Dpse\GA29217 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA29217
Entrez Gene ID	6901423
Full Name	GA29217 gene product from transcript GA29217-RB
Synonyms	GA29217,dpse_GLEANR_978
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_015186190.1	XP_015041676.1	uncharacterized protein, isoform B
XM_002134073.2	XP_002134109.1	uncharacterized protein, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA29217 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA29217 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe13418	XM_002134073.2 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA29217), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODe24819	XM_015186190.1 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA29217), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA29217 ( XM_002134073.2 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_002134109.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA29217), mRNA.

Price & Availability↑

CloneID	ODe13418
Clone ID Related Accession (Same CDS sequence)	XM_002134073.2
Accession Version	XM_002134073.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). On Dec 22, 2015 this sequence version replaced XM_002134073.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCATG CCGTGAAGGC AACGCGCGGC CGCCCGCTCA ATACGCTCCG CTTCGAGAAC 
GATGAGCTCG AGTGCCTCTA TCAGCGGTAC ACGCTCAAGC TCCAGCGCTT CTCGGTGCTC 
GGTGTCGTTG CACTCGTTGT GGTGCTATGC GGAGTGATGG CGGCCCTCTC GCTGGCCTTC 
AACAGTGCGG CCACCTTTCA TAACATCTTC AATTCGATCG TTTGCTTGCT GTTCGCCGTG 
GTGCTGATCC TGTTGCAATG TTCGGTGATC AAGGATCACC ATTTGCCGAC ACTGTGCTAT 
GGGATTCTAC TGTTTACGGC CAGCATTTGT GTGGTGTCGA TGCCGACGCT GGGCAGCGTC 
TTTCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGTGTCT GGCAGATCGT ATTCGTTGTA 
TTTCTGGCCT ACGCCATGAT GCCGCTGCAG ATCTGGGAGG CGGTGGCCTT CGGCATCGCC 
CTGCCCTCGG TGCACATAAG CCTCACCGTC TACAAGATCT TCACCGATGC CCTGCGATAC 
CTGGAGTACA ACCAGCTGAT TGCGAATATT GTTATCTTCA TTGGCGTGAA TGTGGCTGGG 
CTGGTGGTCA ACATTATGAT GGAGCGGGCC CAGCGAAGGA CCTTCCTCGA CACGCGCAAC 
TGTATTGCCT CTCGTCTGGA GATCCAGGAC GAGAACGAGA AGCTCGAGCG GTTGCTGCTC 
TCTGTCCTGC CCCAACATGT GGCCATGCAG ATGAAGAACG ACATCCTATC GCCGGTGGCC 
GGGCAGTTCC ATCGCATCTA CATCCAGAAG CATGAGAATG TCAGCATTCT CTTTGCGGAC 
ATTGTGGGCT TCACGGTCCT CTCGTCGCAG TGCAGTGCTC AGGAGTTGGT GCGATTGCTC 
AACGAGCTCT TTGGGCGATT CGATCAGTTG GCACATGATA ATCATTGCCT GCGCATTAAG 
ATCTTGGGCG ATTGTTATTA TTGTGTTTCG GGTTTGCCAG AGCCCCGCAA GGATCATGCA 
AAATGTGCAG TGGAAATGGG ACTCGATATG ATCGATGCCA TTGCCACTGT CGTTGAAGCC 
ACCGATGTCA TACTCAATAT GCGCGTGGGC ATCCACACTG GCCGCGTGCT GTGCGGTGTT 
TTGGGCCTCC GGAAGTGGCA GTTCGATGTG TGGTCCAACG ATGTGACCCT GGCGAATCAT 
ATGGAATCGG GCGGTGAGCC GGGTCGAGTG CATGTCACTC GCGCCACCCT CGATTCGCTG 
TCGGGCGAGT ACGAAGTGGA GGCAGGACAC GGCGACGAGC GATCCTCGTA TCTGCGCGAT 
CATGGAGTGG ATACGTTCTT TATAGTGCCA CCACCGCACC GTCGCAAGCC CCTAATGCTC 
AACACTCTGG GCGTACGCTC GGCGATCGGT AGCAGGCGGA AGCTGTCATT CCGGAATGTC 
TCCAATGTGG TCATGCAACT GTTGCACACG ATCAAGTTCT CGGAGCCAGT GCCGTTCTCG 
AATATTGCCA CCGGTTCGTT TCCTTCGGCG GCCAATGCAT TGGTGGGCGG TGCCGGTGTG 
GGTCTGGGCG TTGGTCTGAG CGTGGCACGT GGCAGCACCT GTGAGGCAAG CGGCGGCGGC 
GTCAGCGTCG GCGGCGGAGG CGTCGGCAGT GTACAGGCCG TATCGGAGAA GGGTTCACGC 
AAGGATGCGG TAATACGTCT GCAGAAAATC TTGCATGCCA CCCCGCCACC GCACGCTCAT 
GGCATGCCAC CCATGGGCTA TGGTAGCGTC GTCGGCAGTG GTGGTGGTGG TGGTGCTGGT 
GGTGGTGTCG GTATTGACGG TGGCAGTGTG GGCTGTGGTA TTGGCAGTGG CTGCGGTGGG 
GGTGGCAGTG TCCAAGTGAC CGTAGGCACA GATCCCAACT CAACAGCGAG TACGATTAGT 
CGCATTCATA GGCACAATCA CAAGAACAAT AAATCACAGT CCAAGGTGGC CGACAAATTC 
AAGAGACCCT TCCGCAAACG GCATTCGGTG GCAGCGCATC ATCAGCCCAC GAATCGTGTG 
AATCGCTTCC TCTCGCAGGC GATCAACGCC AGATCCGTGG ACTGCGACAA GTCGGAGCAT 
GTGGATCGAT TGACGCTGCG CTTCCGACAG AGCGACATGG AGCGAGAGTA TCACAAGGAT 
TTCGATTTGG GGTTTACCAC CGCCATGGGA TGCTCCTTGC TGTTGCTCAT ACTCGGAGCG 
GCGCTCCAAG TGACGGCATT GCCAAGGACA CTCATACTGC TGCTGCTCTT CCTCACGGCC 
TTCATTTGGG TGAGTGCCAT ACTGATGCTC CTGCTGGCAG TGCGTCTCAA GTGGATCATT 
TGGGATATTT CGGAGAGCTT CAGCCTGCGC ATGGCCATCA CCATATTTAC AATCATTTTG 
ATTTATTCTG TGGGACAAGT GAATGTGTTC ACTTGCGTCA CGGATCATCC CTGCTCGGGA 
AATGCCACGG CCACTGCCTG GTCATCGAAC CGACAATTGG ATCCGCACTT TTTCTCGTAC 
CCAAGCGATG CCCATCGCAA GTGCTCGCTA CCACAGTACG TGTCATTGTC GGCGGCATTT 
GCCTTCCTCT CAGTATCGGT GTTTCTCAGA TTGCCCATCA TATTCAAGTC GCTGCTGGTT 
CTGGGTATGG GCACCATTTA TGGTTTGTTC ATTGAACTGT CGCATCAGAA CATCTTCGAG 
TGCTACGACC ATCGGGTGAA TGCCTCGATT CCGCTTCATT TGATCTCATT GGCGCGCATT 
GTCATCTTTA TGATTGCCAT TTTGGTGCAT GGCCGCCTGG TGGAGGGCAC CGCCCGTCTG 
