Dere\GG22325 cDNA ORF clone, Drosophila erecta

Gene
Clones
gRNAs

Summary

Gene Symbol	Dere\GG22325
Entrez Gene ID	6548871
Full Name	GG22325 gene product from transcript GG22325-RA
Synonyms	GG22325,dere_GLEANR_7066
Gene Type	protein-coding
Organism	Drosophila erecta

mRNA and Protein(s)

mRNA	Protein	Name
XM_001975421.3	XP_001975457.2	LOW QUALITY PROTEIN: protein slit
XM_001975421.2	XP_001975457.2	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Assembly reconciliation.
Zimin AV, Smith DR, Sutton G, Yorke JA
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)

Evolution of genes and genomes on the Drosophila phylogeny.
, Clark AG, Eisen MB, Smith DR, Bergman CM, Oliver B, Markow TA, Kaufman TC, Kellis M, Gelbart W, Iyer VN, Pollard DA, Sackton TB, Larracuente AM, Singh ND, Abad JP, Abt DN, Adryan B, Aguade M, Akashi H, Anderson WW, Aquadro CF, Ardell DH, Arguello R, Artieri CG, Barbash DA, Barker D, Barsanti P, Batterham P, Batzoglou S, Begun D, Bhutkar A, Blanco E, Bosak SA, Bradley RK, Brand AD, Brent MR, Brooks AN, Brown RH, Butlin RK, Caggese C, Calvi BR, Bernardo de Carvalho A, Caspi A, Castrezana S, Celniker SE, Chang JL, Chapple C, Chatterji S, Chinwalla A, Civetta A, Clifton SW, Comeron JM, Costello JC, Coyne JA, Daub J, David RG, Delcher AL, Delehaunty K, Do CB, Ebling H, Edwards K, Eickbush T, Evans JD, Filipski A, Findeiss S, Freyhult E, Fulton L, Fulton R, Garcia AC, Gardiner A, Garfield DA, Garvin BE, Gibson G, Gilbert D, Gnerre S, Godfrey J, Good R, Gotea V, Gravely B, Greenberg AJ, Griffiths-Jones S, Gross S, Guigo R, Gustafson EA, Haerty W, Hahn MW, Halligan DL, Halpern AL, Halter GM, Han MV, Heger A, Hillier L, Hinrichs AS, Holmes I, Hoskins RA, Hubisz MJ, Hultmark D, Huntley MA, Jaffe DB, Jagadeeshan S, Jeck WR, Johnson J, Jones CD, Jordan WC, Karpen GH, Kataoka E, Keightley PD, Kheradpour P, Kirkness EF, Koerich LB, Kristiansen K, Kudrna D, Kulathinal RJ, Kumar S, Kwok R, Lander E, Langley CH, Lapoint R, Lazzaro BP, Lee SJ, Levesque L, Li R, Lin CF, Lin MF, Lindblad-Toh K, Llopart A, Long M, Low L, Lozovsky E, Lu J, Luo M, Machado CA, Makalowski W, Marzo M, Matsuda M, Matzkin L, McAllister B, McBride CS, McKernan B, McKernan K, Mendez-Lago M, Minx P, Mollenhauer MU, Montooth K, Mount SM, Mu X, Myers E, Negre B, Newfeld S, Nielsen R, Noor MA, O'Grady P, Pachter L, Papaceit M, Parisi MJ, Parisi M, Parts L, Pedersen JS, Pesole G, Phillippy AM, Ponting CP, Pop M, Porcelli D, Powell JR, Prohaska S, Pruitt K, Puig M, Quesneville H, Ram KR, Rand D, Rasmussen MD, Reed LK, Reenan R, Reily A, Remington KA, Rieger TT, Ritchie MG, Robin C, Rogers YH, Rohde C, Rozas J, Rubenfield MJ, Ruiz A, Russo S, Salzberg SL, Sanchez-Gracia A, Saranga DJ, Sato H, Schaeffer SW, Schatz MC, Schlenke T, Schwartz R, Segarra C, Singh RS, Sirot L, Sirota M, Sisneros NB, Smith CD, Smith TF, Spieth J, Stage DE, Stark A, Stephan W, Strausberg RL, Strempel S, Sturgill D, Sutton G, Sutton GG, Tao W, Teichmann S, Tobari YN, Tomimura Y, Tsolas JM, Valente VL, Venter E, Venter JC, Vicario S, Vieira FG, Vilella AJ, Villasante A, Walenz B, Wang J, Wasserman M, Watts T, Wilson D, Wilson RK, Wing RA, Wolfner MF, Wong A, Wong GK, Wu CI, Wu G, Yamamoto D, Yang HP, Yang SP, Yorke JA, Yoshida K, Zdobnov E, Zhang P, Zhang Y, Zimin AV, Baldwin J, Abdouelleil A, Abdulkadir J, Abebe A, Abera B, Abreu J, Acer SC, Aftuck L, Alexander A, An P, Anderson E, Anderson S, Arachi H, Azer M, Bachantsang P, Barry A, Bayul T, Berlin A, Bessette D, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Bourzgui I, Brown A, Cahill P, Channer S, Cheshatsang Y, Chuda L, Citroen M, Collymore A, Cooke P, Costello M, D'Aco K, Daza R, De Haan G, DeGray S, DeMaso C, Dhargay N, Dooley K, Dooley E, Doricent M, Dorje P, Dorjee K, Dupes A, Elong R, Falk J, Farina A, Faro S, Ferguson D, Fisher S, Foley CD, Franke A, Friedrich D, Gadbois L, Gearin G, Gearin CR, Giannoukos G, Goode T, Graham J, Grandbois E, Grewal S, Gyaltsen K, Hafez N, Hagos B, Hall J, Henson C, Hollinger A, Honan T, Huard MD, Hughes L, Hurhula B, Husby ME, Kamat A, Kanga B, Kashin S, Khazanovich D, Kisner P, Lance K, Lara M, Lee W, Lennon N, Letendre F, LeVine R, Lipovsky A, Liu X, Liu J, Liu S, Lokyitsang T, Lokyitsang Y, Lubonja R, Lui A, MacDonald P, Magnisalis V, Maru K, Matthews C, McCusker W, McDonough S, Mehta T, Meldrim J, Meneus L, Mihai O, Mihalev A, Mihova T, Mittelman R, Mlenga V, Montmayeur A, Mulrain L, Navidi A, Naylor J, Negash T, Nguyen T, Nguyen N, Nicol R, Norbu C, Norbu N, Novod N, O'Neill B, Osman S, Markiewicz E, Oyono OL, Patti C, Phunkhang P, Pierre F, Priest M, Raghuraman S, Rege F, Reyes R, Rise C, Rogov P, Ross K, Ryan E, Settipalli S, Shea T, Sherpa N, Shi L, Shih D, Sparrow T, Spaulding J, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Strader C, Tesfaye S, Thomson T, Thoulutsang Y, Thoulutsang D, Topham K, Topping I, Tsamla T, Vassiliev H, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Young G, Yu Q, Zembek L, Zhong D, Zimmer A, Zwirko Z, Jaffe DB, Alvarez P, Brockman W, Butler J, Chin C, Gnerre S, Grabherr M, Kleber M, Mauceli E, MacCallum I
Nature450(7167)203-18(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dere\GG22325 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dere\GG22325 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODi35792	XM_001975421.3 Latest version!	Drosophila erecta protein slit (LOC6548871), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
ODi35792	XM_001975421.2	Drosophila erecta uncharacterized protein (Dere\GG22325), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dere\GG22325 ( XM_001975421.3 ) cDNA ORF clone, Drosophila erecta -> XP_001975457.2 Drosophila erecta protein slit (LOC6548871), mRNA.

