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F-Luc mRNA (Cap1, m1Ψ)

RP-A00023
$300.00

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Description

The Firefly Luciferase (F-Luc) mRNA encodes the enzyme responsible for the bioluminescence seen in fireflies and click beetles. Upon delivery into cells or animals, it is translated into F-Luc protein, which emits light in the presence of its substrate, Luciferin.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- A 100A poly(A) tail to mimic natural mature mRNA 
 
This construct offers strong, reliable bioluminescent signaling, making it ideal for transfection optimization, biodistribution studies, and LNP delivery validation.
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 1909nt
Full mRNA Molecular Weight 622225
Storage Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer 1mM Sodium citrate, pH6.5

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.1-0.5 µg of mRNA with 0.5 µL of Lipofectamine™ MessengerMAX™ (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s instructions. Assess luciferase expression 12-48 hours post-transfection using luciferase assay system (Promega, E4030).

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.