Dpse\GA19103 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpse\GA19103
Entrez Gene ID	4812697
Full Name	GA19103 gene product from transcript GA19103-RB
Synonyms	GA19103,dpse_GLEANR_8706,mfr
Gene Type	protein-coding
Organism	Drosophila pseudoobscura pseudoobscura

mRNA and Protein(s)

mRNA	Protein	Name
XM_001353043.3	XP_001353079.3	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
English AC, Richards S, Han Y, Wang M, Vee V, Qu J, Qin X, Muzny DM, Reid JG, Worley KC, Gibbs RA
PloS one7(11)e47768(2012)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Richards S, Liu Y, Bettencourt BR, Hradecky P, Letovsky S, Nielsen R, Thornton K, Hubisz MJ, Chen R, Meisel RP, Couronne O, Hua S, Smith MA, Zhang P, Liu J, Bussemaker HJ, van Batenburg MF, Howells SL, Scherer SE, Sodergren E, Matthews BB, Crosby MA, Schroeder AJ, Ortiz-Barrientos D, Rives CM, Metzker ML, Muzny DM, Scott G, Steffen D, Wheeler DA, Worley KC, Havlak P, Durbin KJ, Egan A, Gill R, Hume J, Morgan MB, Miner G, Hamilton C, Huang Y, Waldron L, Verduzco D, Clerc-Blankenburg KP, Dubchak I, Noor MA, Anderson W, White KP, Clark AG, Schaeffer SW, Gelbart W, Weinstock GM, Gibbs RA
Genome research15(1)1-18(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpse\GA19103 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpse\GA19103 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe26990	XM_001353043.3 Latest version!	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA19103), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpse\GA19103 ( XM_001353043.3 ) cDNA ORF clone, Drosophila pseudoobscura pseudoobscura -> XP_001353079.3 Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA19103), mRNA.

Price & Availability↑

CloneID	ODe26990
Clone ID Related Accession (Same CDS sequence)	XM_001353043.3
Accession Version	XM_001353043.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450627200000
Organism	Drosophila pseudoobscura pseudoobscura
