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B7-H3 Fc Chimera, Human

Recombinant Human B7-H3 produced in HEK293 cells is a polypeptide chain containing 450 amino acids with C-terminal human IgG1 Fc fragment. A fully biologically active molecule, rhB7-H3 has a molecular mass of 62-65 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03426
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Product Introduction
Species Human
Protein Construction
B7-H3 (Leu29-Pro245)
Accession # Q5ZPR3-2		 hFc
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Expression System HEK 293
Apparent Molecular Weight 62~65 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Human B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Human B7-H3 does not bind any known members of the CD28 family of immunoreceptor. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to co-stimulate the proliferation of CD4+ or CD8+ T cells. B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production.
Synonyms B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7; CD276ss

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.