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FGF-4, Human

Recombinant human Fibroblast Growth Factor-4 (rhFGF-4) produced in E. coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule obtained by proprietary chromatographic techniques at GenScript, rhFGF-4 has a molecular mass of 19.4kDa as analyzed by reducing SDS-PAGE.
Z02984
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Product Introduction
Species Human
Protein Construction

Expressed with an N-terminal Met.

FGF-4 (Ala31-Leu206)
Accession # P08620
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity ED50 < 0.5 ng/ml, measured in a cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 2.0 × 106 units/mg.
Expression System E. coli
Apparent Molecular Weight ~19.4 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against 50 mM HEPES, 750 mM NaCl, pH 7.5.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Background
Target Background Fibroblast Growth Factor-4 (FGF-4) also known as K-FGF is a heparin-binding growth factor of the FGF family.It was identified by its oncogenic transforming activity. FGF-4 and FGF-3 are located closely on chromosome 11. FGF-4 and its receptors (FGF R1c, 2c, 3c and 4) play an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF-4 is required for normal limb and cardiac valve development during embryogenesis.
Synonyms HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.