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001589954). On Dec 22, 2015 this sequence version replaced XM_001353043.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 3.03 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTATA TGGATGATTG CTTTGCCGGG GCCCCGCAAG ACTTTCTCAT CTGCATTACG 
GTGCACAAGG CCGCCAAAGT GGGCCTGGCC ACATCGGAGC TTTACGTCAA AGTCAGCCTG 
GATAAGACGC AGAAGAGCAC CAAGACGTTC CAAAACTCCG AGAATCCCTA CTTCAATGAG 
TACTTTGTGT TCGAGTTCCG CTGTTCGCTG AGCGACCTCC TCCGGATGAC CATCCTCTTT 
GAATTAAAGA AGACGCAGAC TCTCATGAAG AACCTGGTGG TCGGGGAGCT GTTGATCGAT 
CTGCATAGCG TGTGGAACCA GCCGAATCAC GGATTCTTCA AGAAGTGGGG TCGCCTGGAG 
CCGCCCATCG GGGAGAATCC TCCACGCGAG GAGCCCAGTG ATTCCCATGG GTATTTGCAA 
ATCGATCTGG TCATCGTCTC GCAGCACAGT TCGGTGGCAA ATAATCTCAT CCCAGAGGAG 
AACACCGACA CGCAGAGTCT GAACAAGTGG AGTGTGGACC AAGACTATGA CGACATTGAG 
AAGAACCTGC TGCAGAACCC CAACTCGTAC ACGCCGAGCA ACATCCGCTA TTCGATCGCC 
TTCTTCCGCG GCTTCTTCGT GCGGAAGGGC AACTACATGA TCCAATACAG ATTCCATCCT 
TTCAAGGGTA AGACGCCGGT GGCCAAGCAG ACCACGACGC CCGTGTGGAA CCACCAGATC 
AGTTTCGCCT GGATATACCC CTCGCTGGCG CAGCGCTTCC TCATCCTGGT CCACATCCAC 
GAGCACCTCA AATGGAAGTG CGTGGCCGAG TACGAGCTGT CATTCGAGGA GATCGCCTTC 
AAAAGCATTC CCTCTTTGGG ACCCACCTAT CTGCAATTGT ACGATCCCGT CCAGCCCATG 
ACCTATGTGG GTCGCCTCTT ACTGGAGGTG CGTTCCGACA CTGTGGCGGA GGAGAGGCCG 
TCCCATGTGA TCCTTGCGGA GGACGTTCAC GGCTTGGACG AGTCTCCTTT TTGGAAGGAA 
GAGGTCTTCA TGGTGGAGTT CCTTCCACTG CTGGGGGATC ACATCCAAAG CAGTGCCAGC 
AGCTATAAAA TCTCCATGAA GCTGGCCGAG AACACTTCCA CCGTCCTGGA GGGTCCCCTG 
AAGACGCCAT CGGGGAAGTT CCGCTCGGCG ATGCACTCGA AAAGCTTCCG CCAGGATGCC 
CCCTACCGAT CCTGCCTCCT ACGTGCCCGC CTGCCCGACA ATCGGGACAA GTACGAGAGC 
GACTACTTCA TGATCGACCT GGTCGACTTT ATGCGTTCCG AACTGGACAC CTTCAAGGTG 
TTCCAGCTGA AATCCCCGCA ACAGGACGTT AATCAGGCCA AGTGTCTGAA GGCCATCATA 
TCCAATATCT TGAGCAAAAT CAAGGAGGGC ATCAAGGAAC ATCGCTTCGA CCACGAGATG 
GAGAGCCAGC GCACTCCGTG GGACATCAAC CGCCAGCTTT TCCTCGTGGG ATTCTTCGCG 
AAGCTGCCGC TCGAGCTGCG TCAGCTGAAG CAGGAGCTCC GCAGCTCCTA CGTGGAGAAC 
CTGGACGCCT CTGTGGCGGA TGTGGTGAAC GAGCTGCATC GCCTGGTGGA CAAGATCACA 
GCGCTCTCCA ACATGACGCG GGTGCAGGAC GAGTGGCCCG ATCTCCTGCT CTCTCTAAGT 
