TGME49_273478 cDNA ORF clone, Toxoplasma gondii ME49

Gene
Clones
gRNAs

Summary

Gene Symbol	TGME49_273478
Entrez Gene ID	29769478
Full Name	dynein heavy chain, n-terminal region 2 protein
Synonyms	TGME49_073480
Gene Type	protein-coding
Organism	Toxoplasma gondii ME49

mRNA and Protein(s)

mRNA	Protein	Name
XM_018781690.1	XP_018636726.1	dynein heavy chain, n-terminal region 2 protein

Pathways from BioSystems
Gene Ontology

The following TGME49_273478 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGME49_273478 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi10130	XM_018781690.1 Latest version!	Toxoplasma gondii ME49 dynein heavy chain, n-terminal region 2 protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGME49_273478 ( XM_018781690.1 ) cDNA ORF clone, Toxoplasma gondii ME49 -> XP_018636726.1 Toxoplasma gondii ME49 dynein heavy chain, n-terminal region 2 protein partial mRNA.

Price & Availability↑

CloneID	OTi10130
Clone ID Related Accession (Same CDS sequence)	XM_018781690.1
Accession Version	XM_018781690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1476806400000
Organism	Toxoplasma gondii ME49
Product	dynein heavy chain, n-terminal region 2 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_031476). COMPLETENESS: incomplete on both ends.

