACACGCTCAC GGGAGCGATG GCGCTGCTGA GGCGCTGCAG GGTCAATGCT 
GCTCTGACCA TCCAGCTTTT CTCTCAGCTC TTCCACTTCA TCAACATGTG GCTCTTCAAC 
AGATTGGTGA CCGACCCCGA GTCGGGACTG TGCTCTCACT ACTGGGGGGC AATCATCCGC 
CAGCAGCTGG GGCACGTGGA AGCCTGGGCA GAGAAGCAGG GATTAGAGCT GGCAGCCGAC 
TGTCACCTGA GCCGGATAGT GCAGGCGACC ACTTTGCTCA CCATGGACAA ATACGCTCCT 
GAGGACACCC CAAATATCAA CAGCACCTGC TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC 
TTGCTACAGA ATTACCACTG TGCGCCCGAC GAGCCCTTTG TCCCCACGGA CCTGATAGAG 
AATGTCGTGG CCGTGGCTGA AAACACGGCC GACGAGCTGG CACGCAGTGA TGGGCGGGAG 
GTGCAGCTGG AAGAGGACCC TGATCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGCTAT 
TCTTGTGACG TCGTCAGGAA CGTTCCAAAC GGTTTACAAG AATTTCTGGA CCCTCTGTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAT GCACGTTCAC CAGGTACTTG GACGATATAT 
TTTGAAGGTG CAGATTATGA GAGCCATCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGCGGAAAG AACCTGAAAT AATCACTGTG ACTCTCAAAA AGCAAAACGG AATGGGCCTC 
AGCATTGTCG CAGCAAAGGG TGCTGGTCAA GATAAACTTG GAATCTATGT CAAGTCTGTC 
GTAAAAGGAG GTGCTGCAGA TGTGGATGGA CGCCTAGCCG CAGGTGACCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT AGGACTCTCT CAAGAAAGGG CAGCAGAACT CATGACAAGG 
ACCAGTTCCG TGGTGACACT GGAAGTAGCC AAGCAAGGAG CGATCTACCA CGGCCTGGCC 
ACCCTGCTCA ACCAGCCGTC CCCGATGATG CAGAGAGTTT CAGACCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTCGAG CTCTATAACA ATTCAGCTCA AAACGGGTCA 
CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA TACAGCGAAC CGAAGAAGCT CCCTGGCGAT 
GACAGGCTGA TGAAGAACCG GGCTGACCAC CGCTCCAGCC CCAACGTGGC AAATCAGCCC 
CCCAGTCCCG GAGGGAAAAG CGCGTACGCC CCTGGAGCAG CCGCTAAGAT CACGTCCGTC 
TCCACCGGAA ACCTCTGCAC TGAGGAGCAG ACCCCTCCGC CTCGACCTGA GGCCTACCCC 
ATCCCCACCC AGACATACAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC AATTACGAGG AGAAGCCGCA CGTGCACACA 
GATGGTCATC AGTCCGGCCC CGCAATGCAG CGCGTCACCC GCTCCCAGGA GGAACTTCGG 
GAGGATAAAG CTTACCAACT CGAGCGGCAT CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC 
GACAGCGACG TGTGGATCCA GCCGAGCTCC TCGGTGGGCT CCAGCACGTC CAGCCAGGAG 
CACCTGGACC CCTGCTCCAA GTCGGGCACC CCCGCCTCCA CGCTGACCAA GAGCGGCCCC 
GGGCGCTGGA AGACGCCAGC CTCCGCGCAG CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG 
ACAGACCTGC CCCCACCACC CCCGCCGCCC CCCGCGCACT ACGCCGGAGA CTTCGACGGG 
ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG 
AAACGGGAGG AGCAGCAGCG CTGGTACGAG AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG 
GACAGGAAGC GCCGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC 
CCGGCCGCGT TCTCCCCAGT GACCGTCGCG CACACGAAGC CGGAGAAACC TTCCACGCTC 
CAGAGGCCCC AGGACACGGT CATCCGGGAG CTGCAGCCCC AGCAGCAGCC ACGCACCATC 
GAGCGCCGAG ACTTGCAGTA CATCACGGTC AGCAAAGAGG AGCTGTCCTC GGGGGACAGC 
CTGTCCCCCG ACCCCTGGAA GCGGGACGCC AGGGAGAAGC TGGAGAAGCA GCAGCAAATG 
CACATCGTGG ACATGCTGAG CAGGGAGATC CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC 
GCCGAGGACA GCGACCGCCT GCGCAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTG 
CAGGAGTCCA AGCAGAAGGA CGAGGACGAC GAGGAGGAGG AGGACGACGA CGTGGACACC 
ATGCTGATCA TGCAGCGCCT CGAGGCTGAG CGGAGAGCAA GGGACGAGGA GCGGCGGCGG 
CAGCGGCAGC TGGACGACAT GCGCAGGCGG GAAGCGGAGG ACCGCGCCAG GCAGGAGGAG 
GAGAGGACGA AGCGAGACGC CGAAGACAAG AGGCGACAGG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGCTGCT GGAGCCGGAG 
GAGCCGGGCC TGGGCAGACC GCCGCTGCCG CGGGGCTACG AGCTCCCGGC CCCGTCCTCC 
CCGGCGGCTG CTCCTCCTCC CCCACCTCAG CGGACCGCCT CCTCCCTGCA GGCCCCGGCC 
CTGTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG 
GAGGACTGCA GTCTGGCAGG ACCAAACTCT TCCGTGGGAT CTTCCGGGGC AGCGATCGGT 
GCCCGTGACG GCTCCCGGGA CCCGAGAGAG AGGCTGGCGA AAAGCCATGA TGCAGATTTA 
CCTGGAAATT CCGGAGCCCC TGAGAACTTG ACATTCAAAG AGCGCCAGCG GCTGTTCTCA 
CAAGGTCAAG ATGTGTCCAA TAAAGTGAAA GCCTCTCGTA AATTAACAGA ACTGGAAAAC 
GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687058.1
CDS	22..5319
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831499.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831499.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGAAA 
CAGAAGAATA TCGCAGGGGC ACTTGCTTTC TGGATGGCCA ATGCATCTGA GCTTCTCAAC 
TTCATTAAGC AGGACCGAGA TCTTAGTCGA ATCACATTGG ATGCCCAAGA TGTTTTAGCA 
CACTTGGTTC AAATGGCGTT TAAATATCTG GTTCATTGTC TTCAGTCAGA ACTGAATAAC 
TACATGCCAG CTTTTCTGGA TGACCCTGAA GAGAACAGTC TACAAAGACC AAAAATAGAT 
GACGTCTTGC ACACGCTCAC GGGAGCGATG GCGCTGCTGA GGCGCTGCAG GGTCAATGCT 
GCTCTGACCA TCCAGCTTTT CTCTCAGCTC TTCCACTTCA TCAACATGTG GCTCTTCAAC 
AGATTGGTGA CCGACCCCGA GTCGGGACTG TGCTCTCACT ACTGGGGGGC AATCATCCGC 
CAGCAGCTGG GGCACGTGGA AGCCTGGGCA GAGAAGCAGG GATTAGAGCT GGCAGCCGAC 
TGTCACCTGA GCCGGATAGT GCAGGCGACC ACTTTGCTCA CCATGGACAA ATACGCTCCT 
GAGGACACCC CAAATATCAA CAGCACCTGC TTTAAGTTAA ATTCGCTGCA GCTGCAGGCC 
TTGCTACAGA ATTACCACTG TGCGCCCGAC GAGCCCTTTG TCCCCACGGA CCTGATAGAG 
AATGTCGTGG CCGTGGCTGA AAACACGGCC GACGAGCTGG CACGCAGTGA TGGGCGGGAG 
GTGCAGCTGG AAGAGGACCC TGATCTGCAG CTGCCTTTTC TTTTGCCGGA AGACGGCTAT 
TCTTGTGACG TCGTCAGGAA CGTTCCAAAC GGTTTACAAG AATTTCTGGA CCCTCTGTGC 
CAGAGAGGGT TTTGCAGGTT AATTCCTCAT GCACGTTCAC CAGGTACTTG GACGATATAT 
TTTGAAGGTG CAGATTATGA GAGCCATCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGCGGAAAG AACCTGAAAT AATCACTGTG ACTCTCAAAA AGCAAAACGG AATGGGCCTC 
AGCATTGTCG CAGCAAAGGG TGCTGGTCAA GATAAACTTG GAATCTATGT CAAGTCTGTC 
GTAAAAGGAG GTGCTGCAGA TGTGGATGGA CGCCTAGCCG CAGGTGACCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT AGGACTCTCT CAAGAAAGGG CAGCAGAACT CATGACAAGG 
ACCAGTTCCG TGGTGACACT GGAAGTAGCC AAGCAAGGAG CGATCTACCA CGGCCTGGCC 
ACCCTGCTCA ACCAGCCGTC CCCGATGATG CAGAGAGTTT CAGACCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGCGA AGGTTTCGAG CTCTATAACA ATTCAGCTCA AAACGGGTCA 
CCCGAGAGCC CTCAGCTGCC TTGGGCAGAA TACAGCGAAC CGAAGAAGCT CCCTGGCGAT 
GACAGGCTGA TGAAGAACCG GGCTGACCAC CGCTCCAGCC CCAACGTGGC AAATCAGCCC 
CCCAGTCCCG GAGGGAAAAG CGCGTACGCC CCTGGAGCAG CCGCTAAGAT CACGTCCGTC 
TCCACCGGAA ACCTCTGCAC TGAGGAGCAG ACCCCTCCGC CTCGACCTGA GGCCTACCCC 
ATCCCCACCC AGACATACAC CAGGGAGTAC TTCACCTTCC CGGCCTCCAG GTCCCAGGAC 
CGCGTGGCTC CCCCTCAGAA CCAGTGGCCC AATTACGAGG AGAAGCCGCA CGTGCACACA 
GATGGTCATC AGTCCGGCCC CGCAATGCAG CGCGTCACCC GCTCCCAGGA GGAACTTCGG 
GAGGATAAAG CTTACCAACT CGAGCGGCAT CGGCTGGAGG CGGTGCTGGA TCGCAAGTCC 
GACAGCGACG TGTGGATCCA GCCGAGCTCC TCGGTGGGCT CCAGCACGTC CAGCCAGGAG 
CACCTGGACC CCTGCTCCAA GTCGGGCACC CCCGCCTCCA CGCTGACCAA GAGCGGCCCC 
GGGCGCTGGA AGACGCCAGC CTCCGCGCAG CCCCTGCCGG CGGCCCCCCC GCCCGCGCAG 
ACAGACCTGC CCCCACCACC CCCGCCGCCC CCCGCGCACT ACGCCGGAGA CTTCGACGGG 
ATCCCCGTGG ACCTGCCCCT CCCGCCCCCT CCCAGCGGCC AGGCGGCGGC GGAGCGGAGG 
AAACGGGAGG AGCAGCAGCG CTGGTACGAG AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG 
GACAGGAAGC GCCGGGAGCA GGAGCGGAAG CTGGGCCAGA TGCGCTCCCA GGCCCTGAAC 
CCGGCCGCGT TCTCCCCAGT GACCGTCGCG CACACGAAGC CGGAGAAACC TTCCACGCTC 
CAGAGGCCCC AGGACACGGT CATCCGGGAG CTGCAGCCCC AGCAGCAGCC ACGCACCATC 
GAGCGCCGAG ACTTGCAGTA CATCACGGTC AGCAAAGAGG AGCTGTCCTC GGGGGACAGC 
CTGTCCCCCG ACCCCTGGAA GCGGGACGCC AGGGAGAAGC TGGAGAAGCA GCAGCAAATG 
CACATCGTGG ACATGCTGAG CAGGGAGATC CGGGAGCTCC AGAGCAAGCC CGAGCGCAGC 
GCCGAGGACA GCGACCGCCT GCGCAAGCTC ATGCTGGAGT GGCAGTTCCA GAAGAGACTG 
CAGGAGTCCA AGCAGAAGGA CGAGGACGAC GAGGAGGAGG AGGACGACGA CGTGGACACC 
ATGCTGATCA TGCAGCGCCT CGAGGCTGAG CGGAGAGCAA GGGACGAGGA GCGGCGGCGG 
CAGCGGCAGC TGGACGACAT GCGCAGGCGG GAAGCGGAGG ACCGCGCCAG GCAGGAGGAG 
GAGAGGACGA AGCGAGACGC CGAAGACAAG AGGCGACAGG AAGAAGGTTA TTACAGCCGC 
CTGGAAGCCG AGAGGCGCAG ACAGCACGCG GAGGCCGAGC GCAGGCTGCT GGAGCCGGAG 
GAGCCGGGCC TGGGCAGACC GCCGCTGCCG CGGGGCTACG AGCTCCCGGC CCCGTCCTCC 
CCGGCGGCTG CTCCTCCTCC CCCACCTCAG CGGACCGCCT CCTCCCTGCA GGCCCCGGCC 
CTGTCCCCAG ACTCCCTGTA CACCGCCAGG CTCGTTGCGT ACCACGAGGA GGAGGAGGAG 
GAGGACTGCA GTCTGGCAGG ACCAAACTCT TCCGTGGGAT CTTCCGGGGC AGCGATCGGT 
GCCCGTGACG GCTCCCGGGA CCCGAGAGAG AGGCTGGCGA AAAGCCATGA TGCAGATTTA 
CCTGGAAATT CCGGAGCCCC TGAGAACTTG ACATTCAAAG AGCGCCAGCG GCTGTTCTCA 
CAAGGTCAAG ATGTGTCCAA TAAAGTGAAA GCCTCTCGTA AATTAACAGA ACTGGAAAAC 
GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831500.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687059.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OFb43236
Clone ID Related Accession (Same CDS sequence)	XM_019831500.1
Accession Version	XM_019831500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGCAGACTG GTCTGCCAGC AATCTCTGTT CTAGATTTGT TACAGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGTCAGGAC AAGCTCTCCC ACACCGGACC AAACTCTTCC 
GTGGGATCTT CCGGGGCAGC GATCGGTGCC CGTGACGGCT CCCGGGACCC GAGAGAGAGG 
CTGGCGAAAA GCCATGATGC AGATTTACCT GGAAATTCCG GAGCCCCTGA GAACTTGACA 
TTCAAAGAGC GCCAGCGGCT GTTCTCACAA GGTCAAGATG TGTCCAATAA AGTGAAAGCC 
TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687059.1
CDS	74..5458
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831500.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831500.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAGAA 
AGAAGATTGG ATGTAGATGA GAAACCCCTT GTTGTGCAGT TGAATTGGAA TAAAGACGAT 
CGAGAGGGCA GATTTGTTCT CAAGAATGAG AACGATGCCC TTCCTCCTAA GAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTCGCTGT GGCCGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGTCCCAG GGAGAAGGAG TCACTTTGCC 
TACTACAGCT ATCACACTTA CGAAGATGGT TCTGACTCCA GGGACAAGCC CAAGCTTTAC 
CGCCTTCAGT TGAGCGTTAC TGAGGTCGGG ACAGAGAAGT TCGAGGAGAA CTCCATCCAG 
CTGTTTGGAT CAGGAATTCA GCCCCATCAC TGTGACCTCA CAAACATGGA TGGAGTTGTC 
ACCGTGACTC CGAGAAGCAT GGACGCCGAG ACGTACGTGG AAGGACAGCG CATCTCCGAG 
ACAACCATGC TGCAGAGTGG GATGAAAGTG CAGTTTGGGG CGTCACACGT GTTCAAGTTT 
GTGGACCCCA GCCAGGACCA CGCAGTGGCC AAGAGACCTG TGGATGGAGG CCTGACGGCC 
AAGGGCCCCA GACAGAAGCC TGGAATTGTT CAGGAGACAA CTTTCGATTT GGGGGGAGAT 
GTTCACAGCG GGGCAGCGCT GCCAGCGAGC AAGAGCGCCA CCAGGCTGGA CGGCGACAGG 
GCACTGCCTG CCCCCAGCAC GGCCGAGCGC GGGACAGTGA AGCCGATGCT GAGACTGGAG 
CAGCAGGACT ACCGCCGGCA AGAAAACAGA ACTCAGGATG CTCTTGGGCC TGAACTCATA 
CTGCCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTATC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCATTTT AAGTTGTCAC CTACATATGT ATTATATATG 
GCATGTCGGT ATGTATTGTC GAGCCAGTAC AGACCCGATG TCAGTCCTAC AGAGCGTACT 
CACAAAGTGA TTGCCATAGT CAACAAGATG GTGAGCATGA TGGAGGGGGT GATTCAGGAA 
GTAGACCAGG TTGATCAGAA ACAGAAGAAT ATCGCAGGGG CACTTGCTTT CTGGATGGCC 
AATGCATCTG AGCTTCTCAA CTTCATTAAG CAGGACCGAG ATCTTAGTCG AATCACATTG 
GATGCCCAAG ATGTTTTAGC ACACTTGGTT CAAATGGCGT TTAAATATCT GGTTCATTGT 
CTTCAGTCAG AACTGAATAA CTACATGCCA GCTTTTCTGG ATGACCCTGA AGAGAACAGT 
CTACAAAGAC CAAAAATAGA TGACGTCTTG CACACGCTCA CGGGAGCGAT GGCGCTGCTG 
AGGCGCTGCA GGGTCAATGC TGCTCTGACC ATCCAGCTTT TCTCTCAGCT CTTCCACTTC 
ATCAACATGT GGCTCTTCAA CAGATTGGTG ACCGACCCCG AGTCGGGACT GTGCTCTCAC 
TACTGGGGGG CAATCATCCG CCAGCAGCTG GGGCACGTGG AAGCCTGGGC AGAGAAGCAG 
GGATTAGAGC TGGCAGCCGA CTGTCACCTG AGCCGGATAG TGCAGGCGAC CACTTTGCTC 
ACCATGGACA AATACGCTCC TGAGGACACC CCAAATATCA ACAGCACCTG CTTTAAGTTA 
AATTCGCTGC AGCTGCAGGC CTTGCTACAG AATTACCACT GTGCGCCCGA CGAGCCCTTT 
GTCCCCACGG ACCTGATAGA GAATGTCGTG GCCGTGGCTG AAAACACGGC CGACGAGCTG 
GCACGCAGTG ATGGGCGGGA GGTGCAGCTG GAAGAGGACC CTGATCTGCA GCTGCCTTTT 
CTTTTGCCGG AAGACGGCTA TTCTTGTGAC GTCGTCAGGA ACGTTCCAAA CGGTTTACAA 
GAATTTCTGG ACCCTCTGTG CCAGAGAGGG TTTTGCAGGT TAATTCCTCA TGCACGTTCA 
CCAGGTACTT GGACGATATA TTTTGAAGGT GCAGATTATG AGAGCCATCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGCGGAAA GAACCTGAAA TAATCACTGT GACTCTCAAA 
AAGCAAAACG GAATGGGCCT CAGCATTGTC GCAGCAAAGG GTGCTGGTCA AGATAAACTT 
GGAATCTATG TCAAGTCTGT CGTAAAAGGA GGTGCTGCAG ATGTGGATGG ACGCCTAGCC 
GCAGGTGACC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TAGGACTCTC TCAAGAAAGG 
GCAGCAGAAC TCATGACAAG GACCAGTTCC GTGGTGACAC TGGAAGTAGC CAAGCAAGGA 
GCGATCTACC ACGGCCTGGC CACCCTGCTC AACCAGCCGT CCCCGATGAT GCAGAGAGTT 
TCAGACCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGCG AAGGTTTCGA GCTCTATAAC 
AATTCAGCTC AAAACGGGTC ACCCGAGAGC CCTCAGCTGC CTTGGGCAGA ATACAGCGAA 
CCGAAGAAGC TCCCTGGCGA TGACAGGCTG ATGAAGAACC GGGCTGACCA CCGCTCCAGC 
CCCAACGTGG CAAATCAGCC CCCCAGTCCC GGAGGGAAAA GCGCGTACGC CCCTGGAGCA 
GCCGCTAAGA TCACGTCCGT CTCCACCGGA AACCTCTGCA CTGAGGAGCA GACCCCTCCG 
CCTCGACCTG AGGCCTACCC CATCCCCACC CAGACATACA CCAGGGAGTA CTTCACCTTC 
CCGGCCTCCA GGTCCCAGGA CCGCGTGGCT CCCCCTCAGA ACCAGTGGCC CAATTACGAG 
GAGAAGCCGC ACGTGCACAC AGATGGTCAT CAGTCCGGCC CCGCAATGCA GCGCGTCACC 
CGCTCCCAGG AGGAACTTCG GGAGGATAAA GCTTACCAAC TCGAGCGGCA TCGGCTGGAG 
GCGGTGCTGG ATCGCAAGTC CGACAGCGAC GTGTGGATCC AGCCGAGCTC CTCGGTGGGC 
TCCAGCACGT CCAGCCAGGA GCACCTGGAC CCCTGCTCCA AGTCGGGCAC CCCCGCCTCC 
ACGCTGACCA AGAGCGGCCC CGGGCGCTGG AAGACGCCAG CCTCCGCGCA GCCCCTGCCG 
GCGGCCCCCC CGCCCGCGCA GACAGACCTG CCCCCACCAC CCCCGCCGCC CCCCGCGCAC 
TACGCCGGAG ACTTCGACGG GATCCCCGTG GACCTGCCCC TCCCGCCCCC TCCCAGCGGC 
CAGGCGGCGG CGGAGCGGAG GAAACGGGAG GAGCAGCAGC GCTGGTACGA GAAGGAGAAG 
GCCCGCCTGG AGGAGGAGCG GGACAGGAAG CGCCGGGAGC AGGAGCGGAA GCTGGGCCAG 
ATGCGCTCCC AGGCCCTGAA CCCGGCCGCG TTCTCCCCAG TGACCGTCGC GCACACGAAG 
CCGGAGAAAC CTTCCACGCT CCAGAGGCCC CAGGACACGG TCATCCGGGA GCTGCAGCCC 
CAGCAGCAGC CACGCACCAT CGAGCGCCGA GACTTGCAGT ACATCACGGT CAGCAAAGAG 
GAGCTGTCCT CGGGGGACAG CCTGTCCCCC GACCCCTGGA AGCGGGACGC CAGGGAGAAG 
CTGGAGAAGC AGCAGCAAAT GCACATCGTG GACATGCTGA GCAGGGAGAT CCGGGAGCTC 
CAGAGCAAGC CCGAGCGCAG CGCCGAGGAC AGCGACCGCC TGCGCAAGCT CATGCTGGAG 
TGGCAGTTCC AGAAGAGACT GCAGGAGTCC AAGCAGAAGG ACGAGGACGA CGAGGAGGAG 
GAGGACGACG ACGTGGACAC CATGCTGATC ATGCAGCGCC TCGAGGCTGA GCGGAGAGCA 
AGGCAGACTG GTCTGCCAGC AATCTCTGTT CTAGATTTGT TACAGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGTCAGGAC AAGCTCTCCC ACACCGGACC AAACTCTTCC 
GTGGGATCTT CCGGGGCAGC GATCGGTGCC CGTGACGGCT CCCGGGACCC GAGAGAGAGG 
CTGGCGAAAA GCCATGATGC AGATTTACCT GGAAATTCCG GAGCCCCTGA GAACTTGACA 
TTCAAAGAGC GCCAGCGGCT GTTCTCACAA GGTCAAGATG TGTCCAATAA AGTGAAAGCC 
TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831501.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687060.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OFb43237
Clone ID Related Accession (Same CDS sequence)	XM_019831501.1
Accession Version	XM_019831501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGAAACA GAAGAATATC GCAGGGGCAC TTGCTTTCTG GATGGCCAAT 
GCATCTGAGC TTCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTTTAGCACA CTTGGTTCAA ATGGCGTTTA AATATCTGGT TCATTGTCTT 
CAGTCAGAAC TGAATAACTA CATGCCAGCT TTTCTGGATG ACCCTGAAGA GAACAGTCTA 
CAAAGACCAA AAATAGATGA CGTCTTGCAC ACGCTCACGG GAGCGATGGC GCTGCTGAGG 
CGCTGCAGGG TCAATGCTGC TCTGACCATC CAGCTTTTCT CTCAGCTCTT CCACTTCATC 
AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCCGAGT CGGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGG CACGTGGAAG CCTGGGCAGA GAAGCAGGGA 
TTAGAGCTGG CAGCCGACTG TCACCTGAGC CGGATAGTGC AGGCGACCAC TTTGCTCACC 
ATGGACAAAT ACGCTCCTGA GGACACCCCA AATATCAACA GCACCTGCTT TAAGTTAAAT 
TCGCTGCAGC TGCAGGCCTT GCTACAGAAT TACCACTGTG CGCCCGACGA GCCCTTTGTC 
CCCACGGACC TGATAGAGAA TGTCGTGGCC GTGGCTGAAA ACACGGCCGA CGAGCTGGCA 
CGCAGTGATG GGCGGGAGGT GCAGCTGGAA GAGGACCCTG ATCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGCTATTC TTGTGACGTC GTCAGGAACG TTCCAAACGG TTTACAAGAA 
TTTCTGGACC CTCTGTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCATGC ACGTTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCATCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GCGGAAAGAA CCTGAAATAA TCACTGTGAC TCTCAAAAAG 
CAAAACGGAA TGGGCCTCAG CATTGTCGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA 
ATCTATGTCA AGTCTGTCGT AAAAGGAGGT GCTGCAGATG TGGATGGACG CCTAGCCGCA 
GGTGACCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCA 
GCAGAACTCA TGACAAGGAC CAGTTCCGTG GTGACACTGG AAGTAGCCAA GCAAGGAGCG 
ATCTACCACG GCCTGGCCAC CCTGCTCAAC CAGCCGTCCC CGATGATGCA GAGAGTTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTCGAGCT CTATAACAAT 
TCAGCTCAAA ACGGGTCACC CGAGAGCCCT CAGCTGCCTT GGGCAGAATA CAGCGAACCG 
AAGAAGCTCC CTGGCGATGA CAGGCTGATG AAGAACCGGG CTGACCACCG CTCCAGCCCC 
AACGTGGCAA ATCAGCCCCC CAGTCCCGGA GGGAAAAGCG CGTACGCCCC TGGAGCAGCC 
GCTAAGATCA CGTCCGTCTC CACCGGAAAC CTCTGCACTG AGGAGCAGAC CCCTCCGCCT 
CGACCTGAGG CCTACCCCAT CCCCACCCAG ACATACACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGCTCCC CCTCAGAACC AGTGGCCCAA TTACGAGGAG 
AAGCCGCACG TGCACACAGA TGGTCATCAG TCCGGCCCCG CAATGCAGCG CGTCACCCGC 
TCCCAGGAGG AACTTCGGGA GGATAAAGCT TACCAACTCG AGCGGCATCG GCTGGAGGCG 
GTGCTGGATC GCAAGTCCGA CAGCGACGTG TGGATCCAGC CGAGCTCCTC GGTGGGCTCC 
AGCACGTCCA GCCAGGAGCA CCTGGACCCC TGCTCCAAGT CGGGCACCCC CGCCTCCACG 
CTGACCAAGA GCGGCCCCGG GCGCTGGAAG ACGCCAGCCT CCGCGCAGCC CCTGCCGGCG 
GCCCCCCCGC CCGCGCAGAC AGACCTGCCC CCACCACCCC CGCCGCCCCC CGCGCACTAC 
GCCGGAGACT TCGACGGGAT CCCCGTGGAC CTGCCCCTCC CGCCCCCTCC CAGCGGCCAG 
GCGGCGGCGG AGCGGAGGAA ACGGGAGGAG CAGCAGCGCT GGTACGAGAA GGAGAAGGCC 
CGCCTGGAGG AGGAGCGGGA CAGGAAGCGC CGGGAGCAGG AGCGGAAGCT GGGCCAGATG 
CGCTCCCAGG CCCTGAACCC GGCCGCGTTC TCCCCAGTGA CCGTCGCGCA CACGAAGCCG 
GAGAAACCTT CCACGCTCCA GAGGCCCCAG GACACGGTCA TCCGGGAGCT GCAGCCCCAG 
CAGCAGCCAC GCACCATCGA GCGCCGAGAC TTGCAGTACA TCACGGTCAG CAAAGAGGAG 
CTGTCCTCGG GGGACAGCCT GTCCCCCGAC CCCTGGAAGC GGGACGCCAG GGAGAAGCTG 
GAGAAGCAGC AGCAAATGCA CATCGTGGAC ATGCTGAGCA GGGAGATCCG GGAGCTCCAG 
AGCAAGCCCG AGCGCAGCGC CGAGGACAGC GACCGCCTGC GCAAGCTCAT GCTGGAGTGG 
CAGTTCCAGA AGAGACTGCA GGAGTCCAAG CAGAAGGACG AGGACGACGA GGAGGAGGAG 
GACGACGACG TGGACACCAT GCTGATCATG CAGCGCCTCG AGGCTGAGCG GAGAGCAAGG 
TTACAGGACG AGGAGCGGCG GCGGCAGCGG CAGCTGGACG ACATGCGCAG GCGGGAAGCG 
GAGGACCGCG CCAGGCAGGA GGAGGAGAGG ACGAAGCGAG ACGCCGAAGA CAAGAGGCGA 
CAGGAAGAAG GTTATTACAG CCGCCTGGAA GCCGAGAGGC GCAGACAGCA CGCGGAGGCC 
GAGCGCAGGC TGCTGGAGCC GGAGGAGCCG GGCCTGGGCA GACCGCCGCT GCCGCGGGGC 
TACGAGCTCC CGGCCCCGTC CTCCCCGGCG GCTGCTCCTC CTCCCCCACC TCAGCGGACC 
GCCTCCTCCC TGCAGGCCCC GGCCCTGTCC CCAGACTCCC TGTACACCGC CAGGCTCGTT 
GCGTACCACG AGGAGGAGGA GGAGGAGGAC TGCAGTCTGG CAGGACCAAA CTCTTCCGTG 
GGATCTTCCG GGGCAGCGAT CGGTGCCCGT GACGGCTCCC GGGACCCGAG AGAGAGGCTG 
GCGAAAAGCC ATGATGCAGA TTTACCTGGA AATTCCGGAG CCCCTGAGAA CTTGACATTC 
AAAGAGCGCC AGCGGCTGTT CTCACAAGGT CAAGATGTGT CCAATAAAGT GAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687060.1
CDS	22..5343
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831501.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831501.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGGAAGTA 
GACCAGGTTG ATCAGAAACA GAAGAATATC GCAGGGGCAC TTGCTTTCTG GATGGCCAAT 
GCATCTGAGC TTCTCAACTT CATTAAGCAG GACCGAGATC TTAGTCGAAT CACATTGGAT 
GCCCAAGATG TTTTAGCACA CTTGGTTCAA ATGGCGTTTA AATATCTGGT TCATTGTCTT 
CAGTCAGAAC TGAATAACTA CATGCCAGCT TTTCTGGATG ACCCTGAAGA GAACAGTCTA 
CAAAGACCAA AAATAGATGA CGTCTTGCAC ACGCTCACGG GAGCGATGGC GCTGCTGAGG 
CGCTGCAGGG TCAATGCTGC TCTGACCATC CAGCTTTTCT CTCAGCTCTT CCACTTCATC 
AACATGTGGC TCTTCAACAG ATTGGTGACC GACCCCGAGT CGGGACTGTG CTCTCACTAC 
TGGGGGGCAA TCATCCGCCA GCAGCTGGGG CACGTGGAAG CCTGGGCAGA GAAGCAGGGA 
TTAGAGCTGG CAGCCGACTG TCACCTGAGC CGGATAGTGC AGGCGACCAC TTTGCTCACC 
ATGGACAAAT ACGCTCCTGA GGACACCCCA AATATCAACA GCACCTGCTT TAAGTTAAAT 
TCGCTGCAGC TGCAGGCCTT GCTACAGAAT TACCACTGTG CGCCCGACGA GCCCTTTGTC 
CCCACGGACC TGATAGAGAA TGTCGTGGCC GTGGCTGAAA ACACGGCCGA CGAGCTGGCA 
CGCAGTGATG GGCGGGAGGT GCAGCTGGAA GAGGACCCTG ATCTGCAGCT GCCTTTTCTT 
TTGCCGGAAG ACGGCTATTC TTGTGACGTC GTCAGGAACG TTCCAAACGG TTTACAAGAA 
TTTCTGGACC CTCTGTGCCA GAGAGGGTTT TGCAGGTTAA TTCCTCATGC ACGTTCACCA 
GGTACTTGGA CGATATATTT TGAAGGTGCA GATTATGAGA GCCATCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GCGGAAAGAA CCTGAAATAA TCACTGTGAC TCTCAAAAAG 
CAAAACGGAA TGGGCCTCAG CATTGTCGCA GCAAAGGGTG CTGGTCAAGA TAAACTTGGA 
ATCTATGTCA AGTCTGTCGT AAAAGGAGGT GCTGCAGATG TGGATGGACG CCTAGCCGCA 
GGTGACCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTAG GACTCTCTCA AGAAAGGGCA 
GCAGAACTCA TGACAAGGAC CAGTTCCGTG GTGACACTGG AAGTAGCCAA GCAAGGAGCG 
ATCTACCACG GCCTGGCCAC CCTGCTCAAC CAGCCGTCCC CGATGATGCA GAGAGTTTCA 
GACCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGCGAAG GTTTCGAGCT CTATAACAAT 
TCAGCTCAAA ACGGGTCACC CGAGAGCCCT CAGCTGCCTT GGGCAGAATA CAGCGAACCG 
AAGAAGCTCC CTGGCGATGA CAGGCTGATG AAGAACCGGG CTGACCACCG CTCCAGCCCC 
AACGTGGCAA ATCAGCCCCC CAGTCCCGGA GGGAAAAGCG CGTACGCCCC TGGAGCAGCC 
GCTAAGATCA CGTCCGTCTC CACCGGAAAC CTCTGCACTG AGGAGCAGAC CCCTCCGCCT 
CGACCTGAGG CCTACCCCAT CCCCACCCAG ACATACACCA GGGAGTACTT CACCTTCCCG 
GCCTCCAGGT CCCAGGACCG CGTGGCTCCC CCTCAGAACC AGTGGCCCAA TTACGAGGAG 
AAGCCGCACG TGCACACAGA TGGTCATCAG TCCGGCCCCG CAATGCAGCG CGTCACCCGC 
TCCCAGGAGG AACTTCGGGA GGATAAAGCT TACCAACTCG AGCGGCATCG GCTGGAGGCG 
GTGCTGGATC GCAAGTCCGA CAGCGACGTG TGGATCCAGC CGAGCTCCTC GGTGGGCTCC 
AGCACGTCCA GCCAGGAGCA CCTGGACCCC TGCTCCAAGT CGGGCACCCC CGCCTCCACG 
CTGACCAAGA GCGGCCCCGG GCGCTGGAAG ACGCCAGCCT CCGCGCAGCC CCTGCCGGCG 
GCCCCCCCGC CCGCGCAGAC AGACCTGCCC CCACCACCCC CGCCGCCCCC CGCGCACTAC 
GCCGGAGACT TCGACGGGAT CCCCGTGGAC CTGCCCCTCC CGCCCCCTCC CAGCGGCCAG 
GCGGCGGCGG AGCGGAGGAA ACGGGAGGAG CAGCAGCGCT GGTACGAGAA GGAGAAGGCC 
CGCCTGGAGG AGGAGCGGGA CAGGAAGCGC CGGGAGCAGG AGCGGAAGCT GGGCCAGATG 
CGCTCCCAGG CCCTGAACCC GGCCGCGTTC TCCCCAGTGA CCGTCGCGCA CACGAAGCCG 
GAGAAACCTT CCACGCTCCA GAGGCCCCAG GACACGGTCA TCCGGGAGCT GCAGCCCCAG 
CAGCAGCCAC GCACCATCGA GCGCCGAGAC TTGCAGTACA TCACGGTCAG CAAAGAGGAG 
CTGTCCTCGG GGGACAGCCT GTCCCCCGAC CCCTGGAAGC GGGACGCCAG GGAGAAGCTG 
GAGAAGCAGC AGCAAATGCA CATCGTGGAC ATGCTGAGCA GGGAGATCCG GGAGCTCCAG 
AGCAAGCCCG AGCGCAGCGC CGAGGACAGC GACCGCCTGC GCAAGCTCAT GCTGGAGTGG 
CAGTTCCAGA AGAGACTGCA GGAGTCCAAG CAGAAGGACG AGGACGACGA GGAGGAGGAG 
GACGACGACG TGGACACCAT GCTGATCATG CAGCGCCTCG AGGCTGAGCG GAGAGCAAGG 
TTACAGGACG AGGAGCGGCG GCGGCAGCGG CAGCTGGACG ACATGCGCAG GCGGGAAGCG 
GAGGACCGCG CCAGGCAGGA GGAGGAGAGG ACGAAGCGAG ACGCCGAAGA CAAGAGGCGA 
CAGGAAGAAG GTTATTACAG CCGCCTGGAA GCCGAGAGGC GCAGACAGCA CGCGGAGGCC 
GAGCGCAGGC TGCTGGAGCC GGAGGAGCCG GGCCTGGGCA GACCGCCGCT GCCGCGGGGC 
TACGAGCTCC CGGCCCCGTC CTCCCCGGCG GCTGCTCCTC CTCCCCCACC TCAGCGGACC 
GCCTCCTCCC TGCAGGCCCC GGCCCTGTCC CCAGACTCCC TGTACACCGC CAGGCTCGTT 
GCGTACCACG AGGAGGAGGA GGAGGAGGAC TGCAGTCTGG CAGGACCAAA CTCTTCCGTG 
GGATCTTCCG GGGCAGCGAT CGGTGCCCGT GACGGCTCCC GGGACCCGAG AGAGAGGCTG 
GCGAAAAGCC ATGATGCAGA TTTACCTGGA AATTCCGGAG CCCCTGAGAA CTTGACATTC 
AAAGAGCGCC AGCGGCTGTT CTCACAAGGT CAAGATGTGT CCAATAAAGT GAAAGCCTCT 
CGTAAATTAA CAGAACTGGA AAACGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831502.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687061.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