GCCGGGGGCA AGGAGGTGGG CGTGTGCCGC CTCAATGCCA AGCTCTTTCT CCACCTCCAG 
CGCCGGGAGC TGCAGCAGAA CCAGATCACA CAGTGCTGGC GTCTAAAGAG CTTCGCGTTC 
AAGGACCCAG GTTGCATCCA CAGCTGCCCC AACTGCGGCT GCAGCACGGC CTTGATTCTG 
GGCTGCGTTG CCATCGTGGT CGAGAGGGAG CGCAAGCAGT TCCTCTCGAA CAGTATCGGT 
GACTGGATCT CGATCGAACC AATGGTCTGG AAGACCCGTG TGGCGTGGAC CTTCTTCCGC 
TGCCATGTGT ATGTCCATCA GGCCAAAGTC CGACCCACAG GAGAGAAGAA GCACGTCTGC 
GACTCCCATC TGCGCATCCT GTTCGGAGAG CAGGCCGCCG AGACGAACAC ATCCCCGGGC 
ACTCTGTCAC CCATCTGGGA TGCCGAAATC ACATTCAGCT GCATCACCCT GCCCGGCAGC 
GTCGACTGGT ACGTGCAGAA TCCCCCGCTG CTCTCCCTGG AGTTCTTCAA CAAGGAGAAA 
TCCCTCGCCG ATGAGCTGGT GGGCAAGGGC CATGTGATGG CCAGTGTGAT CAGTGGAGAA 
CATTTGGCGG ACTCCGCCTG GGAGGACGGT CCTCATTACT GGTCCAAGGG CCCCGTCAAC 
AAGATGCAGC GCATGAAGCA CGTCTCTCCG CCGCCCCTCA AGTGGGTGCC CCTGGCCAGG 
AAGGGTTCCG TCCAGGCGGA GGTCCTAATG TCGGCCGAGT TCATCGAGCT GGTGGATGGC 
GTTGCCGATG CCAGCAAGGA GCCCGTCCTA AGCACGGGTA TTCCGAAGGC CATCCGACCG 
AATATGCTGA ACTTTGTCCT GGAGGTTGTG TTCGTGGGGC TGCGCAACTA CTCCAAGACC 
ACCATGAGTA CGGCGGGCAA GCGAAAGATC AACATCATGA TGGGGGACCT GGTGCTCACG 
AGTGGTCCCT CGAAGGCCCG CATCGGCAAC AGCATCAACT TCCTCGTAGC CTACGCCTCA 
GCCGTTGTGA GTCTCCCCGA TCAGCAGGAG TACTGGCCAG CCATGATAGC CGCCGATGTG 
CTCGTCTCCG GGCTAACCAC AGAGTCCATA CTGGGGGCCG CACTCATACC GAACTCCGCC 
AGCTTCCTGC AGCGGGGAAA GACGACCAAA TGCGAGACCC AGAGCTCGGC TGCCTCCTAC 
ACCTCCACCG CAGTAACAAT GGATGAGGAA GAGGAGACTG AAGTGGATGC GACCGGGGGG 
AAGGACACAG AATGGGAGGA GGCCGATGAA GAGGAGGAGT CCCCCGGCAA GTGGAGGCGC 
TTATGGAGGC GGTTGATGTT CGCACTGGGC CTGCGACCCG CCGCCTATGC CAACAGGATG 
GCCCTGAGGT ATGCAGCGGA CGACATGGAC AACAGCGAGC TCATCGCGGA GGGACAGTTC 
ACGTGGTGGA CAAAGTTCTA CAACTCGATG TACTGGAAGG CGGCGGAGAT GACGCACGAG 
TACAAGCATC GGCTGGTCAT CTATCCCAAC GAGCTGGAGA AGCAGCCCCA GTTCGGCTAC 
CTGCAGGACT GGGCCGTGCC CGTCCAGATG GTGCACGGCG TCAAGTTCAG GAAGCAGGCC 
CCGCCGAGGG AGGATGTGTA CGCCACGCTG AAGATCCAGG TCAAGCTGAC GCCCTGCCAG 
TGCGGGAGCT GGGACGAAGG CGGAGGAGGG GACATGGTCC GACCCCTGGC AGCGTCCTTG 
AATCCGCGCG AACAGACGAT GCTCCAATCT CTGTCCGAGT CGGTGAGGCT AGCTGTCCGG 
GTCTATGTGG TGCAGGGCCT TCAGATGCGC CCGCGGGACG TCAAGCAAAA TTCCGACTGC 