Price & Availability↑

CloneID	ODi35792
Clone ID Related Accession (Same CDS sequence)	XM_001975421.3 , XM_001975421.2
Accession Version	XM_001975421.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1541347200000
Organism	Drosophila erecta
Product	LOW QUALITY PROTEIN: protein slit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020825207.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 6, 2018 this sequence version replaced XM_001975421.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Drosophila erecta Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGCCGCGC TGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCCC CATGATGCGG TCCACGCGGA ACCGTACTCC 
GGCGGATTCG GCAGCTCAGC GGTATCCAGC GGTGGACTGG GGTCAGTGGG CATCCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACA 
GGATTAAATG TAGACTGCTC GCATCGAGGA CTCACCTCTG TGCCCAGGAA AATCTCTGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACGGACA ATCAAATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT AGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACCACCGT GGGAAGACGC GTCTTCAAAG GAGCCCAATC GCTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGT CTGGATGAGC ACGCTTTTAA AGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAATAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTCTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCCCG GTTCCTCCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGCGCAGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACCT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAACTT CATTACGGAA 
CTGCCGCCTA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATCTCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGTTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTCCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCAATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACCACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGCCGCC TGCCGCACTT GGTGAAGCTG 
GAGCTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG TGCATCCCAC 
ATCCAGGAGC TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TTTCAAATAA GATGTTCCTG 
GGACTCCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCGTG CGTTATGCCC 
GGTTCCTTTG AACATCTCAA CTCTCTGACG TCGCTGAATC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGTGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CCTGCCCCAC 
TCGGAATTCA AGTGCAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTT CGCTGTTCGC GCAACCAGCT CAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATCCG CCACCTGCGC TCCCTCACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTC GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CTCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAT 
CTCAAGTCGT TGACCCACAT AGCGCTGGGC AGCAATCCCT TGTACTGCGA CTGCGGCCTG 
AAGTGGTTCT CCGACTGGAT CAAGCTGGAC TACGTGGAGC CGGGAATTGC GCGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACGC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGTAACG CCTGTTTCGA GCAACCCTGC 
CAGAATCAGG CCCAATGCGT GGCCCTTCCT CAGCGGGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG CGAGTTTATG ATCGATGCCT GCTACGGCAA CCCGTGCCGC 
AACAATGCCA CTTGCACGGT ACTGGAGGAG GGTCGATTTA GCTGCCAGTG CGCTCCGGGC 
TACACAGGTG CCCGCTGCGA AACGAATATC GACGACTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CTTGTATCGA CGGAGTGGAG TCGTACAAGT GTGAGTGCCA GCCGGGATTC 
ACTGGCGAAT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CCGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATAGA CCACTTTACC CACTACAGCT GTGACTGTCA GGCAGGTTTC 
CATGGTACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGC 
GGAACGTGCG TGGACGGCAT CAATGACTAC CAATGTCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGTCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGCCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGATA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATCAGCTTC 
GTCCACAACA ACTCGTTTGT GGAACTCGAG CCACTGCGTA CGCGGCCGGA GGCGAACGTG 
ACGATAGTCT TCAGCAGCGC AGAGCAGAAT GGTATTCTCA TGTACGATGG ACAAGATGCT 
CACCTCGCCG TGGAGCTGTT TAATGGACGT ATTCGGGTTA GCTACGATGT GGGCAATCAC 
