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CD19 Fc Chimera, Human

Recombinant Human CD19 Fc Chimera produced in CHO cells is a polypeptide chain containing 505 amino acids with the C-terminal human IgG1 Fc fragment. A fully biologically active molecule, rhCD19 a molecular mass of 62 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03407
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Product Introduction
Species Human
Protein Construction
CD19 (Pro20-Lys291)
Accession # P15391		 hFc
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Expression System CHO
Apparent Molecular Weight ~62 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells. On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor. Mature human CD19 consists of a 272aa extracellular domain (ECD) with two Ig-like domains, a 22aa transmembrane segment, and a 243aa cytoplasmic domain.
Synonyms CD19; Leu-12; B4; B-lymphocyte antigen

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.