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B7-2/CD86, His, Human

Recombinant Human B7-2/CD86 produced in HEK293 cells is a polypeptide chain containing 228 amino acids with C-terminal 6His. A fully biologically active molecule, rhB7-2/CD86 has a molecular mass of 45-55 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
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Product Introduction
Species Human
Protein Construction
B7-2/CD86 (Leu20-Pro247)
Accession # P42081		 Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.1 EU/μg of protein by gel clotting method
Biological Activity Assay #1: Immobilized B7-2 His, Human at 5.0 μg/ml (100 µl/well) can bind CTLA-4 Fc, Human.Assay #2: Immobilized B7-2 His, Human at 5.0 μg/ml (100 µl/well) can bind CD28 Fc, Human.
Expression System HEK 293
Apparent Molecular Weight 45~55 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. B7-2 promotes the stabilization of CD28 in the IS, while The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response. Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses
Synonyms Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD86; B7-2; CD28LG2; LAB72; MGC34413

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.