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F-Luc Self-Amplifying RNA (Cap1, m5C)

RP-A00032
$300.00

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Description

The Firefly-Luciferase (F-Luc) self-amplifying RNA (saRNA) expresses firefly luciferase protein, which is the enzyme for bioluminescence of fireflies and click beetles. After efficient delivery into cells or animals, F-Luc mRNA can translate into F-Luc and emit bioluminescence in the presence of its substrate, Luciferin.  
 
This saRNA features: 
- A Cap1-AU structure with high capping efficiency 
- 100% substitution with 5-methylcytidine (m5C) for improved protein expression and reduced innate immune response 
- Constructed using GenScript’s patented saRNA Vector 1 
- A 68A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 11044nt
Full mRNA Molecular Weight 3760097
Storage Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer 1mM Sodium citrate, pH6.5

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 70%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.01-0.05 µg of saRNA with 0.5 µL of Lipofectamine™ 2000 (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s protocol. Assess luciferase expression 12-48 hours post-transfection using luciferase assay system (Promega, E4030).

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.