CCCGTATCCA CAATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCAGTGGAG 
CTGCTGGCCA TCAAGAAGAA TTTTACGCTG CGCGTGGATC GCGGACTGGC CCGCTCCATC 
ATCAACGAGG GCTCCAATGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGTCTA 
CCCGTGGATC CTGCACAGCA GGCATACAAG AACTGGCAGA TCCGCAACCT TACCAGCTTC 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAC CACAAGCTGG TGGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC TGGATGTGCC CTGCTCGAAG GAGAGCAGCA GGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACT CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAGTGCCG GCGCGGCAGT CGCTGCGTGC CGAATTCCAA TACCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTATACGG AGAACGACTG TCGCTCCAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAG ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGACTGAA AGATGCGACT ACCCAATTGC TCGAGCGGAG CAATAGCAGC 
TTAGATGTTA GTTTAGGAAC AGGTTTAAAT CTAACTTATA GTAGTAGTAA TAGTAACGAT 
AGTCTTAGCC ATAGCACTAG GGATAGCACG GATGTTGGGG GGACGAAGGG TGAAGTGGAG 
GAGAGTGCTG ACGCGGGGGA GACGACGGAG GAGGTGAGGG AGGAGAGTGA TGATTACCCC 
ACGGACGGCA TGCAGTCCGA TCTCTACGAC GAAACCATCG ATGACGACGA TGACGATGGT 
CTCGATGATG ATTATGCAGA TGAGGAGGAT GAGGAGGAGG ATCCAGAGCA GCTTCCCGAT 
CCCAAGGGTC TGGTAAGTGT GCCAGATGAA GAGGAAGACA TGGGCTACGA CGAGGATGAC 
GAGCGCATCG CCATGGAGCG GCCAAGAACG GTTAGACCCA GGCCCGATGA GGAGCATTTC 
CTCAACGAAG AGGGCAGTGG CTTTGGCGGA TTTCGATCGC GATTCCGGCC GAGCAACAGC 
TTCCGCGAAA CTCAGCTGGA GAATATCCGC AAGAAGCTGC TCACAGAGGC CCAGGCGGCT 
CCGGAAGCGG CAGTTGCAGT GGCCGTGCCG AGCACTGCGA TAGATCTGCG CGAGAGCAGC 
GGCCACTTTG CCAACGATGA CGAGGATGGC GAGGACGGCG ATGACGGCGT CGATGACGAG 
TTCGCCGACA CGGGGGAGAA CCAGGGGCGC GGCTTCTTTG GCTCCCAGCA GCAACAGCGC 
AAGAACGGTC CGTATCACCG CAAGAACGGC AACGATGCCA TTAAAATCAT CTCCACGCCG 
CTGGGAAAGG TGAGCATTGT GTACCAGCAG ACGGACAAGG ACCAAAGTCC GGACAAGGAT 
GCGCAACAGC AGCAGCAAAA GAAGCCAGCG CTTACCGACT TCGACGCCCT GTCACCGGAC 
CCCGAGAGCA GTCATCGCTT TCCGTCGCCC CACCCCAAGA TTACACCTGT TCTGACGCCC 
GATGGCAAGG TGGCGCTGCT CTATCGCGGA GACTCGGAGA GCTCCAAGTA CGAGCCCATT 
CGCAACCTGA CGCACAAGTT TTCCGGACAG CCGGCCAAGG AGCCAAAGCC TAAAACCGAA 
GACTCCTCCT CGGCGGAGGA CTCTGTCTAC ACGGACAGCG AGGATGCCGA GGACAGTAAA 
GGTGAAACGA GTGCTGGAAA GGCCCCCGCA GTGGCCAATA CGCCGAAGCC ACTGCAGCCA 
GAGATCTTGG AGCCTCCAGA TAACGTCCAG CCAGGAGGGT CCTTTATAAT TCGACCCACC 
TCCGACTCGC TTCTGCCGAT GATCAACAGG CCGCTGTCCG AGGTTCTCGG GATCAAGAAG 
AACCAGTTCC AGGAGACGCG GGTGCGCGAC CAACTGCCCA CGCAACAGCC TCCGCCTCCA 
CTGCCAGAGG CCACGTCCCG TAGTCCCGCT TCCGGACATC AGTTCCTAGC CAAGGTGAAC 
CTGGCTGAGT TCCCGACATC CGGAAGGACG CTCCAGACGC CGCTGATCCC CAGCACCCAC 
GACTTTGACT TCAGTCGTGA CAACACGATG CTGGACGAGC GGTCGCGGGT GCGTGAGTTA 
GAAAAGCAGC GGGAGCGGGA CAAGGAGCAC AACGAGGCCA CCAGCAAGGG AGCAACCGAG 
GCGCACACTA TAGCCAACGA GCAGCTGCTC TCCAAGACGG AGGTCATCAA TCTGGCCATT 
GTGCCACAGT TTGACGAGGA CTTGGAGCGT CTACAGCGGC TGCAGGAGAA TGGTGGTCGG 
CGCCACCACA GGGCGCGGCA TCGTCATCGG CAACAGTCGG AGGAGGAACT GTCTGGCATC 
CATTGCATCA TGCAGGTCAT GATGGGCGTG GCCGCCGTGT CGACGGTCTT CGGCATGCTG 
GGCACCTTCT TCAAGCAACG CATCCTCGAT CAGCTGCGCA TGATGCACTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001975457.2
CDS	679..7152
Translation	MAALSRTTLMPPPFRLQLRLLILPILLLLPHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENLVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFTGEFCDTKIQFCSPEFNPCANGAKCIDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNTRDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY*LKDATTQLLERSNSSLDVSLGTGLNLTYSSSNSNDSLSHSTRDSTDVGGTKGEVEESADAGETTEEVREESDDYPTDGMQSDLYDETIDDDDDDGLDDDYADEEDEEEDPEQLPDPKGLVSVPDEEEDMGYDEDDERIAMERPRTVRPRPDEEHFLNEEGSGFGGFRSRFRPSNSFRETQLENIRKKLLTEAQAAPEAAVAVAVPSTAIDLRESSGHFANDDEDGEDGDDGVDDEFADTGENQGRGFFGSQQQQRKNGPYHRKNGNDAIKIISTPLGKVSIVYQQTDKDQSPDKDAQQQQQKKPALTDFDALSPDPESSHRFPSPHPKITPVLTPDGKVALLYRGDSESSKYEPIRNLTHKFSGQPAKEPKPKTEDSSSAEDSVYTDSEDAEDSKGETSAGKAPAVANTPKPLQPEILEPPDNVQPGGSFIIRPTSDSLLPMINRPLSEVLGIKKNQFQETRVRDQLPTQQPPPPLPEATSRSPASGHQFLAKVNLAEFPTSGRTLQTPLIPSTHDFDFSRDNTMLDERSRVRELEKQRERDKEHNEATSKGATEAHTIANEQLLSKTEVINLAIVPQFDEDLERLQRLQENGGRRHHRARHRHRQQSEEELSGIHCIMQVMMGVAAVSTVFGMLGTFFKQRILDQLRMMHW

