LOC100884166 cDNA ORF clone, Bombyx mori(domestic silkworm)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100884166
Entrez Gene ID	100884166
Full Name	chitin synthase A
Gene Type	protein-coding
Organism	Bombyx mori(domestic silkworm)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012694439.1	XP_012549893.1	chitin synthase A isoform X3
XM_012694437.1	XP_012549891.1	chitin synthase A isoform X1
XM_021351080.1	XP_021206755.1	chitin synthase A isoform X1
XM_012694438.1	XP_012549892.1	chitin synthase A isoform X2
NM_001258362.1	NP_001245291.1	chitin synthase A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

DNA methylation mediates BmDeaf1-regulated tissue- and stage-specific expression of BmCHSA-2b in the silkworm, Bombyx mori.
Xu G, Zhang J, Lyu H, Song Q, Feng Q, Xiang H, Zheng S
Epigenetics & chromatin11(1)32(2018 06)

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
Ou J, Deng HM, Zheng SC, Huang LH, Feng QL, Liu L
BMC genomics15820(2014 Sep)

Chitin synthase A: a novel epidermal development regulation gene in the larvae of Bombyx mori.
Zhuo W, Fang Y, Kong L, Li X, Sima Y, Xu S
Molecular biology reports41(7)4177-86(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC100884166 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100884166 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBg13343	NM_001258362.1 Latest version!	Bombyx mori chitin synthase A (LOC100884166), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBg14616	XM_012694437.1 Latest version!	Bombyx mori chitin synthase A (LOC100884166), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBg14617	XM_012694438.1 Latest version!	Bombyx mori chitin synthase A (LOC100884166), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBg14618	XM_012694439.1 Latest version!	Bombyx mori chitin synthase A (LOC100884166), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBg26982	XM_021351080.1 Latest version!	Bombyx mori chitin synthase A (LOC100884166), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100884166 ( NM_001258362.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> NP_001245291.1 Bombyx mori chitin synthase A (LOC100884166), mRNA.

Price & Availability↑

CloneID	OBg13343
Clone ID Related Accession (Same CDS sequence)	NM_001258362.1
Accession Version	NM_001258362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1538841600000
Organism	Bombyx mori(domestic silkworm)
Product	chitin synthase A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ320074.1. ##Evidence-Data-START## Transcript exon combination :: JQ320074.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN04216981 [ECO:0000350] ##Evidence-Data-END##

ATGTATAGTC AGCGCACAGT ACAAGAAACG AAAGGATGGG ACGTGTTCAG AGAATTTCCG 
CCGAAACAGG ACAGTGTGTC CATGGAGACT CAGAAATGTT TGGAATTCAC AGTGCGAATG 
TTAAAAGTGG TCGCTTACCT GGTCACCTTC ATCGTGGTCC TTGGATCAGG AGTTATCGCA 
AAGGGGACAA TTTTGTTCAT GACGTCACAA ATTAGGAAAG ACAAGCGATT AGAATTCTGC 
AATAAAAACT TAGGTCGAGA TAAGCAGTTT GTGGTGAGTT TACCAGACGA GGAGCGTGTT 
GCCTGGATGT GGGCTATCCT AGCTGCCTTT GCTATTCCGG AATTAGGAAC GATGATCAGG 
TCTATAAGAA TATGCTTCTT CAAAACATCA AAGAAGCCGA GTTTTGTACA ATTTTCTGTG 
GTCTTTATCG CTGAGTCCTT ACATACCATA GGAATGGGTT TACTATTCTT TAAAATCCTG 
CCTGAATTAG ATGTAGTCAA AGGAGCTATG ATAACGAACT GTCTCTGTAT AATTCCCGCG 
GTGCTCGGTC TGCTGTCAAG AAACTCTCGA GACTCGAAAC GATTTGTCAA AGTCATCGTC 
GATATGGCCG CTATAGTTGC GCAAGTGACC GGTTTTATAG TTTGGCCCCT GTTGGAAAAC 
AAAGCTGTTT TGTGGTTGAT CCCGATCTCT GCTTTATGTA TTTCGTTAGG TTGGTGGGAA 
AACTATGTGA CACGACAAAG TCCAATAGGA ATAATCAAAA GCCTGGGGAG ACTAAAAGAG 
GAATTAAATT TTACTCGCTA CTTTACGTAT CGATTCATCT CCGTGTGGAA GATTTTACTG 
TTTCTTTTCT GCAATCTATT CTTCATGTGG TTGGACGGAG ATGAACCGGC AATGTTTTTC 
CAACTTTTCA ATCCTGGATT TGGGCCACAT AGCATTGTCG TTGAAGAGGT TCAAATCCAA 
CTCGGGGGAA CGGTTATTCC AGATTTGGCA AACATAACCC TTACAGGAGA CTCCGTCGAA 
ATCGCGGCTG TACATAAGTC CGCCGTCTAC GTCATGATGA TCCAAATATT TGCAGCCTAC 
ATCTGCTACA TCTTTGGGAA ATTTGCTTGC AAAATTGTTA TTCAAGGGTT CAGTTACGCT 
TTTCCGATAA ACTTGGTCAT ACCTTTGGTG GTGAACTTTT TGATTGCTGC TTGTGGTATC 
AGAAATGGCG ACACCTGTTT CTTCCACGGA ACGATACCGG ACTACCTATT CTTCGAAAGC 
CCTCCAGTGT TTACGTTGAG CGATTTCATC TCTCGACAAA TGGCATGGGT TTGGTTGTTA 
TGGCTGCTAT CACAAACCTG GATCACTATT CATATTTGGA CCCCCAAAGC AGAACGTTTA 
GCTTCCACTG AGAAATTGTT TGTGTTGCCT ATGTACAATG GTCTACTCAT AGACCAAAGT 
ATGGCACTGA ATAGGAAAAG AGATGACCAT AAGGATGTCA AAACAGAAGA TCTTGCCGAA 
ATCGAAAAGG AAAAAGGTGA TGAATACTAT GAAACAATAT CAGTACATAC AGACAATACT 
GGATCTTCCC CAAAGACTGT GAAATCATCA GACCAGATCA CGAGGATTTA TGCTTGTGCT 
ACTATGTGGC ACGAGACCAA AGATGAGATG ATGGAGTTCT TGAAATCTAT TCTTCGTCTC 
GATGAAGATC AGTGTGCTCG CCGCGTCGCT CAGAAGTATC TTCGTGTAGT AGATCCTGAC 
TATTACGAAT TCGAAACTCA CATTTTCTTG GACGACGCTT TCGAAATTTC GGATCATAGT 
GACGACGAAT CTCAAGTAAA CCGGTTTGTC AAGCTTCTGG TAGATACCAT CGACGAAGCT 
GCTTCTAATG TACATCAAAC GAACATTCGT ATACGTCCAC CGAAGAGATA TCCTGCGCCA 
TACGGAGGTA GACTGACATG GGTGTTACCA GGAAAGACTA AAATGATCTG TCACTTGAAG 
GATAAGGCAA AGATTCGACA CAGGAAACGT TGGTCTCAGG TGATGTACAT GTACTACCTT 
CTCGGTCATC GTCTAATGGA ATTGGCTATT TCGGTTGATC GTAAAGAAGT TATGGCTGAG 
AATACTTACC TGCTTACACT CGATGGAGAT ATCGATTTCC AACCTCACGC TGTACGATTA 
CTCATTGATT TAATGAAGAA AAACAAGAAC CTCGGAGCTG CCTGCGGGCG TATTCACCCT 
GTTGGATCTG GACCTATGGT GTGGTATCAA TTATTCGAGT ATGCTATCGG ACATTGGCTG 
CAAAAGGCCA CCGAACACAT GATCGGCTGC GTGCTCTGTA GTCCCGGATG CTTCTCGCTG 
TTCAGAGGAA AGGCTCTGAT GGACGACAAC GTTATGAAGA AATATACTTT GAAATCTGAC 
GAAGCCCGTC ACTACGTACA ATACGATCAG GGAGAAGATC GATGGCTATG CACTCTGCTA 
CTTCAACGTG GCTATCGTGT AGAATATTCA GCTGCATCGG ACGCCTACAC CCATTGCCCT 
GAAGGTTTTA GCGAGTTCTA CAATCAACGA CGTAGATGGG TGCCTTCGAC CATTGCTAAC 
ATTATGGACT TGCTTGTGGA TTATAAACAT ACCATTAAAA TCAATGACAA TATTTCCTCA 
CCGTACATCG CATACCAGAT GATGTTGATG GGCGGTACGA TTCTGGGACC TGGAACTATA 
TTTCTTATGT TGGTGGGTGC TTTTGTGGCC GCTTTTAGAA TTGATAATTG GACATCCTTC 
GAATACAATC TATATCCTAT AATGTTGTTT ATGTTTGTTT GTTTCACAAT GAAGTCAGAA 
TATCAAGAAA TCGAGCAAGA AAAGAAAGAT GCCGAAGAGG CGAAAAAGAA GGCGAAACAA 
AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC AATTCTAACG AAGAGGAAGG CTCCATAGAA 
CTATCGTTTG CTGGTCTTTT TAAATGTTTA CTGTGTACTC ATCCTAAAGG AAACGAAGAA 
AAAGTACAGC TCATGCATAT CGCTTCAACA CTTGAGAAAT TGGAAAAGAA AATTGAAAAC 
GTCGAGAAGG CCGTGGATCC GCACGGTCTA TCCAGAAGTC GCAAACTATC GCTAGGCCCT 
CGTGGTAGCA CTAACGGAGA CCACCAGTTG GACGCTCTTA ATGAGGACCC CGAAGAAGAA 
AACGATTCCG ATTCCGACAC CGGCACTCTA TCCACTGAAC CAAGGGAACG ACGAGATGAT 
CTTATAAATC CATATTGGAT TGAGGATCCC GGCTTGAAAA AAGGCGAAGT AGATTTCTTG 
AGCCCGCCCG AGGTACAATT CTGGAAAGAC CTTATTGACA AGTATTTATA TCCTATTGAC 
GAAGACAAGG ACGAGAAGGC ACGTATTTCG AGAGACTTAA AAGAACTGAG AGATTCTTCT 
GTATTTTCAT TCTTTATGAT CAATGCTCTT TTTGTGCTCA TTGTATTTTT GCTACAATTG 
AACAAGGACA ACCTTCACAT TAAATGGCCC TTCGGAGTTA AGACTAACAT TACCTATGAT 
GAGGTCTCAC AAGAGGCTCG CATAGCAGGC GATCTACTAG AACTTCGCAA TAAATCAGTT 
TTCGCATTTG TTATGTTCAA TGCTTTATTT ATATTGATAG TATTTTTATT ACAACTCAAT 
AAAGATCAGC TTCACGTGGT CTGGCCTTTG GGAGTTAAGA CAAACATTAC GTACATGGAG 
GAAACAGGCC AGCTTCTCAT CTCGAAGGAA TACTTGCAAT TAGAGCCTAT CGGTCTGGTA 
TTTGTGTTCT TCTTTGCCTT GATTTTGGTC ATACAATTTT CGGCCATGTT GTTCCATCGA 
TTCGGAACTA TTTCGCATAT TCTATCATCG ACGGATCTCA ACTGGTTCTG TTCAAAGAAA 
TCGGAAGATT TGTCACAGGA TGCGCTTTTG GATAAGAATG CAATAGCAAT AGTGAAAGAC 
CTTCAGAAAT TAAACGGTCT GGACGATGAT TATGATAACG ATTCAGGATC TGGACCACAC 
AACGTCGGCC GAAGGAAGAC AATACACAAT TTAGAGAAAG CTAGACAGAA GAAACGTAAC 
ATAGGAACAC TAGATGTGGC TTTCAAGAAA CGATTCTTCA ATATGAATGC TAATGATGGT 
CCGGGTACCC CAGTTTTAAA TCGTAAAATG ACATTGAGAA GGGAGACATT GAAAGCTTTA 
GAGACCCGTC GTAACTCTGT AATGGCAGAA AGAAGGAAGT CGCAAATGCA GACACTTGGC 
GCTAATAATG AATACGGTGT TACTGGAATG CTCAATAATA ACGTTGGACC TCGTCATAGA 
GCATCGAATG CTAATATTTC AGTGAAGGAT GTATTCTCGG AGCCCAACGG AGGACAGATC 
AACAGGGCCT ACGAAGCCTC ACTCGGGGAC GACGATGACT CAAATTCCAT GCGACTTCAG 
CCAAGACAAA ACCAAGTTTC ATTCCAAGGG AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245291.1
CDS	1..4479
Translation	MYSQRTVQETKGWDVFREFPPKQDSVSMETQKCLEFTVRMLKVVAYLVTFIVVLGSGVIAKGTILFMTSQIRKDKRLEFCNKNLGRDKQFVVSLPDEERVAWMWAILAAFAIPELGTMIRSIRICFFKTSKKPSFVQFSVVFIAESLHTIGMGLLFFKILPELDVVKGAMITNCLCIIPAVLGLLSRNSRDSKRFVKVIVDMAAIVAQVTGFIVWPLLENKAVLWLIPISALCISLGWWENYVTRQSPIGIIKSLGRLKEELNFTRYFTYRFISVWKILLFLFCNLFFMWLDGDEPAMFFQLFNPGFGPHSIVVEEVQIQLGGTVIPDLANITLTGDSVEIAAVHKSAVYVMMIQIFAAYICYIFGKFACKIVIQGFSYAFPINLVIPLVVNFLIAACGIRNGDTCFFHGTIPDYLFFESPPVFTLSDFISRQMAWVWLLWLLSQTWITIHIWTPKAERLASTEKLFVLPMYNGLLIDQSMALNRKRDDHKDVKTEDLAEIEKEKGDEYYETISVHTDNTGSSPKTVKSSDQITRIYACATMWHETKDEMMEFLKSILRLDEDQCARRVAQKYLRVVDPDYYEFETHIFLDDAFEISDHSDDESQVNRFVKLLVDTIDEAASNVHQTNIRIRPPKRYPAPYGGRLTWVLPGKTKMICHLKDKAKIRHRKRWSQVMYMYYLLGHRLMELAISVDRKEVMAENTYLLTLDGDIDFQPHAVRLLIDLMKKNKNLGAACGRIHPVGSGPMVWYQLFEYAIGHWLQKATEHMIGCVLCSPGCFSLFRGKALMDDNVMKKYTLKSDEARHYVQYDQGEDRWLCTLLLQRGYRVEYSAASDAYTHCPEGFSEFYNQRRRWVPSTIANIMDLLVDYKHTIKINDNISSPYIAYQMMLMGGTILGPGTIFLMLVGAFVAAFRIDNWTSFEYNLYPIMLFMFVCFTMKSEYQEIEQEKKDAEEAKKKAKQKSLLGFLQGVNSNEEEGSIELSFAGLFKCLLCTHPKGNEEKVQLMHIASTLEKLEKKIENVEKAVDPHGLSRSRKLSLGPRGSTNGDHQLDALNEDPEEENDSDSDTGTLSTEPRERRDDLINPYWIEDPGLKKGEVDFLSPPEVQFWKDLIDKYLYPIDEDKDEKARISRDLKELRDSSVFSFFMINALFVLIVFLLQLNKDNLHIKWPFGVKTNITYDEVSQEARIAGDLLELRNKSVFAFVMFNALFILIVFLLQLNKDQLHVVWPLGVKTNITYMEETGQLLISKEYLQLEPIGLVFVFFFALILVIQFSAMLFHRFGTISHILSSTDLNWFCSKKSEDLSQDALLDKNAIAIVKDLQKLNGLDDDYDNDSGSGPHNVGRRKTIHNLEKARQKKRNIGTLDVAFKKRFFNMNANDGPGTPVLNRKMTLRRETLKALETRRNSVMAERRKSQMQTLGANNEYGVTGMLNNNVGPRHRASNANISVKDVFSEPNGGQINRAYEASLGDDDDSNSMRLQPRQNQVSFQGRF

