TLN2A gene cDNA ORF clone, Danio rerio(Zebrafish)

mRNA	Protein	Name
XM_009303223.2	XP_009301498.1	talin-2 isoform X1
XM_005168882.4	XP_005168939.1	talin-2 isoform X2
XM_017356865.2	XP_017212354.1	talin-2 isoform X1
XM_009303223.3	XP_009301498.1	talin-2 isoform X1
XM_005168882.3	XP_005168939.1	talin-2 isoform X2
NM_201193.2	NP_957487.2	talin-2
XM_009303222.2	XP_009301497.1	talin-2 isoform X1
XM_009303222.3	XP_009301497.1	talin-2 isoform X1
XM_009303224.2	XP_009301499.1	talin-2 isoform X1
XM_017356865.1	XP_017212354.1	talin-2 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31298	NM_201193.2 Latest version!	Danio rerio talin 2a (tln2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa13079	XM_005168882.4 Latest version!	Danio rerio talin 2a (tln2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa42442	XM_017356865.2 Latest version!	Danio rerio talin 2a (tln2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa42442	XM_009303222.3 Latest version!	Danio rerio talin 2a (tln2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa42442	XM_009303224.2 Latest version!	Danio rerio talin 2a (tln2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa42442	XM_009303223.3 Latest version!	Danio rerio talin 2a (tln2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
View Old Accession Versions >>
ODa42442	XM_009303223.2	Danio rerio talin 2a (tln2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa13079	XM_005168882.3	Danio rerio talin 2a (tln2a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa42442	XM_009303222.2	Danio rerio talin 2a (tln2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
ODa42442	XM_017356865.1	Danio rerio talin 2a (tln2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

TLN2A ( NM_201193.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_957487.2 Danio rerio talin 2a (tln2a), mRNA.

CloneID	ODa31298
Clone ID Related Accession (Same CDS sequence)	NM_201193.2
Accession Version	NM_201193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1562860800000
Organism	Danio rerio(zebrafish)
Product	talin-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX914219.10 and CU856238.8. On or before Jun 19, 2011 this sequence version replaced XM_679764.4, NM_201193.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA4476740, SAMEA4476749 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG GCACTGAGTG GGTTGACCCT 
GAGGATCCTA CAGTCATCGC AGAAACGGAG TTACTGGGAG CTGCGGCCTC AATCGAAGCA 
GCAGCCAAAA AACTTGAGCA GCTTAAACCG AGAGCCAAGC CAAAGCAAGC TGACGAGAGT 
TTAGACTTTG AAGAGCAGAT TCTTGAAGCA GCCAAGTCAA TCGCTGCAGC GACCAGCGCT 
CTCGTCAAAT CAGCTTCAGC AGCTCAAAGA GAACTCGTAG CCCAGGGCAA GGTGGGAGCC 
ATTCCTGCTA ATGCTGTGGA TGATGGTCAG TGGTCCCAAG GACTGATATC TGCTGCTCGA 
ATGGTTGCTG CAGCCACTAG TAACCTCTGT GAGGCTGCAA ACGCGTCTGT CCAAGGCCAC 
GCCAGTGAAG AAAAGCTCAT CTCTTCTGCA AAACAAGTGG CTGCTTCCAC TGCACAGCTG 
CTGGTGGCAT GCAAAGTTAA AGCAGACCAA GATTCAGAGG CTATGAGGAG GCTACAGGCT 
GCAGGCAATG CTGTGAAACG TGCCTCTGAC AATCTGGTTC GAGCAGCACA GAAAGCTGCT 
TTCAACAAGG CTGATGACGA CAATGTGGTG GTCAAGACCA GGTTTGTTGG AGGAATTGCA 
CAGATTATTG CAGCTCAGGA GGAGATGTTA AGGAAGGAGA GGGAGCTGGA AGAAGCCCGT 
AAGAAGCTGG CACAGATCAG ACAACAGCAG TACAAGTTCC TGCCAAGTGA ACTCCGAGAG 
GATGACGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957487.2
CDS	170..7801
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	NM_201193.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 2a (tln2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201193.2

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG GCACTGAGTG GGTTGACCCT 
GAGGATCCTA CAGTCATCGC AGAAACGGAG TTACTGGGAG CTGCGGCCTC AATCGAAGCA 
GCAGCCAAAA AACTTGAGCA GCTTAAACCG AGAGCCAAGC CAAAGCAAGC TGACGAGAGT 
TTAGACTTTG AAGAGCAGAT TCTTGAAGCA GCCAAGTCAA TCGCTGCAGC GACCAGCGCT 
CTCGTCAAAT CAGCTTCAGC AGCTCAAAGA GAACTCGTAG CCCAGGGCAA GGTGGGAGCC 
ATTCCTGCTA ATGCTGTGGA TGATGGTCAG TGGTCCCAAG GACTGATATC TGCTGCTCGA 
ATGGTTGCTG CAGCCACTAG TAACCTCTGT GAGGCTGCAA ACGCGTCTGT CCAAGGCCAC 
GCCAGTGAAG AAAAGCTCAT CTCTTCTGCA AAACAAGTGG CTGCTTCCAC TGCACAGCTG 
CTGGTGGCAT GCAAAGTTAA AGCAGACCAA GATTCAGAGG CTATGAGGAG GCTACAGGCT 
GCAGGCAATG CTGTGAAACG TGCCTCTGAC AATCTGGTTC GAGCAGCACA GAAAGCTGCT 
TTCAACAAGG CTGATGACGA CAATGTGGTG GTCAAGACCA GGTTTGTTGG AGGAATTGCA 
CAGATTATTG CAGCTCAGGA GGAGATGTTA AGGAAGGAGA GGGAGCTGGA AGAAGCCCGT 
AAGAAGCTGG CACAGATCAG ACAACAGCAG TACAAGTTCC TGCCAAGTGA ACTCCGAGAG 
GATGACGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_005168882.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168939.1 Danio rerio talin 2a (tln2a), transcript variant X4, mRNA.

