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EGF, Human

Biological Activity
EGF, Human (Cat. No. Z00333) stimulates cell proliferation of BALB/c 3T3 cells. The ED50 for this effect is typically 5–100pg/mL.

EGF, Human

GenScript product showed equal activity compared to competitor P

EGF, Human

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Recombinant human Epidermal Growth Factor (EGF) is a 6.2 kDa protein containing 54 amino acid residues._x000D_
Z00333
$30.00

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Product Introduction
Species Human
Protein Construction

Expressed with an N-terminal Met.

EGF (Asn971-Arg1023)
Accession # P01133
Purity > 95% as analyzed by SDS-PAGE
> 95% as analyzed by SEC-HPLC
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of 5.0 × 106 IU/ mg.
Expression System E. coli
N-terminal Sequence Analysis The sequence of the first fifteen N-terminal amino acids was determined and found to be Met- Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys.
Apparent Molecular Weight ~6.2 kDa, on SDS-PAGE under reducing conditions.
Formulation Lyophilized after extensive dialysis against 10 mM phosphate buffer, pH 7.0, 200 mM NaCl buffer.
Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.

Examples
  • EGF, Human
    • EGF, Human

    Biological Activity
    EGF, Human (Cat. No. Z00333) stimulates cell proliferation of BALB/c 3T3 cells. The ED50 for this effect is typically 5–100pg/mL.

  • EGF, Human
    • EGF, Human

    GenScript product showed equal activity compared to competitor P


Background
Target Background Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through the EGF receptor (EGFR) also known as erbB1, is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). EGF-receptor binding results in cellular proliferation, differentiation, and survival. EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents.
Synonyms Urogastrone; URG; epidermal growth factor

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.