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Proteins, Expression, Isolation and Analysis> | … The gels were destained in a solution containing 10% v/v acetic acid and 10% methanol. Molecular weight marker (30–150 kDa) was used to compute molecular weight (GenScript Corp, Piscataway, NJ). Statistical Analysis. All experiments were carried out in triplicate … | Get A Quote |
The effect of different drying methods used in the preparation of protein concentrates from different cultivars of cowpea and bambara bean was investigated. Protein was extracted by the isoelectric precipitation method and subsequently dried by cabinet drying (CD), vacuum drying (VD) and freeze drying (FD). The protein extraction yield and protein content were high. The subunit composition of protein concentrates was characterized by electrophoresis. The drying methods affected water‐holding capacity, oil absorption capacity and solubility of protein concentrates depending on cultivars. Water‐holding capacity was adequate for its utilization in viscous foods. The protein concentrates prepared by FD showed h... More