Target ORF information:

RefSeq Version	NM_001258362.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori chitin synthase A (LOC100884166), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258362.1

ORF Insert Sequence:

ATGTATAGTC AGCGCACAGT ACAAGAAACG AAAGGATGGG ACGTGTTCAG AGAATTTCCG 
CCGAAACAGG ACAGTGTGTC CATGGAGACT CAGAAATGTT TGGAATTCAC AGTGCGAATG 
TTAAAAGTGG TCGCTTACCT GGTCACCTTC ATCGTGGTCC TTGGATCAGG AGTTATCGCA 
AAGGGGACAA TTTTGTTCAT GACGTCACAA ATTAGGAAAG ACAAGCGATT AGAATTCTGC 
AATAAAAACT TAGGTCGAGA TAAGCAGTTT GTGGTGAGTT TACCAGACGA GGAGCGTGTT 
GCCTGGATGT GGGCTATCCT AGCTGCCTTT GCTATTCCGG AATTAGGAAC GATGATCAGG 
TCTATAAGAA TATGCTTCTT CAAAACATCA AAGAAGCCGA GTTTTGTACA ATTTTCTGTG 
GTCTTTATCG CTGAGTCCTT ACATACCATA GGAATGGGTT TACTATTCTT TAAAATCCTG 
CCTGAATTAG ATGTAGTCAA AGGAGCTATG ATAACGAACT GTCTCTGTAT AATTCCCGCG 
GTGCTCGGTC TGCTGTCAAG AAACTCTCGA GACTCGAAAC GATTTGTCAA AGTCATCGTC 
GATATGGCCG CTATAGTTGC GCAAGTGACC GGTTTTATAG TTTGGCCCCT GTTGGAAAAC 
AAAGCTGTTT TGTGGTTGAT CCCGATCTCT GCTTTATGTA TTTCGTTAGG TTGGTGGGAA 
AACTATGTGA CACGACAAAG TCCAATAGGA ATAATCAAAA GCCTGGGGAG ACTAAAAGAG 
GAATTAAATT TTACTCGCTA CTTTACGTAT CGATTCATCT CCGTGTGGAA GATTTTACTG 
TTTCTTTTCT GCAATCTATT CTTCATGTGG TTGGACGGAG ATGAACCGGC AATGTTTTTC 
CAACTTTTCA ATCCTGGATT TGGGCCACAT AGCATTGTCG TTGAAGAGGT TCAAATCCAA 
CTCGGGGGAA CGGTTATTCC AGATTTGGCA AACATAACCC TTACAGGAGA CTCCGTCGAA 
ATCGCGGCTG TACATAAGTC CGCCGTCTAC GTCATGATGA TCCAAATATT TGCAGCCTAC 
ATCTGCTACA TCTTTGGGAA ATTTGCTTGC AAAATTGTTA TTCAAGGGTT CAGTTACGCT 
TTTCCGATAA ACTTGGTCAT ACCTTTGGTG GTGAACTTTT TGATTGCTGC TTGTGGTATC 
AGAAATGGCG ACACCTGTTT CTTCCACGGA ACGATACCGG ACTACCTATT CTTCGAAAGC 
CCTCCAGTGT TTACGTTGAG CGATTTCATC TCTCGACAAA TGGCATGGGT TTGGTTGTTA 
TGGCTGCTAT CACAAACCTG GATCACTATT CATATTTGGA CCCCCAAAGC AGAACGTTTA 
GCTTCCACTG AGAAATTGTT TGTGTTGCCT ATGTACAATG GTCTACTCAT AGACCAAAGT 
ATGGCACTGA ATAGGAAAAG AGATGACCAT AAGGATGTCA AAACAGAAGA TCTTGCCGAA 
ATCGAAAAGG AAAAAGGTGA TGAATACTAT GAAACAATAT CAGTACATAC AGACAATACT 
GGATCTTCCC CAAAGACTGT GAAATCATCA GACCAGATCA CGAGGATTTA TGCTTGTGCT 
ACTATGTGGC ACGAGACCAA AGATGAGATG ATGGAGTTCT TGAAATCTAT TCTTCGTCTC 
GATGAAGATC AGTGTGCTCG CCGCGTCGCT CAGAAGTATC TTCGTGTAGT AGATCCTGAC 
TATTACGAAT TCGAAACTCA CATTTTCTTG GACGACGCTT TCGAAATTTC GGATCATAGT 
GACGACGAAT CTCAAGTAAA CCGGTTTGTC AAGCTTCTGG TAGATACCAT CGACGAAGCT 
GCTTCTAATG TACATCAAAC GAACATTCGT ATACGTCCAC CGAAGAGATA TCCTGCGCCA 
TACGGAGGTA GACTGACATG GGTGTTACCA GGAAAGACTA AAATGATCTG TCACTTGAAG 
GATAAGGCAA AGATTCGACA CAGGAAACGT TGGTCTCAGG TGATGTACAT GTACTACCTT 
CTCGGTCATC GTCTAATGGA ATTGGCTATT TCGGTTGATC GTAAAGAAGT TATGGCTGAG 
AATACTTACC TGCTTACACT CGATGGAGAT ATCGATTTCC AACCTCACGC TGTACGATTA 
CTCATTGATT TAATGAAGAA AAACAAGAAC CTCGGAGCTG CCTGCGGGCG TATTCACCCT 
GTTGGATCTG GACCTATGGT GTGGTATCAA TTATTCGAGT ATGCTATCGG ACATTGGCTG 
CAAAAGGCCA CCGAACACAT GATCGGCTGC GTGCTCTGTA GTCCCGGATG CTTCTCGCTG 
TTCAGAGGAA AGGCTCTGAT GGACGACAAC GTTATGAAGA AATATACTTT GAAATCTGAC 
GAAGCCCGTC ACTACGTACA ATACGATCAG GGAGAAGATC GATGGCTATG CACTCTGCTA 
CTTCAACGTG GCTATCGTGT AGAATATTCA GCTGCATCGG ACGCCTACAC CCATTGCCCT 
GAAGGTTTTA GCGAGTTCTA CAATCAACGA CGTAGATGGG TGCCTTCGAC CATTGCTAAC 
ATTATGGACT TGCTTGTGGA TTATAAACAT ACCATTAAAA TCAATGACAA TATTTCCTCA 
CCGTACATCG CATACCAGAT GATGTTGATG GGCGGTACGA TTCTGGGACC TGGAACTATA 
TTTCTTATGT TGGTGGGTGC TTTTGTGGCC GCTTTTAGAA TTGATAATTG GACATCCTTC 
GAATACAATC TATATCCTAT AATGTTGTTT ATGTTTGTTT GTTTCACAAT GAAGTCAGAA 
TATCAAGAAA TCGAGCAAGA AAAGAAAGAT GCCGAAGAGG CGAAAAAGAA GGCGAAACAA 
AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC AATTCTAACG AAGAGGAAGG CTCCATAGAA 
CTATCGTTTG CTGGTCTTTT TAAATGTTTA CTGTGTACTC ATCCTAAAGG AAACGAAGAA 
AAAGTACAGC TCATGCATAT CGCTTCAACA CTTGAGAAAT TGGAAAAGAA AATTGAAAAC 
GTCGAGAAGG CCGTGGATCC GCACGGTCTA TCCAGAAGTC GCAAACTATC GCTAGGCCCT 
CGTGGTAGCA CTAACGGAGA CCACCAGTTG GACGCTCTTA ATGAGGACCC CGAAGAAGAA 
AACGATTCCG ATTCCGACAC CGGCACTCTA TCCACTGAAC CAAGGGAACG ACGAGATGAT 
CTTATAAATC CATATTGGAT TGAGGATCCC GGCTTGAAAA AAGGCGAAGT AGATTTCTTG 
AGCCCGCCCG AGGTACAATT CTGGAAAGAC CTTATTGACA AGTATTTATA TCCTATTGAC 
GAAGACAAGG ACGAGAAGGC ACGTATTTCG AGAGACTTAA AAGAACTGAG AGATTCTTCT 
GTATTTTCAT TCTTTATGAT CAATGCTCTT TTTGTGCTCA TTGTATTTTT GCTACAATTG 
AACAAGGACA ACCTTCACAT TAAATGGCCC TTCGGAGTTA AGACTAACAT TACCTATGAT 
GAGGTCTCAC AAGAGGCTCG CATAGCAGGC GATCTACTAG AACTTCGCAA TAAATCAGTT 
TTCGCATTTG TTATGTTCAA TGCTTTATTT ATATTGATAG TATTTTTATT ACAACTCAAT 
AAAGATCAGC TTCACGTGGT CTGGCCTTTG GGAGTTAAGA CAAACATTAC GTACATGGAG 
GAAACAGGCC AGCTTCTCAT CTCGAAGGAA TACTTGCAAT TAGAGCCTAT CGGTCTGGTA 
TTTGTGTTCT TCTTTGCCTT GATTTTGGTC ATACAATTTT CGGCCATGTT GTTCCATCGA 
TTCGGAACTA TTTCGCATAT TCTATCATCG ACGGATCTCA ACTGGTTCTG TTCAAAGAAA 
TCGGAAGATT TGTCACAGGA TGCGCTTTTG GATAAGAATG CAATAGCAAT AGTGAAAGAC 
CTTCAGAAAT TAAACGGTCT GGACGATGAT TATGATAACG ATTCAGGATC TGGACCACAC 
AACGTCGGCC GAAGGAAGAC AATACACAAT TTAGAGAAAG CTAGACAGAA GAAACGTAAC 
ATAGGAACAC TAGATGTGGC TTTCAAGAAA CGATTCTTCA ATATGAATGC TAATGATGGT 
CCGGGTACCC CAGTTTTAAA TCGTAAAATG ACATTGAGAA GGGAGACATT GAAAGCTTTA 
GAGACCCGTC GTAACTCTGT AATGGCAGAA AGAAGGAAGT CGCAAATGCA GACACTTGGC 
GCTAATAATG AATACGGTGT TACTGGAATG CTCAATAATA ACGTTGGACC TCGTCATAGA 
GCATCGAATG CTAATATTTC AGTGAAGGAT GTATTCTCGG AGCCCAACGG AGGACAGATC 
AACAGGGCCT ACGAAGCCTC ACTCGGGGAC GACGATGACT CAAATTCCAT GCGACTTCAG 
CCAAGACAAA ACCAAGTTTC ATTCCAAGGG AGGTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100884166 ( XM_012694437.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012549891.1 Bombyx mori chitin synthase A (LOC100884166), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBg14616
Clone ID Related Accession (Same CDS sequence)	XM_012694437.1
Accession Version	XM_012694437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	chitin synthase A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582020.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAC AAGAACGTAG GAGTCAATAC GAGAACGAGG AGACGGAGGA AGTCGTTTAC 
ATTCCAAATA ACAATTACTC GATATACAGT AATGGAACTT TGCATTACTG TAGCATATAC 
AGTCAGCGCA CAGTACAAGA AACGAAAGGA TGGGACGTGT TCAGAGAATT TCCGCCGAAA 
CAGGACAGTG TGTCCATGGA GACTCAGAAA TGTTTGGAAT TCACAGTGCG AATGTTAAAA 
GTGGTCGCTT ACCTGGTCAC CTTCATCGTG GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG 
ACAATTTTGT TCATGACGTC ACAAATTAGG AAAGACAAGC GATTAGAATT CTGCAATAAA 
AACTTAGGTC GAGATAAGCA GTTTGTGGTG AGTTTACCAG ACGAGGAGCG TGTTGCCTGG 
ATGTGGGCTA TCCTAGCTGC CTTTGCTATT CCGGAATTAG GAACGATGAT CAGGTCTATA 
AGAATATGCT TCTTCAAAAC ATCAAAGAAG CCGAGTTTTG TACAATTTTC TGTGGTCTTT 
ATCGCTGAGT CCTTACATAC CATAGGAATG GGTTTACTAT TCTTTAAAAT CCTGCCTGAA 
TTAGATGTAG TCAAAGGAGC TATGATAACG AACTGTCTCT GTATAATTCC CGCGGTGCTC 
GGTCTGCTGT CAAGAAACTC TCGAGACTCG AAACGATTTG TCAAAGTCAT CGTCGATATG 
GCCGCTATAG TTGCGCAAGT GACCGGTTTT ATAGTTTGGC CCCTGTTGGA AAACAAAGCT 
GTTTTGTGGT TGATCCCGAT CTCTGCTTTA TGTATTTCGT TAGGTTGGTG GGAAAACTAT 
GTGACACGAC AAAGTCCAAT AGGAATAATC AAAAGCCTGG GGAGACTAAA AGAGGAATTA 
AATTTTACTC GCTACTTTAC GTATCGATTC ATCTCCGTGT GGAAGATTTT ACTGTTTCTT 
TTCTGCAATC TATTCTTCAT GTGGTTGGAC GGAGATGAAC CGGCAATGTT TTTCCAACTT 
TTCAATCCTG GATTTGGGCC ACATAGCATT GTCGTTGAAG AGGTTCAAAT CCAACTCGGG 
GGAACGGTTA TTCCAGATTT GGCAAACATA ACCCTTACAG GAGACTCCGT CGAAATCGCG 
GCTGTACATA AGTCCGCCGT CTACGTCATG ATGATCCAAA TATTTGCAGC CTACATCTGC 
TACATCTTTG GGAAATTTGC TTGCAAAATT GTTATTCAAG GGTTCAGTTA CGCTTTTCCG 
ATAAACTTGG TCATACCTTT GGTGGTGAAC TTTTTGATTG CTGCTTGTGG TATCAGAAAT 
GGCGACACCT GTTTCTTCCA CGGAACGATA CCGGACTACC TATTCTTCGA AAGCCCTCCA 
GTGTTTACGT TGAGCGATTT CATCTCTCGA CAAATGGCAT GGGTTTGGTT GTTATGGCTG 
CTATCACAAA CCTGGATCAC TATTCATATT TGGACCCCCA AAGCAGAACG TTTAGCTTCC 
ACTGAGAAAT TGTTTGTGTT GCCTATGTAC AATGGTCTAC TCATAGACCA AAGTATGGCA 
CTGAATAGGA AAAGAGATGA CCATAAGGAT GTCAAAACAG AAGATCTTGC CGAAATCGAA 
AAGGAAAAAG GTGATGAATA CTATGAAACA ATATCAGTAC ATACAGACAA TACTGGATCT 
TCCCCAAAGA CTGTGAAATC ATCAGACCAG ATCACGAGGA TTTATGCTTG TGCTACTATG 
TGGCACGAGA CCAAAGATGA GATGATGGAG TTCTTGAAAT CTATTCTTCG TCTCGATGAA 
GATCAGTGTG CTCGCCGCGT CGCTCAGAAG TATCTTCGTG TAGTAGATCC TGACTATTAC 
GAATTCGAAA CTCACATTTT CTTGGACGAC GCTTTCGAAA TTTCGGATCA TAGTGACGAC 
GAATCTCAAG TAAACCGGTT TGTCAAGCTT CTGGTAGATA CCATCGACGA AGCTGCTTCT 
AATGTACATC AAACGAACAT TCGTATACGT CCACCGAAGA GATATCCTGC GCCATACGGA 
GGTAGACTGA CATGGGTGTT ACCAGGAAAG ACTAAAATGA TCTGTCACTT GAAGGATAAG 
GCAAAGATTC GACACAGGAA ACGTTGGTCT CAGGTGATGT ACATGTACTA CCTTCTCGGT 
CATCGTCTAA TGGAATTGGC TATTTCGGTT GATCGTAAAG AAGTTATGGC TGAGAATACT 
TACCTGCTTA CACTCGATGG AGATATCGAT TTCCAACCTC ACGCTGTACG ATTACTCATT 
GATTTAATGA AGAAAAACAA GAACCTCGGA GCTGCCTGCG GGCGTATTCA CCCTGTTGGA 
TCTGGACCTA TGGTGTGGTA TCAATTATTC GAGTATGCTA TCGGACATTG GCTGCAAAAG 
GCCACCGAAC ACATGATCGG CTGCGTGCTC TGTAGTCCCG GATGCTTCTC GCTGTTCAGA 
GGAAAGGCTC TGATGGACGA CAACGTTATG AAGAAATATA CTTTGAAATC TGACGAAGCC 
CGTCACTACG TACAATACGA TCAGGGAGAA GATCGATGGC TATGCACTCT GCTACTTCAA 
CGTGGCTATC GTGTAGAATA TTCAGCTGCA TCGGACGCCT ACACCCATTG CCCTGAAGGT 
TTTAGCGAGT TCTACAATCA ACGACGTAGA TGGGTGCCTT CGACCATTGC TAACATTATG 
GACTTGCTTG TGGATTATAA ACATACCATT AAAATCAATG ACAATATTTC CTCACCGTAC 
ATCGCATACC AGATGATGTT GATGGGCGGT ACGATTCTGG GACCTGGAAC TATATTTCTT 
ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT AGAATTGATA ATTGGACATC CTTCGAATAC 
AATCTATATC CTATAATGTT GTTTATGTTT GTTTGTTTCA CAATGAAGTC AGAATATCAA 
TTATTGGTCG CGCAAATATT ATCAACGGCC TATGCTATGA TAATGATGGC TGTAATTGTG 
GGTACAGCAC TGCAATTAGG CGAGGACGGT ATCGGATCCC CATCCGCTAT ATTCTTGATT 
TCACTGTCAA GTTCTTTCTT TATAGCGGCC TGTTTACATC CCCAGGAGTT TTGGTGTATC 
GTTCCCGGTA TCATTTATCT ATTATCTATA CCCTCTATGT ACTTGCTTTT GATTTTGTAT 
TCTATTATAA ATCTGAATGT AGTATCGTGG GGTACGCGAG AAGTGCAAAC CAAGAAAACA 
AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT GCCGAAGAGG CGAAAAAGAA GGCGAAACAA 
AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC AATTCTAACG AAGAGGAAGG CTCCATAGAA 
CTATCGTTTG CTGGTCTTTT TAAATGTTTA CTGTGTACTC ATCCTAAAGG AAACGAAGAA 
AAAGTACAGC TCATGCATAT CGCTTCAACA CTTGAGAAAT TGGAAAAGAA AATTGAAAAC 
GTCGAGAAGG CCGTGGATCC GCACGGTCTA TCCAGAAGTC GCAAACTATC GCTAGGCCCT 
CGTGGTAGCA CTAACGGAGA CCACCAGTTG GACGCTCTTA ATGAGGACCC CGAAGAAGAA 
AACGATTCCG ATTCCGACAC CGGCACTCTA TCCACTGAAC CAAGGGAACG ACGAGATGAT 
CTTATAAATC CATATTGGAT TGAGGATCCC GGCTTGAAAA AAGGCGAAGT AGATTTCTTG 
AGCCCGCCCG AGGTACAATT CTGGAAAGAC CTTATTGACA AGTATTTATA TCCTATTGAC 
GAAGACAAGG ACGAGAAGGC TCGCATAGCA GGCGATCTAC TAGAACTTCG CAATAAATCA 
GTTTTCGCAT TTGTTATGTT CAATGCTTTA TTTATATTGA TAGTATTTTT ATTACAACTC 
AATAAAGATC AGCTTCACGT GGTCTGGCCT TTGGGAGTTA AGACAAACAT TACGTACATG 
GAGGAAACAG GCCAGCTTCT CATCTCGAAG GAATACTTGC AATTAGAGCC TATCGGTCTG 
GTATTTGTGT TCTTCTTTGC CTTGATTTTG GTCATACAAT TTTCGGCCAT GTTGTTCCAT 
CGATTCGGAA CTATTTCGCA TATTCTATCA TCGACGGATC TCAACTGGTT CTGTTCAAAG 
AAATCGGAAG ATTTGTCACA GGATGCGCTT TTGGATAAGA ATGCAATAGC AATAGTGAAA 
GACCTTCAGA AATTAAACGG TCTGGACGAT GATTATGATA ACGATTCAGG ATCTGGACCA 
CACAACGTCG GCCGAAGGAA GACAATACAC AATTTAGAGA AAGCTAGACA GAAGAAACGT 
AACATAGGAA CACTAGATGT GGCTTTCAAG AAACGATTCT TCAATATGAA TGCTAATGAT 
GGTCCGGGTA CCCCAGTTTT AAATCGTAAA ATGACATTGA GAAGGGAGAC ATTGAAAGCT 
TTAGAGACCC GTCGTAACTC TGTAATGGCA GAAAGAAGGA AGTCGCAAAT GCAGACACTT 
GGCGCTAATA ATGAATACGG TGTTACTGGA ATGCTCAATA ATAACGTTGG ACCTCGTCAT 
AGAGCATCGA ATGCTAATAT TTCAGTGAAG GATGTATTCT CGGAGCCCAA CGGAGGACAG 
ATCAACAGGG CCTACGAAGC CTCACTCGGG GACGACGATG ACTCAAATTC CATGCGACTT 
CAGCCAAGAC AAAACCAAGT TTCATTCCAA GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012549891.1
CDS	107..4828
Translation	MSEQERRSQYENEETEEVVYIPNNNYSIYSNGTLHYCSIYSQRTVQETKGWDVFREFPPKQDSVSMETQKCLEFTVRMLKVVAYLVTFIVVLGSGVIAKGTILFMTSQIRKDKRLEFCNKNLGRDKQFVVSLPDEERVAWMWAILAAFAIPELGTMIRSIRICFFKTSKKPSFVQFSVVFIAESLHTIGMGLLFFKILPELDVVKGAMITNCLCIIPAVLGLLSRNSRDSKRFVKVIVDMAAIVAQVTGFIVWPLLENKAVLWLIPISALCISLGWWENYVTRQSPIGIIKSLGRLKEELNFTRYFTYRFISVWKILLFLFCNLFFMWLDGDEPAMFFQLFNPGFGPHSIVVEEVQIQLGGTVIPDLANITLTGDSVEIAAVHKSAVYVMMIQIFAAYICYIFGKFACKIVIQGFSYAFPINLVIPLVVNFLIAACGIRNGDTCFFHGTIPDYLFFESPPVFTLSDFISRQMAWVWLLWLLSQTWITIHIWTPKAERLASTEKLFVLPMYNGLLIDQSMALNRKRDDHKDVKTEDLAEIEKEKGDEYYETISVHTDNTGSSPKTVKSSDQITRIYACATMWHETKDEMMEFLKSILRLDEDQCARRVAQKYLRVVDPDYYEFETHIFLDDAFEISDHSDDESQVNRFVKLLVDTIDEAASNVHQTNIRIRPPKRYPAPYGGRLTWVLPGKTKMICHLKDKAKIRHRKRWSQVMYMYYLLGHRLMELAISVDRKEVMAENTYLLTLDGDIDFQPHAVRLLIDLMKKNKNLGAACGRIHPVGSGPMVWYQLFEYAIGHWLQKATEHMIGCVLCSPGCFSLFRGKALMDDNVMKKYTLKSDEARHYVQYDQGEDRWLCTLLLQRGYRVEYSAASDAYTHCPEGFSEFYNQRRRWVPSTIANIMDLLVDYKHTIKINDNISSPYIAYQMMLMGGTILGPGTIFLMLVGAFVAAFRIDNWTSFEYNLYPIMLFMFVCFTMKSEYQLLVAQILSTAYAMIMMAVIVGTALQLGEDGIGSPSAIFLISLSSSFFIAACLHPQEFWCIVPGIIYLLSIPSMYLLLILYSIINLNVVSWGTREVQTKKTKKEIEQEKKDAEEAKKKAKQKSLLGFLQGVNSNEEEGSIELSFAGLFKCLLCTHPKGNEEKVQLMHIASTLEKLEKKIENVEKAVDPHGLSRSRKLSLGPRGSTNGDHQLDALNEDPEEENDSDSDTGTLSTEPRERRDDLINPYWIEDPGLKKGEVDFLSPPEVQFWKDLIDKYLYPIDEDKDEKARIAGDLLELRNKSVFAFVMFNALFILIVFLLQLNKDQLHVVWPLGVKTNITYMEETGQLLISKEYLQLEPIGLVFVFFFALILVIQFSAMLFHRFGTISHILSSTDLNWFCSKKSEDLSQDALLDKNAIAIVKDLQKLNGLDDDYDNDSGSGPHNVGRRKTIHNLEKARQKKRNIGTLDVAFKKRFFNMNANDGPGTPVLNRKMTLRRETLKALETRRNSVMAERRKSQMQTLGANNEYGVTGMLNNNVGPRHRASNANISVKDVFSEPNGGQINRAYEASLGDDDDSNSMRLQPRQNQVSFQGRF

