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Ovalbumin (OVA) mRNA (Cap1, m1Ψ)

RP-A00043
$300.00

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Description

Ovalbumin is the main protein found in egg white. It is a commonly used antigen for stimulating an allergic reaction in test subjects. It is also widely used in studies of serpin structure and function in biochemistry.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA 
- An integrated CD33 signal peptide to facilitate secretion of the expressed protein
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 1482nt
Full mRNA Molecular Weight 462888
Storage Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer 1mM Sodium citrate, pH6.5

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1-0.5 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96 well plate following manufacture’s instruction. Collect the cell supernatant after 12 to 48 hours, cell supertant was diluted 250x using PBS, and the ovalbumin secretion was measured using ELISA kit Ovalbumin (Sandwich ELISA) ELISA Kit - LS-F9540 from LS Bio.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.