GATTTTCTCT GGCAACTTCA GGCCTCCCAG GAGAAGAAGG AAATGGATGT GCTGCAGGAG 
TCGAATAAGC GCATTCTGCA CAATTTGCTG CCCGCTCATG TGGCTGCACA TTTTCTCGAT 
GCGCAATTCC GCAACAATAT GGAGCTCTAC CACCAGAGCT ATGCCAAGGT GGGCGTCATC 
TTTGCCAGCG TACCGAACTT TAATGAATTC TACACCGAAA TGGATGGCTC CGATCAGGGT 
CTGGAGTGCC TGCGTTTGCT GAACGAAATC ATTGCCGACT TTGATGAGCT GCTCAAGGAG 
GATCGCTTCA GGGGCATCGA TAAGATCAAG ACCGTCGGCA GCACCTACAT GGCCGTGGTG 
GGTCTTATAC CCGAGTACAA GATCCAGCCC AACGACCCCA ATTCGGTGCG TCGCCACATG 
ACCGCTTTGA CGGAGTATGT GAAGGCCATG CGGCTGTCGC TACAGGAGAT CAACAGTCAT 
TCGTACAATA ATTTTATGCT TCGAGTCGGC ATCAACATTG GACCCGTGGT GGCGGGCGTT 
ATTGGTGCCC GAAAGCCGCA GTACGACATC TGGGGCAATA CAGTGAATGT GGCCAGCCGC 
ATGGACTCCA CAGGCGTCCC TGGCTACTCC CAGGTCACCC AAGAGGTGGT CGACAGTCTG 
GTGGGATCGC ACTTCGAGTT TCGATGTCGA GGCACCATTA AAGTGAAGGG CAAGGGTGAC 
ATGGTCACGT ATTTCCTGTG CGACAACAGC AGCAAATCTC TGAACGGAGA GGTACGGAAT 
GCCATGTCCT TGCCGCAGAG CATGCACGGC CCCGACTACT ACATGAAGGC CTCCCAGTTC 
CCGGAGAATC GCGTCAATTC GGACACCTAC AGCAAGAAGG AGAACGGACA TCTGTACACT 
GCGGTGGGCG GCGGCGAGGA GCAGCAGCTG CTGTTGCAGC ATCAGCACAA GCAGCTGGAT 
CCCCTGCCGT TGGCAATGCC ACAGTTGGCC CTGACCGCTC CACCACAGCC ACCGGCCGTG 
CACCACCACC ACCTGCACCA GCAGCAGCAG CAGCGTCTCA ACAGCAAGCT GCAAAAGCAG 
CCCAATTTCA TGGCCAATGG GGGACTGCCA AACATACGCG AGAACGGCCA CAATGGGGAC 
CATCACTATA TGGTGGGGGC ATCAACGACG ACGACGACAA CAACACCGTC ATCTGCTGCT 
GTCCCTCTAC CCCTGGACCA GAACCAGCAC CAGATCCTAC TCCACCACCA GCTCCAACAC 
CAGCACCAGC TCCAGCTCCA GCACCAGCCC ATTCCTTCGG TAATGTTGCG CGAATTTAAT 
ATCATTGAAA ATCCGAATCC CGGTGGCAGG CATCCGCATC CCCAGCAGCA TCAGCAGCTG 
GAGCAGCTTC CGCCACAGCT GCTTGCCCAG CGGCCACAGC ATCATGGCAG CCTTAGCATG 
GACGCGGCCG GAGTGCTCGG TGGCGGTGGC GGTGGTGTCG GCAGCGGTGG CGGCGACTGC 
TTCATGATGC CGCGGCGTGA TCGCGATCGC GAACGCGAAC GAGATTGTGA TCAGCTTCAG 
CTGCGCACAT ACGCGCCGCC GCCCAGCATG GCGTTGCCCT TTAACCAGCA TGGCCATCAT 
CCAATGCATC ATCTAAACCA TTCGAATCCG CATCAGAATC AGAATCAGCA TCAGCCATCG 
TCAGCCACCA GCCTGGGGTA TGGCCACTGC CGTGAGAGCG AGCCGCTATT GCATGCCAGC 
AGCATCGCAC CGGTAGCCAA GGTGAGTGAA GATGAAGATG ATGACGATGA CGACATTGAC 
GTTGTCGAAA TGGAGAGGCT CTGGGGCAAG GAGATTCTGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002134109.1
CDS	442..5106
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVVVLCGVMAALSLAFNSAATFHNIFNSIVCLLFAVVLILLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAANALVGGAGVGLGVGLSVARGSTCEASGGGVSVGGGGVGSVQAVSEKGSRKDAVIRLQKILHATPPPHAHGMPPMGYGSVVGSGGGGGAGGGVGIDGGSVGCGIGSGCGGGGSVQVTVGTDPNSTASTISRIHRHNHKNNKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLTAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTIILIYSVGQVNVFTCVTDHPCSGNATATAWSSNRQLDPHFFSYPSDAHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDHRVNASIPLHLISLARIVIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALTEYVKAMRLSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDNSSKSLNGEVRNAMSLPQSMHGPDYYMKASQFPENRVNSDTYSKKENGHLYTAVGGGEEQQLLLQHQHKQLDPLPLAMPQLALTAPPQPPAVHHHHLHQQQQQRLNSKLQKQPNFMANGGLPNIRENGHNGDHHYMVGASTTTTTTTPSSAAVPLPLDQNQHQILLHHQLQHQHQLQLQHQPIPSVMLREFNIIENPNPGGRHPHPQQHQQLEQLPPQLLAQRPQHHGSLSMDAAGVLGGGGGGVGSGGGDCFMMPRRDRDRERERDCDQLQLRTYAPPPSMALPFNQHGHHPMHHLNHSNPHQNQNQHQPSSATSLGYGHCRESEPLLHASSIAPVAKVSEDEDDDDDDIDVVEMERLWGKEILL

Target ORF information:

RefSeq Version	XM_002134073.2
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant A (Dpse\GA29217), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002134073.2

ORF Insert Sequence:

ATGGACCATG CCGTGAAGGC AACGCGCGGC CGCCCGCTCA ATACGCTCCG CTTCGAGAAC 
GATGAGCTCG AGTGCCTCTA TCAGCGGTAC ACGCTCAAGC TCCAGCGCTT CTCGGTGCTC 
GGTGTCGTTG CACTCGTTGT GGTGCTATGC GGAGTGATGG CGGCCCTCTC GCTGGCCTTC 
AACAGTGCGG CCACCTTTCA TAACATCTTC AATTCGATCG TTTGCTTGCT GTTCGCCGTG 
GTGCTGATCC TGTTGCAATG TTCGGTGATC AAGGATCACC ATTTGCCGAC ACTGTGCTAT 
GGGATTCTAC TGTTTACGGC CAGCATTTGT GTGGTGTCGA TGCCGACGCT GGGCAGCGTC 
TTTCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGTGTCT GGCAGATCGT ATTCGTTGTA 
TTTCTGGCCT ACGCCATGAT GCCGCTGCAG ATCTGGGAGG CGGTGGCCTT CGGCATCGCC 
CTGCCCTCGG TGCACATAAG CCTCACCGTC TACAAGATCT TCACCGATGC CCTGCGATAC 
CTGGAGTACA ACCAGCTGAT TGCGAATATT GTTATCTTCA TTGGCGTGAA TGTGGCTGGG 
CTGGTGGTCA ACATTATGAT GGAGCGGGCC CAGCGAAGGA CCTTCCTCGA CACGCGCAAC 
TGTATTGCCT CTCGTCTGGA GATCCAGGAC GAGAACGAGA AGCTCGAGCG GTTGCTGCTC 
TCTGTCCTGC CCCAACATGT GGCCATGCAG ATGAAGAACG ACATCCTATC GCCGGTGGCC 
GGGCAGTTCC ATCGCATCTA CATCCAGAAG CATGAGAATG TCAGCATTCT CTTTGCGGAC 
ATTGTGGGCT TCACGGTCCT CTCGTCGCAG TGCAGTGCTC AGGAGTTGGT GCGATTGCTC 