Target ORF information:

RefSeq Version	XM_001975421.3
Organism	Drosophila erecta
Definition	Drosophila erecta protein slit (LOC6548871), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001975421.3

ORF Insert Sequence:

ATGGCCGCGC TGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCCC CATGATGCGG TCCACGCGGA ACCGTACTCC 
GGCGGATTCG GCAGCTCAGC GGTATCCAGC GGTGGACTGG GGTCAGTGGG CATCCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACA 
GGATTAAATG TAGACTGCTC GCATCGAGGA CTCACCTCTG TGCCCAGGAA AATCTCTGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACGGACA ATCAAATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT AGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACCACCGT GGGAAGACGC GTCTTCAAAG GAGCCCAATC GCTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGT CTGGATGAGC ACGCTTTTAA AGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAATAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTCTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCCCG GTTCCTCCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGCGCAGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACCT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAACTT CATTACGGAA 
CTGCCGCCTA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATCTCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGTTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTCCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCAATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACCACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGCCGCC TGCCGCACTT GGTGAAGCTG 
GAGCTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG TGCATCCCAC 
ATCCAGGAGC TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TTTCAAATAA GATGTTCCTG 
GGACTCCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCGTG CGTTATGCCC 
GGTTCCTTTG AACATCTCAA CTCTCTGACG TCGCTGAATC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGTGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CCTGCCCCAC 
TCGGAATTCA AGTGCAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTT CGCTGTTCGC GCAACCAGCT CAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATCCG CCACCTGCGC TCCCTCACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTC GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CTCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAT 
CTCAAGTCGT TGACCCACAT AGCGCTGGGC AGCAATCCCT TGTACTGCGA CTGCGGCCTG 
AAGTGGTTCT CCGACTGGAT CAAGCTGGAC TACGTGGAGC CGGGAATTGC GCGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACGC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGTAACG CCTGTTTCGA GCAACCCTGC 
CAGAATCAGG CCCAATGCGT GGCCCTTCCT CAGCGGGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG CGAGTTTATG ATCGATGCCT GCTACGGCAA CCCGTGCCGC 
AACAATGCCA CTTGCACGGT ACTGGAGGAG GGTCGATTTA GCTGCCAGTG CGCTCCGGGC 
TACACAGGTG CCCGCTGCGA AACGAATATC GACGACTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CTTGTATCGA CGGAGTGGAG TCGTACAAGT GTGAGTGCCA GCCGGGATTC 
ACTGGCGAAT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CCGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATAGA CCACTTTACC CACTACAGCT GTGACTGTCA GGCAGGTTTC 
CATGGTACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGC 
GGAACGTGCG TGGACGGCAT CAATGACTAC CAATGTCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGTCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGCCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGATA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATCAGCTTC 
GTCCACAACA ACTCGTTTGT GGAACTCGAG CCACTGCGTA CGCGGCCGGA GGCGAACGTG 
ACGATAGTCT TCAGCAGCGC AGAGCAGAAT GGTATTCTCA TGTACGATGG ACAAGATGCT 
CACCTCGCCG TGGAGCTGTT TAATGGACGT ATTCGGGTTA GCTACGATGT GGGCAATCAC 
CCCGTATCCA CAATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCAGTGGAG 
CTGCTGGCCA TCAAGAAGAA TTTTACGCTG CGCGTGGATC GCGGACTGGC CCGCTCCATC 
ATCAACGAGG GCTCCAATGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGTCTA 
CCCGTGGATC CTGCACAGCA GGCATACAAG AACTGGCAGA TCCGCAACCT TACCAGCTTC 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAC CACAAGCTGG TGGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC TGGATGTGCC CTGCTCGAAG GAGAGCAGCA GGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACT CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAGTGCCG GCGCGGCAGT CGCTGCGTGC CGAATTCCAA TACCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTATACGG AGAACGACTG TCGCTCCAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAG ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGACTGAA AGATGCGACT ACCCAATTGC TCGAGCGGAG CAATAGCAGC 
TTAGATGTTA GTTTAGGAAC AGGTTTAAAT CTAACTTATA GTAGTAGTAA TAGTAACGAT 
AGTCTTAGCC ATAGCACTAG GGATAGCACG GATGTTGGGG GGACGAAGGG TGAAGTGGAG 
GAGAGTGCTG ACGCGGGGGA GACGACGGAG GAGGTGAGGG AGGAGAGTGA TGATTACCCC 
ACGGACGGCA TGCAGTCCGA TCTCTACGAC GAAACCATCG ATGACGACGA TGACGATGGT 
CTCGATGATG ATTATGCAGA TGAGGAGGAT GAGGAGGAGG ATCCAGAGCA GCTTCCCGAT 
CCCAAGGGTC TGGTAAGTGT GCCAGATGAA GAGGAAGACA TGGGCTACGA CGAGGATGAC 
GAGCGCATCG CCATGGAGCG GCCAAGAACG GTTAGACCCA GGCCCGATGA GGAGCATTTC 
CTCAACGAAG AGGGCAGTGG CTTTGGCGGA TTTCGATCGC GATTCCGGCC GAGCAACAGC 
TTCCGCGAAA CTCAGCTGGA GAATATCCGC AAGAAGCTGC TCACAGAGGC CCAGGCGGCT 
CCGGAAGCGG CAGTTGCAGT GGCCGTGCCG AGCACTGCGA TAGATCTGCG CGAGAGCAGC 
GGCCACTTTG CCAACGATGA CGAGGATGGC GAGGACGGCG ATGACGGCGT CGATGACGAG 
TTCGCCGACA CGGGGGAGAA CCAGGGGCGC GGCTTCTTTG GCTCCCAGCA GCAACAGCGC 
AAGAACGGTC CGTATCACCG CAAGAACGGC AACGATGCCA TTAAAATCAT CTCCACGCCG 
CTGGGAAAGG TGAGCATTGT GTACCAGCAG ACGGACAAGG ACCAAAGTCC GGACAAGGAT 
GCGCAACAGC AGCAGCAAAA GAAGCCAGCG CTTACCGACT TCGACGCCCT GTCACCGGAC 
CCCGAGAGCA GTCATCGCTT TCCGTCGCCC CACCCCAAGA TTACACCTGT TCTGACGCCC 
GATGGCAAGG TGGCGCTGCT CTATCGCGGA GACTCGGAGA GCTCCAAGTA CGAGCCCATT 
CGCAACCTGA CGCACAAGTT TTCCGGACAG CCGGCCAAGG AGCCAAAGCC TAAAACCGAA 
GACTCCTCCT CGGCGGAGGA CTCTGTCTAC ACGGACAGCG AGGATGCCGA GGACAGTAAA 
GGTGAAACGA GTGCTGGAAA GGCCCCCGCA GTGGCCAATA CGCCGAAGCC ACTGCAGCCA 
GAGATCTTGG AGCCTCCAGA TAACGTCCAG CCAGGAGGGT CCTTTATAAT TCGACCCACC 
TCCGACTCGC TTCTGCCGAT GATCAACAGG CCGCTGTCCG AGGTTCTCGG GATCAAGAAG 
AACCAGTTCC AGGAGACGCG GGTGCGCGAC CAACTGCCCA CGCAACAGCC TCCGCCTCCA 
CTGCCAGAGG CCACGTCCCG TAGTCCCGCT TCCGGACATC AGTTCCTAGC CAAGGTGAAC 
CTGGCTGAGT TCCCGACATC CGGAAGGACG CTCCAGACGC CGCTGATCCC CAGCACCCAC 
GACTTTGACT TCAGTCGTGA CAACACGATG CTGGACGAGC GGTCGCGGGT GCGTGAGTTA 
GAAAAGCAGC GGGAGCGGGA CAAGGAGCAC AACGAGGCCA CCAGCAAGGG AGCAACCGAG 
GCGCACACTA TAGCCAACGA GCAGCTGCTC TCCAAGACGG AGGTCATCAA TCTGGCCATT 
GTGCCACAGT TTGACGAGGA CTTGGAGCGT CTACAGCGGC TGCAGGAGAA TGGTGGTCGG 
CGCCACCACA GGGCGCGGCA TCGTCATCGG CAACAGTCGG AGGAGGAACT GTCTGGCATC 
CATTGCATCA TGCAGGTCAT GATGGGCGTG GCCGCCGTGT CGACGGTCTT CGGCATGCTG 
GGCACCTTCT TCAAGCAACG CATCCTCGAT CAGCTGCGCA TGATGCACTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dere\GG22325 ( XM_001975421.2 ) cDNA ORF clone, Drosophila erecta -> XP_001975457.2 Drosophila erecta uncharacterized protein (Dere\GG22325), mRNA.