Target ORF information:

RefSeq Version	XM_012694437.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori chitin synthase A (LOC100884166), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012694437.1

ORF Insert Sequence:

ATGAGTGAAC AAGAACGTAG GAGTCAATAC GAGAACGAGG AGACGGAGGA AGTCGTTTAC 
ATTCCAAATA ACAATTACTC GATATACAGT AATGGAACTT TGCATTACTG TAGCATATAC 
AGTCAGCGCA CAGTACAAGA AACGAAAGGA TGGGACGTGT TCAGAGAATT TCCGCCGAAA 
CAGGACAGTG TGTCCATGGA GACTCAGAAA TGTTTGGAAT TCACAGTGCG AATGTTAAAA 
GTGGTCGCTT ACCTGGTCAC CTTCATCGTG GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG 
ACAATTTTGT TCATGACGTC ACAAATTAGG AAAGACAAGC GATTAGAATT CTGCAATAAA 
AACTTAGGTC GAGATAAGCA GTTTGTGGTG AGTTTACCAG ACGAGGAGCG TGTTGCCTGG 
ATGTGGGCTA TCCTAGCTGC CTTTGCTATT CCGGAATTAG GAACGATGAT CAGGTCTATA 
AGAATATGCT TCTTCAAAAC ATCAAAGAAG CCGAGTTTTG TACAATTTTC TGTGGTCTTT 
ATCGCTGAGT CCTTACATAC CATAGGAATG GGTTTACTAT TCTTTAAAAT CCTGCCTGAA 
TTAGATGTAG TCAAAGGAGC TATGATAACG AACTGTCTCT GTATAATTCC CGCGGTGCTC 
GGTCTGCTGT CAAGAAACTC TCGAGACTCG AAACGATTTG TCAAAGTCAT CGTCGATATG 
GCCGCTATAG TTGCGCAAGT GACCGGTTTT ATAGTTTGGC CCCTGTTGGA AAACAAAGCT 
GTTTTGTGGT TGATCCCGAT CTCTGCTTTA TGTATTTCGT TAGGTTGGTG GGAAAACTAT 
GTGACACGAC AAAGTCCAAT AGGAATAATC AAAAGCCTGG GGAGACTAAA AGAGGAATTA 
AATTTTACTC GCTACTTTAC GTATCGATTC ATCTCCGTGT GGAAGATTTT ACTGTTTCTT 
TTCTGCAATC TATTCTTCAT GTGGTTGGAC GGAGATGAAC CGGCAATGTT TTTCCAACTT 
TTCAATCCTG GATTTGGGCC ACATAGCATT GTCGTTGAAG AGGTTCAAAT CCAACTCGGG 
GGAACGGTTA TTCCAGATTT GGCAAACATA ACCCTTACAG GAGACTCCGT CGAAATCGCG 
GCTGTACATA AGTCCGCCGT CTACGTCATG ATGATCCAAA TATTTGCAGC CTACATCTGC 
TACATCTTTG GGAAATTTGC TTGCAAAATT GTTATTCAAG GGTTCAGTTA CGCTTTTCCG 
ATAAACTTGG TCATACCTTT GGTGGTGAAC TTTTTGATTG CTGCTTGTGG TATCAGAAAT 
GGCGACACCT GTTTCTTCCA CGGAACGATA CCGGACTACC TATTCTTCGA AAGCCCTCCA 
GTGTTTACGT TGAGCGATTT CATCTCTCGA CAAATGGCAT GGGTTTGGTT GTTATGGCTG 
CTATCACAAA CCTGGATCAC TATTCATATT TGGACCCCCA AAGCAGAACG TTTAGCTTCC 
ACTGAGAAAT TGTTTGTGTT GCCTATGTAC AATGGTCTAC TCATAGACCA AAGTATGGCA 
CTGAATAGGA AAAGAGATGA CCATAAGGAT GTCAAAACAG AAGATCTTGC CGAAATCGAA 
AAGGAAAAAG GTGATGAATA CTATGAAACA ATATCAGTAC ATACAGACAA TACTGGATCT 
TCCCCAAAGA CTGTGAAATC ATCAGACCAG ATCACGAGGA TTTATGCTTG TGCTACTATG 
TGGCACGAGA CCAAAGATGA GATGATGGAG TTCTTGAAAT CTATTCTTCG TCTCGATGAA 
GATCAGTGTG CTCGCCGCGT CGCTCAGAAG TATCTTCGTG TAGTAGATCC TGACTATTAC 
GAATTCGAAA CTCACATTTT CTTGGACGAC GCTTTCGAAA TTTCGGATCA TAGTGACGAC 
GAATCTCAAG TAAACCGGTT TGTCAAGCTT CTGGTAGATA CCATCGACGA AGCTGCTTCT 
AATGTACATC AAACGAACAT TCGTATACGT CCACCGAAGA GATATCCTGC GCCATACGGA 
GGTAGACTGA CATGGGTGTT ACCAGGAAAG ACTAAAATGA TCTGTCACTT GAAGGATAAG 
GCAAAGATTC GACACAGGAA ACGTTGGTCT CAGGTGATGT ACATGTACTA CCTTCTCGGT 
CATCGTCTAA TGGAATTGGC TATTTCGGTT GATCGTAAAG AAGTTATGGC TGAGAATACT 
TACCTGCTTA CACTCGATGG AGATATCGAT TTCCAACCTC ACGCTGTACG ATTACTCATT 
GATTTAATGA AGAAAAACAA GAACCTCGGA GCTGCCTGCG GGCGTATTCA CCCTGTTGGA 
TCTGGACCTA TGGTGTGGTA TCAATTATTC GAGTATGCTA TCGGACATTG GCTGCAAAAG 
GCCACCGAAC ACATGATCGG CTGCGTGCTC TGTAGTCCCG GATGCTTCTC GCTGTTCAGA 
GGAAAGGCTC TGATGGACGA CAACGTTATG AAGAAATATA CTTTGAAATC TGACGAAGCC 
CGTCACTACG TACAATACGA TCAGGGAGAA GATCGATGGC TATGCACTCT GCTACTTCAA 
CGTGGCTATC GTGTAGAATA TTCAGCTGCA TCGGACGCCT ACACCCATTG CCCTGAAGGT 
TTTAGCGAGT TCTACAATCA ACGACGTAGA TGGGTGCCTT CGACCATTGC TAACATTATG 
GACTTGCTTG TGGATTATAA ACATACCATT AAAATCAATG ACAATATTTC CTCACCGTAC 
ATCGCATACC AGATGATGTT GATGGGCGGT ACGATTCTGG GACCTGGAAC TATATTTCTT 
ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT AGAATTGATA ATTGGACATC CTTCGAATAC 
AATCTATATC CTATAATGTT GTTTATGTTT GTTTGTTTCA CAATGAAGTC AGAATATCAA 
TTATTGGTCG CGCAAATATT ATCAACGGCC TATGCTATGA TAATGATGGC TGTAATTGTG 
GGTACAGCAC TGCAATTAGG CGAGGACGGT ATCGGATCCC CATCCGCTAT ATTCTTGATT 
TCACTGTCAA GTTCTTTCTT TATAGCGGCC TGTTTACATC CCCAGGAGTT TTGGTGTATC 
GTTCCCGGTA TCATTTATCT ATTATCTATA CCCTCTATGT ACTTGCTTTT GATTTTGTAT 
TCTATTATAA ATCTGAATGT AGTATCGTGG GGTACGCGAG AAGTGCAAAC CAAGAAAACA 
AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT GCCGAAGAGG CGAAAAAGAA GGCGAAACAA 
AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC AATTCTAACG AAGAGGAAGG CTCCATAGAA 
CTATCGTTTG CTGGTCTTTT TAAATGTTTA CTGTGTACTC ATCCTAAAGG AAACGAAGAA 
AAAGTACAGC TCATGCATAT CGCTTCAACA CTTGAGAAAT TGGAAAAGAA AATTGAAAAC 
GTCGAGAAGG CCGTGGATCC GCACGGTCTA TCCAGAAGTC GCAAACTATC GCTAGGCCCT 
CGTGGTAGCA CTAACGGAGA CCACCAGTTG GACGCTCTTA ATGAGGACCC CGAAGAAGAA 
AACGATTCCG ATTCCGACAC CGGCACTCTA TCCACTGAAC CAAGGGAACG ACGAGATGAT 
CTTATAAATC CATATTGGAT TGAGGATCCC GGCTTGAAAA AAGGCGAAGT AGATTTCTTG 
AGCCCGCCCG AGGTACAATT CTGGAAAGAC CTTATTGACA AGTATTTATA TCCTATTGAC 
GAAGACAAGG ACGAGAAGGC TCGCATAGCA GGCGATCTAC TAGAACTTCG CAATAAATCA 
GTTTTCGCAT TTGTTATGTT CAATGCTTTA TTTATATTGA TAGTATTTTT ATTACAACTC 
AATAAAGATC AGCTTCACGT GGTCTGGCCT TTGGGAGTTA AGACAAACAT TACGTACATG 
GAGGAAACAG GCCAGCTTCT CATCTCGAAG GAATACTTGC AATTAGAGCC TATCGGTCTG 
GTATTTGTGT TCTTCTTTGC CTTGATTTTG GTCATACAAT TTTCGGCCAT GTTGTTCCAT 
CGATTCGGAA CTATTTCGCA TATTCTATCA TCGACGGATC TCAACTGGTT CTGTTCAAAG 
AAATCGGAAG ATTTGTCACA GGATGCGCTT TTGGATAAGA ATGCAATAGC AATAGTGAAA 
GACCTTCAGA AATTAAACGG TCTGGACGAT GATTATGATA ACGATTCAGG ATCTGGACCA 
CACAACGTCG GCCGAAGGAA GACAATACAC AATTTAGAGA AAGCTAGACA GAAGAAACGT 
AACATAGGAA CACTAGATGT GGCTTTCAAG AAACGATTCT TCAATATGAA TGCTAATGAT 
GGTCCGGGTA CCCCAGTTTT AAATCGTAAA ATGACATTGA GAAGGGAGAC ATTGAAAGCT 
TTAGAGACCC GTCGTAACTC TGTAATGGCA GAAAGAAGGA AGTCGCAAAT GCAGACACTT 
GGCGCTAATA ATGAATACGG TGTTACTGGA ATGCTCAATA ATAACGTTGG ACCTCGTCAT 
AGAGCATCGA ATGCTAATAT TTCAGTGAAG GATGTATTCT CGGAGCCCAA CGGAGGACAG 
ATCAACAGGG CCTACGAAGC CTCACTCGGG GACGACGATG ACTCAAATTC CATGCGACTT 
CAGCCAAGAC AAAACCAAGT TTCATTCCAA GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100884166 ( XM_012694438.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012549892.1 Bombyx mori chitin synthase A (LOC100884166), transcript variant X2, mRNA.