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CloneID	ODa13079
Clone ID Related Accession (Same CDS sequence)	XM_005168882.4 , XM_005168882.3
Accession Version	XM_005168882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	talin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168882.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGCAC TGAGTGGGTT 
GACCCTGAGG ATCCTACAGT CATCGCAGAA ACGGAGTTAC TGGGAGCTGC GGCCTCAATC 
GAAGCAGCAG CCAAAAAACT TGAGCAGCTT AAACCGAGAG CCAAGCCAAA GCAAGCTGAC 
GAGAGTTTAG ACTTTGAAGA GCAGATTCTT GAAGCAGCCA AGTCAATCGC TGCAGCGACC 
AGCGCTCTCG TCAAATCAGC TTCAGCAGCT CAAAGAGAAC TCGTAGCCCA GGGCAAGGTG 
GGAGCCATTC CTGCTAATGC TGTGGATGAT GGTCAGTGGT CCCAAGGACT GATATCTGCT 
GCTCGAATGG TTGCTGCAGC CACTAGTAAC CTCTGTGAGG CTGCAAACGC GTCTGTCCAA 
GGCCACGCCA GTGAAGAAAA GCTCATCTCT TCTGCAAAAC AAGTGGCTGC TTCCACTGCA 
CAGCTGCTGG TGGCATGCAA AGTTAAAGCA GACCAAGATT CAGAGGCTAT GAGGAGGCTA 
CAGGCTGCAG GCAATGCTGT GAAACGTGCC TCTGACAATC TGGTTCGAGC AGCACAGAAA 
GCTGCTTTCA ACAAGGCTGA TGACGACAAT GTGGTGGTCA AGACCAGGTT TGTTGGAGGA 
ATTGCACAGA TTATTGCAGC TCAGGAGGAG ATGTTAAGGA AGGAGAGGGA GCTGGAAGAA 
GCCCGTAAGA AGCTGGCACA GATCAGACAA CAGCAGTACA AGTTCCTGCC AAGTGAACTC 
CGAGAGGATG ACGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168939.1
CDS	192..7829
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_005168882.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168882.4

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGCAC TGAGTGGGTT 
GACCCTGAGG ATCCTACAGT CATCGCAGAA ACGGAGTTAC TGGGAGCTGC GGCCTCAATC 
GAAGCAGCAG CCAAAAAACT TGAGCAGCTT AAACCGAGAG CCAAGCCAAA GCAAGCTGAC 
GAGAGTTTAG ACTTTGAAGA GCAGATTCTT GAAGCAGCCA AGTCAATCGC TGCAGCGACC 
AGCGCTCTCG TCAAATCAGC TTCAGCAGCT CAAAGAGAAC TCGTAGCCCA GGGCAAGGTG 
GGAGCCATTC CTGCTAATGC TGTGGATGAT GGTCAGTGGT CCCAAGGACT GATATCTGCT 
GCTCGAATGG TTGCTGCAGC CACTAGTAAC CTCTGTGAGG CTGCAAACGC GTCTGTCCAA 
GGCCACGCCA GTGAAGAAAA GCTCATCTCT TCTGCAAAAC AAGTGGCTGC TTCCACTGCA 
CAGCTGCTGG TGGCATGCAA AGTTAAAGCA GACCAAGATT CAGAGGCTAT GAGGAGGCTA 
CAGGCTGCAG GCAATGCTGT GAAACGTGCC TCTGACAATC TGGTTCGAGC AGCACAGAAA 
GCTGCTTTCA ACAAGGCTGA TGACGACAAT GTGGTGGTCA AGACCAGGTT TGTTGGAGGA 
ATTGCACAGA TTATTGCAGC TCAGGAGGAG ATGTTAAGGA AGGAGAGGGA GCTGGAAGAA 
GCCCGTAAGA AGCTGGCACA GATCAGACAA CAGCAGTACA AGTTCCTGCC AAGTGAACTC 
CGAGAGGATG ACGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_005168882.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168939.1 Danio rerio talin 2a (tln2a), transcript variant X5, mRNA.