AACGAGCTCT TTGGGCGATT CGATCAGTTG GCACATGATA ATCATTGCCT GCGCATTAAG 
ATCTTGGGCG ATTGTTATTA TTGTGTTTCG GGTTTGCCAG AGCCCCGCAA GGATCATGCA 
AAATGTGCAG TGGAAATGGG ACTCGATATG ATCGATGCCA TTGCCACTGT CGTTGAAGCC 
ACCGATGTCA TACTCAATAT GCGCGTGGGC ATCCACACTG GCCGCGTGCT GTGCGGTGTT 
TTGGGCCTCC GGAAGTGGCA GTTCGATGTG TGGTCCAACG ATGTGACCCT GGCGAATCAT 
ATGGAATCGG GCGGTGAGCC GGGTCGAGTG CATGTCACTC GCGCCACCCT CGATTCGCTG 
TCGGGCGAGT ACGAAGTGGA GGCAGGACAC GGCGACGAGC GATCCTCGTA TCTGCGCGAT 
CATGGAGTGG ATACGTTCTT TATAGTGCCA CCACCGCACC GTCGCAAGCC CCTAATGCTC 
AACACTCTGG GCGTACGCTC GGCGATCGGT AGCAGGCGGA AGCTGTCATT CCGGAATGTC 
TCCAATGTGG TCATGCAACT GTTGCACACG ATCAAGTTCT CGGAGCCAGT GCCGTTCTCG 
AATATTGCCA CCGGTTCGTT TCCTTCGGCG GCCAATGCAT TGGTGGGCGG TGCCGGTGTG 
GGTCTGGGCG TTGGTCTGAG CGTGGCACGT GGCAGCACCT GTGAGGCAAG CGGCGGCGGC 
GTCAGCGTCG GCGGCGGAGG CGTCGGCAGT GTACAGGCCG TATCGGAGAA GGGTTCACGC 
AAGGATGCGG TAATACGTCT GCAGAAAATC TTGCATGCCA CCCCGCCACC GCACGCTCAT 
GGCATGCCAC CCATGGGCTA TGGTAGCGTC GTCGGCAGTG GTGGTGGTGG TGGTGCTGGT 
GGTGGTGTCG GTATTGACGG TGGCAGTGTG GGCTGTGGTA TTGGCAGTGG CTGCGGTGGG 
GGTGGCAGTG TCCAAGTGAC CGTAGGCACA GATCCCAACT CAACAGCGAG TACGATTAGT 
CGCATTCATA GGCACAATCA CAAGAACAAT AAATCACAGT CCAAGGTGGC CGACAAATTC 
AAGAGACCCT TCCGCAAACG GCATTCGGTG GCAGCGCATC ATCAGCCCAC GAATCGTGTG 
AATCGCTTCC TCTCGCAGGC GATCAACGCC AGATCCGTGG ACTGCGACAA GTCGGAGCAT 
GTGGATCGAT TGACGCTGCG CTTCCGACAG AGCGACATGG AGCGAGAGTA TCACAAGGAT 
TTCGATTTGG GGTTTACCAC CGCCATGGGA TGCTCCTTGC TGTTGCTCAT ACTCGGAGCG 
GCGCTCCAAG TGACGGCATT GCCAAGGACA CTCATACTGC TGCTGCTCTT CCTCACGGCC 
TTCATTTGGG TGAGTGCCAT ACTGATGCTC CTGCTGGCAG TGCGTCTCAA GTGGATCATT 
TGGGATATTT CGGAGAGCTT CAGCCTGCGC ATGGCCATCA CCATATTTAC AATCATTTTG 
ATTTATTCTG TGGGACAAGT GAATGTGTTC ACTTGCGTCA CGGATCATCC CTGCTCGGGA 
AATGCCACGG CCACTGCCTG GTCATCGAAC CGACAATTGG ATCCGCACTT TTTCTCGTAC 
CCAAGCGATG CCCATCGCAA GTGCTCGCTA CCACAGTACG TGTCATTGTC GGCGGCATTT 
GCCTTCCTCT CAGTATCGGT GTTTCTCAGA TTGCCCATCA TATTCAAGTC GCTGCTGGTT 
CTGGGTATGG GCACCATTTA TGGTTTGTTC ATTGAACTGT CGCATCAGAA CATCTTCGAG 
TGCTACGACC ATCGGGTGAA TGCCTCGATT CCGCTTCATT TGATCTCATT GGCGCGCATT 
GTCATCTTTA TGATTGCCAT TTTGGTGCAT GGCCGCCTGG TGGAGGGCAC CGCCCGTCTG 
GATTTTCTCT GGCAACTTCA GGCCTCCCAG GAGAAGAAGG AAATGGATGT GCTGCAGGAG 
TCGAATAAGC GCATTCTGCA CAATTTGCTG CCCGCTCATG TGGCTGCACA TTTTCTCGAT 
GCGCAATTCC GCAACAATAT GGAGCTCTAC CACCAGAGCT ATGCCAAGGT GGGCGTCATC 
TTTGCCAGCG TACCGAACTT TAATGAATTC TACACCGAAA TGGATGGCTC CGATCAGGGT 
CTGGAGTGCC TGCGTTTGCT GAACGAAATC ATTGCCGACT TTGATGAGCT GCTCAAGGAG 
GATCGCTTCA GGGGCATCGA TAAGATCAAG ACCGTCGGCA GCACCTACAT GGCCGTGGTG 
GGTCTTATAC CCGAGTACAA GATCCAGCCC AACGACCCCA ATTCGGTGCG TCGCCACATG 
ACCGCTTTGA CGGAGTATGT GAAGGCCATG CGGCTGTCGC TACAGGAGAT CAACAGTCAT 
TCGTACAATA ATTTTATGCT TCGAGTCGGC ATCAACATTG GACCCGTGGT GGCGGGCGTT 
ATTGGTGCCC GAAAGCCGCA GTACGACATC TGGGGCAATA CAGTGAATGT GGCCAGCCGC 
ATGGACTCCA CAGGCGTCCC TGGCTACTCC CAGGTCACCC AAGAGGTGGT CGACAGTCTG 
GTGGGATCGC ACTTCGAGTT TCGATGTCGA GGCACCATTA AAGTGAAGGG CAAGGGTGAC 
ATGGTCACGT ATTTCCTGTG CGACAACAGC AGCAAATCTC TGAACGGAGA GGTACGGAAT 
GCCATGTCCT TGCCGCAGAG CATGCACGGC CCCGACTACT ACATGAAGGC CTCCCAGTTC 
CCGGAGAATC GCGTCAATTC GGACACCTAC AGCAAGAAGG AGAACGGACA TCTGTACACT 
GCGGTGGGCG GCGGCGAGGA GCAGCAGCTG CTGTTGCAGC ATCAGCACAA GCAGCTGGAT 
CCCCTGCCGT TGGCAATGCC ACAGTTGGCC CTGACCGCTC CACCACAGCC ACCGGCCGTG 
CACCACCACC ACCTGCACCA GCAGCAGCAG CAGCGTCTCA ACAGCAAGCT GCAAAAGCAG 
CCCAATTTCA TGGCCAATGG GGGACTGCCA AACATACGCG AGAACGGCCA CAATGGGGAC 
CATCACTATA TGGTGGGGGC ATCAACGACG ACGACGACAA CAACACCGTC ATCTGCTGCT 
GTCCCTCTAC CCCTGGACCA GAACCAGCAC CAGATCCTAC TCCACCACCA GCTCCAACAC 
CAGCACCAGC TCCAGCTCCA GCACCAGCCC ATTCCTTCGG TAATGTTGCG CGAATTTAAT 
ATCATTGAAA ATCCGAATCC CGGTGGCAGG CATCCGCATC CCCAGCAGCA TCAGCAGCTG 
GAGCAGCTTC CGCCACAGCT GCTTGCCCAG CGGCCACAGC ATCATGGCAG CCTTAGCATG 
GACGCGGCCG GAGTGCTCGG TGGCGGTGGC GGTGGTGTCG GCAGCGGTGG CGGCGACTGC 
TTCATGATGC CGCGGCGTGA TCGCGATCGC GAACGCGAAC GAGATTGTGA TCAGCTTCAG 
CTGCGCACAT ACGCGCCGCC GCCCAGCATG GCGTTGCCCT TTAACCAGCA TGGCCATCAT 
CCAATGCATC ATCTAAACCA TTCGAATCCG CATCAGAATC AGAATCAGCA TCAGCCATCG 
TCAGCCACCA GCCTGGGGTA TGGCCACTGC CGTGAGAGCG AGCCGCTATT GCATGCCAGC 
AGCATCGCAC CGGTAGCCAA GGTGAGTGAA GATGAAGATG ATGACGATGA CGACATTGAC 
GTTGTCGAAA TGGAGAGGCT CTGGGGCAAG GAGATTCTGC TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpse\GA29217 ( XM_015186190.1 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_015041676.1 Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA29217), mRNA.

