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eGFP Self-Amplifying RNA (Cap1, m5C)

RP-A00015
$300.00

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Description

The EGFP self-amplifying RNA (saRNA) expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP saRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm.  
 
This saRNA features: 
- A Cap1-AU structure with high capping efficiency 
- 100% substitution with 5-methylcytidine (m5C) for improved protein expression and reduced innate immune response 
- Constructed using GenScript’s patented saRNA Vector 1 
- A 68A poly(A) tail to mimic natural mature mRNA
Properties
Form Liquid
Concentration 1mg/mL
Full mRNA length 9891nt
Full mRNA Molecular Weight 3441479
Storage Store at -20°C for short term (<3 months), store at -80°C for long term.
Storage buffer 1mM Sodium citrate, pH6.5

Quality Control Specifications
Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 70%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5

Applications
Transfect 0.01-0.05 µg of saRNA with 0.5 µL of Lipofectamine™ 2000 (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s protocol. Analyze the expression 16-48 hours post-transfection using a plate reader, flow cytometer, fluorescence microscope, or confocal microscope. For equipment settings, we recommend: 485 nm excitation, 535 nm emission.

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.