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Characterization of the complete mitochondrial genome of Dnopherula yuanmowensis (Orthoptera: Acrididae) and its phylogenetic implications

Journal Mitochondrial DNA Part B. 2019;

Ran Li, Wei-An Deng, Xiao-Han Shu, Hao-Wu Hu &Xiao-Dong Li

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Gene Synthesis	Certain pairs of universal primers for grasshopper mitochondrial genomes were used for polymerase chain reaction (PCR) amplification (Simon et al. 2006). Then PCR products were sequenced using primer-walking strategy from both strands by Genscript Biotech Corp. (Nanjing, China). Mitochondrial genome was assembled by SeqMan program from DNASTAR (Burland 2000), and annotated using MITOS Web Server (Bernt et al. 2013).	Get A Quote

Abstract

The complete mitochondrial genome (mitogenome) of Dnopherula yuanmowensiswas sequenced and characterized in our study. The mitogenome is a circular molecule of 15,629 bp in length, containing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and an A + T-rich region, as in other grasshoppers. The AT content of the overall base composition is 74.9%. Phylogenetic reconstruction using both Bayesian Inference (BI) and Maximum Likelihood (ML) validated the taxonomic status of D. yuanmowensis, placing it in the monophyletic Gomphocerinae.

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