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CloneID	ODa13079
Clone ID Related Accession (Same CDS sequence)	XM_005168882.4 , XM_005168882.3
Accession Version	XM_005168882.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	talin-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168882.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGCAC TGAGTGGGTT 
GACCCTGAGG ATCCTACAGT CATCGCAGAA ACGGAGTTAC TGGGAGCTGC GGCCTCAATC 
GAAGCAGCAG CCAAAAAACT TGAGCAGCTT AAACCGAGAG CCAAGCCAAA GCAAGCTGAC 
GAGAGTTTAG ACTTTGAAGA GCAGATTCTT GAAGCAGCCA AGTCAATCGC TGCAGCGACC 
AGCGCTCTCG TCAAATCAGC TTCAGCAGCT CAAAGAGAAC TCGTAGCCCA GGGCAAGGTG 
GGAGCCATTC CTGCTAATGC TGTGGATGAT GGTCAGTGGT CCCAAGGACT GATATCTGCT 
GCTCGAATGG TTGCTGCAGC CACTAGTAAC CTCTGTGAGG CTGCAAACGC GTCTGTCCAA 
GGCCACGCCA GTGAAGAAAA GCTCATCTCT TCTGCAAAAC AAGTGGCTGC TTCCACTGCA 
CAGCTGCTGG TGGCATGCAA AGTTAAAGCA GACCAAGATT CAGAGGCTAT GAGGAGGCTA 
CAGGCTGCAG GCAATGCTGT GAAACGTGCC TCTGACAATC TGGTTCGAGC AGCACAGAAA 
GCTGCTTTCA ACAAGGCTGA TGACGACAAT GTGGTGGTCA AGACCAGGTT TGTTGGAGGA 
ATTGCACAGA TTATTGCAGC TCAGGAGGAG ATGTTAAGGA AGGAGAGGGA GCTGGAAGAA 
GCCCGTAAGA AGCTGGCACA GATCAGACAA CAGCAGTACA AGTTCCTGCC AAGTGAACTC 
CGAGAGGATG ACGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168939.1
CDS	169..7806
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_005168882.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168882.3

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGCAC TGAGTGGGTT 
GACCCTGAGG ATCCTACAGT CATCGCAGAA ACGGAGTTAC TGGGAGCTGC GGCCTCAATC 
GAAGCAGCAG CCAAAAAACT TGAGCAGCTT AAACCGAGAG CCAAGCCAAA GCAAGCTGAC 
GAGAGTTTAG ACTTTGAAGA GCAGATTCTT GAAGCAGCCA AGTCAATCGC TGCAGCGACC 
AGCGCTCTCG TCAAATCAGC TTCAGCAGCT CAAAGAGAAC TCGTAGCCCA GGGCAAGGTG 
GGAGCCATTC CTGCTAATGC TGTGGATGAT GGTCAGTGGT CCCAAGGACT GATATCTGCT 
GCTCGAATGG TTGCTGCAGC CACTAGTAAC CTCTGTGAGG CTGCAAACGC GTCTGTCCAA 
GGCCACGCCA GTGAAGAAAA GCTCATCTCT TCTGCAAAAC AAGTGGCTGC TTCCACTGCA 
CAGCTGCTGG TGGCATGCAA AGTTAAAGCA GACCAAGATT CAGAGGCTAT GAGGAGGCTA 
CAGGCTGCAG GCAATGCTGT GAAACGTGCC TCTGACAATC TGGTTCGAGC AGCACAGAAA 
GCTGCTTTCA ACAAGGCTGA TGACGACAAT GTGGTGGTCA AGACCAGGTT TGTTGGAGGA 
ATTGCACAGA TTATTGCAGC TCAGGAGGAG ATGTTAAGGA AGGAGAGGGA GCTGGAAGAA 
GCCCGTAAGA AGCTGGCACA GATCAGACAA CAGCAGTACA AGTTCCTGCC AAGTGAACTC 
CGAGAGGATG ACGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_009303223.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301498.1 Danio rerio talin 2a (tln2a), transcript variant X2, mRNA.

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CloneID	ODa42442
Clone ID Related Accession (Same CDS sequence)	XM_009303223.2 , XM_017356865.2 , XM_009303223.3 , XM_009303222.2 , XM_009303222.3 , XM_009303224.2 , XM_017356865.1
Accession Version	XM_009303223.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	talin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303223.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301498.1
CDS	294..7940
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGVYSTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_009303223.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303223.2

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_017356865.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212354.1 Danio rerio talin 2a (tln2a), transcript variant X3, mRNA.

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CloneID	ODa42442
Clone ID Related Accession (Same CDS sequence)	XM_009303223.2 , XM_017356865.2 , XM_009303223.3 , XM_009303222.2 , XM_009303222.3 , XM_009303224.2 , XM_017356865.1
Accession Version	XM_017356865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	talin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212354.1
CDS	318..7964
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGVYSTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_017356865.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356865.2

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_009303223.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301498.1 Danio rerio talin 2a (tln2a), transcript variant X2, mRNA.

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CloneID	ODa42442
Clone ID Related Accession (Same CDS sequence)	XM_009303223.2 , XM_017356865.2 , XM_009303223.3 , XM_009303222.2 , XM_009303222.3 , XM_009303224.2 , XM_017356865.1
Accession Version	XM_009303223.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	talin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303223.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301498.1
CDS	315..7961
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGVYSTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_009303223.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303223.3

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_009303222.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301497.1 Danio rerio talin 2a (tln2a), transcript variant X1, mRNA.

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CloneID	ODa42442
Clone ID Related Accession (Same CDS sequence)	XM_009303223.2 , XM_017356865.2 , XM_009303223.3 , XM_009303222.2 , XM_009303222.3 , XM_009303224.2 , XM_017356865.1
Accession Version	XM_009303222.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	talin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301497.1
CDS	169..7815
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGVYSTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_009303222.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303222.2

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_009303222.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301497.1 Danio rerio talin 2a (tln2a), transcript variant X1, mRNA.