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CloneID	ODe24819
Clone ID Related Accession (Same CDS sequence)	XM_015186190.1
Accession Version	XM_015186190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589960). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCATG CCGTGAAGGC AACGCGCGGC CGCCCGCTCA ATACGCTCCG CTTCGAGAAC 
GATGAGCTCG AGTGCCTCTA TCAGCGGTAC ACGCTCAAGC TCCAGCGCTT CTCGGTGCTC 
GGTGTCGTTG CACTCGTTGT GGTGCTATGC GGAGTGATGG CGGCCCTCTC GCTGGCCTTC 
AACAGTGCGG CCACCTTTCA TAACATCTTC AATTCGATCG TTTGCTTGCT GTTCGCCGTG 
GTGCTGATCC TGTTGCAATG TTCGGTGATC AAGGATCACC ATTTGCCGAC ACTGTGCTAT 
GGGATTCTAC TGTTTACGGC CAGCATTTGT GTGGTGTCGA TGCCGACGCT GGGCAGCGTC 
TTTCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGTGTCT GGCAGATCGT ATTCGTTGTA 
TTTCTGGCCT ACGCCATGAT GCCGCTGCAG ATCTGGGAGG CGGTGGCCTT CGGCATCGCC 
CTGCCCTCGG TGCACATAAG CCTCACCGTC TACAAGATCT TCACCGATGC CCTGCGATAC 
CTGGAGTACA ACCAGCTGAT TGCGAATATT GTTATCTTCA TTGGCGTGAA TGTGGCTGGG 
CTGGTGGTCA ACATTATGAT GGAGCGGGCC CAGCGAAGGA CCTTCCTCGA CACGCGCAAC 
TGTATTGCCT CTCGTCTGGA GATCCAGGAC GAGAACGAGA AGCTCGAGCG GTTGCTGCTC 
TCTGTCCTGC CCCAACATGT GGCCATGCAG ATGAAGAACG ACATCCTATC GCCGGTGGCC 
GGGCAGTTCC ATCGCATCTA CATCCAGAAG CATGAGAATG TCAGCATTCT CTTTGCGGAC 
ATTGTGGGCT TCACGGTCCT CTCGTCGCAG TGCAGTGCTC AGGAGTTGGT GCGATTGCTC 
AACGAGCTCT TTGGGCGATT CGATCAGTTG GCACATGATA ATCATTGCCT GCGCATTAAG 
ATCTTGGGCG ATTGTTATTA TTGTGTTTCG GGTTTGCCAG AGCCCCGCAA GGATCATGCA 
AAATGTGCAG TGGAAATGGG ACTCGATATG ATCGATGCCA TTGCCACTGT CGTTGAAGCC 
ACCGATGTCA TACTCAATAT GCGCGTGGGC ATCCACACTG GCCGCGTGCT GTGCGGTGTT 
TTGGGCCTCC GGAAGTGGCA GTTCGATGTG TGGTCCAACG ATGTGACCCT GGCGAATCAT 
ATGGAATCGG GCGGTGAGCC GGGTCGAGTG CATGTCACTC GCGCCACCCT CGATTCGCTG 
TCGGGCGAGT ACGAAGTGGA GGCAGGACAC GGCGACGAGC GATCCTCGTA TCTGCGCGAT 
CATGGAGTGG ATACGTTCTT TATAGTGCCA CCACCGCACC GTCGCAAGCC CCTAATGCTC 
AACACTCTGG GCGTACGCTC GGCGATCGGT AGCAGGCGGA AGCTGTCATT CCGGAATGTC 
TCCAATGTGG TCATGCAACT GTTGCACACG ATCAAGTTCT CGGAGCCAGT GCCGTTCTCG 
AATATTGCCA CCGGTTCGTT TCCTTCGGCG GCCAATGCAT TGGTGGGCGG TGCCGGTGTG 
GGTCTGGGCG TTGGTCTGAG CGTGGCACGT GGCAGCACCT GTGAGGCAAG CGGCGGCGGC 
GTCAGCGTCG GCGGCGGAGG CGTCGGCAGT GTACAGGCCG TATCGGAGAA GGGTTCACGC 
AAGGTAATAC GTCTGCAGAA AATCTTGCAT GCCACCCCGC CACCGCACGC TCATGGCATG 
CCACCCATGG GCTATGGTAG CGTCGTCGGC AGTGGTGGTG GTGGTGGTGC TGGTGGTGGT 
GTCGGTATTG ACGGTGGCAG TGTGGGCTGT GGTATTGGCA GTGGCTGCGG TGGGGGTGGC 
AGTGTCCAAG TGACCGTAGG CACAGATCCC AACTCAACAG CGAGTACGAT TAGTCGCATT 
CATAGGCACA ATCACAAGAA CAATAAATCA CAGTCCAAGG TGGCCGACAA ATTCAAGAGA 
CCCTTCCGCA AACGGCATTC GGTGGCAGCG CATCATCAGC CCACGAATCG TGTGAATCGC 
TTCCTCTCGC AGGCGATCAA CGCCAGATCC GTGGACTGCG ACAAGTCGGA GCATGTGGAT 
CGATTGACGC TGCGCTTCCG ACAGAGCGAC ATGGAGCGAG AGTATCACAA GGATTTCGAT 
TTGGGGTTTA CCACCGCCAT GGGATGCTCC TTGCTGTTGC TCATACTCGG AGCGGCGCTC 
CAAGTGACGG CATTGCCAAG GACACTCATA CTGCTGCTGC TCTTCCTCAC GGCCTTCATT 
TGGGTGAGTG CCATACTGAT GCTCCTGCTG GCAGTGCGTC TCAAGTGGAT CATTTGGGAT 
ATTTCGGAGA GCTTCAGCCT GCGCATGGCC ATCACCATAT TTACAATCAT TTTGATTTAT 
TCTGTGGGAC AAGTGAATGT GTTCACTTGC GTCACGGATC ATCCCTGCTC GGGAAATGCC 
ACGGCCACTG CCTGGTCATC GAACCGACAA TTGGATCCGC ACTTTTTCTC GTACCCAAGC 
GATGCCCATC GCAAGTGCTC GCTACCACAG TACGTGTCAT TGTCGGCGGC ATTTGCCTTC 
CTCTCAGTAT CGGTGTTTCT CAGATTGCCC ATCATATTCA AGTCGCTGCT GGTTCTGGGT 
ATGGGCACCA TTTATGGTTT GTTCATTGAA CTGTCGCATC AGAACATCTT CGAGTGCTAC 
GACCATCGGG TGAATGCCTC GATTCCGCTT CATTTGATCT CATTGGCGCG CATTGTCATC 
TTTATGATTG CCATTTTGGT GCATGGCCGC CTGGTGGAGG GCACCGCCCG TCTGGATTTT 
CTCTGGCAAC TTCAGGCCTC CCAGGAGAAG AAGGAAATGG ATGTGCTGCA GGAGTCGAAT 
AAGCGCATTC TGCACAATTT GCTGCCCGCT CATGTGGCTG CACATTTTCT CGATGCGCAA 
TTCCGCAACA ATATGGAGCT CTACCACCAG AGCTATGCCA AGGTGGGCGT CATCTTTGCC 
AGCGTACCGA ACTTTAATGA ATTCTACACC GAAATGGATG GCTCCGATCA GGGTCTGGAG 
