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This tool calculates melting temperature (Tm), molecular weight (MW), GC content, Extinction Coeff (L/(mole·cm)), nmol/OD260, ug/OD260 for oligos

Please right click in "sequence tab" to insert internal modification.

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For DNA type:
Standard DNA Bases (A, C, G, T)
Mixed Bases, expressed in UPPERCASE.
Locked nucleic acid bases (LNA) are entered as '+_'.
Phosphorothioated DNA bases are entered as '_*'
Phosphorothioated LNA bases are entered as '+_*'.
For RNA type:
Standard RNA Bases (A, C, G, U)
Mixed Bases, expressed in UPPERCASE.
Chimeric DNA base are entered as 'd_'.
2' O-methyl RNA bases are entered as 'm_'.
Locked nucleic acid bases (LNA) are entered as '+_'.
Phosphorothioated RNA bases are entered as '_*'.
Phosphorothioated 2'-O-methyl RNA bases are entered as 'm_*'.
Phosphorothioated LNA bases are entered as '+_*'.