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CloneID	ODi35792
Clone ID Related Accession (Same CDS sequence)	XM_001975421.3 , XM_001975421.2
Accession Version	XM_001975421.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1450540800000
Organism	Drosophila erecta
Product	uncharacterized protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001956550). On Dec 21, 2015 this sequence version replaced XM_001975421.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 1.04 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC TGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCCC CATGATGCGG TCCACGCGGA ACCGTACTCC 
GGCGGATTCG GCAGCTCAGC GGTATCCAGC GGTGGACTGG GGTCAGTGGG CATCCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACA 
GGATTAAATG TAGACTGCTC GCATCGAGGA CTCACCTCTG TGCCCAGGAA AATCTCTGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACGGACA ATCAAATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT AGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACCACCGT GGGAAGACGC GTCTTCAAAG GAGCCCAATC GCTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGT CTGGATGAGC ACGCTTTTAA AGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAATAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTCTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCCCG GTTCCTCCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGCGCAGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACCT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAACTT CATTACGGAA 
CTGCCGCCTA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATCTCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGTTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTCCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCAATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACCACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGCCGCC TGCCGCACTT GGTGAAGCTG 
GAGCTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG TGCATCCCAC 
ATCCAGGAGC TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TTTCAAATAA GATGTTCCTG 
GGACTCCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCGTG CGTTATGCCC 
GGTTCCTTTG AACATCTCAA CTCTCTGACG TCGCTGAATC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGTGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CCTGCCCCAC 
TCGGAATTCA AGTGCAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTT CGCTGTTCGC GCAACCAGCT CAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATCCG CCACCTGCGC TCCCTCACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTC GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CTCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAT 
CTCAAGTCGT TGACCCACAT AGCGCTGGGC AGCAATCCCT TGTACTGCGA CTGCGGCCTG 
AAGTGGTTCT CCGACTGGAT CAAGCTGGAC TACGTGGAGC CGGGAATTGC GCGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACGC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGTAACG CCTGTTTCGA GCAACCCTGC 
CAGAATCAGG CCCAATGCGT GGCCCTTCCT CAGCGGGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG CGAGTTTATG ATCGATGCCT GCTACGGCAA CCCGTGCCGC 
AACAATGCCA CTTGCACGGT ACTGGAGGAG GGTCGATTTA GCTGCCAGTG CGCTCCGGGC 
TACACAGGTG CCCGCTGCGA AACGAATATC GACGACTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CTTGTATCGA CGGAGTGGAG TCGTACAAGT GTGAGTGCCA GCCGGGATTC 
ACTGGCGAAT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CCGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATAGA CCACTTTACC CACTACAGCT GTGACTGTCA GGCAGGTTTC 
CATGGTACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGC 
GGAACGTGCG TGGACGGCAT CAATGACTAC CAATGTCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGTCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGCCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGATA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATCAGCTTC 
GTCCACAACA ACTCGTTTGT GGAACTCGAG CCACTGCGTA CGCGGCCGGA GGCGAACGTG 
ACGATAGTCT TCAGCAGCGC AGAGCAGAAT GGTATTCTCA TGTACGATGG ACAAGATGCT 
CACCTCGCCG TGGAGCTGTT TAATGGACGT ATTCGGGTTA GCTACGATGT GGGCAATCAC 
