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M-CSF, Rat

Recombinant Rat Macrophage Colony Stimulating Factor (M-CSF) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 155 amino acids each. A fully biologically active molecule, rrM-CSF has a molecular mass of 28 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03301
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Product Introduction
Species Rat
Protein Construction

Expressed with an N-terminal Met.

M-CSF (Glu33-Pro186)
Accession # Q8JZQ0-1
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 5.0 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 2.0 × 105 units/mg.
Expression System E. coli
Apparent Molecular Weight ~28 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. In mammals, it exits three isoforms, which invariably share an N-terminal 32-aa signal peptide, a 149-residue growth factor domain, a 21-residue transmembrane region and a 37-aa cytoplasmictail. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers. The biological activity of human M-CSF is maintained within the 149-aa growth factor domain, and it is only active in the disulfide-linked dimeric form, which is bonded at Cys63.
Synonyms CSF1; CSF-1; MCSF; colony stimulating factor 1; Macrophage Colony Stimulating Factor; Lanimostim; MGC31930; MGI-IM

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.