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mCherry mRNA (Cap1, m1Ψ)

RP-A00041
$300.00

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Description

The mCherry mRNA expresses the fluorescent protein mCherry, which is a DsRed protein found in Discosoma Sp. mCherry protein has a maximum excitation wavelength at 587nm and maximum emission at 610nm. Due to the overlap of excitation and emission, using 560nm as excitation wavelength and 620nm as emission wavelength is recommended for your assay.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 974nt
Full mRNA Molecular Weight 318370
Storage Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer 1mM Sodium citrate, pH6.5

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1-0.5 ug of mRNA using 0.5 uL of Lipofectamine™ MessengerMAX™ Transfection Reagent (or equivalent) for one well of cells in a 96 well plate following manufacture’s instruction. Test the result after 12 to 48 hours using plate reader, or flow cytometry. Suggest using excitation wavelength at 560 nm, emission wavelength at 620 nm for equipment setting.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.