ATGGAAGAGC GCAGGGCATC CTCGACAAAT CACAGCGAGT GCCCTTCGGC TGCAGCAATT 
GAGTACAACA AACCACTTTT CTTGTTTTTA GATGGGCCGC TTAAGCTGAG TCTCGAAGAT 
TTCGACGATC CAGACCTGTA CGCAGATCCC TCTCCGCAGG AATGGGTCCG TCGCTGTGCT 
AGCCGGCGAA ACAAGGAGGC TCTGTGTCGG CTGGGACATT CTGCCAGTAA CGAGGCCTCA 
GCTCACTCTT GCATCTCTGC TACTCACTCA CGCCGCGGTT CTGTCAATGG CAAGCTAGAC 
GATAACAACG ACGGGAGGAA CACTAGTGGT GATGACCGTG CTCCGCGCTC GGGCTCCGAG 
GGCGGTTCTG ACACTCTCTC TGTCAGAAAT GGGGCTCCGC ACGAAACGGA CGAAAATTTG 
CCGAAAAGGG TGGTAGGACT GTCTGACGGT GTCTCAATCA TGGAAAGACA CACGTCGGAT 
GCAGTCTGCG AACACCTACA GTTTGACGAA CGGTGTTCGA AGGATGCAGA CGTGCTACAT 
TTCCTCGATG GCGACTGGCG GATGACCCCT TGTTGGGTTA CAGACTATTT CAAAGACGAA 
AAGAAATTCA AAGTGCGGTT GAAAGTGGAC GGGACCGAGA AAACCGTGCA TCGCCTCGCG 
ATCCGATTCG TTGGTGAAGA TCCAGTAAAG TGTCAAGAAA GAGCAGAACG ATGCCGACGC 
AGGCGGGACC AGATGCGTCT GCGCCAGTCT CTTATCAACC TCATTCACGG TCTCCAGGAC 
TGTGAGTTCC CACCGCTAGG AACACACGCC AGACAGCGAT TGCTGAAGCG CATTTTGCTC 
AAGGGTCGAG GCTGTCTGCG AAAGCTCGAT CATGGATATG TCCGTGACTT GTTCCGTGAG 
ATGGAAGACC TTCATATGTT CGCCATGAAG TTCGCAGCAG TCCGACACAA AACGCTTACC 
CTGAATGGTA CTCCCTACGC GTTTCTCACT CCTGAGAAGC TGCAGTCGAG ATTTGGATCT 
CTCTTCGTTC CTCTCCTTCC TTCGGCGATG AAGGAAAAGG GATGCGAACC TGTCTCCGGC 
ATGCAGACAC TCTCGAGAAC CCGGGCGCTG CTCAGGCGGA GTCCACATTT CAAGAAGGGA 
ATCACTAAGG CTTTCAAACT GGCTCAGTTC ATTCAACACC AGCAAGACCA CTGCGCAGAT 
GTTCAGGATG TCCTTGAAGA ACATTGGCGT GAATATATTT ACAATGAGAC CATGGCGACT 
CTTGGCGCAT TAGAAGGCGA ATACCATTTC TACGTCGACG ACATGGAGAA ATACTTGCGG 
AGCGACCTGC GCAAGATTCT GCGTAAAATT GACATCATCC TCTCACATCA CATGATGGAA 
TTCGTTCGGA CCTCGATTGC CGACTGGAAA CACTTCTTCC TCTCTTTCGT CGCCGACGAA 
ACACAACGCC CCGCTGACCC TCTCCTCATT CTTGATGTCC GCGCCACAGA AAATGAGAAG 
GAGCTGCTTC CCTTTCTTCA GTTGCCAAAT CCACCATTGT ACGAGATTTC GGCGACGTAT 
GAACCGCTGA ATGAGACTCG AGAGGCAGTC CAAAGCGTGC TGCGAACGTG TCTAGGAAAG 
GCAGAAAACT ACATCGCTCT CTTCAACAAG TTTGCATATT TATCCAGTGA TGTTGCAGAG 
GGCGCAAAGA ACGCTCACGT CGACTTTGAT CCGTCAGACC TTCCGAGTGT CCGCGAACGT 
CTAGAGAAAT ACCACACTGC AATGTTTGAA ATTGAGACGG TCTGCCCGAC AGAAGTCAAA 
CTGCAGATGA TCGTACTGGA TTGCTCGCAC GTGAAAGAAA CACTTTCGGC CAAGGCGGAA 
AAGCTCTGGA AAGCTGAATG CGAGAGAGTG TCTAAGTTCA TAGAGGATGA AATAGCTGCT 
GTCGTGAGAG AGTGGAACGA CCTCCATGAC CGCGTCTTGA CTATTCCAAC GACAGAGGAA 