TGCCTGCGTT TGCTGAACGA AATCATTGCC GACTTTGATG AGCTGCTCAA GGAGGATCGC 
TTCAGGGGCA TCGATAAGAT CAAGACCGTC GGCAGCACCT ACATGGCCGT GGTGGGTCTT 
ATACCCGAGT ACAAGATCCA GCCCAACGAC CCCAATTCGG TGCGTCGCCA CATGACCGCT 
TTGACGGAGT ATGTGAAGGC CATGCGGCTG TCGCTACAGG AGATCAACAG TCATTCGTAC 
AATAATTTTA TGCTTCGAGT CGGCATCAAC ATTGGACCCG TGGTGGCGGG CGTTATTGGT 
GCCCGAAAGC CGCAGTACGA CATCTGGGGC AATACAGTGA ATGTGGCCAG CCGCATGGAC 
TCCACAGGCG TCCCTGGCTA CTCCCAGGTC ACCCAAGAGG TGGTCGACAG TCTGGTGGGA 
TCGCACTTCG AGTTTCGATG TCGAGGCACC ATTAAAGTGA AGGGCAAGGG TGACATGGTC 
ACGTATTTCC TGTGCGACAA CAGCAGCAAA TCTCTGAACG GAGAGGTACG GAATGCCATG 
TCCTTGCCGC AGAGCATGCA CGGCCCCGAC TACTACATGA AGGCCTCCCA GTTCCCGGAG 
AATCGCGTCA ATTCGGACAC CTACAGCAAG AAGGAGAACG GACATCTGTA CACTGCGGTG 
GGCGGCGGCG AGGAGCAGCA GCTGCTGTTG CAGCATCAGC ACAAGCAGCT GGATCCCCTG 
CCGTTGGCAA TGCCACAGTT GGCCCTGACC GCTCCACCAC AGCCACCGGC CGTGCACCAC 
CACCACCTGC ACCAGCAGCA GCAGCAGCGT CTCAACAGCA AGCTGCAAAA GCAGCCCAAT 
TTCATGGCCA ATGGGGGACT GCCAAACATA CGCGAGAACG GCCACAATGG GGACCATCAC 
TATATGGTGG GGGCATCAAC GACGACGACG ACAACAACAC CGTCATCTGC TGCTGTCCCT 
CTACCCCTGG ACCAGAACCA GCACCAGATC CTACTCCACC ACCAGCTCCA ACACCAGCAC 
CAGCTCCAGC TCCAGCACCA GCCCATTCCT TCGGTAATGT TGCGCGAATT TAATATCATT 
GAAAATCCGA ATCCCGGTGG CAGGCATCCG CATCCCCAGC AGCATCAGCA GCTGGAGCAG 
CTTCCGCCAC AGCTGCTTGC CCAGCGGCCA CAGCATCATG GCAGCCTTAG CATGGACGCG 
GCCGGAGTGC TCGGTGGCGG TGGCGGTGGT GTCGGCAGCG GTGGCGGCGA CTGCTTCATG 
ATGCCGCGGC GTGATCGCGA TCGCGAACGC GAACGAGATT GTGATCAGCT TCAGCTGCGC 
ACATACGCGC CGCCGCCCAG CATGGCGTTG CCCTTTAACC AGCATGGCCA TCATCCAATG 
CATCATCTAA ACCATTCGAA TCCGCATCAG AATCAGAATC AGCATCAGCC ATCGTCAGCC 
ACCAGCCTGG GGTATGGCCA CTGCCGTGAG AGCGAGCCGC TATTGCATGC CAGCAGCATC 
GCACCGGTAG CCAAGATCAT GCCCATGCAG CATGCGCCAA AGTACGAGCC ACCACGTTAC 
ACTTCTCCCA ACACCGTGTT GTCACAGCAT TCGCAACAGC AGCAGCAGCA GTCGCTTCCT 
GCCCCACAGC AGCTGCTGTA CGGACACCAA GTGATGCAAC AGCAGCAGCA ATATGCCATG 
TACTCGCAAC AGGTGCCCGT GGTCATGCCG GTGCCGGTGC CGGTGCCACT GCCACCGAAG 
CCCACGCTGC GGACGTACAT GAAGCCGCTG CCCAAGCTTC CCGCAGAGTT GGACGAGAGC 
CGTGACATGT CCTCAACGGA TGACCTAAGC TCTCGGCCGC ACTCCCCATC GATGAGCAGC 
TCCGATGAGA GCTACTCGAA GACAACGGAG GGCGAGGGCG ATGAGGACTC GCCCAGGATC 
AATGGCAGCC ACCACCATCA CCAGCTCAAT CGGAATGGCC ATCCCGTGGG CTTCCATCTG 
ACTGGCATAG GTGGAGGAGG AGGAGGAGGA GCAGCAGCAG GAGGAGGAGG ACTCGTGAAT 
CCCCTGCAAT GGCTGTACCC GTGCGACATT CAAGTGGATC CCACCTCGCC AGTCGTGGAC 
ATGACCCAGC TGCAGGACTT TGAGCTTAGC TCTACCACCG AGAGCCAGGG CCAGCACACG 
ACCAGCAACG CGACGAGCAA CAACACCCAG AACAAGGGCG ACAGTTGCAA CAGTTTCGAC 
TACCAAAAGG CAGGAGGATC TGCCCAGGGG GGGCAGCAGC AGCCTGTTGC TGCCACCACC 
AAATCCCCCT TCGAAAGGGA GCTGCAGCGA TTGCTCAATG AATCCTCGAG GGCGCGCTGC 
CTGGCAGCAG CCAACGCCAG TGCCACAGAG AATGGCAAGC AGATGAGCTA TTCCGTCAGC 
AACAGCAAGC TGGCGGGAGC GCAGGGAGGA GCGGGTACGG GAACGGGAAC AGGAGGAGCC 
ACAGCAGGCA ATGGTGGCAG CGGCGGCAGT GGCAGCAGCA ATGGAAACCT CAGCGGTGGC 
AGCAACTCGA ATGCCAGCGG CAACAACAAT CATCAGAACG GCCGCGAGCG GGAGCGGGAG 
CGAGAGCAGC AGGAGCACGA CCTGATGGGC CGTCTACCCG CAAGCAGCAG TTTCATGATT 
GCCAAGCACC CTGTGGGCCT GGAGGCCATC AAGGAGATAA CGCGCAACAA GAATCCCTCG 
GAATCGAGTC AAATGCAAAC ATCGGACACG GAGTCCTGCG AGATTCTGCA CGAGAATCGC 
AATCAACTGC ATGCCCTGTC GCATTCCCAT CAGATGATGG AGCAGCAGCA GCCGCAGTTG 
CAACAGTCGC AGCGGGCACA TCGCCAACGG CCACGCTCCA AGGAGCTGCA ATACAGCCAC 
GAGAGTCTGG ATGGCCTGCA GGAGCAGCAG CAACAGCAGC AGCAGCGGCA CCCGCGCTAC 
CATCACCATC ACTCCCACCA CCATCATCAT CATCATCACC AGCAACAGCA GCAACAGCAG 
CAGCGATACA ACCATGTCCA GGAGCAGGAG GAGCGTGACG ACACAGAGGA TAACCTGGCC 
GACGAGGAGT TCGAAGACGA TGAAGTGGGT CGGGATGTGC GACAAAAGCG CCTGCTGCAG 
CAGGCCCCAG GAGAAAAGCA TAAGCGAACG ACTGGGGATA GCGATGATGA TGATGATGAG 
GAGGAGGATC ACGAGCATGA GCAGAAGGAG CAGAACAATG TGACACCGCT GACAAACGGC 
GTTTGCGGTG GCGGTGGCCT GCGCAGCGGC GGCCTGGAGT CGAATGTGAT AAACGATGAG 
CTAAAATATG GGGCAGCACA TCTCAACCAC CAGTCGATGG ACTCCAATCC ATTGGAGAGC 