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CloneID	OBg14617
Clone ID Related Accession (Same CDS sequence)	XM_012694438.1
Accession Version	XM_012694438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	chitin synthase A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGT CTGGAGGGAA ACGGCGAGAT GAGGGTAGCG ATAACTCTGA CGATGAACTC 
ACACCCCTGG CGAACGAAAT ATATGGTGGA AGTCAGCGCA CAGTACAAGA AACGAAAGGA 
TGGGACGTGT TCAGAGAATT TCCGCCGAAA CAGGACAGTG TGTCCATGGA GACTCAGAAA 
TGTTTGGAAT TCACAGTGCG AATGTTAAAA GTGGTCGCTT ACCTGGTCAC CTTCATCGTG 
GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG ACAATTTTGT TCATGACGTC ACAAATTAGG 
AAAGACAAGC GATTAGAATT CTGCAATAAA AACTTAGGTC GAGATAAGCA GTTTGTGGTG 
AGTTTACCAG ACGAGGAGCG TGTTGCCTGG ATGTGGGCTA TCCTAGCTGC CTTTGCTATT 
CCGGAATTAG GAACGATGAT CAGGTCTATA AGAATATGCT TCTTCAAAAC ATCAAAGAAG 
CCGAGTTTTG TACAATTTTC TGTGGTCTTT ATCGCTGAGT CCTTACATAC CATAGGAATG 
GGTTTACTAT TCTTTAAAAT CCTGCCTGAA TTAGATGTAG TCAAAGGAGC TATGATAACG 
AACTGTCTCT GTATAATTCC CGCGGTGCTC GGTCTGCTGT CAAGAAACTC TCGAGACTCG 
AAACGATTTG TCAAAGTCAT CGTCGATATG GCCGCTATAG TTGCGCAAGT GACCGGTTTT 
ATAGTTTGGC CCCTGTTGGA AAACAAAGCT GTTTTGTGGT TGATCCCGAT CTCTGCTTTA 
TGTATTTCGT TAGGTTGGTG GGAAAACTAT GTGACACGAC AAAGTCCAAT AGGAATAATC 
AAAAGCCTGG GGAGACTAAA AGAGGAATTA AATTTTACTC GCTACTTTAC GTATCGATTC 
ATCTCCGTGT GGAAGATTTT ACTGTTTCTT TTCTGCAATC TATTCTTCAT GTGGTTGGAC 
GGAGATGAAC CGGCAATGTT TTTCCAACTT TTCAATCCTG GATTTGGGCC ACATAGCATT 
GTCGTTGAAG AGGTTCAAAT CCAACTCGGG GGAACGGTTA TTCCAGATTT GGCAAACATA 
ACCCTTACAG GAGACTCCGT CGAAATCGCG GCTGTACATA AGTCCGCCGT CTACGTCATG 
ATGATCCAAA TATTTGCAGC CTACATCTGC TACATCTTTG GGAAATTTGC TTGCAAAATT 
GTTATTCAAG GGTTCAGTTA CGCTTTTCCG ATAAACTTGG TCATACCTTT GGTGGTGAAC 
TTTTTGATTG CTGCTTGTGG TATCAGAAAT GGCGACACCT GTTTCTTCCA CGGAACGATA 
CCGGACTACC TATTCTTCGA AAGCCCTCCA GTGTTTACGT TGAGCGATTT CATCTCTCGA 
CAAATGGCAT GGGTTTGGTT GTTATGGCTG CTATCACAAA CCTGGATCAC TATTCATATT 
TGGACCCCCA AAGCAGAACG TTTAGCTTCC ACTGAGAAAT TGTTTGTGTT GCCTATGTAC 
AATGGTCTAC TCATAGACCA AAGTATGGCA CTGAATAGGA AAAGAGATGA CCATAAGGAT 
GTCAAAACAG AAGATCTTGC CGAAATCGAA AAGGAAAAAG GTGATGAATA CTATGAAACA 
ATATCAGTAC ATACAGACAA TACTGGATCT TCCCCAAAGA CTGTGAAATC ATCAGACCAG 
ATCACGAGGA TTTATGCTTG TGCTACTATG TGGCACGAGA CCAAAGATGA GATGATGGAG 
TTCTTGAAAT CTATTCTTCG TCTCGATGAA GATCAGTGTG CTCGCCGCGT CGCTCAGAAG 
TATCTTCGTG TAGTAGATCC TGACTATTAC GAATTCGAAA CTCACATTTT CTTGGACGAC 
GCTTTCGAAA TTTCGGATCA TAGTGACGAC GAATCTCAAG TAAACCGGTT TGTCAAGCTT 
CTGGTAGATA CCATCGACGA AGCTGCTTCT AATGTACATC AAACGAACAT TCGTATACGT 
CCACCGAAGA GATATCCTGC GCCATACGGA GGTAGACTGA CATGGGTGTT ACCAGGAAAG 
ACTAAAATGA TCTGTCACTT GAAGGATAAG GCAAAGATTC GACACAGGAA ACGTTGGTCT 
CAGGTGATGT ACATGTACTA CCTTCTCGGT CATCGTCTAA TGGAATTGGC TATTTCGGTT 
GATCGTAAAG AAGTTATGGC TGAGAATACT TACCTGCTTA CACTCGATGG AGATATCGAT 
TTCCAACCTC ACGCTGTACG ATTACTCATT GATTTAATGA AGAAAAACAA GAACCTCGGA 
GCTGCCTGCG GGCGTATTCA CCCTGTTGGA TCTGGACCTA TGGTGTGGTA TCAATTATTC 
GAGTATGCTA TCGGACATTG GCTGCAAAAG GCCACCGAAC ACATGATCGG CTGCGTGCTC 
TGTAGTCCCG GATGCTTCTC GCTGTTCAGA GGAAAGGCTC TGATGGACGA CAACGTTATG 
AAGAAATATA CTTTGAAATC TGACGAAGCC CGTCACTACG TACAATACGA TCAGGGAGAA 
GATCGATGGC TATGCACTCT GCTACTTCAA CGTGGCTATC GTGTAGAATA TTCAGCTGCA 
TCGGACGCCT ACACCCATTG CCCTGAAGGT TTTAGCGAGT TCTACAATCA ACGACGTAGA 
TGGGTGCCTT CGACCATTGC TAACATTATG GACTTGCTTG TGGATTATAA ACATACCATT 
AAAATCAATG ACAATATTTC CTCACCGTAC ATCGCATACC AGATGATGTT GATGGGCGGT 
ACGATTCTGG GACCTGGAAC TATATTTCTT ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT 
AGAATTGATA ATTGGACATC CTTCGAATAC AATCTATATC CTATAATGTT GTTTATGTTT 
GTTTGTTTCA CAATGAAGTC AGAATATCAA TTATTGGTCG CGCAAATATT ATCAACGGCC 
TATGCTATGA TAATGATGGC TGTAATTGTG GGTACAGCAC TGCAATTAGG CGAGGACGGT 
ATCGGATCCC CATCCGCTAT ATTCTTGATT TCACTGTCAA GTTCTTTCTT TATAGCGGCC 
TGTTTACATC CCCAGGAGTT TTGGTGTATC GTTCCCGGTA TCATTTATCT ATTATCTATA 
CCCTCTATGT ACTTGCTTTT GATTTTGTAT TCTATTATAA ATCTGAATGT AGTATCGTGG 
GGTACGCGAG AAGTGCAAAC CAAGAAAACA AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT 
GCCGAAGAGG CGAAAAAGAA GGCGAAACAA AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC 
AATTCTAACG AAGAGGAAGG CTCCATAGAA CTATCGTTTG CTGGTCTTTT TAAATGTTTA 
CTGTGTACTC ATCCTAAAGG AAACGAAGAA AAAGTACAGC TCATGCATAT CGCTTCAACA 
CTTGAGAAAT TGGAAAAGAA AATTGAAAAC GTCGAGAAGG CCGTGGATCC GCACGGTCTA 
TCCAGAAGTC GCAAACTATC GCTAGGCCCT CGTGGTAGCA CTAACGGAGA CCACCAGTTG 
GACGCTCTTA ATGAGGACCC CGAAGAAGAA AACGATTCCG ATTCCGACAC CGGCACTCTA 
TCCACTGAAC CAAGGGAACG ACGAGATGAT CTTATAAATC CATATTGGAT TGAGGATCCC 
GGCTTGAAAA AAGGCGAAGT AGATTTCTTG AGCCCGCCCG AGGTACAATT CTGGAAAGAC 
CTTATTGACA AGTATTTATA TCCTATTGAC GAAGACAAGG ACGAGAAGGC ACGTATTTCG 
AGAGACTTAA AAGAACTGAG AGATTCTTCT GTATTTTCAT TCTTTATGAT CAATGCTCTT 
TTTGTGCTCA TTGTATTTTT GCTACAATTG AACAAGGACA ACCTTCACAT TAAATGGCCC 
TTCGGAGTTA AGACTAACAT TACCTATGAT GAGGTCTCAC AAGAGCTTCT CATCTCGAAG 
GAATACTTGC AATTAGAGCC TATCGGTCTG GTATTTGTGT TCTTCTTTGC CTTGATTTTG 
GTCATACAAT TTTCGGCCAT GTTGTTCCAT CGATTCGGAA CTATTTCGCA TATTCTATCA 
TCGACGGATC TCAACTGGTT CTGTTCAAAG AAATCGGAAG ATTTGTCACA GGATGCGCTT 
TTGGATAAGA ATGCAATAGC AATAGTGAAA GACCTTCAGA AATTAAACGG TCTGGACGAT 
GATTATGATA ACGATTCAGG ATCTGGACCA CACAACGTCG GCCGAAGGAA GACAATACAC 
AATTTAGAGA AAGCTAGACA GAAGAAACGT AACATAGGAA CACTAGATGT GGCTTTCAAG 
AAACGATTCT TCAATATGAA TGCTAATGAT GGTCCGGGTA CCCCAGTTTT AAATCGTAAA 
ATGACATTGA GAAGGGAGAC ATTGAAAGCT TTAGAGACCC GTCGTAACTC TGTAATGGCA 
GAAAGAAGGA AGTCGCAAAT GCAGACACTT GGCGCTAATA ATGAATACGG TGTTACTGGA 
ATGCTCAATA ATAACGTTGG ACCTCGTCAT AGAGCATCGA ATGCTAATAT TTCAGTGAAG 
GATGTATTCT CGGAGCCCAA CGGAGGACAG ATCAACAGGG CCTACGAAGC CTCACTCGGG 
GACGACGATG ACTCAAATTC CATGCGACTT CAGCCAAGAC AAAACCAAGT TTCATTCCAA 
GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012549892.1
CDS	154..4845
Translation	MATSGGKRRDEGSDNSDDELTPLANEIYGGSQRTVQETKGWDVFREFPPKQDSVSMETQKCLEFTVRMLKVVAYLVTFIVVLGSGVIAKGTILFMTSQIRKDKRLEFCNKNLGRDKQFVVSLPDEERVAWMWAILAAFAIPELGTMIRSIRICFFKTSKKPSFVQFSVVFIAESLHTIGMGLLFFKILPELDVVKGAMITNCLCIIPAVLGLLSRNSRDSKRFVKVIVDMAAIVAQVTGFIVWPLLENKAVLWLIPISALCISLGWWENYVTRQSPIGIIKSLGRLKEELNFTRYFTYRFISVWKILLFLFCNLFFMWLDGDEPAMFFQLFNPGFGPHSIVVEEVQIQLGGTVIPDLANITLTGDSVEIAAVHKSAVYVMMIQIFAAYICYIFGKFACKIVIQGFSYAFPINLVIPLVVNFLIAACGIRNGDTCFFHGTIPDYLFFESPPVFTLSDFISRQMAWVWLLWLLSQTWITIHIWTPKAERLASTEKLFVLPMYNGLLIDQSMALNRKRDDHKDVKTEDLAEIEKEKGDEYYETISVHTDNTGSSPKTVKSSDQITRIYACATMWHETKDEMMEFLKSILRLDEDQCARRVAQKYLRVVDPDYYEFETHIFLDDAFEISDHSDDESQVNRFVKLLVDTIDEAASNVHQTNIRIRPPKRYPAPYGGRLTWVLPGKTKMICHLKDKAKIRHRKRWSQVMYMYYLLGHRLMELAISVDRKEVMAENTYLLTLDGDIDFQPHAVRLLIDLMKKNKNLGAACGRIHPVGSGPMVWYQLFEYAIGHWLQKATEHMIGCVLCSPGCFSLFRGKALMDDNVMKKYTLKSDEARHYVQYDQGEDRWLCTLLLQRGYRVEYSAASDAYTHCPEGFSEFYNQRRRWVPSTIANIMDLLVDYKHTIKINDNISSPYIAYQMMLMGGTILGPGTIFLMLVGAFVAAFRIDNWTSFEYNLYPIMLFMFVCFTMKSEYQLLVAQILSTAYAMIMMAVIVGTALQLGEDGIGSPSAIFLISLSSSFFIAACLHPQEFWCIVPGIIYLLSIPSMYLLLILYSIINLNVVSWGTREVQTKKTKKEIEQEKKDAEEAKKKAKQKSLLGFLQGVNSNEEEGSIELSFAGLFKCLLCTHPKGNEEKVQLMHIASTLEKLEKKIENVEKAVDPHGLSRSRKLSLGPRGSTNGDHQLDALNEDPEEENDSDSDTGTLSTEPRERRDDLINPYWIEDPGLKKGEVDFLSPPEVQFWKDLIDKYLYPIDEDKDEKARISRDLKELRDSSVFSFFMINALFVLIVFLLQLNKDNLHIKWPFGVKTNITYDEVSQELLISKEYLQLEPIGLVFVFFFALILVIQFSAMLFHRFGTISHILSSTDLNWFCSKKSEDLSQDALLDKNAIAIVKDLQKLNGLDDDYDNDSGSGPHNVGRRKTIHNLEKARQKKRNIGTLDVAFKKRFFNMNANDGPGTPVLNRKMTLRRETLKALETRRNSVMAERRKSQMQTLGANNEYGVTGMLNNNVGPRHRASNANISVKDVFSEPNGGQINRAYEASLGDDDDSNSMRLQPRQNQVSFQGRF