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CloneID	OFb43238
Clone ID Related Accession (Same CDS sequence)	XM_019831502.1
Accession Version	XM_019831502.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG 
AAGAATATCG CAGGGGCACT TGCTTTCTGG ATGGCCAATG CATCTGAGCT TCTCAACTTC 
ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACATTGGATG CCCAAGATGT TTTAGCACAC 
TTGGTTCAAA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AGTCAGAACT GAATAACTAC 
ATGCCAGCTT TTCTGGATGA CCCTGAAGAG AACAGTCTAC AAAGACCAAA AATAGATGAC 
GTCTTGCACA CGCTCACGGG AGCGATGGCG CTGCTGAGGC GCTGCAGGGT CAATGCTGCT 
CTGACCATCC AGCTTTTCTC TCAGCTCTTC CACTTCATCA ACATGTGGCT CTTCAACAGA 
TTGGTGACCG ACCCCGAGTC GGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG AAGCAGGGAT TAGAGCTGGC AGCCGACTGT 
CACCTGAGCC GGATAGTGCA GGCGACCACT TTGCTCACCA TGGACAAATA CGCTCCTGAG 
GACACCCCAA ATATCAACAG CACCTGCTTT AAGTTAAATT CGCTGCAGCT GCAGGCCTTG 
CTACAGAATT ACCACTGTGC GCCCGACGAG CCCTTTGTCC CCACGGACCT GATAGAGAAT 
GTCGTGGCCG TGGCTGAAAA CACGGCCGAC GAGCTGGCAC GCAGTGATGG GCGGGAGGTG 
CAGCTGGAAG AGGACCCTGA TCTGCAGCTG CCTTTTCTTT TGCCGGAAGA CGGCTATTCT 
TGTGACGTCG TCAGGAACGT TCCAAACGGT TTACAAGAAT TTCTGGACCC TCTGTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCATGCA CGTTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCATCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
CGGAAAGAAC CTGAAATAAT CACTGTGACT CTCAAAAAGC AAAACGGAAT GGGCCTCAGC 
ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTCGTA 
AAAGGAGGTG CTGCAGATGT GGATGGACGC CTAGCCGCAG GTGACCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCAG CAGAACTCAT GACAAGGACC 
AGTTCCGTGG TGACACTGGA AGTAGCCAAG CAAGGAGCGA TCTACCACGG CCTGGCCACC 
CTGCTCAACC AGCCGTCCCC GATGATGCAG AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTCGAGCTC TATAACAATT CAGCTCAAAA CGGGTCACCC 
GAGAGCCCTC AGCTGCCTTG GGCAGAATAC AGCGAACCGA AGAAGCTCCC TGGCGATGAC 
AGGCTGATGA AGAACCGGGC TGACCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC 
AGTCCCGGAG GGAAAAGCGC GTACGCCCCT GGAGCAGCCG CTAAGATCAC GTCCGTCTCC 
ACCGGAAACC TCTGCACTGA GGAGCAGACC CCTCCGCCTC GACCTGAGGC CTACCCCATC 
CCCACCCAGA CATACACCAG GGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGCTCCCC CTCAGAACCA GTGGCCCAAT TACGAGGAGA AGCCGCACGT GCACACAGAT 
GGTCATCAGT CCGGCCCCGC AATGCAGCGC GTCACCCGCT CCCAGGAGGA ACTTCGGGAG 
GATAAAGCTT ACCAACTCGA GCGGCATCGG CTGGAGGCGG TGCTGGATCG CAAGTCCGAC 
AGCGACGTGT GGATCCAGCC GAGCTCCTCG GTGGGCTCCA GCACGTCCAG CCAGGAGCAC 
CTGGACCCCT GCTCCAAGTC GGGCACCCCC GCCTCCACGC TGACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC CTGCCGGCGG CCCCCCCGCC CGCGCAGACA 
GACCTGCCCC CACCACCCCC GCCGCCCCCC GCGCACTACG CCGGAGACTT CGACGGGATC 
CCCGTGGACC TGCCCCTCCC GCCCCCTCCC AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC 
AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG GGCCAGATGC GCTCCCAGGC CCTGAACCCG 
GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC ACGAAGCCGG AGAAACCTTC CACGCTCCAG 
AGGCCCCAGG ACACGGTCAT CCGGGAGCTG CAGCCCCAGC AGCAGCCACG CACCATCGAG 
CGCCGAGACT TGCAGTACAT CACGGTCAGC AAAGAGGAGC TGTCCTCGGG GGACAGCCTG 
TCCCCCGACC CCTGGAAGCG GGACGCCAGG GAGAAGCTGG AGAAGCAGCA GCAAATGCAC 
ATCGTGGACA TGCTGAGCAG GGAGATCCGG GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC 
GAGGACAGCG ACCGCCTGCG CAAGCTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGACGACGT GGACACCATG 
CTGATCATGC AGCGCCTCGA GGCTGAGCGG AGAGCAAGGT TACAGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGACCAAAC TCTTCCGTGG GATCTTCCGG GGCAGCGATC 
GGTGCCCGTG ACGGCTCCCG GGACCCGAGA GAGAGGCTGG CGAAAAGCCA TGATGCAGAT 
TTACCTGGAA ATTCCGGAGC CCCTGAGAAC TTGACATTCA AAGAGCGCCA GCGGCTGTTC 
TCACAAGGTC AAGATGTGTC CAATAAAGTG AAAGCCTCTC GTAAATTAAC AGAACTGGAA 
AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687061.1
CDS	22..5322
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831502.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831502.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG 
AAGAATATCG CAGGGGCACT TGCTTTCTGG ATGGCCAATG CATCTGAGCT TCTCAACTTC 
ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACATTGGATG CCCAAGATGT TTTAGCACAC 
TTGGTTCAAA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AGTCAGAACT GAATAACTAC 
ATGCCAGCTT TTCTGGATGA CCCTGAAGAG AACAGTCTAC AAAGACCAAA AATAGATGAC 
GTCTTGCACA CGCTCACGGG AGCGATGGCG CTGCTGAGGC GCTGCAGGGT CAATGCTGCT 
CTGACCATCC AGCTTTTCTC TCAGCTCTTC CACTTCATCA ACATGTGGCT CTTCAACAGA 
TTGGTGACCG ACCCCGAGTC GGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG AAGCAGGGAT TAGAGCTGGC AGCCGACTGT 
CACCTGAGCC GGATAGTGCA GGCGACCACT TTGCTCACCA TGGACAAATA CGCTCCTGAG 
GACACCCCAA ATATCAACAG CACCTGCTTT AAGTTAAATT CGCTGCAGCT GCAGGCCTTG 
CTACAGAATT ACCACTGTGC GCCCGACGAG CCCTTTGTCC CCACGGACCT GATAGAGAAT 
GTCGTGGCCG TGGCTGAAAA CACGGCCGAC GAGCTGGCAC GCAGTGATGG GCGGGAGGTG 
CAGCTGGAAG AGGACCCTGA TCTGCAGCTG CCTTTTCTTT TGCCGGAAGA CGGCTATTCT 
TGTGACGTCG TCAGGAACGT TCCAAACGGT TTACAAGAAT TTCTGGACCC TCTGTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCATGCA CGTTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCATCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
CGGAAAGAAC CTGAAATAAT CACTGTGACT CTCAAAAAGC AAAACGGAAT GGGCCTCAGC 
ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTCGTA 
AAAGGAGGTG CTGCAGATGT GGATGGACGC CTAGCCGCAG GTGACCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCAG CAGAACTCAT GACAAGGACC 
AGTTCCGTGG TGACACTGGA AGTAGCCAAG CAAGGAGCGA TCTACCACGG CCTGGCCACC 
CTGCTCAACC AGCCGTCCCC GATGATGCAG AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTCGAGCTC TATAACAATT CAGCTCAAAA CGGGTCACCC 
GAGAGCCCTC AGCTGCCTTG GGCAGAATAC AGCGAACCGA AGAAGCTCCC TGGCGATGAC 
AGGCTGATGA AGAACCGGGC TGACCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC 
AGTCCCGGAG GGAAAAGCGC GTACGCCCCT GGAGCAGCCG CTAAGATCAC GTCCGTCTCC 
ACCGGAAACC TCTGCACTGA GGAGCAGACC CCTCCGCCTC GACCTGAGGC CTACCCCATC 
CCCACCCAGA CATACACCAG GGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGCTCCCC CTCAGAACCA GTGGCCCAAT TACGAGGAGA AGCCGCACGT GCACACAGAT 
GGTCATCAGT CCGGCCCCGC AATGCAGCGC GTCACCCGCT CCCAGGAGGA ACTTCGGGAG 
GATAAAGCTT ACCAACTCGA GCGGCATCGG CTGGAGGCGG TGCTGGATCG CAAGTCCGAC 
AGCGACGTGT GGATCCAGCC GAGCTCCTCG GTGGGCTCCA GCACGTCCAG CCAGGAGCAC 
CTGGACCCCT GCTCCAAGTC GGGCACCCCC GCCTCCACGC TGACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC CTGCCGGCGG CCCCCCCGCC CGCGCAGACA 
GACCTGCCCC CACCACCCCC GCCGCCCCCC GCGCACTACG CCGGAGACTT CGACGGGATC 
CCCGTGGACC TGCCCCTCCC GCCCCCTCCC AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC 
AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG GGCCAGATGC GCTCCCAGGC CCTGAACCCG 
GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC ACGAAGCCGG AGAAACCTTC CACGCTCCAG 
AGGCCCCAGG ACACGGTCAT CCGGGAGCTG CAGCCCCAGC AGCAGCCACG CACCATCGAG 
CGCCGAGACT TGCAGTACAT CACGGTCAGC AAAGAGGAGC TGTCCTCGGG GGACAGCCTG 
TCCCCCGACC CCTGGAAGCG GGACGCCAGG GAGAAGCTGG AGAAGCAGCA GCAAATGCAC 
ATCGTGGACA TGCTGAGCAG GGAGATCCGG GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC 
GAGGACAGCG ACCGCCTGCG CAAGCTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGACGACGT GGACACCATG 
CTGATCATGC AGCGCCTCGA GGCTGAGCGG AGAGCAAGGT TACAGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGACCAAAC TCTTCCGTGG GATCTTCCGG GGCAGCGATC 
GGTGCCCGTG ACGGCTCCCG GGACCCGAGA GAGAGGCTGG CGAAAAGCCA TGATGCAGAT 
TTACCTGGAA ATTCCGGAGC CCCTGAGAAC TTGACATTCA AAGAGCGCCA GCGGCTGTTC 
TCACAAGGTC AAGATGTGTC CAATAAAGTG AAAGCCTCTC GTAAATTAAC AGAACTGGAA 
AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831503.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687062.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

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CloneID	OFb43239
Clone ID Related Accession (Same CDS sequence)	XM_019831503.1
Accession Version	XM_019831503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAGAAGA AGGAGAAAAA GAAGAGAGAG AAAGAGGCAG TGAGGCAGGC ATCTGAGAAA 
GATGATCGGC CTTTCCAAGG GGATGACCTC GAGAATTCCC GACTGGCCGC CGAGGTTTAC 
AAAGACATGC CCGAGACCAG CTTCACTCGC ACGATCTCCA ACCCCGAGGT GGTCATGAAG 
CGCCGGCGGC AGCAGAAGCT GGAGAGGCGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCGGACTCAG GTGGAACACT GAGAATTTAT GCAGATAGCT TAAAACCGAA CATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGATCCT GCTGACTTCG CTGTGGCCGA GGCTCTAGAG 
AAATACGGTC TGGAGAAGGA GAACCCCAAA GATTACTGCA TCGCCCGGGT TATGCTGCCT 
CCTGGAGCCC AGCATTCGGA TGAAAACGGT GCTAAAGAAA CGATCCTCGA TGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAGTGGCCA ACTGACCGAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGGC CGCCAGACTA CGTCCCGAAG AAAACGAAGA AACATGTCGA CGGGAAGCTG 
GTGAAAGGGA AGGAGAGAGC CGAGGGTTCC GGCTATGGCT CAGCACTTCC GCCTGAGAAG 
TTGCCCTACC TGGTGGAGCT CAGCCCAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC TGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACATA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCAATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGCG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCACC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAGAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGT CAGGACAAGC TCTCCCACAC CGGACCAAAC 
TCTTCCGTGG GATCTTCCGG GGCAGCGATC GGTGCCCGTG ACGGCTCCCG GGACCCGAGA 
GAGAGGCTGG CGAAAAGCCA TGATGCAGAT TTACCTGGAA ATTCCGGAGC CCCTGAGAAC 
TTGACATTCA AAGAGCGCCA GCGGCTGTTC TCACAAGGTC AAGATGTGTC CAATAAAGTG 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687062.1
CDS	22..5292
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_019831503.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831503.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGGC TCAAAGCAAT 
GGACCTGAAA AGCAGGAGAA AGAAGGTGTT ATCCAGAACT TCAAGAGAAC TCTCTCCAAG 
AAAGAGAAGA AGGAGAAAAA GAAGAGAGAG AAAGAGGCAG TGAGGCAGGC ATCTGAGAAA 
GATGATCGGC CTTTCCAAGG GGATGACCTC GAGAATTCCC GACTGGCCGC CGAGGTTTAC 
AAAGACATGC CCGAGACCAG CTTCACTCGC ACGATCTCCA ACCCCGAGGT GGTCATGAAG 
CGCCGGCGGC AGCAGAAGCT GGAGAGGCGG ATGCAGGAGT TCCGGAGCTC GGACGGGCGG 
CCGGACTCAG GTGGAACACT GAGAATTTAT GCAGATAGCT TAAAACCGAA CATTCCCTAC 
AAGACAATCC TGCTGTCCAC CACGGATCCT GCTGACTTCG CTGTGGCCGA GGCTCTAGAG 
AAATACGGTC TGGAGAAGGA GAACCCCAAA GATTACTGCA TCGCCCGGGT TATGCTGCCT 
CCTGGAGCCC AGCATTCGGA TGAAAACGGT GCTAAAGAAA CGATCCTCGA TGATGACGAG 
TGTCCTTTAC AGATCTTCAG GGAGTGGCCA ACTGACCGAG GGGTTTTAGT CTTTCAGTTG 
AAGAGGAGGC CGCCAGACTA CGTCCCGAAG AAAACGAAGA AACATGTCGA CGGGAAGCTG 
GTGAAAGGGA AGGAGAGAGC CGAGGGTTCC GGCTATGGCT CAGCACTTCC GCCTGAGAAG 
TTGCCCTACC TGGTGGAGCT CAGCCCAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC TGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACATA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCAATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGCG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCACC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAGAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGT CAGGACAAGC TCTCCCACAC CGGACCAAAC 
TCTTCCGTGG GATCTTCCGG GGCAGCGATC GGTGCCCGTG ACGGCTCCCG GGACCCGAGA 
GAGAGGCTGG CGAAAAGCCA TGATGCAGAT TTACCTGGAA ATTCCGGAGC CCCTGAGAAC 
TTGACATTCA AAGAGCGCCA GCGGCTGTTC TCACAAGGTC AAGATGTGTC CAATAAAGTG 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831504.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687063.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

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CloneID	OFb43240
Clone ID Related Accession (Same CDS sequence)	XM_019831504.1
Accession Version	XM_019831504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG 
AAGAATATCG CAGGGGCACT TGCTTTCTGG ATGGCCAATG CATCTGAGCT TCTCAACTTC 
ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACATTGGATG CCCAAGATGT TTTAGCACAC 
TTGGTTCAAA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AGTCAGAACT GAATAACTAC 
ATGCCAGCTT TTCTGGATGA CCCTGAAGAG AACAGTCTAC AAAGACCAAA AATAGATGAC 
GTCTTGCACA CGCTCACGGG AGCGATGGCG CTGCTGAGGC GCTGCAGGGT CAATGCTGCT 
CTGACCATCC AGCTTTTCTC TCAGCTCTTC CACTTCATCA ACATGTGGCT CTTCAACAGA 
TTGGTGACCG ACCCCGAGTC GGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG AAGCAGGGAT TAGAGCTGGC AGCCGACTGT 
CACCTGAGCC GGATAGTGCA GGCGACCACT TTGCTCACCA TGGACAAATA CGCTCCTGAG 
GACACCCCAA ATATCAACAG CACCTGCTTT AAGTTAAATT CGCTGCAGCT GCAGGCCTTG 
CTACAGAATT ACCACTGTGC GCCCGACGAG CCCTTTGTCC CCACGGACCT GATAGAGAAT 
GTCGTGGCCG TGGCTGAAAA CACGGCCGAC GAGCTGGCAC GCAGTGATGG GCGGGAGGTG 
CAGCTGGAAG AGGACCCTGA TCTGCAGCTG CCTTTTCTTT TGCCGGAAGA CGGCTATTCT 
TGTGACGTCG TCAGGAACGT TCCAAACGGT TTACAAGAAT TTCTGGACCC TCTGTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCATGCA CGTTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCATCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
CGGAAAGAAC CTGAAATAAT CACTGTGACT CTCAAAAAGC AAAACGGAAT GGGCCTCAGC 
ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTCGTA 
AAAGGAGGTG CTGCAGATGT GGATGGACGC CTAGCCGCAG GTGACCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCAG CAGAACTCAT GACAAGGACC 
AGTTCCGTGG TGACACTGGA AGTAGCCAAG CAAGGAGCGA TCTACCACGG CCTGGCCACC 
CTGCTCAACC AGCCGTCCCC GATGATGCAG AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTCGAGCTC TATAACAATT CAGCTCAAAA CGGGTCACCC 
GAGAGCCCTC AGCTGCCTTG GGCAGAATAC AGCGAACCGA AGAAGCTCCC TGGCGATGAC 
AGGCTGATGA AGAACCGGGC TGACCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC 
AGTCCCGGAG GGAAAAGCGC GTACGCCCCT GGAGCAGCCG CTAAGATCAC GTCCGTCTCC 
ACCGGAAACC TCTGCACTGA GGAGCAGACC CCTCCGCCTC GACCTGAGGC CTACCCCATC 
CCCACCCAGA CATACACCAG GGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGCTCCCC CTCAGAACCA GTGGCCCAAT TACGAGGAGA AGCCGCACGT GCACACAGAT 
GGTCATCAGT CCGGCCCCGC AATGCAGCGC GTCACCCGCT CCCAGGAGGA ACTTCGGGAG 
GATAAAGCTT ACCAACTCGA GCGGCATCGG CTGGAGGCGG TGCTGGATCG CAAGTCCGAC 
AGCGACGTGT GGATCCAGCC GAGCTCCTCG GTGGGCTCCA GCACGTCCAG CCAGGAGCAC 
CTGGACCCCT GCTCCAAGTC GGGCACCCCC GCCTCCACGC TGACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC CTGCCGGCGG CCCCCCCGCC CGCGCAGACA 
GACCTGCCCC CACCACCCCC GCCGCCCCCC GCGCACTACG CCGGAGACTT CGACGGGATC 
CCCGTGGACC TGCCCCTCCC GCCCCCTCCC AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC 
AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG GGCCAGATGC GCTCCCAGGC CCTGAACCCG 
GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC ACGAAGCCGG AGAAACCTTC CACGCTCCAG 
AGGCCCCAGG ACACGGTCAT CCGGGAGCTG CAGCCCCAGC AGCAGCCACG CACCATCGAG 
CGCCGAGACT TGCAGTACAT CACGGTCAGC AAAGAGGAGC TGTCCTCGGG GGACAGCCTG 
TCCCCCGACC CCTGGAAGCG GGACGCCAGG GAGAAGCTGG AGAAGCAGCA GCAAATGCAC 
ATCGTGGACA TGCTGAGCAG GGAGATCCGG GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC 
GAGGACAGCG ACCGCCTGCG CAAGCTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGACGACGT GGACACCATG 
CTGATCATGC AGCGCCTCGA GGCTGAGCGG AGAGCAAGGC AGACTGGTCT GCCAGCAATC 
TCTGTTCTAG ATTTGTTACA GGACGAGGAG CGGCGGCGGC AGCGGCAGCT GGACGACATG 
CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG CAGGAGGAGG AGAGGACGAA GCGAGACGCC 
GAAGACAAGG TCATGGTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687063.1
CDS	22..4845
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKVMVL

Target ORF information:

RefSeq Version	XM_019831504.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831504.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG 
AAGAATATCG CAGGGGCACT TGCTTTCTGG ATGGCCAATG CATCTGAGCT TCTCAACTTC 
ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACATTGGATG CCCAAGATGT TTTAGCACAC 
TTGGTTCAAA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AGTCAGAACT GAATAACTAC 
ATGCCAGCTT TTCTGGATGA CCCTGAAGAG AACAGTCTAC AAAGACCAAA AATAGATGAC 
GTCTTGCACA CGCTCACGGG AGCGATGGCG CTGCTGAGGC GCTGCAGGGT CAATGCTGCT 
CTGACCATCC AGCTTTTCTC TCAGCTCTTC CACTTCATCA ACATGTGGCT CTTCAACAGA 
TTGGTGACCG ACCCCGAGTC GGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG AAGCAGGGAT TAGAGCTGGC AGCCGACTGT 
CACCTGAGCC GGATAGTGCA GGCGACCACT TTGCTCACCA TGGACAAATA CGCTCCTGAG 
GACACCCCAA ATATCAACAG CACCTGCTTT AAGTTAAATT CGCTGCAGCT GCAGGCCTTG 
CTACAGAATT ACCACTGTGC GCCCGACGAG CCCTTTGTCC CCACGGACCT GATAGAGAAT 
GTCGTGGCCG TGGCTGAAAA CACGGCCGAC GAGCTGGCAC GCAGTGATGG GCGGGAGGTG 
CAGCTGGAAG AGGACCCTGA TCTGCAGCTG CCTTTTCTTT TGCCGGAAGA CGGCTATTCT 
TGTGACGTCG TCAGGAACGT TCCAAACGGT TTACAAGAAT TTCTGGACCC TCTGTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCATGCA CGTTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCATCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
CGGAAAGAAC CTGAAATAAT CACTGTGACT CTCAAAAAGC AAAACGGAAT GGGCCTCAGC 
ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTCGTA 
AAAGGAGGTG CTGCAGATGT GGATGGACGC CTAGCCGCAG GTGACCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCAG CAGAACTCAT GACAAGGACC 
AGTTCCGTGG TGACACTGGA AGTAGCCAAG CAAGGAGCGA TCTACCACGG CCTGGCCACC 
CTGCTCAACC AGCCGTCCCC GATGATGCAG AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTCGAGCTC TATAACAATT CAGCTCAAAA CGGGTCACCC 
GAGAGCCCTC AGCTGCCTTG GGCAGAATAC AGCGAACCGA AGAAGCTCCC TGGCGATGAC 
AGGCTGATGA AGAACCGGGC TGACCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC 
AGTCCCGGAG GGAAAAGCGC GTACGCCCCT GGAGCAGCCG CTAAGATCAC GTCCGTCTCC 
ACCGGAAACC TCTGCACTGA GGAGCAGACC CCTCCGCCTC GACCTGAGGC CTACCCCATC 
CCCACCCAGA CATACACCAG GGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGCTCCCC CTCAGAACCA GTGGCCCAAT TACGAGGAGA AGCCGCACGT GCACACAGAT 
GGTCATCAGT CCGGCCCCGC AATGCAGCGC GTCACCCGCT CCCAGGAGGA ACTTCGGGAG 
GATAAAGCTT ACCAACTCGA GCGGCATCGG CTGGAGGCGG TGCTGGATCG CAAGTCCGAC 
AGCGACGTGT GGATCCAGCC GAGCTCCTCG GTGGGCTCCA GCACGTCCAG CCAGGAGCAC 
CTGGACCCCT GCTCCAAGTC GGGCACCCCC GCCTCCACGC TGACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC CTGCCGGCGG CCCCCCCGCC CGCGCAGACA 
GACCTGCCCC CACCACCCCC GCCGCCCCCC GCGCACTACG CCGGAGACTT CGACGGGATC 
CCCGTGGACC TGCCCCTCCC GCCCCCTCCC AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC 
AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG GGCCAGATGC GCTCCCAGGC CCTGAACCCG 
GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC ACGAAGCCGG AGAAACCTTC CACGCTCCAG 
AGGCCCCAGG ACACGGTCAT CCGGGAGCTG CAGCCCCAGC AGCAGCCACG CACCATCGAG 
CGCCGAGACT TGCAGTACAT CACGGTCAGC AAAGAGGAGC TGTCCTCGGG GGACAGCCTG 
TCCCCCGACC CCTGGAAGCG GGACGCCAGG GAGAAGCTGG AGAAGCAGCA GCAAATGCAC 
ATCGTGGACA TGCTGAGCAG GGAGATCCGG GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC 
GAGGACAGCG ACCGCCTGCG CAAGCTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGACGACGT GGACACCATG 
CTGATCATGC AGCGCCTCGA GGCTGAGCGG AGAGCAAGGC AGACTGGTCT GCCAGCAATC 
TCTGTTCTAG ATTTGTTACA GGACGAGGAG CGGCGGCGGC AGCGGCAGCT GGACGACATG 
CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG CAGGAGGAGG AGAGGACGAA GCGAGACGCC 
GAAGACAAGG TCATGGTCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_019831505.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_019687064.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.

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CloneID	OFb43241
Clone ID Related Accession (Same CDS sequence)	XM_019831505.1
Accession Version	XM_019831505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1482854400000
Organism	Felis catus(Domestic cat)
Product	afadin isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Felis catus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG 
AAGAATATCG CAGGGGCACT TGCTTTCTGG ATGGCCAATG CATCTGAGCT TCTCAACTTC 
ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACATTGGATG CCCAAGATGT TTTAGCACAC 
TTGGTTCAAA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AGTCAGAACT GAATAACTAC 
ATGCCAGCTT TTCTGGATGA CCCTGAAGAG AACAGTCTAC AAAGACCAAA AATAGATGAC 
GTCTTGCACA CGCTCACGGG AGCGATGGCG CTGCTGAGGC GCTGCAGGGT CAATGCTGCT 
CTGACCATCC AGCTTTTCTC TCAGCTCTTC CACTTCATCA ACATGTGGCT CTTCAACAGA 
TTGGTGACCG ACCCCGAGTC GGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG AAGCAGGGAT TAGAGCTGGC AGCCGACTGT 
CACCTGAGCC GGATAGTGCA GGCGACCACT TTGCTCACCA TGGACAAATA CGCTCCTGAG 
GACACCCCAA ATATCAACAG CACCTGCTTT AAGTTAAATT CGCTGCAGCT GCAGGCCTTG 
CTACAGAATT ACCACTGTGC GCCCGACGAG CCCTTTGTCC CCACGGACCT GATAGAGAAT 
GTCGTGGCCG TGGCTGAAAA CACGGCCGAC GAGCTGGCAC GCAGTGATGG GCGGGAGGTG 
CAGCTGGAAG AGGACCCTGA TCTGCAGCTG CCTTTTCTTT TGCCGGAAGA CGGCTATTCT 
TGTGACGTCG TCAGGAACGT TCCAAACGGT TTACAAGAAT TTCTGGACCC TCTGTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCATGCA CGTTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCATCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
CGGAAAGAAC CTGAAATAAT CACTGTGACT CTCAAAAAGC AAAACGGAAT GGGCCTCAGC 
ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTCGTA 
AAAGGAGGTG CTGCAGATGT GGATGGACGC CTAGCCGCAG GTGACCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCAG CAGAACTCAT GACAAGGACC 
AGTTCCGTGG TGACACTGGA AGTAGCCAAG CAAGGAGCGA TCTACCACGG CCTGGCCACC 
CTGCTCAACC AGCCGTCCCC GATGATGCAG AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTCGAGCTC TATAACAATT CAGCTCAAAA CGGGTCACCC 
GAGAGCCCTC AGCTGCCTTG GGCAGAATAC AGCGAACCGA AGAAGCTCCC TGGCGATGAC 
AGGCTGATGA AGAACCGGGC TGACCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC 
AGTCCCGGAG GGAAAAGCGC GTACGCCCCT GGAGCAGCCG CTAAGATCAC GTCCGTCTCC 
ACCGGAAACC TCTGCACTGA GGAGCAGACC CCTCCGCCTC GACCTGAGGC CTACCCCATC 
CCCACCCAGA CATACACCAG GGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGCTCCCC CTCAGAACCA GTGGCCCAAT TACGAGGAGA AGCCGCACGT GCACACAGAT 
GGTCATCAGT CCGGCCCCGC AATGCAGCGC GTCACCCGCT CCCAGGAGGA ACTTCGGGAG 
GATAAAGCTT ACCAACTCGA GCGGCATCGG CTGGAGGCGG TGCTGGATCG CAAGTCCGAC 
AGCGACGTGT GGATCCAGCC GAGCTCCTCG GTGGGCTCCA GCACGTCCAG CCAGGAGCAC 
CTGGACCCCT GCTCCAAGTC GGGCACCCCC GCCTCCACGC TGACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC CTGCCGGCGG CCCCCCCGCC CGCGCAGACA 
GACCTGCCCC CACCACCCCC GCCGCCCCCC GCGCACTACG CCGGAGACTT CGACGGGATC 
CCCGTGGACC TGCCCCTCCC GCCCCCTCCC AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC 
AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG GGCCAGATGC GCTCCCAGGC CCTGAACCCG 
GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC ACGAAGCCGG AGAAACCTTC CACGCTCCAG 
AGGCCCCAGG ACACGGTCAT CCGGGAGCTG CAGCCCCAGC AGCAGCCACG CACCATCGAG 
CGCCGAGACT TGCAGTACAT CACGGTCAGC AAAGAGGAGC TGTCCTCGGG GGACAGCCTG 
TCCCCCGACC CCTGGAAGCG GGACGCCAGG GAGAAGCTGG AGAAGCAGCA GCAAATGCAC 
ATCGTGGACA TGCTGAGCAG GGAGATCCGG GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC 
GAGGACAGCG ACCGCCTGCG CAAGCTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGACGACGT GGACACCATG 
CTGATCATGC AGCGCCTCGA GGCTGAGCGG AGAGCAAGGG ACGAGGAGCG GCGGCGGCAG 
CGGCAGCTGG ACGACATGCG CAGGCGGGAA GCGGAGGACC GCGCCAGGCA GGAGGAGGAG 
AGGACGAAGC GAGACGCCGA AGACAAGGTC ATGGTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019687064.1
CDS	22..4803
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKVMVL

Target ORF information:

RefSeq Version	XM_019831505.1
Organism	Felis catus(Domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019831505.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG CATTCGGGTC 
TCTAGTACGG CCACCACGCA AGACGTGATC GAGACGCTGG CAGAGAAATT CCGCCCTGAC 
ATGCGGATGC TGTCGTCCCC CAAGTACTCC CTCTACGAAG TCCACGTCAG CGGAGAAAGA 
AGATTGGATG TAGATGAGAA ACCCCTTGTT GTGCAGTTGA ATTGGAATAA AGACGATCGA 
GAGGGCAGAT TTGTTCTCAA GAATGAGAAC GATGCCCTTC CTCCTAAGAA GGCTCAAAGC 
AATGGACCTG AAAAGCAGGA GAAAGAAGGT GTTATCCAGA ACTTCAAGAG AACTCTCTCC 
AAGAAAGAGA AGAAGGAGAA AAAGAAGAGA GAGAAAGAGG CAGTGAGGCA GGCATCTGAG 
AAAGATGATC GGCCTTTCCA AGGGGATGAC CTCGAGAATT CCCGACTGGC CGCCGAGGTT 
TACAAAGACA TGCCCGAGAC CAGCTTCACT CGCACGATCT CCAACCCCGA GGTGGTCATG 
AAGCGCCGGC GGCAGCAGAA GCTGGAGAGG CGGATGCAGG AGTTCCGGAG CTCGGACGGG 
CGGCCGGACT CAGGTGGAAC ACTGAGAATT TATGCAGATA GCTTAAAACC GAACATTCCC 
TACAAGACAA TCCTGCTGTC CACCACGGAT CCTGCTGACT TCGCTGTGGC CGAGGCTCTA 
GAGAAATACG GTCTGGAGAA GGAGAACCCC AAAGATTACT GCATCGCCCG GGTTATGCTG 
CCTCCTGGAG CCCAGCATTC GGATGAAAAC GGTGCTAAAG AAACGATCCT CGATGATGAC 
GAGTGTCCTT TACAGATCTT CAGGGAGTGG CCAACTGACC GAGGGGTTTT AGTCTTTCAG 
TTGAAGAGGA GGCCGCCAGA CTACGTCCCG AAGAAAACGA AGAAACATGT CGACGGGAAG 
CTGGTGAAAG GGAAGGAGAG AGCCGAGGGT TCCGGCTATG GCTCAGCACT TCCGCCTGAG 
AAGTTGCCCT ACCTGGTGGA GCTCAGCCCA GTCCCAGGGA GAAGGAGTCA CTTTGCCTAC 
TACAGCTATC ACACTTACGA AGATGGTTCT GACTCCAGGG ACAAGCCCAA GCTTTACCGC 
CTTCAGTTGA GCGTTACTGA GGTCGGGACA GAGAAGTTCG AGGAGAACTC CATCCAGCTG 
TTTGGATCAG GAATTCAGCC CCATCACTGT GACCTCACAA ACATGGATGG AGTTGTCACC 
GTGACTCCGA GAAGCATGGA CGCCGAGACG TACGTGGAAG GACAGCGCAT CTCCGAGACA 
ACCATGCTGC AGAGTGGGAT GAAAGTGCAG TTTGGGGCGT CACACGTGTT CAAGTTTGTG 
GACCCCAGCC AGGACCACGC AGTGGCCAAG AGACCTGTGG ATGGAGGCCT GACGGCCAAG 
GGCCCCAGAC AGAAGCCTGG AATTGTTCAG GAGACAACTT TCGATTTGGG GGGAGATGTT 
CACAGCGGGG CAGCGCTGCC AGCGAGCAAG AGCGCCACCA GGCTGGACGG CGACAGGGCA 
CTGCCTGCCC CCAGCACGGC CGAGCGCGGG ACAGTGAAGC CGATGCTGAG ACTGGAGCAG 
CAGGACTACC GCCGGCAAGA AAACAGAACT CAGGATGCTC TTGGGCCTGA ACTCATACTG 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTATCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCATTTTAAG TTGTCACCTA CATATGTATT ATATATGGCA 
TGTCGGTATG TATTGTCGAG CCAGTACAGA CCCGATGTCA GTCCTACAGA GCGTACTCAC 
AAAGTGATTG CCATAGTCAA CAAGATGGTG AGCATGATGG AGGGGGTGAT TCAGAAACAG 
AAGAATATCG CAGGGGCACT TGCTTTCTGG ATGGCCAATG CATCTGAGCT TCTCAACTTC 
ATTAAGCAGG ACCGAGATCT TAGTCGAATC ACATTGGATG CCCAAGATGT TTTAGCACAC 
TTGGTTCAAA TGGCGTTTAA ATATCTGGTT CATTGTCTTC AGTCAGAACT GAATAACTAC 
ATGCCAGCTT TTCTGGATGA CCCTGAAGAG AACAGTCTAC AAAGACCAAA AATAGATGAC 
GTCTTGCACA CGCTCACGGG AGCGATGGCG CTGCTGAGGC GCTGCAGGGT CAATGCTGCT 
CTGACCATCC AGCTTTTCTC TCAGCTCTTC CACTTCATCA ACATGTGGCT CTTCAACAGA 
TTGGTGACCG ACCCCGAGTC GGGACTGTGC TCTCACTACT GGGGGGCAAT CATCCGCCAG 
CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG AAGCAGGGAT TAGAGCTGGC AGCCGACTGT 
CACCTGAGCC GGATAGTGCA GGCGACCACT TTGCTCACCA TGGACAAATA CGCTCCTGAG 
GACACCCCAA ATATCAACAG CACCTGCTTT AAGTTAAATT CGCTGCAGCT GCAGGCCTTG 
CTACAGAATT ACCACTGTGC GCCCGACGAG CCCTTTGTCC CCACGGACCT GATAGAGAAT 
GTCGTGGCCG TGGCTGAAAA CACGGCCGAC GAGCTGGCAC GCAGTGATGG GCGGGAGGTG 
CAGCTGGAAG AGGACCCTGA TCTGCAGCTG CCTTTTCTTT TGCCGGAAGA CGGCTATTCT 
TGTGACGTCG TCAGGAACGT TCCAAACGGT TTACAAGAAT TTCTGGACCC TCTGTGCCAG 
AGAGGGTTTT GCAGGTTAAT TCCTCATGCA CGTTCACCAG GTACTTGGAC GATATATTTT 
GAAGGTGCAG ATTATGAGAG CCATCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
CGGAAAGAAC CTGAAATAAT CACTGTGACT CTCAAAAAGC AAAACGGAAT GGGCCTCAGC 
ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT AAACTTGGAA TCTATGTCAA GTCTGTCGTA 
AAAGGAGGTG CTGCAGATGT GGATGGACGC CTAGCCGCAG GTGACCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTAGG ACTCTCTCAA GAAAGGGCAG CAGAACTCAT GACAAGGACC 
AGTTCCGTGG TGACACTGGA AGTAGCCAAG CAAGGAGCGA TCTACCACGG CCTGGCCACC 
CTGCTCAACC AGCCGTCCCC GATGATGCAG AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGCGAAGG TTTCGAGCTC TATAACAATT CAGCTCAAAA CGGGTCACCC 
GAGAGCCCTC AGCTGCCTTG GGCAGAATAC AGCGAACCGA AGAAGCTCCC TGGCGATGAC 
AGGCTGATGA AGAACCGGGC TGACCACCGC TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC 
AGTCCCGGAG GGAAAAGCGC GTACGCCCCT GGAGCAGCCG CTAAGATCAC GTCCGTCTCC 
ACCGGAAACC TCTGCACTGA GGAGCAGACC CCTCCGCCTC GACCTGAGGC CTACCCCATC 
CCCACCCAGA CATACACCAG GGAGTACTTC ACCTTCCCGG CCTCCAGGTC CCAGGACCGC 
GTGGCTCCCC CTCAGAACCA GTGGCCCAAT TACGAGGAGA AGCCGCACGT GCACACAGAT 
GGTCATCAGT CCGGCCCCGC AATGCAGCGC GTCACCCGCT CCCAGGAGGA ACTTCGGGAG 
GATAAAGCTT ACCAACTCGA GCGGCATCGG CTGGAGGCGG TGCTGGATCG CAAGTCCGAC 
AGCGACGTGT GGATCCAGCC GAGCTCCTCG GTGGGCTCCA GCACGTCCAG CCAGGAGCAC 
CTGGACCCCT GCTCCAAGTC GGGCACCCCC GCCTCCACGC TGACCAAGAG CGGCCCCGGG 
CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC CTGCCGGCGG CCCCCCCGCC CGCGCAGACA 
GACCTGCCCC CACCACCCCC GCCGCCCCCC GCGCACTACG CCGGAGACTT CGACGGGATC 
CCCGTGGACC TGCCCCTCCC GCCCCCTCCC AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA 
CGGGAGGAGC AGCAGCGCTG GTACGAGAAG GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC 
AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG GGCCAGATGC GCTCCCAGGC CCTGAACCCG 
GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC ACGAAGCCGG AGAAACCTTC CACGCTCCAG 
AGGCCCCAGG ACACGGTCAT CCGGGAGCTG CAGCCCCAGC AGCAGCCACG CACCATCGAG 
CGCCGAGACT TGCAGTACAT CACGGTCAGC AAAGAGGAGC TGTCCTCGGG GGACAGCCTG 
TCCCCCGACC CCTGGAAGCG GGACGCCAGG GAGAAGCTGG AGAAGCAGCA GCAAATGCAC 
ATCGTGGACA TGCTGAGCAG GGAGATCCGG GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC 
GAGGACAGCG ACCGCCTGCG CAAGCTCATG CTGGAGTGGC AGTTCCAGAA GAGACTGCAG 
GAGTCCAAGC AGAAGGACGA GGACGACGAG GAGGAGGAGG ACGACGACGT GGACACCATG 
CTGATCATGC AGCGCCTCGA GGCTGAGCGG AGAGCAAGGG ACGAGGAGCG GCGGCGGCAG 
CGGCAGCTGG ACGACATGCG CAGGCGGGAA GCGGAGGACC GCGCCAGGCA GGAGGAGGAG 
AGGACGAAGC GAGACGCCGA AGACAAGGTC ATGGTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254578.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110346.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

