DYSF gene cDNA ORF clone, Chlorocebus sabaeus(green monkey)

mRNA	Protein	Name
XM_007970262.1	XP_007968453.1	dysferlin isoform X2
XM_007970268.1	XP_007968459.1	dysferlin isoform X7
XM_007970269.1	XP_007968460.1	dysferlin isoform X8
XM_007970270.1	XP_007968461.1	dysferlin isoform X9
XM_007970261.1	XP_007968452.1	dysferlin isoform X1
XM_007970266.1	XP_007968457.1	dysferlin isoform X5
XM_007970272.1	XP_007968463.1	dysferlin isoform X11
XM_007970274.1	XP_007968465.1	dysferlin isoform X13
XM_007970276.1	XP_007968467.1	dysferlin isoform X15
XM_007970265.1	XP_007968456.1	dysferlin isoform X4
XM_007970275.1	XP_007968466.1	dysferlin isoform X14
XM_007970264.1	XP_007968455.1	dysferlin isoform X3
XM_007970273.1	XP_007968464.1	dysferlin isoform X12
XM_007970263.1	XP_007968454.1	dysferlin isoform X2
XM_007970267.1	XP_007968458.1	dysferlin isoform X6
XM_007970271.1	XP_007968462.1	dysferlin isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCh102343	XM_007970261.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102344	XM_007970262.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102344	XM_007970263.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102345	XM_007970264.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102346	XM_007970265.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102347	XM_007970266.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102348	XM_007970267.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102349	XM_007970268.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102350	XM_007970269.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102351	XM_007970270.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102352	XM_007970271.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102353	XM_007970272.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102354	XM_007970273.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102355	XM_007970274.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102356	XM_007970275.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCh102357	XM_007970276.1 Latest version!	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

DYSF ( XM_007970261.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968452.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCh102343
Clone ID Related Accession (Same CDS sequence)	XM_007970261.1
Accession Version	XM_007970261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACGATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA CGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGATGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGAAATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ACGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACCAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTCGGT GAGCCGGCCA CGGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TAGAGCTGTA CGACCATGAC ACTTACGGTG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAATATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT GCTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT ACAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGATACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAAGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCA GCGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACCTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCATG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC TAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT CTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968452.1
CDS	1213..7572
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970261.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970261.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACGATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA CGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGATGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGAAATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ACGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACCAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTCGGT GAGCCGGCCA CGGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TAGAGCTGTA CGACCATGAC ACTTACGGTG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAATATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT GCTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT ACAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGATACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAAGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCA GCGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACCTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCGGAGCATG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC TAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCCTGT CTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCCGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTCATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970262.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968453.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OCh102344
Clone ID Related Accession (Same CDS sequence)	XM_007970262.1 , XM_007970263.1
Accession Version	XM_007970262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACACGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGATGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG AAATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGACG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACCACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTCGGTGAG CCGGCCACGG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTAG AGCTGTACGA CCATGACACT TACGGTGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ATATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTGCT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATACA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGATACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAAGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCAGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACC 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCATGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACTAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTCTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968453.1
CDS	776..7132
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970262.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970262.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACACGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGATGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG AAATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGACG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACCACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTCGGTGAG CCGGCCACGG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTAG AGCTGTACGA CCATGACACT TACGGTGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ATATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTGCT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATACA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGATACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAAGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCAGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACC 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCATGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACTAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTCTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970263.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968454.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OCh102344
Clone ID Related Accession (Same CDS sequence)	XM_007970262.1 , XM_007970263.1