Target ORF information:

RefSeq Version	XM_012694438.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori chitin synthase A (LOC100884166), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012694438.1

ORF Insert Sequence:

ATGGCGACGT CTGGAGGGAA ACGGCGAGAT GAGGGTAGCG ATAACTCTGA CGATGAACTC 
ACACCCCTGG CGAACGAAAT ATATGGTGGA AGTCAGCGCA CAGTACAAGA AACGAAAGGA 
TGGGACGTGT TCAGAGAATT TCCGCCGAAA CAGGACAGTG TGTCCATGGA GACTCAGAAA 
TGTTTGGAAT TCACAGTGCG AATGTTAAAA GTGGTCGCTT ACCTGGTCAC CTTCATCGTG 
GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG ACAATTTTGT TCATGACGTC ACAAATTAGG 
AAAGACAAGC GATTAGAATT CTGCAATAAA AACTTAGGTC GAGATAAGCA GTTTGTGGTG 
AGTTTACCAG ACGAGGAGCG TGTTGCCTGG ATGTGGGCTA TCCTAGCTGC CTTTGCTATT 
CCGGAATTAG GAACGATGAT CAGGTCTATA AGAATATGCT TCTTCAAAAC ATCAAAGAAG 
CCGAGTTTTG TACAATTTTC TGTGGTCTTT ATCGCTGAGT CCTTACATAC CATAGGAATG 
GGTTTACTAT TCTTTAAAAT CCTGCCTGAA TTAGATGTAG TCAAAGGAGC TATGATAACG 
AACTGTCTCT GTATAATTCC CGCGGTGCTC GGTCTGCTGT CAAGAAACTC TCGAGACTCG 
AAACGATTTG TCAAAGTCAT CGTCGATATG GCCGCTATAG TTGCGCAAGT GACCGGTTTT 
ATAGTTTGGC CCCTGTTGGA AAACAAAGCT GTTTTGTGGT TGATCCCGAT CTCTGCTTTA 
TGTATTTCGT TAGGTTGGTG GGAAAACTAT GTGACACGAC AAAGTCCAAT AGGAATAATC 
AAAAGCCTGG GGAGACTAAA AGAGGAATTA AATTTTACTC GCTACTTTAC GTATCGATTC 
ATCTCCGTGT GGAAGATTTT ACTGTTTCTT TTCTGCAATC TATTCTTCAT GTGGTTGGAC 
GGAGATGAAC CGGCAATGTT TTTCCAACTT TTCAATCCTG GATTTGGGCC ACATAGCATT 
GTCGTTGAAG AGGTTCAAAT CCAACTCGGG GGAACGGTTA TTCCAGATTT GGCAAACATA 
ACCCTTACAG GAGACTCCGT CGAAATCGCG GCTGTACATA AGTCCGCCGT CTACGTCATG 
ATGATCCAAA TATTTGCAGC CTACATCTGC TACATCTTTG GGAAATTTGC TTGCAAAATT 
GTTATTCAAG GGTTCAGTTA CGCTTTTCCG ATAAACTTGG TCATACCTTT GGTGGTGAAC 
TTTTTGATTG CTGCTTGTGG TATCAGAAAT GGCGACACCT GTTTCTTCCA CGGAACGATA 
CCGGACTACC TATTCTTCGA AAGCCCTCCA GTGTTTACGT TGAGCGATTT CATCTCTCGA 
CAAATGGCAT GGGTTTGGTT GTTATGGCTG CTATCACAAA CCTGGATCAC TATTCATATT 
TGGACCCCCA AAGCAGAACG TTTAGCTTCC ACTGAGAAAT TGTTTGTGTT GCCTATGTAC 
AATGGTCTAC TCATAGACCA AAGTATGGCA CTGAATAGGA AAAGAGATGA CCATAAGGAT 
GTCAAAACAG AAGATCTTGC CGAAATCGAA AAGGAAAAAG GTGATGAATA CTATGAAACA 
ATATCAGTAC ATACAGACAA TACTGGATCT TCCCCAAAGA CTGTGAAATC ATCAGACCAG 
ATCACGAGGA TTTATGCTTG TGCTACTATG TGGCACGAGA CCAAAGATGA GATGATGGAG 
TTCTTGAAAT CTATTCTTCG TCTCGATGAA GATCAGTGTG CTCGCCGCGT CGCTCAGAAG 
TATCTTCGTG TAGTAGATCC TGACTATTAC GAATTCGAAA CTCACATTTT CTTGGACGAC 
GCTTTCGAAA TTTCGGATCA TAGTGACGAC GAATCTCAAG TAAACCGGTT TGTCAAGCTT 
CTGGTAGATA CCATCGACGA AGCTGCTTCT AATGTACATC AAACGAACAT TCGTATACGT 
CCACCGAAGA GATATCCTGC GCCATACGGA GGTAGACTGA CATGGGTGTT ACCAGGAAAG 
ACTAAAATGA TCTGTCACTT GAAGGATAAG GCAAAGATTC GACACAGGAA ACGTTGGTCT 
CAGGTGATGT ACATGTACTA CCTTCTCGGT CATCGTCTAA TGGAATTGGC TATTTCGGTT 
GATCGTAAAG AAGTTATGGC TGAGAATACT TACCTGCTTA CACTCGATGG AGATATCGAT 
TTCCAACCTC ACGCTGTACG ATTACTCATT GATTTAATGA AGAAAAACAA GAACCTCGGA 
GCTGCCTGCG GGCGTATTCA CCCTGTTGGA TCTGGACCTA TGGTGTGGTA TCAATTATTC 
GAGTATGCTA TCGGACATTG GCTGCAAAAG GCCACCGAAC ACATGATCGG CTGCGTGCTC 
TGTAGTCCCG GATGCTTCTC GCTGTTCAGA GGAAAGGCTC TGATGGACGA CAACGTTATG 
AAGAAATATA CTTTGAAATC TGACGAAGCC CGTCACTACG TACAATACGA TCAGGGAGAA 
GATCGATGGC TATGCACTCT GCTACTTCAA CGTGGCTATC GTGTAGAATA TTCAGCTGCA 
TCGGACGCCT ACACCCATTG CCCTGAAGGT TTTAGCGAGT TCTACAATCA ACGACGTAGA 
TGGGTGCCTT CGACCATTGC TAACATTATG GACTTGCTTG TGGATTATAA ACATACCATT 
AAAATCAATG ACAATATTTC CTCACCGTAC ATCGCATACC AGATGATGTT GATGGGCGGT 
ACGATTCTGG GACCTGGAAC TATATTTCTT ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT 
AGAATTGATA ATTGGACATC CTTCGAATAC AATCTATATC CTATAATGTT GTTTATGTTT 
GTTTGTTTCA CAATGAAGTC AGAATATCAA TTATTGGTCG CGCAAATATT ATCAACGGCC 
TATGCTATGA TAATGATGGC TGTAATTGTG GGTACAGCAC TGCAATTAGG CGAGGACGGT 
ATCGGATCCC CATCCGCTAT ATTCTTGATT TCACTGTCAA GTTCTTTCTT TATAGCGGCC 
TGTTTACATC CCCAGGAGTT TTGGTGTATC GTTCCCGGTA TCATTTATCT ATTATCTATA 
CCCTCTATGT ACTTGCTTTT GATTTTGTAT TCTATTATAA ATCTGAATGT AGTATCGTGG 
GGTACGCGAG AAGTGCAAAC CAAGAAAACA AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT 
GCCGAAGAGG CGAAAAAGAA GGCGAAACAA AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC 
AATTCTAACG AAGAGGAAGG CTCCATAGAA CTATCGTTTG CTGGTCTTTT TAAATGTTTA 
CTGTGTACTC ATCCTAAAGG AAACGAAGAA AAAGTACAGC TCATGCATAT CGCTTCAACA 
CTTGAGAAAT TGGAAAAGAA AATTGAAAAC GTCGAGAAGG CCGTGGATCC GCACGGTCTA 
TCCAGAAGTC GCAAACTATC GCTAGGCCCT CGTGGTAGCA CTAACGGAGA CCACCAGTTG 
GACGCTCTTA ATGAGGACCC CGAAGAAGAA AACGATTCCG ATTCCGACAC CGGCACTCTA 
TCCACTGAAC CAAGGGAACG ACGAGATGAT CTTATAAATC CATATTGGAT TGAGGATCCC 
GGCTTGAAAA AAGGCGAAGT AGATTTCTTG AGCCCGCCCG AGGTACAATT CTGGAAAGAC 
CTTATTGACA AGTATTTATA TCCTATTGAC GAAGACAAGG ACGAGAAGGC ACGTATTTCG 
AGAGACTTAA AAGAACTGAG AGATTCTTCT GTATTTTCAT TCTTTATGAT CAATGCTCTT 
TTTGTGCTCA TTGTATTTTT GCTACAATTG AACAAGGACA ACCTTCACAT TAAATGGCCC 
TTCGGAGTTA AGACTAACAT TACCTATGAT GAGGTCTCAC AAGAGCTTCT CATCTCGAAG 
GAATACTTGC AATTAGAGCC TATCGGTCTG GTATTTGTGT TCTTCTTTGC CTTGATTTTG 
GTCATACAAT TTTCGGCCAT GTTGTTCCAT CGATTCGGAA CTATTTCGCA TATTCTATCA 
TCGACGGATC TCAACTGGTT CTGTTCAAAG AAATCGGAAG ATTTGTCACA GGATGCGCTT 
TTGGATAAGA ATGCAATAGC AATAGTGAAA GACCTTCAGA AATTAAACGG TCTGGACGAT 
GATTATGATA ACGATTCAGG ATCTGGACCA CACAACGTCG GCCGAAGGAA GACAATACAC 
AATTTAGAGA AAGCTAGACA GAAGAAACGT AACATAGGAA CACTAGATGT GGCTTTCAAG 
AAACGATTCT TCAATATGAA TGCTAATGAT GGTCCGGGTA CCCCAGTTTT AAATCGTAAA 
ATGACATTGA GAAGGGAGAC ATTGAAAGCT TTAGAGACCC GTCGTAACTC TGTAATGGCA 
GAAAGAAGGA AGTCGCAAAT GCAGACACTT GGCGCTAATA ATGAATACGG TGTTACTGGA 
ATGCTCAATA ATAACGTTGG ACCTCGTCAT AGAGCATCGA ATGCTAATAT TTCAGTGAAG 
GATGTATTCT CGGAGCCCAA CGGAGGACAG ATCAACAGGG CCTACGAAGC CTCACTCGGG 
GACGACGATG ACTCAAATTC CATGCGACTT CAGCCAAGAC AAAACCAAGT TTCATTCCAA 
GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100884166 ( XM_012694439.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_012549893.1 Bombyx mori chitin synthase A (LOC100884166), transcript variant X3, mRNA.

