nAChRb2 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRb2
Entrez Gene ID	726079
Full Name	nicotinic acetylcholine receptor beta2 subunit
Synonyms	GB12006,nAChRbeta2
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001098229.1	NP_001091699.1	nicotinic acetylcholine receptor beta2 subunit

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera.
Jones AK, Raymond-Delpech V, Thany SH, Gauthier M, Sattelle DB
Genome research16(11)1422-30(2006 Nov)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nAChRb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00059	NM_001098229.1 Latest version!	Apis mellifera nicotinic acetylcholine receptor beta2 subunit (nAChRb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRb2 ( NM_001098229.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001091699.1 Apis mellifera nicotinic acetylcholine receptor beta2 subunit (nAChRb2), mRNA.

Price & Availability↑

CloneID	OHd00059
Clone ID Related Accession (Same CDS sequence)	NM_001098229.1
Accession Version	NM_001098229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1539705600000
Organism	Apis mellifera(honey bee)
Product	nicotinic acetylcholine receptor beta2 subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ026039.1. On May 25, 2007 this sequence version replaced XM_001121851.1. ##Evidence-Data-START## Transcript exon combination :: DQ026039.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01985569 [ECO:0000348] ##Evidence-Data-END##

ATGTTAAACA TGAAGAATAT ATTCCCCGTT TTATTCGTGA TCATTAATGT TTTATTACAT 
GGACAAGTGA TCTGTTTTGT TTGCAAAGAC ATCACAAGCA CTTCTGCACT TTACAGATTG 
AAACTGTATC TGTTCTGCGA TTACGACAGG GACATTATTC CCGAACAAAA AAATGCCACT 
AAAATCGATT TTGGATTAAG CATTCAACAT TATAACGTTG ATGAATATTC GCACACCGTC 
GATTTCCACG TTATGCTAAA ATTGATGTGG GAACAGAGTC ACCTCACGTG GAAATCATCC 
GAATTCGATT CTATCAATTC TATTCGCGTA AAGAGTTACG AAATCTGGGT CCCCGATATC 
GTGATGCACA GCGTAACAAG CGTCGGTATC GATCTTGAGA TGCCCTCTGT CGAATGCATA 
GTGTTCAATT CTGGCACTAT ACTTTGCGTA CCGTTCACTA CGTATACACC TGTTTGCGAA 
TATGATCACA CTTGGTGGCC TTACGATATA TTGAACTGCA CTATACACAT TGCCTCGTGG 
TCCCACGGAA GCAACGAGAT TAAATTAAAC TCTTTGGATA CTGAACAAAT TTTAGATGAT 
ATGTACAATA ATAACACGGA ATGGGAGATA GTGCATATGT CTCACAGCGA GAGCACGATA 
GATTCCAAAT TTGGTTTAGG TTTTACCACT GATTTGTTAT CTTATAATAT TCTGCTGAGA 
AGACATTACT CCATGAACAG TACGACGTAT GTGACTTTGA CCATAGTATT AATGACCATG 
ACATTGATGA CATTATGGTT AGAGCCAAGT TCTACGGAGC GTATGATCAT AGCAAATCTG 
AATTTTATTC TGCATCTATT TTGCTTGTTA GATGTGCAAT GGAGGATTCC TTTCAATGGA 
ATTCAAATGC CAAACTTGAT GGTGTTCTAT GAGAAATCTC TTGCCCTAGC CGCATTCTCG 
CTTATGTTGA CAAGCATCTT GCGATATTTG CAAGAATTAC ACGTTGACGC TCCAACATGG 
ATATCCTCCG TCACTGAATC GGTTTTAAAA AGTAAAATAG GACAAGTGTT TTTGATCACT 
ATTCTGGATT CAAAAGTATC GGCAAGGATC GAAATGAACG AGGATGATAA CACGAGTTTG 
GTATCTCTGG ATAAGAAACA GTATACATGG AGACACACGT CCGTTTTAAT CGGATGGAGT 
GCTTTTTTAT GCATTTCACT TGTTTATATA ATTATGCTAA TTATTTTTAT ACCAAGAAAC 
ATATATGAAA ATTTTATCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091699.1
CDS	1..1284
Translation	MLNMKNIFPVLFVIINVLLHGQVICFVCKDITSTSALYRLKLYLFCDYDRDIIPEQKNATKIDFGLSIQHYNVDEYSHTVDFHVMLKLMWEQSHLTWKSSEFDSINSIRVKSYEIWVPDIVMHSVTSVGIDLEMPSVECIVFNSGTILCVPFTTYTPVCEYDHTWWPYDILNCTIHIASWSHGSNEIKLNSLDTEQILDDMYNNNTEWEIVHMSHSESTIDSKFGLGFTTDLLSYNILLRRHYSMNSTTYVTLTIVLMTMTLMTLWLEPSSTERMIIANLNFILHLFCLLDVQWRIPFNGIQMPNLMVFYEKSLALAAFSLMLTSILRYLQELHVDAPTWISSVTESVLKSKIGQVFLITILDSKVSARIEMNEDDNTSLVSLDKKQYTWRHTSVLIGWSAFLCISLVYIIMLIIFIPRNIYENFIS