TACGTGAAGC TCGCTCTTGG CGCCAAGACA CTCTCCGATC GGGCGGCGTA CATCCCTAAC 
CAGAGCAACC CCATCTTTGG GCGCATTTTC GAGCTGGAGG CCAACCTGCC CGGCGACAAC 
ATGCTCCTGC TGATGGTATA CGACCAGGAC ACATGCCGCG ACGATGTCAT CGGTCAGACG 
TACATCGATC TGGAGGATCG TTGGCGCAGC AAGCACCGTG CCACAGTGGG CCTGGCCCAG 
GAGTACTCGA GGAGTGGCTA CAACCAGTGG CACGATGTCA GCCTCCCATC GGAGATACTC 
ACGAGTATCT GCTTGCAACG GGGCATACAG CCGCCCTACT ACTACGGCAA CGTGCTCGAA 
GTAGATGGAC AGCTCTTCGC CGATGAAACA ACCATATCAC GGGGTGAGGA GATGCATGAA 
CGACTTAGTC TGACAGTGCT GAAAAACCTT GAGAGGTTGC CCTCCTTTGG CTACAAGCTG 
GTGCCCGAGC ATGTGGAGAC TCGCTCTCTC TACCGCGATG ATTATCCCAA TGTCGAACAG 
GGCAAGCTCC AGCTGTGGAT AGAGCTGTTT GAGGCCAACA AAGTCATGCC GTTTCCAGTT 
GATATCACGC CCGTGCCACC CGCTGGTTAC GAGGTCCGTG TTGTGGTCAA AAATCTGAGA 
GGCATCCAGG CGGGCGACAG AAATGTTTTT GGCAAACTAA TGAGCGACCT CTACGTAACT 
GGCTGGTGCC AGGATATAGA CAAGCGCCAA TCAACCGATA TCCATTACCG TTCGTTCACC 
GGCGATGCTG CCTTTAATTG GCGCATGATA TTCCCGATGA TGTACTCCCC CAATGAGGAC 
ATGATGGTCT TCCGCCGCCG AGGGGGACTG TTCGAGGAGA TTGAGGTCAA GCGACCACCG 
GTTATCTATC TGCAGATTTG GGACCGGGAC TTCATAACGC GCAACGAGTA CCTGGGCGCC 
CTGGAGCTGA ACCTCTCCAA CATGCCCATG CCGTATGAGA CGGAGGCGCA TTGCCAGGCG 
TACCCCAAAC AGCGGAAGCG ACTGAATCTC TTCCAGCGCA AGTCCATCCA TGGCTGGTTC 
CCAGTCGATA GTGCCCCGAA GCCGAGGTCT GGTCTGCCCA TCACCTCGAC GAAGGGCAAA 
ATGGAACTAC AAATCGATGT CTGTACGTCG GCGGAGGCCT CGAATCAACC CGCAGGTCAT 
GGCCATGACC CACCCTTGGC TTTGGAGAAG CCCGATCGGC CGGACACAGC CTTCAATCCG 
CTGACGCATC CCTTCAAGTC CTTCCACTAC ATCCTCTGGC CCTCCATACG GAAATATGTC 
CTGTGGGGCG TTCTCCTCTC GGTAGTTATC GTAGCAGTGG TTCTGTTCTT TACCAAAATC 
CCGAGCCAAC TCATGTCCAT TCCCTTTCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001353079.3
CDS	93..5045
Translation	MSYMDDCFAGAPQDFLICITVHKAAKVGLATSELYVKVSLDKTQKSTKTFQNSENPYFNEYFVFEFRCSLSDLLRMTILFELKKTQTLMKNLVVGELLIDLHSVWNQPNHGFFKKWGRLEPPIGENPPREEPSDSHGYLQIDLVIVSQHSSVANNLIPEENTDTQSLNKWSVDQDYDDIEKNLLQNPNSYTPSNIRYSIAFFRGFFVRKGNYMIQYRFHPFKGKTPVAKQTTTPVWNHQISFAWIYPSLAQRFLILVHIHEHLKWKCVAEYELSFEEIAFKSIPSLGPTYLQLYDPVQPMTYVGRLLLEVRSDTVAEERPSHVILAEDVHGLDESPFWKEEVFMVEFLPLLGDHIQSSASSYKISMKLAENTSTVLEGPLKTPSGKFRSAMHSKSFRQDAPYRSCLLRARLPDNRDKYESDYFMIDLVDFMRSELDTFKVFQLKSPQQDVNQAKCLKAIISNILSKIKEGIKEHRFDHEMESQRTPWDINRQLFLVGFFAKLPLELRQLKQELRSSYVENLDASVADVVNELHRLVDKITALSNMTRVQDEWPDLLLSLSAGGKEVGVCRLNAKLFLHLQRRELQQNQITQCWRLKSFAFKDPGCIHSCPNCGCSTALILGCVAIVVERERKQFLSNSIGDWISIEPMVWKTRVAWTFFRCHVYVHQAKVRPTGEKKHVCDSHLRILFGEQAAETNTSPGTLSPIWDAEITFSCITLPGSVDWYVQNPPLLSLEFFNKEKSLADELVGKGHVMASVISGEHLADSAWEDGPHYWSKGPVNKMQRMKHVSPPPLKWVPLARKGSVQAEVLMSAEFIELVDGVADASKEPVLSTGIPKAIRPNMLNFVLEVVFVGLRNYSKTTMSTAGKRKINIMMGDLVLTSGPSKARIGNSINFLVAYASAVVSLPDQQEYWPAMIAADVLVSGLTTESILGAALIPNSASFLQRGKTTKCETQSSAASYTSTAVTMDEEEETEVDATGGKDTEWEEADEEEESPGKWRRLWRRLMFALGLRPAAYANRMALRYAADDMDNSELIAEGQFTWWTKFYNSMYWKAAEMTHEYKHRLVIYPNELEKQPQFGYLQDWAVPVQMVHGVKFRKQAPPREDVYATLKIQVKLTPCQCGSWDEGGGGDMVRPLAASLNPREQTMLQSLSESVRLAVRVYVVQGLQMRPRDVKQNSDCYVKLALGAKTLSDRAAYIPNQSNPIFGRIFELEANLPGDNMLLLMVYDQDTCRDDVIGQTYIDLEDRWRSKHRATVGLAQEYSRSGYNQWHDVSLPSEILTSICLQRGIQPPYYYGNVLEVDGQLFADETTISRGEEMHERLSLTVLKNLERLPSFGYKLVPEHVETRSLYRDDYPNVEQGKLQLWIELFEANKVMPFPVDITPVPPAGYEVRVVVKNLRGIQAGDRNVFGKLMSDLYVTGWCQDIDKRQSTDIHYRSFTGDAAFNWRMIFPMMYSPNEDMMVFRRRGGLFEEIEVKRPPVIYLQIWDRDFITRNEYLGALELNLSNMPMPYETEAHCQAYPKQRKRLNLFQRKSIHGWFPVDSAPKPRSGLPITSTKGKMELQIDVCTSAEASNQPAGHGHDPPLALEKPDRPDTAFNPLTHPFKSFHYILWPSIRKYVLWGVLLSVVIVAVVLFFTKIPSQLMSIPFL

Target ORF information:

RefSeq Version	XM_001353043.3
Organism	Drosophila pseudoobscura pseudoobscura
Definition	Drosophila pseudoobscura pseudoobscura uncharacterized protein (Dpse\GA19103), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001353043.3

ORF Insert Sequence:

ATGAGTTATA TGGATGATTG CTTTGCCGGG GCCCCGCAAG ACTTTCTCAT CTGCATTACG 
GTGCACAAGG CCGCCAAAGT GGGCCTGGCC ACATCGGAGC TTTACGTCAA AGTCAGCCTG 
GATAAGACGC AGAAGAGCAC CAAGACGTTC CAAAACTCCG AGAATCCCTA CTTCAATGAG 
TACTTTGTGT TCGAGTTCCG CTGTTCGCTG AGCGACCTCC TCCGGATGAC CATCCTCTTT 
GAATTAAAGA AGACGCAGAC TCTCATGAAG AACCTGGTGG TCGGGGAGCT GTTGATCGAT 
CTGCATAGCG TGTGGAACCA GCCGAATCAC GGATTCTTCA AGAAGTGGGG TCGCCTGGAG 
CCGCCCATCG GGGAGAATCC TCCACGCGAG GAGCCCAGTG ATTCCCATGG GTATTTGCAA 
