Catalog Products » Recombinant Proteins » MDC/CCL22 (69aa), Human

MDC/CCL22 (69aa), Human

Recombinant Human MDC/CCL22 (69aa) produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMDC (69aa) has a molecular mass of 8.1 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03335
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Product Introduction
Species Human
Protein Construction

Expressed with an N-terminal Met.

CCL22 (Gly25-Gln93)
Accession # O00626-1
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity The EC50 value of human MDC/CCL22(69aa)on Ca2+ mobilization assay in CHO-K1/Gα15/hCCR4 cells (human Gα15 and human CCR4 stably expressed in CHO-K1 cells) is less than 1.0 μg/ml.
Expression System E. coli
Apparent Molecular Weight ~8.1 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Macrophage-Derived/CCL22 Chemokine (MDC) , also known as stimulated T cell chemotactic protein (STCP­1), is a CC chemokine initially isolated from clones of monocyte­derived macrophages. CCL22 is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. CCL22 shows chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. CCL22 has mild chemotactic activity for primary activated T lymphocytes and no chemoattractant activity for neutrophils, eosinophils or resting T lymphocytes. CCL22 may also be involved in certain aspects of activated T lymphocyte physiology, such astrafficking activated T lymphocytes to inflammatory sites. CCL22 interacts with the cell surface chemokine receptor CCR4.
Synonyms C-C motif chemokine 22; Small-inducible cytokine A22; Macrophage-derived chemokine; MDC(1-69); Stimulated T-cell chemotactic protein 1; CC chemokine STCP-1; CCL-22; MDC

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.