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CloneID	OFb76137
Clone ID Related Accession (Same CDS sequence)	XM_023254578.1
Accession Version	XM_023254578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGC AGACTGGTCT GCCAGCAATC TCTGTTCTAG ATTTGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGT CAGGACAAGC TCTCCCACAC CGGACCAAAC 
TCTTCCGTGG GATCTTCCGG GGCAGCGATC GGTGCCCGTG ACGGCTCCCG GGACCCGAGA 
GAGAGGCTGG CGAAAAGCCA TGATGCAGAT TTACCTGGAA ATTCCGGAGC CCCTGAGAAC 
TTGACATTCA AAGAGCGCCA GCGGCTGTTC TCACAAGGTC AAGATGTGTC CAATAAAGTG 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110346.1
CDS	47..5557
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254578.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254578.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGC AGACTGGTCT GCCAGCAATC TCTGTTCTAG ATTTGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGT CAGGACAAGC TCTCCCACAC CGGACCAAAC 
TCTTCCGTGG GATCTTCCGG GGCAGCGATC GGTGCCCGTG ACGGCTCCCG GGACCCGAGA 
GAGAGGCTGG CGAAAAGCCA TGATGCAGAT TTACCTGGAA ATTCCGGAGC CCCTGAGAAC 
TTGACATTCA AAGAGCGCCA GCGGCTGTTC TCACAAGGTC AAGATGTGTC CAATAAAGTG 
AAAGCCTCTC GTAAATTAAC AGAACTGGAA AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254579.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110347.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OFb76138
Clone ID Related Accession (Same CDS sequence)	XM_023254579.1
Accession Version	XM_023254579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
GAAGTAGACC AGGTTGATCA GAAACAGAAG AATATCGCAG GGGCACTTGC TTTCTGGATG 
GCCAATGCAT CTGAGCTTCT CAACTTCATT AAGCAGGACC GAGATCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTTT AGCACACTTG GTTCAAATGG CGTTTAAATA TCTGGTTCAT 
TGTCTTCAGT CAGAACTGAA TAACTACATG CCAGCTTTTC TGGATGACCC TGAAGAGAAC 
AGTCTACAAA GACCAAAAAT AGATGACGTC TTGCACACGC TCACGGGAGC GATGGCGCTG 
CTGAGGCGCT GCAGGGTCAA TGCTGCTCTG ACCATCCAGC TTTTCTCTCA GCTCTTCCAC 
TTCATCAACA TGTGGCTCTT CAACAGATTG GTGACCGACC CCGAGTCGGG ACTGTGCTCT 
CACTACTGGG GGGCAATCAT CCGCCAGCAG CTGGGGCACG TGGAAGCCTG GGCAGAGAAG 
CAGGGATTAG AGCTGGCAGC CGACTGTCAC CTGAGCCGGA TAGTGCAGGC GACCACTTTG 
CTCACCATGG ACAAATACGC TCCTGAGGAC ACCCCAAATA TCAACAGCAC CTGCTTTAAG 
TTAAATTCGC TGCAGCTGCA GGCCTTGCTA CAGAATTACC ACTGTGCGCC CGACGAGCCC 
TTTGTCCCCA CGGACCTGAT AGAGAATGTC GTGGCCGTGG CCGAAAACAC GGCCGACGAG 
CTGGCACGCA GTGATGGGCG GGAGGTGCAG CTGGAAGAGG ACCCTGATCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG CTATTCTTGT GACGTCGTCA GGAACGTTCC AAACGGTTTA 
CAAGAATTTC TGGACCCTCT GTGCCAGAGA GGGTTTTGCA GGTTAATTCC TCATGCACGT 
TCACCAGGTA CTTGGACGAT ATATTTTGAA GGTGCAGATT ATGAGAGCCA TCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGCGG AAAGAACCTG AAATAATCAC TGTGACTCTC 
AAAAAGCAAA ACGGAATGGG CCTCAGCATT GTCGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTTGGAATCT ATGTCAAGTC TGTCGTAAAA GGAGGTGCTG CAGATGTGGA TGGACGCCTA 
GCCGCAGGTG ACCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTAGGACT CTCTCAAGAA 
AGGGCAGCAG AACTCATGAC AAGGACCAGT TCCGTGGTGA CACTGGAAGT AGCCAAGCAA 
GGAGCGATCT ACCACGGCCT GGCCACCCTG CTCAACCAGC CGTCCCCGAT GATGCAGAGA 
GTTTCAGACC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GCGAAGGTTT CGAGCTCTAT 
AACAATTCAG CTCAAAACGG GTCACCCGAG AGCCCTCAGC TGCCTTGGGC AGAATACAGC 
GAACCGAAGA AGCTCCCTGG CGATGACAGG CTGATGAAGA ACCGGGCTGA CCACCGCTCC 
AGCCCCAACG TGGCAAATCA GCCCCCCAGT CCCGGAGGGA AAAGCGCGTA CGCCCCTGGA 
GCAGCCGCTA AGATCACGTC CGTCTCCACC GGAAACCTCT GCACTGAGGA GCAGACCCCT 
CCGCCTCGAC CTGAGGCCTA CCCCATCCCC ACCCAGACGT ACACCAGGGA GTACTTCACC 
TTCCCGGCCT CCAGGTCCCA GGACCGCGTG GCTCCCCCTC AGAACCAGTG GCCCAATTAC 
GAGGAGAAGC CGCACGTGCA CACAGATGGT CATCAGTCCG GCCCCGCGAT GCAGCGCGTC 
ACCCGCTCCC AGGAGGAACT TCGGGAGGAT AAAGCTTACC AACTCGAGCG GCATCGGCTG 
GAGGCGGTGC TGGATCGCAA GTCCGACAGT GACGTGTGGA TCCAGCCGAG CTCCTCGGTG 
GGCTCCAGCA CGTCCAGCCA GGAGCACCTG GACCCCTGCT CCAAGTCGGG CACCCCCGCC 
TCCACGCTGA CCAAGAGCGG CCCCGGGCGC TGGAAGACGC CAGCCTCCGC GCAGCCCCTG 
CCGGCGGCCC CCCCGCCCGC GCAGACAGAC CTGCCCCCGC CACCCCCGCC GCCCCCCGCG 
CACTACGCCG GAGACTTCGA CGGGATCCCC GTGGACCTGC CCCTCCCGCC CCCTCCCAGC 
GGCCAGGCGG CGGCGGAGCG GAGGAAACGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCCCGCC TGGAGGAGGA GCGGGACAGG AAGCGCCGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGGCCCT GAACCCGGCC GCGTTCTCCC CAGTGACCGT CGCGCACACG 
AAGCCGGAAA AACCTTCCAC GCTCCAGAGG CCCCAGGACA CGGTCATCCG GGAGCTGCAG 
CCCCAGCAGC AGCCACGCAC CATCGAGCGC CGAGACTTGC AGTACATCAC GGTCAGCAAA 
GAGGAGCTGT CCTCGGGGGA CAGCCTGTCC CCCGACCCCT GGAAGCGGGA CGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA AATGCACATC GTGGACATGC TGAGCAGGGA GATCCGGGAG 
CTCCAGAGCA AGCCCGAGCG CAGCGCCGAG GACAGCGACC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGAGGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTCGAGGC TGAGCGGAGA 
GCAAGGCAGA CTGGTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGTCAG GACAAGCTCT CCCACACCGG ACCAAACTCT 
TCCGTGGGAT CTTCCGGGGC AGCGATCGGT GCCCGTGACG GCTCCCGGGA CCCGAGAGAG 
AGGCTGGCGA AAAGCCATGA TGCAGATTTA CCTGGAAATT CCGGAGCCCC TGAGAACTTG 
ACATTCAAAG AGCGCCAGCG GCTGTTCTCA CAAGGTCAAG ATGTGTCCAA TAAAGTGAAA 
GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110347.1
CDS	47..5554
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254579.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254579.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
GAAGTAGACC AGGTTGATCA GAAACAGAAG AATATCGCAG GGGCACTTGC TTTCTGGATG 
GCCAATGCAT CTGAGCTTCT CAACTTCATT AAGCAGGACC GAGATCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTTT AGCACACTTG GTTCAAATGG CGTTTAAATA TCTGGTTCAT 
TGTCTTCAGT CAGAACTGAA TAACTACATG CCAGCTTTTC TGGATGACCC TGAAGAGAAC 
AGTCTACAAA GACCAAAAAT AGATGACGTC TTGCACACGC TCACGGGAGC GATGGCGCTG 
CTGAGGCGCT GCAGGGTCAA TGCTGCTCTG ACCATCCAGC TTTTCTCTCA GCTCTTCCAC 
TTCATCAACA TGTGGCTCTT CAACAGATTG GTGACCGACC CCGAGTCGGG ACTGTGCTCT 
CACTACTGGG GGGCAATCAT CCGCCAGCAG CTGGGGCACG TGGAAGCCTG GGCAGAGAAG 
CAGGGATTAG AGCTGGCAGC CGACTGTCAC CTGAGCCGGA TAGTGCAGGC GACCACTTTG 
CTCACCATGG ACAAATACGC TCCTGAGGAC ACCCCAAATA TCAACAGCAC CTGCTTTAAG 
TTAAATTCGC TGCAGCTGCA GGCCTTGCTA CAGAATTACC ACTGTGCGCC CGACGAGCCC 
TTTGTCCCCA CGGACCTGAT AGAGAATGTC GTGGCCGTGG CCGAAAACAC GGCCGACGAG 
CTGGCACGCA GTGATGGGCG GGAGGTGCAG CTGGAAGAGG ACCCTGATCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG CTATTCTTGT GACGTCGTCA GGAACGTTCC AAACGGTTTA 
CAAGAATTTC TGGACCCTCT GTGCCAGAGA GGGTTTTGCA GGTTAATTCC TCATGCACGT 
TCACCAGGTA CTTGGACGAT ATATTTTGAA GGTGCAGATT ATGAGAGCCA TCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGCGG AAAGAACCTG AAATAATCAC TGTGACTCTC 
AAAAAGCAAA ACGGAATGGG CCTCAGCATT GTCGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTTGGAATCT ATGTCAAGTC TGTCGTAAAA GGAGGTGCTG CAGATGTGGA TGGACGCCTA 
GCCGCAGGTG ACCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTAGGACT CTCTCAAGAA 
AGGGCAGCAG AACTCATGAC AAGGACCAGT TCCGTGGTGA CACTGGAAGT AGCCAAGCAA 
GGAGCGATCT ACCACGGCCT GGCCACCCTG CTCAACCAGC CGTCCCCGAT GATGCAGAGA 
GTTTCAGACC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GCGAAGGTTT CGAGCTCTAT 
AACAATTCAG CTCAAAACGG GTCACCCGAG AGCCCTCAGC TGCCTTGGGC AGAATACAGC 
GAACCGAAGA AGCTCCCTGG CGATGACAGG CTGATGAAGA ACCGGGCTGA CCACCGCTCC 
AGCCCCAACG TGGCAAATCA GCCCCCCAGT CCCGGAGGGA AAAGCGCGTA CGCCCCTGGA 
GCAGCCGCTA AGATCACGTC CGTCTCCACC GGAAACCTCT GCACTGAGGA GCAGACCCCT 
CCGCCTCGAC CTGAGGCCTA CCCCATCCCC ACCCAGACGT ACACCAGGGA GTACTTCACC 
TTCCCGGCCT CCAGGTCCCA GGACCGCGTG GCTCCCCCTC AGAACCAGTG GCCCAATTAC 
GAGGAGAAGC CGCACGTGCA CACAGATGGT CATCAGTCCG GCCCCGCGAT GCAGCGCGTC 
ACCCGCTCCC AGGAGGAACT TCGGGAGGAT AAAGCTTACC AACTCGAGCG GCATCGGCTG 
GAGGCGGTGC TGGATCGCAA GTCCGACAGT GACGTGTGGA TCCAGCCGAG CTCCTCGGTG 
GGCTCCAGCA CGTCCAGCCA GGAGCACCTG GACCCCTGCT CCAAGTCGGG CACCCCCGCC 
TCCACGCTGA CCAAGAGCGG CCCCGGGCGC TGGAAGACGC CAGCCTCCGC GCAGCCCCTG 
CCGGCGGCCC CCCCGCCCGC GCAGACAGAC CTGCCCCCGC CACCCCCGCC GCCCCCCGCG 
CACTACGCCG GAGACTTCGA CGGGATCCCC GTGGACCTGC CCCTCCCGCC CCCTCCCAGC 
GGCCAGGCGG CGGCGGAGCG GAGGAAACGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCCCGCC TGGAGGAGGA GCGGGACAGG AAGCGCCGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGGCCCT GAACCCGGCC GCGTTCTCCC CAGTGACCGT CGCGCACACG 
AAGCCGGAAA AACCTTCCAC GCTCCAGAGG CCCCAGGACA CGGTCATCCG GGAGCTGCAG 
CCCCAGCAGC AGCCACGCAC CATCGAGCGC CGAGACTTGC AGTACATCAC GGTCAGCAAA 
GAGGAGCTGT CCTCGGGGGA CAGCCTGTCC CCCGACCCCT GGAAGCGGGA CGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA AATGCACATC GTGGACATGC TGAGCAGGGA GATCCGGGAG 
CTCCAGAGCA AGCCCGAGCG CAGCGCCGAG GACAGCGACC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGAGGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTCGAGGC TGAGCGGAGA 
GCAAGGCAGA CTGGTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGTCAG GACAAGCTCT CCCACACCGG ACCAAACTCT 
TCCGTGGGAT CTTCCGGGGC AGCGATCGGT GCCCGTGACG GCTCCCGGGA CCCGAGAGAG 
AGGCTGGCGA AAAGCCATGA TGCAGATTTA CCTGGAAATT CCGGAGCCCC TGAGAACTTG 
ACATTCAAAG AGCGCCAGCG GCTGTTCTCA CAAGGTCAAG ATGTGTCCAA TAAAGTGAAA 
GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254580.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110348.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OFb76139
Clone ID Related Accession (Same CDS sequence)	XM_023254580.1
Accession Version	XM_023254580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
GAAGTAGACC AGGTTGATCA GAAACAGAAG AATATCGCAG GGGCACTTGC TTTCTGGATG 
GCCAATGCAT CTGAGCTTCT CAACTTCATT AAGCAGGACC GAGATCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTTT AGCACACTTG GTTCAAATGG CGTTTAAATA TCTGGTTCAT 
TGTCTTCAGT CAGAACTGAA TAACTACATG CCAGCTTTTC TGGATGACCC TGAAGAGAAC 
AGTCTACAAA GACCAAAAAT AGATGACGTC TTGCACACGC TCACGGGAGC GATGGCGCTG 
CTGAGGCGCT GCAGGGTCAA TGCTGCTCTG ACCATCCAGC TTTTCTCTCA GCTCTTCCAC 
TTCATCAACA TGTGGCTCTT CAACAGATTG GTGACCGACC CCGAGTCGGG ACTGTGCTCT 
CACTACTGGG GGGCAATCAT CCGCCAGCAG CTGGGGCACG TGGAAGCCTG GGCAGAGAAG 
CAGGGATTAG AGCTGGCAGC CGACTGTCAC CTGAGCCGGA TAGTGCAGGC GACCACTTTG 
CTCACCATGG ACAAATACGC TCCTGAGGAC ACCCCAAATA TCAACAGCAC CTGCTTTAAG 
TTAAATTCGC TGCAGCTGCA GGCCTTGCTA CAGAATTACC ACTGTGCGCC CGACGAGCCC 
TTTGTCCCCA CGGACCTGAT AGAGAATGTC GTGGCCGTGG CCGAAAACAC GGCCGACGAG 
CTGGCACGCA GTGATGGGCG GGAGGTGCAG CTGGAAGAGG ACCCTGATCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG CTATTCTTGT GACGTCGTCA GGAACGTTCC AAACGGTTTA 
CAAGAATTTC TGGACCCTCT GTGCCAGAGA GGGTTTTGCA GGTTAATTCC TCATGCACGT 
TCACCAGGTA CTTGGACGAT ATATTTTGAA GGTGCAGATT ATGAGAGCCA TCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGCGG AAAGAACCTG AAATAATCAC TGTGACTCTC 
AAAAAGCAAA ACGGAATGGG CCTCAGCATT GTCGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTTGGAATCT ATGTCAAGTC TGTCGTAAAA GGAGGTGCTG CAGATGTGGA TGGACGCCTA 
GCCGCAGGTG ACCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTAGGACT CTCTCAAGAA 
AGGGCAGCAG AACTCATGAC AAGGACCAGT TCCGTGGTGA CACTGGAAGT AGCCAAGCAA 
GGAGCGATCT ACCACGGCCT GGCCACCCTG CTCAACCAGC CGTCCCCGAT GATGCAGAGA 
GTTTCAGACC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GCGAAGGTTT CGAGCTCTAT 
AACAATTCAG CTCAAAACGG GTCACCCGAG AGCCCTCAGC TGCCTTGGGC AGAATACAGC 
GAACCGAAGA AGCTCCCTGG CGATGACAGG CTGATGAAGA ACCGGGCTGA CCACCGCTCC 
AGCCCCAACG TGGCAAATCA GCCCCCCAGT CCCGGAGGGA AAAGCGCGTA CGCCCCTGGA 
GCAGCCGCTA AGATCACGTC CGTCTCCACC GGAAACCTCT GCACTGAGGA GCAGACCCCT 
CCGCCTCGAC CTGAGGCCTA CCCCATCCCC ACCCAGACGT ACACCAGGGA GTACTTCACC 
TTCCCGGCCT CCAGGTCCCA GGACCGCGTG GCTCCCCCTC AGAACCAGTG GCCCAATTAC 
GAGGAGAAGC CGCACGTGCA CACAGATGGT CATCAGTCCG GCCCCGCGAT GCAGCGCGTC 
ACCCGCTCCC AGGAGGAACT TCGGGAGGAT AAAGCTTACC AACTCGAGCG GCATCGGCTG 
GAGGCGGTGC TGGATCGCAA GTCCGACAGT GACGTGTGGA TCCAGCCGAG CTCCTCGGTG 
GGCTCCAGCA CGTCCAGCCA GGAGCACCTG GACCCCTGCT CCAAGTCGGG CACCCCCGCC 
TCCACGCTGA CCAAGAGCGG CCCCGGGCGC TGGAAGACGC CAGCCTCCGC GCAGCCCCTG 
CCGGCGGCCC CCCCGCCCGC GCAGACAGAC CTGCCCCCGC CACCCCCGCC GCCCCCCGCG 
CACTACGCCG GAGACTTCGA CGGGATCCCC GTGGACCTGC CCCTCCCGCC CCCTCCCAGC 
GGCCAGGCGG CGGCGGAGCG GAGGAAACGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCCCGCC TGGAGGAGGA GCGGGACAGG AAGCGCCGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGGCCCT GAACCCGGCC GCGTTCTCCC CAGTGACCGT CGCGCACACG 
AAGCCGGAAA AACCTTCCAC GCTCCAGAGG CCCCAGGACA CGGTCATCCG GGAGCTGCAG 
CCCCAGCAGC AGCCACGCAC CATCGAGCGC CGAGACTTGC AGTACATCAC GGTCAGCAAA 
GAGGAGCTGT CCTCGGGGGA CAGCCTGTCC CCCGACCCCT GGAAGCGGGA CGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA AATGCACATC GTGGACATGC TGAGCAGGGA GATCCGGGAG 
CTCCAGAGCA AGCCCGAGCG CAGCGCCGAG GACAGCGACC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGAGGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTCGAGGC TGAGCGGAGA 
GCAAGGCAGA CTGGTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGTCAG GACAAGCTCT CCCACACCGG ACCAAACTCT 
TCCGTGGGAT CTTCCGGGGC AGCGATCGGT GCCCGTGACG GCTCCCGGGA CCCGAGAGAG 
AGGCTGGCGA AAAGCCATGA TGCAGATTTA CCTGGAAATT CCGGAGCCCC TGAGAACTTG 
ACATTCAAAG AGCGCCAGCG GCTGTTCTCA CAAGGTCAAG ATGTGTCCAA TAAAGTGAAA 
GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110348.1
CDS	47..5554
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254580.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254580.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
GAAGTAGACC AGGTTGATCA GAAACAGAAG AATATCGCAG GGGCACTTGC TTTCTGGATG 
GCCAATGCAT CTGAGCTTCT CAACTTCATT AAGCAGGACC GAGATCTTAG TCGAATCACA 
TTGGATGCCC AAGATGTTTT AGCACACTTG GTTCAAATGG CGTTTAAATA TCTGGTTCAT 
TGTCTTCAGT CAGAACTGAA TAACTACATG CCAGCTTTTC TGGATGACCC TGAAGAGAAC 
AGTCTACAAA GACCAAAAAT AGATGACGTC TTGCACACGC TCACGGGAGC GATGGCGCTG 
CTGAGGCGCT GCAGGGTCAA TGCTGCTCTG ACCATCCAGC TTTTCTCTCA GCTCTTCCAC 
TTCATCAACA TGTGGCTCTT CAACAGATTG GTGACCGACC CCGAGTCGGG ACTGTGCTCT 
CACTACTGGG GGGCAATCAT CCGCCAGCAG CTGGGGCACG TGGAAGCCTG GGCAGAGAAG 
CAGGGATTAG AGCTGGCAGC CGACTGTCAC CTGAGCCGGA TAGTGCAGGC GACCACTTTG 
CTCACCATGG ACAAATACGC TCCTGAGGAC ACCCCAAATA TCAACAGCAC CTGCTTTAAG 
TTAAATTCGC TGCAGCTGCA GGCCTTGCTA CAGAATTACC ACTGTGCGCC CGACGAGCCC 
TTTGTCCCCA CGGACCTGAT AGAGAATGTC GTGGCCGTGG CCGAAAACAC GGCCGACGAG 
CTGGCACGCA GTGATGGGCG GGAGGTGCAG CTGGAAGAGG ACCCTGATCT GCAGCTGCCT 
TTTCTTTTGC CGGAAGACGG CTATTCTTGT GACGTCGTCA GGAACGTTCC AAACGGTTTA 
CAAGAATTTC TGGACCCTCT GTGCCAGAGA GGGTTTTGCA GGTTAATTCC TCATGCACGT 
TCACCAGGTA CTTGGACGAT ATATTTTGAA GGTGCAGATT ATGAGAGCCA TCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGCGG AAAGAACCTG AAATAATCAC TGTGACTCTC 
AAAAAGCAAA ACGGAATGGG CCTCAGCATT GTCGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTTGGAATCT ATGTCAAGTC TGTCGTAAAA GGAGGTGCTG CAGATGTGGA TGGACGCCTA 
GCCGCAGGTG ACCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTAGGACT CTCTCAAGAA 
AGGGCAGCAG AACTCATGAC AAGGACCAGT TCCGTGGTGA CACTGGAAGT AGCCAAGCAA 
GGAGCGATCT ACCACGGCCT GGCCACCCTG CTCAACCAGC CGTCCCCGAT GATGCAGAGA 
GTTTCAGACC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GCGAAGGTTT CGAGCTCTAT 
AACAATTCAG CTCAAAACGG GTCACCCGAG AGCCCTCAGC TGCCTTGGGC AGAATACAGC 
GAACCGAAGA AGCTCCCTGG CGATGACAGG CTGATGAAGA ACCGGGCTGA CCACCGCTCC 
AGCCCCAACG TGGCAAATCA GCCCCCCAGT CCCGGAGGGA AAAGCGCGTA CGCCCCTGGA 
GCAGCCGCTA AGATCACGTC CGTCTCCACC GGAAACCTCT GCACTGAGGA GCAGACCCCT 
CCGCCTCGAC CTGAGGCCTA CCCCATCCCC ACCCAGACGT ACACCAGGGA GTACTTCACC 
TTCCCGGCCT CCAGGTCCCA GGACCGCGTG GCTCCCCCTC AGAACCAGTG GCCCAATTAC 
GAGGAGAAGC CGCACGTGCA CACAGATGGT CATCAGTCCG GCCCCGCGAT GCAGCGCGTC 
ACCCGCTCCC AGGAGGAACT TCGGGAGGAT AAAGCTTACC AACTCGAGCG GCATCGGCTG 
GAGGCGGTGC TGGATCGCAA GTCCGACAGT GACGTGTGGA TCCAGCCGAG CTCCTCGGTG 
GGCTCCAGCA CGTCCAGCCA GGAGCACCTG GACCCCTGCT CCAAGTCGGG CACCCCCGCC 
TCCACGCTGA CCAAGAGCGG CCCCGGGCGC TGGAAGACGC CAGCCTCCGC GCAGCCCCTG 
CCGGCGGCCC CCCCGCCCGC GCAGACAGAC CTGCCCCCGC CACCCCCGCC GCCCCCCGCG 
CACTACGCCG GAGACTTCGA CGGGATCCCC GTGGACCTGC CCCTCCCGCC CCCTCCCAGC 
GGCCAGGCGG CGGCGGAGCG GAGGAAACGG GAGGAGCAGC AGCGCTGGTA CGAGAAGGAG 
AAGGCCCGCC TGGAGGAGGA GCGGGACAGG AAGCGCCGGG AGCAGGAGCG GAAGCTGGGC 
CAGATGCGCT CCCAGGCCCT GAACCCGGCC GCGTTCTCCC CAGTGACCGT CGCGCACACG 
AAGCCGGAAA AACCTTCCAC GCTCCAGAGG CCCCAGGACA CGGTCATCCG GGAGCTGCAG 
CCCCAGCAGC AGCCACGCAC CATCGAGCGC CGAGACTTGC AGTACATCAC GGTCAGCAAA 
GAGGAGCTGT CCTCGGGGGA CAGCCTGTCC CCCGACCCCT GGAAGCGGGA CGCCAGGGAG 
AAGCTGGAGA AGCAGCAGCA AATGCACATC GTGGACATGC TGAGCAGGGA GATCCGGGAG 
CTCCAGAGCA AGCCCGAGCG CAGCGCCGAG GACAGCGACC GCCTGCGCAA GCTCATGCTG 
GAGTGGCAGT TCCAGAAGAG ACTGCAGGAG TCCAAGCAGA AGGACGAGGA CGACGAGGAG 
GAGGAGGACG ACGACGTGGA CACCATGCTG ATCATGCAGC GCCTCGAGGC TGAGCGGAGA 
GCAAGGCAGA CTGGTCTGCC AGCAATCTCT GTTCTAGATT TGTTACAGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGTCAG GACAAGCTCT CCCACACCGG ACCAAACTCT 
TCCGTGGGAT CTTCCGGGGC AGCGATCGGT GCCCGTGACG GCTCCCGGGA CCCGAGAGAG 
AGGCTGGCGA AAAGCCATGA TGCAGATTTA CCTGGAAATT CCGGAGCCCC TGAGAACTTG 
ACATTCAAAG AGCGCCAGCG GCTGTTCTCA CAAGGTCAAG ATGTGTCCAA TAAAGTGAAA 
GCCTCTCGTA AATTAACAGA ACTGGAAAAC GAACTGAACA CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254581.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110349.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

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CloneID	OFb76140
Clone ID Related Accession (Same CDS sequence)	XM_023254581.1
Accession Version	XM_023254581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGC AGACTGGTCT GCCAGCAATC TCTGTTCTAG ATTTGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGTCAGGAC AAGCTCTCCC ACACCGGACC AAACTCTTCC 
GTGGGATCTT CCGGGGCAGC GATCGGTGCC CGTGACGGCT CCCGGGACCC GAGAGAGAGG 
CTGGCGAAAA GCCATGATGC AGATTTACCT GGAAATTCCG GAGCCCCTGA GAACTTGACA 
TTCAAAGAGC GCCAGCGGCT GTTCTCACAA GGTCAAGATG TGTCCAATAA AGTGAAAGCC 
TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110349.1
CDS	47..5551
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254581.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254581.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGC AGACTGGTCT GCCAGCAATC TCTGTTCTAG ATTTGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGAGGCGAC AGGAAGAAGG TTATTACAGC 
CGCCTGGAAG CCGAGAGGCG CAGACAGCAC GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG 
GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC 
TCCCCGGCGG CTGCTCCTCC TCCCCCACCT CAGCGGACCG CCTCCTCCCT GCAGGCCCCG 
GCCCTGTCCC CAGACTCCCT GTACACCGCC AGGCTCGTTG CGTACCACGA GGAGGAGGAG 
GAGGAGGACT GCAGTCTGGC AGGTCAGGAC AAGCTCTCCC ACACCGGACC AAACTCTTCC 
GTGGGATCTT CCGGGGCAGC GATCGGTGCC CGTGACGGCT CCCGGGACCC GAGAGAGAGG 
CTGGCGAAAA GCCATGATGC AGATTTACCT GGAAATTCCG GAGCCCCTGA GAACTTGACA 
TTCAAAGAGC GCCAGCGGCT GTTCTCACAA GGTCAAGATG TGTCCAATAA AGTGAAAGCC 
TCTCGTAAAT TAACAGAACT GGAAAACGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254582.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110350.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

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CloneID	OFb76141
Clone ID Related Accession (Same CDS sequence)	XM_023254582.1
Accession Version	XM_023254582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGAAACAGA AGAATATCGC AGGGGCACTT GCTTTCTGGA TGGCCAATGC ATCTGAGCTT 
CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CATTGGATGC CCAAGATGTT 
TTAGCACACT TGGTTCAAAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA GTCAGAACTG 
AATAACTACA TGCCAGCTTT TCTGGATGAC CCTGAAGAGA ACAGTCTACA AAGACCAAAA 
ATAGATGACG TCTTGCACAC GCTCACGGGA GCGATGGCGC TGCTGAGGCG CTGCAGGGTC 
AATGCTGCTC TGACCATCCA GCTTTTCTCT CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAGAT TGGTGACCGA CCCCGAGTCG GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC TGGGCAGAGA AGCAGGGATT AGAGCTGGCA 
GCCGACTGTC ACCTGAGCCG GATAGTGCAG GCGACCACTT TGCTCACCAT GGACAAATAC 
GCTCCTGAGG ACACCCCAAA TATCAACAGC ACCTGCTTTA AGTTAAATTC GCTGCAGCTG 
CAGGCCTTGC TACAGAATTA CCACTGTGCG CCCGACGAGC CCTTTGTCCC CACGGACCTG 
ATAGAGAATG TCGTGGCCGT GGCCGAAAAC ACGGCCGACG AGCTGGCACG CAGTGATGGG 
CGGGAGGTGC AGCTGGAAGA GGACCCTGAT CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC 
GGCTATTCTT GTGACGTCGT CAGGAACGTT CCAAACGGTT TACAAGAATT TCTGGACCCT 
CTGTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCATGCAC GTTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CATCTTCTGC GTGAGAACAC AGAGCTGGCT 
CAGCCTCTGC GGAAAGAACC TGAAATAATC ACTGTGACTC TCAAAAAGCA AAACGGAATG 
GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG 
TCTGTCGTAA AAGGAGGTGC TGCAGATGTG GATGGACGCC TAGCCGCAGG TGACCAGCTC 
CTCAGTGTGG ATGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCAGC AGAACTCATG 
ACAAGGACCA GTTCCGTGGT GACACTGGAA GTAGCCAAGC AAGGAGCGAT CTACCACGGC 
CTGGCCACCC TGCTCAACCA GCCGTCCCCG ATGATGCAGA GAGTTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTCGAGCTCT ATAACAATTC AGCTCAAAAC 
GGGTCACCCG AGAGCCCTCA GCTGCCTTGG GCAGAATACA GCGAACCGAA GAAGCTCCCT 
GGCGATGACA GGCTGATGAA GAACCGGGCT GACCACCGCT CCAGCCCCAA CGTGGCAAAT 
CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG TACGCCCCTG GAGCAGCCGC TAAGATCACG 
TCCGTCTCCA CCGGAAACCT CTGCACTGAG GAGCAGACCC CTCCGCCTCG ACCTGAGGCC 
TACCCCATCC CCACCCAGAC GTACACCAGG GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGCTCCCCC TCAGAACCAG TGGCCCAATT ACGAGGAGAA GCCGCACGTG 
CACACAGATG GTCATCAGTC CGGCCCCGCG ATGCAGCGCG TCACCCGCTC CCAGGAGGAA 
CTTCGGGAGG ATAAAGCTTA CCAACTCGAG CGGCATCGGC TGGAGGCGGT GCTGGATCGC 
AAGTCCGACA GTGACGTGTG GATCCAGCCG AGCTCCTCGG TGGGCTCCAG CACGTCCAGC 
CAGGAGCACC TGGACCCCTG CTCCAAGTCG GGCACCCCCG CCTCCACGCT GACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC 
GCGCAGACAG ACCTGCCCCC GCCACCCCCG CCGCCCCCCG CGCACTACGC CGGAGACTTC 
GACGGGATCC CCGTGGACCT GCCCCTCCCG CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG 
CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGACA GGAAGCGCCG GGAGCAGGAG CGGAAGCTGG GCCAGATGCG CTCCCAGGCC 
CTGAACCCGG CCGCGTTCTC CCCAGTGACC GTCGCGCACA CGAAGCCGGA AAAACCTTCC 
ACGCTCCAGA GGCCCCAGGA CACGGTCATC CGGGAGCTGC AGCCCCAGCA GCAGCCACGC 
ACCATCGAGC GCCGAGACTT GCAGTACATC ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG 
GACAGCCTGT CCCCCGACCC CTGGAAGCGG GACGCCAGGG AGAAGCTGGA GAAGCAGCAG 
CAAATGCACA TCGTGGACAT GCTGAGCAGG GAGATCCGGG AGCTCCAGAG CAAGCCCGAG 
CGCAGCGCCG AGGACAGCGA CCGCCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAAGGACGAG GACGACGAGG AGGAGGAGGA CGACGACGTG 
GACACCATGC TGATCATGCA GCGCCTCGAG GCTGAGCGGA GAGCAAGGCA GACTGGTCTG 
CCAGCAATCT CTGTTCTAGA TTTGTTACAG GACGAGGAGC GGCGGCGGCA GCGGCAGCTG 
GACGACATGC GCAGGCGGGA AGCGGAGGAC CGCGCCAGGC AGGAGGAGGA GAGGACGAAG 
CGAGACGCCG AAGACAAGAG GCGACAGGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGCTGCTGG AGCCGGAGGA GCCGGGCCTG 
GGCAGACCGC CGCTGCCGCG GGGCTACGAG CTCCCGGCCC CGTCCTCCCC GGCGGCTGCT 
CCTCCTCCCC CACCTCAGCG GACCGCCTCC TCCCTGCAGG CCCCGGCCCT GTCCCCAGAC 
TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GGACTGCAGT 
CTGGCAGGTC AGGACAAGCT CTCCCACACC GGACCAAACT CTTCCGTGGG ATCTTCCGGG 
GCAGCGATCG GTGCCCGTGA CGGCTCCCGG GACCCGAGAG AGAGGCTGGC GAAAAGCCAT 
GATGCAGATT TACCTGGAAA TTCCGGAGCC CCTGAGAACT TGACATTCAA AGAGCGCCAG 
CGGCTGTTCT CACAAGGTCA AGATGTGTCC AATAAAGTGA AAGCCTCTCG TAAATTAACA 
GAACTGGAAA ACGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110350.1
CDS	47..5536
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254582.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254582.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGAAACAGA AGAATATCGC AGGGGCACTT GCTTTCTGGA TGGCCAATGC ATCTGAGCTT 
CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CATTGGATGC CCAAGATGTT 
TTAGCACACT TGGTTCAAAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA GTCAGAACTG 
AATAACTACA TGCCAGCTTT TCTGGATGAC CCTGAAGAGA ACAGTCTACA AAGACCAAAA 
ATAGATGACG TCTTGCACAC GCTCACGGGA GCGATGGCGC TGCTGAGGCG CTGCAGGGTC 
AATGCTGCTC TGACCATCCA GCTTTTCTCT CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAGAT TGGTGACCGA CCCCGAGTCG GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC TGGGCAGAGA AGCAGGGATT AGAGCTGGCA 
GCCGACTGTC ACCTGAGCCG GATAGTGCAG GCGACCACTT TGCTCACCAT GGACAAATAC 
GCTCCTGAGG ACACCCCAAA TATCAACAGC ACCTGCTTTA AGTTAAATTC GCTGCAGCTG 
CAGGCCTTGC TACAGAATTA CCACTGTGCG CCCGACGAGC CCTTTGTCCC CACGGACCTG 
ATAGAGAATG TCGTGGCCGT GGCCGAAAAC ACGGCCGACG AGCTGGCACG CAGTGATGGG 
CGGGAGGTGC AGCTGGAAGA GGACCCTGAT CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC 
GGCTATTCTT GTGACGTCGT CAGGAACGTT CCAAACGGTT TACAAGAATT TCTGGACCCT 
CTGTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCATGCAC GTTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CATCTTCTGC GTGAGAACAC AGAGCTGGCT 
CAGCCTCTGC GGAAAGAACC TGAAATAATC ACTGTGACTC TCAAAAAGCA AAACGGAATG 
GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG 
TCTGTCGTAA AAGGAGGTGC TGCAGATGTG GATGGACGCC TAGCCGCAGG TGACCAGCTC 
CTCAGTGTGG ATGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCAGC AGAACTCATG 
ACAAGGACCA GTTCCGTGGT GACACTGGAA GTAGCCAAGC AAGGAGCGAT CTACCACGGC 
CTGGCCACCC TGCTCAACCA GCCGTCCCCG ATGATGCAGA GAGTTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTCGAGCTCT ATAACAATTC AGCTCAAAAC 
GGGTCACCCG AGAGCCCTCA GCTGCCTTGG GCAGAATACA GCGAACCGAA GAAGCTCCCT 
GGCGATGACA GGCTGATGAA GAACCGGGCT GACCACCGCT CCAGCCCCAA CGTGGCAAAT 
CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG TACGCCCCTG GAGCAGCCGC TAAGATCACG 
TCCGTCTCCA CCGGAAACCT CTGCACTGAG GAGCAGACCC CTCCGCCTCG ACCTGAGGCC 
TACCCCATCC CCACCCAGAC GTACACCAGG GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGCTCCCCC TCAGAACCAG TGGCCCAATT ACGAGGAGAA GCCGCACGTG 
CACACAGATG GTCATCAGTC CGGCCCCGCG ATGCAGCGCG TCACCCGCTC CCAGGAGGAA 
CTTCGGGAGG ATAAAGCTTA CCAACTCGAG CGGCATCGGC TGGAGGCGGT GCTGGATCGC 
AAGTCCGACA GTGACGTGTG GATCCAGCCG AGCTCCTCGG TGGGCTCCAG CACGTCCAGC 
CAGGAGCACC TGGACCCCTG CTCCAAGTCG GGCACCCCCG CCTCCACGCT GACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC 
GCGCAGACAG ACCTGCCCCC GCCACCCCCG CCGCCCCCCG CGCACTACGC CGGAGACTTC 
GACGGGATCC CCGTGGACCT GCCCCTCCCG CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG 
CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGACA GGAAGCGCCG GGAGCAGGAG CGGAAGCTGG GCCAGATGCG CTCCCAGGCC 
CTGAACCCGG CCGCGTTCTC CCCAGTGACC GTCGCGCACA CGAAGCCGGA AAAACCTTCC 
ACGCTCCAGA GGCCCCAGGA CACGGTCATC CGGGAGCTGC AGCCCCAGCA GCAGCCACGC 
ACCATCGAGC GCCGAGACTT GCAGTACATC ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG 
GACAGCCTGT CCCCCGACCC CTGGAAGCGG GACGCCAGGG AGAAGCTGGA GAAGCAGCAG 
CAAATGCACA TCGTGGACAT GCTGAGCAGG GAGATCCGGG AGCTCCAGAG CAAGCCCGAG 
CGCAGCGCCG AGGACAGCGA CCGCCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAAGGACGAG GACGACGAGG AGGAGGAGGA CGACGACGTG 
GACACCATGC TGATCATGCA GCGCCTCGAG GCTGAGCGGA GAGCAAGGCA GACTGGTCTG 
CCAGCAATCT CTGTTCTAGA TTTGTTACAG GACGAGGAGC GGCGGCGGCA GCGGCAGCTG 
GACGACATGC GCAGGCGGGA AGCGGAGGAC CGCGCCAGGC AGGAGGAGGA GAGGACGAAG 
CGAGACGCCG AAGACAAGAG GCGACAGGAA GAAGGTTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGCGGA GGCCGAGCGC AGGCTGCTGG AGCCGGAGGA GCCGGGCCTG 
GGCAGACCGC CGCTGCCGCG GGGCTACGAG CTCCCGGCCC CGTCCTCCCC GGCGGCTGCT 
CCTCCTCCCC CACCTCAGCG GACCGCCTCC TCCCTGCAGG CCCCGGCCCT GTCCCCAGAC 
TCCCTGTACA CCGCCAGGCT CGTTGCGTAC CACGAGGAGG AGGAGGAGGA GGACTGCAGT 
CTGGCAGGTC AGGACAAGCT CTCCCACACC GGACCAAACT CTTCCGTGGG ATCTTCCGGG 
GCAGCGATCG GTGCCCGTGA CGGCTCCCGG GACCCGAGAG AGAGGCTGGC GAAAAGCCAT 
GATGCAGATT TACCTGGAAA TTCCGGAGCC CCTGAGAACT TGACATTCAA AGAGCGCCAG 
CGGCTGTTCT CACAAGGTCA AGATGTGTCC AATAAAGTGA AAGCCTCTCG TAAATTAACA 
GAACTGGAAA ACGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254583.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110351.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