Accession Version	XM_007970263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACACGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGATGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG AAATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGACG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACCACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTCGGTGAG CCGGCCACGG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTAG AGCTGTACGA CCATGACACT TACGGTGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ATATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTGCT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATACA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGATACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAAGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCAGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACC 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCATGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACTAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTCTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968454.1
CDS	642..6998
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970263.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970263.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACACGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGATGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG AAATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGACG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACCACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTCGGTGAG CCGGCCACGG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTAG AGCTGTACGA CCATGACACT TACGGTGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ATATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTGCT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATACA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGATACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAAGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCAGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACC 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCATGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACTAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTCTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970264.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968455.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OCh102345
Clone ID Related Accession (Same CDS sequence)	XM_007970264.1
Accession Version	XM_007970264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACGATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCCTCAAGC CTTCGAAAGC CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CACGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGATG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGAAATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGACGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACC ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTCG GTGAGCCGGC CACGGTTGCT GAGCAGCCGC CCAGCATTGT GGTAGAGCTG 
TACGACCATG ACACTTACGG TGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAATATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTGCTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATACAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGATACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAAGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCAG CGCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CCTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGGT CACTCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCGC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CGTGGAGGAG 
CGTCTGGCTC TGCACGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCATGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACAAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACT AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTC TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968455.1
CDS	1213..7551
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEEETAGALKPSKASDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970264.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970264.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACGATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GACTGCAGGT GCCCTCAAGC CTTCGAAAGC CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CACGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGATG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGAAATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTACTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGATGTCACC 
GGCAAGATCA AGCTACCCAA GGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGACGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGATGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACC ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTTATCTT CTACGAGATC 
GAGATCTTCG GTGAGCCGGC CACGGTTGCT GAGCAGCCGC CCAGCATTGT GGTAGAGCTG 
TACGACCATG ACACTTACGG TGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAATATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG GTAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTGCTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATACAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGATACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAAGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCAG CGCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CCTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGGT CACTCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCGC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CGTGGAGGAG 
CGTCTGGCTC TGCACGTGCT TCAGCAGCAG GGCCTGGTCC CGGAGCATGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATTATCTGG AATACAAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACT AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCCTGTC TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CCGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTCATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970265.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968456.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OCh102346
Clone ID Related Accession (Same CDS sequence)	XM_007970265.1
Accession Version	XM_007970265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC CTCAAGCCTT CGAAAGCCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAC GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGATGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGAAATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA CGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACCACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TTATCTTCTA CGAGATCGAG 
ATCTTCGGTG AGCCGGCCAC GGTTGCTGAG CAGCCGCCCA GCATTGTGGT AGAGCTGTAC 
GACCATGACA CTTACGGTGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAATATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTG 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATA 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GATACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AAGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCAGCGC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACCT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGGTCAC TCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCGCAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCGT GGAGGAGCGT 
CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCATGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG CCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACAAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACCGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACTAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTCTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968456.1
CDS	776..7111
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEEETAGALKPSKASDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970265.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970265.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGAGGAGAC TGCAGGTGCC CTCAAGCCTT CGAAAGCCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAC GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGATGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGAAATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT ACTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA TGTCACCGGC 
AAGATCAAGC TACCCAAGGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA CGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGATGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACCACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TTATCTTCTA CGAGATCGAG 