ATCGATCTGG TCATCGTCTC GCAGCACAGT TCGGTGGCAA ATAATCTCAT CCCAGAGGAG 
AACACCGACA CGCAGAGTCT GAACAAGTGG AGTGTGGACC AAGACTATGA CGACATTGAG 
AAGAACCTGC TGCAGAACCC CAACTCGTAC ACGCCGAGCA ACATCCGCTA TTCGATCGCC 
TTCTTCCGCG GCTTCTTCGT GCGGAAGGGC AACTACATGA TCCAATACAG ATTCCATCCT 
TTCAAGGGTA AGACGCCGGT GGCCAAGCAG ACCACGACGC CCGTGTGGAA CCACCAGATC 
AGTTTCGCCT GGATATACCC CTCGCTGGCG CAGCGCTTCC TCATCCTGGT CCACATCCAC 
GAGCACCTCA AATGGAAGTG CGTGGCCGAG TACGAGCTGT CATTCGAGGA GATCGCCTTC 
AAAAGCATTC CCTCTTTGGG ACCCACCTAT CTGCAATTGT ACGATCCCGT CCAGCCCATG 
ACCTATGTGG GTCGCCTCTT ACTGGAGGTG CGTTCCGACA CTGTGGCGGA GGAGAGGCCG 
TCCCATGTGA TCCTTGCGGA GGACGTTCAC GGCTTGGACG AGTCTCCTTT TTGGAAGGAA 
GAGGTCTTCA TGGTGGAGTT CCTTCCACTG CTGGGGGATC ACATCCAAAG CAGTGCCAGC 
AGCTATAAAA TCTCCATGAA GCTGGCCGAG AACACTTCCA CCGTCCTGGA GGGTCCCCTG 
AAGACGCCAT CGGGGAAGTT CCGCTCGGCG ATGCACTCGA AAAGCTTCCG CCAGGATGCC 
CCCTACCGAT CCTGCCTCCT ACGTGCCCGC CTGCCCGACA ATCGGGACAA GTACGAGAGC 
GACTACTTCA TGATCGACCT GGTCGACTTT ATGCGTTCCG AACTGGACAC CTTCAAGGTG 
TTCCAGCTGA AATCCCCGCA ACAGGACGTT AATCAGGCCA AGTGTCTGAA GGCCATCATA 
TCCAATATCT TGAGCAAAAT CAAGGAGGGC ATCAAGGAAC ATCGCTTCGA CCACGAGATG 
GAGAGCCAGC GCACTCCGTG GGACATCAAC CGCCAGCTTT TCCTCGTGGG ATTCTTCGCG 
AAGCTGCCGC TCGAGCTGCG TCAGCTGAAG CAGGAGCTCC GCAGCTCCTA CGTGGAGAAC 
CTGGACGCCT CTGTGGCGGA TGTGGTGAAC GAGCTGCATC GCCTGGTGGA CAAGATCACA 
GCGCTCTCCA ACATGACGCG GGTGCAGGAC GAGTGGCCCG ATCTCCTGCT CTCTCTAAGT 
GCCGGGGGCA AGGAGGTGGG CGTGTGCCGC CTCAATGCCA AGCTCTTTCT CCACCTCCAG 
CGCCGGGAGC TGCAGCAGAA CCAGATCACA CAGTGCTGGC GTCTAAAGAG CTTCGCGTTC 
AAGGACCCAG GTTGCATCCA CAGCTGCCCC AACTGCGGCT GCAGCACGGC CTTGATTCTG 
GGCTGCGTTG CCATCGTGGT CGAGAGGGAG CGCAAGCAGT TCCTCTCGAA CAGTATCGGT 
GACTGGATCT CGATCGAACC AATGGTCTGG AAGACCCGTG TGGCGTGGAC CTTCTTCCGC 
TGCCATGTGT ATGTCCATCA GGCCAAAGTC CGACCCACAG GAGAGAAGAA GCACGTCTGC 
GACTCCCATC TGCGCATCCT GTTCGGAGAG CAGGCCGCCG AGACGAACAC ATCCCCGGGC 
ACTCTGTCAC CCATCTGGGA TGCCGAAATC ACATTCAGCT GCATCACCCT GCCCGGCAGC 
GTCGACTGGT ACGTGCAGAA TCCCCCGCTG CTCTCCCTGG AGTTCTTCAA CAAGGAGAAA 
TCCCTCGCCG ATGAGCTGGT GGGCAAGGGC CATGTGATGG CCAGTGTGAT CAGTGGAGAA 
