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Application of nucleic acid aptamers for detection of Apple stem pitting virus isolates.

Mol Cell Probes.. 2017-10; 
Komorowska B, Hasiów-Jaroszewska B, Minicka J.
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Proteins, Expression, Isolation and Analysis ... kit (Machery-Nagel). Plant extracts after denaturation were resolved using SDS-PAGE, and then transferred onto a nitrocellulose membrane by semi-dry blotting using eBlot™ Protein Transfer (GenScript). First, the membranes ... Get A Quote

Abstract

DNA aptamers (PSA-H and MT32) were applied for the detection of Apple stem pitting virus (ASPV) isolates using an Enzyme-Linked Oligonucleotide Assay (ELONA) and Western blot analysis. The specificity and effectiveness of aptamers were verified in comparison to a conventional Enzyme Linked Immunosorbent Assay (ELISA). A genetically diverse group of ASPV isolates was tested. The results showed that aptamer MT32 detected a wider range of ASPV isolates than an aptamer PSA-H and proved to be superior to commercially available monoclonal antibodies. Aptamer MT32 produced higher signal intensity in ELONA with a virus-infected plant extracts than antibodies in ELISA. Moreover, the ELISA method failed to detect ASPV in... More

Keywords

ASPV; Aptamers; Detection; ELONA; Western blot
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