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Gene Synthesis> | Conidia dimensions were 28 to 54 × 4.5 to 6 µm, and beaks when present were 2 to 4 µm (Kou et al. 2014). Based on the morphological characters, the fungus was tentatively identified as Alternaria species (Simmons 2007; Woudenberg et al. 2015). Fungal genomic DNA was extracted from two representative isolates using a Qiagen kit followed by PCR amplification with internal transcribed spacer (ITS) ITS 1/ITS 4 primers. The amplified PCR products were cleaned and sent for Sanger sequencing by GenScript (Piscataway, NJ). Isolates 1422F (GenBank accession MK611645) and 1424F (MK611649) had 98 and 100% sequence identity with Alternaria tenuissima (GenBank accessions MF373440 and MH374277), respectively (Ziedan et al. 2018). | Get A Quote |