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CloneID	OFb76142
Clone ID Related Accession (Same CDS sequence)	XM_023254583.1
Accession Version	XM_023254583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGC AGACTGGTCT GCCAGCAATC TCTGTTCTAG ATTTGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGA CCAAACTCTT CCGTGGGATC TTCCGGGGCA 
GCGATCGGTG CCCGTGACGG CTCCCGGGAC CCGAGAGAGA GGCTGGCGAA AAGCCATGAT 
GCAGATTTAC CTGGAAATTC CGGAGCCCCT GAGAACTTGA CATTCAAAGA GCGCCAGCGG 
CTGTTCTCAC AAGGTCAAGA TGTGTCCAAT AAAGTGAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110351.1
CDS	46..5532
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254583.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254583.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGC AGACTGGTCT GCCAGCAATC TCTGTTCTAG ATTTGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGA CCAAACTCTT CCGTGGGATC TTCCGGGGCA 
GCGATCGGTG CCCGTGACGG CTCCCGGGAC CCGAGAGAGA GGCTGGCGAA AAGCCATGAT 
GCAGATTTAC CTGGAAATTC CGGAGCCCCT GAGAACTTGA CATTCAAAGA GCGCCAGCGG 
CTGTTCTCAC AAGGTCAAGA TGTGTCCAAT AAAGTGAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254584.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110352.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OFb76143
Clone ID Related Accession (Same CDS sequence)	XM_023254584.1
Accession Version	XM_023254584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGT TACAGGACGA GGAGCGGCGG CGGCAGCGGC AGCTGGACGA CATGCGCAGG 
CGGGAAGCGG AGGACCGCGC CAGGCAGGAG GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC 
AAGAGGCGAC AGGAAGAAGG TTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG 
CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC TCCCCGGCGG CTGCTCCTCC TCCCCCACCT 
CAGCGGACCG CCTCCTCCCT GCAGGCCCCG GCCCTGTCCC CAGACTCCCT GTACACCGCC 
AGGCTCGTTG CGTACCACGA GGAGGAGGAG GAGGAGGACT GCAGTCTGGC AGGTCAGGAC 
AAGCTCTCCC ACACCGGACC AAACTCTTCC GTGGGATCTT CCGGGGCAGC GATCGGTGCC 
CGTGACGGCT CCCGGGACCC GAGAGAGAGG CTGGCGAAAA GCCATGATGC AGATTTACCT 
GGAAATTCCG GAGCCCCTGA GAACTTGACA TTCAAAGAGC GCCAGCGGCT GTTCTCACAA 
GGTCAAGATG TGTCCAATAA AGTGAAAGCC TCTCGTAAAT TAACAGAACT GGAAAACGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110352.1
CDS	46..5520
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254584.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254584.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGT TACAGGACGA GGAGCGGCGG CGGCAGCGGC AGCTGGACGA CATGCGCAGG 
CGGGAAGCGG AGGACCGCGC CAGGCAGGAG GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC 
AAGAGGCGAC AGGAAGAAGG TTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GCGGAGGCCG AGCGCAGGCT GCTGGAGCCG GAGGAGCCGG GCCTGGGCAG ACCGCCGCTG 
CCGCGGGGCT ACGAGCTCCC GGCCCCGTCC TCCCCGGCGG CTGCTCCTCC TCCCCCACCT 
CAGCGGACCG CCTCCTCCCT GCAGGCCCCG GCCCTGTCCC CAGACTCCCT GTACACCGCC 
AGGCTCGTTG CGTACCACGA GGAGGAGGAG GAGGAGGACT GCAGTCTGGC AGGTCAGGAC 
AAGCTCTCCC ACACCGGACC AAACTCTTCC GTGGGATCTT CCGGGGCAGC GATCGGTGCC 
CGTGACGGCT CCCGGGACCC GAGAGAGAGG CTGGCGAAAA GCCATGATGC AGATTTACCT 
GGAAATTCCG GAGCCCCTGA GAACTTGACA TTCAAAGAGC GCCAGCGGCT GTTCTCACAA 
GGTCAAGATG TGTCCAATAA AGTGAAAGCC TCTCGTAAAT TAACAGAACT GGAAAACGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254585.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110353.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

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CloneID	OFb76144
Clone ID Related Accession (Same CDS sequence)	XM_023254585.1
Accession Version	XM_023254585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGG ACGAGGAGCG GCGGCGGCAG CGGCAGCTGG ACGACATGCG CAGGCGGGAA 
GCGGAGGACC GCGCCAGGCA GGAGGAGGAG AGGACGAAGC GAGACGCCGA AGACAAGAGG 
CGACAGGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG CCGGGCCTGG GCAGACCGCC GCTGCCGCGG 
GGCTACGAGC TCCCGGCCCC GTCCTCCCCG GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG 
ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG TCCCCAGACT CCCTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGA GGAGGAGGAG GACTGCAGTC TGGCAGGTCA GGACAAGCTC 
TCCCACACCG GACCAAACTC TTCCGTGGGA TCTTCCGGGG CAGCGATCGG TGCCCGTGAC 
GGCTCCCGGG ACCCGAGAGA GAGGCTGGCG AAAAGCCATG ATGCAGATTT ACCTGGAAAT 
TCCGGAGCCC CTGAGAACTT GACATTCAAA GAGCGCCAGC GGCTGTTCTC ACAAGGTCAA 
GATGTGTCCA ATAAAGTGAA AGCCTCTCGT AAATTAACAG AACTGGAAAA CGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110353.1
CDS	47..5515
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254585.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254585.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGGAAGTAG ACCAGGTTGA TCAGAAACAG AAGAATATCG CAGGGGCACT TGCTTTCTGG 
ATGGCCAATG CATCTGAGCT TCTCAACTTC ATTAAGCAGG ACCGAGATCT TAGTCGAATC 
ACATTGGATG CCCAAGATGT TTTAGCACAC TTGGTTCAAA TGGCGTTTAA ATATCTGGTT 
CATTGTCTTC AGTCAGAACT GAATAACTAC ATGCCAGCTT TTCTGGATGA CCCTGAAGAG 
AACAGTCTAC AAAGACCAAA AATAGATGAC GTCTTGCACA CGCTCACGGG AGCGATGGCG 
CTGCTGAGGC GCTGCAGGGT CAATGCTGCT CTGACCATCC AGCTTTTCTC TCAGCTCTTC 
CACTTCATCA ACATGTGGCT CTTCAACAGA TTGGTGACCG ACCCCGAGTC GGGACTGTGC 
TCTCACTACT GGGGGGCAAT CATCCGCCAG CAGCTGGGGC ACGTGGAAGC CTGGGCAGAG 
AAGCAGGGAT TAGAGCTGGC AGCCGACTGT CACCTGAGCC GGATAGTGCA GGCGACCACT 
TTGCTCACCA TGGACAAATA CGCTCCTGAG GACACCCCAA ATATCAACAG CACCTGCTTT 
AAGTTAAATT CGCTGCAGCT GCAGGCCTTG CTACAGAATT ACCACTGTGC GCCCGACGAG 
CCCTTTGTCC CCACGGACCT GATAGAGAAT GTCGTGGCCG TGGCCGAAAA CACGGCCGAC 
GAGCTGGCAC GCAGTGATGG GCGGGAGGTG CAGCTGGAAG AGGACCCTGA TCTGCAGCTG 
CCTTTTCTTT TGCCGGAAGA CGGCTATTCT TGTGACGTCG TCAGGAACGT TCCAAACGGT 
TTACAAGAAT TTCTGGACCC TCTGTGCCAG AGAGGGTTTT GCAGGTTAAT TCCTCATGCA 
CGTTCACCAG GTACTTGGAC GATATATTTT GAAGGTGCAG ATTATGAGAG CCATCTTCTG 
CGTGAGAACA CAGAGCTGGC TCAGCCTCTG CGGAAAGAAC CTGAAATAAT CACTGTGACT 
CTCAAAAAGC AAAACGGAAT GGGCCTCAGC ATTGTCGCAG CAAAGGGTGC TGGTCAAGAT 
AAACTTGGAA TCTATGTCAA GTCTGTCGTA AAAGGAGGTG CTGCAGATGT GGATGGACGC 
CTAGCCGCAG GTGACCAGCT CCTCAGTGTG GATGGACGAA GTCTGGTAGG ACTCTCTCAA 
GAAAGGGCAG CAGAACTCAT GACAAGGACC AGTTCCGTGG TGACACTGGA AGTAGCCAAG 
CAAGGAGCGA TCTACCACGG CCTGGCCACC CTGCTCAACC AGCCGTCCCC GATGATGCAG 
AGAGTTTCAG ACCGTCGTGG CTCAGGTAAA CCCCGACCAA AGAGCGAAGG TTTCGAGCTC 
TATAACAATT CAGCTCAAAA CGGGTCACCC GAGAGCCCTC AGCTGCCTTG GGCAGAATAC 
AGCGAACCGA AGAAGCTCCC TGGCGATGAC AGGCTGATGA AGAACCGGGC TGACCACCGC 
TCCAGCCCCA ACGTGGCAAA TCAGCCCCCC AGTCCCGGAG GGAAAAGCGC GTACGCCCCT 
GGAGCAGCCG CTAAGATCAC GTCCGTCTCC ACCGGAAACC TCTGCACTGA GGAGCAGACC 
CCTCCGCCTC GACCTGAGGC CTACCCCATC CCCACCCAGA CGTACACCAG GGAGTACTTC 
ACCTTCCCGG CCTCCAGGTC CCAGGACCGC GTGGCTCCCC CTCAGAACCA GTGGCCCAAT 
TACGAGGAGA AGCCGCACGT GCACACAGAT GGTCATCAGT CCGGCCCCGC GATGCAGCGC 
GTCACCCGCT CCCAGGAGGA ACTTCGGGAG GATAAAGCTT ACCAACTCGA GCGGCATCGG 
CTGGAGGCGG TGCTGGATCG CAAGTCCGAC AGTGACGTGT GGATCCAGCC GAGCTCCTCG 
GTGGGCTCCA GCACGTCCAG CCAGGAGCAC CTGGACCCCT GCTCCAAGTC GGGCACCCCC 
GCCTCCACGC TGACCAAGAG CGGCCCCGGG CGCTGGAAGA CGCCAGCCTC CGCGCAGCCC 
CTGCCGGCGG CCCCCCCGCC CGCGCAGACA GACCTGCCCC CGCCACCCCC GCCGCCCCCC 
GCGCACTACG CCGGAGACTT CGACGGGATC CCCGTGGACC TGCCCCTCCC GCCCCCTCCC 
AGCGGCCAGG CGGCGGCGGA GCGGAGGAAA CGGGAGGAGC AGCAGCGCTG GTACGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAC AGGAAGCGCC GGGAGCAGGA GCGGAAGCTG 
GGCCAGATGC GCTCCCAGGC CCTGAACCCG GCCGCGTTCT CCCCAGTGAC CGTCGCGCAC 
ACGAAGCCGG AAAAACCTTC CACGCTCCAG AGGCCCCAGG ACACGGTCAT CCGGGAGCTG 
CAGCCCCAGC AGCAGCCACG CACCATCGAG CGCCGAGACT TGCAGTACAT CACGGTCAGC 
AAAGAGGAGC TGTCCTCGGG GGACAGCCTG TCCCCCGACC CCTGGAAGCG GGACGCCAGG 
GAGAAGCTGG AGAAGCAGCA GCAAATGCAC ATCGTGGACA TGCTGAGCAG GGAGATCCGG 
GAGCTCCAGA GCAAGCCCGA GCGCAGCGCC GAGGACAGCG ACCGCCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTGCAG GAGTCCAAGC AGAAGGACGA GGACGACGAG 
GAGGAGGAGG ACGACGACGT GGACACCATG CTGATCATGC AGCGCCTCGA GGCTGAGCGG 
AGAGCAAGGG ACGAGGAGCG GCGGCGGCAG CGGCAGCTGG ACGACATGCG CAGGCGGGAA 
GCGGAGGACC GCGCCAGGCA GGAGGAGGAG AGGACGAAGC GAGACGCCGA AGACAAGAGG 
CGACAGGAAG AAGGTTATTA CAGCCGCCTG GAAGCCGAGA GGCGCAGACA GCACGCGGAG 
GCCGAGCGCA GGCTGCTGGA GCCGGAGGAG CCGGGCCTGG GCAGACCGCC GCTGCCGCGG 
GGCTACGAGC TCCCGGCCCC GTCCTCCCCG GCGGCTGCTC CTCCTCCCCC ACCTCAGCGG 
ACCGCCTCCT CCCTGCAGGC CCCGGCCCTG TCCCCAGACT CCCTGTACAC CGCCAGGCTC 
GTTGCGTACC ACGAGGAGGA GGAGGAGGAG GACTGCAGTC TGGCAGGTCA GGACAAGCTC 
TCCCACACCG GACCAAACTC TTCCGTGGGA TCTTCCGGGG CAGCGATCGG TGCCCGTGAC 
GGCTCCCGGG ACCCGAGAGA GAGGCTGGCG AAAAGCCATG ATGCAGATTT ACCTGGAAAT 
TCCGGAGCCC CTGAGAACTT GACATTCAAA GAGCGCCAGC GGCTGTTCTC ACAAGGTCAA 
GATGTGTCCA ATAAAGTGAA AGCCTCTCGT AAATTAACAG AACTGGAAAA CGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254586.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110354.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

