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random activation of gene expression (RAGE)

A procedure for creating a genome-wide library of cDNAs by use of several insertion vectors, each containing a promoter sequence linked to an exon and followed by an unpaired splice site. transfection into eukaryotic cells allows the vectors to randomly insert by non-homologous recombination. Where the insertion is upstream from a host cell gene, it activates expression of a transcript that encodes a chimeric protein that containing the vector-encoded sequence and some or all of the host cell protein.Harrington, J.J., Sherf, B., Rundlett, S. et al. (2001) Nat. Biotechnol. 19, 440-445

Related Biology Tools

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    GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.

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    GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.

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Service and Products

Protein Expression

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Insect Expression

GenScript's BacuVance baculovirus expression system was developed by our in-house team of scientists for virus production and expression of recombinant proteins from baculovirus-infected insect cells.

Mammalian Transient Expression

Proprietary High Density (HD) expression system, enhance the protein yield up to 100 fold, achieve antibody titers up to 3 g/L.

Reagents for COVID-19 Research

Gram level, ready to ship RBD proteins, ACE2 assay cell lines and pseudovirus.

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