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CloneID	OBg14618
Clone ID Related Accession (Same CDS sequence)	XM_012694439.1
Accession Version	XM_012694439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4692bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433347200000
Organism	Bombyx mori(Domestic silkworm)
Product	chitin synthase A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGT CTGGAGGGAA ACGGCGAGAT GAGGGTAGCG ATAACTCTGA CGATGAACTC 
ACACCCCTGG CGAACGAAAT ATATGGTGGA AGTCAGCGCA CAGTACAAGA AACGAAAGGA 
TGGGACGTGT TCAGAGAATT TCCGCCGAAA CAGGACAGTG TGTCCATGGA GACTCAGAAA 
TGTTTGGAAT TCACAGTGCG AATGTTAAAA GTGGTCGCTT ACCTGGTCAC CTTCATCGTG 
GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG ACAATTTTGT TCATGACGTC ACAAATTAGG 
AAAGACAAGC GATTAGAATT CTGCAATAAA AACTTAGGTC GAGATAAGCA GTTTGTGGTG 
AGTTTACCAG ACGAGGAGCG TGTTGCCTGG ATGTGGGCTA TCCTAGCTGC CTTTGCTATT 
CCGGAATTAG GAACGATGAT CAGGTCTATA AGAATATGCT TCTTCAAAAC ATCAAAGAAG 
CCGAGTTTTG TACAATTTTC TGTGGTCTTT ATCGCTGAGT CCTTACATAC CATAGGAATG 
GGTTTACTAT TCTTTAAAAT CCTGCCTGAA TTAGATGTAG TCAAAGGAGC TATGATAACG 
AACTGTCTCT GTATAATTCC CGCGGTGCTC GGTCTGCTGT CAAGAAACTC TCGAGACTCG 
AAACGATTTG TCAAAGTCAT CGTCGATATG GCCGCTATAG TTGCGCAAGT GACCGGTTTT 
ATAGTTTGGC CCCTGTTGGA AAACAAAGCT GTTTTGTGGT TGATCCCGAT CTCTGCTTTA 
TGTATTTCGT TAGGTTGGTG GGAAAACTAT GTGACACGAC AAAGTCCAAT AGGAATAATC 
AAAAGCCTGG GGAGACTAAA AGAGGAATTA AATTTTACTC GCTACTTTAC GTATCGATTC 
ATCTCCGTGT GGAAGATTTT ACTGTTTCTT TTCTGCAATC TATTCTTCAT GTGGTTGGAC 
GGAGATGAAC CGGCAATGTT TTTCCAACTT TTCAATCCTG GATTTGGGCC ACATAGCATT 
GTCGTTGAAG AGGTTCAAAT CCAACTCGGG GGAACGGTTA TTCCAGATTT GGCAAACATA 
ACCCTTACAG GAGACTCCGT CGAAATCGCG GCTGTACATA AGTCCGCCGT CTACGTCATG 
ATGATCCAAA TATTTGCAGC CTACATCTGC TACATCTTTG GGAAATTTGC TTGCAAAATT 
GTTATTCAAG GGTTCAGTTA CGCTTTTCCG ATAAACTTGG TCATACCTTT GGTGGTGAAC 
TTTTTGATTG CTGCTTGTGG TATCAGAAAT GGCGACACCT GTTTCTTCCA CGGAACGATA 
CCGGACTACC TATTCTTCGA AAGCCCTCCA GTGTTTACGT TGAGCGATTT CATCTCTCGA 
CAAATGGCAT GGGTTTGGTT GTTATGGCTG CTATCACAAA CCTGGATCAC TATTCATATT 
TGGACCCCCA AAGCAGAACG TTTAGCTTCC ACTGAGAAAT TGTTTGTGTT GCCTATGTAC 
AATGGTCTAC TCATAGACCA AAGTATGGCA CTGAATAGGA AAAGAGATGA CCATAAGGAT 
GTCAAAACAG AAGATCTTGC CGAAATCGAA AAGGAAAAAG GTGATGAATA CTATGAAACA 
ATATCAGTAC ATACAGACAA TACTGGATCT TCCCCAAAGA CTGTGAAATC ATCAGACCAG 
ATCACGAGGA TTTATGCTTG TGCTACTATG TGGCACGAGA CCAAAGATGA GATGATGGAG 
TTCTTGAAAT CTATTCTTCG TCTCGATGAA GATCAGTGTG CTCGCCGCGT CGCTCAGAAG 
TATCTTCGTG TAGTAGATCC TGACTATTAC GAATTCGAAA CTCACATTTT CTTGGACGAC 
GCTTTCGAAA TTTCGGATCA TAGTGACGAC GAATCTCAAG TAAACCGGTT TGTCAAGCTT 
CTGGTAGATA CCATCGACGA AGCTGCTTCT AATGTACATC AAACGAACAT TCGTATACGT 
CCACCGAAGA GATATCCTGC GCCATACGGA GGTAGACTGA CATGGGTGTT ACCAGGAAAG 
ACTAAAATGA TCTGTCACTT GAAGGATAAG GCAAAGATTC GACACAGGAA ACGTTGGTCT 
CAGGTGATGT ACATGTACTA CCTTCTCGGT CATCGTCTAA TGGAATTGGC TATTTCGGTT 
GATCGTAAAG AAGTTATGGC TGAGAATACT TACCTGCTTA CACTCGATGG AGATATCGAT 
TTCCAACCTC ACGCTGTACG ATTACTCATT GATTTAATGA AGAAAAACAA GAACCTCGGA 
GCTGCCTGCG GGCGTATTCA CCCTGTTGGA TCTGGACCTA TGGTGTGGTA TCAATTATTC 
GAGTATGCTA TCGGACATTG GCTGCAAAAG GCCACCGAAC ACATGATCGG CTGCGTGCTC 
TGTAGTCCCG GATGCTTCTC GCTGTTCAGA GGAAAGGCTC TGATGGACGA CAACGTTATG 
AAGAAATATA CTTTGAAATC TGACGAAGCC CGTCACTACG TACAATACGA TCAGGGAGAA 
GATCGATGGC TATGCACTCT GCTACTTCAA CGTGGCTATC GTGTAGAATA TTCAGCTGCA 
TCGGACGCCT ACACCCATTG CCCTGAAGGT TTTAGCGAGT TCTACAATCA ACGACGTAGA 
TGGGTGCCTT CGACCATTGC TAACATTATG GACTTGCTTG TGGATTATAA ACATACCATT 
AAAATCAATG ACAATATTTC CTCACCGTAC ATCGCATACC AGATGATGTT GATGGGCGGT 
ACGATTCTGG GACCTGGAAC TATATTTCTT ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT 
AGAATTGATA ATTGGACATC CTTCGAATAC AATCTATATC CTATAATGTT GTTTATGTTT 
GTTTGTTTCA CAATGAAGTC AGAATATCAA TTATTGGTCG CGCAAATATT ATCAACGGCC 
TATGCTATGA TAATGATGGC TGTAATTGTG GGTACAGCAC TGCAATTAGG CGAGGACGGT 
ATCGGATCCC CATCCGCTAT ATTCTTGATT TCACTGTCAA GTTCTTTCTT TATAGCGGCC 
TGTTTACATC CCCAGGAGTT TTGGTGTATC GTTCCCGGTA TCATTTATCT ATTATCTATA 
CCCTCTATGT ACTTGCTTTT GATTTTGTAT TCTATTATAA ATCTGAATGT AGTATCGTGG 
GGTACGCGAG AAGTGCAAAC CAAGAAAACA AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT 
GCCGAAGAGG CGAAAAAGAA GGCGAAACAA AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC 
AATTCTAACG AAGAGGAAGG CTCCATAGAA CTATCGTTTG CTGGTCTTTT TAAATGTTTA 
CTGTGTACTC ATCCTAAAGG AAACGAAGAA AAAGTACAGC TCATGCATAT CGCTTCAACA 
CTTGAGAAAT TGGAAAAGAA AATTGAAAAC GTCGAGAAGG CCGTGGATCC GCACGGTCTA 
TCCAGAAGTC GCAAACTATC GCTAGGCCCT CGTGGTAGCA CTAACGGAGA CCACCAGTTG 
GACGCTCTTA ATGAGGACCC CGAAGAAGAA AACGATTCCG ATTCCGACAC CGGCACTCTA 
TCCACTGAAC CAAGGGAACG ACGAGATGAT CTTATAAATC CATATTGGAT TGAGGATCCC 
GGCTTGAAAA AAGGCGAAGT AGATTTCTTG AGCCCGCCCG AGGTACAATT CTGGAAAGAC 
CTTATTGACA AGTATTTATA TCCTATTGAC GAAGACAAGG ACGAGAAGGC TCGCATAGCA 
GGCGATCTAC TAGAACTTCG CAATAAATCA GTTTTCGCAT TTGTTATGTT CAATGCTTTA 
TTTATATTGA TAGTATTTTT ATTACAACTC AATAAAGATC AGCTTCACGT GGTCTGGCCT 
TTGGGAGTTA AGACAAACAT TACGTACATG GAGGAAACAG GCCAGCTTCT CATCTCGAAG 
GAATACTTGC AATTAGAGCC TATCGGTCTG GTATTTGTGT TCTTCTTTGC CTTGATTTTG 
GTCATACAAT TTTCGGCCAT GTTGTTCCAT CGATTCGGAA CTATTTCGCA TATTCTATCA 
TCGACGGATC TCAACTGGTT CTGTTCAAAG AAATCGGAAG ATTTGTCACA GGATGCGCTT 
TTGGATAAGA ATGCAATAGC AATAGTGAAA GACCTTCAGA AATTAAACGG TCTGGACGAT 
GATTATGATA ACGATTCAGG ATCTGGACCA CACAACGTCG GCCGAAGGAA GACAATACAC 
AATTTAGAGA AAGCTAGACA GAAGAAACGT AACATAGGAA CACTAGATGT GGCTTTCAAG 
AAACGATTCT TCAATATGAA TGCTAATGAT GGTCCGGGTA CCCCAGTTTT AAATCGTAAA 
ATGACATTGA GAAGGGAGAC ATTGAAAGCT TTAGAGACCC GTCGTAACTC TGTAATGGCA 
GAAAGAAGGA AGTCGCAAAT GCAGACACTT GGCGCTAATA ATGAATACGG TGTTACTGGA 
ATGCTCAATA ATAACGTTGG ACCTCGTCAT AGAGCATCGA ATGCTAATAT TTCAGTGAAG 
GATGTATTCT CGGAGCCCAA CGGAGGACAG ATCAACAGGG CCTACGAAGC CTCACTCGGG 
GACGACGATG ACTCAAATTC CATGCGACTT CAGCCAAGAC AAAACCAAGT TTCATTCCAA 
GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012549893.1
CDS	154..4845
Translation	MATSGGKRRDEGSDNSDDELTPLANEIYGGSQRTVQETKGWDVFREFPPKQDSVSMETQKCLEFTVRMLKVVAYLVTFIVVLGSGVIAKGTILFMTSQIRKDKRLEFCNKNLGRDKQFVVSLPDEERVAWMWAILAAFAIPELGTMIRSIRICFFKTSKKPSFVQFSVVFIAESLHTIGMGLLFFKILPELDVVKGAMITNCLCIIPAVLGLLSRNSRDSKRFVKVIVDMAAIVAQVTGFIVWPLLENKAVLWLIPISALCISLGWWENYVTRQSPIGIIKSLGRLKEELNFTRYFTYRFISVWKILLFLFCNLFFMWLDGDEPAMFFQLFNPGFGPHSIVVEEVQIQLGGTVIPDLANITLTGDSVEIAAVHKSAVYVMMIQIFAAYICYIFGKFACKIVIQGFSYAFPINLVIPLVVNFLIAACGIRNGDTCFFHGTIPDYLFFESPPVFTLSDFISRQMAWVWLLWLLSQTWITIHIWTPKAERLASTEKLFVLPMYNGLLIDQSMALNRKRDDHKDVKTEDLAEIEKEKGDEYYETISVHTDNTGSSPKTVKSSDQITRIYACATMWHETKDEMMEFLKSILRLDEDQCARRVAQKYLRVVDPDYYEFETHIFLDDAFEISDHSDDESQVNRFVKLLVDTIDEAASNVHQTNIRIRPPKRYPAPYGGRLTWVLPGKTKMICHLKDKAKIRHRKRWSQVMYMYYLLGHRLMELAISVDRKEVMAENTYLLTLDGDIDFQPHAVRLLIDLMKKNKNLGAACGRIHPVGSGPMVWYQLFEYAIGHWLQKATEHMIGCVLCSPGCFSLFRGKALMDDNVMKKYTLKSDEARHYVQYDQGEDRWLCTLLLQRGYRVEYSAASDAYTHCPEGFSEFYNQRRRWVPSTIANIMDLLVDYKHTIKINDNISSPYIAYQMMLMGGTILGPGTIFLMLVGAFVAAFRIDNWTSFEYNLYPIMLFMFVCFTMKSEYQLLVAQILSTAYAMIMMAVIVGTALQLGEDGIGSPSAIFLISLSSSFFIAACLHPQEFWCIVPGIIYLLSIPSMYLLLILYSIINLNVVSWGTREVQTKKTKKEIEQEKKDAEEAKKKAKQKSLLGFLQGVNSNEEEGSIELSFAGLFKCLLCTHPKGNEEKVQLMHIASTLEKLEKKIENVEKAVDPHGLSRSRKLSLGPRGSTNGDHQLDALNEDPEEENDSDSDTGTLSTEPRERRDDLINPYWIEDPGLKKGEVDFLSPPEVQFWKDLIDKYLYPIDEDKDEKARIAGDLLELRNKSVFAFVMFNALFILIVFLLQLNKDQLHVVWPLGVKTNITYMEETGQLLISKEYLQLEPIGLVFVFFFALILVIQFSAMLFHRFGTISHILSSTDLNWFCSKKSEDLSQDALLDKNAIAIVKDLQKLNGLDDDYDNDSGSGPHNVGRRKTIHNLEKARQKKRNIGTLDVAFKKRFFNMNANDGPGTPVLNRKMTLRRETLKALETRRNSVMAERRKSQMQTLGANNEYGVTGMLNNNVGPRHRASNANISVKDVFSEPNGGQINRAYEASLGDDDDSNSMRLQPRQNQVSFQGRF

Target ORF information:

RefSeq Version	XM_012694439.1
Organism	Bombyx mori(Domestic silkworm)
Definition	Bombyx mori chitin synthase A (LOC100884166), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012694439.1

ORF Insert Sequence:

ATGGCGACGT CTGGAGGGAA ACGGCGAGAT GAGGGTAGCG ATAACTCTGA CGATGAACTC 
ACACCCCTGG CGAACGAAAT ATATGGTGGA AGTCAGCGCA CAGTACAAGA AACGAAAGGA 
TGGGACGTGT TCAGAGAATT TCCGCCGAAA CAGGACAGTG TGTCCATGGA GACTCAGAAA 
TGTTTGGAAT TCACAGTGCG AATGTTAAAA GTGGTCGCTT ACCTGGTCAC CTTCATCGTG 
GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG ACAATTTTGT TCATGACGTC ACAAATTAGG 
AAAGACAAGC GATTAGAATT CTGCAATAAA AACTTAGGTC GAGATAAGCA GTTTGTGGTG 
AGTTTACCAG ACGAGGAGCG TGTTGCCTGG ATGTGGGCTA TCCTAGCTGC CTTTGCTATT 
CCGGAATTAG GAACGATGAT CAGGTCTATA AGAATATGCT TCTTCAAAAC ATCAAAGAAG 
CCGAGTTTTG TACAATTTTC TGTGGTCTTT ATCGCTGAGT CCTTACATAC CATAGGAATG 
GGTTTACTAT TCTTTAAAAT CCTGCCTGAA TTAGATGTAG TCAAAGGAGC TATGATAACG 
AACTGTCTCT GTATAATTCC CGCGGTGCTC GGTCTGCTGT CAAGAAACTC TCGAGACTCG 
AAACGATTTG TCAAAGTCAT CGTCGATATG GCCGCTATAG TTGCGCAAGT GACCGGTTTT 
ATAGTTTGGC CCCTGTTGGA AAACAAAGCT GTTTTGTGGT TGATCCCGAT CTCTGCTTTA 
TGTATTTCGT TAGGTTGGTG GGAAAACTAT GTGACACGAC AAAGTCCAAT AGGAATAATC 
AAAAGCCTGG GGAGACTAAA AGAGGAATTA AATTTTACTC GCTACTTTAC GTATCGATTC 
ATCTCCGTGT GGAAGATTTT ACTGTTTCTT TTCTGCAATC TATTCTTCAT GTGGTTGGAC 
GGAGATGAAC CGGCAATGTT TTTCCAACTT TTCAATCCTG GATTTGGGCC ACATAGCATT 
GTCGTTGAAG AGGTTCAAAT CCAACTCGGG GGAACGGTTA TTCCAGATTT GGCAAACATA 
ACCCTTACAG GAGACTCCGT CGAAATCGCG GCTGTACATA AGTCCGCCGT CTACGTCATG 
ATGATCCAAA TATTTGCAGC CTACATCTGC TACATCTTTG GGAAATTTGC TTGCAAAATT 
GTTATTCAAG GGTTCAGTTA CGCTTTTCCG ATAAACTTGG TCATACCTTT GGTGGTGAAC 
TTTTTGATTG CTGCTTGTGG TATCAGAAAT GGCGACACCT GTTTCTTCCA CGGAACGATA 
CCGGACTACC TATTCTTCGA AAGCCCTCCA GTGTTTACGT TGAGCGATTT CATCTCTCGA 
CAAATGGCAT GGGTTTGGTT GTTATGGCTG CTATCACAAA CCTGGATCAC TATTCATATT 
TGGACCCCCA AAGCAGAACG TTTAGCTTCC ACTGAGAAAT TGTTTGTGTT GCCTATGTAC 
AATGGTCTAC TCATAGACCA AAGTATGGCA CTGAATAGGA AAAGAGATGA CCATAAGGAT 
GTCAAAACAG AAGATCTTGC CGAAATCGAA AAGGAAAAAG GTGATGAATA CTATGAAACA 
ATATCAGTAC ATACAGACAA TACTGGATCT TCCCCAAAGA CTGTGAAATC ATCAGACCAG 
ATCACGAGGA TTTATGCTTG TGCTACTATG TGGCACGAGA CCAAAGATGA GATGATGGAG 
TTCTTGAAAT CTATTCTTCG TCTCGATGAA GATCAGTGTG CTCGCCGCGT CGCTCAGAAG 
TATCTTCGTG TAGTAGATCC TGACTATTAC GAATTCGAAA CTCACATTTT CTTGGACGAC 
GCTTTCGAAA TTTCGGATCA TAGTGACGAC GAATCTCAAG TAAACCGGTT TGTCAAGCTT 
CTGGTAGATA CCATCGACGA AGCTGCTTCT AATGTACATC AAACGAACAT TCGTATACGT 
CCACCGAAGA GATATCCTGC GCCATACGGA GGTAGACTGA CATGGGTGTT ACCAGGAAAG 
ACTAAAATGA TCTGTCACTT GAAGGATAAG GCAAAGATTC GACACAGGAA ACGTTGGTCT 
CAGGTGATGT ACATGTACTA CCTTCTCGGT CATCGTCTAA TGGAATTGGC TATTTCGGTT 
GATCGTAAAG AAGTTATGGC TGAGAATACT TACCTGCTTA CACTCGATGG AGATATCGAT 
TTCCAACCTC ACGCTGTACG ATTACTCATT GATTTAATGA AGAAAAACAA GAACCTCGGA 
GCTGCCTGCG GGCGTATTCA CCCTGTTGGA TCTGGACCTA TGGTGTGGTA TCAATTATTC 
GAGTATGCTA TCGGACATTG GCTGCAAAAG GCCACCGAAC ACATGATCGG CTGCGTGCTC 
TGTAGTCCCG GATGCTTCTC GCTGTTCAGA GGAAAGGCTC TGATGGACGA CAACGTTATG 
AAGAAATATA CTTTGAAATC TGACGAAGCC CGTCACTACG TACAATACGA TCAGGGAGAA 
GATCGATGGC TATGCACTCT GCTACTTCAA CGTGGCTATC GTGTAGAATA TTCAGCTGCA 
TCGGACGCCT ACACCCATTG CCCTGAAGGT TTTAGCGAGT TCTACAATCA ACGACGTAGA 
TGGGTGCCTT CGACCATTGC TAACATTATG GACTTGCTTG TGGATTATAA ACATACCATT 
AAAATCAATG ACAATATTTC CTCACCGTAC ATCGCATACC AGATGATGTT GATGGGCGGT 
ACGATTCTGG GACCTGGAAC TATATTTCTT ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT 
AGAATTGATA ATTGGACATC CTTCGAATAC AATCTATATC CTATAATGTT GTTTATGTTT 
GTTTGTTTCA CAATGAAGTC AGAATATCAA TTATTGGTCG CGCAAATATT ATCAACGGCC 
TATGCTATGA TAATGATGGC TGTAATTGTG GGTACAGCAC TGCAATTAGG CGAGGACGGT 
ATCGGATCCC CATCCGCTAT ATTCTTGATT TCACTGTCAA GTTCTTTCTT TATAGCGGCC 
TGTTTACATC CCCAGGAGTT TTGGTGTATC GTTCCCGGTA TCATTTATCT ATTATCTATA 
CCCTCTATGT ACTTGCTTTT GATTTTGTAT TCTATTATAA ATCTGAATGT AGTATCGTGG 
GGTACGCGAG AAGTGCAAAC CAAGAAAACA AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT 
GCCGAAGAGG CGAAAAAGAA GGCGAAACAA AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC 
AATTCTAACG AAGAGGAAGG CTCCATAGAA CTATCGTTTG CTGGTCTTTT TAAATGTTTA 
CTGTGTACTC ATCCTAAAGG AAACGAAGAA AAAGTACAGC TCATGCATAT CGCTTCAACA 
CTTGAGAAAT TGGAAAAGAA AATTGAAAAC GTCGAGAAGG CCGTGGATCC GCACGGTCTA 
TCCAGAAGTC GCAAACTATC GCTAGGCCCT CGTGGTAGCA CTAACGGAGA CCACCAGTTG 
GACGCTCTTA ATGAGGACCC CGAAGAAGAA AACGATTCCG ATTCCGACAC CGGCACTCTA 
TCCACTGAAC CAAGGGAACG ACGAGATGAT CTTATAAATC CATATTGGAT TGAGGATCCC 
GGCTTGAAAA AAGGCGAAGT AGATTTCTTG AGCCCGCCCG AGGTACAATT CTGGAAAGAC 
CTTATTGACA AGTATTTATA TCCTATTGAC GAAGACAAGG ACGAGAAGGC TCGCATAGCA 
GGCGATCTAC TAGAACTTCG CAATAAATCA GTTTTCGCAT TTGTTATGTT CAATGCTTTA 
TTTATATTGA TAGTATTTTT ATTACAACTC AATAAAGATC AGCTTCACGT GGTCTGGCCT 
TTGGGAGTTA AGACAAACAT TACGTACATG GAGGAAACAG GCCAGCTTCT CATCTCGAAG 
GAATACTTGC AATTAGAGCC TATCGGTCTG GTATTTGTGT TCTTCTTTGC CTTGATTTTG 
GTCATACAAT TTTCGGCCAT GTTGTTCCAT CGATTCGGAA CTATTTCGCA TATTCTATCA 
TCGACGGATC TCAACTGGTT CTGTTCAAAG AAATCGGAAG ATTTGTCACA GGATGCGCTT 
TTGGATAAGA ATGCAATAGC AATAGTGAAA GACCTTCAGA AATTAAACGG TCTGGACGAT 
GATTATGATA ACGATTCAGG ATCTGGACCA CACAACGTCG GCCGAAGGAA GACAATACAC 
AATTTAGAGA AAGCTAGACA GAAGAAACGT AACATAGGAA CACTAGATGT GGCTTTCAAG 
AAACGATTCT TCAATATGAA TGCTAATGAT GGTCCGGGTA CCCCAGTTTT AAATCGTAAA 
ATGACATTGA GAAGGGAGAC ATTGAAAGCT TTAGAGACCC GTCGTAACTC TGTAATGGCA 
GAAAGAAGGA AGTCGCAAAT GCAGACACTT GGCGCTAATA ATGAATACGG TGTTACTGGA 
ATGCTCAATA ATAACGTTGG ACCTCGTCAT AGAGCATCGA ATGCTAATAT TTCAGTGAAG 
GATGTATTCT CGGAGCCCAA CGGAGGACAG ATCAACAGGG CCTACGAAGC CTCACTCGGG 
GACGACGATG ACTCAAATTC CATGCGACTT CAGCCAAGAC AAAACCAAGT TTCATTCCAA 
GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100884166 ( XM_021351080.1 ) cDNA ORF clone, Bombyx mori(domestic silkworm) -> XP_021206755.1 Bombyx mori chitin synthase A (LOC100884166), transcript variant X1, mRNA.

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CloneID	OBg26982
Clone ID Related Accession (Same CDS sequence)	XM_021351080.1
Accession Version	XM_021351080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1496505600000
Organism	Bombyx mori(domestic silkworm)
Product	chitin synthase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004582020.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombyx mori Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAC AAGAACGTAG GAGTCAATAC GAGAACGAGG AGACGGAGGA AGTCGTTTAC 
ATTCCAAATA ACAATTACTC GATATACAGT AATGGAACTT TGCATTACTG TAGCATATAC 
AGTCAGCGCA CAGTACAAGA AACGAAAGGA TGGGACGTGT TCAGAGAATT TCCGCCGAAA 
CAGGACAGTG TGTCCATGGA GACTCAGAAA TGTTTGGAAT TCACAGTGCG AATGTTAAAA 
GTGGTCGCTT ACCTGGTCAC CTTCATCGTG GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG 
ACAATTTTGT TCATGACGTC ACAAATTAGG AAAGACAAGC GATTAGAATT CTGCAATAAA 
AACTTAGGTC GAGATAAGCA GTTTGTGGTG AGTTTACCAG ACGAGGAGCG TGTTGCCTGG 
ATGTGGGCTA TCCTAGCTGC CTTTGCTATT CCGGAATTAG GAACGATGAT CAGGTCTATA 
AGAATATGCT TCTTCAAAAC ATCAAAGAAG CCGAGTTTTG TACAATTTTC TGTGGTCTTT 
ATCGCTGAGT CCTTACATAC CATAGGAATG GGTTTACTAT TCTTTAAAAT CCTGCCTGAA 
TTAGATGTAG TCAAAGGAGC TATGATAACG AACTGTCTCT GTATAATTCC CGCGGTGCTC 
GGTCTGCTGT CAAGAAACTC TCGAGACTCG AAACGATTTG TCAAAGTCAT CGTCGATATG 
GCCGCTATAG TTGCGCAAGT GACCGGTTTT ATAGTTTGGC CCCTGTTGGA AAACAAAGCT 
GTTTTGTGGT TGATCCCGAT CTCTGCTTTA TGTATTTCGT TAGGTTGGTG GGAAAACTAT 
GTGACACGAC AAAGTCCAAT AGGAATAATC AAAAGCCTGG GGAGACTAAA AGAGGAATTA 
AATTTTACTC GCTACTTTAC GTATCGATTC ATCTCCGTGT GGAAGATTTT ACTGTTTCTT 
TTCTGCAATC TATTCTTCAT GTGGTTGGAC GGAGATGAAC CGGCAATGTT TTTCCAACTT 
TTCAATCCTG GATTTGGGCC ACATAGCATT GTCGTTGAAG AGGTTCAAAT CCAACTCGGG 
GGAACGGTTA TTCCAGATTT GGCAAACATA ACCCTTACAG GAGACTCCGT CGAAATCGCG 
GCTGTACATA AGTCCGCCGT CTACGTCATG ATGATCCAAA TATTTGCAGC CTACATCTGC 
TACATCTTTG GGAAATTTGC TTGCAAAATT GTTATTCAAG GGTTCAGTTA CGCTTTTCCG 
ATAAACTTGG TCATACCTTT GGTGGTGAAC TTTTTGATTG CTGCTTGTGG TATCAGAAAT 
GGCGACACCT GTTTCTTCCA CGGAACGATA CCGGACTACC TATTCTTCGA AAGCCCTCCA 
GTGTTTACGT TGAGCGATTT CATCTCTCGA CAAATGGCAT GGGTTTGGTT GTTATGGCTG 
CTATCACAAA CCTGGATCAC TATTCATATT TGGACCCCCA AAGCAGAACG TTTAGCTTCC 
ACTGAGAAAT TGTTTGTGTT GCCTATGTAC AATGGTCTAC TCATAGACCA AAGTATGGCA 
CTGAATAGGA AAAGAGATGA CCATAAGGAT GTCAAAACAG AAGATCTTGC CGAAATCGAA 
AAGGAAAAAG GTGATGAATA CTATGAAACA ATATCAGTAC ATACAGACAA TACTGGATCT 
TCCCCAAAGA CTGTGAAATC ATCAGACCAG ATCACGAGGA TTTATGCTTG TGCTACTATG 
TGGCACGAGA CCAAAGATGA GATGATGGAG TTCTTGAAAT CTATTCTTCG TCTCGATGAA 
GATCAGTGTG CTCGCCGCGT CGCTCAGAAG TATCTTCGTG TAGTAGATCC TGACTATTAC 
GAATTCGAAA CTCACATTTT CTTGGACGAC GCTTTCGAAA TTTCGGATCA TAGTGACGAC 
GAATCTCAAG TAAACCGGTT TGTCAAGCTT CTGGTAGATA CCATCGACGA AGCTGCTTCT 
AATGTACATC AAACGAACAT TCGTATACGT CCACCGAAGA GATATCCTGC GCCATACGGA 
GGTAGACTGA CATGGGTGTT ACCAGGAAAG ACTAAAATGA TCTGTCACTT GAAGGATAAG 
GCAAAGATTC GACACAGGAA ACGTTGGTCT CAGGTGATGT ACATGTACTA CCTTCTCGGT 
CATCGTCTAA TGGAATTGGC TATTTCGGTT GATCGTAAAG AAGTTATGGC TGAGAATACT 
TACCTGCTTA CACTCGATGG AGATATCGAT TTCCAACCTC ACGCTGTACG ATTACTCATT 
GATTTAATGA AGAAAAACAA GAACCTCGGA GCTGCCTGCG GGCGTATTCA CCCTGTTGGA 
TCTGGACCTA TGGTGTGGTA TCAATTATTC GAGTATGCTA TCGGACATTG GCTGCAAAAG 
GCCACCGAAC ACATGATCGG CTGCGTGCTC TGTAGTCCCG GATGCTTCTC GCTGTTCAGA 
GGAAAGGCTC TGATGGACGA CAACGTTATG AAGAAATATA CTTTGAAATC TGACGAAGCC 
CGTCACTACG TACAATACGA TCAGGGAGAA GATCGATGGC TATGCACTCT GCTACTTCAA 
CGTGGCTATC GTGTAGAATA TTCAGCTGCA TCGGACGCCT ACACCCATTG CCCTGAAGGT 
TTTAGCGAGT TCTACAATCA ACGACGTAGA TGGGTGCCTT CGACCATTGC TAACATTATG 
GACTTGCTTG TGGATTATAA ACATACCATT AAAATCAATG ACAATATTTC CTCACCGTAC 
ATCGCATACC AGATGATGTT GATGGGCGGT ACGATTCTGG GACCTGGAAC TATATTTCTT 
ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT AGAATTGATA ATTGGACATC CTTCGAATAC 
AATCTATATC CTATAATGTT GTTTATGTTT GTTTGTTTCA CAATGAAGTC AGAATATCAA 
TTATTGGTCG CGCAAATATT ATCAACGGCC TATGCTATGA TAATGATGGC TGTAATTGTG 
GGTACAGCAC TGCAATTAGG CGAGGACGGT ATCGGATCCC CATCCGCTAT ATTCTTGATT 
TCACTGTCAA GTTCTTTCTT TATAGCGGCC TGTTTACATC CCCAGGAGTT TTGGTGTATC 
GTTCCCGGTA TCATTTATCT ATTATCTATA CCCTCTATGT ACTTGCTTTT GATTTTGTAT 
TCTATTATAA ATCTGAATGT AGTATCGTGG GGTACGCGAG AAGTGCAAAC CAAGAAAACA 
AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT GCCGAAGAGG CGAAAAAGAA GGCGAAACAA 
AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC AATTCTAACG AAGAGGAAGG CTCCATAGAA 
CTATCGTTTG CTGGTCTTTT TAAATGTTTA CTGTGTACTC ATCCTAAAGG AAACGAAGAA 
AAAGTACAGC TCATGCATAT CGCTTCAACA CTTGAGAAAT TGGAAAAGAA AATTGAAAAC 
GTCGAGAAGG CCGTGGATCC GCACGGTCTA TCCAGAAGTC GCAAACTATC GCTAGGCCCT 
CGTGGTAGCA CTAACGGAGA CCACCAGTTG GACGCTCTTA ATGAGGACCC CGAAGAAGAA 
AACGATTCCG ATTCCGACAC CGGCACTCTA TCCACTGAAC CAAGGGAACG ACGAGATGAT 
CTTATAAATC CATATTGGAT TGAGGATCCC GGCTTGAAAA AAGGCGAAGT AGATTTCTTG 
AGCCCGCCCG AGGTACAATT CTGGAAAGAC CTTATTGACA AGTATTTATA TCCTATTGAC 
GAAGACAAGG ACGAGAAGGC ACGTATTTCG AGAGACTTAA AAGAACTGAG AGATTCTTCT 
GTATTTTCAT TCTTTATGAT CAATGCTCTT TTTGTGCTCA TTGTATTTTT GCTACAATTG 
AACAAGGACA ACCTTCACAT TAAATGGCCC TTCGGAGTTA AGACTAACAT TACCTATGAT 
GAGGTCTCAC AAGAGCTTCT CATCTCGAAG GAATACTTGC AATTAGAGCC TATCGGTCTG 
GTATTTGTGT TCTTCTTTGC CTTGATTTTG GTCATACAAT TTTCGGCCAT GTTGTTCCAT 
CGATTCGGAA CTATTTCGCA TATTCTATCA TCGACGGATC TCAACTGGTT CTGTTCAAAG 
AAATCGGAAG ATTTGTCACA GGATGCGCTT TTGGATAAGA ATGCAATAGC AATAGTGAAA 
GACCTTCAGA AATTAAACGG TCTGGACGAT GATTATGATA ACGATTCAGG ATCTGGACCA 
CACAACGTCG GCCGAAGGAA GACAATACAC AATTTAGAGA AAGCTAGACA GAAGAAACGT 
AACATAGGAA CACTAGATGT GGCTTTCAAG AAACGATTCT TCAATATGAA TGCTAATGAT 
GGTCCGGGTA CCCCAGTTTT AAATCGTAAA ATGACATTGA GAAGGGAGAC ATTGAAAGCT 
TTAGAGACCC GTCGTAACTC TGTAATGGCA GAAAGAAGGA AGTCGCAAAT GCAGACACTT 
GGCGCTAATA ATGAATACGG TGTTACTGGA ATGCTCAATA ATAACGTTGG ACCTCGTCAT 
AGAGCATCGA ATGCTAATAT TTCAGTGAAG GATGTATTCT CGGAGCCCAA CGGAGGACAG 
ATCAACAGGG CCTACGAAGC CTCACTCGGG GACGACGATG ACTCAAATTC CATGCGACTT 
CAGCCAAGAC AAAACCAAGT TTCATTCCAA GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021206755.1
CDS	131..4852
Translation	MSEQERRSQYENEETEEVVYIPNNNYSIYSNGTLHYCSIYSQRTVQETKGWDVFREFPPKQDSVSMETQKCLEFTVRMLKVVAYLVTFIVVLGSGVIAKGTILFMTSQIRKDKRLEFCNKNLGRDKQFVVSLPDEERVAWMWAILAAFAIPELGTMIRSIRICFFKTSKKPSFVQFSVVFIAESLHTIGMGLLFFKILPELDVVKGAMITNCLCIIPAVLGLLSRNSRDSKRFVKVIVDMAAIVAQVTGFIVWPLLENKAVLWLIPISALCISLGWWENYVTRQSPIGIIKSLGRLKEELNFTRYFTYRFISVWKILLFLFCNLFFMWLDGDEPAMFFQLFNPGFGPHSIVVEEVQIQLGGTVIPDLANITLTGDSVEIAAVHKSAVYVMMIQIFAAYICYIFGKFACKIVIQGFSYAFPINLVIPLVVNFLIAACGIRNGDTCFFHGTIPDYLFFESPPVFTLSDFISRQMAWVWLLWLLSQTWITIHIWTPKAERLASTEKLFVLPMYNGLLIDQSMALNRKRDDHKDVKTEDLAEIEKEKGDEYYETISVHTDNTGSSPKTVKSSDQITRIYACATMWHETKDEMMEFLKSILRLDEDQCARRVAQKYLRVVDPDYYEFETHIFLDDAFEISDHSDDESQVNRFVKLLVDTIDEAASNVHQTNIRIRPPKRYPAPYGGRLTWVLPGKTKMICHLKDKAKIRHRKRWSQVMYMYYLLGHRLMELAISVDRKEVMAENTYLLTLDGDIDFQPHAVRLLIDLMKKNKNLGAACGRIHPVGSGPMVWYQLFEYAIGHWLQKATEHMIGCVLCSPGCFSLFRGKALMDDNVMKKYTLKSDEARHYVQYDQGEDRWLCTLLLQRGYRVEYSAASDAYTHCPEGFSEFYNQRRRWVPSTIANIMDLLVDYKHTIKINDNISSPYIAYQMMLMGGTILGPGTIFLMLVGAFVAAFRIDNWTSFEYNLYPIMLFMFVCFTMKSEYQLLVAQILSTAYAMIMMAVIVGTALQLGEDGIGSPSAIFLISLSSSFFIAACLHPQEFWCIVPGIIYLLSIPSMYLLLILYSIINLNVVSWGTREVQTKKTKKEIEQEKKDAEEAKKKAKQKSLLGFLQGVNSNEEEGSIELSFAGLFKCLLCTHPKGNEEKVQLMHIASTLEKLEKKIENVEKAVDPHGLSRSRKLSLGPRGSTNGDHQLDALNEDPEEENDSDSDTGTLSTEPRERRDDLINPYWIEDPGLKKGEVDFLSPPEVQFWKDLIDKYLYPIDEDKDEKARISRDLKELRDSSVFSFFMINALFVLIVFLLQLNKDNLHIKWPFGVKTNITYDEVSQELLISKEYLQLEPIGLVFVFFFALILVIQFSAMLFHRFGTISHILSSTDLNWFCSKKSEDLSQDALLDKNAIAIVKDLQKLNGLDDDYDNDSGSGPHNVGRRKTIHNLEKARQKKRNIGTLDVAFKKRFFNMNANDGPGTPVLNRKMTLRRETLKALETRRNSVMAERRKSQMQTLGANNEYGVTGMLNNNVGPRHRASNANISVKDVFSEPNGGQINRAYEASLGDDDDSNSMRLQPRQNQVSFQGRF