GAGCTAGGTT CCTTAAAAAA CCTCATCGCC ACAATAAATG AATTGTCGGA TCCCCTGACG 
TCAAGAACGA GGTTCATTCA TGAAGCCATC GACCTACTCG CGTCGGTCAA TTTCGCCATC 
AACAAAACTG TGCACGAAGA GGCCTACAGG GCCTTCAGCT GGCCTCTCCA GATTAAATTC 
GACCTTGTGG AAACGGGTGG AATTCTTGAA AAAGGACGAC TGCGGTTTGA GGAGAAATTG 
GAAGCGGACA AGGCAGAGTT GGAGAAGGAC TTGAGCCAGC TGGAGCAAGA AGTTGATTTC 
GTCCGAAACG AGATGGGCGA CACCGAGCAG GCGATGGAAT ATTACAAACG AATCGTGCAG 
CTGCAGGAAC AACTGACGGC CGCCAAGAAC CGAACTGAGG CCTTTAAGAA GGCAGAAACT 
CTGTTTGGCT TGGAGGAACC GTCCGACTAC AGTCGTGTCG ACGACCTTTA TGCAGCCTTC 
GAGCCTTTCA GAAAATTGTG GGCGTTGGTC GTCGACTTCA AGTACGGTGA ACTTCAGTGG 
CTGAGCGCCG CCATCACGTC TTTGGATCCG GCGGAGGTCG AGACGAGTTT GGATCGATGG 
AGTCAAGAGG CGTACAAGTT GCGGAAGCAC TTTCTCACAG ATTCACGTTC CGTGCAGGTC 
GGAGTCGCGG ATGCGTTGCA CCTCTCCATA GAACGCTTTC GTCAGCATCT TCCTGTGATT 
CGTCCTCTCT GCAACGAGGC GTTTTTACCG CGGCACTGGG TCGATCTGCT GAAAACCATT 
GGTTGCGACC TAGACGTCGA CGAGGGCTTC TCCCTCACTC AACTTCTCGA CAGCGGGATC 
CAAGACCATC TACAGATCGT CGAAGAAAAA AGCGAGCTCG CCCAGAAGGA GTTCACGTTG 
AAGAACGCCC TGGTGAAGAT GAAGGTCGAG TGGAAAGCGA TGCAGCTCCA ACTCATCCCT 
TTCAAAGACA CACACACGTA CGTCATGAAA GGCTTTGATG TCGTCCAGGC TCTTCTGGAC 
GACCACCTCG TACGCACGCA GACGATGCGC GGCTCGCGCT TCATCCGGTG CATAGAATAC 
CAAAGTCGAG AGTGGGAATC GAAGTTGCTG GAAACCCAAC AGGCTCTCGA CAGTCTCCAA 
GCCTGCCAAC GTTCCTGGAT GTACCTCCAG CCGATTTTCC AGAGCGCAGA CATCTCGCAG 
CAAATTCCAC ACGAGGCAGG GCTGTTCCGA GAGGTCGATG ACCTGTGGAG ACGCACCGTC 
GCCCAAGCCG AAGAGACACC CGGAGTTCTG GATATCGTGG AATTCGGCAA TCTCCACCAC 
GACTTCACGC AAGCAAATAC GAAGCTAGCG CAGATTATGA GGGGCGTGAA CGACTTCCTC 
GAGACCAAGC GCCTCGCCTT CCCCAGATTC TTCTTCCTCT CCAACGAGGA GCTCCTCGCG 
ATCCTGTCGC AAACGACGGA CCTGTCACCC GTTCAAATGT ATTTGAACAA ATGCTTCGAG 
GGGATTCACA GAGTCGCTTT CGACGAACAT GGGACGGTCA TAAACGCCAT ACGAAGCGCT 
GCCGGAGAAG AAATTCCTCT CGGACGCCAC GTGGCTCTCG TGGACGGATC CCGATTTGTC 
GGAGCTGAAC ACTGGCTTGG CGAGGTTGAA AGCTCTGTCA GTGACAGCCT GCGGGATGTC 
ATGGAAGCTG CGATGAAAGA TTACCAGATA CGACCCAGAA CAGAATGGTG TTTATCCTGG 
CCCGGTCAGG TCGCCCTAAC GGCCAGCCAG ATCTTCTGGA CTTCAGAGGT CGCGGACGCG 
ATCAATGGTG CCCAACTAGC TAGCTACCTG GACAAATGCA ATGATCAACT TCAGGGACTC 
GTTCGTCTGG TCCGCGGCCG CTTAACGCAG CTGAATCGCC TGACAGTGAC GACTCTTCTG 