CAATCGGAAT GGTCGGATGA TGATTGCCGT GAAGAGGCCA CAGGTGGTGC CGAGTCCACT 
GGGTATATTA CGGATGAACC GGGCCTGGAA AATATCTCGC TGCTGAATGA GGCCGGTCTC 
ACCGATGCCG AGGGCGCTCT CTCGGATGTC AATTCGCTGT ACAATGCCCC CGATGTGGAT 
GACACCTCAG TGTCGAGTCG CGCCAGCTCT CGTCTGTTAA GCCTGGACTC GCTGAGCGGT 
CTGTACGATT GTGATCTGGA CTCCAAGCAT GAGCTGGCCA TTGTCAATGC CTCGCACAAG 
ATAACCAGCA AATTTGGACC GCCGCCATCG CCGGCACAAC ATCAGCAGCA GCTGCAGCAC 
CAGCCGCAGC ACCAGCCGCA GCACCAGCCG CAGTCGCAGT CTCAGTCTTT GGTTACGGCC 
TCGTCTTTGG TTTCGGTTCA GGTTCCAACT CAGGCACAGA CTCAATCGCA ATCAGCGGAA 
GAGCTGAGGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015041676.1
CDS	442..7356
Translation	MDHAVKATRGRPLNTLRFENDELECLYQRYTLKLQRFSVLGVVALVVVLCGVMAALSLAFNSAATFHNIFNSIVCLLFAVVLILLQCSVIKDHHLPTLCYGILLFTASICVVSMPTLGSVFPVDTKEVMAEGVWQIVFVVFLAYAMMPLQIWEAVAFGIALPSVHISLTVYKIFTDALRYLEYNQLIANIVIFIGVNVAGLVVNIMMERAQRRTFLDTRNCIASRLEIQDENEKLERLLLSVLPQHVAMQMKNDILSPVAGQFHRIYIQKHENVSILFADIVGFTVLSSQCSAQELVRLLNELFGRFDQLAHDNHCLRIKILGDCYYCVSGLPEPRKDHAKCAVEMGLDMIDAIATVVEATDVILNMRVGIHTGRVLCGVLGLRKWQFDVWSNDVTLANHMESGGEPGRVHVTRATLDSLSGEYEVEAGHGDERSSYLRDHGVDTFFIVPPPHRRKPLMLNTLGVRSAIGSRRKLSFRNVSNVVMQLLHTIKFSEPVPFSNIATGSFPSAANALVGGAGVGLGVGLSVARGSTCEASGGGVSVGGGGVGSVQAVSEKGSRKVIRLQKILHATPPPHAHGMPPMGYGSVVGSGGGGGAGGGVGIDGGSVGCGIGSGCGGGGSVQVTVGTDPNSTASTISRIHRHNHKNNKSQSKVADKFKRPFRKRHSVAAHHQPTNRVNRFLSQAINARSVDCDKSEHVDRLTLRFRQSDMEREYHKDFDLGFTTAMGCSLLLLILGAALQVTALPRTLILLLLFLTAFIWVSAILMLLLAVRLKWIIWDISESFSLRMAITIFTIILIYSVGQVNVFTCVTDHPCSGNATATAWSSNRQLDPHFFSYPSDAHRKCSLPQYVSLSAAFAFLSVSVFLRLPIIFKSLLVLGMGTIYGLFIELSHQNIFECYDHRVNASIPLHLISLARIVIFMIAILVHGRLVEGTARLDFLWQLQASQEKKEMDVLQESNKRILHNLLPAHVAAHFLDAQFRNNMELYHQSYAKVGVIFASVPNFNEFYTEMDGSDQGLECLRLLNEIIADFDELLKEDRFRGIDKIKTVGSTYMAVVGLIPEYKIQPNDPNSVRRHMTALTEYVKAMRLSLQEINSHSYNNFMLRVGINIGPVVAGVIGARKPQYDIWGNTVNVASRMDSTGVPGYSQVTQEVVDSLVGSHFEFRCRGTIKVKGKGDMVTYFLCDNSSKSLNGEVRNAMSLPQSMHGPDYYMKASQFPENRVNSDTYSKKENGHLYTAVGGGEEQQLLLQHQHKQLDPLPLAMPQLALTAPPQPPAVHHHHLHQQQQQRLNSKLQKQPNFMANGGLPNIRENGHNGDHHYMVGASTTTTTTTPSSAAVPLPLDQNQHQILLHHQLQHQHQLQLQHQPIPSVMLREFNIIENPNPGGRHPHPQQHQQLEQLPPQLLAQRPQHHGSLSMDAAGVLGGGGGGVGSGGGDCFMMPRRDRDRERERDCDQLQLRTYAPPPSMALPFNQHGHHPMHHLNHSNPHQNQNQHQPSSATSLGYGHCRESEPLLHASSIAPVAKIMPMQHAPKYEPPRYTSPNTVLSQHSQQQQQQSLPAPQQLLYGHQVMQQQQQYAMYSQQVPVVMPVPVPVPLPPKPTLRTYMKPLPKLPAELDESRDMSSTDDLSSRPHSPSMSSSDESYSKTTEGEGDEDSPRINGSHHHHQLNRNGHPVGFHLTGIGGGGGGGAAAGGGGLVNPLQWLYPCDIQVDPTSPVVDMTQLQDFELSSTTESQGQHTTSNATSNNTQNKGDSCNSFDYQKAGGSAQGGQQQPVAATTKSPFERELQRLLNESSRARCLAAANASATENGKQMSYSVSNSKLAGAQGGAGTGTGTGGATAGNGGSGGSGSSNGNLSGGSNSNASGNNNHQNGREREREREQQEHDLMGRLPASSSFMIAKHPVGLEAIKEITRNKNPSESSQMQTSDTESCEILHENRNQLHALSHSHQMMEQQQPQLQQSQRAHRQRPRSKELQYSHESLDGLQEQQQQQQQRHPRYHHHHSHHHHHHHHQQQQQQQQRYNHVQEQEERDDTEDNLADEEFEDDEVGRDVRQKRLLQQAPGEKHKRTTGDSDDDDDEEEDHEHEQKEQNNVTPLTNGVCGGGGLRSGGLESNVINDELKYGAAHLNHQSMDSNPLESQSEWSDDDCREEATGGAESTGYITDEPGLENISLLNEAGLTDAEGALSDVNSLYNAPDVDDTSVSSRASSRLLSLDSLSGLYDCDLDSKHELAIVNASHKITSKFGPPPSPAQHQQQLQHQPQHQPQHQPQSQSQSLVTASSLVSVQVPTQAQTQSQSAEELRE

Target ORF information:

RefSeq Version	XM_015186190.1
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein, transcript variant B (Dpse\GA29217), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015186190.1

ORF Insert Sequence:

ATGGACCATG CCGTGAAGGC AACGCGCGGC CGCCCGCTCA ATACGCTCCG CTTCGAGAAC 
GATGAGCTCG AGTGCCTCTA TCAGCGGTAC ACGCTCAAGC TCCAGCGCTT CTCGGTGCTC 
GGTGTCGTTG CACTCGTTGT GGTGCTATGC GGAGTGATGG CGGCCCTCTC GCTGGCCTTC 
AACAGTGCGG CCACCTTTCA TAACATCTTC AATTCGATCG TTTGCTTGCT GTTCGCCGTG 
GTGCTGATCC TGTTGCAATG TTCGGTGATC AAGGATCACC ATTTGCCGAC ACTGTGCTAT 
GGGATTCTAC TGTTTACGGC CAGCATTTGT GTGGTGTCGA TGCCGACGCT GGGCAGCGTC 
TTTCCGGTGG ACACCAAGGA GGTGATGGCC GAGGGTGTCT GGCAGATCGT ATTCGTTGTA 
TTTCTGGCCT ACGCCATGAT GCCGCTGCAG ATCTGGGAGG CGGTGGCCTT CGGCATCGCC 
