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IGF-I, Human

ED₅₀< 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0× 10⁵ units/mg.

IGF-I, Human

GenScript product showed better activity compared to competitor S

IGF-I, Human

Recombinant human Insulin-like growth factor I (rhIGF-I) produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhIGF-I has a molecular mass of 7.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Z03017
$45.00

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Product Introduction
Species Human
Protein Construction
IGF-I (Gly49-Ala118)
Accession # P05019
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 5.0 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0 × 105 units/mg.
Expression System E. coli
Apparent Molecular Weight ~7.7 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • IGF-I, Human
    • IGF-I, Human

    ED₅₀< 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0× 10⁵ units/mg.

  • IGF-I, Human
    • IGF-I, Human

    GenScript product showed better activity compared to competitor S


Background
Target Background Insulin-like growth factor I (IGF-I) also known as Somatamedin C is a hormone similar in molecular structure to insulin. Human IGF-I has two isoforms (IGF-IA and IGF-IB) which are differentially expressed by various tissues. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively. Both IGF-I and IGF-II (another ligand of IGF) can signal through the IGF-I receptor (IGFIR), but only IGF-II can bind the IGF-II receptor (IGFIIR/ Mannose-6-phosphate receptor). IGF-I plays an important role in childhood growth and continues to have anabolic effects in adults.
Synonyms Somatomedin C; IGF-I; IGFI; IGF1; IGF-IA; Mechano growth factor; MGF; Insulin-like Growth Factor-I

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.