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MIP-3α/CCL20, Human

Recombinant Human MIP-3α/CCL20 produced in E.coliis a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhMIP-3α/CCL20has a molecular mass of 8.0 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Z03272
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Product Introduction
Species Human
Protein Construction
MIP-3α (Ala27-Met96)
Accession # P78556
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity The EC50 value of humanMIP-3 alpha/CCL20 on Ca2+ mobilization assay in CHO-K1/G15/hCCR6 cells (human Ga15 and human CCR6 stably expressed in CHO-K1 cells) is less than 200.0 ng/ml.
Expression System E. coli
Apparent Molecular Weight ~8 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against 53 mM Na2HPO4, 147 mM NaH2PO4, 300 mM NaCl, pH 6.5.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Macrophage Inflammatory Protein-3 (MIP-3α), also known as chemokine (C-C motif) ligand 20 (CCL20) or liver activation regulated chemokine (LARC), is a small cytokine belonging to the CC chemokine family. MIP-3α is expressed in the liver, lymph nodes, appendix, PBL and lung and can signal through the CCR6 receptor. It is strongly chemotactic for lymphocytes and weakly attracts neutrophils. MIP-3α is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells toward the epithelial cells surrounding these tissues. Additionally, it promotes the adhesion of memory CD4+ T cells and inhibits colony formation of bone marrow myeloid immature progenitors.
Synonyms CCL-20; CKb4; Exodus; LARC; MIP-3-alpha; MIP-3a; MIP3A; SCYA20; ST38; chemokine (C-C motif) ligand 20; C-C motif chemokine ligand 20

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.