CATTTGGCGG ACTCCGCCTG GGAGGACGGT CCTCATTACT GGTCCAAGGG CCCCGTCAAC 
AAGATGCAGC GCATGAAGCA CGTCTCTCCG CCGCCCCTCA AGTGGGTGCC CCTGGCCAGG 
AAGGGTTCCG TCCAGGCGGA GGTCCTAATG TCGGCCGAGT TCATCGAGCT GGTGGATGGC 
GTTGCCGATG CCAGCAAGGA GCCCGTCCTA AGCACGGGTA TTCCGAAGGC CATCCGACCG 
AATATGCTGA ACTTTGTCCT GGAGGTTGTG TTCGTGGGGC TGCGCAACTA CTCCAAGACC 
ACCATGAGTA CGGCGGGCAA GCGAAAGATC AACATCATGA TGGGGGACCT GGTGCTCACG 
AGTGGTCCCT CGAAGGCCCG CATCGGCAAC AGCATCAACT TCCTCGTAGC CTACGCCTCA 
GCCGTTGTGA GTCTCCCCGA TCAGCAGGAG TACTGGCCAG CCATGATAGC CGCCGATGTG 
CTCGTCTCCG GGCTAACCAC AGAGTCCATA CTGGGGGCCG CACTCATACC GAACTCCGCC 
AGCTTCCTGC AGCGGGGAAA GACGACCAAA TGCGAGACCC AGAGCTCGGC TGCCTCCTAC 
ACCTCCACCG CAGTAACAAT GGATGAGGAA GAGGAGACTG AAGTGGATGC GACCGGGGGG 
AAGGACACAG AATGGGAGGA GGCCGATGAA GAGGAGGAGT CCCCCGGCAA GTGGAGGCGC 
TTATGGAGGC GGTTGATGTT CGCACTGGGC CTGCGACCCG CCGCCTATGC CAACAGGATG 
GCCCTGAGGT ATGCAGCGGA CGACATGGAC AACAGCGAGC TCATCGCGGA GGGACAGTTC 
ACGTGGTGGA CAAAGTTCTA CAACTCGATG TACTGGAAGG CGGCGGAGAT GACGCACGAG 
TACAAGCATC GGCTGGTCAT CTATCCCAAC GAGCTGGAGA AGCAGCCCCA GTTCGGCTAC 
CTGCAGGACT GGGCCGTGCC CGTCCAGATG GTGCACGGCG TCAAGTTCAG GAAGCAGGCC 
CCGCCGAGGG AGGATGTGTA CGCCACGCTG AAGATCCAGG TCAAGCTGAC GCCCTGCCAG 
TGCGGGAGCT GGGACGAAGG CGGAGGAGGG GACATGGTCC GACCCCTGGC AGCGTCCTTG 
AATCCGCGCG AACAGACGAT GCTCCAATCT CTGTCCGAGT CGGTGAGGCT AGCTGTCCGG 
GTCTATGTGG TGCAGGGCCT TCAGATGCGC CCGCGGGACG TCAAGCAAAA TTCCGACTGC 
TACGTGAAGC TCGCTCTTGG CGCCAAGACA CTCTCCGATC GGGCGGCGTA CATCCCTAAC 
CAGAGCAACC CCATCTTTGG GCGCATTTTC GAGCTGGAGG CCAACCTGCC CGGCGACAAC 
ATGCTCCTGC TGATGGTATA CGACCAGGAC ACATGCCGCG ACGATGTCAT CGGTCAGACG 
TACATCGATC TGGAGGATCG TTGGCGCAGC AAGCACCGTG CCACAGTGGG CCTGGCCCAG 
GAGTACTCGA GGAGTGGCTA CAACCAGTGG CACGATGTCA GCCTCCCATC GGAGATACTC 
ACGAGTATCT GCTTGCAACG GGGCATACAG CCGCCCTACT ACTACGGCAA CGTGCTCGAA 
GTAGATGGAC AGCTCTTCGC CGATGAAACA ACCATATCAC GGGGTGAGGA GATGCATGAA 
CGACTTAGTC TGACAGTGCT GAAAAACCTT GAGAGGTTGC CCTCCTTTGG CTACAAGCTG 
GTGCCCGAGC ATGTGGAGAC TCGCTCTCTC TACCGCGATG ATTATCCCAA TGTCGAACAG 