CTGCCCTCGG TGCACATAAG CCTCACCGTC TACAAGATCT TCACCGATGC CCTGCGATAC 
CTGGAGTACA ACCAGCTGAT TGCGAATATT GTTATCTTCA TTGGCGTGAA TGTGGCTGGG 
CTGGTGGTCA ACATTATGAT GGAGCGGGCC CAGCGAAGGA CCTTCCTCGA CACGCGCAAC 
TGTATTGCCT CTCGTCTGGA GATCCAGGAC GAGAACGAGA AGCTCGAGCG GTTGCTGCTC 
TCTGTCCTGC CCCAACATGT GGCCATGCAG ATGAAGAACG ACATCCTATC GCCGGTGGCC 
GGGCAGTTCC ATCGCATCTA CATCCAGAAG CATGAGAATG TCAGCATTCT CTTTGCGGAC 
ATTGTGGGCT TCACGGTCCT CTCGTCGCAG TGCAGTGCTC AGGAGTTGGT GCGATTGCTC 
AACGAGCTCT TTGGGCGATT CGATCAGTTG GCACATGATA ATCATTGCCT GCGCATTAAG 
ATCTTGGGCG ATTGTTATTA TTGTGTTTCG GGTTTGCCAG AGCCCCGCAA GGATCATGCA 
AAATGTGCAG TGGAAATGGG ACTCGATATG ATCGATGCCA TTGCCACTGT CGTTGAAGCC 
ACCGATGTCA TACTCAATAT GCGCGTGGGC ATCCACACTG GCCGCGTGCT GTGCGGTGTT 
TTGGGCCTCC GGAAGTGGCA GTTCGATGTG TGGTCCAACG ATGTGACCCT GGCGAATCAT 
ATGGAATCGG GCGGTGAGCC GGGTCGAGTG CATGTCACTC GCGCCACCCT CGATTCGCTG 
TCGGGCGAGT ACGAAGTGGA GGCAGGACAC GGCGACGAGC GATCCTCGTA TCTGCGCGAT 
CATGGAGTGG ATACGTTCTT TATAGTGCCA CCACCGCACC GTCGCAAGCC CCTAATGCTC 
AACACTCTGG GCGTACGCTC GGCGATCGGT AGCAGGCGGA AGCTGTCATT CCGGAATGTC 
TCCAATGTGG TCATGCAACT GTTGCACACG ATCAAGTTCT CGGAGCCAGT GCCGTTCTCG 
AATATTGCCA CCGGTTCGTT TCCTTCGGCG GCCAATGCAT TGGTGGGCGG TGCCGGTGTG 
GGTCTGGGCG TTGGTCTGAG CGTGGCACGT GGCAGCACCT GTGAGGCAAG CGGCGGCGGC 
GTCAGCGTCG GCGGCGGAGG CGTCGGCAGT GTACAGGCCG TATCGGAGAA GGGTTCACGC 
AAGGTAATAC GTCTGCAGAA AATCTTGCAT GCCACCCCGC CACCGCACGC TCATGGCATG 
CCACCCATGG GCTATGGTAG CGTCGTCGGC AGTGGTGGTG GTGGTGGTGC TGGTGGTGGT 
GTCGGTATTG ACGGTGGCAG TGTGGGCTGT GGTATTGGCA GTGGCTGCGG TGGGGGTGGC 
AGTGTCCAAG TGACCGTAGG CACAGATCCC AACTCAACAG CGAGTACGAT TAGTCGCATT 
CATAGGCACA ATCACAAGAA CAATAAATCA CAGTCCAAGG TGGCCGACAA ATTCAAGAGA 
CCCTTCCGCA AACGGCATTC GGTGGCAGCG CATCATCAGC CCACGAATCG TGTGAATCGC 
TTCCTCTCGC AGGCGATCAA CGCCAGATCC GTGGACTGCG ACAAGTCGGA GCATGTGGAT 
CGATTGACGC TGCGCTTCCG ACAGAGCGAC ATGGAGCGAG AGTATCACAA GGATTTCGAT 
TTGGGGTTTA CCACCGCCAT GGGATGCTCC TTGCTGTTGC TCATACTCGG AGCGGCGCTC 
CAAGTGACGG CATTGCCAAG GACACTCATA CTGCTGCTGC TCTTCCTCAC GGCCTTCATT 
TGGGTGAGTG CCATACTGAT GCTCCTGCTG GCAGTGCGTC TCAAGTGGAT CATTTGGGAT 
ATTTCGGAGA GCTTCAGCCT GCGCATGGCC ATCACCATAT TTACAATCAT TTTGATTTAT 
TCTGTGGGAC AAGTGAATGT GTTCACTTGC GTCACGGATC ATCCCTGCTC GGGAAATGCC 
ACGGCCACTG CCTGGTCATC GAACCGACAA TTGGATCCGC ACTTTTTCTC GTACCCAAGC 
GATGCCCATC GCAAGTGCTC GCTACCACAG TACGTGTCAT TGTCGGCGGC ATTTGCCTTC 
CTCTCAGTAT CGGTGTTTCT CAGATTGCCC ATCATATTCA AGTCGCTGCT GGTTCTGGGT 
ATGGGCACCA TTTATGGTTT GTTCATTGAA CTGTCGCATC AGAACATCTT CGAGTGCTAC 
GACCATCGGG TGAATGCCTC GATTCCGCTT CATTTGATCT CATTGGCGCG CATTGTCATC 
TTTATGATTG CCATTTTGGT GCATGGCCGC CTGGTGGAGG GCACCGCCCG TCTGGATTTT 
CTCTGGCAAC TTCAGGCCTC CCAGGAGAAG AAGGAAATGG ATGTGCTGCA GGAGTCGAAT 
AAGCGCATTC TGCACAATTT GCTGCCCGCT CATGTGGCTG CACATTTTCT CGATGCGCAA 
TTCCGCAACA ATATGGAGCT CTACCACCAG AGCTATGCCA AGGTGGGCGT CATCTTTGCC 
AGCGTACCGA ACTTTAATGA ATTCTACACC GAAATGGATG GCTCCGATCA GGGTCTGGAG 
TGCCTGCGTT TGCTGAACGA AATCATTGCC GACTTTGATG AGCTGCTCAA GGAGGATCGC 
TTCAGGGGCA TCGATAAGAT CAAGACCGTC GGCAGCACCT ACATGGCCGT GGTGGGTCTT 
ATACCCGAGT ACAAGATCCA GCCCAACGAC CCCAATTCGG TGCGTCGCCA CATGACCGCT 
TTGACGGAGT ATGTGAAGGC CATGCGGCTG TCGCTACAGG AGATCAACAG TCATTCGTAC 
AATAATTTTA TGCTTCGAGT CGGCATCAAC ATTGGACCCG TGGTGGCGGG CGTTATTGGT 
GCCCGAAAGC CGCAGTACGA CATCTGGGGC AATACAGTGA ATGTGGCCAG CCGCATGGAC 
TCCACAGGCG TCCCTGGCTA CTCCCAGGTC ACCCAAGAGG TGGTCGACAG TCTGGTGGGA 
TCGCACTTCG AGTTTCGATG TCGAGGCACC ATTAAAGTGA AGGGCAAGGG TGACATGGTC 
ACGTATTTCC TGTGCGACAA CAGCAGCAAA TCTCTGAACG GAGAGGTACG GAATGCCATG 
TCCTTGCCGC AGAGCATGCA CGGCCCCGAC TACTACATGA AGGCCTCCCA GTTCCCGGAG 
AATCGCGTCA ATTCGGACAC CTACAGCAAG AAGGAGAACG GACATCTGTA CACTGCGGTG 
GGCGGCGGCG AGGAGCAGCA GCTGCTGTTG CAGCATCAGC ACAAGCAGCT GGATCCCCTG 
CCGTTGGCAA TGCCACAGTT GGCCCTGACC GCTCCACCAC AGCCACCGGC CGTGCACCAC 