Target ORF information:

RefSeq Version	XM_021351080.1
Organism	Bombyx mori(domestic silkworm)
Definition	Bombyx mori chitin synthase A (LOC100884166), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021351080.1

ORF Insert Sequence:

ATGAGTGAAC AAGAACGTAG GAGTCAATAC GAGAACGAGG AGACGGAGGA AGTCGTTTAC 
ATTCCAAATA ACAATTACTC GATATACAGT AATGGAACTT TGCATTACTG TAGCATATAC 
AGTCAGCGCA CAGTACAAGA AACGAAAGGA TGGGACGTGT TCAGAGAATT TCCGCCGAAA 
CAGGACAGTG TGTCCATGGA GACTCAGAAA TGTTTGGAAT TCACAGTGCG AATGTTAAAA 
GTGGTCGCTT ACCTGGTCAC CTTCATCGTG GTCCTTGGAT CAGGAGTTAT CGCAAAGGGG 
ACAATTTTGT TCATGACGTC ACAAATTAGG AAAGACAAGC GATTAGAATT CTGCAATAAA 
AACTTAGGTC GAGATAAGCA GTTTGTGGTG AGTTTACCAG ACGAGGAGCG TGTTGCCTGG 
ATGTGGGCTA TCCTAGCTGC CTTTGCTATT CCGGAATTAG GAACGATGAT CAGGTCTATA 
AGAATATGCT TCTTCAAAAC ATCAAAGAAG CCGAGTTTTG TACAATTTTC TGTGGTCTTT 
ATCGCTGAGT CCTTACATAC CATAGGAATG GGTTTACTAT TCTTTAAAAT CCTGCCTGAA 
TTAGATGTAG TCAAAGGAGC TATGATAACG AACTGTCTCT GTATAATTCC CGCGGTGCTC 
GGTCTGCTGT CAAGAAACTC TCGAGACTCG AAACGATTTG TCAAAGTCAT CGTCGATATG 
GCCGCTATAG TTGCGCAAGT GACCGGTTTT ATAGTTTGGC CCCTGTTGGA AAACAAAGCT 
GTTTTGTGGT TGATCCCGAT CTCTGCTTTA TGTATTTCGT TAGGTTGGTG GGAAAACTAT 
GTGACACGAC AAAGTCCAAT AGGAATAATC AAAAGCCTGG GGAGACTAAA AGAGGAATTA 
AATTTTACTC GCTACTTTAC GTATCGATTC ATCTCCGTGT GGAAGATTTT ACTGTTTCTT 
TTCTGCAATC TATTCTTCAT GTGGTTGGAC GGAGATGAAC CGGCAATGTT TTTCCAACTT 
TTCAATCCTG GATTTGGGCC ACATAGCATT GTCGTTGAAG AGGTTCAAAT CCAACTCGGG 
GGAACGGTTA TTCCAGATTT GGCAAACATA ACCCTTACAG GAGACTCCGT CGAAATCGCG 
GCTGTACATA AGTCCGCCGT CTACGTCATG ATGATCCAAA TATTTGCAGC CTACATCTGC 
TACATCTTTG GGAAATTTGC TTGCAAAATT GTTATTCAAG GGTTCAGTTA CGCTTTTCCG 
ATAAACTTGG TCATACCTTT GGTGGTGAAC TTTTTGATTG CTGCTTGTGG TATCAGAAAT 
GGCGACACCT GTTTCTTCCA CGGAACGATA CCGGACTACC TATTCTTCGA AAGCCCTCCA 
GTGTTTACGT TGAGCGATTT CATCTCTCGA CAAATGGCAT GGGTTTGGTT GTTATGGCTG 
CTATCACAAA CCTGGATCAC TATTCATATT TGGACCCCCA AAGCAGAACG TTTAGCTTCC 
ACTGAGAAAT TGTTTGTGTT GCCTATGTAC AATGGTCTAC TCATAGACCA AAGTATGGCA 
CTGAATAGGA AAAGAGATGA CCATAAGGAT GTCAAAACAG AAGATCTTGC CGAAATCGAA 
AAGGAAAAAG GTGATGAATA CTATGAAACA ATATCAGTAC ATACAGACAA TACTGGATCT 
TCCCCAAAGA CTGTGAAATC ATCAGACCAG ATCACGAGGA TTTATGCTTG TGCTACTATG 
TGGCACGAGA CCAAAGATGA GATGATGGAG TTCTTGAAAT CTATTCTTCG TCTCGATGAA 
GATCAGTGTG CTCGCCGCGT CGCTCAGAAG TATCTTCGTG TAGTAGATCC TGACTATTAC 
GAATTCGAAA CTCACATTTT CTTGGACGAC GCTTTCGAAA TTTCGGATCA TAGTGACGAC 
GAATCTCAAG TAAACCGGTT TGTCAAGCTT CTGGTAGATA CCATCGACGA AGCTGCTTCT 
AATGTACATC AAACGAACAT TCGTATACGT CCACCGAAGA GATATCCTGC GCCATACGGA 
GGTAGACTGA CATGGGTGTT ACCAGGAAAG ACTAAAATGA TCTGTCACTT GAAGGATAAG 
GCAAAGATTC GACACAGGAA ACGTTGGTCT CAGGTGATGT ACATGTACTA CCTTCTCGGT 
CATCGTCTAA TGGAATTGGC TATTTCGGTT GATCGTAAAG AAGTTATGGC TGAGAATACT 
TACCTGCTTA CACTCGATGG AGATATCGAT TTCCAACCTC ACGCTGTACG ATTACTCATT 
GATTTAATGA AGAAAAACAA GAACCTCGGA GCTGCCTGCG GGCGTATTCA CCCTGTTGGA 
TCTGGACCTA TGGTGTGGTA TCAATTATTC GAGTATGCTA TCGGACATTG GCTGCAAAAG 
GCCACCGAAC ACATGATCGG CTGCGTGCTC TGTAGTCCCG GATGCTTCTC GCTGTTCAGA 
GGAAAGGCTC TGATGGACGA CAACGTTATG AAGAAATATA CTTTGAAATC TGACGAAGCC 
CGTCACTACG TACAATACGA TCAGGGAGAA GATCGATGGC TATGCACTCT GCTACTTCAA 
CGTGGCTATC GTGTAGAATA TTCAGCTGCA TCGGACGCCT ACACCCATTG CCCTGAAGGT 
TTTAGCGAGT TCTACAATCA ACGACGTAGA TGGGTGCCTT CGACCATTGC TAACATTATG 
GACTTGCTTG TGGATTATAA ACATACCATT AAAATCAATG ACAATATTTC CTCACCGTAC 
ATCGCATACC AGATGATGTT GATGGGCGGT ACGATTCTGG GACCTGGAAC TATATTTCTT 
ATGTTGGTGG GTGCTTTTGT GGCCGCTTTT AGAATTGATA ATTGGACATC CTTCGAATAC 
AATCTATATC CTATAATGTT GTTTATGTTT GTTTGTTTCA CAATGAAGTC AGAATATCAA 
TTATTGGTCG CGCAAATATT ATCAACGGCC TATGCTATGA TAATGATGGC TGTAATTGTG 
GGTACAGCAC TGCAATTAGG CGAGGACGGT ATCGGATCCC CATCCGCTAT ATTCTTGATT 
TCACTGTCAA GTTCTTTCTT TATAGCGGCC TGTTTACATC CCCAGGAGTT TTGGTGTATC 
GTTCCCGGTA TCATTTATCT ATTATCTATA CCCTCTATGT ACTTGCTTTT GATTTTGTAT 
TCTATTATAA ATCTGAATGT AGTATCGTGG GGTACGCGAG AAGTGCAAAC CAAGAAAACA 
AAGAAGGAAA TCGAGCAAGA AAAGAAAGAT GCCGAAGAGG CGAAAAAGAA GGCGAAACAA 
AAGTCCCTTC TTGGTTTCCT GCAGGGTGTC AATTCTAACG AAGAGGAAGG CTCCATAGAA 
CTATCGTTTG CTGGTCTTTT TAAATGTTTA CTGTGTACTC ATCCTAAAGG AAACGAAGAA 
AAAGTACAGC TCATGCATAT CGCTTCAACA CTTGAGAAAT TGGAAAAGAA AATTGAAAAC 
GTCGAGAAGG CCGTGGATCC GCACGGTCTA TCCAGAAGTC GCAAACTATC GCTAGGCCCT 
CGTGGTAGCA CTAACGGAGA CCACCAGTTG GACGCTCTTA ATGAGGACCC CGAAGAAGAA 
AACGATTCCG ATTCCGACAC CGGCACTCTA TCCACTGAAC CAAGGGAACG ACGAGATGAT 
CTTATAAATC CATATTGGAT TGAGGATCCC GGCTTGAAAA AAGGCGAAGT AGATTTCTTG 
AGCCCGCCCG AGGTACAATT CTGGAAAGAC CTTATTGACA AGTATTTATA TCCTATTGAC 
GAAGACAAGG ACGAGAAGGC ACGTATTTCG AGAGACTTAA AAGAACTGAG AGATTCTTCT 
GTATTTTCAT TCTTTATGAT CAATGCTCTT TTTGTGCTCA TTGTATTTTT GCTACAATTG 
AACAAGGACA ACCTTCACAT TAAATGGCCC TTCGGAGTTA AGACTAACAT TACCTATGAT 
GAGGTCTCAC AAGAGCTTCT CATCTCGAAG GAATACTTGC AATTAGAGCC TATCGGTCTG 
GTATTTGTGT TCTTCTTTGC CTTGATTTTG GTCATACAAT TTTCGGCCAT GTTGTTCCAT 
CGATTCGGAA CTATTTCGCA TATTCTATCA TCGACGGATC TCAACTGGTT CTGTTCAAAG 
AAATCGGAAG ATTTGTCACA GGATGCGCTT TTGGATAAGA ATGCAATAGC AATAGTGAAA 
GACCTTCAGA AATTAAACGG TCTGGACGAT GATTATGATA ACGATTCAGG ATCTGGACCA 
CACAACGTCG GCCGAAGGAA GACAATACAC AATTTAGAGA AAGCTAGACA GAAGAAACGT 
AACATAGGAA CACTAGATGT GGCTTTCAAG AAACGATTCT TCAATATGAA TGCTAATGAT 
GGTCCGGGTA CCCCAGTTTT AAATCGTAAA ATGACATTGA GAAGGGAGAC ATTGAAAGCT 
TTAGAGACCC GTCGTAACTC TGTAATGGCA GAAAGAAGGA AGTCGCAAAT GCAGACACTT 
GGCGCTAATA ATGAATACGG TGTTACTGGA ATGCTCAATA ATAACGTTGG ACCTCGTCAT 
AGAGCATCGA ATGCTAATAT TTCAGTGAAG GATGTATTCT CGGAGCCCAA CGGAGGACAG 
ATCAACAGGG CCTACGAAGC CTCACTCGGG GACGACGATG ACTCAAATTC CATGCGACTT 
CAGCCAAGAC AAAACCAAGT TTCATTCCAA GGGAGGTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA methylation mediates BmDeaf1-regulated tissue- and stage-specific expression of BmCHSA-2b in the silkworm, Bombyx mori.
Epigenetics & chromatin11(1)32(2018 06)
Xu G,Zhang J,Lyu H,Song Q,Feng Q,Xiang H,Zheng S

Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.
BMC genomics15820(2014 Sep)
Ou J,Deng HM,Zheng SC,Huang LH,Feng QL,Liu L

Chitin synthase A: a novel epidermal development regulation gene in the larvae of Bombyx mori.
Molecular biology reports41(7)4177-86(2014 Jul)
Zhuo W,Fang Y,Kong L,Li X,Sima Y,Xu S

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LOC100884166 cDNA ORF clone, Bombyx mori(domestic silkworm)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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