Target ORF information:

RefSeq Version	NM_001098229.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera nicotinic acetylcholine receptor beta2 subunit (nAChRb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098229.1

ORF Insert Sequence:

ATGTTAAACA TGAAGAATAT ATTCCCCGTT TTATTCGTGA TCATTAATGT TTTATTACAT 
GGACAAGTGA TCTGTTTTGT TTGCAAAGAC ATCACAAGCA CTTCTGCACT TTACAGATTG 
AAACTGTATC TGTTCTGCGA TTACGACAGG GACATTATTC CCGAACAAAA AAATGCCACT 
AAAATCGATT TTGGATTAAG CATTCAACAT TATAACGTTG ATGAATATTC GCACACCGTC 
GATTTCCACG TTATGCTAAA ATTGATGTGG GAACAGAGTC ACCTCACGTG GAAATCATCC 
GAATTCGATT CTATCAATTC TATTCGCGTA AAGAGTTACG AAATCTGGGT CCCCGATATC 
GTGATGCACA GCGTAACAAG CGTCGGTATC GATCTTGAGA TGCCCTCTGT CGAATGCATA 
GTGTTCAATT CTGGCACTAT ACTTTGCGTA CCGTTCACTA CGTATACACC TGTTTGCGAA 
TATGATCACA CTTGGTGGCC TTACGATATA TTGAACTGCA CTATACACAT TGCCTCGTGG 
TCCCACGGAA GCAACGAGAT TAAATTAAAC TCTTTGGATA CTGAACAAAT TTTAGATGAT 
ATGTACAATA ATAACACGGA ATGGGAGATA GTGCATATGT CTCACAGCGA GAGCACGATA 
GATTCCAAAT TTGGTTTAGG TTTTACCACT GATTTGTTAT CTTATAATAT TCTGCTGAGA 
AGACATTACT CCATGAACAG TACGACGTAT GTGACTTTGA CCATAGTATT AATGACCATG 
ACATTGATGA CATTATGGTT AGAGCCAAGT TCTACGGAGC GTATGATCAT AGCAAATCTG 
AATTTTATTC TGCATCTATT TTGCTTGTTA GATGTGCAAT GGAGGATTCC TTTCAATGGA 
ATTCAAATGC CAAACTTGAT GGTGTTCTAT GAGAAATCTC TTGCCCTAGC CGCATTCTCG 
CTTATGTTGA CAAGCATCTT GCGATATTTG CAAGAATTAC ACGTTGACGC TCCAACATGG 
ATATCCTCCG TCACTGAATC GGTTTTAAAA AGTAAAATAG GACAAGTGTT TTTGATCACT 
ATTCTGGATT CAAAAGTATC GGCAAGGATC GAAATGAACG AGGATGATAA CACGAGTTTG 
GTATCTCTGG ATAAGAAACA GTATACATGG AGACACACGT CCGTTTTAAT CGGATGGAGT 
GCTTTTTTAT GCATTTCACT TGTTTATATA ATTATGCTAA TTATTTTTAT ACCAAGAAAC 
ATATATGAAA ATTTTATCAG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

The nicotinic acetylcholine receptor gene family of the honey bee, Apis mellifera.
Genome research16(11)1422-30(2006 Nov)
Jones AK,Raymond-Delpech V,Thany SH,Gauthier M,Sattelle DB

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

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nAChRb2 cDNA ORF clone, Apis mellifera(honey bee)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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