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CloneID	ODa42442
Clone ID Related Accession (Same CDS sequence)	XM_009303223.2 , XM_017356865.2 , XM_009303223.3 , XM_009303222.2 , XM_009303222.3 , XM_009303224.2 , XM_017356865.1
Accession Version	XM_009303222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497369600000
Organism	Danio rerio(zebrafish)
Product	talin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301497.1
CDS	192..7838
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGVYSTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_009303222.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303222.3

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_009303224.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009301499.1 Danio rerio talin 2a (tln2a), transcript variant X3, mRNA.

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CloneID	ODa42442
Clone ID Related Accession (Same CDS sequence)	XM_009303223.2 , XM_017356865.2 , XM_009303223.3 , XM_009303222.2 , XM_009303222.3 , XM_009303224.2 , XM_017356865.1
Accession Version	XM_009303224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	talin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303224.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301499.1
CDS	372..8018
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGVYSTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_009303224.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303224.2

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TLN2A ( XM_017356865.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212354.1 Danio rerio talin 2a (tln2a), transcript variant X4, mRNA.

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CloneID	ODa42442
Clone ID Related Accession (Same CDS sequence)	XM_009303223.2 , XM_017356865.2 , XM_009303223.3 , XM_009303222.2 , XM_009303222.3 , XM_009303224.2 , XM_017356865.1
Accession Version	XM_017356865.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466611200000
Organism	Danio rerio(Zebrafish)
Product	talin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212354.1
CDS	300..7946
Translation	MVALSLKICVRQCNVVKTMQFEPSTAVYDACRVIRERVPEAQTGQASEYGLFLSDEDPRKGIWLEGGRTLDYYMLRNGDILEYKKKQRPQKVKMLDGAVKTIMVDDSKTVGELLVTICSRIGITNYEEYSLIQETVEEKKDEGTGTLKKDRTLLREERKMEKLKAKLHTDDDLNWLDHGRTFREQGVEESETLLLRRKFFYSDQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFGGIQTQIQFGPHIEHKHKTGFLDLKEFLPKEYIKQRGAEKKIFQEHKGCGEMTEIEAKVKYVKLARSLRTYGVSFFLVKEKMKSKNKLVPRLLGVTKESVMRVDEKTKDVVQEWPLTTVKRWAASPKSFTLDFGEYQESYYSVQTTEGEQISQLIAGYIDIILKKKQSKDRFGLEGDEEATMLEESVSPKKSTILQQQFNRVGRVEHGSVALPGILRSGSVGAETLNMGTMPAPQQQITTGQMHLGHMPPLSAAQQALVGTINTSMQAVQQAQDDLEEVEELPPLGDDMASKMWIQNKMDESKHDIHSQVDAITAGTASVVNLTAGDPTDTDYTAVGCAITTISSNLTEMSKGVKLLAALMEDEVGSGHDLMRAARTLTGAVSDLLKAVEPTSGEPRQTVLTAAGSIGQASGDLLRQIGENETDERFQDVLMNLAKAVANAAAMMVLKAKNVAQVSEDSVMQNRVIAAATQCALSTSQLVACAKVVSPTISSPVCQEQLIEAGKLVDRSVEGCVKACLSATSDGELLKAVSAAAGIVTQALSDLLTHVRNYATRGEPIGRYDQATDTIMNVTENIFSSMGDAGEMVRQARVLAQATSDLVNAMRSDAEVEVDVNNSKKLLAAAKLLADATARMVEAAKGAAAYPENEDQQQKLREAAEGLRVATNAAAQNAIKKKLITRLENAAKQAAAAATQTIAASQNAAASNKNTAAHQQLVQSCRAVADHIPQLVQGVRGSQGQPEDGSAQLALIIASKNFLQPGSKMVSSAKSSVPTVTDQAAAMQLGQCAKNLATTLAELRTAAQTAHEACGPMEIDSALIAVQTLKNELQDAKMAASEGQLKPLPGESLEKCAQDLGGTSKAVGSSMAQLLTCAAQGNEHYTGVAASETAQALKTLAQAARGVAATTSDPAGAAAMLDSAREVMEGSAKLISEAKEALVAPGDAEIQQRLAQVAKAVSHSLNACVNCLPGQKDVDMALKSIGEASKKLLVETLPPCSKSFQEAQSDLNQTAADLNQSAGDVVHASRGTTTQLADASGKFSDNFDEFLDAGIEMAGHTPGKDDQLQVIGNLKNISMASSKLLLAAKSLSVDPGAGNAKNLLAAAARSVTDSINQLITMCTQNAPGQRECDNALRELEAVRGLLDNPNEPVNDLSYFDCIESVMENSKVLGESMAGISHNCKTGDVPAFGDCVGGASKALCGLTEAAGQASYLVGVSDPSSQAGHPGLVDPIQFARANQAIQMACQNLVDPDSSPSQVLSAATIVAKHTSALCNACRLASSKTANPVAKRHFVQSAKEVANSTANLVKTIKALDGDFSEENREKCRIATAPLIEAVDNLTIFASNPEFASVPAQISREGSAAQEPIIQSARSMLDSSTHLLKTARSLVMNPKDPPTWSLLAGHSRTVSDSIKSLITAIRDKAPGQKECDSAIDNINKCIRDIEQASLAAVSQNLPRRDEVSSEALQEQLTSSVQEVGHLIEPITTAAKGEAAQLGHKVSQLVSYFTPLVSASMGIASKTLDHQQQMNLLDQTKTLAESALQMLYAAKEGGGNPKASHTHDAIAEAAQLMREAVDDLLLTLNEAASEVGMVSGMVDSIADSMGKLGEGTPPEPEGSFVEYQTTMVRYSKAIAVSAQEMITKSVSSPEDLCSLASQVTADYSQLAVQGRLASATAEPEEIGFQIKTSVQELGHGCIVLVQKAGALQISPTDSFTKRELIECARAVTEKVSMVLSALQAGNKGTQACITAASAVSGIIADLDTTIMFASAGTLNAENDSDSFADHRENILKTAKALVEDTKNLVSGAASSQEKLAQAAQSSAKTITQLTDVVKLGAASIGPDDPETQVVLINAVKDVAKALAELISATKCAAGKAADDPSMFQLKSAAKVMVTNVTSLLKTVKAVEDEASRGTRALEATIECIRQELTMFQSKETPEKSSTPEEFIRMTKGITTATAKAVAAGNSARQEDVISTANLSRKVIADMLTSCKQAAFNEEVSVEVRSRALLYGTECTNGYIDLLEHVLQVLQKPTGDQKQKLAMFSKKVAAAVTELIQTAEAMKDEGVYSTEWVDPEDPTVIAETELLGAAASIEAAAKKLEQLKPRAKPKQADESLDFEEQILEAAKSIAAATSALVKSASAAQRELVAQGKVGAIPANAVDDGQWSQGLISAARMVAAATSNLCEAANASVQGHASEEKLISSAKQVAASTAQLLVACKVKADQDSEAMRRLQAAGNAVKRASDNLVRAAQKAAFNKADDDNVVVKTRFVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELREDDD

Target ORF information:

RefSeq Version	XM_017356865.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio talin 2a (tln2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356865.1

ORF Insert Sequence:

ATGGTGGCGC TGTCTCTGAA GATCTGTGTG AGGCAGTGTA ATGTGGTGAA GACCATGCAG 
TTTGAGCCCT CAACGGCTGT ATATGACGCA TGCCGGGTCA TCAGAGAGAG GGTTCCAGAA 
GCCCAGACAG GACAAGCTTC AGAATATGGG CTGTTTCTGT CAGACGAAGA CCCTAGGAAA 
GGCATCTGGC TAGAAGGAGG CCGGACGCTG GATTATTACA TGCTGAGAAA TGGAGACATA 
TTGGAGTATA AGAAAAAACA GAGGCCTCAG AAGGTAAAAA TGCTGGACGG AGCTGTTAAG 
ACCATCATGG TGGACGATTC TAAAACTGTT GGCGAGCTGC TGGTCACCAT CTGCAGCAGA 
ATAGGAATCA CCAACTATGA AGAGTACTCT CTCATTCAGG AAACTGTGGA GGAGAAGAAA 
GATGAAGGGA CAGGCACACT GAAGAAAGAC AGAACTCTGC TAAGAGAGGA GAGGAAGATG 
GAGAAGCTCA AAGCCAAACT GCACACAGAT GATGACTTGA ATTGGTTGGA TCATGGCCGT 
ACATTTAGGG AACAGGGAGT CGAGGAAAGT GAAACATTAC TTTTGAGACG AAAGTTCTTC 
TACTCCGATC AAAACGTGGA CTCGCGAGAT CCTGTGCAGC TCAATCTGCT CTACGTGCAG 
GCACGTGATG ACATTCTGAA CGGTTCTCAT CCGGTGTCGT TTGACAAGGC TTGTGAGTTT 
GGGGGAATTC AAACGCAGAT CCAGTTCGGT CCTCATATTG AACATAAACA CAAGACTGGA 
TTTCTGGACT TGAAGGAGTT TCTGCCTAAG GAATATATCA AACAGAGAGG AGCGGAGAAG 
AAGATCTTTC AGGAACACAA GGGTTGTGGT GAAATGACTG AGATTGAAGC CAAAGTGAAG 
TATGTGAAGC TTGCAAGATC CCTCAGAACA TATGGAGTGT CTTTCTTCTT GGTGAAGGAA 
AAAATGAAAA GTAAAAATAA GCTGGTGCCA AGGCTACTGG GTGTAACCAA AGAGTCAGTG 
ATGCGAGTGG ATGAGAAGAC CAAAGACGTG GTTCAGGAGT GGCCTCTTAC TACAGTGAAG 
AGATGGGCCG CCTCACCCAA GAGCTTTACA CTGGATTTCG GGGAGTATCA GGAAAGCTAC 
TACTCTGTTC AAACCACTGA GGGGGAGCAG ATTTCTCAGC TTATAGCTGG TTACATCGAT 
ATCATTCTCA AGAAGAAACA AAGTAAGGAC CGGTTTGGTT TAGAGGGAGA TGAGGAGGCC 
ACCATGTTAG AGGAATCCGT TTCTCCAAAA AAATCAACTA TATTGCAGCA GCAGTTTAAC 
CGTGTGGGAC GGGTCGAGCA TGGCTCTGTG GCACTGCCTG GCATCTTGAG GTCTGGTTCT 
GTTGGGGCAG AAACGCTGAA CATGGGTACA ATGCCTGCTC CACAACAACA GATCACAACT 
GGACAGATGC ATCTTGGACA CATGCCACCA CTGAGTGCAG CCCAGCAGGC TCTCGTGGGA 
ACCATTAACA CCAGTATGCA GGCGGTGCAG CAGGCGCAGG ACGACCTGGA GGAGGTGGAG 
GAGCTGCCAC CACTTGGGGA TGACATGGCT TCTAAGATGT GGATTCAGAA CAAAATGGAC 
GAGTCCAAGC ATGACATTCA CTCTCAGGTG GATGCCATCA CAGCTGGAAC TGCCTCTGTG 
GTCAATCTGA CTGCCGGTGA CCCAACAGAC ACGGATTACA CTGCAGTGGG CTGTGCCATC 
ACCACCATTT CCTCAAATCT GACAGAGATG TCGAAGGGAG TGAAGCTGCT GGCGGCTCTG 
ATGGAGGATG AGGTTGGCAG TGGGCATGAT CTGATGAGAG CTGCCAGGAC CCTAACCGGA 
GCCGTTTCTG ACCTGCTCAA AGCTGTCGAG CCCACGTCAG GAGAGCCTCG ACAGACTGTT 
CTGACTGCAG CTGGCAGCAT CGGTCAGGCC AGTGGAGATC TGCTCAGACA GATTGGAGAG 
AATGAAACCG ATGAGCGCTT CCAGGATGTG CTGATGAACT TGGCAAAAGC TGTGGCCAAT 