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CloneID	OFb76145
Clone ID Related Accession (Same CDS sequence)	XM_023254586.1
Accession Version	XM_023254586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG ATGGTTCTGA CTCCAGGGAC 
AAGCCCAAGC TTTACCGCCT TCAGTTGAGC GTTACTGAGG TCGGGACAGA GAAGTTCGAG 
GAGAACTCCA TCCAGCTGTT TGGATCAGGA ATTCAGCCCC ATCACTGTGA CCTCACAAAC 
ATGGATGGAG TTGTCACCGT GACTCCGAGA AGCATGGACG CCGAGACGTA CGTGGAAGGA 
CAGCGCATCT CCGAGACAAC CATGCTGCAG AGTGGGATGA AAGTGCAGTT TGGGGCGTCA 
CACGTGTTCA AGTTTGTGGA CCCCAGCCAG GACCACGCAG TGGCCAAGAG ACCTGTGGAT 
GGAGGCCTGA CGGCCAAGGG CCCCAGACAG AAGCCTGGAA TTGTTCAGGA GACAACTTTC 
GATTTGGGGG GAGATGTTCA CAGCGGGGCA GCGCTGCCAG CGAGCAAGAG CGCCACCAGG 
CTGGACGGCG ACAGGGCACT GCCTGCCCCC AGCACGGCCG AGCGCGGGAC AGTGAAGCCG 
ATGCTGAGAC TGGAGCAGCA GGACTACCGC CGGCAAGAAA ACAGAACTCA GGATGCTCTT 
GGGCCTGAAC TCATACTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCATTT 
TTATCTGCCA TTATAAATTA TACTAATAGC TCTACAGTCC ATTTTAAGTT GTCACCTACA 
TATGTATTAT ATATGGCATG TCGGTATGTA TTGTCGAGCC AGTACAGACC CGATGTCAGT 
CCTACAGAGC GTACTCACAA AGTGATTGCC ATAGTCAACA AGATGGTGAG CATGATGGAG 
GGGGTGATTC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAATATCGC AGGGGCACTT 
GCTTTCTGGA TGGCCAATGC ATCTGAGCTT CTCAACTTCA TTAAGCAGGA CCGAGATCTT 
AGTCGAATCA CATTGGATGC CCAAGATGTT TTAGCACACT TGGTTCAAAT GGCGTTTAAA 
TATCTGGTTC ATTGTCTTCA GTCAGAACTG AATAACTACA TGCCAGCTTT TCTGGATGAC 
CCTGAAGAGA ACAGTCTACA AAGACCAAAA ATAGATGACG TCTTGCACAC GCTCACGGGA 
GCGATGGCGC TGCTGAGGCG CTGCAGGGTC AATGCTGCTC TGACCATCCA GCTTTTCTCT 
CAGCTCTTCC ACTTCATCAA CATGTGGCTC TTCAACAGAT TGGTGACCGA CCCCGAGTCG 
GGACTGTGCT CTCACTACTG GGGGGCAATC ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC 
TGGGCAGAGA AGCAGGGATT AGAGCTGGCA GCCGACTGTC ACCTGAGCCG GATAGTGCAG 
GCGACCACTT TGCTCACCAT GGACAAATAC GCTCCTGAGG ACACCCCAAA TATCAACAGC 
ACCTGCTTTA AGTTAAATTC GCTGCAGCTG CAGGCCTTGC TACAGAATTA CCACTGTGCG 
CCCGACGAGC CCTTTGTCCC CACGGACCTG ATAGAGAATG TCGTGGCCGT GGCCGAAAAC 
ACGGCCGACG AGCTGGCACG CAGTGATGGG CGGGAGGTGC AGCTGGAAGA GGACCCTGAT 
CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC GGCTATTCTT GTGACGTCGT CAGGAACGTT 
CCAAACGGTT TACAAGAATT TCTGGACCCT CTGTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCATGCAC GTTCACCAGG TACTTGGACG ATATATTTTG AAGGTGCAGA TTATGAGAGC 
CATCTTCTGC GTGAGAACAC AGAGCTGGCT CAGCCTCTGC GGAAAGAACC TGAAATAATC 
ACTGTGACTC TCAAAAAGCA AAACGGAATG GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT 
GGTCAAGATA AACTTGGAAT CTATGTCAAG TCTGTCGTAA AAGGAGGTGC TGCAGATGTG 
GATGGACGCC TAGCCGCAGG TGACCAGCTC CTCAGTGTGG ATGGACGAAG TCTGGTAGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTCATG ACAAGGACCA GTTCCGTGGT GACACTGGAA 
GTAGCCAAGC AAGGAGCGAT CTACCACGGC CTGGCCACCC TGCTCAACCA GCCGTCCCCG 
ATGATGCAGA GAGTTTCAGA CCGTCGTGGC TCAGGTAAAC CCCGACCAAA GAGCGAAGGT 
TTCGAGCTCT ATAACAATTC AGCTCAAAAC GGGTCACCCG AGAGCCCTCA GCTGCCTTGG 
GCAGAATACA GCGAACCGAA GAAGCTCCCT GGCGATGACA GGCTGATGAA GAACCGGGCT 
GACCACCGCT CCAGCCCCAA CGTGGCAAAT CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG 
TACGCCCCTG GAGCAGCCGC TAAGATCACG TCCGTCTCCA CCGGAAACCT CTGCACTGAG 
GAGCAGACCC CTCCGCCTCG ACCTGAGGCC TACCCCATCC CCACCCAGAC GTACACCAGG 
GAGTACTTCA CCTTCCCGGC CTCCAGGTCC CAGGACCGCG TGGCTCCCCC TCAGAACCAG 
TGGCCCAATT ACGAGGAGAA GCCGCACGTG CACACAGATG GTCATCAGTC CGGCCCCGCG 
ATGCAGCGCG TCACCCGCTC CCAGGAGGAA CTTCGGGAGG ATAAAGCTTA CCAACTCGAG 
CGGCATCGGC TGGAGGCGGT GCTGGATCGC AAGTCCGACA GTGACGTGTG GATCCAGCCG 
AGCTCCTCGG TGGGCTCCAG CACGTCCAGC CAGGAGCACC TGGACCCCTG CTCCAAGTCG 
GGCACCCCCG CCTCCACGCT GACCAAGAGC GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC 
GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC GCGCAGACAG ACCTGCCCCC GCCACCCCCG 
CCGCCCCCCG CGCACTACGC CGGAGACTTC GACGGGATCC CCGTGGACCT GCCCCTCCCG 
CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG 
TACGAGAAGG AGAAGGCCCG CCTGGAGGAG GAGCGGGACA GGAAGCGCCG GGAGCAGGAG 
CGGAAGCTGG GCCAGATGCG CTCCCAGGCC CTGAACCCGG CCGCGTTCTC CCCAGTGACC 
GTCGCGCACA CGAAGCCGGA AAAACCTTCC ACGCTCCAGA GGCCCCAGGA CACGGTCATC 
CGGGAGCTGC AGCCCCAGCA GCAGCCACGC ACCATCGAGC GCCGAGACTT GCAGTACATC 
ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG GACAGCCTGT CCCCCGACCC CTGGAAGCGG 
GACGCCAGGG AGAAGCTGGA GAAGCAGCAG CAAATGCACA TCGTGGACAT GCTGAGCAGG 
GAGATCCGGG AGCTCCAGAG CAAGCCCGAG CGCAGCGCCG AGGACAGCGA CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTCGAG 
GCTGAGCGGA GAGCAAGGCA GACTGGTCTG CCAGCAATCT CTGTTCTAGA TTTGTTACAG 
GACGAGGAGC GGCGGCGGCA GCGGCAGCTG GACGACATGC GCAGGCGGGA AGCGGAGGAC 
CGCGCCAGGC AGGAGGAGGA GAGGACGAAG CGAGACGCCG AAGACAAGAG GCGACAGGAA 
GAAGGTTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGCGGA GGCCGAGCGC 
AGGCTGCTGG AGCCGGAGGA GCCGGGCCTG GGCAGACCGC CGCTGCCGCG GGGCTACGAG 
CTCCCGGCCC CGTCCTCCCC GGCGGCTGCT CCTCCTCCCC CACCTCAGCG GACCGCCTCC 
TCCCTGCAGG CCCCGGCCCT GTCCCCAGAC TCCCTGTACA CCGCCAGGCT CGTTGCGTAC 
CACGAGGAGG AGGAGGAGGA GGACTGCAGT CTGGCAGGTC AGGACAAGCT CTCCCACACC 
GGACCAAACT CTTCCGTGGG ATCTTCCGGG GCAGCGATCG GTGCCCGTGA CGGCTCCCGG 
GACCCGAGAG AGAGGCTGGC GAAAAGCCAT GATGCAGATT TACCTGGAAA TTCCGGAGCC 
CCTGAGAACT TGACATTCAA AGAGCGCCAG CGGCTGTTCT CACAAGGTCA AGATGTGTCC 
AATAAAGTGA AAGCCTCTCG TAAATTAACA GAACTGGAAA ACGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110354.1
CDS	46..5505
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254586.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254586.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG ATGGTTCTGA CTCCAGGGAC 
AAGCCCAAGC TTTACCGCCT TCAGTTGAGC GTTACTGAGG TCGGGACAGA GAAGTTCGAG 
GAGAACTCCA TCCAGCTGTT TGGATCAGGA ATTCAGCCCC ATCACTGTGA CCTCACAAAC 
ATGGATGGAG TTGTCACCGT GACTCCGAGA AGCATGGACG CCGAGACGTA CGTGGAAGGA 
CAGCGCATCT CCGAGACAAC CATGCTGCAG AGTGGGATGA AAGTGCAGTT TGGGGCGTCA 
CACGTGTTCA AGTTTGTGGA CCCCAGCCAG GACCACGCAG TGGCCAAGAG ACCTGTGGAT 
GGAGGCCTGA CGGCCAAGGG CCCCAGACAG AAGCCTGGAA TTGTTCAGGA GACAACTTTC 
GATTTGGGGG GAGATGTTCA CAGCGGGGCA GCGCTGCCAG CGAGCAAGAG CGCCACCAGG 
CTGGACGGCG ACAGGGCACT GCCTGCCCCC AGCACGGCCG AGCGCGGGAC AGTGAAGCCG 
ATGCTGAGAC TGGAGCAGCA GGACTACCGC CGGCAAGAAA ACAGAACTCA GGATGCTCTT 
GGGCCTGAAC TCATACTGCC TGCAAGCATT GAATTCAGGG AAAGTTCTGA AGATTCATTT 
TTATCTGCCA TTATAAATTA TACTAATAGC TCTACAGTCC ATTTTAAGTT GTCACCTACA 
TATGTATTAT ATATGGCATG TCGGTATGTA TTGTCGAGCC AGTACAGACC CGATGTCAGT 
CCTACAGAGC GTACTCACAA AGTGATTGCC ATAGTCAACA AGATGGTGAG CATGATGGAG 
GGGGTGATTC AGGAAGTAGA CCAGGTTGAT CAGAAACAGA AGAATATCGC AGGGGCACTT 
GCTTTCTGGA TGGCCAATGC ATCTGAGCTT CTCAACTTCA TTAAGCAGGA CCGAGATCTT 
AGTCGAATCA CATTGGATGC CCAAGATGTT TTAGCACACT TGGTTCAAAT GGCGTTTAAA 
TATCTGGTTC ATTGTCTTCA GTCAGAACTG AATAACTACA TGCCAGCTTT TCTGGATGAC 
CCTGAAGAGA ACAGTCTACA AAGACCAAAA ATAGATGACG TCTTGCACAC GCTCACGGGA 
GCGATGGCGC TGCTGAGGCG CTGCAGGGTC AATGCTGCTC TGACCATCCA GCTTTTCTCT 
CAGCTCTTCC ACTTCATCAA CATGTGGCTC TTCAACAGAT TGGTGACCGA CCCCGAGTCG 
GGACTGTGCT CTCACTACTG GGGGGCAATC ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC 
TGGGCAGAGA AGCAGGGATT AGAGCTGGCA GCCGACTGTC ACCTGAGCCG GATAGTGCAG 
GCGACCACTT TGCTCACCAT GGACAAATAC GCTCCTGAGG ACACCCCAAA TATCAACAGC 
ACCTGCTTTA AGTTAAATTC GCTGCAGCTG CAGGCCTTGC TACAGAATTA CCACTGTGCG 
CCCGACGAGC CCTTTGTCCC CACGGACCTG ATAGAGAATG TCGTGGCCGT GGCCGAAAAC 
ACGGCCGACG AGCTGGCACG CAGTGATGGG CGGGAGGTGC AGCTGGAAGA GGACCCTGAT 
CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC GGCTATTCTT GTGACGTCGT CAGGAACGTT 
CCAAACGGTT TACAAGAATT TCTGGACCCT CTGTGCCAGA GAGGGTTTTG CAGGTTAATT 
CCTCATGCAC GTTCACCAGG TACTTGGACG ATATATTTTG AAGGTGCAGA TTATGAGAGC 
CATCTTCTGC GTGAGAACAC AGAGCTGGCT CAGCCTCTGC GGAAAGAACC TGAAATAATC 
ACTGTGACTC TCAAAAAGCA AAACGGAATG GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT 
GGTCAAGATA AACTTGGAAT CTATGTCAAG TCTGTCGTAA AAGGAGGTGC TGCAGATGTG 
GATGGACGCC TAGCCGCAGG TGACCAGCTC CTCAGTGTGG ATGGACGAAG TCTGGTAGGA 
CTCTCTCAAG AAAGGGCAGC AGAACTCATG ACAAGGACCA GTTCCGTGGT GACACTGGAA 
GTAGCCAAGC AAGGAGCGAT CTACCACGGC CTGGCCACCC TGCTCAACCA GCCGTCCCCG 
ATGATGCAGA GAGTTTCAGA CCGTCGTGGC TCAGGTAAAC CCCGACCAAA GAGCGAAGGT 
TTCGAGCTCT ATAACAATTC AGCTCAAAAC GGGTCACCCG AGAGCCCTCA GCTGCCTTGG 
GCAGAATACA GCGAACCGAA GAAGCTCCCT GGCGATGACA GGCTGATGAA GAACCGGGCT 
GACCACCGCT CCAGCCCCAA CGTGGCAAAT CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG 
TACGCCCCTG GAGCAGCCGC TAAGATCACG TCCGTCTCCA CCGGAAACCT CTGCACTGAG 
GAGCAGACCC CTCCGCCTCG ACCTGAGGCC TACCCCATCC CCACCCAGAC GTACACCAGG 
GAGTACTTCA CCTTCCCGGC CTCCAGGTCC CAGGACCGCG TGGCTCCCCC TCAGAACCAG 
TGGCCCAATT ACGAGGAGAA GCCGCACGTG CACACAGATG GTCATCAGTC CGGCCCCGCG 
ATGCAGCGCG TCACCCGCTC CCAGGAGGAA CTTCGGGAGG ATAAAGCTTA CCAACTCGAG 
CGGCATCGGC TGGAGGCGGT GCTGGATCGC AAGTCCGACA GTGACGTGTG GATCCAGCCG 
AGCTCCTCGG TGGGCTCCAG CACGTCCAGC CAGGAGCACC TGGACCCCTG CTCCAAGTCG 
GGCACCCCCG CCTCCACGCT GACCAAGAGC GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC 
GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC GCGCAGACAG ACCTGCCCCC GCCACCCCCG 
CCGCCCCCCG CGCACTACGC CGGAGACTTC GACGGGATCC CCGTGGACCT GCCCCTCCCG 
CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG 
TACGAGAAGG AGAAGGCCCG CCTGGAGGAG GAGCGGGACA GGAAGCGCCG GGAGCAGGAG 
CGGAAGCTGG GCCAGATGCG CTCCCAGGCC CTGAACCCGG CCGCGTTCTC CCCAGTGACC 
GTCGCGCACA CGAAGCCGGA AAAACCTTCC ACGCTCCAGA GGCCCCAGGA CACGGTCATC 
CGGGAGCTGC AGCCCCAGCA GCAGCCACGC ACCATCGAGC GCCGAGACTT GCAGTACATC 
ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG GACAGCCTGT CCCCCGACCC CTGGAAGCGG 
GACGCCAGGG AGAAGCTGGA GAAGCAGCAG CAAATGCACA TCGTGGACAT GCTGAGCAGG 
GAGATCCGGG AGCTCCAGAG CAAGCCCGAG CGCAGCGCCG AGGACAGCGA CCGCCTGCGC 
AAGCTCATGC TGGAGTGGCA GTTCCAGAAG AGACTGCAGG AGTCCAAGCA GAAGGACGAG 
GACGACGAGG AGGAGGAGGA CGACGACGTG GACACCATGC TGATCATGCA GCGCCTCGAG 
GCTGAGCGGA GAGCAAGGCA GACTGGTCTG CCAGCAATCT CTGTTCTAGA TTTGTTACAG 
GACGAGGAGC GGCGGCGGCA GCGGCAGCTG GACGACATGC GCAGGCGGGA AGCGGAGGAC 
CGCGCCAGGC AGGAGGAGGA GAGGACGAAG CGAGACGCCG AAGACAAGAG GCGACAGGAA 
GAAGGTTATT ACAGCCGCCT GGAAGCCGAG AGGCGCAGAC AGCACGCGGA GGCCGAGCGC 
AGGCTGCTGG AGCCGGAGGA GCCGGGCCTG GGCAGACCGC CGCTGCCGCG GGGCTACGAG 
CTCCCGGCCC CGTCCTCCCC GGCGGCTGCT CCTCCTCCCC CACCTCAGCG GACCGCCTCC 
TCCCTGCAGG CCCCGGCCCT GTCCCCAGAC TCCCTGTACA CCGCCAGGCT CGTTGCGTAC 
CACGAGGAGG AGGAGGAGGA GGACTGCAGT CTGGCAGGTC AGGACAAGCT CTCCCACACC 
GGACCAAACT CTTCCGTGGG ATCTTCCGGG GCAGCGATCG GTGCCCGTGA CGGCTCCCGG 
GACCCGAGAG AGAGGCTGGC GAAAAGCCAT GATGCAGATT TACCTGGAAA TTCCGGAGCC 
CCTGAGAACT TGACATTCAA AGAGCGCCAG CGGCTGTTCT CACAAGGTCA AGATGTGTCC 
AATAAAGTGA AAGCCTCTCG TAAATTAACA GAACTGGAAA ACGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254587.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110355.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OFb76146
Clone ID Related Accession (Same CDS sequence)	XM_023254587.1
Accession Version	XM_023254587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTTGCTGT GGCTGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGATGGTT CTGACTCCAG GGACAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTCGGGA CAGAGAAGTT CGAGGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGAGTTGTCA CCGTGACTCC GAGAAGCATG GACGCCGAGA CGTACGTGGA AGGACAGCGC 
ATCTCCGAGA CAACCATGCT GCAGAGTGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCAGTGGCCA AGAGACCTGT GGATGGAGGC 
CTGACGGCCA AGGGCCCCAG ACAGAAGCCT GGAATTGTTC AGGAGACAAC TTTCGATTTG 
GGGGGAGATG TTCACAGCGG GGCAGCGCTG CCAGCGAGCA AGAGCGCCAC CAGGCTGGAC 
GGCGACAGGG CACTGCCTGC CCCCAGCACG GCCGAGCGCG GGACAGTGAA GCCGATGCTG 
AGACTGGAGC AGCAGGACTA CCGCCGGCAA GAAAACAGAA CTCAGGATGC TCTTGGGCCT 
GAACTCATAC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC ATTTTTATCT 
GCCATTATAA ATTATACTAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTA 
TTATATATGG CATGTCGGTA TGTATTGTCG AGCCAGTACA GACCCGATGT CAGTCCTACA 
GAGCGTACTC ACAAAGTGAT TGCCATAGTC AACAAGATGG TGAGCATGAT GGAGGGGGTG 
ATTCAGAAAC AGAAGAATAT CGCAGGGGCA CTTGCTTTCT GGATGGCCAA TGCATCTGAG 
CTTCTCAACT TCATTAAGCA GGACCGAGAT CTTAGTCGAA TCACATTGGA TGCCCAAGAT 
GTTTTAGCAC ACTTGGTTCA AATGGCGTTT AAATATCTGG TTCATTGTCT TCAGTCAGAA 
CTGAATAACT ACATGCCAGC TTTTCTGGAT GACCCTGAAG AGAACAGTCT ACAAAGACCA 
AAAATAGATG ACGTCTTGCA CACGCTCACG GGAGCGATGG CGCTGCTGAG GCGCTGCAGG 
GTCAATGCTG CTCTGACCAT CCAGCTTTTC TCTCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA GATTGGTGAC CGACCCCGAG TCGGGACTGT GCTCTCACTA CTGGGGGGCA 
ATCATCCGCC AGCAGCTGGG GCACGTGGAA GCCTGGGCAG AGAAGCAGGG ATTAGAGCTG 
GCAGCCGACT GTCACCTGAG CCGGATAGTG CAGGCGACCA CTTTGCTCAC CATGGACAAA 
TACGCTCCTG AGGACACCCC AAATATCAAC AGCACCTGCT TTAAGTTAAA TTCGCTGCAG 
CTGCAGGCCT TGCTACAGAA TTACCACTGT GCGCCCGACG AGCCCTTTGT CCCCACGGAC 
CTGATAGAGA ATGTCGTGGC CGTGGCCGAA AACACGGCCG ACGAGCTGGC ACGCAGTGAT 
GGGCGGGAGG TGCAGCTGGA AGAGGACCCT GATCTGCAGC TGCCTTTTCT TTTGCCGGAA 
GACGGCTATT CTTGTGACGT CGTCAGGAAC GTTCCAAACG GTTTACAAGA ATTTCTGGAC 
CCTCTGTGCC AGAGAGGGTT TTGCAGGTTA ATTCCTCATG CACGTTCACC AGGTACTTGG 
ACGATATATT TTGAAGGTGC AGATTATGAG AGCCATCTTC TGCGTGAGAA CACAGAGCTG 
GCTCAGCCTC TGCGGAAAGA ACCTGAAATA ATCACTGTGA CTCTCAAAAA GCAAAACGGA 
ATGGGCCTCA GCATTGTCGC AGCAAAGGGT GCTGGTCAAG ATAAACTTGG AATCTATGTC 
AAGTCTGTCG TAAAAGGAGG TGCTGCAGAT GTGGATGGAC GCCTAGCCGC AGGTGACCAG 
CTCCTCAGTG TGGATGGACG AAGTCTGGTA GGACTCTCTC AAGAAAGGGC AGCAGAACTC 
ATGACAAGGA CCAGTTCCGT GGTGACACTG GAAGTAGCCA AGCAAGGAGC GATCTACCAC 
GGCCTGGCCA CCCTGCTCAA CCAGCCGTCC CCGATGATGC AGAGAGTTTC AGACCGTCGT 
GGCTCAGGTA AACCCCGACC AAAGAGCGAA GGTTTCGAGC TCTATAACAA TTCAGCTCAA 
AACGGGTCAC CCGAGAGCCC TCAGCTGCCT TGGGCAGAAT ACAGCGAACC GAAGAAGCTC 
CCTGGCGATG ACAGGCTGAT GAAGAACCGG GCTGACCACC GCTCCAGCCC CAACGTGGCA 
AATCAGCCCC CCAGTCCCGG AGGGAAAAGC GCGTACGCCC CTGGAGCAGC CGCTAAGATC 
ACGTCCGTCT CCACCGGAAA CCTCTGCACT GAGGAGCAGA CCCCTCCGCC TCGACCTGAG 
GCCTACCCCA TCCCCACCCA GACGTACACC AGGGAGTACT TCACCTTCCC GGCCTCCAGG 
TCCCAGGACC GCGTGGCTCC CCCTCAGAAC CAGTGGCCCA ATTACGAGGA GAAGCCGCAC 
GTGCACACAG ATGGTCATCA GTCCGGCCCC GCGATGCAGC GCGTCACCCG CTCCCAGGAG 
GAACTTCGGG AGGATAAAGC TTACCAACTC GAGCGGCATC GGCTGGAGGC GGTGCTGGAT 
CGCAAGTCCG ACAGTGACGT GTGGATCCAG CCGAGCTCCT CGGTGGGCTC CAGCACGTCC 
AGCCAGGAGC ACCTGGACCC CTGCTCCAAG TCGGGCACCC CCGCCTCCAC GCTGACCAAG 
AGCGGCCCCG GGCGCTGGAA GACGCCAGCC TCCGCGCAGC CCCTGCCGGC GGCCCCCCCG 
CCCGCGCAGA CAGACCTGCC CCCGCCACCC CCGCCGCCCC CCGCGCACTA CGCCGGAGAC 
TTCGACGGGA TCCCCGTGGA CCTGCCCCTC CCGCCCCCTC CCAGCGGCCA GGCGGCGGCG 
GAGCGGAGGA AACGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG ACAGGAAGCG CCGGGAGCAG GAGCGGAAGC TGGGCCAGAT GCGCTCCCAG 
GCCCTGAACC CGGCCGCGTT CTCCCCAGTG ACCGTCGCGC ACACGAAGCC GGAAAAACCT 
TCCACGCTCC AGAGGCCCCA GGACACGGTC ATCCGGGAGC TGCAGCCCCA GCAGCAGCCA 
CGCACCATCG AGCGCCGAGA CTTGCAGTAC ATCACGGTCA GCAAAGAGGA GCTGTCCTCG 
GGGGACAGCC TGTCCCCCGA CCCCTGGAAG CGGGACGCCA GGGAGAAGCT GGAGAAGCAG 
CAGCAAATGC ACATCGTGGA CATGCTGAGC AGGGAGATCC GGGAGCTCCA GAGCAAGCCC 
GAGCGCAGCG CCGAGGACAG CGACCGCCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGACGAC 
GTGGACACCA TGCTGATCAT GCAGCGCCTC GAGGCTGAGC GGAGAGCAAG GCAGACTGGT 
CTGCCAGCAA TCTCTGTTCT AGATTTGTTA CAGGACGAGG AGCGGCGGCG GCAGCGGCAG 
CTGGACGACA TGCGCAGGCG GGAAGCGGAG GACCGCGCCA GGCAGGAGGA GGAGAGGACG 
AAGCGAGACG CCGAAGACAA GAGGCGACAG GAAGAAGGTT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGC GGAGGCCGAG CGCAGGCTGC TGGAGCCGGA GGAGCCGGGC 
CTGGGCAGAC CGCCGCTGCC GCGGGGCTAC GAGCTCCCGG CCCCGTCCTC CCCGGCGGCT 
GCTCCTCCTC CCCCACCTCA GCGGACCGCC TCCTCCCTGC AGGCCCCGGC CCTGTCCCCA 
GACTCCCTGT ACACCGCCAG GCTCGTTGCG TACCACGAGG AGGAGGAGGA GGAGGACTGC 
AGTCTGGCAG GTCAGGACAA GCTCTCCCAC ACCGGACCAA ACTCTTCCGT GGGATCTTCC 
GGGGCAGCGA TCGGTGCCCG TGACGGCTCC CGGGACCCGA GAGAGAGGCT GGCGAAAAGC 
CATGATGCAG ATTTACCTGG AAATTCCGGA GCCCCTGAGA ACTTGACATT CAAAGAGCGC 
CAGCGGCTGT TCTCACAAGG TCAAGATGTG TCCAATAAAG TGAAAGCCTC TCGTAAATTA 
ACAGAACTGG AAAACGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110355.1
CDS	47..5479
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARQTGLPAISVLDLLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGQDKLSHTGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254587.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254587.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTTGCTGT GGCTGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGATGGTT CTGACTCCAG GGACAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTCGGGA CAGAGAAGTT CGAGGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGAGTTGTCA CCGTGACTCC GAGAAGCATG GACGCCGAGA CGTACGTGGA AGGACAGCGC 
ATCTCCGAGA CAACCATGCT GCAGAGTGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCAGTGGCCA AGAGACCTGT GGATGGAGGC 
CTGACGGCCA AGGGCCCCAG ACAGAAGCCT GGAATTGTTC AGGAGACAAC TTTCGATTTG 
GGGGGAGATG TTCACAGCGG GGCAGCGCTG CCAGCGAGCA AGAGCGCCAC CAGGCTGGAC 
GGCGACAGGG CACTGCCTGC CCCCAGCACG GCCGAGCGCG GGACAGTGAA GCCGATGCTG 
AGACTGGAGC AGCAGGACTA CCGCCGGCAA GAAAACAGAA CTCAGGATGC TCTTGGGCCT 
GAACTCATAC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC ATTTTTATCT 
GCCATTATAA ATTATACTAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTA 
TTATATATGG CATGTCGGTA TGTATTGTCG AGCCAGTACA GACCCGATGT CAGTCCTACA 
GAGCGTACTC ACAAAGTGAT TGCCATAGTC AACAAGATGG TGAGCATGAT GGAGGGGGTG 
ATTCAGAAAC AGAAGAATAT CGCAGGGGCA CTTGCTTTCT GGATGGCCAA TGCATCTGAG 
CTTCTCAACT TCATTAAGCA GGACCGAGAT CTTAGTCGAA TCACATTGGA TGCCCAAGAT 
GTTTTAGCAC ACTTGGTTCA AATGGCGTTT AAATATCTGG TTCATTGTCT TCAGTCAGAA 
CTGAATAACT ACATGCCAGC TTTTCTGGAT GACCCTGAAG AGAACAGTCT ACAAAGACCA 
AAAATAGATG ACGTCTTGCA CACGCTCACG GGAGCGATGG CGCTGCTGAG GCGCTGCAGG 
GTCAATGCTG CTCTGACCAT CCAGCTTTTC TCTCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA GATTGGTGAC CGACCCCGAG TCGGGACTGT GCTCTCACTA CTGGGGGGCA 
ATCATCCGCC AGCAGCTGGG GCACGTGGAA GCCTGGGCAG AGAAGCAGGG ATTAGAGCTG 
GCAGCCGACT GTCACCTGAG CCGGATAGTG CAGGCGACCA CTTTGCTCAC CATGGACAAA 
TACGCTCCTG AGGACACCCC AAATATCAAC AGCACCTGCT TTAAGTTAAA TTCGCTGCAG 
CTGCAGGCCT TGCTACAGAA TTACCACTGT GCGCCCGACG AGCCCTTTGT CCCCACGGAC 
CTGATAGAGA ATGTCGTGGC CGTGGCCGAA AACACGGCCG ACGAGCTGGC ACGCAGTGAT 
GGGCGGGAGG TGCAGCTGGA AGAGGACCCT GATCTGCAGC TGCCTTTTCT TTTGCCGGAA 
GACGGCTATT CTTGTGACGT CGTCAGGAAC GTTCCAAACG GTTTACAAGA ATTTCTGGAC 
CCTCTGTGCC AGAGAGGGTT TTGCAGGTTA ATTCCTCATG CACGTTCACC AGGTACTTGG 
ACGATATATT TTGAAGGTGC AGATTATGAG AGCCATCTTC TGCGTGAGAA CACAGAGCTG 
GCTCAGCCTC TGCGGAAAGA ACCTGAAATA ATCACTGTGA CTCTCAAAAA GCAAAACGGA 
ATGGGCCTCA GCATTGTCGC AGCAAAGGGT GCTGGTCAAG ATAAACTTGG AATCTATGTC 
AAGTCTGTCG TAAAAGGAGG TGCTGCAGAT GTGGATGGAC GCCTAGCCGC AGGTGACCAG 
CTCCTCAGTG TGGATGGACG AAGTCTGGTA GGACTCTCTC AAGAAAGGGC AGCAGAACTC 
ATGACAAGGA CCAGTTCCGT GGTGACACTG GAAGTAGCCA AGCAAGGAGC GATCTACCAC 
GGCCTGGCCA CCCTGCTCAA CCAGCCGTCC CCGATGATGC AGAGAGTTTC AGACCGTCGT 
GGCTCAGGTA AACCCCGACC AAAGAGCGAA GGTTTCGAGC TCTATAACAA TTCAGCTCAA 
AACGGGTCAC CCGAGAGCCC TCAGCTGCCT TGGGCAGAAT ACAGCGAACC GAAGAAGCTC 
CCTGGCGATG ACAGGCTGAT GAAGAACCGG GCTGACCACC GCTCCAGCCC CAACGTGGCA 
AATCAGCCCC CCAGTCCCGG AGGGAAAAGC GCGTACGCCC CTGGAGCAGC CGCTAAGATC 
ACGTCCGTCT CCACCGGAAA CCTCTGCACT GAGGAGCAGA CCCCTCCGCC TCGACCTGAG 
GCCTACCCCA TCCCCACCCA GACGTACACC AGGGAGTACT TCACCTTCCC GGCCTCCAGG 
TCCCAGGACC GCGTGGCTCC CCCTCAGAAC CAGTGGCCCA ATTACGAGGA GAAGCCGCAC 
GTGCACACAG ATGGTCATCA GTCCGGCCCC GCGATGCAGC GCGTCACCCG CTCCCAGGAG 
GAACTTCGGG AGGATAAAGC TTACCAACTC GAGCGGCATC GGCTGGAGGC GGTGCTGGAT 
CGCAAGTCCG ACAGTGACGT GTGGATCCAG CCGAGCTCCT CGGTGGGCTC CAGCACGTCC 
AGCCAGGAGC ACCTGGACCC CTGCTCCAAG TCGGGCACCC CCGCCTCCAC GCTGACCAAG 
AGCGGCCCCG GGCGCTGGAA GACGCCAGCC TCCGCGCAGC CCCTGCCGGC GGCCCCCCCG 
CCCGCGCAGA CAGACCTGCC CCCGCCACCC CCGCCGCCCC CCGCGCACTA CGCCGGAGAC 
TTCGACGGGA TCCCCGTGGA CCTGCCCCTC CCGCCCCCTC CCAGCGGCCA GGCGGCGGCG 
GAGCGGAGGA AACGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG ACAGGAAGCG CCGGGAGCAG GAGCGGAAGC TGGGCCAGAT GCGCTCCCAG 
GCCCTGAACC CGGCCGCGTT CTCCCCAGTG ACCGTCGCGC ACACGAAGCC GGAAAAACCT 
TCCACGCTCC AGAGGCCCCA GGACACGGTC ATCCGGGAGC TGCAGCCCCA GCAGCAGCCA 
CGCACCATCG AGCGCCGAGA CTTGCAGTAC ATCACGGTCA GCAAAGAGGA GCTGTCCTCG 
GGGGACAGCC TGTCCCCCGA CCCCTGGAAG CGGGACGCCA GGGAGAAGCT GGAGAAGCAG 
CAGCAAATGC ACATCGTGGA CATGCTGAGC AGGGAGATCC GGGAGCTCCA GAGCAAGCCC 
GAGCGCAGCG CCGAGGACAG CGACCGCCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGACGAC 
GTGGACACCA TGCTGATCAT GCAGCGCCTC GAGGCTGAGC GGAGAGCAAG GCAGACTGGT 
CTGCCAGCAA TCTCTGTTCT AGATTTGTTA CAGGACGAGG AGCGGCGGCG GCAGCGGCAG 
CTGGACGACA TGCGCAGGCG GGAAGCGGAG GACCGCGCCA GGCAGGAGGA GGAGAGGACG 
AAGCGAGACG CCGAAGACAA GAGGCGACAG GAAGAAGGTT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGC GGAGGCCGAG CGCAGGCTGC TGGAGCCGGA GGAGCCGGGC 
CTGGGCAGAC CGCCGCTGCC GCGGGGCTAC GAGCTCCCGG CCCCGTCCTC CCCGGCGGCT 
GCTCCTCCTC CCCCACCTCA GCGGACCGCC TCCTCCCTGC AGGCCCCGGC CCTGTCCCCA 
GACTCCCTGT ACACCGCCAG GCTCGTTGCG TACCACGAGG AGGAGGAGGA GGAGGACTGC 
AGTCTGGCAG GTCAGGACAA GCTCTCCCAC ACCGGACCAA ACTCTTCCGT GGGATCTTCC 
GGGGCAGCGA TCGGTGCCCG TGACGGCTCC CGGGACCCGA GAGAGAGGCT GGCGAAAAGC 
CATGATGCAG ATTTACCTGG AAATTCCGGA GCCCCTGAGA ACTTGACATT CAAAGAGCGC 
CAGCGGCTGT TCTCACAAGG TCAAGATGTG TCCAATAAAG TGAAAGCCTC TCGTAAATTA 
ACAGAACTGG AAAACGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254588.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110356.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OFb76147
Clone ID Related Accession (Same CDS sequence)	XM_023254588.1
Accession Version	XM_023254588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGAAACAGA AGAATATCGC AGGGGCACTT GCTTTCTGGA TGGCCAATGC ATCTGAGCTT 
CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CATTGGATGC CCAAGATGTT 
TTAGCACACT TGGTTCAAAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA GTCAGAACTG 
AATAACTACA TGCCAGCTTT TCTGGATGAC CCTGAAGAGA ACAGTCTACA AAGACCAAAA 
ATAGATGACG TCTTGCACAC GCTCACGGGA GCGATGGCGC TGCTGAGGCG CTGCAGGGTC 
AATGCTGCTC TGACCATCCA GCTTTTCTCT CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAGAT TGGTGACCGA CCCCGAGTCG GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC TGGGCAGAGA AGCAGGGATT AGAGCTGGCA 
GCCGACTGTC ACCTGAGCCG GATAGTGCAG GCGACCACTT TGCTCACCAT GGACAAATAC 
GCTCCTGAGG ACACCCCAAA TATCAACAGC ACCTGCTTTA AGTTAAATTC GCTGCAGCTG 
CAGGCCTTGC TACAGAATTA CCACTGTGCG CCCGACGAGC CCTTTGTCCC CACGGACCTG 
ATAGAGAATG TCGTGGCCGT GGCCGAAAAC ACGGCCGACG AGCTGGCACG CAGTGATGGG 
CGGGAGGTGC AGCTGGAAGA GGACCCTGAT CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC 
GGCTATTCTT GTGACGTCGT CAGGAACGTT CCAAACGGTT TACAAGAATT TCTGGACCCT 
CTGTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCATGCAC GTTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CATCTTCTGC GTGAGAACAC AGAGCTGGCT 
CAGCCTCTGC GGAAAGAACC TGAAATAATC ACTGTGACTC TCAAAAAGCA AAACGGAATG 
GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG 
TCTGTCGTAA AAGGAGGTGC TGCAGATGTG GATGGACGCC TAGCCGCAGG TGACCAGCTC 
CTCAGTGTGG ATGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCAGC AGAACTCATG 
ACAAGGACCA GTTCCGTGGT GACACTGGAA GTAGCCAAGC AAGGAGCGAT CTACCACGGC 
CTGGCCACCC TGCTCAACCA GCCGTCCCCG ATGATGCAGA GAGTTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTCGAGCTCT ATAACAATTC AGCTCAAAAC 
GGGTCACCCG AGAGCCCTCA GCTGCCTTGG GCAGAATACA GCGAACCGAA GAAGCTCCCT 
GGCGATGACA GGCTGATGAA GAACCGGGCT GACCACCGCT CCAGCCCCAA CGTGGCAAAT 
CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG TACGCCCCTG GAGCAGCCGC TAAGATCACG 
TCCGTCTCCA CCGGAAACCT CTGCACTGAG GAGCAGACCC CTCCGCCTCG ACCTGAGGCC 
TACCCCATCC CCACCCAGAC GTACACCAGG GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGCTCCCCC TCAGAACCAG TGGCCCAATT ACGAGGAGAA GCCGCACGTG 
CACACAGATG GTCATCAGTC CGGCCCCGCG ATGCAGCGCG TCACCCGCTC CCAGGAGGAA 
CTTCGGGAGG ATAAAGCTTA CCAACTCGAG CGGCATCGGC TGGAGGCGGT GCTGGATCGC 
AAGTCCGACA GTGACGTGTG GATCCAGCCG AGCTCCTCGG TGGGCTCCAG CACGTCCAGC 
CAGGAGCACC TGGACCCCTG CTCCAAGTCG GGCACCCCCG CCTCCACGCT GACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC 
GCGCAGACAG ACCTGCCCCC GCCACCCCCG CCGCCCCCCG CGCACTACGC CGGAGACTTC 
GACGGGATCC CCGTGGACCT GCCCCTCCCG CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG 
CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGACA GGAAGCGCCG GGAGCAGGAG CGGAAGCTGG GCCAGATGCG CTCCCAGGCC 
CTGAACCCGG CCGCGTTCTC CCCAGTGACC GTCGCGCACA CGAAGCCGGA AAAACCTTCC 
ACGCTCCAGA GGCCCCAGGA CACGGTCATC CGGGAGCTGC AGCCCCAGCA GCAGCCACGC 
ACCATCGAGC GCCGAGACTT GCAGTACATC ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG 
GACAGCCTGT CCCCCGACCC CTGGAAGCGG GACGCCAGGG AGAAGCTGGA GAAGCAGCAG 
CAAATGCACA TCGTGGACAT GCTGAGCAGG GAGATCCGGG AGCTCCAGAG CAAGCCCGAG 
CGCAGCGCCG AGGACAGCGA CCGCCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAAGGACGAG GACGACGAGG AGGAGGAGGA CGACGACGTG 
GACACCATGC TGATCATGCA GCGCCTCGAG GCTGAGCGGA GAGCAAGGTT ACAGGACGAG 
GAGCGGCGGC GGCAGCGGCA GCTGGACGAC ATGCGCAGGC GGGAAGCGGA GGACCGCGCC 
AGGCAGGAGG AGGAGAGGAC GAAGCGAGAC GCCGAAGACA AGAGGCGACA GGAAGAAGGT 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGCTG 
CTGGAGCCGG AGGAGCCGGG CCTGGGCAGA CCGCCGCTGC CGCGGGGCTA CGAGCTCCCG 
GCCCCGTCCT CCCCGGCGGC TGCTCCTCCT CCCCCACCTC AGCGGACCGC CTCCTCCCTG 
CAGGCCCCGG CCCTGTCCCC AGACTCCCTG TACACCGCCA GGCTCGTTGC GTACCACGAG 
GAGGAGGAGG AGGAGGACTG CAGTCTGGCA GGACCAAACT CTTCCGTGGG ATCTTCCGGG 
GCAGCGATCG GTGCCCGTGA CGGCTCCCGG GACCCGAGAG AGAGGCTGGC GAAAAGCCAT 
GATGCAGATT TACCTGGAAA TTCCGGAGCC CCTGAGAACT TGACATTCAA AGAGCGCCAG 
CGGCTGTTCT CACAAGGTCA AGATGTGTCC AATAAAGTGA AAGCCTCTCG TAAATTAACA 
GAACTGGAAA ACGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110356.1
CDS	46..5475
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254588.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254588.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGAAACAGA AGAATATCGC AGGGGCACTT GCTTTCTGGA TGGCCAATGC ATCTGAGCTT 
CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CATTGGATGC CCAAGATGTT 
TTAGCACACT TGGTTCAAAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA GTCAGAACTG 
AATAACTACA TGCCAGCTTT TCTGGATGAC CCTGAAGAGA ACAGTCTACA AAGACCAAAA 
ATAGATGACG TCTTGCACAC GCTCACGGGA GCGATGGCGC TGCTGAGGCG CTGCAGGGTC 
AATGCTGCTC TGACCATCCA GCTTTTCTCT CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAGAT TGGTGACCGA CCCCGAGTCG GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC TGGGCAGAGA AGCAGGGATT AGAGCTGGCA 
GCCGACTGTC ACCTGAGCCG GATAGTGCAG GCGACCACTT TGCTCACCAT GGACAAATAC 
GCTCCTGAGG ACACCCCAAA TATCAACAGC ACCTGCTTTA AGTTAAATTC GCTGCAGCTG 
CAGGCCTTGC TACAGAATTA CCACTGTGCG CCCGACGAGC CCTTTGTCCC CACGGACCTG 
ATAGAGAATG TCGTGGCCGT GGCCGAAAAC ACGGCCGACG AGCTGGCACG CAGTGATGGG 
CGGGAGGTGC AGCTGGAAGA GGACCCTGAT CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC 
GGCTATTCTT GTGACGTCGT CAGGAACGTT CCAAACGGTT TACAAGAATT TCTGGACCCT 
CTGTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCATGCAC GTTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CATCTTCTGC GTGAGAACAC AGAGCTGGCT 
CAGCCTCTGC GGAAAGAACC TGAAATAATC ACTGTGACTC TCAAAAAGCA AAACGGAATG 
GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG 
TCTGTCGTAA AAGGAGGTGC TGCAGATGTG GATGGACGCC TAGCCGCAGG TGACCAGCTC 
CTCAGTGTGG ATGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCAGC AGAACTCATG 
ACAAGGACCA GTTCCGTGGT GACACTGGAA GTAGCCAAGC AAGGAGCGAT CTACCACGGC 
CTGGCCACCC TGCTCAACCA GCCGTCCCCG ATGATGCAGA GAGTTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTCGAGCTCT ATAACAATTC AGCTCAAAAC 
GGGTCACCCG AGAGCCCTCA GCTGCCTTGG GCAGAATACA GCGAACCGAA GAAGCTCCCT 
GGCGATGACA GGCTGATGAA GAACCGGGCT GACCACCGCT CCAGCCCCAA CGTGGCAAAT 
CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG TACGCCCCTG GAGCAGCCGC TAAGATCACG 
TCCGTCTCCA CCGGAAACCT CTGCACTGAG GAGCAGACCC CTCCGCCTCG ACCTGAGGCC 
TACCCCATCC CCACCCAGAC GTACACCAGG GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGCTCCCCC TCAGAACCAG TGGCCCAATT ACGAGGAGAA GCCGCACGTG 
CACACAGATG GTCATCAGTC CGGCCCCGCG ATGCAGCGCG TCACCCGCTC CCAGGAGGAA 
CTTCGGGAGG ATAAAGCTTA CCAACTCGAG CGGCATCGGC TGGAGGCGGT GCTGGATCGC 
AAGTCCGACA GTGACGTGTG GATCCAGCCG AGCTCCTCGG TGGGCTCCAG CACGTCCAGC 
CAGGAGCACC TGGACCCCTG CTCCAAGTCG GGCACCCCCG CCTCCACGCT GACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC 
GCGCAGACAG ACCTGCCCCC GCCACCCCCG CCGCCCCCCG CGCACTACGC CGGAGACTTC 
GACGGGATCC CCGTGGACCT GCCCCTCCCG CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG 
CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGACA GGAAGCGCCG GGAGCAGGAG CGGAAGCTGG GCCAGATGCG CTCCCAGGCC 
CTGAACCCGG CCGCGTTCTC CCCAGTGACC GTCGCGCACA CGAAGCCGGA AAAACCTTCC 
ACGCTCCAGA GGCCCCAGGA CACGGTCATC CGGGAGCTGC AGCCCCAGCA GCAGCCACGC 
ACCATCGAGC GCCGAGACTT GCAGTACATC ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG 
GACAGCCTGT CCCCCGACCC CTGGAAGCGG GACGCCAGGG AGAAGCTGGA GAAGCAGCAG 
CAAATGCACA TCGTGGACAT GCTGAGCAGG GAGATCCGGG AGCTCCAGAG CAAGCCCGAG 
CGCAGCGCCG AGGACAGCGA CCGCCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAAGGACGAG GACGACGAGG AGGAGGAGGA CGACGACGTG 
GACACCATGC TGATCATGCA GCGCCTCGAG GCTGAGCGGA GAGCAAGGTT ACAGGACGAG 
GAGCGGCGGC GGCAGCGGCA GCTGGACGAC ATGCGCAGGC GGGAAGCGGA GGACCGCGCC 
AGGCAGGAGG AGGAGAGGAC GAAGCGAGAC GCCGAAGACA AGAGGCGACA GGAAGAAGGT 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG CGGAGGCCGA GCGCAGGCTG 
CTGGAGCCGG AGGAGCCGGG CCTGGGCAGA CCGCCGCTGC CGCGGGGCTA CGAGCTCCCG 
GCCCCGTCCT CCCCGGCGGC TGCTCCTCCT CCCCCACCTC AGCGGACCGC CTCCTCCCTG 
CAGGCCCCGG CCCTGTCCCC AGACTCCCTG TACACCGCCA GGCTCGTTGC GTACCACGAG 
GAGGAGGAGG AGGAGGACTG CAGTCTGGCA GGACCAAACT CTTCCGTGGG ATCTTCCGGG 
GCAGCGATCG GTGCCCGTGA CGGCTCCCGG GACCCGAGAG AGAGGCTGGC GAAAAGCCAT 
GATGCAGATT TACCTGGAAA TTCCGGAGCC CCTGAGAACT TGACATTCAA AGAGCGCCAG 
CGGCTGTTCT CACAAGGTCA AGATGTGTCC AATAAAGTGA AAGCCTCTCG TAAATTAACA 
GAACTGGAAA ACGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254589.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110357.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OFb76148
Clone ID Related Accession (Same CDS sequence)	XM_023254589.1
Accession Version	XM_023254589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGAAACAGA AGAATATCGC AGGGGCACTT GCTTTCTGGA TGGCCAATGC ATCTGAGCTT 
CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CATTGGATGC CCAAGATGTT 
TTAGCACACT TGGTTCAAAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA GTCAGAACTG 
AATAACTACA TGCCAGCTTT TCTGGATGAC CCTGAAGAGA ACAGTCTACA AAGACCAAAA 
ATAGATGACG TCTTGCACAC GCTCACGGGA GCGATGGCGC TGCTGAGGCG CTGCAGGGTC 
AATGCTGCTC TGACCATCCA GCTTTTCTCT CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAGAT TGGTGACCGA CCCCGAGTCG GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC TGGGCAGAGA AGCAGGGATT AGAGCTGGCA 
GCCGACTGTC ACCTGAGCCG GATAGTGCAG GCGACCACTT TGCTCACCAT GGACAAATAC 
GCTCCTGAGG ACACCCCAAA TATCAACAGC ACCTGCTTTA AGTTAAATTC GCTGCAGCTG 
CAGGCCTTGC TACAGAATTA CCACTGTGCG CCCGACGAGC CCTTTGTCCC CACGGACCTG 
ATAGAGAATG TCGTGGCCGT GGCCGAAAAC ACGGCCGACG AGCTGGCACG CAGTGATGGG 
CGGGAGGTGC AGCTGGAAGA GGACCCTGAT CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC 
GGCTATTCTT GTGACGTCGT CAGGAACGTT CCAAACGGTT TACAAGAATT TCTGGACCCT 
CTGTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCATGCAC GTTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CATCTTCTGC GTGAGAACAC AGAGCTGGCT 
CAGCCTCTGC GGAAAGAACC TGAAATAATC ACTGTGACTC TCAAAAAGCA AAACGGAATG 
GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG 
TCTGTCGTAA AAGGAGGTGC TGCAGATGTG GATGGACGCC TAGCCGCAGG TGACCAGCTC 
CTCAGTGTGG ATGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCAGC AGAACTCATG 
ACAAGGACCA GTTCCGTGGT GACACTGGAA GTAGCCAAGC AAGGAGCGAT CTACCACGGC 
CTGGCCACCC TGCTCAACCA GCCGTCCCCG ATGATGCAGA GAGTTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTCGAGCTCT ATAACAATTC AGCTCAAAAC 
GGGTCACCCG AGAGCCCTCA GCTGCCTTGG GCAGAATACA GCGAACCGAA GAAGCTCCCT 
GGCGATGACA GGCTGATGAA GAACCGGGCT GACCACCGCT CCAGCCCCAA CGTGGCAAAT 
CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG TACGCCCCTG GAGCAGCCGC TAAGATCACG 
TCCGTCTCCA CCGGAAACCT CTGCACTGAG GAGCAGACCC CTCCGCCTCG ACCTGAGGCC 
TACCCCATCC CCACCCAGAC GTACACCAGG GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGCTCCCCC TCAGAACCAG TGGCCCAATT ACGAGGAGAA GCCGCACGTG 
CACACAGATG GTCATCAGTC CGGCCCCGCG ATGCAGCGCG TCACCCGCTC CCAGGAGGAA 
CTTCGGGAGG ATAAAGCTTA CCAACTCGAG CGGCATCGGC TGGAGGCGGT GCTGGATCGC 
AAGTCCGACA GTGACGTGTG GATCCAGCCG AGCTCCTCGG TGGGCTCCAG CACGTCCAGC 
CAGGAGCACC TGGACCCCTG CTCCAAGTCG GGCACCCCCG CCTCCACGCT GACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC 
GCGCAGACAG ACCTGCCCCC GCCACCCCCG CCGCCCCCCG CGCACTACGC CGGAGACTTC 
GACGGGATCC CCGTGGACCT GCCCCTCCCG CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG 
CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGACA GGAAGCGCCG GGAGCAGGAG CGGAAGCTGG GCCAGATGCG CTCCCAGGCC 
CTGAACCCGG CCGCGTTCTC CCCAGTGACC GTCGCGCACA CGAAGCCGGA AAAACCTTCC 
ACGCTCCAGA GGCCCCAGGA CACGGTCATC CGGGAGCTGC AGCCCCAGCA GCAGCCACGC 
ACCATCGAGC GCCGAGACTT GCAGTACATC ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG 
GACAGCCTGT CCCCCGACCC CTGGAAGCGG GACGCCAGGG AGAAGCTGGA GAAGCAGCAG 
CAAATGCACA TCGTGGACAT GCTGAGCAGG GAGATCCGGG AGCTCCAGAG CAAGCCCGAG 
CGCAGCGCCG AGGACAGCGA CCGCCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAAGGACGAG GACGACGAGG AGGAGGAGGA CGACGACGTG 
GACACCATGC TGATCATGCA GCGCCTCGAG GCTGAGCGGA GAGCAAGGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGACCA AACTCTTCCG TGGGATCTTC CGGGGCAGCG 
ATCGGTGCCC GTGACGGCTC CCGGGACCCG AGAGAGAGGC TGGCGAAAAG CCATGATGCA 
GATTTACCTG GAAATTCCGG AGCCCCTGAG AACTTGACAT TCAAAGAGCG CCAGCGGCTG 
TTCTCACAAG GTCAAGATGT GTCCAATAAA GTGAAAGCCT CTCGTAAATT AACAGAACTG 
GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110357.1
CDS	46..5469
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254589.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254589.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAGAAAGAA GATTGGATGT AGATGAGAAA CCCCTTGTTG TGCAGTTGAA TTGGAATAAA 
GACGATCGAG AGGGCAGATT TGTTCTCAAG AATGAGAACG ATGCCCTTCC TCCTAAGAAG 
GCTCAAAGCA ATGGACCTGA AAAGCAGGAG AAAGAAGGTG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCCA AGAAAGAGAA GAAGGAGAAA AAGAAGAGAG AGAAAGAGGC AGTGAGGCAG 
GCATCTGAGA AAGATGATCG GCCTTTCCAA GGGGATGACC TCGAGAATTC CCGACTGGCC 
GCCGAGGTTT ACAAAGACAT GCCCGAGACC AGCTTCACTC GCACGATCTC CAACCCCGAG 
GTGGTCATGA AGCGCCGGCG GCAGCAGAAG CTGGAGAGGC GGATGCAGGA GTTCCGGAGC 
TCGGACGGGC GGCCGGACTC AGGTGGAACA CTGAGAATTT ATGCAGATAG CTTAAAACCG 
AACATTCCCT ACAAGACAAT CCTGCTGTCC ACCACGGATC CTGCTGACTT TGCTGTGGCT 
GAGGCTCTAG AGAAATACGG TCTGGAGAAG GAGAACCCCA AAGATTACTG CATCGCCCGG 
GTTATGCTGC CTCCTGGAGC CCAGCATTCG GATGAAAACG GTGCTAAAGA AACGATCCTC 
GATGATGACG AGTGTCCTTT ACAGATCTTC AGGGAGTGGC CAACTGACCG AGGGGTTTTA 
GTCTTTCAGT TGAAGAGGAG GCCGCCAGAC TACGTCCCGA AGAAAACGAA GAAACATGTC 
GACGGGAAGC TGGTGAAAGG GAAGGAGAGA GCCGAGGGTT CCGGCTATGG CTCAGCACTT 
CCGCCTGAGA AGTTGCCCTA CCTGGTGGAG CTCAGCCCAG TCCCAGGGAG AAGGAGTCAC 
TTTGCCTACT ACAGCTATCA CACTTACGAA GATGGTTCTG ACTCCAGGGA CAAGCCCAAG 
CTTTACCGCC TTCAGTTGAG CGTTACTGAG GTCGGGACAG AGAAGTTCGA GGAGAACTCC 
ATCCAGCTGT TTGGATCAGG AATTCAGCCC CATCACTGTG ACCTCACAAA CATGGATGGA 
GTTGTCACCG TGACTCCGAG AAGCATGGAC GCCGAGACGT ACGTGGAAGG ACAGCGCATC 
TCCGAGACAA CCATGCTGCA GAGTGGGATG AAAGTGCAGT TTGGGGCGTC ACACGTGTTC 
AAGTTTGTGG ACCCCAGCCA GGACCACGCA GTGGCCAAGA GACCTGTGGA TGGAGGCCTG 
ACGGCCAAGG GCCCCAGACA GAAGCCTGGA ATTGTTCAGG AGACAACTTT CGATTTGGGG 
GGAGATGTTC ACAGCGGGGC AGCGCTGCCA GCGAGCAAGA GCGCCACCAG GCTGGACGGC 
GACAGGGCAC TGCCTGCCCC CAGCACGGCC GAGCGCGGGA CAGTGAAGCC GATGCTGAGA 
CTGGAGCAGC AGGACTACCG CCGGCAAGAA AACAGAACTC AGGATGCTCT TGGGCCTGAA 
CTCATACTGC CTGCAAGCAT TGAATTCAGG GAAAGTTCTG AAGATTCATT TTTATCTGCC 
ATTATAAATT ATACTAATAG CTCTACAGTC CATTTTAAGT TGTCACCTAC ATATGTATTA 
TATATGGCAT GTCGGTATGT ATTGTCGAGC CAGTACAGAC CCGATGTCAG TCCTACAGAG 
CGTACTCACA AAGTGATTGC CATAGTCAAC AAGATGGTGA GCATGATGGA GGGGGTGATT 
CAGAAACAGA AGAATATCGC AGGGGCACTT GCTTTCTGGA TGGCCAATGC ATCTGAGCTT 
CTCAACTTCA TTAAGCAGGA CCGAGATCTT AGTCGAATCA CATTGGATGC CCAAGATGTT 
TTAGCACACT TGGTTCAAAT GGCGTTTAAA TATCTGGTTC ATTGTCTTCA GTCAGAACTG 
AATAACTACA TGCCAGCTTT TCTGGATGAC CCTGAAGAGA ACAGTCTACA AAGACCAAAA 
ATAGATGACG TCTTGCACAC GCTCACGGGA GCGATGGCGC TGCTGAGGCG CTGCAGGGTC 
AATGCTGCTC TGACCATCCA GCTTTTCTCT CAGCTCTTCC ACTTCATCAA CATGTGGCTC 
TTCAACAGAT TGGTGACCGA CCCCGAGTCG GGACTGTGCT CTCACTACTG GGGGGCAATC 
ATCCGCCAGC AGCTGGGGCA CGTGGAAGCC TGGGCAGAGA AGCAGGGATT AGAGCTGGCA 
GCCGACTGTC ACCTGAGCCG GATAGTGCAG GCGACCACTT TGCTCACCAT GGACAAATAC 
GCTCCTGAGG ACACCCCAAA TATCAACAGC ACCTGCTTTA AGTTAAATTC GCTGCAGCTG 
CAGGCCTTGC TACAGAATTA CCACTGTGCG CCCGACGAGC CCTTTGTCCC CACGGACCTG 
ATAGAGAATG TCGTGGCCGT GGCCGAAAAC ACGGCCGACG AGCTGGCACG CAGTGATGGG 
CGGGAGGTGC AGCTGGAAGA GGACCCTGAT CTGCAGCTGC CTTTTCTTTT GCCGGAAGAC 
GGCTATTCTT GTGACGTCGT CAGGAACGTT CCAAACGGTT TACAAGAATT TCTGGACCCT 
CTGTGCCAGA GAGGGTTTTG CAGGTTAATT CCTCATGCAC GTTCACCAGG TACTTGGACG 
ATATATTTTG AAGGTGCAGA TTATGAGAGC CATCTTCTGC GTGAGAACAC AGAGCTGGCT 
CAGCCTCTGC GGAAAGAACC TGAAATAATC ACTGTGACTC TCAAAAAGCA AAACGGAATG 
GGCCTCAGCA TTGTCGCAGC AAAGGGTGCT GGTCAAGATA AACTTGGAAT CTATGTCAAG 
TCTGTCGTAA AAGGAGGTGC TGCAGATGTG GATGGACGCC TAGCCGCAGG TGACCAGCTC 
CTCAGTGTGG ATGGACGAAG TCTGGTAGGA CTCTCTCAAG AAAGGGCAGC AGAACTCATG 
ACAAGGACCA GTTCCGTGGT GACACTGGAA GTAGCCAAGC AAGGAGCGAT CTACCACGGC 
CTGGCCACCC TGCTCAACCA GCCGTCCCCG ATGATGCAGA GAGTTTCAGA CCGTCGTGGC 
TCAGGTAAAC CCCGACCAAA GAGCGAAGGT TTCGAGCTCT ATAACAATTC AGCTCAAAAC 
GGGTCACCCG AGAGCCCTCA GCTGCCTTGG GCAGAATACA GCGAACCGAA GAAGCTCCCT 
GGCGATGACA GGCTGATGAA GAACCGGGCT GACCACCGCT CCAGCCCCAA CGTGGCAAAT 
CAGCCCCCCA GTCCCGGAGG GAAAAGCGCG TACGCCCCTG GAGCAGCCGC TAAGATCACG 
TCCGTCTCCA CCGGAAACCT CTGCACTGAG GAGCAGACCC CTCCGCCTCG ACCTGAGGCC 
TACCCCATCC CCACCCAGAC GTACACCAGG GAGTACTTCA CCTTCCCGGC CTCCAGGTCC 
CAGGACCGCG TGGCTCCCCC TCAGAACCAG TGGCCCAATT ACGAGGAGAA GCCGCACGTG 
CACACAGATG GTCATCAGTC CGGCCCCGCG ATGCAGCGCG TCACCCGCTC CCAGGAGGAA 
CTTCGGGAGG ATAAAGCTTA CCAACTCGAG CGGCATCGGC TGGAGGCGGT GCTGGATCGC 
AAGTCCGACA GTGACGTGTG GATCCAGCCG AGCTCCTCGG TGGGCTCCAG CACGTCCAGC 
CAGGAGCACC TGGACCCCTG CTCCAAGTCG GGCACCCCCG CCTCCACGCT GACCAAGAGC 
GGCCCCGGGC GCTGGAAGAC GCCAGCCTCC GCGCAGCCCC TGCCGGCGGC CCCCCCGCCC 
GCGCAGACAG ACCTGCCCCC GCCACCCCCG CCGCCCCCCG CGCACTACGC CGGAGACTTC 
GACGGGATCC CCGTGGACCT GCCCCTCCCG CCCCCTCCCA GCGGCCAGGC GGCGGCGGAG 
CGGAGGAAAC GGGAGGAGCA GCAGCGCTGG TACGAGAAGG AGAAGGCCCG CCTGGAGGAG 
GAGCGGGACA GGAAGCGCCG GGAGCAGGAG CGGAAGCTGG GCCAGATGCG CTCCCAGGCC 
CTGAACCCGG CCGCGTTCTC CCCAGTGACC GTCGCGCACA CGAAGCCGGA AAAACCTTCC 
ACGCTCCAGA GGCCCCAGGA CACGGTCATC CGGGAGCTGC AGCCCCAGCA GCAGCCACGC 
ACCATCGAGC GCCGAGACTT GCAGTACATC ACGGTCAGCA AAGAGGAGCT GTCCTCGGGG 
GACAGCCTGT CCCCCGACCC CTGGAAGCGG GACGCCAGGG AGAAGCTGGA GAAGCAGCAG 
CAAATGCACA TCGTGGACAT GCTGAGCAGG GAGATCCGGG AGCTCCAGAG CAAGCCCGAG 
CGCAGCGCCG AGGACAGCGA CCGCCTGCGC AAGCTCATGC TGGAGTGGCA GTTCCAGAAG 
AGACTGCAGG AGTCCAAGCA GAAGGACGAG GACGACGAGG AGGAGGAGGA CGACGACGTG 
GACACCATGC TGATCATGCA GCGCCTCGAG GCTGAGCGGA GAGCAAGGGA CGAGGAGCGG 
CGGCGGCAGC GGCAGCTGGA CGACATGCGC AGGCGGGAAG CGGAGGACCG CGCCAGGCAG 
GAGGAGGAGA GGACGAAGCG AGACGCCGAA GACAAGAGGC GACAGGAAGA AGGTTATTAC 
AGCCGCCTGG AAGCCGAGAG GCGCAGACAG CACGCGGAGG CCGAGCGCAG GCTGCTGGAG 
CCGGAGGAGC CGGGCCTGGG CAGACCGCCG CTGCCGCGGG GCTACGAGCT CCCGGCCCCG 
TCCTCCCCGG CGGCTGCTCC TCCTCCCCCA CCTCAGCGGA CCGCCTCCTC CCTGCAGGCC 
CCGGCCCTGT CCCCAGACTC CCTGTACACC GCCAGGCTCG TTGCGTACCA CGAGGAGGAG 
GAGGAGGAGG ACTGCAGTCT GGCAGGACCA AACTCTTCCG TGGGATCTTC CGGGGCAGCG 
ATCGGTGCCC GTGACGGCTC CCGGGACCCG AGAGAGAGGC TGGCGAAAAG CCATGATGCA 
GATTTACCTG GAAATTCCGG AGCCCCTGAG AACTTGACAT TCAAAGAGCG CCAGCGGCTG 
TTCTCACAAG GTCAAGATGT GTCCAATAAA GTGAAAGCCT CTCGTAAATT AACAGAACTG 
GAAAACGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254590.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110358.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OFb76149
Clone ID Related Accession (Same CDS sequence)	XM_023254590.1
Accession Version	XM_023254590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC CGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACGTA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCGATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGTG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCGCC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAAAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGA CCAAACTCTT CCGTGGGATC TTCCGGGGCA 
GCGATCGGTG CCCGTGACGG CTCCCGGGAC CCGAGAGAGA GGCTGGCGAA AAGCCATGAT 
GCAGATTTAC CTGGAAATTC CGGAGCCCCT GAGAACTTGA CATTCAAAGA GCGCCAGCGG 
CTGTTCTCAC AAGGTCAAGA TGTGTCCAAT AAAGTGAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110358.1
CDS	46..5472
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGLGRPPLPRGYELPAPSSPAAAPPPPPQRTASSLQAPALSPDSLYTARLVAYHEEEEEEDCSLAGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254590.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254590.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC CGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACGTA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCGATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGTG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCGCC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAAAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGTTACA GGACGAGGAG 
CGGCGGCGGC AGCGGCAGCT GGACGACATG CGCAGGCGGG AAGCGGAGGA CCGCGCCAGG 
CAGGAGGAGG AGAGGACGAA GCGAGACGCC GAAGACAAGA GGCGACAGGA AGAAGGTTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGCGG AGGCCGAGCG CAGGCTGCTG 
GAGCCGGAGG AGCCGGGCCT GGGCAGACCG CCGCTGCCGC GGGGCTACGA GCTCCCGGCC 
CCGTCCTCCC CGGCGGCTGC TCCTCCTCCC CCACCTCAGC GGACCGCCTC CTCCCTGCAG 
GCCCCGGCCC TGTCCCCAGA CTCCCTGTAC ACCGCCAGGC TCGTTGCGTA CCACGAGGAG 
GAGGAGGAGG AGGACTGCAG TCTGGCAGGA CCAAACTCTT CCGTGGGATC TTCCGGGGCA 
GCGATCGGTG CCCGTGACGG CTCCCGGGAC CCGAGAGAGA GGCTGGCGAA AAGCCATGAT 
GCAGATTTAC CTGGAAATTC CGGAGCCCCT GAGAACTTGA CATTCAAAGA GCGCCAGCGG 
CTGTTCTCAC AAGGTCAAGA TGTGTCCAAT AAAGTGAAAG CCTCTCGTAA ATTAACAGAA 
CTGGAAAACG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254591.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110359.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OFb76150
Clone ID Related Accession (Same CDS sequence)	XM_023254591.1
Accession Version	XM_023254591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTTGCTGT GGCTGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGATGGTT CTGACTCCAG GGACAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTCGGGA CAGAGAAGTT CGAGGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGAGTTGTCA CCGTGACTCC GAGAAGCATG GACGCCGAGA CGTACGTGGA AGGACAGCGC 
ATCTCCGAGA CAACCATGCT GCAGAGTGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCAGTGGCCA AGAGACCTGT GGATGGAGGC 
CTGACGGCCA AGGGCCCCAG ACAGAAGCCT GGAATTGTTC AGGAGACAAC TTTCGATTTG 
GGGGGAGATG TTCACAGCGG GGCAGCGCTG CCAGCGAGCA AGAGCGCCAC CAGGCTGGAC 
GGCGACAGGG CACTGCCTGC CCCCAGCACG GCCGAGCGCG GGACAGTGAA GCCGATGCTG 
AGACTGGAGC AGCAGGACTA CCGCCGGCAA GAAAACAGAA CTCAGGATGC TCTTGGGCCT 
GAACTCATAC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC ATTTTTATCT 
GCCATTATAA ATTATACTAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTA 
TTATATATGG CATGTCGGTA TGTATTGTCG AGCCAGTACA GACCCGATGT CAGTCCTACA 
GAGCGTACTC ACAAAGTGAT TGCCATAGTC AACAAGATGG TGAGCATGAT GGAGGGGGTG 
ATTCAGAAAC AGAAGAATAT CGCAGGGGCA CTTGCTTTCT GGATGGCCAA TGCATCTGAG 
CTTCTCAACT TCATTAAGCA GGACCGAGAT CTTAGTCGAA TCACATTGGA TGCCCAAGAT 
GTTTTAGCAC ACTTGGTTCA AATGGCGTTT AAATATCTGG TTCATTGTCT TCAGTCAGAA 
CTGAATAACT ACATGCCAGC TTTTCTGGAT GACCCTGAAG AGAACAGTCT ACAAAGACCA 
AAAATAGATG ACGTCTTGCA CACGCTCACG GGAGCGATGG CGCTGCTGAG GCGCTGCAGG 
GTCAATGCTG CTCTGACCAT CCAGCTTTTC TCTCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA GATTGGTGAC CGACCCCGAG TCGGGACTGT GCTCTCACTA CTGGGGGGCA 
ATCATCCGCC AGCAGCTGGG GCACGTGGAA GCCTGGGCAG AGAAGCAGGG ATTAGAGCTG 
GCAGCCGACT GTCACCTGAG CCGGATAGTG CAGGCGACCA CTTTGCTCAC CATGGACAAA 
TACGCTCCTG AGGACACCCC AAATATCAAC AGCACCTGCT TTAAGTTAAA TTCGCTGCAG 
CTGCAGGCCT TGCTACAGAA TTACCACTGT GCGCCCGACG AGCCCTTTGT CCCCACGGAC 
CTGATAGAGA ATGTCGTGGC CGTGGCCGAA AACACGGCCG ACGAGCTGGC ACGCAGTGAT 
GGGCGGGAGG TGCAGCTGGA AGAGGACCCT GATCTGCAGC TGCCTTTTCT TTTGCCGGAA 
GACGGCTATT CTTGTGACGT CGTCAGGAAC GTTCCAAACG GTTTACAAGA ATTTCTGGAC 
CCTCTGTGCC AGAGAGGGTT TTGCAGGTTA ATTCCTCATG CACGTTCACC AGGTACTTGG 
ACGATATATT TTGAAGGTGC AGATTATGAG AGCCATCTTC TGCGTGAGAA CACAGAGCTG 
GCTCAGCCTC TGCGGAAAGA ACCTGAAATA ATCACTGTGA CTCTCAAAAA GCAAAACGGA 
ATGGGCCTCA GCATTGTCGC AGCAAAGGGT GCTGGTCAAG ATAAACTTGG AATCTATGTC 
AAGTCTGTCG TAAAAGGAGG TGCTGCAGAT GTGGATGGAC GCCTAGCCGC AGGTGACCAG 
CTCCTCAGTG TGGATGGACG AAGTCTGGTA GGACTCTCTC AAGAAAGGGC AGCAGAACTC 
ATGACAAGGA CCAGTTCCGT GGTGACACTG GAAGTAGCCA AGCAAGGAGC GATCTACCAC 
GGCCTGGCCA CCCTGCTCAA CCAGCCGTCC CCGATGATGC AGAGAGTTTC AGACCGTCGT 
GGCTCAGGTA AACCCCGACC AAAGAGCGAA GGTTTCGAGC TCTATAACAA TTCAGCTCAA 
AACGGGTCAC CCGAGAGCCC TCAGCTGCCT TGGGCAGAAT ACAGCGAACC GAAGAAGCTC 
CCTGGCGATG ACAGGCTGAT GAAGAACCGG GCTGACCACC GCTCCAGCCC CAACGTGGCA 
AATCAGCCCC CCAGTCCCGG AGGGAAAAGC GCGTACGCCC CTGGAGCAGC CGCTAAGATC 
ACGTCCGTCT CCACCGGAAA CCTCTGCACT GAGGAGCAGA CCCCTCCGCC TCGACCTGAG 
GCCTACCCCA TCCCCACCCA GACGTACACC AGGGAGTACT TCACCTTCCC GGCCTCCAGG 
TCCCAGGACC GCGTGGCTCC CCCTCAGAAC CAGTGGCCCA ATTACGAGGA GAAGCCGCAC 
GTGCACACAG ATGGTCATCA GTCCGGCCCC GCGATGCAGC GCGTCACCCG CTCCCAGGAG 
GAACTTCGGG AGGATAAAGC TTACCAACTC GAGCGGCATC GGCTGGAGGC GGTGCTGGAT 
CGCAAGTCCG ACAGTGACGT GTGGATCCAG CCGAGCTCCT CGGTGGGCTC CAGCACGTCC 
AGCCAGGAGC ACCTGGACCC CTGCTCCAAG TCGGGCACCC CCGCCTCCAC GCTGACCAAG 
AGCGGCCCCG GGCGCTGGAA GACGCCAGCC TCCGCGCAGC CCCTGCCGGC GGCCCCCCCG 
CCCGCGCAGA CAGACCTGCC CCCGCCACCC CCGCCGCCCC CCGCGCACTA CGCCGGAGAC 
TTCGACGGGA TCCCCGTGGA CCTGCCCCTC CCGCCCCCTC CCAGCGGCCA GGCGGCGGCG 
GAGCGGAGGA AACGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG ACAGGAAGCG CCGGGAGCAG GAGCGGAAGC TGGGCCAGAT GCGCTCCCAG 
GCCCTGAACC CGGCCGCGTT CTCCCCAGTG ACCGTCGCGC ACACGAAGCC GGAAAAACCT 
TCCACGCTCC AGAGGCCCCA GGACACGGTC ATCCGGGAGC TGCAGCCCCA GCAGCAGCCA 
CGCACCATCG AGCGCCGAGA CTTGCAGTAC ATCACGGTCA GCAAAGAGGA GCTGTCCTCG 
GGGGACAGCC TGTCCCCCGA CCCCTGGAAG CGGGACGCCA GGGAGAAGCT GGAGAAGCAG 
CAGCAAATGC ACATCGTGGA CATGCTGAGC AGGGAGATCC GGGAGCTCCA GAGCAAGCCC 
GAGCGCAGCG CCGAGGACAG CGACCGCCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGACGAC 
GTGGACACCA TGCTGATCAT GCAGCGCCTC GAGGCTGAGC GGAGAGCAAG GTTACAGGAC 
GAGGAGCGGC GGCGGCAGCG GCAGCTGGAC GACATGCGCA GGCGGGAAGC GGAGGACCGC 
GCCAGGCAGG AGGAGGAGAG GACGAAGCGA GACGCCGAAG ACAAGAGGCG ACAGGAAGAA 
GGTTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGCGGAGGC CGAGCGCAGG 
CTGCTGGAGC CGGAGGAGCC GGGACCAAAC TCTTCCGTGG GATCTTCCGG GGCAGCGATC 
GGTGCCCGTG ACGGCTCCCG GGACCCGAGA GAGAGGCTGG CGAAAAGCCA TGATGCAGAT 
TTACCTGGAA ATTCCGGAGC CCCTGAGAAC TTGACATTCA AAGAGCGCCA GCGGCTGTTC 
TCACAAGGTC AAGATGTGTC CAATAAAGTG AAAGCCTCTC GTAAATTAAC AGAACTGGAA 
AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110359.1
CDS	46..5226
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKRRQEEGYYSRLEAERRRQHAEAERRLLEPEEPGPNSSVGSSGAAIGARDGSRDPRERLAKSHDADLPGNSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_023254591.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254591.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGGCTCAA 
AGCAATGGAC CTGAAAAGCA GGAGAAAGAA GGTGTTATCC AGAACTTCAA GAGAACTCTC 
TCCAAGAAAG AGAAGAAGGA GAAAAAGAAG AGAGAGAAAG AGGCAGTGAG GCAGGCATCT 
GAGAAAGATG ATCGGCCTTT CCAAGGGGAT GACCTCGAGA ATTCCCGACT GGCCGCCGAG 
GTTTACAAAG ACATGCCCGA GACCAGCTTC ACTCGCACGA TCTCCAACCC CGAGGTGGTC 
ATGAAGCGCC GGCGGCAGCA GAAGCTGGAG AGGCGGATGC AGGAGTTCCG GAGCTCGGAC 
GGGCGGCCGG ACTCAGGTGG AACACTGAGA ATTTATGCAG ATAGCTTAAA ACCGAACATT 
CCCTACAAGA CAATCCTGCT GTCCACCACG GATCCTGCTG ACTTTGCTGT GGCTGAGGCT 
CTAGAGAAAT ACGGTCTGGA GAAGGAGAAC CCCAAAGATT ACTGCATCGC CCGGGTTATG 
CTGCCTCCTG GAGCCCAGCA TTCGGATGAA AACGGTGCTA AAGAAACGAT CCTCGATGAT 
GACGAGTGTC CTTTACAGAT CTTCAGGGAG TGGCCAACTG ACCGAGGGGT TTTAGTCTTT 
CAGTTGAAGA GGAGGCCGCC AGACTACGTC CCGAAGAAAA CGAAGAAACA TGTCGACGGG 
AAGCTGGTGA AAGGGAAGGA GAGAGCCGAG GGTTCCGGCT ATGGCTCAGC ACTTCCGCCT 
GAGAAGTTGC CCTACCTGGT GGAGCTCAGC CCAGATGGTT CTGACTCCAG GGACAAGCCC 
AAGCTTTACC GCCTTCAGTT GAGCGTTACT GAGGTCGGGA CAGAGAAGTT CGAGGAGAAC 
TCCATCCAGC TGTTTGGATC AGGAATTCAG CCCCATCACT GTGACCTCAC AAACATGGAT 
GGAGTTGTCA CCGTGACTCC GAGAAGCATG GACGCCGAGA CGTACGTGGA AGGACAGCGC 
ATCTCCGAGA CAACCATGCT GCAGAGTGGG ATGAAAGTGC AGTTTGGGGC GTCACACGTG 
TTCAAGTTTG TGGACCCCAG CCAGGACCAC GCAGTGGCCA AGAGACCTGT GGATGGAGGC 
CTGACGGCCA AGGGCCCCAG ACAGAAGCCT GGAATTGTTC AGGAGACAAC TTTCGATTTG 
GGGGGAGATG TTCACAGCGG GGCAGCGCTG CCAGCGAGCA AGAGCGCCAC CAGGCTGGAC 
GGCGACAGGG CACTGCCTGC CCCCAGCACG GCCGAGCGCG GGACAGTGAA GCCGATGCTG 
AGACTGGAGC AGCAGGACTA CCGCCGGCAA GAAAACAGAA CTCAGGATGC TCTTGGGCCT 
GAACTCATAC TGCCTGCAAG CATTGAATTC AGGGAAAGTT CTGAAGATTC ATTTTTATCT 
GCCATTATAA ATTATACTAA TAGCTCTACA GTCCATTTTA AGTTGTCACC TACATATGTA 
TTATATATGG CATGTCGGTA TGTATTGTCG AGCCAGTACA GACCCGATGT CAGTCCTACA 
GAGCGTACTC ACAAAGTGAT TGCCATAGTC AACAAGATGG TGAGCATGAT GGAGGGGGTG 
ATTCAGAAAC AGAAGAATAT CGCAGGGGCA CTTGCTTTCT GGATGGCCAA TGCATCTGAG 
CTTCTCAACT TCATTAAGCA GGACCGAGAT CTTAGTCGAA TCACATTGGA TGCCCAAGAT 
GTTTTAGCAC ACTTGGTTCA AATGGCGTTT AAATATCTGG TTCATTGTCT TCAGTCAGAA 
CTGAATAACT ACATGCCAGC TTTTCTGGAT GACCCTGAAG AGAACAGTCT ACAAAGACCA 
AAAATAGATG ACGTCTTGCA CACGCTCACG GGAGCGATGG CGCTGCTGAG GCGCTGCAGG 
GTCAATGCTG CTCTGACCAT CCAGCTTTTC TCTCAGCTCT TCCACTTCAT CAACATGTGG 
CTCTTCAACA GATTGGTGAC CGACCCCGAG TCGGGACTGT GCTCTCACTA CTGGGGGGCA 
ATCATCCGCC AGCAGCTGGG GCACGTGGAA GCCTGGGCAG AGAAGCAGGG ATTAGAGCTG 
GCAGCCGACT GTCACCTGAG CCGGATAGTG CAGGCGACCA CTTTGCTCAC CATGGACAAA 
TACGCTCCTG AGGACACCCC AAATATCAAC AGCACCTGCT TTAAGTTAAA TTCGCTGCAG 
CTGCAGGCCT TGCTACAGAA TTACCACTGT GCGCCCGACG AGCCCTTTGT CCCCACGGAC 
CTGATAGAGA ATGTCGTGGC CGTGGCCGAA AACACGGCCG ACGAGCTGGC ACGCAGTGAT 
GGGCGGGAGG TGCAGCTGGA AGAGGACCCT GATCTGCAGC TGCCTTTTCT TTTGCCGGAA 
GACGGCTATT CTTGTGACGT CGTCAGGAAC GTTCCAAACG GTTTACAAGA ATTTCTGGAC 
CCTCTGTGCC AGAGAGGGTT TTGCAGGTTA ATTCCTCATG CACGTTCACC AGGTACTTGG 
ACGATATATT TTGAAGGTGC AGATTATGAG AGCCATCTTC TGCGTGAGAA CACAGAGCTG 
GCTCAGCCTC TGCGGAAAGA ACCTGAAATA ATCACTGTGA CTCTCAAAAA GCAAAACGGA 
ATGGGCCTCA GCATTGTCGC AGCAAAGGGT GCTGGTCAAG ATAAACTTGG AATCTATGTC 
AAGTCTGTCG TAAAAGGAGG TGCTGCAGAT GTGGATGGAC GCCTAGCCGC AGGTGACCAG 
CTCCTCAGTG TGGATGGACG AAGTCTGGTA GGACTCTCTC AAGAAAGGGC AGCAGAACTC 
ATGACAAGGA CCAGTTCCGT GGTGACACTG GAAGTAGCCA AGCAAGGAGC GATCTACCAC 
GGCCTGGCCA CCCTGCTCAA CCAGCCGTCC CCGATGATGC AGAGAGTTTC AGACCGTCGT 
GGCTCAGGTA AACCCCGACC AAAGAGCGAA GGTTTCGAGC TCTATAACAA TTCAGCTCAA 
AACGGGTCAC CCGAGAGCCC TCAGCTGCCT TGGGCAGAAT ACAGCGAACC GAAGAAGCTC 
CCTGGCGATG ACAGGCTGAT GAAGAACCGG GCTGACCACC GCTCCAGCCC CAACGTGGCA 
AATCAGCCCC CCAGTCCCGG AGGGAAAAGC GCGTACGCCC CTGGAGCAGC CGCTAAGATC 
ACGTCCGTCT CCACCGGAAA CCTCTGCACT GAGGAGCAGA CCCCTCCGCC TCGACCTGAG 
GCCTACCCCA TCCCCACCCA GACGTACACC AGGGAGTACT TCACCTTCCC GGCCTCCAGG 
TCCCAGGACC GCGTGGCTCC CCCTCAGAAC CAGTGGCCCA ATTACGAGGA GAAGCCGCAC 
GTGCACACAG ATGGTCATCA GTCCGGCCCC GCGATGCAGC GCGTCACCCG CTCCCAGGAG 
GAACTTCGGG AGGATAAAGC TTACCAACTC GAGCGGCATC GGCTGGAGGC GGTGCTGGAT 
CGCAAGTCCG ACAGTGACGT GTGGATCCAG CCGAGCTCCT CGGTGGGCTC CAGCACGTCC 
AGCCAGGAGC ACCTGGACCC CTGCTCCAAG TCGGGCACCC CCGCCTCCAC GCTGACCAAG 
AGCGGCCCCG GGCGCTGGAA GACGCCAGCC TCCGCGCAGC CCCTGCCGGC GGCCCCCCCG 
CCCGCGCAGA CAGACCTGCC CCCGCCACCC CCGCCGCCCC CCGCGCACTA CGCCGGAGAC 
TTCGACGGGA TCCCCGTGGA CCTGCCCCTC CCGCCCCCTC CCAGCGGCCA GGCGGCGGCG 
GAGCGGAGGA AACGGGAGGA GCAGCAGCGC TGGTACGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG ACAGGAAGCG CCGGGAGCAG GAGCGGAAGC TGGGCCAGAT GCGCTCCCAG 
GCCCTGAACC CGGCCGCGTT CTCCCCAGTG ACCGTCGCGC ACACGAAGCC GGAAAAACCT 
TCCACGCTCC AGAGGCCCCA GGACACGGTC ATCCGGGAGC TGCAGCCCCA GCAGCAGCCA 
CGCACCATCG AGCGCCGAGA CTTGCAGTAC ATCACGGTCA GCAAAGAGGA GCTGTCCTCG 
GGGGACAGCC TGTCCCCCGA CCCCTGGAAG CGGGACGCCA GGGAGAAGCT GGAGAAGCAG 
CAGCAAATGC ACATCGTGGA CATGCTGAGC AGGGAGATCC GGGAGCTCCA GAGCAAGCCC 
GAGCGCAGCG CCGAGGACAG CGACCGCCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTGC AGGAGTCCAA GCAGAAGGAC GAGGACGACG AGGAGGAGGA GGACGACGAC 
GTGGACACCA TGCTGATCAT GCAGCGCCTC GAGGCTGAGC GGAGAGCAAG GTTACAGGAC 
GAGGAGCGGC GGCGGCAGCG GCAGCTGGAC GACATGCGCA GGCGGGAAGC GGAGGACCGC 
GCCAGGCAGG AGGAGGAGAG GACGAAGCGA GACGCCGAAG ACAAGAGGCG ACAGGAAGAA 
GGTTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGCGGAGGC CGAGCGCAGG 
CTGCTGGAGC CGGAGGAGCC GGGACCAAAC TCTTCCGTGG GATCTTCCGG GGCAGCGATC 
GGTGCCCGTG ACGGCTCCCG GGACCCGAGA GAGAGGCTGG CGAAAAGCCA TGATGCAGAT 
TTACCTGGAA ATTCCGGAGC CCCTGAGAAC TTGACATTCA AAGAGCGCCA GCGGCTGTTC 
TCACAAGGTC AAGATGTGTC CAATAAAGTG AAAGCCTCTC GTAAATTAAC AGAACTGGAA 
AACGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_023254592.1 ) cDNA ORF clone, Felis catus(Domestic cat) -> XP_023110360.1 Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

