Strategies to Efficiently Generate CRISPR KO/KI Cell Lines

Dr. Zhou obtained his Ph.D degree in Molecular Biology and Genetics from Chinese Academy of Science (CAS) in 2012, focused on studying the mechanism of how steroid hormone regulates IIS-TORC1 signaling in insect fat body, using molecular and genetics tools and techniques, including DNA cloning, gene overexpression and RNAi, and Drosophila genetic manipulation. Dr. Zhou then did two years of postdoc training in BioMedical Engineering at Tufts University, using molecular method to engineer recombinant spider silk to facilitate osteogenesis in vitro. Since 2014, Dr. Zhou works as a senior scientist at GenScript, and he has established a CRISPR-based genome editing cell line engineering platform, with which he has delivered 500+ CRISPR cell lines. Dr. Zhou has plenty of hands-on experience on CRISPR design.