CACCACCTGC ACCAGCAGCA GCAGCAGCGT CTCAACAGCA AGCTGCAAAA GCAGCCCAAT 
TTCATGGCCA ATGGGGGACT GCCAAACATA CGCGAGAACG GCCACAATGG GGACCATCAC 
TATATGGTGG GGGCATCAAC GACGACGACG ACAACAACAC CGTCATCTGC TGCTGTCCCT 
CTACCCCTGG ACCAGAACCA GCACCAGATC CTACTCCACC ACCAGCTCCA ACACCAGCAC 
CAGCTCCAGC TCCAGCACCA GCCCATTCCT TCGGTAATGT TGCGCGAATT TAATATCATT 
GAAAATCCGA ATCCCGGTGG CAGGCATCCG CATCCCCAGC AGCATCAGCA GCTGGAGCAG 
CTTCCGCCAC AGCTGCTTGC CCAGCGGCCA CAGCATCATG GCAGCCTTAG CATGGACGCG 
GCCGGAGTGC TCGGTGGCGG TGGCGGTGGT GTCGGCAGCG GTGGCGGCGA CTGCTTCATG 
ATGCCGCGGC GTGATCGCGA TCGCGAACGC GAACGAGATT GTGATCAGCT TCAGCTGCGC 
ACATACGCGC CGCCGCCCAG CATGGCGTTG CCCTTTAACC AGCATGGCCA TCATCCAATG 
CATCATCTAA ACCATTCGAA TCCGCATCAG AATCAGAATC AGCATCAGCC ATCGTCAGCC 
ACCAGCCTGG GGTATGGCCA CTGCCGTGAG AGCGAGCCGC TATTGCATGC CAGCAGCATC 
GCACCGGTAG CCAAGATCAT GCCCATGCAG CATGCGCCAA AGTACGAGCC ACCACGTTAC 
ACTTCTCCCA ACACCGTGTT GTCACAGCAT TCGCAACAGC AGCAGCAGCA GTCGCTTCCT 
GCCCCACAGC AGCTGCTGTA CGGACACCAA GTGATGCAAC AGCAGCAGCA ATATGCCATG 
TACTCGCAAC AGGTGCCCGT GGTCATGCCG GTGCCGGTGC CGGTGCCACT GCCACCGAAG 
CCCACGCTGC GGACGTACAT GAAGCCGCTG CCCAAGCTTC CCGCAGAGTT GGACGAGAGC 
CGTGACATGT CCTCAACGGA TGACCTAAGC TCTCGGCCGC ACTCCCCATC GATGAGCAGC 
TCCGATGAGA GCTACTCGAA GACAACGGAG GGCGAGGGCG ATGAGGACTC GCCCAGGATC 
AATGGCAGCC ACCACCATCA CCAGCTCAAT CGGAATGGCC ATCCCGTGGG CTTCCATCTG 
ACTGGCATAG GTGGAGGAGG AGGAGGAGGA GCAGCAGCAG GAGGAGGAGG ACTCGTGAAT 
CCCCTGCAAT GGCTGTACCC GTGCGACATT CAAGTGGATC CCACCTCGCC AGTCGTGGAC 
ATGACCCAGC TGCAGGACTT TGAGCTTAGC TCTACCACCG AGAGCCAGGG CCAGCACACG 
ACCAGCAACG CGACGAGCAA CAACACCCAG AACAAGGGCG ACAGTTGCAA CAGTTTCGAC 
TACCAAAAGG CAGGAGGATC TGCCCAGGGG GGGCAGCAGC AGCCTGTTGC TGCCACCACC 
AAATCCCCCT TCGAAAGGGA GCTGCAGCGA TTGCTCAATG AATCCTCGAG GGCGCGCTGC 
CTGGCAGCAG CCAACGCCAG TGCCACAGAG AATGGCAAGC AGATGAGCTA TTCCGTCAGC 
AACAGCAAGC TGGCGGGAGC GCAGGGAGGA GCGGGTACGG GAACGGGAAC AGGAGGAGCC 
ACAGCAGGCA ATGGTGGCAG CGGCGGCAGT GGCAGCAGCA ATGGAAACCT CAGCGGTGGC 
AGCAACTCGA ATGCCAGCGG CAACAACAAT CATCAGAACG GCCGCGAGCG GGAGCGGGAG 
CGAGAGCAGC AGGAGCACGA CCTGATGGGC CGTCTACCCG CAAGCAGCAG TTTCATGATT 
GCCAAGCACC CTGTGGGCCT GGAGGCCATC AAGGAGATAA CGCGCAACAA GAATCCCTCG 
GAATCGAGTC AAATGCAAAC ATCGGACACG GAGTCCTGCG AGATTCTGCA CGAGAATCGC 
AATCAACTGC ATGCCCTGTC GCATTCCCAT CAGATGATGG AGCAGCAGCA GCCGCAGTTG 
CAACAGTCGC AGCGGGCACA TCGCCAACGG CCACGCTCCA AGGAGCTGCA ATACAGCCAC 
GAGAGTCTGG ATGGCCTGCA GGAGCAGCAG CAACAGCAGC AGCAGCGGCA CCCGCGCTAC 
CATCACCATC ACTCCCACCA CCATCATCAT CATCATCACC AGCAACAGCA GCAACAGCAG 
CAGCGATACA ACCATGTCCA GGAGCAGGAG GAGCGTGACG ACACAGAGGA TAACCTGGCC 
GACGAGGAGT TCGAAGACGA TGAAGTGGGT CGGGATGTGC GACAAAAGCG CCTGCTGCAG 
CAGGCCCCAG GAGAAAAGCA TAAGCGAACG ACTGGGGATA GCGATGATGA TGATGATGAG 
GAGGAGGATC ACGAGCATGA GCAGAAGGAG CAGAACAATG TGACACCGCT GACAAACGGC 
GTTTGCGGTG GCGGTGGCCT GCGCAGCGGC GGCCTGGAGT CGAATGTGAT AAACGATGAG 
CTAAAATATG GGGCAGCACA TCTCAACCAC CAGTCGATGG ACTCCAATCC ATTGGAGAGC 
CAATCGGAAT GGTCGGATGA TGATTGCCGT GAAGAGGCCA CAGGTGGTGC CGAGTCCACT 
GGGTATATTA CGGATGAACC GGGCCTGGAA AATATCTCGC TGCTGAATGA GGCCGGTCTC 
ACCGATGCCG AGGGCGCTCT CTCGGATGTC AATTCGCTGT ACAATGCCCC CGATGTGGAT 
GACACCTCAG TGTCGAGTCG CGCCAGCTCT CGTCTGTTAA GCCTGGACTC GCTGAGCGGT 
CTGTACGATT GTGATCTGGA CTCCAAGCAT GAGCTGGCCA TTGTCAATGC CTCGCACAAG 
ATAACCAGCA AATTTGGACC GCCGCCATCG CCGGCACAAC ATCAGCAGCA GCTGCAGCAC 
CAGCCGCAGC ACCAGCCGCA GCACCAGCCG CAGTCGCAGT CTCAGTCTTT GGTTACGGCC 
TCGTCTTTGG TTTCGGTTCA GGTTCCAACT CAGGCACAGA CTCAATCGCA ATCAGCGGAA 
GAGCTGAGGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
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Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA29217 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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