GCAGCAGCTA TGATGGTTCT AAAGGCCAAG AACGTGGCGC AGGTTTCTGA AGACAGCGTG 
ATGCAGAACA GGGTTATCGC TGCTGCCACC CAGTGTGCCC TTTCCACTTC ACAACTAGTG 
GCCTGTGCCA AGGTTGTGAG CCCCACGATC AGCTCTCCGG TATGTCAGGA GCAGCTGATT 
GAAGCAGGGA AACTGGTGGA TCGTTCGGTT GAAGGTTGTG TGAAGGCCTG TCTGTCGGCC 
ACCAGCGATG GAGAGCTCCT TAAGGCTGTC AGCGCAGCAG CCGGTATTGT CACACAGGCT 
CTAAGTGACC TCCTCACACA CGTCAGGAAC TACGCTACCC GCGGAGAGCC CATTGGACGC 
TACGACCAGG CCACAGACAC CATCATGAAC GTCACAGAGA ACATCTTCAG CTCTATGGGG 
GATGCTGGTG AGATGGTCCG GCAGGCGCGG GTTCTTGCCC AGGCGACCTC TGACCTTGTG 
AACGCCATGA GATCAGATGC TGAGGTAGAG GTGGATGTCA ACAACTCCAA GAAGCTTCTT 
GCGGCTGCCA AACTGCTGGC AGATGCCACT GCTCGAATGG TTGAGGCTGC AAAGGGAGCC 
GCTGCATACC CTGAAAACGA AGACCAGCAG CAGAAGTTGC GTGAGGCTGC AGAGGGTCTG 
CGTGTGGCCA CCAATGCTGC GGCACAGAAC GCCATTAAGA AGAAACTCAT AACAAGACTG 
GAGAATGCTG CTAAACAGGC CGCTGCTGCT GCCACTCAAA CCATTGCGGC GTCCCAGAAC 
GCAGCAGCTT CTAACAAAAA CACAGCAGCC CACCAACAAC TGGTTCAGAG CTGCAGGGCT 
GTAGCTGACC ATATCCCTCA GCTGGTGCAG GGAGTGCGTG GCAGTCAGGG GCAGCCAGAG 
GATGGAAGTG CACAGCTCGC TCTCATCATT GCCAGCAAGA ACTTCCTTCA GCCTGGAAGT 
AAGATGGTGT CTTCAGCAAA ATCCTCGGTG CCAACTGTGA CTGATCAGGC TGCAGCCATG 
CAGCTGGGGC AGTGTGCGAA GAATCTGGCC ACCACCCTGG CTGAACTCCG CACCGCTGCA 
CAGACGGCTC ATGAGGCTTG TGGCCCGATG GAGATCGACT CTGCCCTCAT CGCAGTTCAG 
ACACTTAAGA ATGAACTCCA GGATGCTAAA ATGGCTGCTT CTGAGGGACA GTTGAAACCT 
TTACCTGGAG AGTCCTTGGA AAAGTGCGCT CAGGATCTGG GTGGTACATC TAAGGCTGTT 
GGTTCCTCAA TGGCTCAACT GCTTACGTGT GCAGCACAGG GAAATGAGCA TTACACTGGT 
GTAGCCGCTA GTGAAACAGC ACAAGCACTG AAGACATTAG CTCAGGCCGC AAGGGGTGTT 
GCTGCCACCA CATCAGATCC CGCTGGAGCA GCTGCCATGT TAGATTCTGC TCGTGAGGTT 
ATGGAAGGAT CCGCTAAACT GATTTCTGAA GCCAAGGAGG CGCTGGTTGC TCCTGGGGAT 
GCAGAAATAC AACAGAGACT TGCACAGGTT GCAAAAGCTG TGTCTCACTC GCTGAACGCA 
TGTGTGAACT GCTTACCTGG ACAAAAGGAT GTCGATATGG CTCTAAAAAG CATCGGAGAG 
GCCAGCAAGA AATTGCTTGT TGAGACGCTC CCTCCGTGCA GTAAGAGCTT CCAGGAGGCT 
CAGAGTGACC TGAACCAGAC CGCAGCCGAT CTGAACCAGT CTGCAGGAGA TGTGGTCCAC 
GCCTCTCGAG GAACCACTAC TCAACTCGCA GACGCTTCTG GAAAGTTCAG TGACAACTTT 
GATGAGTTTC TGGATGCTGG TATTGAGATG GCTGGCCATA CTCCGGGTAA GGATGATCAG 
TTGCAGGTAA TTGGAAATTT GAAGAACATC TCGATGGCCT CTAGTAAGCT TCTGCTGGCA 
GCCAAGTCTT TATCTGTAGA CCCTGGAGCT GGCAACGCAA AGAACCTGCT CGCCGCCGCT 
GCTAGATCTG TGACTGACAG CATCAATCAG CTGATTACGA TGTGTACCCA GAATGCCCCG 
GGACAGAGGG AATGTGACAA TGCTTTAAGA GAGCTTGAGG CTGTTAGAGG ACTGCTGGAT 
AACCCAAATG AGCCGGTCAA TGATCTCTCC TACTTTGACT GCATTGAAAG TGTCATGGAG 