ATCTTCGGTG AGCCGGCCAC GGTTGCTGAG CAGCCGCCCA GCATTGTGGT AGAGCTGTAC 
GACCATGACA CTTACGGTGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAATATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGGTA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTG 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATA 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GATACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AAGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCAGCGC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACCT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGGTCAC TCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCGCAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCGT GGAGGAGCGT 
CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC CTGGTCCCGG AGCATGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG CCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT TATCTGGAAT ACAAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACCGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACTAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCCTGTCTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCCG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTCATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970266.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968457.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OCh102347
Clone ID Related Accession (Same CDS sequence)	XM_007970266.1
Accession Version	XM_007970266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCCTC 
AAGCCTTCGA AAGCCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACACGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GATGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA AATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGACGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACCACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTCGGTGAGC CGGCCACGGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTAGA GCTGTACGAC CATGACACTT ACGGTGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA TATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTGCTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATACAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATAAGATCC TCCAGGAGGA AGAGTTTATC 
GATTGGTGGA GCAAATTCTT TGCCTCCATA GGGGAGAGGG AAAAGTGCGG CTCCTACTTG 
GAGAAGGATT TTGATACCCT GAAGGTCTAT GACACACAGC TGGAGAATGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTGTACC GGGGCAAGAC GCAAGAGGAG 
ACAGAAGATC CATCTGTCAT TGGCGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA 
GAAGACCCAG CCATCCCCAT GCCCCCAAGA CAGTTCCACC AGCTGGCAGC GCAGGGACCC 
CAGGAGTGCT TGGTCCGTAT CTACATTGTC CGAGCATTTG GCCTGCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TCCCCTGCAC CTTGGAGCCT GTGTTTGGAA AGATGTTTGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC ACTCTCTACG ACTATGACCT CCTCTCCAAG 
GATGAAAAGA TTGGTGAGAC GGTCATCGAC CTGGAGAACA GGCTGCTGTC CAAGTTTGCG 
GCTCGCTGTG GACTCCCGCA GACCTACTGT GTCTCTGGAC CGAACCAGTG GCGGGACCAG 
CTCCGCCCCT CCCAGCTCCT CCACCTCTTC TGCCAGCAGC GCAGAGTCAA GGCGCCTGTG 
TACCGGACAG ACCGTGTGAT GTTTCAGGAT AAAGAATACT CCATTGAAGA AATAGAGGCT 
GGCAGGATCC CAAACCCACA CCTGGGCCCC GTGGAGGAGC GTCTGGCTCT GCACGTGCTT 
CAGCAGCAGG GCCTGGTCCC GGAGCATGTG GAGTCACGGC CCCTCTACAG CCCCCTGCAG 
CCAGACATTG AGCAGGGGAA GCTGCAGATG TGGGTCGACC TGTTTCCGAA GGCCCTGGGG 
CGCCCTGGAC CTCCCTTCAA CATCACCCCA CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATTATCTGGA ATACAAGAGA TGTGATCCTG GATGACCTGA GCCTCACGGG GGAGAAGATG 
AGCGACATTT ATGTGAAAGG TTGGATGATT GGCTTTGAAG AACACAAGCA AAAGACAGAC 
GTGCATTATC GCTCCCTGGG AGGTGAAGGC AACTTCAACT GGAGGTTCAT TTTCCCCTTC 
GACTACCTGC CGGCTGAGCA AGTCTGTACC GTTGCCAAGA AGGATGCCTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACTA GTGATATTCC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ATGATTTTCT GGGCTCCCTG CAGCTCGATC TCAACCGCAT GCCCAAGCCA 
GCCAAGACAG CCGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCAGAATGG 
TTTGTGTCCC TTTTTGAGCA GAAAACAGTG AAGGGCTGGT GGCCCTGTCT AGCAGAAGAG 
GGTGAGAAGA AGATACTGGC GGGCAAGCTG GAAATGACCT TGGAGATTGT AACAGAGAGT 
GAGCATGAGG AGCGGCCCGC TGGCCAGGGC CGGGATGAGC CCAACATGAA CCCTAAGCTT 
GAGGACCCAA GGCGCCCCGA CACCTCCTTC CTGTGGTTCA CCTCCCCATA CAAGACCATG 
AAGTTCATCC TGTGGCGGCG TTTCCGGTGG GCCATCATCC TCTTCATCAT CCTCTTCATC 
CTGCTGCTGT TCCTGGCCAT CTTCATCTAC GCCTTCCCGA ACTATGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968457.1
CDS	776..7093
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEEETAGALKPSKASDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHKILQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970266.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970266.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCCTC 
AAGCCTTCGA AAGCCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACACGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GATGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA AATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGACGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACCACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTCGGTGAGC CGGCCACGGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTAGA GCTGTACGAC CATGACACTT ACGGTGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA TATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTGCTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATACAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATAAGATCC TCCAGGAGGA AGAGTTTATC 
GATTGGTGGA GCAAATTCTT TGCCTCCATA GGGGAGAGGG AAAAGTGCGG CTCCTACTTG 
GAGAAGGATT TTGATACCCT GAAGGTCTAT GACACACAGC TGGAGAATGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTGTACC GGGGCAAGAC GCAAGAGGAG 
ACAGAAGATC CATCTGTCAT TGGCGAATTT AAGGGCCTCT TCAAAATTTA TCCCCTCCCA 
GAAGACCCAG CCATCCCCAT GCCCCCAAGA CAGTTCCACC AGCTGGCAGC GCAGGGACCC 
CAGGAGTGCT TGGTCCGTAT CTACATTGTC CGAGCATTTG GCCTGCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TCCCCTGCAC CTTGGAGCCT GTGTTTGGAA AGATGTTTGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC ACTCTCTACG ACTATGACCT CCTCTCCAAG 
GATGAAAAGA TTGGTGAGAC GGTCATCGAC CTGGAGAACA GGCTGCTGTC CAAGTTTGCG 
GCTCGCTGTG GACTCCCGCA GACCTACTGT GTCTCTGGAC CGAACCAGTG GCGGGACCAG 
CTCCGCCCCT CCCAGCTCCT CCACCTCTTC TGCCAGCAGC GCAGAGTCAA GGCGCCTGTG 
TACCGGACAG ACCGTGTGAT GTTTCAGGAT AAAGAATACT CCATTGAAGA AATAGAGGCT 
GGCAGGATCC CAAACCCACA CCTGGGCCCC GTGGAGGAGC GTCTGGCTCT GCACGTGCTT 
CAGCAGCAGG GCCTGGTCCC GGAGCATGTG GAGTCACGGC CCCTCTACAG CCCCCTGCAG 
CCAGACATTG AGCAGGGGAA GCTGCAGATG TGGGTCGACC TGTTTCCGAA GGCCCTGGGG 
CGCCCTGGAC CTCCCTTCAA CATCACCCCA CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATTATCTGGA ATACAAGAGA TGTGATCCTG GATGACCTGA GCCTCACGGG GGAGAAGATG 
AGCGACATTT ATGTGAAAGG TTGGATGATT GGCTTTGAAG AACACAAGCA AAAGACAGAC 
GTGCATTATC GCTCCCTGGG AGGTGAAGGC AACTTCAACT GGAGGTTCAT TTTCCCCTTC 
GACTACCTGC CGGCTGAGCA AGTCTGTACC GTTGCCAAGA AGGATGCCTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACTA GTGATATTCC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ATGATTTTCT GGGCTCCCTG CAGCTCGATC TCAACCGCAT GCCCAAGCCA 
GCCAAGACAG CCGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCAGAATGG 
TTTGTGTCCC TTTTTGAGCA GAAAACAGTG AAGGGCTGGT GGCCCTGTCT AGCAGAAGAG 
GGTGAGAAGA AGATACTGGC GGGCAAGCTG GAAATGACCT TGGAGATTGT AACAGAGAGT 
GAGCATGAGG AGCGGCCCGC TGGCCAGGGC CGGGATGAGC CCAACATGAA CCCTAAGCTT 
GAGGACCCAA GGCGCCCCGA CACCTCCTTC CTGTGGTTCA CCTCCCCATA CAAGACCATG 
AAGTTCATCC TGTGGCGGCG TTTCCGGTGG GCCATCATCC TCTTCATCAT CCTCTTCATC 
CTGCTGCTGT TCCTGGCCAT CTTCATCTAC GCCTTCCCGA ACTATGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970267.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968458.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OCh102348
Clone ID Related Accession (Same CDS sequence)	XM_007970267.1
Accession Version	XM_007970267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