ACCATCGACG TGCATGCGCG GGACGTGGTG GAAGAGTTGA AGAAGGAGAA CACGTCTTCT 
TTGTCGGATT TCGAGTGGCT CGCGCAATTG CGTCTGTCGT GGCAGCAGCC AGGAAGTGTC 
ACTTTGCTGA CGGGAAAGAG AAACACAAAG CCGGAGATTC AGCTGAGCAT GATCAACGCG 
CGAATCTTTT ACGGATTTGA ATACTTGGGC AGCACGGAAA GACTCGTCAT CACGCCCCTA 
ACTGACCGGT GCTACCGAAC TCTGATCGGC GCTTTCCACT TGCAGTACGG CGGCGCACCG 
GAAGGTCCAG CGGGGACAGG AAAAACTGAA ACGACCAAAG ACTTGGCAAA AGCTGCGGGT 
ATGCAGTGTC TCGTTTTCAA CTGCTCTGAC GGTCTCGACT ACGTGTCGAT TGGGCGCTTC 
TTCAAGGGCC TGGTTTCCTC GGGAGCTTGG TGCTGCTTCG ACGAATTCAA CCGAATCGAA 
GTCGAAGTTC TGTCGGTCAT TGCTCAACAA GTGCATGCAA TTCAACAGGC CATACGCAAC 
CAGGTTAAAG TTTTTCTTTT TGAAGGGACG GATGTGAAAC TCGTACCCTC GTGCGCCATC 
AATATTACCA TGAATCCGGG CTATGCCGGA AGATCCGATC TACCAGACAA CCTGAAGGCT 
CTCTTCCGAC CTTGCGCGAT GATGATTCCG GACTTTTCGT TGATTGCGGA AGTGATGCTA 
TACTCATATG GATTTGCAGA CGCCCGGAAT GTGGCTCGCA AGGCCCTGGC GTGCCTCCGT 
CTGTCGTCAG AGCAGCTGTC ATCGCAAGAC CACTACGACT TTGGTATGCG CGCTCTGAAG 
GCGATTCTTA CCGCAGCCGG GCAGTTGAAA AGAACGTTTG GATCAGCGTT TGAGGAAGAC 
GAACTGACCC TGTACGCGCT GGAGAGGGTC AGCGTTCCCA AGCTTACTCC CAACGATACT 
CAGCTCTTCC GTGACATCGT TTCAGATCTG TTTCCAGGGG TGAAAGTGGC CTCTGAAGAC 
AACGGCTTTC TGCTTTCTCG TATCGAGCAG GTTGCGAAGA AAAAGAACCT TCAACCTACA 
GAGGCGTTTC TAACAAAGTG CATTCAACTC TGGGACACCA TCAAAGTGAG GCACGGGCTC 
ATGATTGTGG GGGCGACACT GACGGGGAAA ACGGCGGTGC TGGAGTGTCT CGCGGAGGCT 
CTTGGACGCG CCTGCGAAGG CCGAGAACGC GAACGGGAAA ACGGAGGCAG TGATCGCGAA 
GATCTATTCA GAGACACCAA AGAGAGACAC GAAGGGAGGA AAAGGAGAGA CAGGCAACGC 
AACGCTGCCA GGCAGCCGTT GGAGGCCGAC GAAGGCCGAG TTGCAGTAGC GCAAGAGATA 
AGCGACGAAG ACGAATGGGA GGAAGAACCC AACGGAGACG AAACAGAAGA AGAAGGTCGA 
GAGGGAGCCC AGCAGGAAAA AGACGAAGGA GGTCGCCGAC GTGGAAAGGA AGAGGGGGAA 
AATGGGACAG TACCTCACGA ATACTTGCCA TGCAAACTGT ATAAAATCAA TCCGAAAAGT 
GTTACACAGG GACAGCTTTA CGGCAAATAC GATGAAAACA CGCAAGAGTG GACAGACGGA 
GTTCTTGCAA TCGCTATTCG GGCTGCCTCC AACGAAAGCG ATGTTCAAGC CCGGCAGTGG 
ATCGTCCTTG ACGGCCCCGT CGATGCGGTG TGGATTGAGA ACATGAATAC GGTCTTGGAC 
GACAACAAAA GGCTTTGTCT CACGAGCGGA GAGATCATCA AACTCTCGGC CCTGACTACG 
ATGATCTTCG AAGTCGATGA CCTCTCCTCT GCTTCTCCAG CCACTGTCTC CCGTTGCGGG 
ATGGTCTACC TTGATCAAGC GCAGTGCACT TGGGAGCCGA TGGTGATTTC CTGGATCCAT 
CAGCAGAGGC AGAAAGCACC AAACTCCCTT CCGCTGTCCT GCATAGACGA AAGCGAGTCC 
