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CTLA-4 Fc Chimera, Human

GenScript product showed equal activity compared to competitor A when binding with biotin CD80 Fc,Human.

CTLA-4 Fc Chimera, Human

Recombinant CTLA-4, hFc, Human produced in CHO cells is a polypeptide chain containing 378 amino acids with the C-termimal human IgG1 Fc fragment. A fully biologically active molecule, rhCTLA-4 has a molecular mass of 45-48 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03373
$65.00

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Product Introduction
Species Human
Protein Construction
CTLA-4 (Ala37-Phe162)
Accession # P16410		 hFc
N-term C-term
Purity > 98% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity Assay #1: Measured by its ability to inhibit IL-2 secretion by co-culturing stimulated Jurkat human acute T cell leukemia cells and CD80 expression CHO stable cell line.
Assay #2: Immobilized B7-2(CD86), His, Human (Cat. No.: Z03452) at 2.0 μg/ml (100 µl/well) can bind CTLA-4, hFc, Human.
Expression System CHO
Apparent Molecular Weight 45~48 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • CTLA-4 Fc Chimera, Human
    • CTLA-4 Fc Chimera, Human

    GenScript product showed equal activity compared to competitor A when binding with biotin CD80 Fc,Human.


Background
Target Background Cytotoxic T lymphocyte-associated molecule-4 (CTLA-4) is a cell surface molecule that is closely related to CD28, and a powerful negative regulator of T cell activation. Structurally, CTLA-4 is a member of the Ig superfamily, having a single extracellular V-like domain , homology with CD28; The overall sequence homology between CD28 and CTLA-4 is about 20%, but they share a 27% (murine) to 31% (human) identity at the amino acid level. Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA-4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Synonyms CTLA-4

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.