AACTCTAAGG TACTTGGAGA GTCTATGGCC GGGATTTCTC ACAACTGTAA AACAGGAGAC 
GTTCCAGCAT TTGGAGACTG TGTTGGTGGA GCGTCTAAAG CTCTGTGTGG ACTAACTGAA 
GCAGCTGGAC AGGCTTCTTA CCTAGTAGGT GTGTCTGATC CCAGCAGCCA AGCTGGACAT 
CCAGGCTTAG TGGATCCTAT CCAGTTTGCC CGTGCTAATC AGGCAATCCA GATGGCTTGC 
CAAAATCTTG TTGACCCAGA TAGTAGTCCA TCACAGGTGC TCTCAGCCGC CACTATTGTT 
GCTAAGCACA CCTCAGCACT TTGCAACGCT TGTCGACTTG CTTCCTCGAA GACTGCCAAC 
CCTGTGGCAA AACGACATTT CGTGCAGTCC GCCAAAGAAG TGGCAAACAG CACTGCCAAC 
CTGGTCAAAA CAATCAAGGC ATTGGATGGT GATTTTTCAG AGGAAAACCG TGAGAAATGC 
CGCATTGCAA CAGCTCCGCT CATTGAAGCT GTTGACAATT TGACAATATT TGCTTCTAAT 
CCAGAGTTTG CTAGTGTCCC AGCGCAGATA AGTAGAGAGG GTTCTGCTGC ACAGGAACCA 
ATCATCCAAT CAGCACGCTC CATGCTTGAC AGCTCCACCC ACCTTTTGAA GACAGCACGG 
TCATTGGTTA TGAACCCGAA AGATCCACCC ACATGGTCAT TACTGGCAGG TCATTCACGC 
ACTGTTTCCG ACTCCATTAA GAGCCTCATC ACAGCTATCA GGGATAAAGC CCCTGGCCAG 
AAAGAGTGTG ACTCAGCCAT TGATAATATC AATAAATGCA TCCGAGATAT TGAGCAGGCC 
TCTCTTGCAG CAGTCAGCCA GAATCTACCT CGTAGAGATG AAGTTTCATC TGAGGCTTTG 
CAGGAGCAGT TGACGTCATC TGTTCAGGAG GTCGGTCACC TGATTGAGCC AATTACCACA 
GCAGCGAAAG GAGAGGCAGC ACAGCTCGGA CATAAGGTCT CTCAGTTGGT CAGTTACTTC 
ACTCCTCTGG TCTCAGCCTC TATGGGCATA GCCTCTAAAA CTTTAGACCA CCAGCAGCAG 
ATGAACCTTC TGGATCAGAC CAAGACCCTC GCCGAGTCTG CTCTACAAAT GCTCTACGCT 
GCCAAGGAGG GTGGTGGAAA CCCAAAGGCG TCTCATACTC ATGATGCCAT AGCAGAAGCT 
GCGCAGTTAA TGAGGGAAGC TGTTGATGAC CTCCTGCTGA CCCTGAATGA AGCTGCTAGT 
GAGGTTGGCA TGGTCAGTGG CATGGTGGAC TCCATTGCTG ATTCCATGGG CAAGCTGGGT 
GAAGGCACCC CACCAGAGCC AGAAGGTTCA TTTGTGGAGT ATCAGACTAC CATGGTGAGA 
TACTCCAAAG CTATTGCTGT CAGTGCCCAA GAGATGATCA CGAAATCAGT CAGTTCTCCA 
GAGGATCTGT GCAGTTTGGC GTCTCAGGTC ACTGCAGACT ACAGTCAGCT TGCAGTTCAG 
GGTCGTCTGG CCTCTGCTAC TGCTGAACCA GAGGAGATCG GGTTCCAGAT CAAGACAAGT 
GTTCAGGAAC TGGGCCACGG CTGCATAGTG CTAGTTCAAA AGGCTGGAGC GCTGCAGATC 
AGTCCTACAG ACTCGTTCAC CAAGAGGGAA CTCATTGAGT GTGCACGAGC CGTCACTGAG 
AAGGTGTCTA TGGTGCTGTC TGCGTTGCAA GCTGGTAATA AAGGCACTCA AGCCTGTATC 
ACTGCGGCCA GTGCTGTATC CGGCATCATC GCTGACCTGG ACACCACCAT CATGTTTGCT 
TCAGCTGGCA CGCTCAACGC TGAGAATGAT TCAGACTCCT TTGCCGATCA CAGAGAAAAT 
ATTCTGAAGA CCGCCAAAGC TCTGGTTGAG GACACAAAGA ACCTTGTTTC AGGTGCTGCG 
TCGAGTCAAG AAAAGCTGGC TCAGGCCGCC CAGTCCTCTG CCAAGACCAT CACGCAGCTT 
ACAGATGTGG TGAAACTGGG AGCTGCCAGT ATAGGTCCCG ATGACCCAGA AACACAGGTG 
GTGTTGATCA ATGCAGTGAA GGACGTCGCT AAAGCTTTAG CTGAGCTCAT TAGCGCCACC 
AAGTGTGCAG CAGGAAAAGC AGCCGACGAC CCGTCCATGT TCCAGCTCAA GAGTGCAGCC 
AAGGTCATGG TAACTAATGT AACATCCTTA CTGAAAACCG TAAAAGCAGT GGAGGATGAG 