CCCGTATCCA CAATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCAGTGGAG 
CTGCTGGCCA TCAAGAAGAA TTTTACGCTG CGCGTGGATC GCGGACTGGC CCGCTCCATC 
ATCAACGAGG GCTCCAATGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGTCTA 
CCCGTGGATC CTGCACAGCA GGCATACAAG AACTGGCAGA TCCGCAACCT TACCAGCTTC 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAC CACAAGCTGG TGGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC TGGATGTGCC CTGCTCGAAG GAGAGCAGCA GGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACT CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAGTGCCG GCGCGGCAGT CGCTGCGTGC CGAATTCCAA TACCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTATACGG AGAACGACTG TCGCTCCAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAG ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGACTGAA AGATGCGACT ACCCAATTGC TCGAGCGGAG CAATAGCAGC 
TTAGATGTTA GTTTAGGAAC AGGTTTAAAT CTAACTTATA GTAGTAGTAA TAGTAACGAT 
AGTCTTAGCC ATAGCACTAG GGATAGCACG GATGTTGGGG GGACGAAGGG TGAAGTGGAG 
GAGAGTGCTG ACGCGGGGGA GACGACGGAG GAGGTGAGGG AGGAGAGTGA TGATTACCCC 
ACGGACGGCA TGCAGTCCGA TCTCTACGAC GAAACCATCG ATGACGACGA TGACGATGGT 
CTCGATGATG ATTATGCAGA TGAGGAGGAT GAGGAGGAGG ATCCAGAGCA GCTTCCCGAT 
CCCAAGGGTC TGGTAAGTGT GCCAGATGAA GAGGAAGACA TGGGCTACGA CGAGGATGAC 
GAGCGCATCG CCATGGAGCG GCCAAGAACG GTTAGACCCA GGCCCGATGA GGAGCATTTC 
CTCAACGAAG AGGGCAGTGG CTTTGGCGGA TTTCGATCGC GATTCCGGCC GAGCAACAGC 
TTCCGCGAAA CTCAGCTGGA GAATATCCGC AAGAAGCTGC TCACAGAGGC CCAGGCGGCT 
CCGGAAGCGG CAGTTGCAGT GGCCGTGCCG AGCACTGCGA TAGATCTGCG CGAGAGCAGC 
GGCCACTTTG CCAACGATGA CGAGGATGGC GAGGACGGCG ATGACGGCGT CGATGACGAG 
TTCGCCGACA CGGGGGAGAA CCAGGGGCGC GGCTTCTTTG GCTCCCAGCA GCAACAGCGC 
AAGAACGGTC CGTATCACCG CAAGAACGGC AACGATGCCA TTAAAATCAT CTCCACGCCG 
CTGGGAAAGG TGAGCATTGT GTACCAGCAG ACGGACAAGG ACCAAAGTCC GGACAAGGAT 
GCGCAACAGC AGCAGCAAAA GAAGCCAGCG CTTACCGACT TCGACGCCCT GTCACCGGAC 
CCCGAGAGCA GTCATCGCTT TCCGTCGCCC CACCCCAAGA TTACACCTGT TCTGACGCCC 
GATGGCAAGG TGGCGCTGCT CTATCGCGGA GACTCGGAGA GCTCCAAGTA CGAGCCCATT 
CGCAACCTGA CGCACAAGTT TTCCGGACAG CCGGCCAAGG AGCCAAAGCC TAAAACCGAA 
GACTCCTCCT CGGCGGAGGA CTCTGTCTAC ACGGACAGCG AGGATGCCGA GGACAGTAAA 
GGTGAAACGA GTGCTGGAAA GGCCCCCGCA GTGGCCAATA CGCCGAAGCC ACTGCAGCCA 
GAGATCTTGG AGCCTCCAGA TAACGTCCAG CCAGGAGGGT CCTTTATAAT TCGACCCACC 
TCCGACTCGC TTCTGCCGAT GATCAACAGG CCGCTGTCCG AGGTTCTCGG GATCAAGAAG 
AACCAGTTCC AGGAGACGCG GGTGCGCGAC CAACTGCCCA CGCAACAGCC TCCGCCTCCA 
CTGCCAGAGG CCACGTCCCG TAGTCCCGCT TCCGGACATC AGTTCCTAGC CAAGGTGAAC 
CTGGCTGAGT TCCCGACATC CGGAAGGACG CTCCAGACGC CGCTGATCCC CAGCACCCAC 
GACTTTGACT TCAGTCGTGA CAACACGATG CTGGACGAGC GGTCGCGGGT GCGTGAGTTA 
GAAAAGCAGC GGGAGCGGGA CAAGGAGCAC AACGAGGCCA CCAGCAAGGG AGCAACCGAG 
GCGCACACTA TAGCCAACGA GCAGCTGCTC TCCAAGACGG AGGTCATCAA TCTGGCCATT 
GTGCCACAGT TTGACGAGGA CTTGGAGCGT CTACAGCGGC TGCAGGAGAA TGGTGGTCGG 
CGCCACCACA GGGCGCGGCA TCGTCATCGG CAACAGTCGG AGGAGGAACT GTCTGGCATC 
CATTGCATCA TGCAGGTCAT GATGGGCGTG GCCGCCGTGT CGACGGTCTT CGGCATGCTG 
GGCACCTTCT TCAAGCAACG CATCCTCGAT CAGCTGCGCA TGATGCACTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001975457.2
CDS	33..6506
Translation	MAALSRTTLMPPPFRLQLRLLILPILLLLPHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENLVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFTGEFCDTKIQFCSPEFNPCANGAKCIDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNTRDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY*LKDATTQLLERSNSSLDVSLGTGLNLTYSSSNSNDSLSHSTRDSTDVGGTKGEVEESADAGETTEEVREESDDYPTDGMQSDLYDETIDDDDDDGLDDDYADEEDEEEDPEQLPDPKGLVSVPDEEEDMGYDEDDERIAMERPRTVRPRPDEEHFLNEEGSGFGGFRSRFRPSNSFRETQLENIRKKLLTEAQAAPEAAVAVAVPSTAIDLRESSGHFANDDEDGEDGDDGVDDEFADTGENQGRGFFGSQQQQRKNGPYHRKNGNDAIKIISTPLGKVSIVYQQTDKDQSPDKDAQQQQQKKPALTDFDALSPDPESSHRFPSPHPKITPVLTPDGKVALLYRGDSESSKYEPIRNLTHKFSGQPAKEPKPKTEDSSSAEDSVYTDSEDAEDSKGETSAGKAPAVANTPKPLQPEILEPPDNVQPGGSFIIRPTSDSLLPMINRPLSEVLGIKKNQFQETRVRDQLPTQQPPPPLPEATSRSPASGHQFLAKVNLAEFPTSGRTLQTPLIPSTHDFDFSRDNTMLDERSRVRELEKQRERDKEHNEATSKGATEAHTIANEQLLSKTEVINLAIVPQFDEDLERLQRLQENGGRRHHRARHRHRQQSEEELSGIHCIMQVMMGVAAVSTVFGMLGTFFKQRILDQLRMMHW