GGGTTGTCTC TCACAGACTT GTTTCTCCAT GCTCTCCCGA TCTTCCTTCA ATTTATAAAG 
GTCAACAGTA CAACGCCTTG CGTCGTGTCA GATAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018636726.1
CDS	1..5982
Translation	MEERRASSTNHSECPSAAAIEYNKPLFLFLDGPLKLSLEDFDDPDLYADPSPQEWVRRCASRRNKEALCRLGHSASNEASAHSCISATHSRRGSVNGKLDDNNDGRNTSGDDRAPRSGSEGGSDTLSVRNGAPHETDENLPKRVVGLSDGVSIMERHTSDAVCEHLQFDERCSKDADVLHFLDGDWRMTPCWVTDYFKDEKKFKVRLKVDGTEKTVHRLAIRFVGEDPVKCQERAERCRRRRDQMRLRQSLINLIHGLQDCEFPPLGTHARQRLLKRILLKGRGCLRKLDHGYVRDLFREMEDLHMFAMKFAAVRHKTLTLNGTPYAFLTPEKLQSRFGSLFVPLLPSAMKEKGCEPVSGMQTLSRTRALLRRSPHFKKGITKAFKLAQFIQHQQDHCADVQDVLEEHWREYIYNETMATLGALEGEYHFYVDDMEKYLRSDLRKILRKIDIILSHHMMEFVRTSIADWKHFFLSFVADETQRPADPLLILDVRATENEKELLPFLQLPNPPLYEISATYEPLNETREAVQSVLRTCLGKAENYIALFNKFAYLSSDVAEGAKNAHVDFDPSDLPSVRERLEKYHTAMFEIETVCPTEVKLQMIVLDCSHVKETLSAKAEKLWKAECERVSKFIEDEIAAVVREWNDLHDRVLTIPTTEEELGSLKNLIATINELSDPLTSRTRFIHEAIDLLASVNFAINKTVHEEAYRAFSWPLQIKFDLVETGGILEKGRLRFEEKLEADKAELEKDLSQLEQEVDFVRNEMGDTEQAMEYYKRIVQLQEQLTAAKNRTEAFKKAETLFGLEEPSDYSRVDDLYAAFEPFRKLWALVVDFKYGELQWLSAAITSLDPAEVETSLDRWSQEAYKLRKHFLTDSRSVQVGVADALHLSIERFRQHLPVIRPLCNEAFLPRHWVDLLKTIGCDLDVDEGFSLTQLLDSGIQDHLQIVEEKSELAQKEFTLKNALVKMKVEWKAMQLQLIPFKDTHTYVMKGFDVVQALLDDHLVRTQTMRGSRFIRCIEYQSREWESKLLETQQALDSLQACQRSWMYLQPIFQSADISQQIPHEAGLFREVDDLWRRTVAQAEETPGVLDIVEFGNLHHDFTQANTKLAQIMRGVNDFLETKRLAFPRFFFLSNEELLAILSQTTDLSPVQMYLNKCFEGIHRVAFDEHGTVINAIRSAAGEEIPLGRHVALVDGSRFVGAEHWLGEVESSVSDSLRDVMEAAMKDYQIRPRTEWCLSWPGQVALTASQIFWTSEVADAINGAQLASYLDKCNDQLQGLVRLVRGRLTQLNRLTVTTLLTIDVHARDVVEELKKENTSSLSDFEWLAQLRLSWQQPGSVTLLTGKRNTKPEIQLSMINARIFYGFEYLGSTERLVITPLTDRCYRTLIGAFHLQYGGAPEGPAGTGKTETTKDLAKAAGMQCLVFNCSDGLDYVSIGRFFKGLVSSGAWCCFDEFNRIEVEVLSVIAQQVHAIQQAIRNQVKVFLFEGTDVKLVPSCAINITMNPGYAGRSDLPDNLKALFRPCAMMIPDFSLIAEVMLYSYGFADARNVARKALACLRLSSEQLSSQDHYDFGMRALKAILTAAGQLKRTFGSAFEEDELTLYALERVSVPKLTPNDTQLFRDIVSDLFPGVKVASEDNGFLLSRIEQVAKKKNLQPTEAFLTKCIQLWDTIKVRHGLMIVGATLTGKTAVLECLAEALGRACEGRERERENGGSDREDLFRDTKERHEGRKRRDRQRNAARQPLEADEGRVAVAQEISDEDEWEEEPNGDETEEEGREGAQQEKDEGGRRRGKEEGENGTVPHEYLPCKLYKINPKSVTQGQLYGKYDENTQEWTDGVLAIAIRAASNESDVQARQWIVLDGPVDAVWIENMNTVLDDNKRLCLTSGEIIKLSALTTMIFEVDDLSSASPATVSRCGMVYLDQAQCTWEPMVISWIHQQRQKAPNSLPLSCIDESESGLSLTDLFLHALPIFLQFIKVNSTTPCVVSDNW