CTCAAGCCTT CGAAAGCCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAC GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGATGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGAAATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA CGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACCACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTCGGTG AGCCGGCCAC GGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT AGAGCTGTAC GACCATGACA CTTACGGTGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAATATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTG CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATA CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGATACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAAGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCAG CGCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CCTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACTCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCGC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CGTGGAGGAG CGTCTGGCTC TGCACGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCATGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACAAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACT AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTC TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968458.1
CDS	1213..7521
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEEETAGALKPSKASDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970267.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970267.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
CTCAAGCCTT CGAAAGCCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAC GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGATGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGAAATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA CGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACCACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTCGGTG AGCCGGCCAC GGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT AGAGCTGTAC GACCATGACA CTTACGGTGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAATATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTG CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATA CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGATACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAAGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCAG CGCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CCTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACTCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCGC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CGTGGAGGAG CGTCTGGCTC TGCACGTGCT TCAGCAGCAG 
GGCCTGGTCC CGGAGCATGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATTATCTGG 
AATACAAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACT AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCCTGTC TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CCGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTCATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970268.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968459.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OCh102349
Clone ID Related Accession (Same CDS sequence)	XM_007970268.1
Accession Version	XM_007970268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCCTC 
AAGCCTTCGA AAGCCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACACGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GATGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA AATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGACGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACCACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTCGGTGAGC CGGCCACGGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTAGA GCTGTACGAC CATGACACTT ACGGTGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA TATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTGCTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATACAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GATACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AAGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCAGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACCT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCATGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACTAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTCTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968459.1
CDS	776..7081
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEEETAGALKPSKASDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970268.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970268.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCCTC 
AAGCCTTCGA AAGCCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACACGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GATGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA AATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGACGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACCACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTCGGTGAGC CGGCCACGGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTAGA GCTGTACGAC CATGACACTT ACGGTGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA TATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTGCTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATACAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GATACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AAGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCAGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACCT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCATGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACTAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTCTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970269.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968460.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OCh102350
Clone ID Related Accession (Same CDS sequence)	XM_007970269.1
Accession Version	XM_007970269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACGATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA CGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGATGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGAAATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ACGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACCAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTCGGT GAGCCGGCCA CGGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TAGAGCTGTA CGACCATGAC ACTTACGGTG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAATATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT GCTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT ACAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGATACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAAGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCAGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACC 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCATGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACTAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTCTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968460.1
CDS	1213..7509
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970269.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970269.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGCCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACGATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCCGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA CGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGATGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGAAATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TACTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ATGTCACCGG CAAGATCAAG CTACCCAAGG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ACGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGATGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACCAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTTATCTTCT ACGAGATCGA GATCTTCGGT GAGCCGGCCA CGGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TAGAGCTGTA CGACCATGAC ACTTACGGTG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAATATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGGT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT GCTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT ACAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGATACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAAGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCAGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACC 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCG 
GAGCATGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTGTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACTAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCCTGTCTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCC GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTCATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970270.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968461.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OCh102351
Clone ID Related Accession (Same CDS sequence)	XM_007970270.1
Accession Version	XM_007970270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACACGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGATGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG AAATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGACG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACCACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTCGGTGAG CCGGCCACGG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTAG AGCTGTACGA CCATGACACT TACGGTGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ATATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTGCT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATACA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA TACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAA GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCAGCGCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACCTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGGTCACTC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCGCAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CATGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGCC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC AAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACCGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACTAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTCTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968461.1
CDS	775..7068
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970270.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970270.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGC CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACGATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCCGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGAAATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACACGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGATGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG AAATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTAC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGATG TCACCGGCAA GATCAAGCTA CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGACG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ATGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACCACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTT 
ATCTTCTACG AGATCGAGAT CTTCGGTGAG CCGGCCACGG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTAG AGCTGTACGA CCATGACACT TACGGTGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ATATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTGCT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATACA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA TACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAA GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCAGCGCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACCTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGGTCACTC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCGCAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG 
CATGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGCC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTGTATTA TCTGGAATAC AAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACCGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACTAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC CTGTCTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCCGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT CATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970271.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968462.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OCh102352
Clone ID Related Accession (Same CDS sequence)	XM_007970271.1
Accession Version	XM_007970271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACACGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGATGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG AAATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGACG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACCACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTCGGTGAG 
CCGGCCACGG TTGCTGAGCA GCCGCCCAGC ATTGTGGTAG AGCTGTACGA CCATGACACT 
TACGGTGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ATATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTGCT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATACA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGATACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAAGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCAGCG 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACC TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CTCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG 
CACGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCATGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GCCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACAAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACCG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACTAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTCTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968462.1
CDS	1213..7479
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970271.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970271.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACACGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGATGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG AAATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGACG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACCACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTCGGTGAG 
CCGGCCACGG TTGCTGAGCA GCCGCCCAGC ATTGTGGTAG AGCTGTACGA CCATGACACT 
TACGGTGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ATATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTGCT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATACA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGATACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAAGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCAGCG 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACC TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CTCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG 
CACGTGCTTC AGCAGCAGGG CCTGGTCCCG GAGCATGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GCCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTGTA TTATCTGGAA TACAAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACCG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACTAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCCTGTCTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCC GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTCATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970272.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968463.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OCh102353
Clone ID Related Accession (Same CDS sequence)	XM_007970272.1
Accession Version	XM_007970272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACACGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGA TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGAAA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGACGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CCACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT CGGTGAGCCG 
GCCACGGTTG CTGAGCAGCC GCCCAGCATT GTGGTAGAGC TGTACGACCA TGACACTTAC 
GGTGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAATA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTGCTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATACAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA TACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAA 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCAGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACCTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CATGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACTAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTCTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968463.1
CDS	775..7038
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970272.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970272.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACACGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGA TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGAAA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGACGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CCACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT CGGTGAGCCG 
GCCACGGTTG CTGAGCAGCC GCCCAGCATT GTGGTAGAGC TGTACGACCA TGACACTTAC 
GGTGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAATA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTGCTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATACAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA TACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAA 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCAGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACCTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CATGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACTAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTCTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970273.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968464.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OCh102354
Clone ID Related Accession (Same CDS sequence)	XM_007970273.1
Accession Version	XM_007970273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
CTCAAGCCTT CGAAAGCCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAC GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGATGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGAAATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA CGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACCACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTCGGTG AGCCGGCCAC GGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT AGAGCTGTAC GACCATGACA CTTACGGTGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAATATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTG CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATA CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GATACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AAGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCAGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACCT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCATGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACTAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTCTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968464.1
CDS	1213..7458
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEEETAGALKPSKASDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970273.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970273.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGAGGAGAC TGCAGGTGCC 
CTCAAGCCTT CGAAAGCCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAC GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGATGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGAAATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT ACTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCACCGGC AAGATCAAGC TACCCAAGGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA CGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGATGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACCACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA CGAGATCGAG ATCTTCGGTG AGCCGGCCAC GGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT AGAGCTGTAC GACCATGACA CTTACGGTGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAATATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGGTA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTG CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATA CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GATACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AAGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCAGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACCT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCGG AGCATGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACTAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCCTGTCTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCCG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTCATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970274.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968465.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OCh102355
Clone ID Related Accession (Same CDS sequence)	XM_007970274.1
Accession Version	XM_007970274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCCTC 
AAGCCTTCGA AAGCCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACACGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GATGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA AATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGACGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACCACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTCGGTGAGC CGGCCACGGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTAGA GCTGTACGAC CATGACACTT ACGGTGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA TATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTGCTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATACAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAT 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAAG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCAGCGCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACCTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACTCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCGCAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCGTGGA GGAGCGTCTG GCTCTGCACG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ATGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGCCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACA 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACCGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACTAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTCTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968465.1
CDS	775..7017
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEEETAGALKPSKASDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970274.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970274.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG AGGAGACTGC AGGTGCCCTC 
AAGCCTTCGA AAGCCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACACGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GATGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA AATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTACT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGATGT CACCGGCAAG ATCAAGCTAC CCAAGGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGACGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA TGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACCACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTACGA GATCGAGATC TTCGGTGAGC CGGCCACGGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTAGA GCTGTACGAC CATGACACTT ACGGTGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA TATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGGTAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTGCTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATACAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAT 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAAG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCAGCGCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACCTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACTCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCGCAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCGTGGA GGAGCGTCTG GCTCTGCACG TGCTTCAGCA GCAGGGCCTG 
