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CD28 Fc Chimera, Human

Immobilized human CD28 Fcat 2μg/mL (100μL/well) can bind human Biotin-B7-1(CD80) Fc with a linear range of 0.01-0.5μg/mL.

CD28 Fc Chimera, Human

Recombinant Human CD28 Fc Chimera produced in HEK293 cells is a polypeptide chain containing 363 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhCD28 has a molecular mass of 66-70 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03413
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Product Introduction
Species Human
Protein Construction
CD28 (Asn19-Pro152)
Accession # P10747		 hFc
N-term C-term
Purity > 97% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity Immobilized CD28, hFc, Human at 2.0 μg/ml (100 µl/well) can bind human Biotin-B7-1(CD80) Fc when detected by Streptavidin-HRP second antibody.
Expression System HEK 293
Apparent Molecular Weight 66~70 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • CD28 Fc Chimera, Human
    • CD28 Fc Chimera, Human

    Immobilized human CD28 Fcat 2μg/mL (100μL/well) can bind human Biotin-B7-1(CD80) Fc with a linear range of 0.01-0.5μg/mL.


Background
Target Background Human CD28 is composed of four exons encoding a protein of 220 amino acids that is expressed on the cell surface as a glycosylated, disulfide-linked homodimer of 44 kDa. Members of the CD28 family share a number of common features. These receptors consist of paired V-set immunoglobulin superfamily (IgSF) domains attached to single transmembrane domains and cytoplasmic domains that contain critical signaling motifs. The CD28 and CTLA4 ligands, CD80 and CD86, consist of single V-set and C1-set IgSF domains. The interaction of these costimulatory receptors with ligands is mediated through the MYPPPY motif within the receptor V-set domains. CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 costimulation has diverse effects on T cell function, including biochemical events at the immunological synapse, downstream phosphorylation and other post-translational modifications, transcriptional changes, and cytoskeletal remodeling. At the most basic level, CD28 signals increase a cell’s glycolytic rate, allowing cells to generate the energy necessary for growth and proliferation.
Synonyms CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.