Target ORF information:

RefSeq Version	XM_018781690.1
Organism	Toxoplasma gondii ME49
Definition	Toxoplasma gondii ME49 dynein heavy chain, n-terminal region 2 protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018781690.1

ORF Insert Sequence:

ATGGAAGAGC GCAGGGCATC CTCGACAAAT CACAGCGAGT GCCCTTCGGC TGCAGCAATT 
GAGTACAACA AACCACTTTT CTTGTTTTTA GATGGGCCGC TTAAGCTGAG TCTCGAAGAT 
TTCGACGATC CAGACCTGTA CGCAGATCCC TCTCCGCAGG AATGGGTCCG TCGCTGTGCT 
AGCCGGCGAA ACAAGGAGGC TCTGTGTCGG CTGGGACATT CTGCCAGTAA CGAGGCCTCA 
GCTCACTCTT GCATCTCTGC TACTCACTCA CGCCGCGGTT CTGTCAATGG CAAGCTAGAC 
GATAACAACG ACGGGAGGAA CACTAGTGGT GATGACCGTG CTCCGCGCTC GGGCTCCGAG 
GGCGGTTCTG ACACTCTCTC TGTCAGAAAT GGGGCTCCGC ACGAAACGGA CGAAAATTTG 
CCGAAAAGGG TGGTAGGACT GTCTGACGGT GTCTCAATCA TGGAAAGACA CACGTCGGAT 
GCAGTCTGCG AACACCTACA GTTTGACGAA CGGTGTTCGA AGGATGCAGA CGTGCTACAT 
TTCCTCGATG GCGACTGGCG GATGACCCCT TGTTGGGTTA CAGACTATTT CAAAGACGAA 
AAGAAATTCA AAGTGCGGTT GAAAGTGGAC GGGACCGAGA AAACCGTGCA TCGCCTCGCG 
ATCCGATTCG TTGGTGAAGA TCCAGTAAAG TGTCAAGAAA GAGCAGAACG ATGCCGACGC 
AGGCGGGACC AGATGCGTCT GCGCCAGTCT CTTATCAACC TCATTCACGG TCTCCAGGAC 
TGTGAGTTCC CACCGCTAGG AACACACGCC AGACAGCGAT TGCTGAAGCG CATTTTGCTC 
AAGGGTCGAG GCTGTCTGCG AAAGCTCGAT CATGGATATG TCCGTGACTT GTTCCGTGAG 
ATGGAAGACC TTCATATGTT CGCCATGAAG TTCGCAGCAG TCCGACACAA AACGCTTACC 
CTGAATGGTA CTCCCTACGC GTTTCTCACT CCTGAGAAGC TGCAGTCGAG ATTTGGATCT 
CTCTTCGTTC CTCTCCTTCC TTCGGCGATG AAGGAAAAGG GATGCGAACC TGTCTCCGGC 
ATGCAGACAC TCTCGAGAAC CCGGGCGCTG CTCAGGCGGA GTCCACATTT CAAGAAGGGA 
ATCACTAAGG CTTTCAAACT GGCTCAGTTC ATTCAACACC AGCAAGACCA CTGCGCAGAT 
GTTCAGGATG TCCTTGAAGA ACATTGGCGT GAATATATTT ACAATGAGAC CATGGCGACT 
CTTGGCGCAT TAGAAGGCGA ATACCATTTC TACGTCGACG ACATGGAGAA ATACTTGCGG 
AGCGACCTGC GCAAGATTCT GCGTAAAATT GACATCATCC TCTCACATCA CATGATGGAA 
TTCGTTCGGA CCTCGATTGC CGACTGGAAA CACTTCTTCC TCTCTTTCGT CGCCGACGAA 
ACACAACGCC CCGCTGACCC TCTCCTCATT CTTGATGTCC GCGCCACAGA AAATGAGAAG 
GAGCTGCTTC CCTTTCTTCA GTTGCCAAAT CCACCATTGT ACGAGATTTC GGCGACGTAT 
GAACCGCTGA ATGAGACTCG AGAGGCAGTC CAAAGCGTGC TGCGAACGTG TCTAGGAAAG 
GCAGAAAACT ACATCGCTCT CTTCAACAAG TTTGCATATT TATCCAGTGA TGTTGCAGAG 
GGCGCAAAGA ACGCTCACGT CGACTTTGAT CCGTCAGACC TTCCGAGTGT CCGCGAACGT 
CTAGAGAAAT ACCACACTGC AATGTTTGAA ATTGAGACGG TCTGCCCGAC AGAAGTCAAA 
CTGCAGATGA TCGTACTGGA TTGCTCGCAC GTGAAAGAAA CACTTTCGGC CAAGGCGGAA 
AAGCTCTGGA AAGCTGAATG CGAGAGAGTG TCTAAGTTCA TAGAGGATGA AATAGCTGCT 