GCTTCAAGAG GAACCAGAGC GCTGGAGGCC ACTATTGAGT GCATCAGACA GGAGCTCACA 
ATGTTCCAGT CTAAAGAAAC CCCAGAGAAG TCCAGCACTC CAGAAGAGTT CATTCGCATG 
ACCAAGGGCA TCACCACGGC GACCGCCAAA GCTGTAGCCG CAGGAAACTC TGCCCGACAG 
GAGGACGTGA TCTCCACCGC CAACCTCAGT CGAAAGGTCA TAGCGGATAT GCTGACATCA 
TGCAAGCAAG CTGCATTTAA CGAGGAAGTG AGTGTGGAGG TCAGGAGCAG AGCTCTGTTA 
TACGGCACTG AATGTACCAA TGGATATATA GATCTGCTAG AACACGTACT GCAGGTCTTA 
CAAAAGCCGA CAGGTGATCA GAAGCAGAAG TTGGCGATGT TCTCTAAAAA GGTTGCAGCA 
GCCGTCACTG AACTGATTCA GACAGCCGAG GCCATGAAAG ACGAGGGTGT GTACAGCACT 
GAGTGGGTTG ACCCTGAGGA TCCTACAGTC ATCGCAGAAA CGGAGTTACT GGGAGCTGCG 
GCCTCAATCG AAGCAGCAGC CAAAAAACTT GAGCAGCTTA AACCGAGAGC CAAGCCAAAG 
CAAGCTGACG AGAGTTTAGA CTTTGAAGAG CAGATTCTTG AAGCAGCCAA GTCAATCGCT 
GCAGCGACCA GCGCTCTCGT CAAATCAGCT TCAGCAGCTC AAAGAGAACT CGTAGCCCAG 
GGCAAGGTGG GAGCCATTCC TGCTAATGCT GTGGATGATG GTCAGTGGTC CCAAGGACTG 
ATATCTGCTG CTCGAATGGT TGCTGCAGCC ACTAGTAACC TCTGTGAGGC TGCAAACGCG 
TCTGTCCAAG GCCACGCCAG TGAAGAAAAG CTCATCTCTT CTGCAAAACA AGTGGCTGCT 
TCCACTGCAC AGCTGCTGGT GGCATGCAAA GTTAAAGCAG ACCAAGATTC AGAGGCTATG 
AGGAGGCTAC AGGCTGCAGG CAATGCTGTG AAACGTGCCT CTGACAATCT GGTTCGAGCA 
GCACAGAAAG CTGCTTTCAA CAAGGCTGAT GACGACAATG TGGTGGTCAA GACCAGGTTT 
GTTGGAGGAA TTGCACAGAT TATTGCAGCT CAGGAGGAGA TGTTAAGGAA GGAGAGGGAG 
CTGGAAGAAG CCCGTAAGAA GCTGGCACAG ATCAGACAAC AGCAGTACAA GTTCCTGCCA 
AGTGAACTCC GAGAGGATGA CGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TLN2A
Entrez Gene ID	556842
Full Name	talin 2a
Synonyms	si:busm1-132m23.1,tln1l,tln2,zgc:152648,zgc:63613
General protein information	Preferred Names talin 2a Names talin-2 talin 2 talin1, like
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

Pan troglodytes (chimpanzee)	TLN2	XP_510461.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007474	XP_308398.3
Bos taurus (cattle)	TLN2	XP_002690890.1
Rattus norvegicus (Norway rat)	Tln2	XP_006243460.1
Caenorhabditis elegans (roundworm)	Y71G12B.11	NP_490886.2
Macaca mulatta (Rhesus monkey)	TLN2	XP_001101705.1
Canis lupus familiaris (dog)	TLN2	XP_005638536.1
Drosophila melanogaster (fruit fly)	rhea	NP_001246674.1
Mus musculus (house mouse)	Tln2	NP_001074711.2
Gallus gallus (chicken)	TLN2	XP_003641925.1
Homo sapiens (human)	TLN2	NP_055874.2
Danio rerio (zebrafish)	tln2a	NP_957487.2
Xenopus tropicalis (tropical clawed frog)	tln2	NP_001116884.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TLN2A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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