GTCCCGGAGC ATGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGCCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATTAT CTGGAATACA 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACCGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACTAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCC TGTCTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCCGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTC ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970275.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968466.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OCh102356
Clone ID Related Accession (Same CDS sequence)	XM_007970275.1
Accession Version	XM_007970275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACACGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGATGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG AAATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGACG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACCACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTCGGTGAG 
CCGGCCACGG TTGCTGAGCA GCCGCCCAGC ATTGTGGTAG AGCTGTACGA CCATGACACT 
TACGGTGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ATATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTGCT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATACA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA TACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAA 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCAGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACCTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CATGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACTAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTCTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968466.1
CDS	1213..7416
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970275.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970275.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AATCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCGA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCCCC TACTCCTCTG 
GAGCCCTCCC CGACGCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGC 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACGATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCCGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGAAATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACACGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGATGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG AAATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTAC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGATG TCACCGGCAA GATCAAGCTA 
CCCAAGGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGACG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ATGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACCACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTT ATCTTCTACG AGATCGAGAT CTTCGGTGAG 
CCGGCCACGG TTGCTGAGCA GCCGCCCAGC ATTGTGGTAG AGCTGTACGA CCATGACACT 
TACGGTGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ATATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGGTAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTGCT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATACA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA TACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAA 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCAGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACCTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCGGAG CATGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTGTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACTAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC CTGTCTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCCGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT CATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_007970276.1 ) cDNA ORF clone, Chlorocebus sabaeus(green monkey) -> XP_007968467.1 Chlorocebus sabaeus dysferlin (DYSF), transcript variant X16, mRNA.

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CloneID	OCh102357
Clone ID Related Accession (Same CDS sequence)	XM_007970276.1
Accession Version	XM_007970276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1399910400000
Organism	Chlorocebus sabaeus(green monkey)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006735260.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chlorocebus sabaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACACGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGA TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGAAA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGACGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CCACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT CGGTGAGCCG 
GCCACGGTTG CTGAGCAGCC GCCCAGCATT GTGGTAGAGC TGTACGACCA TGACACTTAC 
GGTGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAATA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTGCTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATACAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGATAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAAGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC AGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACCTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAT GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CTAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TCTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007968467.1
CDS	775..6975
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGEIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEEISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANYCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDHKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPALVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCLAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_007970276.1
Organism	Chlorocebus sabaeus(green monkey)
Definition	Chlorocebus sabaeus dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007970276.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCGACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCCCCTAC TCCTCTGGAG 
CCCTCCCCGA CGCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGCCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC GATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC CGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GAAATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACACGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGA TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGAAA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTACTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGATGTCA CCGGCAAGAT CAAGCTACCC 
AAGGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGACGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGATG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CCACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTTATC TTCTACGAGA TCGAGATCTT CGGTGAGCCG 
GCCACGGTTG CTGAGCAGCC GCCCAGCATT GTGGTAGAGC TGTACGACCA TGACACTTAC 
GGTGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAATA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGGTAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTGCTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATACAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGATAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAAGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC AGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACCTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCGGAGCAT GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TGTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CTAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCCTG TCTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCCGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTCAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	103220071
Full Name	dysferlin
Gene Type	protein-coding
Organism	Chlorocebus sabaeus(green monkey)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Chlorocebus sabaeus(green monkey)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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