GTCGTGAGAG AGTGGAACGA CCTCCATGAC CGCGTCTTGA CTATTCCAAC GACAGAGGAA 
GAGCTAGGTT CCTTAAAAAA CCTCATCGCC ACAATAAATG AATTGTCGGA TCCCCTGACG 
TCAAGAACGA GGTTCATTCA TGAAGCCATC GACCTACTCG CGTCGGTCAA TTTCGCCATC 
AACAAAACTG TGCACGAAGA GGCCTACAGG GCCTTCAGCT GGCCTCTCCA GATTAAATTC 
GACCTTGTGG AAACGGGTGG AATTCTTGAA AAAGGACGAC TGCGGTTTGA GGAGAAATTG 
GAAGCGGACA AGGCAGAGTT GGAGAAGGAC TTGAGCCAGC TGGAGCAAGA AGTTGATTTC 
GTCCGAAACG AGATGGGCGA CACCGAGCAG GCGATGGAAT ATTACAAACG AATCGTGCAG 
CTGCAGGAAC AACTGACGGC CGCCAAGAAC CGAACTGAGG CCTTTAAGAA GGCAGAAACT 
CTGTTTGGCT TGGAGGAACC GTCCGACTAC AGTCGTGTCG ACGACCTTTA TGCAGCCTTC 
GAGCCTTTCA GAAAATTGTG GGCGTTGGTC GTCGACTTCA AGTACGGTGA ACTTCAGTGG 
CTGAGCGCCG CCATCACGTC TTTGGATCCG GCGGAGGTCG AGACGAGTTT GGATCGATGG 
AGTCAAGAGG CGTACAAGTT GCGGAAGCAC TTTCTCACAG ATTCACGTTC CGTGCAGGTC 
GGAGTCGCGG ATGCGTTGCA CCTCTCCATA GAACGCTTTC GTCAGCATCT TCCTGTGATT 
CGTCCTCTCT GCAACGAGGC GTTTTTACCG CGGCACTGGG TCGATCTGCT GAAAACCATT 
GGTTGCGACC TAGACGTCGA CGAGGGCTTC TCCCTCACTC AACTTCTCGA CAGCGGGATC 
CAAGACCATC TACAGATCGT CGAAGAAAAA AGCGAGCTCG CCCAGAAGGA GTTCACGTTG 
AAGAACGCCC TGGTGAAGAT GAAGGTCGAG TGGAAAGCGA TGCAGCTCCA ACTCATCCCT 
TTCAAAGACA CACACACGTA CGTCATGAAA GGCTTTGATG TCGTCCAGGC TCTTCTGGAC 
GACCACCTCG TACGCACGCA GACGATGCGC GGCTCGCGCT TCATCCGGTG CATAGAATAC 
CAAAGTCGAG AGTGGGAATC GAAGTTGCTG GAAACCCAAC AGGCTCTCGA CAGTCTCCAA 
GCCTGCCAAC GTTCCTGGAT GTACCTCCAG CCGATTTTCC AGAGCGCAGA CATCTCGCAG 
CAAATTCCAC ACGAGGCAGG GCTGTTCCGA GAGGTCGATG ACCTGTGGAG ACGCACCGTC 
GCCCAAGCCG AAGAGACACC CGGAGTTCTG GATATCGTGG AATTCGGCAA TCTCCACCAC 
GACTTCACGC AAGCAAATAC GAAGCTAGCG CAGATTATGA GGGGCGTGAA CGACTTCCTC 
GAGACCAAGC GCCTCGCCTT CCCCAGATTC TTCTTCCTCT CCAACGAGGA GCTCCTCGCG 
ATCCTGTCGC AAACGACGGA CCTGTCACCC GTTCAAATGT ATTTGAACAA ATGCTTCGAG 
GGGATTCACA GAGTCGCTTT CGACGAACAT GGGACGGTCA TAAACGCCAT ACGAAGCGCT 
GCCGGAGAAG AAATTCCTCT CGGACGCCAC GTGGCTCTCG TGGACGGATC CCGATTTGTC 
GGAGCTGAAC ACTGGCTTGG CGAGGTTGAA AGCTCTGTCA GTGACAGCCT GCGGGATGTC 
ATGGAAGCTG CGATGAAAGA TTACCAGATA CGACCCAGAA CAGAATGGTG TTTATCCTGG 
CCCGGTCAGG TCGCCCTAAC GGCCAGCCAG ATCTTCTGGA CTTCAGAGGT CGCGGACGCG 
ATCAATGGTG CCCAACTAGC TAGCTACCTG GACAAATGCA ATGATCAACT TCAGGGACTC 
GTTCGTCTGG TCCGCGGCCG CTTAACGCAG CTGAATCGCC TGACAGTGAC GACTCTTCTG 
ACCATCGACG TGCATGCGCG GGACGTGGTG GAAGAGTTGA AGAAGGAGAA CACGTCTTCT 
TTGTCGGATT TCGAGTGGCT CGCGCAATTG CGTCTGTCGT GGCAGCAGCC AGGAAGTGTC 