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CloneID	OFb76151
Clone ID Related Accession (Same CDS sequence)	XM_023254592.1
Accession Version	XM_023254592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1512921600000
Organism	Felis catus(domestic cat)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018727.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Felis catus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC CGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACGTA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCGATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGTG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCGCC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAAAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGGTCATGG TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023110360.1
CDS	46..4953
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDVDEKPLVVQLNWNKDDREGRFVLKNENDALPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEAVRQASEKDDRPFQGDDLENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLERRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDENGAKETILDDDECPLQIFREWPTDRGVLVFQLKRRPPDYVPKKTKKHVDGKLVKGKERAEGSGYGSALPPEKLPYLVELSPVPGRRSHFAYYSYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKFEENSIQLFGSGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHAVAKRPVDGGLTAKGPRQKPGIVQETTFDLGGDVHSGAALPASKSATRLDGDRALPAPSTAERGTVKPMLRLEQQDYRRQENRTQDALGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSSQYRPDVSPTERTHKVIAIVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMALLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPESGLCSHYWGAIIRQQLGHVEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPEDTPNINSTCFKLNSLQLQALLQNYHCAPDEPFVPTDLIENVVAVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNVPNGLQEFLDPLCQRGFCRLIPHARSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRVSDRRGSGKPRPKSEGFELYNNSAQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYAPGAAAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASRSQDRVAPPQNQWPNYEEKPHVHTDGHQSGPAMQRVTRSQEELREDKAYQLERHRLEAVLDRKSDSDVWIQPSSSVGSSTSSQEHLDPCSKSGTPASTLTKSGPGRWKTPASAQPLPAAPPPAQTDLPPPPPPPPAHYAGDFDGIPVDLPLPPPPSGQAAAERRKREEQQRWYEKEKARLEEERDRKRREQERKLGQMRSQALNPAAFSPVTVAHTKPEKPSTLQRPQDTVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAREKLEKQQQMHIVDMLSREIRELQSKPERSAEDSDRLRKLMLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARDEERRRQRQLDDMRRREAEDRARQEEERTKRDAEDKVMVL

