Catalog Products » Recombinant Proteins » Enzymes and Inhibitors » Enterokinase (EK), His, Bovine

Enterokinase, His, Bovine

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed to be removed from digestion system. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript. Components: 100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl 2, 50% glycerol) 100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0) 3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol) 1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl₂)
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Description

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.
Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.
Components:

  • 100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl,
    2mM CaCl 2, 50% glycerol)
  • 100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
  • 3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol)
  • 1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl₂)

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    Synonyms

    Enteropeptidase, ENTK, PRSS7

    Note

    For research use only

    Overview
    Key Features Components:
    100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
    100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
    3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
    1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2)
    Storage: Store kit components at –20°C
    Biological Activity 5 IU/μl.
    Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.
    Source P. pastoris
    Species Bovine

    Properties
    Molecular Weight ~40 kDa, observed by reducing SDS-PAGE.
    Purity > 95% by SDS-PAGE analyses.
    Endotoxin Level < 1.0 EU/μg, determined by LAL method.
    Formulation Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
    Storage & Stability Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity.Please avoid freeze-thaw cycles.