ACTTTGCTGA CGGGAAAGAG AAACACAAAG CCGGAGATTC AGCTGAGCAT GATCAACGCG 
CGAATCTTTT ACGGATTTGA ATACTTGGGC AGCACGGAAA GACTCGTCAT CACGCCCCTA 
ACTGACCGGT GCTACCGAAC TCTGATCGGC GCTTTCCACT TGCAGTACGG CGGCGCACCG 
GAAGGTCCAG CGGGGACAGG AAAAACTGAA ACGACCAAAG ACTTGGCAAA AGCTGCGGGT 
ATGCAGTGTC TCGTTTTCAA CTGCTCTGAC GGTCTCGACT ACGTGTCGAT TGGGCGCTTC 
TTCAAGGGCC TGGTTTCCTC GGGAGCTTGG TGCTGCTTCG ACGAATTCAA CCGAATCGAA 
GTCGAAGTTC TGTCGGTCAT TGCTCAACAA GTGCATGCAA TTCAACAGGC CATACGCAAC 
CAGGTTAAAG TTTTTCTTTT TGAAGGGACG GATGTGAAAC TCGTACCCTC GTGCGCCATC 
AATATTACCA TGAATCCGGG CTATGCCGGA AGATCCGATC TACCAGACAA CCTGAAGGCT 
CTCTTCCGAC CTTGCGCGAT GATGATTCCG GACTTTTCGT TGATTGCGGA AGTGATGCTA 
TACTCATATG GATTTGCAGA CGCCCGGAAT GTGGCTCGCA AGGCCCTGGC GTGCCTCCGT 
CTGTCGTCAG AGCAGCTGTC ATCGCAAGAC CACTACGACT TTGGTATGCG CGCTCTGAAG 
GCGATTCTTA CCGCAGCCGG GCAGTTGAAA AGAACGTTTG GATCAGCGTT TGAGGAAGAC 
GAACTGACCC TGTACGCGCT GGAGAGGGTC AGCGTTCCCA AGCTTACTCC CAACGATACT 
CAGCTCTTCC GTGACATCGT TTCAGATCTG TTTCCAGGGG TGAAAGTGGC CTCTGAAGAC 
AACGGCTTTC TGCTTTCTCG TATCGAGCAG GTTGCGAAGA AAAAGAACCT TCAACCTACA 
GAGGCGTTTC TAACAAAGTG CATTCAACTC TGGGACACCA TCAAAGTGAG GCACGGGCTC 
ATGATTGTGG GGGCGACACT GACGGGGAAA ACGGCGGTGC TGGAGTGTCT CGCGGAGGCT 
CTTGGACGCG CCTGCGAAGG CCGAGAACGC GAACGGGAAA ACGGAGGCAG TGATCGCGAA 
GATCTATTCA GAGACACCAA AGAGAGACAC GAAGGGAGGA AAAGGAGAGA CAGGCAACGC 
AACGCTGCCA GGCAGCCGTT GGAGGCCGAC GAAGGCCGAG TTGCAGTAGC GCAAGAGATA 
AGCGACGAAG ACGAATGGGA GGAAGAACCC AACGGAGACG AAACAGAAGA AGAAGGTCGA 
GAGGGAGCCC AGCAGGAAAA AGACGAAGGA GGTCGCCGAC GTGGAAAGGA AGAGGGGGAA 
AATGGGACAG TACCTCACGA ATACTTGCCA TGCAAACTGT ATAAAATCAA TCCGAAAAGT 
GTTACACAGG GACAGCTTTA CGGCAAATAC GATGAAAACA CGCAAGAGTG GACAGACGGA 
GTTCTTGCAA TCGCTATTCG GGCTGCCTCC AACGAAAGCG ATGTTCAAGC CCGGCAGTGG 
ATCGTCCTTG ACGGCCCCGT CGATGCGGTG TGGATTGAGA ACATGAATAC GGTCTTGGAC 
GACAACAAAA GGCTTTGTCT CACGAGCGGA GAGATCATCA AACTCTCGGC CCTGACTACG 
ATGATCTTCG AAGTCGATGA CCTCTCCTCT GCTTCTCCAG CCACTGTCTC CCGTTGCGGG 
ATGGTCTACC TTGATCAAGC GCAGTGCACT TGGGAGCCGA TGGTGATTTC CTGGATCCAT 
CAGCAGAGGC AGAAAGCACC AAACTCCCTT CCGCTGTCCT GCATAGACGA AAGCGAGTCC 
GGGTTGTCTC TCACAGACTT GTTTCTCCAT GCTCTCCCGA TCTTCCTTCA ATTTATAAAG 
GTCAACAGTA CAACGCCTTG CGTCGTGTCA GATAACTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TGME49_273478 cDNA ORF clone, Toxoplasma gondii ME49

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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