Target ORF information:

RefSeq Version	XM_023254592.1
Organism	Felis catus(domestic cat)
Definition	Felis catus afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023254592.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGCGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCCA CCGAGGATTT GGAGTTTCAT 
GGCGTAATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGCATT 
CGGGTCTCTA GTACGGCCAC CACGCAAGAC GTGATCGAGA CGCTGGCAGA GAAATTCCGC 
CCTGACATGC GGATGCTGTC GTCCCCCAAG TACTCCCTCT ACGAAGTCCA CGTCAGCGGA 
GAAAGAAGAT TGGATGTAGA TGAGAAACCC CTTGTTGTGC AGTTGAATTG GAATAAAGAC 
GATCGAGAGG GCAGATTTGT TCTCAAGAAT GAGAACGATG CCCTTCCTCC TAAGAAGGCT 
CAAAGCAATG GACCTGAAAA GCAGGAGAAA GAAGGTGTTA TCCAGAACTT CAAGAGAACT 
CTCTCCAAGA AAGAGAAGAA GGAGAAAAAG AAGAGAGAGA AAGAGGCAGT GAGGCAGGCA 
TCTGAGAAAG ATGATCGGCC TTTCCAAGGG GATGACCTCG AGAATTCCCG ACTGGCCGCC 
GAGGTTTACA AAGACATGCC CGAGACCAGC TTCACTCGCA CGATCTCCAA CCCCGAGGTG 
GTCATGAAGC GCCGGCGGCA GCAGAAGCTG GAGAGGCGGA TGCAGGAGTT CCGGAGCTCG 
GACGGGCGGC CGGACTCAGG TGGAACACTG AGAATTTATG CAGATAGCTT AAAACCGAAC 
ATTCCCTACA AGACAATCCT GCTGTCCACC ACGGATCCTG CTGACTTTGC TGTGGCTGAG 
GCTCTAGAGA AATACGGTCT GGAGAAGGAG AACCCCAAAG ATTACTGCAT CGCCCGGGTT 
ATGCTGCCTC CTGGAGCCCA GCATTCGGAT GAAAACGGTG CTAAAGAAAC GATCCTCGAT 
GATGACGAGT GTCCTTTACA GATCTTCAGG GAGTGGCCAA CTGACCGAGG GGTTTTAGTC 
TTTCAGTTGA AGAGGAGGCC GCCAGACTAC GTCCCGAAGA AAACGAAGAA ACATGTCGAC 
GGGAAGCTGG TGAAAGGGAA GGAGAGAGCC GAGGGTTCCG GCTATGGCTC AGCACTTCCG 
CCTGAGAAGT TGCCCTACCT GGTGGAGCTC AGCCCAGTCC CAGGGAGAAG GAGTCACTTT 
GCCTACTACA GCTATCACAC TTACGAAGAT GGTTCTGACT CCAGGGACAA GCCCAAGCTT 
TACCGCCTTC AGTTGAGCGT TACTGAGGTC GGGACAGAGA AGTTCGAGGA GAACTCCATC 
CAGCTGTTTG GATCAGGAAT TCAGCCCCAT CACTGTGACC TCACAAACAT GGATGGAGTT 
GTCACCGTGA CTCCGAGAAG CATGGACGCC GAGACGTACG TGGAAGGACA GCGCATCTCC 
GAGACAACCA TGCTGCAGAG TGGGATGAAA GTGCAGTTTG GGGCGTCACA CGTGTTCAAG 
TTTGTGGACC CCAGCCAGGA CCACGCAGTG GCCAAGAGAC CTGTGGATGG AGGCCTGACG 
GCCAAGGGCC CCAGACAGAA GCCTGGAATT GTTCAGGAGA CAACTTTCGA TTTGGGGGGA 
GATGTTCACA GCGGGGCAGC GCTGCCAGCG AGCAAGAGCG CCACCAGGCT GGACGGCGAC 
AGGGCACTGC CTGCCCCCAG CACGGCCGAG CGCGGGACAG TGAAGCCGAT GCTGAGACTG 
GAGCAGCAGG ACTACCGCCG GCAAGAAAAC AGAACTCAGG ATGCTCTTGG GCCTGAACTC 
ATACTGCCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT ATCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAT TTTAAGTTGT CACCTACATA TGTATTATAT 
ATGGCATGTC GGTATGTATT GTCGAGCCAG TACAGACCCG ATGTCAGTCC TACAGAGCGT 
ACTCACAAAG TGATTGCCAT AGTCAACAAG ATGGTGAGCA TGATGGAGGG GGTGATTCAG 
AAACAGAAGA ATATCGCAGG GGCACTTGCT TTCTGGATGG CCAATGCATC TGAGCTTCTC 
AACTTCATTA AGCAGGACCG AGATCTTAGT CGAATCACAT TGGATGCCCA AGATGTTTTA 
GCACACTTGG TTCAAATGGC GTTTAAATAT CTGGTTCATT GTCTTCAGTC AGAACTGAAT 
AACTACATGC CAGCTTTTCT GGATGACCCT GAAGAGAACA GTCTACAAAG ACCAAAAATA 
GATGACGTCT TGCACACGCT CACGGGAGCG ATGGCGCTGC TGAGGCGCTG CAGGGTCAAT 
GCTGCTCTGA CCATCCAGCT TTTCTCTCAG CTCTTCCACT TCATCAACAT GTGGCTCTTC 
AACAGATTGG TGACCGACCC CGAGTCGGGA CTGTGCTCTC ACTACTGGGG GGCAATCATC 
CGCCAGCAGC TGGGGCACGT GGAAGCCTGG GCAGAGAAGC AGGGATTAGA GCTGGCAGCC 
GACTGTCACC TGAGCCGGAT AGTGCAGGCG ACCACTTTGC TCACCATGGA CAAATACGCT 
CCTGAGGACA CCCCAAATAT CAACAGCACC TGCTTTAAGT TAAATTCGCT GCAGCTGCAG 
GCCTTGCTAC AGAATTACCA CTGTGCGCCC GACGAGCCCT TTGTCCCCAC GGACCTGATA 
GAGAATGTCG TGGCCGTGGC CGAAAACACG GCCGACGAGC TGGCACGCAG TGATGGGCGG 
GAGGTGCAGC TGGAAGAGGA CCCTGATCTG CAGCTGCCTT TTCTTTTGCC GGAAGACGGC 
TATTCTTGTG ACGTCGTCAG GAACGTTCCA AACGGTTTAC AAGAATTTCT GGACCCTCTG 
TGCCAGAGAG GGTTTTGCAG GTTAATTCCT CATGCACGTT CACCAGGTAC TTGGACGATA 
TATTTTGAAG GTGCAGATTA TGAGAGCCAT CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGCGGA AAGAACCTGA AATAATCACT GTGACTCTCA AAAAGCAAAA CGGAATGGGC 
CTCAGCATTG TCGCAGCAAA GGGTGCTGGT CAAGATAAAC TTGGAATCTA TGTCAAGTCT 
GTCGTAAAAG GAGGTGCTGC AGATGTGGAT GGACGCCTAG CCGCAGGTGA CCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTAGGACTC TCTCAAGAAA GGGCAGCAGA ACTCATGACA 
AGGACCAGTT CCGTGGTGAC ACTGGAAGTA GCCAAGCAAG GAGCGATCTA CCACGGCCTG 
GCCACCCTGC TCAACCAGCC GTCCCCGATG ATGCAGAGAG TTTCAGACCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG CGAAGGTTTC GAGCTCTATA ACAATTCAGC TCAAAACGGG 
TCACCCGAGA GCCCTCAGCT GCCTTGGGCA GAATACAGCG AACCGAAGAA GCTCCCTGGC 
GATGACAGGC TGATGAAGAA CCGGGCTGAC CACCGCTCCA GCCCCAACGT GGCAAATCAG 
CCCCCCAGTC CCGGAGGGAA AAGCGCGTAC GCCCCTGGAG CAGCCGCTAA GATCACGTCC 
GTCTCCACCG GAAACCTCTG CACTGAGGAG CAGACCCCTC CGCCTCGACC TGAGGCCTAC 
CCCATCCCCA CCCAGACGTA CACCAGGGAG TACTTCACCT TCCCGGCCTC CAGGTCCCAG 
GACCGCGTGG CTCCCCCTCA GAACCAGTGG CCCAATTACG AGGAGAAGCC GCACGTGCAC 
ACAGATGGTC ATCAGTCCGG CCCCGCGATG CAGCGCGTCA CCCGCTCCCA GGAGGAACTT 
CGGGAGGATA AAGCTTACCA ACTCGAGCGG CATCGGCTGG AGGCGGTGCT GGATCGCAAG 
TCCGACAGTG ACGTGTGGAT CCAGCCGAGC TCCTCGGTGG GCTCCAGCAC GTCCAGCCAG 
GAGCACCTGG ACCCCTGCTC CAAGTCGGGC ACCCCCGCCT CCACGCTGAC CAAGAGCGGC 
CCCGGGCGCT GGAAGACGCC AGCCTCCGCG CAGCCCCTGC CGGCGGCCCC CCCGCCCGCG 
CAGACAGACC TGCCCCCGCC ACCCCCGCCG CCCCCCGCGC ACTACGCCGG AGACTTCGAC 
GGGATCCCCG TGGACCTGCC CCTCCCGCCC CCTCCCAGCG GCCAGGCGGC GGCGGAGCGG 
AGGAAACGGG AGGAGCAGCA GCGCTGGTAC GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG 
CGGGACAGGA AGCGCCGGGA GCAGGAGCGG AAGCTGGGCC AGATGCGCTC CCAGGCCCTG 
AACCCGGCCG CGTTCTCCCC AGTGACCGTC GCGCACACGA AGCCGGAAAA ACCTTCCACG 
CTCCAGAGGC CCCAGGACAC GGTCATCCGG GAGCTGCAGC CCCAGCAGCA GCCACGCACC 
ATCGAGCGCC GAGACTTGCA GTACATCACG GTCAGCAAAG AGGAGCTGTC CTCGGGGGAC 
AGCCTGTCCC CCGACCCCTG GAAGCGGGAC GCCAGGGAGA AGCTGGAGAA GCAGCAGCAA 
ATGCACATCG TGGACATGCT GAGCAGGGAG ATCCGGGAGC TCCAGAGCAA GCCCGAGCGC 
AGCGCCGAGG ACAGCGACCG CCTGCGCAAG CTCATGCTGG AGTGGCAGTT CCAGAAGAGA 
CTGCAGGAGT CCAAGCAGAA GGACGAGGAC GACGAGGAGG AGGAGGACGA CGACGTGGAC 
ACCATGCTGA TCATGCAGCG CCTCGAGGCT GAGCGGAGAG CAAGGGACGA GGAGCGGCGG 
CGGCAGCGGC AGCTGGACGA CATGCGCAGG CGGGAAGCGG AGGACCGCGC CAGGCAGGAG 
GAGGAGAGGA CGAAGCGAGA CGCCGAAGAC AAGGTCATGG TCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AFDN
Entrez Gene ID	101081386
Full Name	afadin, adherens junction formation factor
Gene Type	protein-coding
Organism	Felis catus(Domestic cat)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AFDN cDNA ORF clone, Felis catus(Domestic cat)

Gene

Clones

gRNAs

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