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SIRPα, His, Human

Immobilized SIRPa-His, Human(Cat.No.Z03418) at 2 μg/mL (100 µl/well), can bind CD47 Fc Chimera, Human (Z03418) with a linear range of 0.25-185 ng/mL.

SIRPα, His, Human

Recombinant Human SIRPα produced in HEK293 cells is a polypeptide chain containing 346 amino acids with C-terminal 6His. A fully biologically active molecule, rhSIRPα has a molecular mass of 50-55 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03421
$275.00

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Product Introduction
Species Human
Protein Construction
SIRPα (Glu31-Arg370)
Accession # P78324		 Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity Immobilized SIRPa-His, Human at 2.0 μg/ml (100 µl/well), can bind CD47 Fc Chimera, Human (Cat. No.: Z03418) with a linear range of 0.25-185.0 ng/ml.
Expression System HEK 293
Apparent Molecular Weight 50~55 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, 5% trehalose and mannitol.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • SIRPα, His, Human
    • SIRPα, His, Human

    Immobilized SIRPa-His, Human(Cat.No.Z03418) at 2 μg/mL (100 µl/well), can bind CD47 Fc Chimera, Human (Z03418) with a linear range of 0.25-185 ng/mL.


Background
Target Background Signal regulatory protein alpha (SIRPα, designated CD172a), is also known as CD172 antigen-like family member A (CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), which is a monomeric about 90kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily. SIRPα is Ubiquitous and highly expressed in brain. SIRPA/CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA/SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function by similarity. SIRPα recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production.
Synonyms CD172 antigen-like family member A; CD172a antigen; CD172a

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.