GGCAAGCTCC AGCTGTGGAT AGAGCTGTTT GAGGCCAACA AAGTCATGCC GTTTCCAGTT 
GATATCACGC CCGTGCCACC CGCTGGTTAC GAGGTCCGTG TTGTGGTCAA AAATCTGAGA 
GGCATCCAGG CGGGCGACAG AAATGTTTTT GGCAAACTAA TGAGCGACCT CTACGTAACT 
GGCTGGTGCC AGGATATAGA CAAGCGCCAA TCAACCGATA TCCATTACCG TTCGTTCACC 
GGCGATGCTG CCTTTAATTG GCGCATGATA TTCCCGATGA TGTACTCCCC CAATGAGGAC 
ATGATGGTCT TCCGCCGCCG AGGGGGACTG TTCGAGGAGA TTGAGGTCAA GCGACCACCG 
GTTATCTATC TGCAGATTTG GGACCGGGAC TTCATAACGC GCAACGAGTA CCTGGGCGCC 
CTGGAGCTGA ACCTCTCCAA CATGCCCATG CCGTATGAGA CGGAGGCGCA TTGCCAGGCG 
TACCCCAAAC AGCGGAAGCG ACTGAATCTC TTCCAGCGCA AGTCCATCCA TGGCTGGTTC 
CCAGTCGATA GTGCCCCGAA GCCGAGGTCT GGTCTGCCCA TCACCTCGAC GAAGGGCAAA 
ATGGAACTAC AAATCGATGT CTGTACGTCG GCGGAGGCCT CGAATCAACC CGCAGGTCAT 
GGCCATGACC CACCCTTGGC TTTGGAGAAG CCCGATCGGC CGGACACAGC CTTCAATCCG 
CTGACGCATC CCTTCAAGTC CTTCCACTAC ATCCTCTGGC CCTCCATACG GAAATATGTC 
CTGTGGGGCG TTCTCCTCTC GGTAGTTATC GTAGCAGTGG TTCTGTTCTT TACCAAAATC 
CCGAGCCAAC TCATGTCCAT TCCCTTTCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mind the gap: upgrading genomes with Pacific Biosciences RS long-read sequencing technology.
PloS one7(11)e47768(2012)
English AC,Richards S,Han Y,Wang M,Vee V,Qu J,Qin X,Muzny DM,Reid JG,Worley KC,Gibbs RA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution.
Genome research15(1)1-18(2005 Jan)
Richards S,Liu Y,Bettencourt BR,Hradecky P,Letovsky S,Nielsen R,Thornton K,Hubisz MJ,Chen R,Meisel RP,Couronne O,Hua S,Smith MA,Zhang P,Liu J,Bussemaker HJ,van Batenburg MF,Howells SL,Scherer SE,Sodergren E,Matthews BB,Crosby MA,Schroeder AJ,Ortiz-Barrientos D,Rives CM,Metzker ML,Muzny DM,Scott G,Steffen D,Wheeler DA,Worley KC,Havlak P,Durbin KJ,Egan A,Gill R,Hume J,Morgan MB,Miner G,Hamilton C,Huang Y,Waldron L,Verduzco D,Clerc-Blankenburg KP,Dubchak I,Noor MA,Anderson W,White KP,Clark AG,Schaeffer SW,Gelbart W,Weinstock GM,Gibbs RA

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Dpse\GA19103 cDNA ORF clone, Drosophila pseudoobscura pseudoobscura

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