Target ORF information:

RefSeq Version	XM_001975421.2
Organism	Drosophila erecta
Definition	Drosophila erecta uncharacterized protein (Dere\GG22325), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001975421.2

ORF Insert Sequence:

ATGGCCGCGC TGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCCC CATGATGCGG TCCACGCGGA ACCGTACTCC 
GGCGGATTCG GCAGCTCAGC GGTATCCAGC GGTGGACTGG GGTCAGTGGG CATCCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACA 
GGATTAAATG TAGACTGCTC GCATCGAGGA CTCACCTCTG TGCCCAGGAA AATCTCTGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACGGACA ATCAAATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT AGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACCACCGT GGGAAGACGC GTCTTCAAAG GAGCCCAATC GCTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGT CTGGATGAGC ACGCTTTTAA AGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAATAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTCTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCCCG GTTCCTCCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGCGCAGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACCT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAACTT CATTACGGAA 
CTGCCGCCTA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATCTCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGTTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTCCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCAATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACCACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGCCGCC TGCCGCACTT GGTGAAGCTG 
GAGCTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG TGCATCCCAC 
ATCCAGGAGC TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TTTCAAATAA GATGTTCCTG 
GGACTCCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCGTG CGTTATGCCC 
GGTTCCTTTG AACATCTCAA CTCTCTGACG TCGCTGAATC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGTGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CCTGCCCCAC 
TCGGAATTCA AGTGCAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTT CGCTGTTCGC GCAACCAGCT CAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATCCG CCACCTGCGC TCCCTCACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTC GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CTCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAT 
CTCAAGTCGT TGACCCACAT AGCGCTGGGC AGCAATCCCT TGTACTGCGA CTGCGGCCTG 
AAGTGGTTCT CCGACTGGAT CAAGCTGGAC TACGTGGAGC CGGGAATTGC GCGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACGC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGTAACG CCTGTTTCGA GCAACCCTGC 
CAGAATCAGG CCCAATGCGT GGCCCTTCCT CAGCGGGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG CGAGTTTATG ATCGATGCCT GCTACGGCAA CCCGTGCCGC 
AACAATGCCA CTTGCACGGT ACTGGAGGAG GGTCGATTTA GCTGCCAGTG CGCTCCGGGC 
TACACAGGTG CCCGCTGCGA AACGAATATC GACGACTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CTTGTATCGA CGGAGTGGAG TCGTACAAGT GTGAGTGCCA GCCGGGATTC 
ACTGGCGAAT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CCGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATAGA CCACTTTACC CACTACAGCT GTGACTGTCA GGCAGGTTTC 
CATGGTACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGC 
GGAACGTGCG TGGACGGCAT CAATGACTAC CAATGTCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGTCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGCCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGATA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATCAGCTTC 
GTCCACAACA ACTCGTTTGT GGAACTCGAG CCACTGCGTA CGCGGCCGGA GGCGAACGTG 
ACGATAGTCT TCAGCAGCGC AGAGCAGAAT GGTATTCTCA TGTACGATGG ACAAGATGCT 
CACCTCGCCG TGGAGCTGTT TAATGGACGT ATTCGGGTTA GCTACGATGT GGGCAATCAC 
CCCGTATCCA CAATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCAGTGGAG 
CTGCTGGCCA TCAAGAAGAA TTTTACGCTG CGCGTGGATC GCGGACTGGC CCGCTCCATC 
ATCAACGAGG GCTCCAATGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGTCTA 
CCCGTGGATC CTGCACAGCA GGCATACAAG AACTGGCAGA TCCGCAACCT TACCAGCTTC 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAC CACAAGCTGG TGGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC TGGATGTGCC CTGCTCGAAG GAGAGCAGCA GGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACT CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAGTGCCG GCGCGGCAGT CGCTGCGTGC CGAATTCCAA TACCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTATACGG AGAACGACTG TCGCTCCAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAG ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGACTGAA AGATGCGACT ACCCAATTGC TCGAGCGGAG CAATAGCAGC 
TTAGATGTTA GTTTAGGAAC AGGTTTAAAT CTAACTTATA GTAGTAGTAA TAGTAACGAT 
AGTCTTAGCC ATAGCACTAG GGATAGCACG GATGTTGGGG GGACGAAGGG TGAAGTGGAG 
GAGAGTGCTG ACGCGGGGGA GACGACGGAG GAGGTGAGGG AGGAGAGTGA TGATTACCCC 
ACGGACGGCA TGCAGTCCGA TCTCTACGAC GAAACCATCG ATGACGACGA TGACGATGGT 
CTCGATGATG ATTATGCAGA TGAGGAGGAT GAGGAGGAGG ATCCAGAGCA GCTTCCCGAT 
CCCAAGGGTC TGGTAAGTGT GCCAGATGAA GAGGAAGACA TGGGCTACGA CGAGGATGAC 
GAGCGCATCG CCATGGAGCG GCCAAGAACG GTTAGACCCA GGCCCGATGA GGAGCATTTC 
CTCAACGAAG AGGGCAGTGG CTTTGGCGGA TTTCGATCGC GATTCCGGCC GAGCAACAGC 
TTCCGCGAAA CTCAGCTGGA GAATATCCGC AAGAAGCTGC TCACAGAGGC CCAGGCGGCT 
CCGGAAGCGG CAGTTGCAGT GGCCGTGCCG AGCACTGCGA TAGATCTGCG CGAGAGCAGC 
GGCCACTTTG CCAACGATGA CGAGGATGGC GAGGACGGCG ATGACGGCGT CGATGACGAG 
TTCGCCGACA CGGGGGAGAA CCAGGGGCGC GGCTTCTTTG GCTCCCAGCA GCAACAGCGC 
AAGAACGGTC CGTATCACCG CAAGAACGGC AACGATGCCA TTAAAATCAT CTCCACGCCG 
CTGGGAAAGG TGAGCATTGT GTACCAGCAG ACGGACAAGG ACCAAAGTCC GGACAAGGAT 
GCGCAACAGC AGCAGCAAAA GAAGCCAGCG CTTACCGACT TCGACGCCCT GTCACCGGAC 
CCCGAGAGCA GTCATCGCTT TCCGTCGCCC CACCCCAAGA TTACACCTGT TCTGACGCCC 
GATGGCAAGG TGGCGCTGCT CTATCGCGGA GACTCGGAGA GCTCCAAGTA CGAGCCCATT 
CGCAACCTGA CGCACAAGTT TTCCGGACAG CCGGCCAAGG AGCCAAAGCC TAAAACCGAA 
GACTCCTCCT CGGCGGAGGA CTCTGTCTAC ACGGACAGCG AGGATGCCGA GGACAGTAAA 
GGTGAAACGA GTGCTGGAAA GGCCCCCGCA GTGGCCAATA CGCCGAAGCC ACTGCAGCCA 
GAGATCTTGG AGCCTCCAGA TAACGTCCAG CCAGGAGGGT CCTTTATAAT TCGACCCACC 
TCCGACTCGC TTCTGCCGAT GATCAACAGG CCGCTGTCCG AGGTTCTCGG GATCAAGAAG 
AACCAGTTCC AGGAGACGCG GGTGCGCGAC CAACTGCCCA CGCAACAGCC TCCGCCTCCA 
CTGCCAGAGG CCACGTCCCG TAGTCCCGCT TCCGGACATC AGTTCCTAGC CAAGGTGAAC 
CTGGCTGAGT TCCCGACATC CGGAAGGACG CTCCAGACGC CGCTGATCCC CAGCACCCAC 
GACTTTGACT TCAGTCGTGA CAACACGATG CTGGACGAGC GGTCGCGGGT GCGTGAGTTA 
GAAAAGCAGC GGGAGCGGGA CAAGGAGCAC AACGAGGCCA CCAGCAAGGG AGCAACCGAG 
GCGCACACTA TAGCCAACGA GCAGCTGCTC TCCAAGACGG AGGTCATCAA TCTGGCCATT 
GTGCCACAGT TTGACGAGGA CTTGGAGCGT CTACAGCGGC TGCAGGAGAA TGGTGGTCGG 
CGCCACCACA GGGCGCGGCA TCGTCATCGG CAACAGTCGG AGGAGGAACT GTCTGGCATC 
CATTGCATCA TGCAGGTCAT GATGGGCGTG GCCGCCGTGT CGACGGTCTT CGGCATGCTG 
GGCACCTTCT TCAAGCAACG CATCCTCGAT CAGCTGCGCA TGATGCACTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assembly reconciliation.
Bioinformatics (Oxford, England)24(1)42-5(2008 Jan)
Zimin AV,Smith DR,Sutton G,Yorke JA

Evolution of genes and genomes on the Drosophila phylogeny.
Nature450(7167)203-18(2007 Nov)
,Clark AG,Eisen MB,Smith DR,Bergman CM,Oliver B,Markow TA,Kaufman TC,Kellis M,Gelbart W,Iyer VN,Pollard DA,Sackton TB,Larracuente AM,Singh ND,Abad JP,Abt DN,Adryan B,Aguade M,Akashi H,Anderson WW,Aquadro CF,Ardell DH,Arguello R,Artieri CG,Barbash DA,Barker D,Barsanti P,Batterham P,Batzoglou S,Begun D,Bhutkar A,Blanco E,Bosak SA,Bradley RK,Brand AD,Brent MR,Brooks AN,Brown RH,Butlin RK,Caggese C,Calvi BR,Bernardo de Carvalho A,Caspi A,Castrezana S,Celniker SE,Chang JL,Chapple C,Chatterji S,Chinwalla A,Civetta A,Clifton SW,Comeron JM,Costello JC,Coyne JA,Daub J,David RG,Delcher AL,Delehaunty K,Do CB,Ebling H,Edwards K,Eickbush T,Evans JD,Filipski A,Findeiss S,Freyhult E,Fulton L,Fulton R,Garcia AC,Gardiner A,Garfield DA,Garvin BE,Gibson G,Gilbert D,Gnerre S,Godfrey J,Good R,Gotea V,Gravely B,Greenberg AJ,Griffiths-Jones S,Gross S,Guigo R,Gustafson EA,Haerty W,Hahn MW,Halligan DL,Halpern AL,Halter GM,Han MV,Heger A,Hillier L,Hinrichs AS,Holmes I,Hoskins RA,Hubisz MJ,Hultmark D,Huntley MA,Jaffe DB,Jagadeeshan S,Jeck WR,Johnson J,Jones CD,Jordan WC,Karpen GH,Kataoka E,Keightley PD,Kheradpour P,Kirkness EF,Koerich LB,Kristiansen K,Kudrna D,Kulathinal RJ,Kumar S,Kwok R,Lander E,Langley CH,Lapoint R,Lazzaro BP,Lee SJ,Levesque L,Li R,Lin CF,Lin MF,Lindblad-Toh K,Llopart A,Long M,Low L,Lozovsky E,Lu J,Luo M,Machado CA,Makalowski W,Marzo M,Matsuda M,Matzkin L,McAllister B,McBride CS,McKernan B,McKernan K,Mendez-Lago M,Minx P,Mollenhauer MU,Montooth K,Mount SM,Mu X,Myers E,Negre B,Newfeld S,Nielsen R,Noor MA,O'Grady P,Pachter L,Papaceit M,Parisi MJ,Parisi M,Parts L,Pedersen JS,Pesole G,Phillippy AM,Ponting CP,Pop M,Porcelli D,Powell JR,Prohaska S,Pruitt K,Puig M,Quesneville H,Ram KR,Rand D,Rasmussen MD,Reed LK,Reenan R,Reily A,Remington KA,Rieger TT,Ritchie MG,Robin C,Rogers YH,Rohde C,Rozas J,Rubenfield MJ,Ruiz A,Russo S,Salzberg SL,Sanchez-Gracia A,Saranga DJ,Sato H,Schaeffer SW,Schatz MC,Schlenke T,Schwartz R,Segarra C,Singh RS,Sirot L,Sirota M,Sisneros NB,Smith CD,Smith TF,Spieth J,Stage DE,Stark A,Stephan W,Strausberg RL,Strempel S,Sturgill D,Sutton G,Sutton GG,Tao W,Teichmann S,Tobari YN,Tomimura Y,Tsolas JM,Valente VL,Venter E,Venter JC,Vicario S,Vieira FG,Vilella AJ,Villasante A,Walenz B,Wang J,Wasserman M,Watts T,Wilson D,Wilson RK,Wing RA,Wolfner MF,Wong A,Wong GK,Wu CI,Wu G,Yamamoto D,Yang HP,Yang SP,Yorke JA,Yoshida K,Zdobnov E,Zhang P,Zhang Y,Zimin AV,Baldwin J,Abdouelleil A,Abdulkadir J,Abebe A,Abera B,Abreu J,Acer SC,Aftuck L,Alexander A,An P,Anderson E,Anderson S,Arachi H,Azer M,Bachantsang P,Barry A,Bayul T,Berlin A,Bessette D,Bloom T,Blye J,Boguslavskiy L,Bonnet C,Boukhgalter B,Bourzgui I,Brown A,Cahill P,Channer S,Cheshatsang Y,Chuda L,Citroen M,Collymore A,Cooke P,Costello M,D'Aco K,Daza R,De Haan G,DeGray S,DeMaso C,Dhargay N,Dooley K,Dooley E,Doricent M,Dorje P,Dorjee K,Dupes A,Elong R,Falk J,Farina A,Faro S,Ferguson D,Fisher S,Foley CD,Franke A,Friedrich D,Gadbois L,Gearin G,Gearin CR,Giannoukos G,Goode T,Graham J,Grandbois E,Grewal S,Gyaltsen K,Hafez N,Hagos B,Hall J,Henson C,Hollinger A,Honan T,Huard MD,Hughes L,Hurhula B,Husby ME,Kamat A,Kanga B,Kashin S,Khazanovich D,Kisner P,Lance K,Lara M,Lee W,Lennon N,Letendre F,LeVine R,Lipovsky A,Liu X,Liu J,Liu S,Lokyitsang T,Lokyitsang Y,Lubonja R,Lui A,MacDonald P,Magnisalis V,Maru K,Matthews C,McCusker W,McDonough S,Mehta T,Meldrim J,Meneus L,Mihai O,Mihalev A,Mihova T,Mittelman R,Mlenga V,Montmayeur A,Mulrain L,Navidi A,Naylor J,Negash T,Nguyen T,Nguyen N,Nicol R,Norbu C,Norbu N,Novod N,O'Neill B,Osman S,Markiewicz E,Oyono OL,Patti C,Phunkhang P,Pierre F,Priest M,Raghuraman S,Rege F,Reyes R,Rise C,Rogov P,Ross K,Ryan E,Settipalli S,Shea T,Sherpa N,Shi L,Shih D,Sparrow T,Spaulding J,Stalker J,Stange-Thomann N,Stavropoulos S,Stone C,Strader C,Tesfaye S,Thomson T,Thoulutsang Y,Thoulutsang D,Topham K,Topping I,Tsamla T,Vassiliev H,Vo A,Wangchuk T,Wangdi T,Weiand M,Wilkinson J,Wilson A,Yadav S,Young G,Yu Q,Zembek L,Zhong D,Zimmer A,Zwirko Z,Jaffe DB,Alvarez P,Brockman W,Butler J,Chin C,Gnerre S,Grabherr M,Kleber M,Mauceli E,MacCallum I

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Dere\GG22325 cDNA ORF clone, Drosophila erecta

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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